Microbiology and Immunology最新文献_第9页

Amentoflavone inhibits hepatitis B virus infection via the suppression of preS1 binding to host cells 阿门托黄酮通过抑制preS1与宿主细胞的结合抑制乙型肝炎病毒感染

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-03-16 DOI: 10.1111/1348-0421.13064

Chie Aoki-Utsubo, Puguh Indrasetiawan, Kento Fukano, Masamichi Muramatsu, Nina Artanti, Muhammad Hanafi, Hak Hotta, Masanori Kameoka

{"title":"Amentoflavone inhibits hepatitis B virus infection via the suppression of preS1 binding to host cells","authors":"Chie Aoki-Utsubo, Puguh Indrasetiawan, Kento Fukano, Masamichi Muramatsu, Nina Artanti, Muhammad Hanafi, Hak Hotta, Masanori Kameoka","doi":"10.1111/1348-0421.13064","DOIUrl":"10.1111/1348-0421.13064","url":null,"abstract":"Hepatitis B virus (HBV) is a leading cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Current therapeutic drugs for chronic HBV infection use IFN and nucleos(t)ide analogs; however, their efficacy is limited. Thus, there is an urgent need to develop new antivirals for HBV therapy. In this study, we identified a plant-derived polyphenolic bioflavonoid, amentoflavone, as a new anti-HBV compound. Amentoflavone treatment dose-dependently inhibited HBV infection in HBV-susceptible cells with HepG2-hNTCP-C4 and primary human hepatocyte PXB-cells. A mode-of-action study showed that amentoflavone inhibits the viral entry step, but not the viral internalization and early replication processes. Attachment of HBV particles as well as HBV preS1 peptide to HepG2-hNTCP-C4 cells was inhibited by amentoflavone. The transporter assay revealed that amentoflavone partly inhibits uptake of sodium taurocholate cotransporting polypeptide (NTCP)–mediated bile acid. Furthermore, effect of various amentoflavone analogs on HBs and HBe production from HBV-infected HepG2-hNTCP-C4 cells was examined. Robustaflavone exhibited comparable anti-HBV activity to that of amentoflavone and an amentoflavone-7,4', 4‴-trimethyl ether derivative (sciadopitysin) with moderate anti-HBV activity. Cupressuflavone or the monomeric flavonoid apigenin did not exhibit the antiviral activity. Amentoflavone and its structurally related biflavonoids may provide a potential drug scaffold in the design of a new anti-HBV drug inhibitor targeting NTCP.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9953549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Nicotine- and tar-removed cigarette smoke extract modulates the antigen presentation function of mouse bone marrow-derived dendritic cells 尼古丁和焦油去除香烟烟雾提取物调节小鼠骨髓来源树突状细胞的抗原呈递功能

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-03-09 DOI: 10.1111/1348-0421.13061

Kazuyuki Furuta, Takehiro Yoshioka, Kana Nishikaze, Noriko Yoshikawa, Kazuki Nakamura, Chikara Kaito

