Microbiology and Immunology最新文献_第2页

The Bordetella Effector BteA Interacts With BopN Through Its C-Terminal Region, Thereby Controlling Its Translocation Into Host Cells. 博德特拉效应体BteA通过BopN的c端区与BopN相互作用，从而控制其转运到宿主细胞。

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-09-29 DOI: 10.1111/1348-0421.70093

Toshinobu Ogawa, Asaomi Kuwae, Takashi Matsui, Maki Tokura, Akira Haku, Akio Abe

{"title":"The Bordetella Effector BteA Interacts With BopN Through Its C-Terminal Region, Thereby Controlling Its Translocation Into Host Cells.","authors":"Toshinobu Ogawa, Asaomi Kuwae, Takashi Matsui, Maki Tokura, Akira Haku, Akio Abe","doi":"10.1111/1348-0421.70093","DOIUrl":"https://doi.org/10.1111/1348-0421.70093","url":null,"abstract":"BteA is a cytotoxic effector translocated into host cells via the type III secretion system (T3SS) of Bordetella. The T3SS gatekeeper protein BopN controls BteA translocation, yet the BteA region mediating this regulation remains unknown. Here, we demonstrate that BteA interacts with BopN through its C-terminal region, and this interaction is required for efficient translocation into host cells. Using a CyaA-based translocation assay, we showed that BteA comprising the N-terminal 448 amino acids (BteA-N448) and BteA with a deletion of amino acids 249-448 (BteA-∆249-448) were translocated in a BopN-dependent manner, whereas BteA comprising the N-terminal 248 amino acids (BteA-N248) was translocated independently of BopN. Pull-down assays confirmed that BteA-N448 and BteA-Δ249-448 physically interact with BopN, whereas BteA-N248 does not. ColabFold-based structure prediction identified candidate interface residues, and subsequent mutagenesis revealed that simultaneous alanine substitution of aspartate 263 (D263) and phenylalanine 340 (F340) abolished BopN-dependent translocation. Our results demonstrated that the amino acid residues D263 and F340 in BteA play important roles in BopN-mediated translocation control. Furthermore, the C-terminal 14-amino-acid region of BteA was strongly suggested to be involved in this regulation. The present study provides new insights into the effector-gatekeeper network that controls T3SS-mediated pathogenicity in Bordetella.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149075260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic Effect of Ambient Ozone, Relative Humidity, and Temperature on Epidemic Patterns of Influenza: A Hypothesis and Its Verification. 环境臭氧、相对湿度和温度对流感流行模式的协同效应：一个假设及其验证。

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-09-16 DOI: 10.1111/1348-0421.70089

Hidekazu Nishimura, Soichiro Sakata, Julian W Tang, Suguru Ohmiya, Masanori Katsumi, Takahiro Yokoyama

{"title":"Synergistic Effect of Ambient Ozone, Relative Humidity, and Temperature on Epidemic Patterns of Influenza: A Hypothesis and Its Verification.","authors":"Hidekazu Nishimura, Soichiro Sakata, Julian W Tang, Suguru Ohmiya, Masanori Katsumi, Takahiro Yokoyama","doi":"10.1111/1348-0421.70089","DOIUrl":"https://doi.org/10.1111/1348-0421.70089","url":null,"abstract":"The mechanisms underlying seasonal patterns in influenza epidemics have long been an enigma. Our laboratory study revealed that ambient ozone significantly inactivates airborne influenza viruses synergistically with higher relative humidity and temperature. This led us to hypothesize that natural seasonal fluctuations in these ambient factors enhance or mitigate the influenza-transmission risk by altering the efficiency of airborne virus inactivation, thereby contributing to the continuation or subsiding of influenza epidemics. To test the hypothesis, we examined datasets covering 5 years of annual environmental ozone concentrations, relative humidity, and temperature, and reported influenza case numbers in two cities in Japan, Sendai and Naha, which exhibit typical temperate and subtropical climate patterns, respectively; based on the findings of our previous study and actual ambient environmental data, we estimated the influenza airborne-transmission risk in these cities and compared it with observed epidemics. In both cities, the persistence of winter epidemics was strongly associated with low ozone concentration (< 35 ppb), and their subsiding in spring was associated with increases in ozone (≧ 35 ppb) and relative humidity (≧ 50%), even at lower temperature (< 20°C). The summer outbreak in Naha was not suppressed solely under high temperatures (25°C-30°C) and relative humidity (≧ 70%), but was associated with a subsequent increase in ozone (≧ 35 ppb). A similar phenomenon was recognized during the 2009 influenza pandemic. These results were consistent with our hypothesis: influenza epidemic patterns corresponded with the influenza transmission risk estimated from the synergistic inactivation patterns of airborne viruses by ambient ozone, relative humidity, and temperature.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148977149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exopolysaccharides From Lactobacillus delbrueckii subsp. bulgaricus OLL1073R-1 Enhance Macrophage Bactericidal Activity Through IL-12-Dependent Macrophage-NK Cell Crosstalk. 德尔布鲁氏乳杆菌亚种的胞外多糖。OLL1073R-1通过il -12依赖性巨噬细胞- nk细胞串扰增强巨噬细胞杀菌活性。

