Microbiology and Immunology最新文献_第10页

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-02-06 DOI: 10.1111/1348-0421.13118

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Lipoprotein signal peptidase-deficient Streptococcus pneumoniae exhibits impaired Toll-like receptor 2-stimulatory activity 脂蛋白信号肽酶缺陷的肺炎链球菌表现出受损的 Toll 样受体 2 刺激活性。

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-02-04 DOI: 10.1111/1348-0421.13117

Hisanori Domon, Satoru Hirayama, Toshihito Isono, Rui Saito, Katsunori Yanagihara, Yutaka Terao

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Genetic engineering employing MPB70 and its promoter enables efficient secretion and expression of foreign antigen in bacillus Calmette Guérin (BCG) Tokyo 利用 MPB70 及其启动子的基因工程技术，可以在东京卡介苗（BCG）中高效分泌和表达外来抗原。

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-01-31 DOI: 10.1111/1348-0421.13116

Atsuki Takeishi, Amina K. Shaban, Taichi Kakihana, Hayato Takihara, Shujiro Okuda, Hidekazu Osada, Desak Nyoman Surya Suameitria Dewi, Yuriko Ozeki, Yutaka Yoshida, Akihito Nishiyama, Yoshitaka Tateishi, Yuki Aizu, Yasushi Chuma, Kazuyo Onishi, Daisuke Hayashi, Saburo Yamamoto, Tetsu Mukai, Manabu Ato, Duong Huu Thai, Huynh Thi Thao Nhi, Tsuyoshi Shirai, Satoshi Shibata, Fumiko Obata, Jun Fujii, Seiya Yamayoshi, Maki Kiso, Sohkichi Matsumoto

{"title":"Genetic engineering employing MPB70 and its promoter enables efficient secretion and expression of foreign antigen in bacillus Calmette Guérin (BCG) Tokyo","authors":"Atsuki Takeishi, Amina K. Shaban, Taichi Kakihana, Hayato Takihara, Shujiro Okuda, Hidekazu Osada, Desak Nyoman Surya Suameitria Dewi, Yuriko Ozeki, Yutaka Yoshida, Akihito Nishiyama, Yoshitaka Tateishi, Yuki Aizu, Yasushi Chuma, Kazuyo Onishi, Daisuke Hayashi, Saburo Yamamoto, Tetsu Mukai, Manabu Ato, Duong Huu Thai, Huynh Thi Thao Nhi, Tsuyoshi Shirai, Satoshi Shibata, Fumiko Obata, Jun Fujii, Seiya Yamayoshi, Maki Kiso, Sohkichi Matsumoto","doi":"10.1111/1348-0421.13116","DOIUrl":"10.1111/1348-0421.13116","url":null,"abstract":"Vaccination is an important factor in public health. The recombinant bacillus Calmette Guérin (rBCG) vaccine, which expresses foreign antigens, is expected to be a superior vaccine against infectious diseases. Here, we report a new recombination platform in which the BCG Tokyo strain is transformed with nucleotide sequences encoding foreign protein fused with the MPB70 immunogenic protein precursor. By RNA-sequencing, mpb70 was found to be the most transcribed among all known genes of BCG Tokyo. Small oligopeptide, namely, polyhistidine tag, was able to be expressed in and secreted from rBCG through a process in which polyhistidine tag fused with intact MPB70 were transcribed by an mpb70 promoter. This methodology was applied to develop an rBCG expressing the receptor binding domain (RBD) of severe acute respiratory syndrome coronavirus 2. Immunoblotting images and mass spectrometry data showed that RBD was also secreted from rBCG. Sera from mice vaccinated with the rBCG showed a tendency of weak neutralizing capacity. The secretion was retained even after a freeze-drying process. The freeze-dried rBCG was administered to and recovered from mice. Recovered rBCG kept secreting RBD. Collectively, our recombination platform offers stable secretion of foreign antigens and can be applied to the development of practical rBCGs.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"68 4","pages":"130-147"},"PeriodicalIF":2.6,"publicationDate":"2024-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139642500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Isolation and whole-genome sequencing analysis of Escherichia fergusonii harboring a heat-labile enterotoxin gene from retail chicken meat in Okinawa, Japan 从日本冲绳零售鸡肉中分离出携带热嗜性肠毒素基因的弗氏大肠杆菌并对其进行全基因组测序分析。

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-01-20 DOI: 10.1111/1348-0421.13115

Tetsuya Kakita, Kenichi Lee, Masatomo Morita, Miki Okuno, Hisako Kyan, Sho Okano, Noriyuki Maeshiro, Momoko Ishizu, Tsuyoshi Kudeken, Haruno Taira, Morimi Teruya, Yoshitoshi Ogura, Yukihiro Akeda, Makoto Ohnishi

