Microbiology and Immunology最新文献_第9页

Nicotine promotes Mycobacterium tuberculosis H37Rv growth and overexpression of virulence genes 尼古丁促进结核分枝杆菌H37Rv的生长和毒力基因的过表达

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-06-28 DOI: 10.1111/1348-0421.13085

Bruno Rivas-Santiago, Jeny de Haro-Acosta, Adrian Rodríguez Carlos, Mariana H. Garcia-Hernandez, Carmen J. Serrano, Irma Gonzalez-Curiel, Cesar Rivas-Santiago

{"title":"Nicotine promotes Mycobacterium tuberculosis H37Rv growth and overexpression of virulence genes","authors":"Bruno Rivas-Santiago, Jeny de Haro-Acosta, Adrian Rodríguez Carlos, Mariana H. Garcia-Hernandez, Carmen J. Serrano, Irma Gonzalez-Curiel, Cesar Rivas-Santiago","doi":"10.1111/1348-0421.13085","DOIUrl":"10.1111/1348-0421.13085","url":null,"abstract":"Tobacco consumption increases the susceptibility to develop infectious diseases such as tuberculosis (TB). Nicotine (Nc) is the main component of cigarette smoke with immunomodulatory properties, however, its effect on Mycobacterium tuberculosis (Mtb) has been scarcely investigated. The present study evaluated the effect of nicotine on the growth of Mtb and on the induction of virulence-related genes. Mycobacteria were exposed to different concentrations of nicotine then Mtb growth was evaluated. Subsequently, the expression of the virulence-related genes lysX, pirG, fad26, fbpa, ompa, hbhA, esxA, esxB, hspx, katG, lpqh, and caeA was evaluated by RT-qPCR. The effect of nicotine on intracellular Mtb was also evaluated. The results showed that nicotine promotes the growth of Mtb both extracellularly and intracellularly and increases the expression of genes related to virulence. In summary, nicotine promotes the growth of Mtb and the expression of virulence-related genes that could be correlated with the increased the risk of smokers developing TB.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 8","pages":"365-376"},"PeriodicalIF":2.6,"publicationDate":"2023-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10317991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Huoxue Jiedu Recipe represses mitochondrial fission to alleviate submandibular gland inflammation in Sjögren's syndrome 活血解毒方抑制线粒体裂变缓解Sjögren综合征颌下腺炎症

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-06-20 DOI: 10.1111/1348-0421.13084

Jiangxi Xu, Zhengdong Shen, Yun Du, Shai Zhang, Yajing Xi, Yuting Zhang, Dan Wang, Yuelan Zhu

{"title":"Huoxue Jiedu Recipe represses mitochondrial fission to alleviate submandibular gland inflammation in Sjögren's syndrome","authors":"Jiangxi Xu, Zhengdong Shen, Yun Du, Shai Zhang, Yajing Xi, Yuting Zhang, Dan Wang, Yuelan Zhu","doi":"10.1111/1348-0421.13084","DOIUrl":"10.1111/1348-0421.13084","url":null,"abstract":"Sjögren's syndrome (SS) is the second most common autoimmune rheumatism. Huoxue Jiedu Recipe (HXJDR) is a kind of traditional Chinese medicine with a variety of pharmacological functions; however, its biological function in SS has not been studied yet. Peripheral blood mononuclear cells (PBMCs) and serum samples were isolated from healthy controls and patients with SS. NOD/Ltj mice were used for developing the SS mouse model. The levels of inflammatory cytokines and NOD-like receptor family pyrin domain containing 3 (NLRP3) inflammasome–related markers as well as dynamin-related protein 1 (Drp1) were determined by ELISA, quantitative real-time PCR, and western blot analysis, respectively. Hematoxylin and eosin and TUNEL staining detected the pathological damage. A transmission electron microscope was used to observe the mitochondrial microstructure. Inflammatory cytokines IL-18, IL-1β, B-cell activating factor (BAFF), BAFF-receptor (BAFF-R), IL-6, and TNF-α in serum samples and NLRP3 inflammasome–related makers (NLRP3, cysteinyl aspartate–specific proteinase 1 [caspase-1], apoptosis-associated speck-like protein containing a caspase-1 recruitment domain [ASC], IL-1β) in PBMCs were greatly upregulated in patients with SS. Furthermore, cytoplasmic phosphorylation of Drp1 and mitochondrial Drp1 level were significantly increased in PBMCs, while mitochondrial swelling and fuzzy inner ridge were observed in PBMCs of patients with SS, suggesting increased mitochondrial fission. Compared with control mice, SS mice showed decreased salivary flow rate, increased submandibular gland index, and more severe inflammatory infiltration and damage as well as mitochondrial fission in submandibular gland tissues. After HXJDR administration, these effects were significantly reversed. HXJDR treatment could alleviate the inflammatory infiltration and pathological damage in submandibular glands of SS mice by inhibiting Drp-1–dependent mitochondrial fission.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 8","pages":"377-387"},"PeriodicalIF":2.6,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9944275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-recognition of innate immune memory as an integrated multidimensional concept 重新认识先天免疫记忆作为一个综合的多维概念

