Microbiology and Immunology最新文献_第9页

The prevention effect of Limosilactobacillus reuteri on acute kidney injury by regulating gut microbiota 通过调节肠道微生物群来预防急性肾损伤的Limosilactobacillus reuteri。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2024-05-15 DOI: 10.1111/1348-0421.13130

Zhan Yang, Juan Ni, Xuewei Sun, Qian Cui, Xinrui Zhang, Mingyan Zhang, Xiaojing Zhu, Zihan Wu, Chengliang Tang, Jingfeng Zhu, Huijuan Mao, Kang Liu, Chunhui Wang, Changying Xing, Jin Zhu

{"title":"The prevention effect of Limosilactobacillus reuteri on acute kidney injury by regulating gut microbiota","authors":"Zhan Yang, Juan Ni, Xuewei Sun, Qian Cui, Xinrui Zhang, Mingyan Zhang, Xiaojing Zhu, Zihan Wu, Chengliang Tang, Jingfeng Zhu, Huijuan Mao, Kang Liu, Chunhui Wang, Changying Xing, Jin Zhu","doi":"10.1111/1348-0421.13130","DOIUrl":"10.1111/1348-0421.13130","url":null,"abstract":"Acute kidney injury (AKI) has considerably high morbidity and mortality but we do not have proper treatment for it. There is an urgent need to develop new prevention or treatment methods. Gut microbiota has a close connection with renal diseases and has become the new therapy target for AKI. In this study, we found the oral administration of the probiotic Limosilactobacillus reuteri had a prevention effect on the AKI induced by lipopolysaccharide (LPS). It reduced serum concentration of creatinine and urea nitrogen and protected the renal cells from necrosis and apoptosis. Meanwhile, L. reuteri improved the gut barrier function, which is destroyed in AKI, and modulated the gut microbiota and relevant metabolites. Compared with the LPS group, L. reuteri increased the proportion of Proteobacteria and reduced the proportion of Firmicutes, changing the overall structure of the gut microbiota. It also influenced the fecal metabolites and changed the metabolite pathways, such as tyrosine metabolism, pentose and glucuronate interconversions, galactose metabolism, purine metabolism, and insulin resistance. These results showed that L. reuteri is a potential therapy for AKI as it helps in sustaining the gut barrier integrity and modulating gut microbiota and related metabolites.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"68 7","pages":"213-223"},"PeriodicalIF":1.9,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140922774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information – Cover 发行信息 - 封面

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-05-06 DOI: 10.1111/1348-0421.13129

引用次数: 0

Possible link between colonization of the gastrointestinal tract by Citrobacter rodentium in C57BL/6 mice and microbiota composition C57BL/6 小鼠胃肠道啮齿动物柠檬杆菌定植与微生物群组成之间的可能联系。

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-04-21 DOI: 10.1111/1348-0421.13128

Tsuyoshi Miki, Takeshi Haneda, Nobuhiko Okada, Masahiro Ito

引用次数: 0

Proposal of Helicobacter higonensis sp. nov. isolated from a human clinical specimen, and emended description of Helicobacter valdiviensis Collado, 2014 从人类临床标本中分离出新的Helicobacter higonensis sp.的建议，以及对Helicobacter valdiviensis Collado, 2014的修订描述

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-04-10 DOI: 10.1111/1348-0421.13127

Junko Tomida, Tohru Miyoshi-Akiyama, Ryo Kutsuna, Hiroyasu Tsutsuki, Tomohiro Sawa, Margo Cnockaert, Peter Vandamme, Yoshiaki Kawamura

{"title":"Proposal of Helicobacter higonensis sp. nov. isolated from a human clinical specimen, and emended description of Helicobacter valdiviensis Collado, 2014","authors":"Junko Tomida, Tohru Miyoshi-Akiyama, Ryo Kutsuna, Hiroyasu Tsutsuki, Tomohiro Sawa, Margo Cnockaert, Peter Vandamme, Yoshiaki Kawamura","doi":"10.1111/1348-0421.13127","DOIUrl":"10.1111/1348-0421.13127","url":null,"abstract":"We have previously isolated a gram-negative microaerophilic strain, PAGU2000T from a patient presenting with a fever in Kumamoto Prefecture, Japan. The present study aimed to comprehensively analyze the taxonomy of the isolated strain using a polyphasic approach. The 16S rRNA gene sequence analysis indicated that the strain was a member of enterohepatic Helicobacter. The strain PAGU2000T shared a 97.5% 16S rRNA gene nucleotide identity with Helicobacter valdiviensis, and this taxonomic position was confirmed by phylogenetic analysis of the GyrA amino acid sequences. The proposed strain PAGU2000T has a 1.482 Mbp chromosome with a DNA G + C content of 31.3 mol% and encodes 1520 predicted coding sequences. The average nucleotide identity between the strain PAGU2000T and type strain of H. valdiviensis was 70.3%, which was lower than the recommended threshold of 95% for species delineation. The strain PAGU2000T was a motile, non-spore-forming, and spiral-shaped bacterium, exhibiting catalase and oxidase activities but not urease and nitrate reduction. This study demonstrates that the isolate represents a novel species within enterohepatic Helicobacter, for which the name Helicobacter higonensis is proposed (type strain: PAGU2000T = GTC 16811T = LMG 33095T). In this study, we describe the phenotypic and morphological features of this strain and propose an emended description of some biochemical traits of H. valdiviensis.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"68 6","pages":"197-205"},"PeriodicalIF":2.6,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140579812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information – Cover 发行信息 - 封面

