Microbiology and Immunology最新文献_第10页

Emergence of Vibrio parahaemolyticus serotype O10:K4 in Thailand 泰国出现血清型O10:K4副溶血性弧菌

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-01-23 DOI: 10.1111/1348-0421.13055

Kazuhisa Okada, Amonrattana Roobthaisong, Suthida Muangnoicharoen Hearn, Pilailuk Akkapaiboon Okada, Pawinee Doung-Ngern, Warawan Wongboot, Atchareeya Nakkarach, Masatomo Morita, Toshio Kodama, Tetsuya Iida

引用次数: 2

A novel toxin–antitoxin system swpAB alters gene expression patterns and reduces virulence expression in enterohemorrhagic Escherichia coli 一种新的毒素-抗毒素系统swpAB改变肠出血性大肠杆菌的基因表达模式并降低毒力表达

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-01-12 DOI: 10.1111/1348-0421.13054

Shinya Ebihara, Hilo Yen, Toru Tobe

{"title":"A novel toxin–antitoxin system swpAB alters gene expression patterns and reduces virulence expression in enterohemorrhagic Escherichia coli","authors":"Shinya Ebihara, Hilo Yen, Toru Tobe","doi":"10.1111/1348-0421.13054","DOIUrl":"10.1111/1348-0421.13054","url":null,"abstract":"Toxin–antitoxin (TA) systems are found widely among many bacteria, including enterohemorrhagic Escherichia coli (EHEC), but their functions are still poorly understood. In this study, we identified and characterized a novel TA system belonging to the relBE family, classified as a type II TA system, found in EHEC. The protein encoded by the toxin gene is homologous to RelE ribonuclease. Using various conditions for increasing the toxin activity, high-level induction of a toxin gene, and repression of an antitoxin gene in wild-type EHEC, we showed that the TA system, named swpAB (switching of gene expression profile), is involved in selective repression of a set of genes, including some virulence genes, and in the reduction of adherence capacity, rather than in suppression of bacterial growth. A detailed analysis of the profiles of RNA levels along sequences at 15 min after high expression of swpA revealed that two virulence genes, espA and tir, were direct targets of the SwpA toxin. These results suggested that the swpAB system can alter gene expression patterns and change bacterial physiological activity without affecting bacterial growth.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9255267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Avian paramyxovirus serotype-1 isolation from migratory birds and environmental water in southern Japan: An epidemiological survey during the 2018/19–2021/2022 winter seasons 日本南部候鸟和环境水体分离禽副粘病毒血清型1:2018/19-2021/2022冬季流行病学调查

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-01-10 DOI: 10.1111/1348-0421.13053

Nonoka Matsunaga, Moe Ijiri, Kemi Ishikawa, Makoto Ozawa, Kosuke Okuya, Ahmed Magdy Khalil, Isshu Kojima, Mana Esaki, Tatsunori Masatani, Tsutomu Matsui, Yoshikazu Fujimoto