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-09-14 DOI: 10.1111/1348-0421.70090

Chikara Kohda, Hiroki Ishikawa, Yoshihiro Kuno, Yasunari Matsuzaka, Hiroki Kono, Toshihiro Sashihara, Hirotaka Kuwata, Masayuki Iyoda

{"title":"Exopolysaccharides From Lactobacillus delbrueckii subsp. bulgaricus OLL1073R-1 Enhance Macrophage Bactericidal Activity Through IL-12-Dependent Macrophage-NK Cell Crosstalk.","authors":"Chikara Kohda, Hiroki Ishikawa, Yoshihiro Kuno, Yasunari Matsuzaka, Hiroki Kono, Toshihiro Sashihara, Hirotaka Kuwata, Masayuki Iyoda","doi":"10.1111/1348-0421.70090","DOIUrl":"https://doi.org/10.1111/1348-0421.70090","url":null,"abstract":"Exopolysaccharides (EPS) produced by Lactobacillus delbrueckii subsp. bulgaricus OLL1073R-1 have been reported to exert immunomodulatory effects, primarily in antiviral responses; however, their role in antibacterial host defense remains unclear. Here, we investigated whether EPS enhance macrophage-mediated killing of Streptococcus pyogenes, a facultative intracellular pathogen, using an in vitro co-culture system with NK-like cells. EPS alone did not enhance the intracellular bactericidal activity of macrophages. In contrast, EPS significantly increased bacterial killing when macrophages were co-cultured with NK-like cells. Mechanistically, EPS induced interleukin (IL)-12 expression in macrophages, while interferon (IFN)-γ production was not detected when macrophages or NK-like cells were cultured separately, but was significantly induced in the co-culture system. Neutralization of IL-12 or IFN-γ significantly abrogated the EPS-mediated enhancement of bactericidal activity, indicating that both cytokines are required for this effect. These findings support a model in which EPS stimulate macrophages to produce IL-12, leading to NK cell activation and subsequent IFN-γ-dependent enhancement of macrophage bactericidal function. Our results reveal a previously uncharacterized role of probiotic-derived EPS in promoting antibacterial immunity through macrophage-NK cell crosstalk.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148970137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variation in the Milk Microbiota of Healthy Dairy Cows Across Parity and Lactation Stage. 健康奶牛胎次和泌乳期乳微生物群的变化。

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-09-14 DOI: 10.1111/1348-0421.70091

Nobuki Yoshizawa, Ayuka Mitsuya, Tsuyoshi Sekizuka, Yoshio Kiku, Ikuo Uchida, Masato Akiba

{"title":"Variation in the Milk Microbiota of Healthy Dairy Cows Across Parity and Lactation Stage.","authors":"Nobuki Yoshizawa, Ayuka Mitsuya, Tsuyoshi Sekizuka, Yoshio Kiku, Ikuo Uchida, Masato Akiba","doi":"10.1111/1348-0421.70091","DOIUrl":"https://doi.org/10.1111/1348-0421.70091","url":null,"abstract":"To characterize variation in the milk microbiota of clinically healthy dairy cows, milk samples were collected repeatedly from four cows throughout lactation and analyzed by 16S rRNA gene amplicon sequencing. Alpha-diversity analysis showed no significant effects of parity, lactation stage, udder quarter position, or the interaction between lactation stage and position. Although sample-level PERMANOVA indicated significant associations of beta diversity with cow identity and parity, these associations were not supported when the repeated-measures structure was appropriately accounted for. Restricted-permutation PERMANOVA showed no significant effects of lactation stage, udder quarter position, or their interaction, and exact permutation testing at the individual-cow level showed no significant effect of parity. Ordination and hierarchical clustering analyzes nevertheless revealed substantial variation in bacterial community composition among individual samples. Hierarchical clustering based on genus-level relative abundance separated most samples into two major groups characterized by relatively high and low diversity. The high-diversity group showed a relatively high abundance of the class Clostridia, whereas the low-diversity group was characterized by a predominance of Gammaproteobacteria, particularly Escherichia/Shigella in some samples. Overall, the milk microbiota of healthy dairy cows showed substantial sample-to-sample variation, but the present data did not provide clear evidence that parity, lactation stage, or udder quarter position systematically influenced microbial diversity. These findings provide baseline information on variation in the milk microbiota of healthy dairy cows and highlight the importance of accounting for repeated measurements and individual variation in studies of the bovine milk microbiota.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148970167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aerobic Exercise Mitigates Experimental Knee Osteoarthritis via Inhibition of cGAS–STING Signaling 有氧运动通过抑制cGAS-STING信号通路减轻实验性膝关节骨关节炎。