{"title":"Nicotine- and tar-removed cigarette smoke extract modulates the antigen presentation function of mouse bone marrow-derived dendritic cells","authors":"Kazuyuki Furuta, Takehiro Yoshioka, Kana Nishikaze, Noriko Yoshikawa, Kazuki Nakamura, Chikara Kaito","doi":"10.1111/1348-0421.13061","DOIUrl":"10.1111/1348-0421.13061","url":null,"abstract":"Dendritic cells (DCs) take up antigens derived from pathogens such as bacteria and viruses, and from tumor cells and induce the activation of antigen-specific T cells through major histocompatibility complex (MHC)-mediated antigen presentation. Mainstream cigarette smoke extract (CSE) has various effects, and the effects of its major components, nicotine and tar, have been analyzed extensively. Recently, the physiological effects of nicotine- and tar-removed CSE (cCSE) have also been reported. However, the effects of cCSE on DC-mediated immune responses remain unknown. In this study, we found that cCSE enhanced lipopolysaccharide (LPS)-stimulated induction of the expression of MHC-I and MHC-II on the cell surface of mouse bone marrow-derived DCs (BMDCs). In contrast, cCSE suppressed the induction of CD86 induced by stimulation with curdlan and interferon-γ (IFN-γ). In addition, cCSE suppressed the production of IL-12, IL-23, and IL-10 by LPS and curdlan stimulation. In the presence of cCSE, LPS-stimulated BMDCs showed enhanced activation of CD4 and CD8 T cells and increased IL-2 production from T cells by antigen presentation in a mixed-leukocyte reaction assay. In contrast, cCSE did not affect the activation of T cells by curdlan- or IFN-γ-stimulated BMDCs, and curdlan-stimulated BMDCs suppressed IL-17 production from T cells and enhanced IFN-γ production. These results suggest that cCSE has different effects on the activation signals induced by LPS, curdlan, and IFN-γ in BMDCs and modulates the antigen presentation function of BMDCs.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9755557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in studies on magnetosome-associated proteins composing the bacterial geomagnetic sensor organelle 细菌地磁传感细胞器磁小体相关蛋白的研究进展

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-03-09 DOI: 10.1111/1348-0421.13062

Azuma Taoka, Yukako Eguchi, Rino Shimoshige, Yoshihiro Fukumori

引用次数: 0

Molecular mechanism of Streptococcus pneumoniae–targeting xenophagy recognition and evasion: Reinterpretation of pneumococci as intracellular bacteria 肺炎链球菌靶向异食识别和逃避的分子机制:肺炎球菌作为细胞内细菌的重新解释

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-03-05 DOI: 10.1111/1348-0421.13060

Michinaga Ogawa, Sayaka Shizukuishi, Yukihiro Akeda, Makoto Ohnishi

{"title":"Molecular mechanism of Streptococcus pneumoniae–targeting xenophagy recognition and evasion: Reinterpretation of pneumococci as intracellular bacteria","authors":"Michinaga Ogawa, Sayaka Shizukuishi, Yukihiro Akeda, Makoto Ohnishi","doi":"10.1111/1348-0421.13060","DOIUrl":"10.1111/1348-0421.13060","url":null,"abstract":"Streptococcus pneumoniae is a major, encapsulated Gram-positive pathogen that causes diseases including community-acquired pneumonia, meningitis, and sepsis. This pathogen colonizes the nasopharyngeal epithelia asymptomatically but can often migrate to sterile tissues and cause life-threatening invasive infections (invasive pneumococcal disease). Although multivalent pneumococcal polysaccharides and conjugate vaccines are available and effective, they also have major shortcomings with respect to the emergence of vaccine-resistant serotypes. Therefore, alternative therapeutic approaches are needed, and the molecular analysis of host–pathogen interactions and their applications to pharmaceutical development and clinical practice has recently received increased attention. In this review, we introduce pneumococcal surface virulence factors involved in pathogenicity and highlight recent advances in our understanding of host autophagy recognition mechanisms against intracellular S. pneumoniae and pneumococcal evasion from autophagy.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9459248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Issue Information – Cover 问题信息-封面

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-03-03 DOI: 10.1111/1348-0421.12991

引用次数: 0

γδ T-cell–mediated immune responses to malaria γδ t细胞介导的疟疾免疫应答

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-02-24 DOI: 10.1111/1348-0421.13059

