Microbiology and Immunology最新文献_第2页

Elevated Level of miR-711 Is Associated With Inflammation and Disease Progression in Patients With Severe Acute Pancreatitis miR-711水平升高与严重急性胰腺炎患者的炎症和疾病进展相关

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-04-07 Epub Date: 2026-01-28 DOI: 10.1111/1348-0421.70044

Huili Chen, Changjing Qu, Xiufeng Pang, Xuan Wang, Ju Tian, Zhihua Tao, Feng Zhu

{"title":"Elevated Level of miR-711 Is Associated With Inflammation and Disease Progression in Patients With Severe Acute Pancreatitis","authors":"Huili Chen, Changjing Qu, Xiufeng Pang, Xuan Wang, Ju Tian, Zhihua Tao, Feng Zhu","doi":"10.1111/1348-0421.70044","DOIUrl":"10.1111/1348-0421.70044","url":null,"abstract":"<div>\u0000 \u0000 Severe acute pancreatitis (SAP) is a critical abdominal emergency, with inflammatory response being the core pathogenic mechanism. This study aims to explore the role of miR-711 in SAP and its underlying mechanisms. This study enrolled 121 MAP patients and 109 SAP patients, collecting their clinical data. qRT-PCR measured miR-711 expression in serum and cells. Cell viability was tested with CCK-8, inflammatory factors via ELISA kits, and oxidative stress using SOD and MDA kits. The dual-luciferase assay confirmed miR-711 and KRT8 binding. Compared to the mild acute pancreatitis (MAP) group, the expression level of miR-711 in the serum of patients in the SAP group was significantly upregulated and positively correlated with relevant indicators. The ROC curve demonstrated its potential diagnostic value for SAP. In vitro, the miR-711 inhibitor effectively enhances the viability of Caerulein-induced AR42J cells and alleviates their inflammation and oxidative damage. Furthermore, the study confirmed that KRT8 is a downstream target gene of miR-711, and there is a negative correlation between their expressions. KRT8 may reverse the protective effect of miR-711 inhibitor on Cerulein-induced AR42J cells by regulating the NF-κB and p38 MAPK signaling pathways. The serum level of miR-711 in SAP patients is significantly upregulated and negatively correlated with SAP disease-related indicators. In vitro models demonstrate that inhibition of miR-711 effectively alleviates cerulein-induced cellular damage. Overexpression of KRT8 can reverse the cellular damage caused by miR-711 overexpression.</div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 4","pages":"222-231"},"PeriodicalIF":1.8,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146064297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Integrated Computational and In Vivo Assessment of a Novel Multi-Epitope Vaccine Against Dengue Virus Serotype 4 Capsid Protein 针对登革热病毒血清型4衣壳蛋白的新型多表位疫苗的综合计算和体内评估

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-04-07 Epub Date: 2026-02-12 DOI: 10.1111/1348-0421.70048

Elham Mohammed Khatrawi, Syed Luqman Ali, Rasha Alonaizan, Syed Mudasser Ali, Muhammad Kashif Obaid

{"title":"Integrated Computational and In Vivo Assessment of a Novel Multi-Epitope Vaccine Against Dengue Virus Serotype 4 Capsid Protein","authors":"Elham Mohammed Khatrawi, Syed Luqman Ali, Rasha Alonaizan, Syed Mudasser Ali, Muhammad Kashif Obaid","doi":"10.1111/1348-0421.70048","DOIUrl":"10.1111/1348-0421.70048","url":null,"abstract":"<div>\u0000 \u0000 Dengue Virus Serotype 4 (DENV4) continues to contribute substantially to global dengue morbidity, yet current tetravalent vaccines provide inconsistent protection and may pose risks of antibody-dependent enhancement (ADE). This study aimed to design and evaluate a serotype-specific multi-epitope vaccine (MEV-DV4) targeting the conserved capsid protein of DENV4 using integrated reverse vaccinology and immunoinformatics approaches. Conserved, antigenic, non-allergenic, and non-toxic B-cell, cytotoxic T lymphocyte (CTL), and helper T lymphocyte (HTL) epitopes were identified and assembled with a β-defensin adjuvant and PADRE sequence using optimized linkers. The final construct demonstrated high antigenicity (0.8559), structural stability, favorable physicochemical properties, and excellent solubility. Structural validation confirmed 97.6% of residues in favored Ramachandran regions. Molecular docking revealed strong interactions with TLR4 and TLR8, particularly MEV–TLR8, and molecular dynamics simulations supported the overall stability of the complex. Immune simulations predicted robust humoral and cellular responses with strong memory formation. Experimental validation in an albino mouse model using an alum-adjuvanted MEV-DV4 formulation showed early and potent antibody responses, with peak HI titers at day 21. Notably, antibody titers induced by MEV-DV4 were statistically comparable to those produced by a commercial inactivated dengue vaccine at all tested time points (p > 0.05), while no adverse reactions were observed. These computational and experimental findings demonstrate that MEV-DV4 is a safe, immunogenic, and promising serotype-specific vaccine candidate against DENV4. Further neutralization and challenge studies are warranted to advance its preclinical development.</div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 4","pages":"241-258"},"PeriodicalIF":1.8,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Detection of a Novel Parahenipavirus From Northern Short-Tailed Shrews (Blarina brevicauda [Say, 1823]) 北方短尾鼩（Blarina brevicauda）中一种新型旁希尼帕病毒的检测[Say， 1823]。

