生物膜衍生的膜囊泡对铜绿假单胞菌 PAO1 表现出强大的免疫调节活性。

IF 1.9 4区 医学 Q4 IMMUNOLOGY
Minato Takahara, Satoru Hirayama, Hiroyuki Futamata, Ryoma Nakao, Yosuke Tashiro
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引用次数: 0

摘要

致病细菌会在上皮细胞上形成生物膜,大多数细菌生物膜都会增加膜囊(MVs)的产生,革兰氏阴性细菌的膜囊也称为外膜囊。许多研究对浮游生物条件下释放的膜囊泡进行了调查,但生物膜释放的膜囊泡对免疫反应的影响仍不清楚。本研究旨在调查从铜绿假单胞菌 PAO1 的浮游生物和生物膜培养物中获得的 MVs 的特征和免疫调节活性。通过测量促炎细胞因子的 mRNA 表达,研究了巨噬细胞对浮游生物源 MVs(p-MVs)和生物膜源 MVs(b-MVs)的先天性免疫反应。结果显示,与 p-MVs 相比,b-MVs 诱导的炎性细胞因子(包括 Il1b、Il6 和 Il12p40)表达量更高。两种中毒性病毒的 Toll 样受体 4(Tlr4)的 mRNA 表达水平不同,但 Tlr2 的表达水平没有差异。多粘菌素 B 能明显中和 b-MV 介导的细胞因子诱导,这表明原生 b-MV 的脂多糖是免疫反应的起源。此外,热处理或均质化的b-MV可诱导细胞因子(包括Tnfa、Il1b、Il6和Il12p40)的mRNA表达。热处理中毒性细胞可导致 Tlr2 的表达增加,但 Tlr4 的表达却没有增加,这表明 TLR2 配体在检测裂解中毒性细胞中的病原体相关分子模式方面发挥了作用。总之,我们的数据表明,铜绿微囊藻生物膜中会产生强效的免疫调节MV,这种行为可能是细菌感染宿主细胞的一种策略。此外,我们的研究结果将有助于利用 MVs 开发新型疫苗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Biofilm-derived membrane vesicles exhibit potent immunomodulatory activity in Pseudomonas aeruginosa PAO1

Biofilm-derived membrane vesicles exhibit potent immunomodulatory activity in Pseudomonas aeruginosa PAO1

Pathogenic bacteria form biofilms on epithelial cells, and most bacterial biofilms show increased production of membrane vesicles (MVs), also known as outer membrane vesicles in Gram-negative bacteria. Numerous studies have investigated the MVs released under planktonic conditions; however, the impact of MVs released from biofilms on immune responses remains unclear. This study aimed to investigate the characteristics and immunomodulatory activity of MVs obtained from both planktonic and biofilm cultures of Pseudomonas aeruginosa PAO1. The innate immune responses of macrophages to planktonic-derived MVs (p-MVs) and biofilm-derived MVs (b-MVs) were investigated by measuring the mRNA expression of proinflammatory cytokines. Our results showed that b-MVs induced a higher expression of inflammatory cytokines, including Il1b, Il6, and Il12p40, than p-MVs. The mRNA expression levels of Toll-like receptor 4 (Tlr4) differed between the two types of MVs, but not Tlr2. Polymyxin B significantly neutralized b-MV-mediated cytokine induction, suggesting that lipopolysaccharide of native b-MVs is the origin of the immune response. In addition, heat-treated or homogenized b-MVs induced the mRNA expression of cytokines, including Tnfa, Il1b, Il6, and Il12p40. Heat treatment of MVs led to increased expression of Tlr2 but not Tlr4, suggesting that TLR2 ligands play a role in detecting the pathogen-associated molecular patterns in lysed MVs. Taken together, our data indicate that potent immunomodulatory MVs are produced in P. aeruginosa biofilms and that this behavior could be a strategy for the bacteria to infect host cells. Furthermore, our findings would contribute to developing novel vaccines using MVs.

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来源期刊
Microbiology and Immunology
Microbiology and Immunology 医学-免疫学
CiteScore
5.20
自引率
3.80%
发文量
78
审稿时长
1 months
期刊介绍: Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.
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