{"title":"Avian paramyxovirus serotype-1 isolation from migratory birds and environmental water in southern Japan: An epidemiological survey during the 2018/19–2021/2022 winter seasons","authors":"Nonoka Matsunaga, Moe Ijiri, Kemi Ishikawa, Makoto Ozawa, Kosuke Okuya, Ahmed Magdy Khalil, Isshu Kojima, Mana Esaki, Tatsunori Masatani, Tsutomu Matsui, Yoshikazu Fujimoto","doi":"10.1111/1348-0421.13053","DOIUrl":"10.1111/1348-0421.13053","url":null,"abstract":"Newcastle disease caused by highly pathogenic viruses of avian paramyxovirus serotype-1 (APMV-1) is a highly contagious poultry disease. Although a large-scale epidemic of Newcastle disease had occurred in Japan between the 1950s and the 2000s, there have been no outbreaks anywhere since 2010. In addition, there are no reports of epidemiological surveys of APMV-1 in wild birds in Japan in the last 10 years. We conducted the first epidemiological survey of APMV-1 in the Izumi plain, Kagoshima prefecture of southern Japan from the winter of 2018 to 2022. A total of 15 APMV-1 strains were isolated, and isolation rates from roosting water and duck fecal samples were 2.51% and 0.10%, respectively. These results indicate that the isolation method from environmental water may be useful for efficient surveillance of APMV-1 in wild birds. Furthermore, this is the first report on the success of APMV-1 isolation from environmental water samples. Genetic analysis of the Fusion (F) gene showed that all APMV-1 isolates were closely related to virus strains circulating among waterfowl in Far East Asian countries. All isolates have avirulent motifs in their cleavage site of F genes, all of which were presumed to be low pathogenic viruses in poultry. However, pathogenicity test using embryonated chicken eggs demonstrated that some isolates killed all chicken embryos regardless of viral doses inoculated (102–106 50% egg infectious dose). These results indicated that APMV-1 strains, which are potentially pathogenic to chickens, are continuously brought into the Izumi plain by migrating wild birds.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9623938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation of a photocontrollable recombinant bovine parainfluenza virus type 3 光可控重组牛副流感病毒3型的产生

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-01-06 DOI: 10.1111/1348-0421.13052

Takashi Okura, Maino Tahara, Noriyuki Otsuki, Moritoshi Sato, Kaoru Takeuchi, Makoto Takeda

引用次数: 0

Issue Information – Cover 问题信息-封面

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-01-06 DOI: 10.1111/1348-0421.12987

引用次数: 0

Establishment of a system to quantify wild-type herpes simplex virus–induced cell–cell fusion reveals a role of N-glycosylation of HSV-1 envelope glycoprotein B in cell–cell fusion 野生型单纯疱疹病毒诱导的细胞-细胞融合系统的建立揭示了HSV-1包膜糖蛋白B n -糖基化在细胞-细胞融合中的作用

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-01-06 DOI: 10.1111/1348-0421.13050

Ayano Fukui, Yuhei Maruzuru, Kosuke Takeshima, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi

引用次数: 2

Characterization of missense mutations in the NADPH oxidase partner p22phox in the A22° subtype of chronic granulomatous disease 慢性肉芽肿性疾病A22°亚型NADPH氧化酶伴侣p22phox错义突变的特征

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-01-06 DOI: 10.1111/1348-0421.13051

Chikage Kawai, Mizuho Kajikawa, Akira Yamauchi, Shuichiro Okamoto, Futoshi Kuribayashi, Kei Miyano

{"title":"Characterization of missense mutations in the NADPH oxidase partner p22phox in the A22° subtype of chronic granulomatous disease","authors":"Chikage Kawai, Mizuho Kajikawa, Akira Yamauchi, Shuichiro Okamoto, Futoshi Kuribayashi, Kei Miyano","doi":"10.1111/1348-0421.13051","DOIUrl":"https://doi.org/10.1111/1348-0421.13051","url":null,"abstract":"Defective superoxide production by NADPH oxidase 2 (Nox2) in phagocyte cells results in the development of chronic granulomatous disease (CGD), a hereditary disease characterized by recurrent and life-threatening infections. The partner protein p22phox is a membrane-spanning protein which forms a stable heterodimer with Nox2 in the endoplasmic reticulum. This interaction ensures the stability of each protein and their accurate trafficking to the cell membrane. The present paper describes the characterization of p22phox missense mutations that were identified in a patient with CGD who presented with undetectable levels of p22phox. Using a reconstitution system, it was found that p22phox expression decreased when R90Q, A117E, S118R, A124S, A124V, A125T, or E129K mutations were introduced, suggesting that these mutations destabilize the protein. In contrast, introducing an L105R mutation did not affect protein expression, but did inhibit p22phox binding to Nox2. Thus, the missense mutations discussed here contribute to the development of CGD by either disrupting protein stability or by impairing the interaction between p22phox and Nox2.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50122816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The roles of BST-2 in murine B cell development and on virus propagation BST-2在小鼠B细胞发育和病毒繁殖中的作用

