Microbiology and Immunology最新文献

A Mouse Model of Ovalbumin-Induced Airway Allergy Exhibits Altered Localization of SARS-CoV-2-Susceptible Cells in the Lungs, Which Reflects Omicron BA.5 Infection Dynamics, Viral Mutations, and Immunopathology. 卵清蛋白诱导的气道过敏小鼠模型显示出肺部 SARS-CoV-2 易感细胞定位的改变，这反映了 Omicron BA.5 感染动态、病毒突变和免疫病理学。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2024-11-21 DOI: 10.1111/1348-0421.13184

Takao Iketani, Kaya Miyazaki, Naoko Iwata-Yoshikawa, Yusuke Sakai, Nozomi Shiwa-Sudo, Seiya Ozono, Hideki Asanuma, Hideki Hasegawa, Tadaki Suzuki, Noriyo Nagata

{"title":"A Mouse Model of Ovalbumin-Induced Airway Allergy Exhibits Altered Localization of SARS-CoV-2-Susceptible Cells in the Lungs, Which Reflects Omicron BA.5 Infection Dynamics, Viral Mutations, and Immunopathology.","authors":"Takao Iketani, Kaya Miyazaki, Naoko Iwata-Yoshikawa, Yusuke Sakai, Nozomi Shiwa-Sudo, Seiya Ozono, Hideki Asanuma, Hideki Hasegawa, Tadaki Suzuki, Noriyo Nagata","doi":"10.1111/1348-0421.13184","DOIUrl":"https://doi.org/10.1111/1348-0421.13184","url":null,"abstract":"Asthma, an allergic disease of the airways, is a risk factor for severity of common respiratory viral infections; however, the relationship between asthma and severity in COVID-19 remains unclear. Here, we examined the effects of SARS-CoV-2 (Omicron BA.5 strain) infection in a mouse model of airway allergy. First, stimulation of allergic mice with OVA resulted in the appearance of ACE2-negative mucus-secreting goblet cells in the bronchiolar region, and an increase in the number of ACE2-expressing cells in the alveoli. As a result, ACE2-expressing cells, which are susceptible to SARS-CoV-2, were limited to the distal portion of the bronchioles while they increased in the alveolar area. After viral infection, the peak infectious viral load in the OVA group was 100-fold lower than that in the phosphate buffered saline (PBS) group; however, clearance of viral RNA from the upper/lower airways was delayed. There were notable differences in acquisition of nsp5 and nsp6 mutations by the Omicron BA.5 strain recovered from BALF samples obtained from the OVA and PBS groups. Immune responses associated with viral clearance were essentially the same, but expression of granulocyte-associated chemokines was higher, M2 macrophage responses were predominant, and the higher spike-specific IgG1/IgG2a ratio in the OVA group post-infection. Infection localized in the alveolar region earlier in the OVA group, resulting in more severe alveolar damage than in the PBS group. These data suggest a Th2-shifted immune background and altered localization of SARS-CoV-2 susceptible cells in mice with OVA-induced airway allergy, which reflect Omicron BA.5 infection dynamics, viral mutations, and immunopathology.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142686533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Characterization of a Novel Retron Element Isolated from Vibrio mimicus. 从拟态弧菌中分离出的一种新型 Retron 元素的遗传特征。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2024-11-18 DOI: 10.1111/1348-0421.13181

Jant Cres Caigoy, Toshi Shimamoto, Yojiro Ishida, Ashraf M Ahmed, Shin-Ichi Miyoshi, Tadashi Shimamoto

{"title":"Genetic Characterization of a Novel Retron Element Isolated from Vibrio mimicus.","authors":"Jant Cres Caigoy, Toshi Shimamoto, Yojiro Ishida, Ashraf M Ahmed, Shin-Ichi Miyoshi, Tadashi Shimamoto","doi":"10.1111/1348-0421.13181","DOIUrl":"10.1111/1348-0421.13181","url":null,"abstract":"Bacterial reverse transcriptase coding gene (RT) is essential for the production of a small satellite DNA-RNA complex called multicopy single-stranded DNA (msDNA). In this study, we found a novel retron, retron-Vmi1 (Vm85) from Vibrio mimicus. The retron is comprised of the msr-msd region, orf323, and the ret gene, a genetic organization similar to Salmonella's retron-Sen2 (St85). The protein sequence of the RNA-directed DNA polymerase (RT-Vmi1) is highly homologous to the RTs of Vibrio metoecus, Vibrio parahaemolyticus, and Vibrio vulnificus. Phylogenetic and protein sequence similarity analysis of retron-Vmi1 ORF323 and RT revealed a close relatedness to retron-Sen2. We found that retron-Vmi1 was inserted in the dusA gene, similar to the insertion of the retron-Vpa1 (Vp96) of V. parahaemolyticus AQ3354, suggesting that retrons can be transferred via the tRNA gene. These results are the first convincing evidence that retron is moving across species. The neighboring genes of retron-Vmi1 shared high homology with the genetic environment of V. parahaemolyticus and V. vulnificus retrons. We also found two junction points within the retron-Vmi1 and the dusA gene suggesting that retron-Vmi1 was inserted into this site in a two-step manner.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient Neutralizing Antibodies Induction by Human Parvovirus B19 Epitope-Presenting Protein Nanoparticles. 人类 Parvovirus B19 表位蛋白纳米颗粒诱导的高效中和抗体