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-09-08 Epub Date: 2026-07-01 DOI: 10.1111/1348-0421.70074

Yini Mao, Baojun Li, Jinjun Dai, Yichuan Peng, Ying Huang, Ke Wu

{"title":"Aerobic Exercise Mitigates Experimental Knee Osteoarthritis via Inhibition of cGAS–STING Signaling","authors":"Yini Mao, Baojun Li, Jinjun Dai, Yichuan Peng, Ying Huang, Ke Wu","doi":"10.1111/1348-0421.70074","DOIUrl":"10.1111/1348-0421.70074","url":null,"abstract":"<div>\u0000 \u0000 Knee osteoarthritis (KOA) is a chronic joint disorder characterized by cartilage deterioration. lacking disease-modifying therapies. Aerobic exercise is recommended as a first-line non-pharmacological intervention for KOA, but its molecular mechanisms remain unclear. Recent studies indicate that the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway, a key regulator of inflammation and cartilage homeostasis, may mediate the protective effects of aerobic exercise on joint integrity. Male C57BL/6 mice underwent destabilization of the medial meniscus (DMM) surgery and were assigned to Sham, Aerobic exercise, DMM, and DMM + Aerobic exercise groups. Aerobic treadmill running was performed for 4 weeks, with a STING agonist (DMXAA) and STING inhibitor (C-176) used to verify pathway involvement. Joint function, cartilage histology, inflammatory cytokines, ECM metabolism, chondrocyte apoptosis, and cGAS-STING pathway activation, including phosphorylation levels and 2′3′-cGAMP production, were assessed. DMM induced joint swelling, gait impairment, cartilage degradation, ECM catabolism, chondrocyte apoptosis, inflammation, and robust cGAS-STING pathway activation. Aerobic exercise significantly ameliorated these changes, improving joint function, preserving cartilage and ECM integrity, reducing apoptosis, suppressing inflammatory cytokines, and directly inhibiting cGAS-STING signaling. STING inhibition alone exerted comparable chondroprotective effects to aerobic exercise, and no additional benefit was observed in combination. DMXAA treatment abolished these protective effects. Aerobic exercise alleviates DMM-induced knee osteoarthritis by enhancing joint function and preserving cartilage integrity, effects that are directly mediated through suppression of the cGAS-STING pathway. These findings establish a causal link between aerobic exercise and innate immune regulation in osteoarthritis.</div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 9","pages":"416-426"},"PeriodicalIF":1.6,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148361588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A N-Acetyl-D-Glucosamine-Binding C-Type Lectin in the Cutaneous Mucus of Chub Mackerel, Scomber japonicus, Agglutinates Pathogenic Bacteria 一种n -乙酰基- d -氨基葡萄糖结合c型凝集素在鲐鱼、日本鲭鱼皮肤黏液中凝集致病菌。

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-09-08 Epub Date: 2026-07-22 DOI: 10.1111/1348-0421.70078