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-04-09 DOI: 10.1111/1348-0421.13126

引用次数: 0

Issue Information – Cover 发行信息 - 封面

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-03-11 DOI: 10.1111/1348-0421.13125

引用次数: 0

Interferon inducible guanylate-binding protein 1 modulates the lipopolysaccharide-induced cytokines/chemokines and mitogen-activated protein kinases in macrophages 干扰素诱导鸟苷酸结合蛋白1可调节脂多糖诱导的细胞因子/凝血因子和巨噬细胞中的丝裂原活化蛋白激酶。

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-03-10 DOI: 10.1111/1348-0421.13123

Ravindra Kumar, Pramod Kumar Kushawaha

{"title":"Interferon inducible guanylate-binding protein 1 modulates the lipopolysaccharide-induced cytokines/chemokines and mitogen-activated protein kinases in macrophages","authors":"Ravindra Kumar, Pramod Kumar Kushawaha","doi":"10.1111/1348-0421.13123","DOIUrl":"10.1111/1348-0421.13123","url":null,"abstract":"Guanylate-binding proteins (GBPs) are a family of interferon (IFN)-inducible GTPases and play a pivotal role in the host immune response to microbial infections. These are upregulated in immune cells after recognizing the lipopolysaccharides (LPS), the major membrane component of Gram-negative bacteria. In the present study, the expression pattern of GBP1–7 was initially mapped in phorbol 12-myristate 13-acetate-differentiated human monocytes THP-1 and mouse macrophages RAW 264.7 cell lines stimulated with LPS. A time-dependent significant expression of GBP1–7 was observed in these cells. Moreover, among the various GBPs, GBP1 has emerged as a central player in regulating innate immunity and inflammation. Therefore, to study the specific role of GBP1 in LPS-induced inflammation, knockdown of the Gbp1 gene was carried out in both cells using small interfering RNA interference. Altered levels of different cytokines (interleukin [IL]-4, IL-10, IL-12β, IFN-γ, tumor necrosis factor-α), inducible nitric oxide synthase, histocompatibility 2, class II antigen A, protein kinase R, and chemokines (chemokine (C-X-C motif) ligand 9 [CXCL9], CXCL10, and CXCL11) in GBP1 knockdown cells were reported compared to control cells. Interestingly, the extracellular-signal-regulated kinase 1/2 mitogen-activated protein (MAP) kinases and signal transducer and activator of transcription 1 (STAT1) transcription factor levels were considerably induced in knockdown cells compared to the control cells. However, no change in the level of phosphorylated nuclear factor-kB, c-Jun, and p38 transcription factors was observed in GBP1 knockdown cells compared to the control cells. This study concludes that GBP1 may alter the expression of cytokines, chemokines, and effector molecules mediated by MAP kinases and STAT1 transcription factors.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"68 5","pages":"185-195"},"PeriodicalIF":2.6,"publicationDate":"2024-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140094377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N-glycosylation of the SARS-CoV-2 spike protein at Asn331 and Asn343 is involved in spike-ACE2 binding, virus entry, and regulation of IL-6 SARS-CoV-2尖峰蛋白在Asn331和Asn343处的N-糖基化参与了尖峰蛋白与ACE2的结合、病毒的进入和IL-6的调节。

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-03-06 DOI: 10.1111/1348-0421.13121

Tuhin Das, Shuhong Luo, Hao Tang, Jianmin Fang, Yinging Mao, Haw-Han Yen, Sabyasachi Dash, Asif Shajahan, Lauren Pepi, Steven Huang, Valerie S. Jones, Shehuo Xie, Gordon F. Huang, Jinqiao Lu, Blake Anderson, Benyue Zhang, Parastoo Azadi, Ruo-Pan Huang