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2024-11-15 DOI: 10.1111/1348-0421.13182

Sakika Kimura, Hidehiko Suzuki, Yu Hatakeyama, Takafumi Noguchi, Koga Ii, Kazumasa Nakamura, Hirotaka Ebina, Eiji Morita

{"title":"Efficient Neutralizing Antibodies Induction by Human Parvovirus B19 Epitope-Presenting Protein Nanoparticles.","authors":"Sakika Kimura, Hidehiko Suzuki, Yu Hatakeyama, Takafumi Noguchi, Koga Ii, Kazumasa Nakamura, Hirotaka Ebina, Eiji Morita","doi":"10.1111/1348-0421.13182","DOIUrl":"10.1111/1348-0421.13182","url":null,"abstract":"Human parvovirus B19 (B19V) causes fetal hydrops in pregnant women. Despite the significant impact of this virus, effective vaccines remain unclear. In this study, we successfully engineered B19V protein nanoparticles by fusing the N-terminal receptor-binding domain corresponding to 5-80 amino acids of VP1 with two distinct types of self-assembling protein nanoparticles. Gel filtration and electron microscopic analysis confirmed the spherical assembly of the antigen-fused nanoparticles. The purified nanoparticles are efficiently bound to the surface of UT7/Epo-S1 cells, which are semi-permissive hosts for B19V infection. Immunization of BALB/c mice with VP1u 5-80 nanoparticles elicited a robust production of B19V-specific IgG antibodies compared to single VP1u 5-80 peptides. Moreover, a neutralization assay using B19V derived from a blood donor sample revealed that antibodies from mice immunized with VP1u 5-80 nanoparticles exhibited stronger infection-neutralizing activity. These findings suggest that nanoparticle formation plays a crucial role in enhancing the immunogenicity of the B19V VP1u 5-80 amino acid peptide and that these nanoparticles could serve as promising vaccine candidates, effectively inducing immunity against B19V.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lack of Evidence for Transmission of Atypical H-Type Bovine Spongiform Encephalopathy Prions (H-BSE Prions) by Intracranial and Oral Challenges to Nonhuman Primates. 缺乏证据证明非人灵长类动物通过颅内和口服挑战传播非典型 H 型牛海绵状脑病朊病毒 (H-BSE Prions)。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2024-11-11 DOI: 10.1111/1348-0421.13180

Hiroaki Shibata, Fumiko Ono, Yuko Sato, Keiko Ohto, Nozomi Nakano, Morikazu Imamura, Motohiro Horiuchi, Minoru Tobiume, Ken'ichi Hagiwara

{"title":"Lack of Evidence for Transmission of Atypical H-Type Bovine Spongiform Encephalopathy Prions (H-BSE Prions) by Intracranial and Oral Challenges to Nonhuman Primates.","authors":"Hiroaki Shibata, Fumiko Ono, Yuko Sato, Keiko Ohto, Nozomi Nakano, Morikazu Imamura, Motohiro Horiuchi, Minoru Tobiume, Ken'ichi Hagiwara","doi":"10.1111/1348-0421.13180","DOIUrl":"https://doi.org/10.1111/1348-0421.13180","url":null,"abstract":"Bovine spongiform encephalopathy (BSE) is a prion disease in cattle caused by classical-type (C-), L-type (L-), or H-type (H-) BSE prions. While C-BSE prions are zoonotic agents responsible for variant Creutzfeldt-Jakob disease, L- and H-BSE prions are believed not to be connected to human prion diseases. However, L-BSE prions have been shown to transmit to cynomolgus monkeys (Macaca fascicularis), suggesting they may have zoonotic potential. In the present study, we examined whether H-BSE prions are transmissible to cynomolgus monkeys. The monkeys were injected intracranially (n = 2) or given orally (n = 2) with brain homogenates from a cow infected with H-BSE prions. After asymptomatic observation periods of 4-6 years, the monkeys were euthanized for autopsy. Histological examination of the brain did not reveal any pathological changes. Immunohistochemical and Western blot analyses did not detect disease-associated forms of prion protein (PrPSc) in the brain, peripheral neurons, or lymphatic tissues. The unsuccessful transmission indicates an effective barrier against the transmission of cattle H-BSE prions to cynomolgus monkeys. Based on the results obtained in this nonhuman primate model, we estimated that the potential transmission of H-BSE prions to humans is substantially lower than C- and L-BSE prions.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of an Inactivated Whole-Virus A/Victoria/361/2011 (IVR-165) (H3N2) Influenza Vaccine in Ferrets. 灭活整病毒 A/Victoria/361/2011 (IVR-165) (H3N2) 流感疫苗在雪貂中的疗效。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2024-11-08 DOI: 10.1111/1348-0421.13179