Shigeyuki Tsutsui, Takuya Kunisaki, Tatsuto Izawa, Osamu Nakamura

{"title":"A N-Acetyl-D-Glucosamine-Binding C-Type Lectin in the Cutaneous Mucus of Chub Mackerel, Scomber japonicus, Agglutinates Pathogenic Bacteria","authors":"Shigeyuki Tsutsui, Takuya Kunisaki, Tatsuto Izawa, Osamu Nakamura","doi":"10.1111/1348-0421.70078","DOIUrl":"10.1111/1348-0421.70078","url":null,"abstract":"<div>\u0000 \u0000 A C-type lectin with specificity for N-acetyl-d-glucosamine was purified from the cutaneous mucus of chub mackerel (Scomber japonicus) by affinity chromatography. The lectin comprises 159 amino acid residues and contains an unusual substitution within the conserved Ca2+-binding motif, in which Trp–Asn–Asp is replaced by Trp–Ser–Asp. Reverse transcription–PCR analysis demonstrated that the lectin gene is specifically expressed in the skin, and immunohistochemistry localized the protein to the outer epidermal layer, predominantly in squamous cells. Agglutinating activity was observed against L. garvieae, but not against any of the other bacterial species tested. An approximately 25-kDa bacterial protein was identified as a potential ligand, although its identity remains unresolved. Collectively, these findings indicate that this lectin (SjCL) functions as a mucosal immune molecule and may contribute to the first line of defense against bacterial pathogens in teleost fish.\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 9","pages":"436-447"},"PeriodicalIF":1.6,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148549856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and Genomic Characterization of mcr-Positive Enterobacteriaceae in Retail Meat in Thailand Following the Colistin Ban 在泰国禁止粘菌素后，零售肉类中mcr阳性肠杆菌科的流行和基因组特征。

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-09-08 Epub Date: 2026-07-06 DOI: 10.1111/1348-0421.70073

Ahmed Abououf, Sharda Prasad Awasthi, Asmaa M. Elbastawesy, Noritoshi Hatanaka, Atsushi Hinenoya, Srinuan Somroop, Yukihiro Kaneko, Shinji Yamasaki

{"title":"Prevalence and Genomic Characterization of mcr-Positive Enterobacteriaceae in Retail Meat in Thailand Following the Colistin Ban","authors":"Ahmed Abououf, Sharda Prasad Awasthi, Asmaa M. Elbastawesy, Noritoshi Hatanaka, Atsushi Hinenoya, Srinuan Somroop, Yukihiro Kaneko, Shinji Yamasaki","doi":"10.1111/1348-0421.70073","DOIUrl":"10.1111/1348-0421.70073","url":null,"abstract":"This study aimed to investigate the prevalence and characteristics of mcr-positive Enterobacteriaceae in retail meat in Thailand following the national ban on prophylactic colistin use in food producing animals. A total of 152 meat samples (103 chicken and 49 pork) were collected from supermarkets and open markets between July and September 2023. Samples were screened for mcr-1 to mcr-5 using multiplex PCR. None of the samples from supermarkets tested positive, whereas mcr genes were detected in 15.4% (6/39) and 13.3% (4/30) of chicken and pork samples, respectively, from open markets, with mcr-1 and/or mcr-3 identified. A total of 21 isolates were recovered from PCR-positive samples (11 from chicken and 10 from pork). Escherichia coli was the predominant species (n = 19), followed by Klebsiella pneumoniae (n = 2). All mcr-positive isolates exhibited multidrug resistance. Whole-genome sequencing was performed for 19 non-clonal isolates. One K. pneumoniae strain from a pork sample co-harbored mcr-1 and mcr-8, representing the first report of this combination in the animal sector in Thailand. In addition, virulence-associated genes, including adhesion factors, toxins, and iron acquisition systems, were identified in selected isolates. Core genome SNP-based phylogenetic analysis revealed substantial genomic diversity among the isolates, suggesting relatedness to strains reported prior to the colistin ban. These findings indicate that retail meat from open markets may serve as an important route for the transmission of mcr-positive bacteria in Thailand and highlight the urgent need to incorporate systematic retail meat surveillance into national antimicrobial resistance monitoring programs.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 9","pages":"402-415"},"PeriodicalIF":1.6,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.70073","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Two Competition-Defined Neutralizing Antibody Groups Targeting the HHV-6B gH/gL/gQ1/gQ2 Complex Independent of CD134 Binding 两种不依赖CD134靶向HHV-6B gH/gL/gQ1/gQ2复合物的竞争定义中和抗体群的鉴定

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-09-08 Epub Date: 2026-07-17 DOI: 10.1111/1348-0421.70076

Achmad Januar Er Putra, Ida Ayu Gde Wahyudevi Dharmika, Natsumi Hasegawa, Regina Rachel Gunawan, Rei Takamiya, Mitsuhiro Nishimura, Yasuko Mori

