Microbiology and Immunology最新文献

{"title":"Issue Information – Cover","authors":"","doi":"10.1111/1348-0421.13008","DOIUrl":"https://doi.org/10.1111/1348-0421.13008","url":null,"abstract":"<p><b>Cover photograph</b>: Confocal fluorescence pictures of cultured macrophages stained using different clones of CD169 (blue: DAPI; green: HSn7D2; red: SP216). <i>Microbiol Immunol: 67:490–500</i>. Article link here\u0000 \u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 11","pages":"i-ii"},"PeriodicalIF":2.6,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.13008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137811150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characterization of New Delhi metallo-beta-lactamase–producing species of Morganellaceae, Yersiniaceae, and Enterobacteriaceae (other than Klebsiella) from Brazil over 2013–2022","authors":"Carlos Henrique Camargo,&nbsp;Amanda Yaeko Yamada,&nbsp;Andreia Rodrigues de Souza,&nbsp;Claudio Tavares Sacchi,&nbsp;Alex Domingos Reis,&nbsp;Marlon Benedito Nascimento Santos,&nbsp;Denise Brandão de Assis,&nbsp;Eneas de Carvalho,&nbsp;Elizabeth Harummyy Takagi,&nbsp;Marcos Paulo Vieira Cunha,&nbsp;Monique Ribeiro Tiba-Casas","doi":"10.1111/1348-0421.13100","DOIUrl":"10.1111/1348-0421.13100","url":null,"abstract":"<p>Over the last decade, New Delhi metallo-beta-lactamase (NDM) carbapenemase has silently spread in Brazil. In this study, we analyzed a large collection of Enterobacterales other than <i>Klebsiella</i> spp. received in our reference laboratory between 2013 and 2022. A total of 32 clinical isolates displaying different pulsed-field gel electrophoresis profiles, and represented by 11 species in the families Enterobacteriaceae (<i>Citrobacter freundii</i>, <i>Citrobacter portucalensis</i>, <i>Enterobacter hormaechei</i>, and <i>Escherichia coli</i>), Morganellaceae (<i>Morganella morganii</i>, <i>Proteus mirabilis</i>, <i>Proteus vulgaris</i>, <i>Providencia rettgeri</i>, <i>Providencia stuartii</i>, and <i>Raoultella ornithinolytica</i>), and Yersiniaceae (<i>Serratia marcescens</i>) had their whole genomes sequenced and further analyzed. Antimicrobial susceptibility was determined by disk diffusion, except for polymyxin B, assessed by broth microdilution. The <i>bla</i>_NDM-1 allele was predominant (<i>n</i> = 29), but <i>bla</i>_NDM-5 was identified in an <i>E. coli</i> specimen with a novel ST, and the <i>bla</i>_NDM-7 allele was found in <i>E. hormaechei</i> ST45 and <i>E. coli</i> ST1049. Polymyxin was active against all but one Enterobacteriaceae isolate: an <i>mcr-1</i>–producing <i>E. coli</i> presenting minimal inhibitory concentration (4 mg/L). Isolates producing extended-spectrum β-lactamases were common: cefotaximase from Munich (CTX-M)-15 (<i>n</i> = 10), CTX-M-2 (<i>n</i> = 4), and CTX-M-8 (<i>n</i> = 3) were detected, and the <i>mcr-1</i>–producing <i>E. coli</i> was found to co-produce both CTX-M-8 and CTX-M-55 β-lactamases. The <i>mcr-9</i> gene was found in 5/8 <i>E. hormaechei</i> isolates, distributed in four different sequence types, all of them presenting susceptibility to polymyxin. This study showed that NDM-producing Enterobacterales other than <i>Klebsiella</i> are already spread in Brazil, in diversified species, and cocarrying important resistance genes. Prompt detection and effective implementation of measures to prevent further spread are mandatory for mitigating the dissemination of NDM carbapenemase in hospital settings and preserving the already limited antimicrobial therapy options.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"68 1","pages":"1-5"},"PeriodicalIF":2.6,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49679658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natto extract inhibits infection caused by the Aujeszky's disease virus in mice","authors":"Junya Kobayashi,&nbsp;Rongduo Wen,&nbsp;Takanobu Nishikawa,&nbsp;Yuka Nunomura,&nbsp;Takehito Suzuki,&nbsp;Yudai Sejima,&nbsp;Toshiya Gokan,&nbsp;Makio Furukawa,&nbsp;Tomoko Yokota,&nbsp;Nanako Osawa,&nbsp;Yoko Sato,&nbsp;Yutaka Nibu,&nbsp;Tetsuya Mizutani,&nbsp;Mami Oba","doi":"10.1111/1348-0421.13099","DOIUrl":"10.1111/1348-0421.13099","url":null,"abstract":"<p>Aujeszky's disease virus (ADV), also known as Suid alphaherpesvirus 1, which mainly infects swine, causes life-threatening neurological disorders. This