Microbiology and Immunology最新文献_第8页

Distinct 5′ UTR Requirements for Translation of the Bicistronic X/P mRNA Among Avian Orthobornaviruses 禽原带病毒双链X/P mRNA翻译的不同5' UTR要求

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2025-12-07 DOI: 10.1111/1348-0421.70031

Meng-Chi Wu, Takehiro Kanda, Ryo Komorizono, Madoka Sakai, Alexander Leacy, Leonardo Susta, Akiko Makino, Keizo Tomonaga

{"title":"Distinct 5′ UTR Requirements for Translation of the Bicistronic X/P mRNA Among Avian Orthobornaviruses","authors":"Meng-Chi Wu, Takehiro Kanda, Ryo Komorizono, Madoka Sakai, Alexander Leacy, Leonardo Susta, Akiko Makino, Keizo Tomonaga","doi":"10.1111/1348-0421.70031","DOIUrl":"10.1111/1348-0421.70031","url":null,"abstract":"Orthobornaviruses express X and the phosphoprotein (P) from a bicistronic X/P mRNA, and these proteins regulate polymerase activity. In mammalian orthobornaviruses, the 5′ untranslated region (5′ UTR) of the X/P mRNA controls the translational balance between X and P and thereby promotes efficient replication. Avian bornaviruses (ABVs) belong to two clades, clade-2 and -3, that differ in the structure and length of the 5′ UTR of the X/P mRNA. However, the functional consequences of these differences remain unclear. Using reverse genetics, we generated chimeric viruses by reciprocally exchanging the 5′ UTR of the X/P mRNA among clade 2 parrot bornavirus 5 (PaBV-5) and aquatic bird bornavirus 1 (ABBV-1) and clade 3 PaBV-4. In PaBV-5, a long 5′ UTR with a stem–loop and an upstream ORF was required to maintain the X-to-P translational balance. On the other hand, replacing the 5′ UTR of the X/P mRNA from PaBV-5 with that from PaBV-4 reduced X expression and markedly impaired viral growth. However, PaBV-4 tolerated the 5′ UTR of the X/P mRNA from PaBV-5 without detectable effects on translation or replication, which suggests that translation of PaBV-4 X/P mRNA does not depend on the origin of the 5′ UTR. Furthermore, ABBV-1 replicated efficiently with the 5′ UTR of the X/P mRNA from PaBV-5 but was strongly attenuated with that from PaBV-4. Taken together, these results demonstrate a clade-dependent requirement for the 5′ UTR for translation of the X/P mRNA and provide novel insights into the evolution of translational control in orthobornaviruses.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 2","pages":"80-90"},"PeriodicalIF":1.8,"publicationDate":"2025-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12868937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145701070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Bordetella BcrH1 and BcrH2 Are Specific Chaperones for the Pore-Forming Complex 博德氏菌BcrH1和BcrH2是孔隙形成复合物的特异性伴侣。

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2025-12-07 DOI: 10.1111/1348-0421.70030