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-04-07 Epub Date: 2026-01-23 DOI: 10.1111/1348-0421.70043

Sakiho Imai, Mai Kishimoto, Masayuki Horie

{"title":"Detection of a Novel Parahenipavirus From Northern Short-Tailed Shrews (Blarina brevicauda [Say, 1823])","authors":"Sakiho Imai, Mai Kishimoto, Masayuki Horie","doi":"10.1111/1348-0421.70043","DOIUrl":"10.1111/1348-0421.70043","url":null,"abstract":"<div>\u0000 \u0000 Parahenipaviruses (genus Parahenipavirus) are nonsegmented negative-strand RNA viruses belonging to the family Paramyxoviridae of the order Mononegavirales. Parahenipaviruses have been detected in shrews and rodents, some of which have been reported to be associated with human diseases. Although many parahenipaviruses have been identified thus far, there still exist large phylogenetic gaps in parahenipaviruses, suggesting the existence of yet-to-be-identified parahenipaviruses. In this study, we analyzed public RNA-seq data and identified a novel parahenipavirus from northern short-tailed shrews (Blarina brevicauda [Say, 1823]), tentatively named Blarina brevicauda virus (BbV). Based on sequence comparisons between BbV and its most closely related viruses, BbV meets the International Committee on Taxonomy of Viruses species demarcation criteria, and therefore we propose that BbV is a novel species of virus in the genus Parahenipavirus. Furthermore, mapping analysis using RNA-seq data derived from multiple tissues of northern short-tailed shrews suggested a possible kidney tropism of BbV. This study provides novel insights into the diversity of parahenipaviruses, which would contribute to a deeper understanding of their evolution and control of infectious diseases.\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 4","pages":"214-221"},"PeriodicalIF":1.8,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Issue Information – Cover 发行资料-封面

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-04-07 DOI: 10.1111/1348-0421.70055

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Inhibition of Targeted Bacterial Growth Using MazF-Expressing Phagemids: A Novel Therapeutic Approach Based on Endoribonuclease Toxin Molecules 利用表达mazf的噬菌体抑制靶向细菌生长：一种基于核糖核酸内切酶毒素分子的新治疗方法。

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-04-07 Epub Date: 2026-02-04 DOI: 10.1111/1348-0421.70046

Hana Hasegawa, Tatsuki Miyamoto, Rino Isshiki, Tomoyoshi Kaneko, Kenichi Takasugi, Naohiro Noda, Satoshi Tsuneda

{"title":"Inhibition of Targeted Bacterial Growth Using MazF-Expressing Phagemids: A Novel Therapeutic Approach Based on Endoribonuclease Toxin Molecules","authors":"Hana Hasegawa, Tatsuki Miyamoto, Rino Isshiki, Tomoyoshi Kaneko, Kenichi Takasugi, Naohiro Noda, Satoshi Tsuneda","doi":"10.1111/1348-0421.70046","DOIUrl":"10.1111/1348-0421.70046","url":null,"abstract":"The extensive and indiscriminate use of antibiotics, which are intended to halt bacterial growth or eliminate them, has unintentionally given rise to antimicrobial resistance (AMR), which is a critical threat to global health. In the pursuit of alternative solutions, researchers have focused on type II toxin–antitoxin systems prevalent within the bacterial domain. One such promising example is the MazF toxin, which has been demonstrated to cleave RNA at specific sequences. MazF toxins are now recognized as present in a diverse range of bacterial species and exhibit varying cleavage sequence specificities. This diversity offers the potential for controlling bacterial growth by silencing the expression of hundreds of essential genes. In this study, a preliminary screening of five MazF homologs from distinct taxonomic groups was conducted to identify a MazF candidate that can effectively suppress bacterial proliferation. MazFne1, an endoribonuclease toxin originating from Nitrosomonas europaea that specifically targets UGG sequences, emerged as a promising candidate. Building upon this discovery, a phagemid system was engineered to deliver mazFne1 and express it in two distinct Escherichia coli strains. This approach successfully inhibited E. coli growth, suggesting a potential strategy for developing therapeutic interventions to combat antibiotic-resistant bacterial infections.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 4","pages":"206-213"},"PeriodicalIF":1.8,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13058170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146119574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Overexpression of Escherichia coli yaiX Confers Multidrug Resistance and Enhances Virulence in the Silkworm Infection Model 家蚕感染模型中大肠埃希菌yaiX的过表达赋予多药耐药并增强毒力

