{"title":"日本一家未明显爆发耐万古霉素肠球菌疫情的医院从住院病人中分离的肠球菌的抗生素敏感性和基因组分析。","authors":"Ayumi Fujii, Miki Kawada-Matsuo, Mi Nguyen-Tra Le, Kanako Masuda, Kayoko Tadera, Yujin Suzuki, Saki Nishihama, Junzo Hisatsune, Yo Sugawara, Seiya Kashiyama, Hideki Shiba, Tomonao Aikawa, Hiroki Ohge, Motoyuki Sugai, Hitoshi Komatsuzawa","doi":"10.1111/1348-0421.13155","DOIUrl":null,"url":null,"abstract":"<p>To prevent nosocomial infection, it is important to screen for potential vancomycin-resistant <i>Enterococcus</i> (VRE) among patients. In this study, we analyzed enterococcal isolates from inpatients in one hospital without any apparent outbreak of VRE. Enterococcal isolates were collected from inpatients at Hiroshima University Hospital from April 1 to June 30, 2021 using selective medium for Enterococci. Multilocus sequence typing, antimicrobial susceptibility testing, and whole-genome sequencing were performed. A total of 164 isolates, including <i>Enterococcus faecium</i> (41 isolates), <i>Enterococcus faecalis</i> (80 isolates), <i>Enterococcus raffinosus</i> (11 isolates), <i>Enterococcus casseliflavus</i> (nine isolates), <i>Enterococcus avium</i> (12 isolates), <i>Enterococcus lactis</i> (eight isolates), <i>Enterococcus gallinarum</i> (two isolates), and <i>Enterococcus malodoratus</i> (one isolate), were analyzed. We found one <i>vanA</i>-positive <i>E. faecium</i>, which was already informed when the patient was transferred to the hospital, nine <i>vanC</i>-positive <i>E. casseliflavus</i>, and two <i>vanC</i>-positive <i>E. gallinarum. E. faecium</i> isolates showed resistance to ampicillin (95.1%), imipenem (95.1%), and levofloxacin (87.8%), and <i>E. faecalis</i> isolates showed resistance to minocycline (49.4%). Ampicillin- and levofloxacin-resistant <i>E. faecium</i> had multiple mutations in penicillin-binding protein 5 (PBP5) (39/39 isolates) and ParC/GyrA (21/36 isolates), respectively. <i>E. raffinosus</i> showed resistance to ampicillin (81.8%), imipenem (45.5%), and levofloxacin (45.5%), and <i>E. lactis</i> showed resistance to ampicillin (37.5%) and imipenem (50.0%). The linezolid resistance genes <i>optrA</i> and <i>cfr</i>(B) were found only in one isolate of <i>E. faecalis</i> and <i>E. raffinosus</i>, respectively. This study, showing the status of enterococci infection in hospitalized patients, is one of the important information when considering nosocomial infection control of VRE.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"68 8","pages":"254-266"},"PeriodicalIF":1.9000,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antibiotic susceptibility and genome analysis of Enterococcus species isolated from inpatients in one hospital with no apparent outbreak of vancomycin-resistant Enterococcus in Japan\",\"authors\":\"Ayumi Fujii, Miki Kawada-Matsuo, Mi Nguyen-Tra Le, Kanako Masuda, Kayoko Tadera, Yujin Suzuki, Saki Nishihama, Junzo Hisatsune, Yo Sugawara, Seiya Kashiyama, Hideki Shiba, Tomonao Aikawa, Hiroki Ohge, Motoyuki Sugai, Hitoshi Komatsuzawa\",\"doi\":\"10.1111/1348-0421.13155\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>To prevent nosocomial infection, it is important to screen for potential vancomycin-resistant <i>Enterococcus</i> (VRE) among patients. In this study, we analyzed enterococcal isolates from inpatients in one hospital without any apparent outbreak of VRE. Enterococcal isolates were collected from inpatients at Hiroshima University Hospital from April 1 to June 30, 2021 using selective medium for Enterococci. Multilocus sequence typing, antimicrobial susceptibility testing, and whole-genome sequencing were performed. A total of 164 isolates, including <i>Enterococcus faecium</i> (41 isolates), <i>Enterococcus faecalis</i> (80 isolates), <i>Enterococcus raffinosus</i> (11 isolates), <i>Enterococcus casseliflavus</i> (nine isolates), <i>Enterococcus avium</i> (12 isolates), <i>Enterococcus lactis</i> (eight isolates), <i>Enterococcus