In vivo Multimodal Magnetic Particle Imaging for Early Detection of Ischemic Stroke in Tree Shrews.

IF 2.5 4区医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

Molecular Imaging and Biology Pub Date : 2025-08-06 DOI:10.1007/s11307-025-02031-2

Bo Zhang, Tao Zhu, Haoran Zhang, Xiaomei Yu, Jie He, Sijia Liu, Yanjun Liu, Zechen Wei, Chaoen Hu, Yali Zhang, Hongdi Huang, Minghao Qiu, Rui Jin, Hongli Li, Huiheng Xie, Jianhong Wang, Hui Hui, Jie Tian

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引用次数: 0

Abstract

Purpose: Ischemic stroke is a significant threat to human life and health, and timely diagnosis is essential for improving patient outcomes. Magnetic Particle Imaging (MPI), as an emerging high-sensitivity imaging technology, holds significant potential for the diagnosis of ischemic stroke. It is necessary to conduct multimodal MPI research based on the characteristics of the animal model and the detection needs of ischemic stroke.

Procedures: We used tree shrews, which have a close phylogenetic relationship with primates, as experimental subjects and established a photothrombotic (PT) stroke model. Considering the body size of tree shrews and the high-sensitivity detection requirements for ischemic stroke, a dedicated MPI receiving system for tree shrews was developed based on the primate brain MPI equipment. After validating the MPI system's performance, multimodal MPI fusion imaging of the tree shrew brain was performed by combining magnetic resonance imaging (MRI) and computed tomography (CT).

Results: The sensitivity of the receiving system for tree shrews is 0.017 mg Fe/mL, which is 8 times higher than that of the original system. Within one hour after the establishment of the PT stroke model, the MPI signal intensity in ischemic stroke tree shrews was approximately 25% lower than in the control group, while MRI showed no significant differences. On the 6th and 12th days after ischemic stroke onset, MRI images revealed clear lesion locations. Anatomical results of the tree shrew brain revealed significant lesions, confirming the successful establishment of the PT stroke model.

Conclusions: The dedicated MPI receiving system developed in this study significantly enhanced MPI sensitivity. The multimodal MPI imaging platform integrates the advantages of MRI and CT structural imaging based on high-sensitivity detection, enabling early detection of ischemic stroke in tree shrews.

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多模态磁粒子成像在树鼩缺血性中风早期检测中的应用。

目的：缺血性脑卒中是对人类生命和健康的重大威胁，及时诊断对改善患者预后至关重要。磁颗粒成像（MPI）作为一种新兴的高灵敏度成像技术，在缺血性脑卒中的诊断中具有重要的潜力。基于动物模型的特点和缺血性脑卒中的检测需求，开展多模态MPI研究是必要的。实验方法：我们以树鼩作为实验对象，建立了与灵长类动物有密切系统发育关系的光血栓性中风模型。考虑到树鼩的体型和对缺血性中风的高灵敏度检测要求，在灵长类动物脑MPI设备的基础上，研制了树鼩专用MPI接收系统。在验证了MPI系统的性能后，通过磁共振成像（MRI）和计算机断层扫描（CT）对树鼩脑进行多模态MPI融合成像。结果：该系统对树鼩的灵敏度为0.017 mg Fe/mL，比原系统提高了8倍。PT脑卒中模型建立后1小时内，缺血性脑卒中树鼩MPI信号强度较对照组降低约25%，而MRI无明显差异。在缺血性脑卒中发作后第6天和第12天，MRI图像显示明确的病变位置。树鼩脑解剖结果显示明显病变，证实PT脑卒中模型建立成功。结论：本研究开发的专用MPI接收系统显著提高了MPI敏感性。多模态MPI成像平台融合了基于高灵敏度检测的MRI和CT结构成像的优势，能够早期发现树鼩缺血性卒中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Imaging and Biology 医学-核医学

CiteScore

6.90

自引率

3.20%

发文量

审稿时长

3 months

期刊介绍： Molecular Imaging and Biology (MIB) invites original contributions (research articles, review articles, commentaries, etc.) on the utilization of molecular imaging (i.e., nuclear imaging, optical imaging, autoradiography and pathology, MRI, MPI, ultrasound imaging, radiomics/genomics etc.) to investigate questions related to biology and health. The objective of MIB is to provide a forum to the discovery of molecular mechanisms of disease through the use of imaging techniques. We aim to investigate the biological nature of disease in patients and establish new molecular imaging diagnostic and therapy procedures. Some areas that are covered are: Preclinical and clinical imaging of macromolecular targets (e.g., genes, receptors, enzymes) involved in significant biological processes. The design, characterization, and study of new molecular imaging probes and contrast agents for the functional interrogation of macromolecular targets. Development and evaluation of imaging systems including instrumentation, image reconstruction algorithms, image analysis, and display. Development of molecular assay approaches leading to quantification of the biological information obtained in molecular imaging. Study of in vivo animal models of disease for the development of new molecular diagnostics and therapeutics. Extension of in vitro and in vivo discoveries using disease models, into well designed clinical research investigations. Clinical molecular imaging involving clinical investigations, clinical trials and medical management or cost-effectiveness studies.