Ganchimeg Bayarsaikhan, Yarob Ibraheem, Shin-Ichi Inoue

{"title":"γδ T-cell–mediated immune responses to malaria","authors":"Ganchimeg Bayarsaikhan, Yarob Ibraheem, Shin-Ichi Inoue","doi":"10.1111/1348-0421.13059","DOIUrl":"10.1111/1348-0421.13059","url":null,"abstract":"Malaria is one of the deadliest infectious diseases. Licensed vaccine have demonstrated just over 30% efficacy, and therefore, developing new vaccine candidates and understanding immune responses to Plasmodium have become necessary. γδ T cells have been suggested to be associated with immune responses to malaria due to the observation of their expansion in patients with malaria and experimental models of malaria. γδ T cells act as both “innate-like” and “adaptive-like” cells during immune response to malaria. Studies have found that γδ T cells can recognize Plasmodium phosphoantigen, present the antigen, and initiate adaptive immune response during blood-stage Plasmodium infection. Recent reports also suggested the phagocytic and cytotoxic potential of γδ T cells. Furthermore, γδ T cells can provide protection upon immunization with whole parasite. In addition, γδ T cells during the liver-stage infection were able to prevent experimental cerebral malaria. Despite these new findings, questions related to γδ T-cell response during Plasmodium infection remain to be answered. However, investigating these cells in humans remains difficult in many ways; in this regard, rodent models of malarial infection enable us to study these cells in more detail. Insights from experimental malaria models give rise to new cues for development of malarial vaccine and adjunctive therapy for severe malaria. Here, we review our current knowledge of γδ T-cell immune function in human and experimental mouse malarial infection models; especially, we focus on the mechanisms underlying γδ T cells that are associated with protective immunity during malarial infection.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9755541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Premarital screening of the viral hepatitis among Saudi nationals 沙特国民病毒性肝炎的婚前筛查

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-02-22 DOI: 10.1111/1348-0421.13058

Abdullah A. K. Alzahrani, Yasser A. Altalhi, Ahmed A. S. Alghamdi, Samah M. Muhandis, Daifallah M. Al Aboud, Gaber M. G. Shehab, Ahmed S. Abdel-Moneim

{"title":"Premarital screening of the viral hepatitis among Saudi nationals","authors":"Abdullah A. K. Alzahrani, Yasser A. Altalhi, Ahmed A. S. Alghamdi, Samah M. Muhandis, Daifallah M. Al Aboud, Gaber M. G. Shehab, Ahmed S. Abdel-Moneim","doi":"10.1111/1348-0421.13058","DOIUrl":"10.1111/1348-0421.13058","url":null,"abstract":"Blood borne sexually transmitted infections are among the most serious health problems worldwide. Many people possessing these infections do not have symptoms and may remain undiagnosed. The current study aimed to screen premaritally the incidence of blood borne viruses among Saudi nationals. A retrospective longitudinal study was conducted, using a total of 91,000 medical records, in the blood bank from a single center in the Western region of Saudi Arabia. All persons who underwent premarital examination during the period 2016–2021 for the presence of hepatitis B and C viruses as a part of the national screening program in Saudi Arabia were included in the study. Serological tests were used to screen the presence of HBc Ab and HBs Ag. Both anti-HCV antibodies and the presence of virus RNA using real-time reverse transcriptase polymerase chain reaction (RT-PCR) were also performed. The study reported the presence of 378/91000 (0.42%) infections with hepatitis B virus (HBV) as indicated by the presence of HBc Ab and HBs Ag. Meanwhile, 208 (0.23%) cases were found to be exposed to HCV including 49/91000 (0.05%) active HCV cases, positive for the HCV RNA, while 159/91000 (0.17%) persons were found to possess positive HCV antibodies in the absence of detectable HCV RNA. It was concluded that there is a low prevalence of HBV and HBV among Saudi citizens who were subjected to premarital screening.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9395577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The autotransporter BafA contributes to the proangiogenic potential of Bartonella elizabethae 自体转运体BafA有助于伊丽莎白巴尔通体的促血管生成潜能

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-02-22 DOI: 10.1111/1348-0421.13057