引用次数: 0

Novel role of host protein SLC25A42 in the HIV-1 reactivation of latent HIV-1 provirus 宿主蛋白 SLC25A42 在潜伏 HIV-1 病毒重新激活过程中的新作用。

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-01-20 DOI: 10.1111/1348-0421.13114

Kei Taga, Hiroaki Takeuchi

{"title":"Novel role of host protein SLC25A42 in the HIV-1 reactivation of latent HIV-1 provirus","authors":"Kei Taga, Hiroaki Takeuchi","doi":"10.1111/1348-0421.13114","DOIUrl":"10.1111/1348-0421.13114","url":null,"abstract":"Despite the effectiveness of combination antiretroviral therapy, human immunodeficiency virus (HIV) infection remains incurable. To seek new strategies to overcome HIV type 1 (HIV-1) latency, one of the major barriers to HIV elimination, it is crucial to better understand how this state is maintained. Here, by means of an RNA interference screen employing an HIV-1 latency model using monocytic cell lines, we identified solute carrier family 25 member 42 (SLC25A42) as a potential host factor not previously known to affect HIV-1 latency. SLC25A42 knockdown resulted in increased HIV-1 expression, whereas forced expression of exogenous SLC25A42 suppressed it in SLC25A42-depleted cells. SLC25A42 depletion increased HIV-1 proviral transcriptional elongation but did not cause HIV-1 activation in an HIV-1 Tat-depleted latency model. This suggests that the role of SLC25A42 in HIV-1 transcription depends on HIV-1 Tat. Chromatin immunoprecipitation-qPCR analysis further revealed that SLC25A42 accumulated on or near the HIV-1 5ʹ long terminal repeat promoter region of the HIV-1 provirus, suggesting a possible role in regulating HIV-1 Tat near this promoter region. These results indicate that SLC25A42 plays a novel role in HIV-1 latency maintenance in monocytic HIV-1 reservoirs.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"68 3","pages":"90-99"},"PeriodicalIF":2.6,"publicationDate":"2024-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.13114","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139512931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral and rectal colonization of methicillin-resistant Staphylococcus aureus in long-term care facility residents and their association with clinical status 长期护理机构居民耐甲氧西林金黄色葡萄球菌的口腔和直肠定植及其与临床状况的关系。

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-01-17 DOI: 10.1111/1348-0421.13111

Satoru Kusaka, Azusa Haruta, Miki Kawada-Matsuo, Mi Nguyen-Tra Le, Mineka Yoshikawa, Toshiki Kajihara, Koji Yahara, Junzo Hisatsune, Ryota Nomura, Kazuhiro Tsuga, Hiroki Ohge, Motoyuki Sugai, Hitoshi Komatsuzawa

{"title":"Oral and rectal colonization of methicillin-resistant Staphylococcus aureus in long-term care facility residents and their association with clinical status","authors":"Satoru Kusaka, Azusa Haruta, Miki Kawada-Matsuo, Mi Nguyen-Tra Le, Mineka Yoshikawa, Toshiki Kajihara, Koji Yahara, Junzo Hisatsune, Ryota Nomura, Kazuhiro Tsuga, Hiroki Ohge, Motoyuki Sugai, Hitoshi Komatsuzawa","doi":"10.1111/1348-0421.13111","DOIUrl":"10.1111/1348-0421.13111","url":null,"abstract":"Staphylococcus aureus is a commensal bacterium in humans, but it sometimes causes opportunistic infectious diseases such as suppurative skin disease, pneumonia, and enteritis. Therefore, it is important to determine the prevalence of S. aureus and methicillin-resistant S. aureus (MRSA) in individuals, especially older adults. In this study, we investigated the prevalence of S. aureus and MRSA in the oral cavity and feces of residents in long-term care facilities (LTCFs). S. aureus was isolated from the oral cavity of 61/178 (34.3%) participants, including 28 MRSA-positive participants (15.7%), and from the feces of 35/127 (27.6%) participants, including 16 MRSA-positive participants (12.6%). S. aureus and MRSA were isolated from both sites in 19/127 individuals (15.0%) and 10/127 individuals (7.9%), respectively. Among 19 participants with S. aureus isolation from both sites, 17 participants showed the same sequence type (ST) type. Then, we analyzed the correlation of S. aureus and MRSA in the oral cavity and rectum with the participant's condition. S. aureus and MRSA positivity in the oral cavity was significantly related to tube feeding, while there was no correlation of rectal S. aureus/MRSA with any factors. Our findings regarding the oral inhabitation of MRSA and its risk factors indicate the importance of considering countermeasures against MRSA infection in LTCFs.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"68 3","pages":"75-89"},"PeriodicalIF":2.6,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139478884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strain identity of Bordetella pertussis isolates from household members based on whole-genome sequencing 根据全基因组测序鉴定从家庭成员中分离出的百日咳博德特氏菌的菌株特征。

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-01-15 DOI: 10.1111/1348-0421.13113