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-04-07 Epub Date: 2026-02-25 DOI: 10.1111/1348-0421.70049

Kinuka Hongu, Kazuya Ishikawa, Tomoki Kosaki, Shin-Ichi Miyoshi, Kazuyuki Furuta, Chikara Kaito

{"title":"Overexpression of Escherichia coli yaiX Confers Multidrug Resistance and Enhances Virulence in the Silkworm Infection Model","authors":"Kinuka Hongu, Kazuya Ishikawa, Tomoki Kosaki, Shin-Ichi Miyoshi, Kazuyuki Furuta, Chikara Kaito","doi":"10.1111/1348-0421.70049","DOIUrl":"10.1111/1348-0421.70049","url":null,"abstract":"The emergence of bacteria with both antimicrobial resistance and high virulence has become a global health concern, underscoring the urgent need to elucidate the molecular basis underlying these traits. Here, we employed the silkworm (Bombyx mori) infection model, which is suitable for high-throughput screening, together with an Escherichia coli library containing plasmid clones of all genes from strain W3110, to identify genes whose overexpression enhances virulence. We found that overexpression of the uncharacterized protein YaiX promoted bacterial proliferation in silkworms and increased host lethality. Compared with the empty-vector control, the YaiX-overexpressing strain exhibited resistance to multiple antimicrobial agents with diverse mechanisms of action, including β-lactams, tetracyclines, fluoroquinolones, aminoglycosides, cationic surfactants, and hydrogen peroxide. Sequence analysis revealed that amino acids 18–52 of YaiX contain a transferase hexapeptide domain predicted to form a left-handed parallel β-helix. Overexpression of YaiX mutants lacking regions outside this domain conferred ampicillin resistance, whereas deletion of the hexapeptide domain abolished this phenotype. RNA sequencing and GO enrichment analyses further indicated that YaiX overexpression altered the expression of genes encoding RNA-binding proteins and porins. These findings suggest that YaiX overexpression, through its hexapeptide domain, modulates gene expression and contributes to both multidrug resistance and enhanced virulence in E. coli.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 4","pages":"193-205"},"PeriodicalIF":1.8,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13058169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147284423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Synergistic Activation of NO and Cytokine Production by TLR3 With TLR5 or TLR7 Agonists in RAW 264.7 Cells TLR3与TLR5或TLR7激动剂在RAW 264.7细胞中协同激活NO和细胞因子产生

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-04-07 Epub Date: 2026-02-10 DOI: 10.1111/1348-0421.70045

