A fungal-binding agglutinin in the skin slime of Japanese flounder (Paralichthys olivaceus) is glyceraldehyde 3-phosphate dehydrogenase

IF 1.9 4区 医学 Q4 IMMUNOLOGY
Shigeyuki Tsutsui, Mizuki Terashima, Osamu Nakamura
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引用次数: 0

Abstract

Agglutination of pathogenic microorganisms on the body surface is a significant phenomenon for the prevention of infection. In the present study, we show that an extract of the skin mucus from Japanese flounder (Paralichthys olivaceus) has agglutination activity against the yeast Saccharomyces cerevisiae. We purified this yeast-binding protein, which consists of an approximately 35-kDa homodimer, using affinity chromatography with yeast as a ligand. Multiple internal amino acid sequences of the protein, as determined using liquid chromatography with quadrupole time-of-flight tandem mass spectrometry, mapped to flounder glyceraldehyde 3-phosphate dehydrogenase (GAPDH). An anti-GAPDH antibody inhibited the yeast agglutination activity in the skin mucus extract and stained agglutinated yeast, indicating that flounder GAPDH could agglutinate yeast. The current study suggests that GAPDH, a well-known protein as the sixth enzyme in the glycolytic pathway, is a significant player in mucosal immunity in teleosts.

日本鲽(Paralichthys olivaceus)皮肤粘液中的一种真菌结合凝集素是 3-磷酸甘油醛脱氢酶。
在体表凝集病原微生物是预防感染的一个重要现象。在本研究中,我们发现日本比目鱼(Paralichthys olivaceus)皮肤粘液提取物具有凝集酵母菌的活性。我们以酵母为配体,利用亲和层析技术纯化了这种酵母结合蛋白,它由一个约 35 kDa 的同源二聚体组成。通过液相色谱-四极杆飞行时间串联质谱测定,该蛋白的多个内部氨基酸序列与比目鱼甘油醛-3-磷酸脱氢酶(GAPDH)相对应。抗 GAPDH 抗体可抑制皮肤粘液提取物中的酵母凝集活性,并对凝集的酵母进行染色,表明比目鱼 GAPDH 可凝集酵母。本研究表明,作为糖酵解途径中第六种酶的著名蛋白质 GAPDH 在远洋鱼类的粘膜免疫中起着重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microbiology and Immunology
Microbiology and Immunology 医学-免疫学
CiteScore
5.20
自引率
3.80%
发文量
78
审稿时长
1 months
期刊介绍: Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.
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