Yuya Kishino, Toshinobu Ogawa, Hiroko Sato, Akio Abe, Asaomi Kuwae

{"title":"Bordetella BcrH1 and BcrH2 Are Specific Chaperones for the Pore-Forming Complex","authors":"Yuya Kishino, Toshinobu Ogawa, Hiroko Sato, Akio Abe, Asaomi Kuwae","doi":"10.1111/1348-0421.70030","DOIUrl":"10.1111/1348-0421.70030","url":null,"abstract":"Bordetella has a type III secretion system that secretes virulence proteins crucial to the establishment of infection. The genes encoding components of the Bordetella type III secretion system are located in the bsc region on the chromosome. This region includes the bcrH1 and bcrH2 genes, which respectively encode the proteins BcrH1 and BcrH2. In this study, we analyzed the functions of BcrH1 and BcrH2 in the Bordetella type III secretion system. First, we created a BcrH1-deficient strain and a BcrH2-deficient strain. We analyzed the amounts of the type III secreted proteins BopB and BopD, which make a complex that forms pores in the host membrane, in bacterial cells of each protein-deficient strain. The results showed that the BopB and BopD signals were weakened in the whole cell fraction of the BcrH1-deficient strain and the BcrH2-deficient strain, respectively. The hemolytic activity and cell toxicity of each BcrH protein-deficient strain were significantly lower than those of the wild-type strain. When anti-BcrH1 and anti-BcrH2 antibodies were used for the immunoprecipitation assay, the BopB and BopD signals were detected in the precipitated fractions, respectively. These results strongly suggest that BcrH1 and BcrH2 are specific chaperones for maintaining the stability of BopB and BopD, respectively.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 2","pages":"57-67"},"PeriodicalIF":1.8,"publicationDate":"2025-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12868935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145701021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Recovery of Infectious Oz Virus From Cloned cDNA 从克隆cDNA中恢复感染性奥兹病毒。

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2025-12-03 DOI: 10.1111/1348-0421.70029

Tofazzal Md Rakib, Lipi Akter, Hiroshi Shimoda, Yudai Kuroda, Hiromichi Matsugo, Shuzo Urata, Yusuke Matsumoto

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Issue Information – Cover 发行资料-封面

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2025-12-02 DOI: 10.1111/1348-0421.70028

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Hydroxypropyl Cellulose Enhances Immune Responses to the Current Seasonal Influenza Vaccine in Mice 羟丙基纤维素增强小鼠对当前季节性流感疫苗的免疫反应

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2025-12-01 DOI: 10.1111/1348-0421.70026

Nantaporn Kaewaroon, Sara Yoshimoto, Luthfi Muawan, Shintaro Shichinohe, Tokiko Watanabe

{"title":"Hydroxypropyl Cellulose Enhances Immune Responses to the Current Seasonal Influenza Vaccine in Mice","authors":"Nantaporn Kaewaroon, Sara Yoshimoto, Luthfi Muawan, Shintaro Shichinohe, Tokiko Watanabe","doi":"10.1111/1348-0421.70026","DOIUrl":"10.1111/1348-0421.70026","url":null,"abstract":"Influenza A and B viruses cause annual epidemics and continue to pose global public health concerns. The most effective approach to preventing or mitigating the severity of influenza is vaccination. Inactivated split influenza HA vaccines are commonly used worldwide due to their strong safety profile and broad range of target groups; however, their efficacy is suboptimal, especially in the elderly. Adjuvants are used to enhance the effectiveness of some influenza vaccines, but few adjuvants have been approved for human vaccines. Previously, we identified hydroxypropyl cellulose as a promising adjuvant for the split influenza HA vaccine for the 2015–2016 and 2016–2017 influenza seasons. Here, we evaluated whether hydroxypropyl cellulose could enhance the efficacy of the quadrivalent split HA vaccine for the 2023–2024 influenza season, which contains the HA proteins of A/Victoria/4897/2022 (H1N1)pdm09 and three other strains. Using a mouse model, we performed immunogenicity studies and assessed protective efficacy against challenges with homologous and heterologous H1N1pdm09 virus strains. We found that hydroxypropyl cellulose in combination with the HA vaccine generated higher virus-specific IgG antibody titers compared to the vaccine alone. The adjuvanted vaccine provided complete protection against homologous challenge and enhanced viral clearance from respiratory organs. Notably, the adjuvanted vaccine demonstrated cross-protective efficacy against heterologous H1N1pdm09 virus challenge, improving survival rates compared to vaccine alone. Our results demonstrate that hydroxypropyl cellulose has potential as an adjuvant for current seasonal influenza vaccines.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 2","pages":"68-79"},"PeriodicalIF":1.8,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12868939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145653282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Immunostimulatory Activity of Solubilized Peptidoglycan Derivatives Prepared From Lactic Acid Bacteria 乳酸菌制备的可溶性肽聚糖衍生物的免疫刺激活性。