Noriko Kishida, Masaki Imai, Akira Ainai, Hideki Asanuma, Reiko Saito, Seiichiro Fujisaki, Masayuki Shirakura, Kazuya Nakamura, Tomoko Kuwahara, Emi Takashita, Masato Tashiro, Takato Odagiri, Shinji Watanabe

{"title":"Efficacy of an Inactivated Whole-Virus A/Victoria/361/2011 (IVR-165) (H3N2) Influenza Vaccine in Ferrets.","authors":"Noriko Kishida, Masaki Imai, Akira Ainai, Hideki Asanuma, Reiko Saito, Seiichiro Fujisaki, Masayuki Shirakura, Kazuya Nakamura, Tomoko Kuwahara, Emi Takashita, Masato Tashiro, Takato Odagiri, Shinji Watanabe","doi":"10.1111/1348-0421.13179","DOIUrl":"https://doi.org/10.1111/1348-0421.13179","url":null,"abstract":"It has been reported that the high-growth reassortant (HGR) A(H3N2) influenza viruses used for split influenza vaccine (SV) production have some amino acid substitutions in hemagglutinin due to egg adaptation during virus propagation, causing antigenic differences between HGR and epidemic viruses. To clarify whether inactivated whole-virus vaccine (WV) derived from the A(H3N2) HGR virus possessing egg adaptation could induce cross-protective immune responses against epidemic A(H3N2) viruses, the efficacy of WV was compared with that of SV in a ferret model. When the ferrets immunized with WV or SV derived from HGR A/Victoria/361/2011 (IVR-165) virus were challenged with the homologous virus A/Victoria/361/2011 (IVR-165) or its original cell-propagated A/Victoria/361/2011 virus, respectively, WV successfully shortened the duration of virus shedding of both challenge viruses, whereas SV shortened only that of the homologous virus, A/Victoria/361/2011 (IVR-165). When WV-immunized ferrets were challenged with A/Fukushima/69/2015 virus, which is an epidemic virus antigenically different from the A/Victoria/361/2011 virus, WV could shorten the duration of shedding of this virus. In addition, we found that early induction of nasal IgG and IgA antibodies by vaccines helped shorten the virus-shedding period, although this was dependent on the degree of difference in antigenicity of the challenge virus. These results indicate that vaccination with WV, not with SV, would be a solution to avoid decreased vaccine effectiveness due to the antigenic change of HGR virus by egg adaptation during virus propagation.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information – Cover 发行信息 - 封面

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2024-11-06 DOI: 10.1111/1348-0421.13177

引用次数: 0

Structure-based virtual screening and drug repurposing studies indicate potential inhibitors of bovine papillomavirus E6 oncoprotein. 基于结构的虚拟筛选和药物再利用研究揭示了牛乳头瘤病毒 E6 肿瘤蛋白的潜在抑制剂。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2024-10-28 DOI: 10.1111/1348-0421.13178

Lucas Alexandre Barbosa de Oliveira Santos, Marcus Vinicius de Aragão Batista

引用次数: 0

Downregulation of CD86 in HCMV-infected THP-1 cells. 下调 HCMV 感染的 THP-1 细胞中的 CD86。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2024-10-09 DOI: 10.1111/1348-0421.13176