disease is a serious global risk factor for economic losses in the swine industry. The development of new anti-ADV drugs is highly anticipated and required. Natto, a traditional Japanese fermented food made from soybeans, is a well-known health food. In our previous study, we confirmed that natto has the potential to inhibit viral infections by severe acute respiratory syndrome coronavirus 2 and bovine alphaherpesvirus 1 through their putative serine protease(s). In this study, we found that an agent(s) in natto functionally impaired ADV infection in cell culture assays. In addition, ADV treated with natto extract lost viral infectivity in the mice. We conducted an HPLC gel-filtration analysis of natto extract and molecular weight markers and confirmed that Fraction No. 10 had ADV-inactivating ability. Furthermore, the antiviral activity of Fraction No. 10 was inhibited by the serine protease inhibitor 4-(2-Aminoethyl) benzene sulfonyl fluoride hydrochloride (AEBSF). These results also suggest that Fraction No. 10, adjacent to the 12.5 kDa peak of the marker in natto extract, may inactivate ADV by proteolysis. Our findings provide new avenues of research for the prevention of Aujeszky's disease.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 12","pages":"514-519"},"PeriodicalIF":2.6,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41183079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatic investigation of Nipah virus surface protein mutations: Molecular docking with Ephrin B2 receptor, molecular dynamics simulation, and structural impact analysis","authors":"Emre Aktaş,&nbsp;İrem Saygılı,&nbsp;Elif Kahveci,&nbsp;Zeynep Tekbıyık,&nbsp;Nehir Özdemir Özgentürk","doi":"10.1111/1348-0421.13098","DOIUrl":"10.1111/1348-0421.13098","url":null,"abstract":"The SARS‐CoV‐2 outbreak resulted in significant challenges and loss of life. The Nipah virus, known for its high infectivity and severity, was designated an emergency concern by the World Health Organization. To understand its mutations, the Nipah virus proteins were analyzed extensively, with a focus on the essential G and F proteins responsible for viral entry into host cells. Our bioinformatics analysis unveiled multiple mutations, including simultaneous mutations within a single sequence. Notably, the G273S mutation in the F protein was identified as a potential cause of structural damage, which carries significant implications for vaccine development. Comparing the docking scores of G and F proteins with the Ephrin B2 receptor, it was found that the Y228H mutation in the G protein and the D252G mutation in the F protein likely affect virus entry into host cells. Moreover, our investigation into stability and deformability highlighted the impact of the Y228H mutation in the G protein complex. Molecular dynamics simulations revealed increased flexibility and conformational changes in the G protein complex with the Y228H mutation compared with the known complex. Furthermore, evaluating the root mean square deviation variation demonstrated greater dynamic behavior in the G protein complex and the Ephrin B2 receptor complex. This comprehensive study provides valuable insights into Nipah virus mutations, their significance for vaccine development, and the importance of understanding protein complex behavior in drug discovery. The identified mutations, especially G273S and Y228H, hold crucial implications for future research and potential interventions against the Nipah virus.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 12","pages":"501-513"},"PeriodicalIF":2.6,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41163899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information – Cover","authors":"","doi":"10.1111/1348-0421.13005","DOIUrl":"https://doi.org/10.1111/1348-0421.13005","url":null,"abstract":"<p><b>Cover photograph</b>: Inflammasome activation by Listeria monocytogenes infection. <i>Microbiol Immunol: 67:429–437</i>. Article link here\u0000 \u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 10","pages":"i-ii"},"PeriodicalIF":2.6,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.13005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50131505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Siderophore-producing Pantoea ferrattrahens sp. nov. isolated from a clinical specimen and Pantoea