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2025-11-26 DOI: 10.1111/1348-0421.70025

Sho Noguchi, Momoko Nakayama, Masahiro Eguchi, Sakura Onoue, Hiroaki Kouzai, Kazuyoshi Kawahara

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OmpA_C-Like Domain of PorE Is Essential for PorE Function in the Type IX Secretion System (T9SS) of Porphyromonas gingivalis and Some T9SS Cargo Proteins Are Secreted in a PorE-Independent Manner 在牙龈卟啉单胞菌IX型分泌系统（T9SS）中，孔隙的OmpA_C-Like结构域对孔隙功能至关重要，一些T9SS货物蛋白以不依赖于孔隙的方式分泌。

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2025-11-19 DOI: 10.1111/1348-0421.70023

Takashi Tominaga, Hideharu Yukitake, Paul D. Veith, Dhana G. Gorasia, Takao Ayuse, Takuro Sanuki, Eric C. Reynolds, Koji Nakayama, Mariko Naito, Mikio Shoji

{"title":"OmpA_C-Like Domain of PorE Is Essential for PorE Function in the Type IX Secretion System (T9SS) of Porphyromonas gingivalis and Some T9SS Cargo Proteins Are Secreted in a PorE-Independent Manner","authors":"Takashi Tominaga, Hideharu Yukitake, Paul D. Veith, Dhana G. Gorasia, Takao Ayuse, Takuro Sanuki, Eric C. Reynolds, Koji Nakayama, Mariko Naito, Mikio Shoji","doi":"10.1111/1348-0421.70023","DOIUrl":"10.1111/1348-0421.70023","url":null,"abstract":"The periodontal pathogen Porphyromonas gingivalis secretes gingipains, highly hydrolytic proteases, to the cell surface or external environment via a type IX secretion system (T9SS). PorE is one of the T9SS component proteins essential for gingipain secretion. It consists of four domains: TPR, WD40, CRD, and OmpA_C-like. In this study, we investigated the contribution and function of these PorE domains to gingipain secretion by generating PorE mutants with domain deletions and residue substitutions. We found that (i) the OmpA_C-like domain is essential for gingipain secretion, (ii) the peptidoglycan binding sites (Asp576 and Asn584) in the OmpA_C-like domain are a necessity for gingipain secretion, (iii) the WD40 domain is likely involved in interaction with PorP, and (iv) the CRD domain is not essential for gingipain secretion. The function of the TPR domain could not be assessed because no PorE protein of the predicted molecular mass was detected in the TPR domain-deleted PorE mutant. We also demonstrated that some T9SS cargo proteins, such as PorA and Hbp35, do not require the PorE-PorP complex for secretion, suggesting that the PorE–PorP complex may occupy a unique position within the T9SS that is distinct from other components.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 1","pages":"15-26"},"PeriodicalIF":1.8,"publicationDate":"2025-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.70023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145557357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Development of a Porcine Cell Line Stably Expressing Ephrin-B2 for Nipah Virus Research and Diagnostic Testing 猪尼帕病毒稳定表达Ephrin-B2细胞系的建立及诊断试验研究。

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2025-11-17 DOI: 10.1111/1348-0421.70022