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-06-13 DOI: 10.1111/1348-0421.13083

Chang Tang, Shoichiro Kurata, Naoyuki Fuse

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Issue Information – Cover 问题信息-封面

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-06-05 DOI: 10.1111/1348-0421.12997

引用次数: 0

Capture and neutralization of SARS-CoV-2 and influenza virus by algae-derived lectins with high-mannose and core fucose specificities 利用具有高甘露糖和核心焦点特异性的藻类衍生凝集素捕获和中和SARS-CoV-2和流感病毒

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-05-29 DOI: 10.1111/1348-0421.13082

Tanuza Nazmul, Basirat M. Lawal-Ayinde, Tomoko Morita, Reiko Yoshimoto, Akifumi Higashiura, Akima Yamamoto, Toshihito Nomura, Yukiko Nakano, Makoto Hirayama, Hiroshi Kurokawa, Yasuyuki Kitamura, Kanji Hori, Takemasa Sakaguchi

{"title":"Capture and neutralization of SARS-CoV-2 and influenza virus by algae-derived lectins with high-mannose and core fucose specificities","authors":"Tanuza Nazmul, Basirat M. Lawal-Ayinde, Tomoko Morita, Reiko Yoshimoto, Akifumi Higashiura, Akima Yamamoto, Toshihito Nomura, Yukiko Nakano, Makoto Hirayama, Hiroshi Kurokawa, Yasuyuki Kitamura, Kanji Hori, Takemasa Sakaguchi","doi":"10.1111/1348-0421.13082","DOIUrl":"10.1111/1348-0421.13082","url":null,"abstract":"We first investigated the interactions between several algae-derived lectins and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). We created lectin columns using high-mannose (HM)–type glycan-specific lectins OAA and KAA-1 or core fucose–specific lectin hypninA-2 and conducted binding experiments with SARS-CoV-2. The results showed that these lectins were capable of binding to the virus. Furthermore, when examining the neutralization ability of nine different lectins, it was found that KAA-1, ESA-2, and hypninA-2 were effective in neutralizing SARS-CoV-2. In competitive inhibition experiments with glycoproteins, neutralization was confirmed to occur through HM-type or core fucose–type glycans. However, neutralization was not observed with other lectins, such as OAA. This trend of KAA-1 and ESA-2 having the neutralizing ability and OAA not having it was also similar to influenza viruses. Electron microscopy observations revealed that KAA-1 and hypninA-2 strongly aggregated SARS-CoV-2 particles, while OAA showed a low degree of aggregation. It is believed that the neutralization of SARS-CoV-2 involves multiple factors, such as glycan attachment sites on the S protein, the size of lectins, and their propensity to aggregate, which cause inhibition of receptor binding or aggregation of virus particles. This study demonstrated that several algae-derived lectins could neutralize SARS-CoV-2 and that lectin columns can effectively recover and concentrate the virus.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 7","pages":"334-344"},"PeriodicalIF":2.6,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9740362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Involvement of ribonuclease Y in pilus production by M49 Streptococcus pyogenes strain via modulation of messenger RNA level of transcriptional regulator 核糖核酸酶Y通过调节转录调节因子的信使RNA水平参与M49化脓性链球菌菌毛的产生

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-05-03 DOI: 10.1111/1348-0421.13069

Seiko Kubota, Masanobu Nakata, Yujiro Hirose, Masaya Yamaguchi, Bernd Kreikemeyer, Narikazu Uzawa, Tomoko Sumitomo, Shigetada Kawabata