ferramans sp. nov. isolated from soil at the bottom of a pond","authors":"Ryo Kutsuna,&nbsp;Tohru Miyoshi-Akiyama,&nbsp;Yuki Muramatsu,&nbsp;Moriyuki Hamada,&nbsp;Junko Tomida,&nbsp;Ken Kikuchi,&nbsp;Yoshiaki Kawamura","doi":"10.1111/1348-0421.13097","DOIUrl":"10.1111/1348-0421.13097","url":null,"abstract":"<p>Two Gram-negative facultative anaerobes were isolated from a sepsis patient with pancreatic cancer (strain PAGU 2156^T) and soil at the bottom of a pond (strain PAGU 2198^T), respectively. These two strains formed haloes around the colonies on chrome azurol S agar plates, indicating the production of siderophores. Two isolates assigned to the genus <i>Pantoea</i> based on the 16S rRNA gene were differentiated from established species by using polymorphic taxonomies. Phylogenetic analysis using four housekeeping genes (<i>gyrB, rpoB, atpD</i>, and <i>infB</i>) showed that strain PAGU 2156^T is closely related to <i>Pantoea cypripedii</i> LMG 2657^T (89.9%) or <i>Pantoea septica</i> LMG 5345^T (95.7%). Meanwhile, strain PAGU 2198^T formed a single clade with <i>Pantoea rodasii</i> DSM 26611^T (93.6%) and <i>Pantoea rwandensis</i> DSM 105076^T (93.3%). The average nucleotide identity values obtained from the draft genome assembly showed ≤90.2% between strain PAGU 2156^T and closely related species and ≤81.5% between strain PAGU 2198^T and closely related species. Based on various phenotypes, biochemical properties, and whole-cell fatty acid composition compared with related species, it was concluded that each strain should be classified as a new species of the genus <i>Pantoea</i>. In this manuscript, <i>Pantoea ferrattrahens</i> sp. nov. and <i>Pantoea ferramans</i> sp. nov. with strain PAGU 2156^T (=NBRC 115930^T = CCUG 76757^T) and strain PAGU 2198^T (=NBRC 114265^T = CCUG 75151^T) are proposed as each type strain.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 11","pages":"480-489"},"PeriodicalIF":2.6,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41123292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information – Cover","authors":"","doi":"10.1111/1348-0421.13003","DOIUrl":"https://doi.org/10.1111/1348-0421.13003","url":null,"abstract":"<p><b>Cover photograph</b>: Circular map of the genome of Acinetobacter baumannii CYZ. <i>Microbiol Immunol: 67:396–403</i>. Article link here\u0000 \u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 9","pages":"i-ii"},"PeriodicalIF":2.6,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.13003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50115897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of macrophage/dendritic cell–related molecules in lymph node sinus macrophages","authors":"Rin Yamada,&nbsp;Koji Ohnishi,&nbsp;Cheng Pan,&nbsp;Hiromu Yano,&nbsp;Yukio Fujiwara,&nbsp;Takuya Shiota,&nbsp;Yoshiki Mikami,&nbsp;Yoshihiro Komohara","doi":"10.1111/1348-0421.13095","DOIUrl":"10.1111/1348-0421.13095","url":null,"abstract":"<p>The role of sinus macrophages (SMs) in anticancer immune responses has received considerable interest in recent years, but the types of molecules that are expressed in human SMs have not yet been clarified in detail. We therefore sought to identify dendritic cell (DC)– or macrophage-related molecules in SMs in human lymph nodes (LNs). SMs are strongly positive for Iba-1, CD163, CD169, and CD209. CD169 (clone SP216) reacted with almost all SMs, mainly in the cell surface membrane, while CD169 (clone HSn 7D2) reacted with a subpopulation of SMs, mainly in the cytoplasm, with a significant increase observed after IFN-α stimulation. The immunoreactivity of clone HSn 7D2 was markedly reduced after transfection with small interfering RNA against CD169, while that of clone SP216 was slightly reduced. The induction of <i>CCL8</i> and <i>CXCL10</i> messenger RNA (mRNA) expression by IFN-α was confirmed using cultured macrophages and RT-qPCR, but fluorescence <i>in situ</i> hybridization did not detect <i>CCL8</i> and <i>CXCL10</i> mRNA expression in SMs. Single-cell RNA sequence data of LNs indicated that the highest level of <i>CXCL10</i> gene expression occurred in monocytes. In conclusion, we found that CD209, also known as DC-related molecule, was expressed in human SMs. The heterogeneity observed in CD169 reacted with cone HSn 7D2 and SP216 was potentially due to the modification of CD169 protein by IFN stimulation. Further, no expression of <i>CXCL10</i> mRNA in SMs suggested that SMs might be resident macrophages.