Kazunari Kamachi, Kentaro Koide, Nao Otsuka, Masataka Goto, Tsuyoshi Kenri

引用次数: 0

Issue Information – Cover 发行信息 - 封面

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2024-01-04 DOI: 10.1111/1348-0421.13112

引用次数: 0

Circ_0008410 contributes to fibroblast-like synoviocytes dysfunction by regulating miR-149-5p/HIPK2 axis Circ_0008410 通过调控 miR-149-5p/HIPK2 轴导致成纤维细胞样滑膜细胞功能障碍。

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-12-21 DOI: 10.1111/1348-0421.13110

Wensi Su, Zhifang Ye, Guangji Wang, Hui Huang, Yehan Fang

{"title":"Circ_0008410 contributes to fibroblast-like synoviocytes dysfunction by regulating miR-149-5p/HIPK2 axis","authors":"Wensi Su, Zhifang Ye, Guangji Wang, Hui Huang, Yehan Fang","doi":"10.1111/1348-0421.13110","DOIUrl":"10.1111/1348-0421.13110","url":null,"abstract":"Circular RNAs (circRNAs) play functional roles in rheumatoid arthritis (RA) progression. Fibroblast-like synoviocytes (RASFs) are the main effectors in RA development. In this study, we explored the function and mechanism of circ_0008410 in RASFs. qRT-PCR was used to detect the expression of circ_0008410, microRNA-149-5p (miR-149-5p), and homeodomain-interacting protein kinase 2 (HIPK2). Cell counting kit-8, EdU assay, flow cytometry, and transwell assay were performed to evaluate cell proliferation, apoptosis, migration, and invasion. Western blot measured the protein levels of related markers and HIPK2. The levels of IL-1β, TNF-α, and IL-6 were tested by corresponding ELISA kits and Western blot. The combination between miR-149-5p and circ_0008410 or HIPK2 was detected by dual-luciferase reporter assay or RNA immunoprecipitation (RIP) assay. Our data showed that circ_0008410 and HIPK2 were elevated, while miR-149-5p was downregulated in RA synovial tissues and RASFs. Circ_0008410 promoted RASF proliferation, migration, invasion, and inflammation while inhibiting apoptosis. MiR-149-5p was a target of circ_0008410, and its overexpression could reverse the promoting effects of circ_0008410 on RASF dysfunction. Moreover, miR-149-5p could target HIPK2 to suppress RASF proliferation, migration, invasion, and inflammation. Collectively, circ_0008410 promoted RASF dysfunction via miR-149-5p/HIPK2, which might provide a potential target for RA therapy.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"68 3","pages":"100-110"},"PeriodicalIF":2.6,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138830485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RpoN (sigma factor 54) contributes to bacterial fitness during tracheal colonization of Bordetella bronchiseptica RpoN（sigma factor 54）有助于支气管败血波氏杆菌气管定植过程中的细菌适应性

IF 2.6 4区医学

Microbiology and Immunology Pub Date : 2023-12-17 DOI: 10.1111/1348-0421.13109

Xingyan Ma, Dendi K. Nugraha, Yukihiro Hiramatsu, Yasuhiko Horiguchi

{"title":"RpoN (sigma factor 54) contributes to bacterial fitness during tracheal colonization of Bordetella bronchiseptica","authors":"Xingyan Ma, Dendi K. Nugraha, Yukihiro Hiramatsu, Yasuhiko Horiguchi","doi":"10.1111/1348-0421.13109","DOIUrl":"10.1111/1348-0421.13109","url":null,"abstract":"The Gram-negative pathogenic bacterium Bordetella bronchiseptica is a respiratory pathogen closely related to Bordetella pertussis, the causative agent of whooping cough. Despite sharing homologous virulence factors, B. bronchiseptica infects a broad range of mammalian hosts, including some experimental animals, whereas B. pertussis is strictly adapted to humans. Therefore, B. bronchiseptica is often used as a representative model to explore the pathogenicity of Bordetella in infection experiments with laboratory animals. Although Bordetella virulence factors, including toxins and adhesins have been studied well, our recent study implied that unknown virulence factors are involved in tracheal colonization and infection. Here, we investigated bacterial genes contributing to tracheal colonization by high-throughput transposon sequencing (Tn-seq). After the screening, we picked up 151 candidate genes of various functions and found that a rpoN-deficient mutant strain was defective in tracheal colonization when co-inoculated with the wild-type strain. rpoN encodes σ54, a sigma factor that regulates the transcription of various genes, implying its contribution to various bacterial activities. In fact, we found RpoN of B. bronchiseptica is involved in bacterial motility and initial biofilm formation. From these results, we propose that RpoN supports bacterial colonization by regulating various bacteriological functions.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"68 2","pages":"36-46"},"PeriodicalIF":2.6,"publicationDate":"2023-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.13109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138714334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0