{"title":"Identification of Two Competition-Defined Neutralizing Antibody Groups Targeting the HHV-6B gH/gL/gQ1/gQ2 Complex Independent of CD134 Binding","authors":"Achmad Januar Er Putra, Ida Ayu Gde Wahyudevi Dharmika, Natsumi Hasegawa, Regina Rachel Gunawan, Rei Takamiya, Mitsuhiro Nishimura, Yasuko Mori","doi":"10.1111/1348-0421.70076","DOIUrl":"10.1111/1348-0421.70076","url":null,"abstract":"<div>\u0000 \u0000 Human herpesvirus 6B (HHV-6B) entry is mediated by the interaction between gH/gL/gQ1/gQ2 complex (tetramer) and CD134 expressed on activated T cells. In this study, we identified and characterized a panel of monoclonal antibodies targeting the HHV-6B tetramer, with particular focus on three antibodies; gHgL J1, gHgL O2, and gHgL F5; that represent anti-gH neutralizing monoclonal antibodies with binding characteristics distinct from the previously described antibody OHV-3. Immunofluorescence and Western blot analyses confirmed recognition of the HHV-6B tetramer in infected and transfected cells. Biolayer interferometry (BLI) demonstrated that these antibodies exhibited dissociation constants (KD) in the range of 62.52–76.99 nM. We next assessed the effect of antibodies on the interaction between the HHV-6B tetramer and CD134. None of the gH-targeting antibodies inhibited the CD134 binding. Competition analysis using BLI-based antibody-Fab competition assays indicated that gHgL J1, gHgL O2, and gHgL F5 form a competition-defined antigenic cluster distinct from that recognized by OHV-3. Collectively, these findings identify a group of anti-gH antibodies which neutralizes HHV-6B with CD134-binding-independent profiles and suggest the presence of at least two distinguishable antibody-recognition regions within HHV-6B gH. These results expand our understanding of the antigenic organization of the HHV-6B entry complex.\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 9","pages":"448-461"},"PeriodicalIF":1.6,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148471771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune Responses to Live-Attenuated Prime-Boost Vaccination Are Independent of Injection Site in Calves 小牛对减毒活疫苗的免疫反应与注射部位无关。

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-09-08 Epub Date: 2026-07-17 DOI: 10.1111/1348-0421.70079

Mari Ikehata, Tomohiro Okagawa, Hayato Nakamura, Maho Inoue, Naoya Maekawa, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi, Satoru Konnai

{"title":"Immune Responses to Live-Attenuated Prime-Boost Vaccination Are Independent of Injection Site in Calves","authors":"Mari Ikehata, Tomohiro Okagawa, Hayato Nakamura, Maho Inoue, Naoya Maekawa, Yasuhiko Suzuki, Shiro Murata, Kazuhiko Ohashi, Satoru Konnai","doi":"10.1111/1348-0421.70079","DOIUrl":"10.1111/1348-0421.70079","url":null,"abstract":"<div>\u0000 \u0000 Vaccination is highly effective in controlling infectious diseases in calves. A prime-boost injection is often administered intramuscularly in the neck and the hip in calves. However, no guidelines have established whether the same vaccination sites could be used for booster vaccinations. This study investigated the influence of intramuscular administration sites for sequential vaccines on immune responses in calves. All calves received the primary live-attenuated viral vaccine into the left side of the neck. Four weeks later, the booster was administered at the same site as the primary vaccine (local boost group) or in the right hip (distal boost group). The neutralizing antibody titers did not differ significantly between the groups. To assess T-cell responses to viral antigens after booster vaccination, the expression levels of lymphocyte activation markers CD25 and CD69 were measured in peripheral blood mononuclear cells (PBMCs). The site of booster administration had no significant effect on the activation of CD4+ and CD8+ T cells. Furthermore, vaccine-induced production of interferon-γ, tumor necrosis factor-α, and interleukin-6 in PBMC culture supernatants did not differ significantly between the groups. This study detected no significant differences in vaccine-specific immune responses between the local and distal booster vaccination sites in calves.\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 9","pages":"427-435"},"PeriodicalIF":1.6,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148471760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ER-Resident Protein PDZD8 Inhibits Virus Production of West Nile Virus 内质网驻留蛋白PDZD8对西尼罗病毒产生的抑制作用

IF 1.6 4区医学

Microbiology and Immunology Pub Date : 2026-09-08 Epub Date: 2026-06-29 DOI: 10.1111/1348-0421.70072

Shiori Abe, Akari Sato, Haruto Eguchi, Keisuke Maezono, Passawat Thammahakin, Duong Thi Ngoc Thuy, Hiroaki Kariwa, Shintaro Kobayashi

引用次数: 0