{"title":"N-glycosylation of the SARS-CoV-2 spike protein at Asn331 and Asn343 is involved in spike-ACE2 binding, virus entry, and regulation of IL-6","authors":"Tuhin Das, Shuhong Luo, Hao Tang, Jianmin Fang, Yinging Mao, Haw-Han Yen, Sabyasachi Dash, Asif Shajahan, Lauren Pepi, Steven Huang, Valerie S. Jones, Shehuo Xie, Gordon F. Huang, Jinqiao Lu, Blake Anderson, Benyue Zhang, Parastoo Azadi, Ruo-Pan Huang","doi":"10.1111/1348-0421.13121","DOIUrl":"10.1111/1348-0421.13121","url":null,"abstract":"The coronavirus disease 2019 (COVID-19) pandemic is an ongoing global public health crisis. The causative agent, the SARS-CoV-2 virus, enters host cells via molecular interactions between the viral spike protein and the host cell ACE2 surface protein. The SARS-CoV-2 spike protein is extensively decorated with up to 66 N-linked glycans. Glycosylation of viral proteins is known to function in immune evasion strategies but may also function in the molecular events of viral entry into host cells. Here, we show that N-glycosylation at Asn331 and Asn343 of SARS-CoV-2 spike protein is required for it to bind to ACE2 and for the entry of pseudovirus harboring the SARS-CoV-2 spike protein into cells. Interestingly, high-content glycan binding screening data have shown that N-glycosylation of Asn331 and Asn343 of the RBD is important for binding to the specific glycan molecule G4GN (Galβ−1,4 GlcNAc), which is critical for spike-RBD-ACE2 binding. Furthermore, IL-6 was identified through antibody array analysis of conditioned media of the corresponding pseudovirus assay. Mutation of N-glycosylation of Asn331 and Asn343 sites of the spike receptor-binding domain (RBD) significantly reduced the transcriptional upregulation of pro-inflammatory signaling molecule IL-6. In addition, IL-6 levels correlated with spike protein levels in COVID-19 patients' serum. These findings establish the importance of RBD glycosylation in SARS-CoV-2 pathogenesis, which can be exploited for the development of novel therapeutics for COVID-19.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"68 5","pages":"165-178"},"PeriodicalIF":2.6,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.13121","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140039857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of COS-BK cells persistently infected with archetype BK polyomavirus 建立持续感染原型 BK 多瘤病毒的 COS-BK 细胞。

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-03-03 DOI: 10.1111/1348-0421.13124

Souichi Nukuzuma, Hiroshi Onogi, Tetsuro Suzuki

{"title":"Establishment of COS-BK cells persistently infected with archetype BK polyomavirus","authors":"Souichi Nukuzuma, Hiroshi Onogi, Tetsuro Suzuki","doi":"10.1111/1348-0421.13124","DOIUrl":"10.1111/1348-0421.13124","url":null,"abstract":"BK polyomavirus (BKPyV) was the first human polyomavirus to be isolated from an immunosuppressed kidney transplant recipient in 1971. BKPyV reactivation causes BKPyV-associated nephropathy and hemorrhagic cystitis. However, the mechanisms underlying BKPyV replication remain unclear. In the present study, we performed the long-term cultivation of COS-7 cells transfected with archetype KOM-5 DNA, which were designated as COS-BK cells. BKPyV derived from COS-BK cells was characterized by analyzing the amount of the virus based on hemagglutination, viral replication, and the production of viral protein 1 (VP1). Immunostaining showed that VP1-positive cells accounted for a small percentage of COS-BK cells. The nucleotide sequences encompassing the origin of the DNA replication of BKPyV derived from COS-BK cells were generated from KOM-5 by the deletion of an 8-bp sequence, which did not involve T antigen binding sites. BKPyV replicated most efficiently in COS-BK cells in DMEM containing 2% fetal bovine serum. These results indicate that COS-BK cells are a suitable culture system for studying the persistent infection of archetype BKPyV.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"68 5","pages":"179-184"},"PeriodicalIF":2.6,"publicationDate":"2024-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140022205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid quantitative detection system for measles virus-neutralizing antibodies using HiBiT-tagged virus-like particles 使用 HiBiT 标记病毒样颗粒的麻疹病毒中和抗体快速定量检测系统。

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-02-27 DOI: 10.1111/1348-0421.13122

Takashi Okura, Kei Miyakawa, Maino Tahara, Kenji Someya, Fumio Seki, Mayuko Nishi, Noriyuki Otsuki, Akihide Ryo

引用次数: 0