{"title":"Involvement of ribonuclease Y in pilus production by M49 Streptococcus pyogenes strain via modulation of messenger RNA level of transcriptional regulator","authors":"Seiko Kubota, Masanobu Nakata, Yujiro Hirose, Masaya Yamaguchi, Bernd Kreikemeyer, Narikazu Uzawa, Tomoko Sumitomo, Shigetada Kawabata","doi":"10.1111/1348-0421.13069","DOIUrl":"10.1111/1348-0421.13069","url":null,"abstract":"Streptococcus pyogenes displays a wide variety of pili, which is largely dependent on serotype. A distinct subset of S. pyogenes strains that possess the Nra transcriptional regulator demonstrates thermoregulated pilus production. Findings obtained in the present study of an Nra-positive serotype M49 strain revealed involvement of conserved virulence factor A (CvfA), also referred to as ribonuclease Y (RNase Y), in virulence factor expression and pilus production, while a cvfA deletion strain showed reduced pilus production and adherence to human keratinocytes as compared with wild-type and revertant strains. Furthermore, transcript levels of pilus subunits and srtC2 genes were decreased by cvfA deletion, which was remarkable at 25°C. Likewise, both messenger RNA (mRNA) and protein levels of Nra were remarkably decreased by cvfA deletion. Whether the expression of other pilus-related regulators, including fasX and CovR, was subject to thermoregulation was also examined. While the mRNA level of fasX, which inhibits cpa and fctA translation, was decreased by cvfA deletion at both 37°C and 25°C, CovR mRNA and protein levels, as well as its phosphorylation level were not significantly changed, suggesting that neither fasX nor CovR is necessarily involved in thermosensitive pilus production. Phenotypic analysis of the mutant strains revealed that culture temperature and cvfA deletion had varied effects on streptolysin S and SpeB activities. Furthermore, bactericidal assay data showed that cvfA deletion decreased the rate of survival in human blood. Together, the present findings indicate that CvfA is involved in regulation of pilus production and virulence-related phenotypes of the serotype M49 strain of S. pyogenes.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 7","pages":"319-333"},"PeriodicalIF":2.6,"publicationDate":"2023-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10118652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information – Cover 问题信息-封面

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-05-02 DOI: 10.1111/1348-0421.12995

{"title":"Issue Information – Cover","authors":"","doi":"10.1111/1348-0421.12995","DOIUrl":"https://doi.org/10.1111/1348-0421.12995","url":null,"abstract":"Cover photograph: (1) The role of γδ T cells in skin is still unknown. Do they interact with sporozoite? Do they able to kill sporozoite or facilitate DC to recognize and phagocytize sporozoite? Lots to be answered in the future. (2) The responses of γδ T cells during liver-stage are mainly protective. Studies of attenuated sporozoite vaccination showed activation and expansion of γδ T cells. These γδ T cells produce large amount of IFN-γ and were able to protect subsequent challenge infection in human. γδ T cells are involved in activation and maturation of DC, and they may facilitate recruitment of inflammatory cells in the liver. γδ T cells also contribute to the activation and accumulation of CD8+ T cells directly or through DC. (3) Protective and pathogenic roles of γδ T cells during blood-stage have been proposed. Upon activation γδ T cells produce pro-inflammatory cytokines which enhance APC cells maturation. Also they are involved in accumulation and activation of myeloid cells by producing M-CSF. They can present antigens to naïve γδ T cells and initiate adaptive immune response. Moreover, γδ T cells facilitate antibody production and germinal center formation either directly contacting with B cells or through T follicular helper cells. In vitro studies of human γδ T cells showed capacity of antigen presentation, phagocytosis and cytotoxicity. However, these functions should be investigated in the tissue or in vivo. Microbiol Immunol: 67:239–247. Article link here\u0000 \u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture>\u0000 </div>\u0000 </figure>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 5","pages":"i-ii"},"PeriodicalIF":2.6,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.12995","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50121992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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A basic charge cluster near the C-terminus of the cytoplasmic tail contributes to the molecular stability of human herpesvirus 8 E3 ubiquitin ligases 细胞质尾部c端附近的一个基本电荷簇有助于人疱疹病毒8e3泛素连接酶的分子稳定性

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-04-17 DOI: 10.1111/1348-0421.13068

Mizuho Kajikawa, Koya Kato, Hayato Takahashi, Seiya Kitajima, Yosuke Kusunoki, Mizuki Haga, Minako Kimura, Yoshihiro Inoue, Kei Miyano, Taisei Kanamoto