gallinarum</i> (two isolates), and <i>Enterococcus malodoratus</i> (one isolate), were analyzed. We found one <i>vanA</i>-positive <i>E. faecium</i>, which was already informed when the patient was transferred to the hospital, nine <i>vanC</i>-positive <i>E. casseliflavus</i>, and two <i>vanC</i>-positive <i>E. gallinarum. E. faecium</i> isolates showed resistance to ampicillin (95.1%), imipenem (95.1%), and levofloxacin (87.8%), and <i>E. faecalis</i> isolates showed resistance to minocycline (49.4%). Ampicillin- and levofloxacin-resistant <i>E. faecium</i> had multiple mutations in penicillin-binding protein 5 (PBP5) (39/39 isolates) and ParC/GyrA (21/36 isolates), respectively. <i>E. raffinosus</i> showed resistance to ampicillin (81.8%), imipenem (45.5%), and levofloxacin (45.5%), and <i>E. lactis</i> showed resistance to ampicillin (37.5%) and imipenem (50.0%). The linezolid resistance genes <i>optrA</i> and <i>cfr</i>(B) were found only in one isolate of <i>E. faecalis</i> and <i>E. raffinosus</i>, respectively. This study, showing the status of enterococci infection in hospitalized patients, is one of the important information when considering nosocomial infection control of VRE.</p>\",\"PeriodicalId\":18679,\"journal\":{\"name\":\"Microbiology and Immunology\",\"volume\":\"68 8\",\"pages\":\"254-266\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-06-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiology and Immunology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/1348-0421.13155\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology and Immunology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1348-0421.13155","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
Antibiotic susceptibility and genome analysis of Enterococcus species isolated from inpatients in one hospital with no apparent outbreak of vancomycin-resistant Enterococcus in Japan
To prevent nosocomial infection, it is important to screen for potential vancomycin-resistant Enterococcus (VRE) among patients. In this study, we analyzed enterococcal isolates from inpatients in one hospital without any apparent outbreak of VRE. Enterococcal isolates were collected from inpatients at Hiroshima University Hospital from April 1 to June 30, 2021 using selective medium for Enterococci. Multilocus sequence typing, antimicrobial susceptibility testing, and whole-genome sequencing were performed. A total of 164 isolates, including Enterococcus faecium (41 isolates), Enterococcus faecalis (80 isolates), Enterococcus raffinosus (11 isolates), Enterococcus casseliflavus (nine isolates), Enterococcus avium (12 isolates), Enterococcus lactis (eight isolates), Enterococcus gallinarum (two isolates), and Enterococcus malodoratus (one isolate), were analyzed. We found one vanA-positive E. faecium, which was already informed when the patient was transferred to the hospital, nine vanC-positive E. casseliflavus, and two vanC-positive E. gallinarum. E. faecium isolates showed resistance to ampicillin (95.1%), imipenem (95.1%), and levofloxacin (87.8%), and E. faecalis isolates showed resistance to minocycline (49.4%). Ampicillin- and levofloxacin-resistant E. faecium had multiple mutations in penicillin-binding protein 5 (PBP5) (39/39 isolates) and ParC/GyrA (21/36 isolates), respectively. E. raffinosus showed resistance to ampicillin (81.8%), imipenem (45.5%), and levofloxacin (45.5%), and E. lactis showed resistance to ampicillin (37.5%) and imipenem (50.0%). The linezolid resistance genes optrA and cfr(B) were found only in one isolate of E. faecalis and E. raffinosus, respectively. This study, showing the status of enterococci infection in hospitalized patients, is one of the important information when considering nosocomial infection control of VRE.
期刊介绍：
Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses.
Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.
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