Microbiology and Immunology最新文献_第2页

Cytotoxic T Lymphocyte Density and PD-L1 Expression Predict the Response to Anti-PD1 Therapy in Recurrent Oral Squamous Cell Carcinoma 细胞毒性T淋巴细胞密度和PD-L1表达预测复发性口腔鳞状细胞癌抗pd1治疗的反应。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2025-04-13 DOI: 10.1111/1348-0421.13220

Mayuko Yamashita, Hiromu Yano, Yoshihiro Komohara, Rin Yamada, Yukio Fujiwara, Masatoshi Hirayama, Yuki Seki, Ryoji Yoshida, Hideki Nakayama

{"title":"Cytotoxic T Lymphocyte Density and PD-L1 Expression Predict the Response to Anti-PD1 Therapy in Recurrent Oral Squamous Cell Carcinoma","authors":"Mayuko Yamashita, Hiromu Yano, Yoshihiro Komohara, Rin Yamada, Yukio Fujiwara, Masatoshi Hirayama, Yuki Seki, Ryoji Yoshida, Hideki Nakayama","doi":"10.1111/1348-0421.13220","DOIUrl":"10.1111/1348-0421.13220","url":null,"abstract":"<div>\u0000 \u0000 <p>Oral squamous cell carcinoma (OSCC) is one of the most common head and neck cancers, and immunotherapy targeting programmed cell death 1 (PD-1) has become a treatment option for recurrent OSCC after surgery and radiation therapy. However, few studies have identified definitive biomarkers for predicting patient response to anti-PD1 therapy in OSCC. In the present study, biopsy specimens were obtained from 23 patients with recurrent OSCC who were subsequently treated with anti-PD1 therapy. Immunohistochemical examinations of CD3, CD8, FOXP3, CD103, CD163, programmed cell death ligand 1 (PD-L1), HLA-A/B/C, HLA-DR, and β2 microglobulin were performed, and their correlation with clinical response was statistically analyzed. We found that an increased density of CD8-positive lymphocytes and increased PD-L1 expression predicted a favorable response to anti-PD1 therapy in recurrent OSCC. In contrast, clinical factors such as age and sex, and immune-related factors such as HLA-Classes I and II, were not associated with the response to anti-PD1 therapy. Taken together, our results suggest that immunohistochemical analysis of CD8 and PD-L1 may be useful for predicting the efficacy of anti-PD1 therapy in recurrent OSCC.</p>\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"69 6","pages":"350-358"},"PeriodicalIF":1.9,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crohn's Disease‒Associated Escherichia coli BasRS Is Involved in Fimbriae Expression, Contributing to Epithelial Cell Invasion 克罗恩病相关大肠杆菌BasRS参与菌毛表达，促进上皮细胞侵袭

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2025-04-13 DOI: 10.1111/1348-0421.13221

Tsuyoshi Miki, Masahiro Ito, Takeshi Haneda, Yun-Gi Kim

引用次数: 0

Expression Purification and Immunogenicity Detection of HtsA + FtsB Fusion Protein From Streptococcus pyogenes 化脓性链球菌HtsA + FtsB融合蛋白的表达、纯化及免疫原性检测

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2025-04-11 DOI: 10.1111/1348-0421.13217

Jing Li, Yan Ju, Min Jiang, Jing-Xi Wang, Sha Li, Xiao-Yan Yang

{"title":"Expression Purification and Immunogenicity Detection of HtsA + FtsB Fusion Protein From Streptococcus pyogenes","authors":"Jing Li, Yan Ju, Min Jiang, Jing-Xi Wang, Sha Li, Xiao-Yan Yang","doi":"10.1111/1348-0421.13217","DOIUrl":"10.1111/1348-0421.13217","url":null,"abstract":"<div>\u0000 \u0000 <p>Lipoproteins are a class of potential vaccine candidates for <i>Streptococcus pyogenes</i>. The present study was conducted to purify and detect the immunogenicity of the fusion protein HtsA + FtsB of the iron transport lipoproteins HtsA and FtsB of <i>S. pyogenes</i>. The recombinant expression vector pBAD-<i>htsA</i> + <i>ftsB</i> was successfully constructed, and the fusion protein HtsA + FtsB with a purity above 95% was successfully obtained. Western blot analysis confirmed that the HtsA + FtsB fusion protein had good antigenicity and could be specifically recognized by both HtsA antiserum and FtsB antisera, and the antibody specificity of the HtsA + FtsB fusion protein was good. ELISA results showed that IgG antibody levels were significantly increased in the HtsA + FtsB fusion protein immunization group compared to the PBS control group. Additionally, cytokine levels, including IL-2, IFN-γ, IL-4, IL-6, and IL-17A, were significantly elevated. Furthermore, the antiserum from HtsA + FtsB-immunized mice enhanced the opsonophagocytic activity against <i>S. pyogenes</i>. The HtsA + FtsB fusion protein has strong immunogenicity and potential as a candidate vaccine for <i>S. pyogenes</i>.</p>\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"69 6","pages":"317-325"},"PeriodicalIF":1.9,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTION: Chlamydia Pneumoniae Induces Interleukin-6 and Interleukin-10 in Human Gingival Fibroblastsle 撤回：肺炎衣原体诱导人牙龈成纤维细胞中白细胞介素-6和白细胞介素-10。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2025-04-08 DOI: 10.1111/1348-0421.13218