Thu-Dung Doan, Haroon Afzal, Asad Murtaza, Kamonpun Chuekwon, Li-Ting Cheng

{"title":"Synergistic Activation of NO and Cytokine Production by TLR3 With TLR5 or TLR7 Agonists in RAW 264.7 Cells","authors":"Thu-Dung Doan, Haroon Afzal, Asad Murtaza, Kamonpun Chuekwon, Li-Ting Cheng","doi":"10.1111/1348-0421.70045","DOIUrl":"10.1111/1348-0421.70045","url":null,"abstract":"<div>\u0000 \u0000 Macrophages are key components of the innate immune system, recognizing pathogen-associated molecular patterns (PAMPs) via Toll-like receptors (TLRs) to initiate immune responses. This study investigated the individual and combinatorial effects of TLR3 Poly(I:C), TLR5 (Flagellin), and TLR7 (Imiquimod) ligands on nitric oxide (NO) production and pro-inflammatory cytokine expression in RAW 264.7 mouse macrophage cells. Our results demonstrate that all three individual TLR agonists induced NO production and cytokine expression. Notably, co-stimulation with Poly(I:C) and imiquimod led to a significant synergistic enhancement of NO production, particularly at lower concentrations. A robust upregulation was observed in key Th1-type (IL-12p40, IFN-γ, TNF-α) and Th2-type (IL-6) cytokines. The optimal synergistic response for cytokine induction was observed with a 0.1 µg/mL Poly(I:C) and 1 µg/mL imiquimod combination. These findings highlight a potent crosstalk between TRIF-dependent (TLR3) and MyD88-dependent (TLR7, TLR5) signaling pathways, leading to amplified immune activation. Our study highlights the potential of synergistic TLR ligand combinations as powerful immunomodulators, offering promising avenues for the rational design of more effective vaccine adjuvants and innovative strategies in cancer immunotherapy.</div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 4","pages":"232-240"},"PeriodicalIF":1.8,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146150391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Microbiota in Green Tea Beverages Remaining in Plastic Bottles After Direct Drinking 绿茶饮料直接饮用后在塑料瓶中残留的微生物群。

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-03-05 Epub Date: 2026-01-20 DOI: 10.1111/1348-0421.70041

Miho Kawachi, Anna Wakui, Manami Imai, Mirai Sekiguchi, Haruna Sato, Yuki Kato, Rika Okabe, Takashi Abe, Nanase Takahashi, Nagara Kaku, Misato Miyazawa, Aya Sato, Yuka Naruse, Nanami Kubo, Ayaka Misumi, Hiroto Sano, Yuki Abiko, Jumpei Washio, Kaori Tanaka, Nobuhiro Takahashi, Takuichi Sato

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Issue Information – Cover 发行资料-封面

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-03-05 DOI: 10.1111/1348-0421.70050

引用次数: 0

Strain Traits of Intracranially Administered L-Type Bovine Spongiform Encephalopathy Prions Are not Significantly Modified During Intraspecies Transmission in Cynomolgus Monkeys 经脑内注射的l型牛海绵状脑病朊病毒在食蟹猴种内传播过程中没有明显的毒株性状改变。

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2026-03-05 Epub Date: 2026-01-19 DOI: 10.1111/1348-0421.70040

Ken'ichi Hagiwara, Hiroaki Shibata, Minoru Tobiume, Yuko Sato, Keiko Ohto, Sachi Okabayashi, Nozomi Nakano, Motohiro Horiuchi, Fumiko Ono

{"title":"Strain Traits of Intracranially Administered L-Type Bovine Spongiform Encephalopathy Prions Are not Significantly Modified During Intraspecies Transmission in Cynomolgus Monkeys","authors":"Ken'ichi Hagiwara, Hiroaki Shibata, Minoru Tobiume, Yuko Sato, Keiko Ohto, Sachi Okabayashi, Nozomi Nakano, Motohiro Horiuchi, Fumiko Ono","doi":"10.1111/1348-0421.70040","DOIUrl":"10.1111/1348-0421.70040","url":null,"abstract":"Among the three prion strains of bovine spongiform encephalopathy (BSE), classical BSE (C-BSE) prions are known causative agents of variant Creutzfeldt–Jakob disease. By contrast, human infections with L-type (L-) or H-type (H-) BSE prions have not been reported. Nonetheless, the zoonotic potential of L-BSE prions is supported by their successful primary transmission from cattle to cynomolgus macaque (Macaca fascicularis) monkeys via intracranial challenge. To assess whether the defining strain traits of L-BSE prions remain stable following secondary intraspecies transmission, we prepared brain homogenates from a diseased macaque that had previously undergone primary transmission of L-BSE prions, and intracranially administered them into two naïve macaques. Both animals succumbed to the disease within humane endpoints comparable to those observed in the primary transmission. Histopathological and immunohistochemical analyses of brain tissues showed no significant changes relative to primary transmission, including severe vacuolation and fine synaptic distribution of disease-associated forms of prion protein (PrPSc) in the cerebrum, and sparse PrPSc plaques in the cerebellum. In bioassays using C57BL/6 J mice, cattle-derived L-BSE prions and those passaged once or twice in macaques failed to transmit to mice, whereas cattle-derived C-BSE prions and their macaque-passaged counterparts were transmissible. These findings refine our understanding of L-BSE pathogenesis and confirm the stability of L-BSE prions following intracranial transmission in a nonhuman primate model.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 3","pages":"166-174"},"PeriodicalIF":1.8,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12963689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0