Hui Zhang, Akatsuki Saito

{"title":"Development of a Porcine Cell Line Stably Expressing Ephrin-B2 for Nipah Virus Research and Diagnostic Testing","authors":"Hui Zhang, Akatsuki Saito","doi":"10.1111/1348-0421.70022","DOIUrl":"10.1111/1348-0421.70022","url":null,"abstract":"Nipah virus (NiV) is a highly pathogenic zoonotic virus transmitted from bats to humans through pigs as a crucial intermediate host. NiV outbreaks pose significant public health and economic threats, especially for pig farmers. Although the World Organization for Animal Health recommends African green monkey-derived Vero cells for NiV isolation, pig-derived cell lines could represent an optimal platform for propagating pig-origin NiV, as signs and symptoms of NiV infection differ among different hosts. In this study, we generated and evaluated pig-derived PK-15 cells stably expressing pig-derived ephrin-B2 (PK-15/Ephrin-B2 cells), the primary receptor for NiV. NiV pseudovirus infectivity was increased by > 1000-fold in PK-15/Ephrin-B2 cells compared with that in wild-type PK-15 cells, whereas virus susceptibility was higher in PK-15/Ephrin-B2 cells than in Vero cells (> 30-fold). Furthermore, Stat2-knockout PK-15/Ephrin-B2 cells exhibited stable viral infectivity in the presence of type I interferon, making it particularly suitable for clinical sample testing. Moreover, PK-15/Ephrin-B2 cells proved useful for neutralization tests using anti-NiV hyperimmune ascitic fluid. Therefore, PK-15 cells expressing pig ephrin-B2 could represent an efficient tool for virus isolation, vaccine development, and virological studies of NiV and related henipaviruses.","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 1","pages":"36-46"},"PeriodicalIF":1.8,"publicationDate":"2025-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1348-0421.70022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Correction to “Bifidobacterium Bifidum G9-1 Survives In the Intestinal Environment and Influences the Gut Microbiota Despite the Presence of Antimicrobials” 更正“两歧双歧杆菌G9-1在肠道环境中存活并影响肠道微生物群，尽管存在抗菌剂”。

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2025-11-17 DOI: 10.1111/1348-0421.70024

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Administration of Lactiplantibacillus brownii Strain MH-1 During the Larval Stage Increases the Resistance Against Staphylococcus aureus subsp. aureus in Caenorhabditis elegans 在幼虫期施用布朗氏乳杆菌MH-1增强对金黄色葡萄球菌的抗性。秀丽隐杆线虫中的金黄色葡萄球菌。

IF 1.8 4区医学

Microbiology and Immunology Pub Date : 2025-11-03 DOI: 10.1111/1348-0421.70020

Mina Hashimoto, Yoshihiko Tanimoto, Eriko Kage-Nakadai

{"title":"Administration of Lactiplantibacillus brownii Strain MH-1 During the Larval Stage Increases the Resistance Against Staphylococcus aureus subsp. aureus in Caenorhabditis elegans","authors":"Mina Hashimoto, Yoshihiko Tanimoto, Eriko Kage-Nakadai","doi":"10.1111/1348-0421.70020","DOIUrl":"10.1111/1348-0421.70020","url":null,"abstract":"<div>\u0000 \u0000 Lactiplantibacillus brownii is a new species of lactic acid bacteria discovered in sauerkraut in Russia, first reported in 2023. We previously isolated L. brownii strain MH-1 (MH-1) from homemade pickled Chinese cabbage in Japan and reported that MH-1 prolonged healthspan and improved stress resistance in Caenorhabditis elegans. In the present study, we investigated the effect of feeding MH-1 on resistance to infections in C. elegans. MH-1 failed to increase its resistance to infection with Staphylococcus aureus subsp. aureus (SA) and Salmonella enterica (SE) at the adult stage. However, MH-1 feeding at the larval stage remarkably increased the survival rate of worms infected with SA, but not with SE. Genes related to the innate immune response were significantly upregulated by MH-1 feeding at the larval stage compared with those of the control group. A survival assay using loss-of-function mutants revealed that resistance to SA infection conferred by MH-1 feeding during the larval stage requires daf-16 and pmk-1. These results suggest that MH-1 feeding at the larval stage likely confers resistance to SA infection by enhancing host immunity via the insulin/insulin-like growth factor 1 signaling and p38 mitogen-activated protein kinase pathways.\u0000 </div>","PeriodicalId":18679,"journal":{"name":"Microbiology and Immunology","volume":"70 1","pages":"1-8"},"PeriodicalIF":1.8,"publicationDate":"2025-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145431899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0