Tetsuo Koshizuka, Yuta Sasaki, Hiroki Kondo, Juri Koizumi, Keita Takahashi

{"title":"Downregulation of CD86 in HCMV-infected THP-1 cells.","authors":"Tetsuo Koshizuka, Yuta Sasaki, Hiroki Kondo, Juri Koizumi, Keita Takahashi","doi":"10.1111/1348-0421.13176","DOIUrl":"https://doi.org/10.1111/1348-0421.13176","url":null,"abstract":"Monocytes and macrophages are at the frontline of defense against pathogens. Human cytomegalovirus (HCMV) uses myeloid cells as vehicles to facilitate viral dissemination. HCMV infection in monocytes and macrophages leads to the downregulation of several cell surface markers via an undefined mechanism. Previously, we showed that HCMV pUL42 associates with the Nedd4 family ubiquitin E3 ligases through the PPXY motif on pUL42 and downregulates Nedd4 and Itch proteins in HCMV-infected fibroblasts. Homologous proteins of HCMV pUL42, such as HHV-6 U24, downregulate cell surface markers. To reveal the downregulation property of pUL42, we focused on CD86, the key costimulatory factor for acquired immunity. Here, we constructed CD86-expressing THP-1 cells using a retroviral vector and analyzed the effects of HCMV infection and pUL42 on CD86 downregulation. Disruption of the PPXY motifs of pUL42 (UL42PA) decelerated the degradation of CD86 in recombinant virus-infected cells, indicating the involvement of Nedd4 family functions. However, no direct interactions were observed between CD86 and Itch. Interestingly, unlike fibroblast infection, the expression of Nedd4 and Itch proteins increased in HCMV-infected THP-1 cells, accompanied by an increase in their transcript levels. Although the function of pUL42 did not relate to the increase of Nedd4 and Itch proteins, pUL42 should affect these Nedd4 proteins to downregulate CD86.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142391758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information – Cover 发行信息 - 封面

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2024-10-05 DOI: 10.1111/1348-0421.13174

引用次数: 0

First detection and biological characterization of an avian metaavulavirus 8 isolated from a migratory swan goose in Qinghai Lake, Northwest China. 首次从中国西北地区青海湖迁徙天鹅中分离出禽类元病毒 8，并对其进行生物学鉴定。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2024-10-03 DOI: 10.1111/1348-0421.13175

Sijie Wang, Xinxin Liu, Jianjun Chen, Weiwen Yan, Hongjin Li, Weiwei Chi, Rui Luo, Xianwen Lin, Yue Yin, Chuanrong Dong, Huihui Wang, Bowen Zheng, Hongli Li, Yifei Liu, Tobias Stoeger, Abdul Wajid, Aleksandar Dodovski, Chao Gao, Claro N Mingala, Dmitry B Andreychuk, Renfu Yin

{"title":"First detection and biological characterization of an avian metaavulavirus 8 isolated from a migratory swan goose in Qinghai Lake, Northwest China.","authors":"Sijie Wang, Xinxin Liu, Jianjun Chen, Weiwen Yan, Hongjin Li, Weiwei Chi, Rui Luo, Xianwen Lin, Yue Yin, Chuanrong Dong, Huihui Wang, Bowen Zheng, Hongli Li, Yifei Liu, Tobias Stoeger, Abdul Wajid, Aleksandar Dodovski, Chao Gao, Claro N Mingala, Dmitry B Andreychuk, Renfu Yin","doi":"10.1111/1348-0421.13175","DOIUrl":"https://doi.org/10.1111/1348-0421.13175","url":null,"abstract":"Avian metaavulavirus 8 (AMAV-8), formerly known as avian paramyxovirus 8 (APMV-8), has been detected sporadically in wild birds worldwide since it was first identified in a Canadian goose in 1976. However, the presence of AMAV-8 in birds has never been reported in China. To understand the epidemiological situation of AMAV-8 and its ability to infect chickens, we conducted a surveillance study and in vivo analysis of the AMAV-8 isolate identified in total of 14,909 clinical samples collected from wild and domestic birds from 2014 to 2022 in China. However, in 2017, only one AMAV-8 virus (Y7) was successful isolated from the fresh droppings of a migratory swan goose in Qinghai Lake in Northwest China. Thereafter, we report the complete genome sequence of the Y7 strain with a genome length of 15,342 nucleotides and the Y7 isolate was genetically closely-related to wild bird-origin AMAV-8 viruses previously circulated in the United States, Japan, and Kazakhstan. Furthermore, AMAV-8 infections of one-day-old specific pathogen-free (SPF) chicks did not induce any clinical signs over the entire observation period but was associated with viral shedding for up to 8 days. Interestingly, although all birds infected with the Y7 strain seroconverted within the first week of infection, virus replication was only detected in the trachea but not in other tissues such as the brain, lung, or heart. Here, we report the complete genome, genetic and biological characterization, replication and pathogenicity analysis in vivo and first detection of AMAV-8 in China.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142365793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0