{"title":"A basic charge cluster near the C-terminus of the cytoplasmic tail contributes to the molecular stability of human herpesvirus 8 E3 ubiquitin ligases","authors":"Mizuho Kajikawa, Koya Kato, Hayato Takahashi, Seiya Kitajima, Yosuke Kusunoki, Mizuki Haga, Minako Kimura, Yoshihiro Inoue, Kei Miyano, Taisei Kanamoto","doi":"10.1111/1348-0421.13068","DOIUrl":"10.1111/1348-0421.13068","url":null,"abstract":"Human herpesvirus 8 (HHV8; also known as Kaposi's sarcoma-associated herpesvirus [KSHV]) utilizes the viral E3 ubiquitin ligase family members K3 and K5 for immune evasion. Both K3 and K5 mediate the ubiquitination of host MHC class I (MHC-I) molecules, which play a key role in antigen presentation to cytotoxic T lymphocytes (CTLs). Because ubiquitinated MHC-I is immediately down-regulated from the cell surface, HHV8-infected cells can escape surveillance by CTLs. K3 and K5 have similar domain structures and topologies. They contain an N-terminal RINGv ubiquitin ligase domain, two transmembrane helices, and an intrinsically disordered cytoplasmic tail at the C-terminus. The cytoplasmic tail contains a membrane-proximal “conserved region” involved in ligase activity. On the other hand, the role of the membrane-distal region of the cytoplasmic tail, termed the “C-tail” in this study, remains unclear. Here, we demonstrate that the C-tail contributes to the protein expression of both K3 and K5. The C-tail-truncated K3 and K5 mutants were rapidly reduced in cells. The recombinant C-tail proteins bind to acidic lipids via a basic charge cluster located near the C-terminus of the C-tails. Similar to the C-tail-truncated mutants, the basic charge cluster-substituting mutants showed decreased protein expression of K3 and K5. These findings suggest that the basic charge cluster near the C-terminus of the cytoplasmic tail contributes to the molecular stability of K3 and K5.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 6","pages":"293-302"},"PeriodicalIF":2.6,"publicationDate":"2023-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9937755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information – Cover 问题信息-封面

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-04-05 DOI: 10.1111/1348-0421.12993

引用次数: 0

Phylogenic position and low genomic diversity of “Candidatus Rickettsia kotlanii” inferred by complete genome sequences of two Japanese isolates 由两个日本分离株的全基因组序列推断的“科特兰氏立克次体候选菌”的系统发育位置和低基因组多样性

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-03-30 DOI: 10.1111/1348-0421.13065

Yasuhiro Gotoh, Ai Takano, Masako Andoh, Kentaro Kasama, Keiji Nakamura, Shuji Ando, Tetsuya Hayashi

{"title":"Phylogenic position and low genomic diversity of “Candidatus Rickettsia kotlanii” inferred by complete genome sequences of two Japanese isolates","authors":"Yasuhiro Gotoh, Ai Takano, Masako Andoh, Kentaro Kasama, Keiji Nakamura, Shuji Ando, Tetsuya Hayashi","doi":"10.1111/1348-0421.13065","DOIUrl":"10.1111/1348-0421.13065","url":null,"abstract":"Many Rickettsia species of the spotted fever group (SFG) cause tick-borne diseases known as “spotted fever.” One of the candidate SFG Rickettsia species is “Candidatus Rickettsia kotlanii,” which was first detected in Haemaphysalis concinna in Hungary in 2006. However, its precise phylogenetic position in the SFG is not clear because only single-gene sequence–based phylogenetic analyses were performed using very limited genes. Here, we present the complete genome sequences of two Japanese “Ca. R. kotlanii” isolates, which differed only by a 135 bp insertion/deletion (InDel). Using these genomes and publicly available whole genome sequences of other Rickettsia species, the precise phylogenetic position of “Ca. R. kotlanii” in Rickettsia was determined to be in a clade of the SFG. The phylogenetic relationships and average nucleotide identity of “Ca. R. kotlanii” relative to the other species indicated that “Ca. R. kotlanii” is an independent taxon in the SFG. Notably, although the genomes of the two isolates were almost identical, the isolates were obtained from different tick species in different regions and years, suggesting extremely low genomic diversity in “Ca. R. kotlanii.” While the genome of “Ca. R. kotlanii” is the smallest in the transitional group and SFG Rickettsia sequenced to date, we identified genes uniquely present or absent in “Ca. R. kotlanii,” but most were apparently degraded. Therefore, analyses of differences at the sequence (single nucleotide polymorphisms and small InDels) or gene expression level will be required to understand the functional or physiological features unique to “Ca. R. kotlanii.”","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 6","pages":"275-280"},"PeriodicalIF":2.6,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.13065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9989288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0