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-01-05 DOI: 10.1111/1348-0421.13049

Shuzo Urata, Sachiko Yamaguchi, Aya Nambu, Katsuko Sudo, Susumu Nakae, Jiro Yasuda

{"title":"The roles of BST-2 in murine B cell development and on virus propagation","authors":"Shuzo Urata, Sachiko Yamaguchi, Aya Nambu, Katsuko Sudo, Susumu Nakae, Jiro Yasuda","doi":"10.1111/1348-0421.13049","DOIUrl":"10.1111/1348-0421.13049","url":null,"abstract":"The bone marrow (BM) stromal cell antigen-2 (BST-2), also known as tetherin, CD317, PDCA-1, or HM1.24, is a membrane protein overexpressed in several types of tumors and may act as a promising target for cancer treatment via antibody-dependent cellular cytotoxicity. BST-2 is also expressed in human BM stromal cells (BMSC), which support B cell development. While the activity of BST-2 as an antiviral factor has been demonstrated, the expression patterns and the role of BST-2 on B-cell development and activation have not been investigated, especially in vivo. In this study, Bst2 knockout (Bst2−/−) mice were generated to assess the role of BST-2 on B cell development and activation. It was observed that BST-2 was not expressed in BMSC or all B cell progenitors even in wild-type mice and does not play a significant role in B cell development. In addition, the loss of BST-2 had no effect on B cell activation. Furthermore and in contrast to the well-known antiviral role of BST-2, infection of vesicular stomatitis Indiana virus to the BM cells collected from the Bst2−/− mice produced less infectious virus compared with that from the WT mice. These results suggest that murine BST-2 is different from human BST-2 in the expression pattern, physiological function, in vivo, and might possess positive role on VSV replication.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2023-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9080422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

West Nile virus–associated HLA-DRB1 alleles in the Greek population: A structural perspective 希腊人群中西尼罗病毒相关HLA-DRB1等位基因:结构视角

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2022-12-28 DOI: 10.1111/1348-0421.13048

Constantina A. Sarri, Georgios E. Papadopoulos, Zissis Mamuris

{"title":"West Nile virus–associated HLA-DRB1 alleles in the Greek population: A structural perspective","authors":"Constantina A. Sarri, Georgios E. Papadopoulos, Zissis Mamuris","doi":"10.1111/1348-0421.13048","DOIUrl":"10.1111/1348-0421.13048","url":null,"abstract":"The HLA system plays a significant role via the regulation of the immune system and contributes to the progression and protection of many diseases. In our previous study, several HLA-DRB1 alleles were found to have a susceptible or protective role toward infection and neuroinvasion of West Nile Virus (WNV) in the Greek population. As expected, the majority of polymorphic positions are located in the peptide-binding region of the molecule. In the present work, the structure of these alleles was studied in silico, to examine the effect of polymorphism on the conformation of DRB1 proteins, with the aspect of WNV association. More specifically, molecular dynamics simulations were used for structural prediction of 23 available alleles. These modeled alleles were evaluated using root-mean-square deviation (RMSD) and root-mean-square fluctuation analysis. Low RMSD values indicate that different alleles have similar structures. Furthermore, low fluctuation was observed in the peptide-binding region between alleles with the higher and the lowest RMSD values. These findings indicate that probably variable residues do not affect the behavior of DRB1 alleles in WNV disease, by causing structural differences between them.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.13048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9391323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of measles virus receptor-expressing Vero cells lacking functional poliovirus receptors 缺乏功能性脊髓灰质炎病毒受体的麻疹病毒受体表达Vero细胞的建立

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2022-12-23 DOI: 10.1111/1348-0421.13047

Kenji Someya, Yuko Okemoto-Nakamura, Takako Kurata, Daiki Kanbayashi, Noriko Saito, Masae Itamochi, Noriyuki Otsuki, Kentaro Hanada, Makoto Takeda

引用次数: 0