引用次数: 0

Issue Information – Cover 发行资料-封面

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2025-04-07 DOI: 10.1111/1348-0421.13137

引用次数: 0

FTO Aggravates the Infiltration of Inflammatory Cells and Pulmonary Fibrosis in Silicosis Though Inducing the Imbalance of Macrophages Polarization FTO通过诱导巨噬细胞极化失衡，加重矽肺炎症细胞浸润和肺纤维化。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2025-04-01 DOI: 10.1111/1348-0421.13216

Jian-min Fan, Xu Zhang

{"title":"FTO Aggravates the Infiltration of Inflammatory Cells and Pulmonary Fibrosis in Silicosis Though Inducing the Imbalance of Macrophages Polarization","authors":"Jian-min Fan, Xu Zhang","doi":"10.1111/1348-0421.13216","DOIUrl":"10.1111/1348-0421.13216","url":null,"abstract":"<div>\u0000 \u0000 <p>Silicosis is a lung disease that is very harmful. This makes the disease worse. This study looks at how the fat mass and obesity associated (FTO) gene affects macrophage M1/M2 polarisation and pulmonary fibrosis in silicosis. Macrophages were isolated from alveolar lavage fluid in silicosis and bronchiectasis (BE) patients. Gene expression was detected by reverse transcription-PCR (RT-PCR). Pulmonary fibrosis was assessed by CT<sub>FLV/TLV%</sub> using 3D CT and Masson staining assay. Enzyme-linked immunosorbent assay was applied to assess inflammatory factor level. The macrophage M1/M2 polarization characteristics (iNOS, CD206) was quantified by Immunofluorescence and Flow cytometry assays. Silicosis patients alveolar lavage macrophages polarized towards M1 type, and the expression level of M1 polarization-related chemokines also increased. More importantly, FTO gene downregulation promotes macrophage polarization to M1 type and the secretion of pro-inflammatory factor TNF-α and IL-6. And FTO knockdown can strengthen the glycolysis of macrophages, especially anaerobic glycolysis, thus inducing macrophages M1 polarization. Moreover, downregulation of FTO ameliorates silicosis pulmonary fibrosis. And FTO upregulation is associated with the M2 polarization of macrophage and the deterioration of pulmonary fibrosis in silicosis patients. FTO downregulation facilitates the infiltration of inflammatory cells by promoting M1 polarization of macrophages in silicosis.</p>\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"69 6","pages":"339-349"},"PeriodicalIF":1.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTION: Expression of Small RNAs of Bordetella Pertussis Colonizing Murine Tracheas 缩回：百日咳杆菌定植小鼠气管小rna的表达。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2025-03-31 DOI: 10.1111/1348-0421.13214

{"title":"RETRACTION: Expression of Small RNAs of Bordetella Pertussis Colonizing Murine Tracheas","authors":"","doi":"10.1111/1348-0421.13214","DOIUrl":"10.1111/1348-0421.13214","url":null,"abstract":"<p><b>RETRACTION</b>: Y. Hiramatsu, K. Suzuki, D. Motooka, S. Nakamura, Y. Horiguchi, “Expression of Small RNAs of Bordetella Pertussis Colonizing Murine Tracheas,” <i>Microbiology and Immunology</i> 64, no. 6 (2020): 469–475, https://doi.org/10.1111/1348-0421.12791.</p><p>The above article, published online on 30 March, 2020 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors; the journal Editors-in-Chief, Chikara Kaito, Tomoyuki Honda, and Tomohiro Sawa; the Japanese Society for Bacteriology, the Japanese Society for Virology, and the Japanese Society for Host Defense Research; and John Wiley & Sons Australia, Ltd. The retraction has been agreed due to the request from the last author and the mutual agreement among all authors, after an institutional investigation was conducted by Osaka University that recommended retraction. The institutional investigation revealed that the first author was responsible for data fabrication and falsification within Figure 1(b), Figure 1(c) and data fabrication in Figure 2 for the Bpr4, 8 panels. Therefore, the conclusions of the paper are substantially compromised.</p>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"69 5","pages":"315"},"PeriodicalIF":1.9,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.13214","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RETRACTION: Current Understanding of Bordetella-Induced Cough 撤回：目前对博德泰拉引起的咳嗽的理解。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2025-03-25 DOI: 10.1111/1348-0421.13215

引用次数: 0

Correction to “Bordet-Gengou Agar Medium Supplemented With Albumin-Containing Biologics for Cultivation of Bordetellae” 对“博德-根沟琼脂培养基中添加含白蛋白生物制剂培养博德菌”的修正。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2025-03-24 DOI: 10.1111/1348-0421.13212

引用次数: 0

Correction to “Identification of the Minimum Region of Bordetella pertussis Vag8 Required for Interaction With C1 Inhibitor” 更正“百日咳杆菌Vag8与C1抑制剂相互作用所需的最小区域的鉴定”。

IF 1.9 4区医学

Microbiology and Immunology Pub Date : 2025-03-24 DOI: 10.1111/1348-0421.13213

引用次数: 0