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 11","pages":"490-500"},"PeriodicalIF":2.6,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10069381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TLR2 mediates autophagy through ERK signaling pathway in Chlamydia psittaci CPSIT_p7 protein-stimulated RAW264.7 cells","authors":"Ying Luo,&nbsp;Zhenjie Sun,&nbsp;Qian Chen,&nbsp;Jian Xiao,&nbsp;XiaoLiang Yan,&nbsp;Yumeng Li,&nbsp;Yimou Wu","doi":"10.1111/1348-0421.13096","DOIUrl":"10.1111/1348-0421.13096","url":null,"abstract":"<p><i>Chlamydia psittaci</i> is a zoonotic pathogen found in birds and humans. Macrophages, major components of the innate immune system, can resist chlamydial infections and trigger adaptive immune responses. However, the molecular mechanisms underlying the action of macrophages against <i>C. psittaci</i> infection are not well understood. This study investigated the roles and mechanisms of plasmid-encoded protein CPSIT_p7 of <i>C. psittaci</i> in regulating autophagy in RAW264.7 cells. The results demonstrated that stimulation of RAW264.7 with <i>C. psittaci</i> plasmid protein CPSIT_p7 induced the expressions of the autophagy signaling primary regulators LC3 and Beclin1, which could also significantly induce the phosphorylation levels of ERK, JNK, p38, and Akt. Next, siRNA knockdown of TLR2 resulted in significant downregulation of CPSIT_p7-triggered autophagy in RAW264.7 cells. Moreover, the extracellular regulated protein kinase (ERK) inhibitor PD98059 markedly reduced autophagy in CPSIT_p7-stimulated macrophages. In summary, these results indicated that TLR2 plays an essential role in the induction of autophagy through the ERK signaling pathway in CPSIT_p7-stimulated RAW264.7 cells.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 11","pages":"469-479"},"PeriodicalIF":2.6,"publicationDate":"2023-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10416046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multilocus sequence typing method of Staphylococcus aureus DNAs in a sample from human skin","authors":"Hiroka Furuya,&nbsp;Kohei Ogura,&nbsp;Norihiko Takemoto,&nbsp;Shinya Watanabe,&nbsp;Ayaka Yamazaki,&nbsp;Kazuhiro Ogai,&nbsp;Junko Sugama,&nbsp;Shigefumi Okamoto","doi":"10.1111/1348-0421.13094","DOIUrl":"10.1111/1348-0421.13094","url":null,"abstract":"<p>The skin and mucous membranes are the primary sites of <i>Staphylococcus aureus</i> colonization, particularly those of health care personnel and patients in long-term care centers. We found that <i>S. aureus</i> colonized with a higher abundance ratio on skins which had recovered from pressure injury (PI) than on normal skins in our earlier research on the skin microbiota of bedridden patients. Multilocus sequence typing (MLST) is a useful tool for typing <i>S. aureus</i> isolated from clinical specimens. However, the MLST approach cannot be used in microbiota DNA owing to the contamination from other bacteria species. In this study, we developed a multiplex-nested PCR method to determine <i>S. aureus</i> MLST in samples collected from human skins. The seven pairs of forward and reverse primers were designed in the upstream and downstream regions, which were conserved specifically in <i>S. aureus</i>. The first amplifications of the seven pairs were conducted in a multiplex assay. The samples were diluted and applied to conventional PCR for MLST. We confirmed that the method amplified the seven allele sequences of <i>S. aureus</i> specifically in the presence of untargeted DNAs from human and other skin commensal bacteria. Using this assay, we succeeded in typing sequence types (STs) of <i>S. aureus</i> in the DNA samples derived from the skins healed from PI. Peaks obtained by Sanger sequencing showed that each sample contained one ST, which were mainly categorized into clonal complex 1 (CC1) or CC5. We propose that this culture-free approach may be used in detecting <i>S. aureus</i> in clinical specimens without isolation.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"67 10","pages":"438-446"},"PeriodicalIF":2.6,"publicationDate":"2023-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10343187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}