Natsumi Suzuki, Kayo Kumadaki, Kaoru Tatematsu, Yohei Doi, Kentaro Tsukamoto

{"title":"The autotransporter BafA contributes to the proangiogenic potential of Bartonella elizabethae","authors":"Natsumi Suzuki, Kayo Kumadaki, Kaoru Tatematsu, Yohei Doi, Kentaro Tsukamoto","doi":"10.1111/1348-0421.13057","DOIUrl":"10.1111/1348-0421.13057","url":null,"abstract":"Bartonella elizabethae is a rat-borne zoonotic bacterium that causes human infectious endocarditis or neuroretinitis. Recently, a case of bacillary angiomatosis (BA) resulting from this organism was reported, leading to speculation that B. elizabethae may also trigger vasoproliferation. However, there are no reports of B. elizabethae promoting human vascular endothelial cell (EC) proliferation or angiogenesis, and to date, the effects of this bacterium on ECs are unknown. We recently identified a proangiogenic autotransporter, BafA, secreted from B. henselae and B. quintana, which are recognized as Bartonella spp. responsible for BA in humans. Here, we hypothesized that B. elizabethae also harbored a functional bafA gene and examined the proangiogenic activity of recombinant B. elizabethae–derived BafA. The bafA gene of B. elizabethae, which was found to share a 51.1% amino acid sequence identity with BafA of B. henselae and 52.5% with that of B. quintana in the passenger domain, was located in a syntenic region of the genome. The recombinant protein of the N-terminal passenger domain of B. elizabethae-BafA facilitated EC proliferation and capillary structure formation. Furthermore, it upregulated the receptor signaling pathway of vascular endothelial growth factor, as observed in B. henselae-BafA. Taken together, B. elizabethae–derived BafA stimulates human EC proliferation and may contribute to the proangiogenic potential of this bacterium. So far, functional bafA genes have been found in all BA-causing Bartonella spp., supporting the key role BafA may play in BA pathogenesis.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9403343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Promising whole-cell vaccines against cryptococcosis 有前途的全细胞疫苗对抗隐球菌病

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-02-14 DOI: 10.1111/1348-0421.13056

Keigo Ueno, Soichiro Tsuge, Kiminori Shimizu, Yoshitsugu Miyazaki

{"title":"Promising whole-cell vaccines against cryptococcosis","authors":"Keigo Ueno, Soichiro Tsuge, Kiminori Shimizu, Yoshitsugu Miyazaki","doi":"10.1111/1348-0421.13056","DOIUrl":"10.1111/1348-0421.13056","url":null,"abstract":"Cryptococcosis is a mycosis caused by Cryptococcus neoformans and C. gattii species complexes. Although this infection is potentially lethal, no prophylactic vaccine is yet commercially available, and the immune memory that enables prevention is still under investigation. These pathogens have a capsule layer for immune evasion and a sophisticated mechanism to advance the infection, and it is expected that these characteristics will make it difficult to develop prophylactic vaccines and to decipher the protective immunity. The current vaccine studies are focused on subunit, mRNA, DNA, and viral vector vaccines, with whole-cell vaccines also proving successful against cryptococcal infections. Cryptococcal whole-cell vaccines have been composed of highly immunostimulating strains with low-pathogenicity that are modified by genetic recombination technology. Examples include the whole-cell vaccines H99γ, sgl1∆, fbp1∆, znf2oe, cda1/2/3∆, cap59∆, and cap60∆. Some of these whole-cell vaccines were found to be highly effective in prolonging life and suppressing the fungal burden after an infection challenge in mice, and to be cross-reactive to C. neoformans, C. gattii, and other fungal pathogens. Furthermore, for some vaccines, the protective effect can be retained even in an immunocompromised host depleted of CD4+ T cells. These findings have provided new insights into protective immunity that should aid in vaccine development. In this review, we highlight the upsides and downsides of whole-cell vaccines against cryptococcosis.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9755529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Issue Information – Cover 问题信息-封面

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-02-04 DOI: 10.1111/1348-0421.12989

引用次数: 0