Medical mycology最新文献_第5页

Pulmonary aspergillosis in green sea turtles (Chelonia mydas): A case series. 绿海龟（Chelonia mydas）肺曲霉菌病：绿海龟曲霉菌病病例系列。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-12-05 DOI: 10.1093/mmy/myae114

Vanice Rodrigues Poester, Jessica E Dávila Hidalgo, Lívia Silveira Munhoz, Mariana Rodrigues Trápaga, Emília Ferreira Andrade, Paula Lima Canabarro, Pamela Parmigiani, Eduarda Corrêa Freitas, Mariana Martins Flores, Éryca Ceolin Lamego, Andréa Corrado Adornes, Alessandro Comarú Pasqualotto, David A Stevens, Melissa Orzechowski Xavier

引用次数: 0

Association between preoperative antifungal therapy and postoperative complications in patients with pulmonary aspergilloma: A national database study in Japan. 肺曲菌瘤患者术前抗真菌治疗与术后并发症的关系：日本国家数据库研究

IF 2.7 3区医学

Medical mycology Pub Date : 2024-12-05 DOI: 10.1093/mmy/myae117

Keita Takeda, Akira Yokoyama, Takeshi Fukami, Yuya Kimura, Maho Suzukawa, Taisuke Jo, Junko Suzuki, Yuka Sasaki, Akihisa Mitani, Goh Tanaka, Asahi Fujita, Hiroki Matsui, Kiyohide Fushimi, Takahide Nagase, Hideo Yasunaga

{"title":"Association between preoperative antifungal therapy and postoperative complications in patients with pulmonary aspergilloma: A national database study in Japan.","authors":"Keita Takeda, Akira Yokoyama, Takeshi Fukami, Yuya Kimura, Maho Suzukawa, Taisuke Jo, Junko Suzuki, Yuka Sasaki, Akihisa Mitani, Goh Tanaka, Asahi Fujita, Hiroki Matsui, Kiyohide Fushimi, Takahide Nagase, Hideo Yasunaga","doi":"10.1093/mmy/myae117","DOIUrl":"10.1093/mmy/myae117","url":null,"abstract":"Postoperative complications of pulmonary resection in patients with pulmonary aspergilloma are common. Preoperative antifungal therapy is a perioperative management strategy to reduce postoperative complications; however, the benefit of the treatment remains controversial. A retrospective cohort study was conducted using data from the Diagnosis Procedure Combination database (July 2010-March 2021), a national inpatient database in Japan. Patients with pulmonary aspergilloma with or without chronic necrotizing pulmonary aspergillosis who underwent pulmonary resection were identified and divided into two groups based on whether they received preoperative antifungal therapy. Propensity-score overlap weighting was performed to compare the occurrence of composite postoperative complications and postoperative length of hospital stay between the two groups. We identified 98 patients with pulmonary aspergilloma who received preoperative antifungal therapy and 399 patients who did not. After propensity-score overlap weighting, the preoperative antifungal therapy group had a significantly lower proportion of composite postoperative complications than the non-preoperative antifungal therapy group (6.8% vs. 14.2%, P = .038). There was no significant difference in postoperative length of stay between the groups with and without preoperative antifungal therapy (median 14.6 vs. 15.8 days, P = .41). Preoperative antifungal therapy was associated with a reduction in postoperative complications in patients with pulmonary aspergilloma. Preoperative treatment with antifungals may be beneficial in reducing the risk of postoperative complications in patients with pulmonary aspergilloma undergoing pulmonary resection.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antemortem diagnostic tests for the detection of Aspergillus infection in birds: A systematic review. 用于检测鸟类曲霉菌感染的尸检诊断测试：系统综述。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-11-12 DOI: 10.1093/mmy/myae112

Sabrina Vieu, Jacques Guillot, François Beaudeau

{"title":"Antemortem diagnostic tests for the detection of Aspergillus infection in birds: A systematic review.","authors":"Sabrina Vieu, Jacques Guillot, François Beaudeau","doi":"10.1093/mmy/myae112","DOIUrl":"10.1093/mmy/myae112","url":null,"abstract":"Aspergillosis remains a common and life-threatening disease in captive and wild birds all over the world. The diagnosis is currently based on clinical signs or lesions, diagnostic imaging, and a variety of biological tests. This systematic review aimed to compare the accuracy of antemortem diagnostic tests for Aspergillus infection in birds. According to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, a search was conducted on PubMed, Scopus, Web of Science, and CAB until January 2024. The methodological quality was assessed with QUADAS 2 risk of bias tool. The 13 studies, selected for the review, included results from a wide variety of birds (mainly Spheniciformes but also Falconiformes, Psittaciformes, and Galliformes) from wildlife rehabilitation centers, zoological parks, or veterinary practices. Aspergillus infection was mainly confirmed by fungal culture and/or histopathology. Serum markers included Aspergillus components (galactomannan, β-d-glucan, mannoproteins, and gliotoxin), anti-Aspergillus antibodies, 3-hydroxybutyrate, as well as protein electrophoresis and acute phase molecules. Sensitivity and specificity displayed a large amount of variation despite threshold arrangement. Disparities in the number of individuals per study did not allow for reliable comparison. Platelia Ag Assay (Bio-Rad), the most commonly used test in the studies, demonstrated moderate specificity and low sensitivity. Overall, non-specific tests demonstrated more consistent performance, whereas specific tests showed greater variability. Based on current knowledge, none of these tests provide sufficient accuracy to reliably detect Aspergillus infection in birds in clinical practice.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geraniol inhibits both planktonic cells and biofilms of the Candida parapsilosis species complex: Highlight for the improved efficacy of amphotericin B, caspofungin and fluconazole plus Geraniol. 香叶醇可抑制副丝状念珠菌菌种群的浮游细胞和生物膜：两性霉素 B、卡泊芬净和氟康唑加香叶醇可提高疗效的亮点。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-11-12 DOI: 10.1093/mmy/myae105

José Júlio Costa Sidrim, Daniel Vieira Martins, Maria Gleiciane da Rocha, Géssica Dos Santos Araújo, Rossana de Aguiar Cordeiro, Glaucia Morgana de Melo Guedes, Waldemiro de Aquino Pereira-Neto, Débora de Souza Collares Maia Castelo-Branco, Marcos Fábio Gadelha Rocha

{"title":"Geraniol inhibits both planktonic cells and biofilms of the Candida parapsilosis species complex: Highlight for the improved efficacy of amphotericin B, caspofungin and fluconazole plus Geraniol.","authors":"José Júlio Costa Sidrim, Daniel Vieira Martins, Maria Gleiciane da Rocha, Géssica Dos Santos Araújo, Rossana de Aguiar Cordeiro, Glaucia Morgana de Melo Guedes, Waldemiro de Aquino Pereira-Neto, Débora de Souza Collares Maia Castelo-Branco, Marcos Fábio Gadelha Rocha","doi":"10.1093/mmy/myae105","DOIUrl":"10.1093/mmy/myae105","url":null,"abstract":"The Candida parapsilosis species complex poses a recognized threat to the nosocomial environment. In the scenario of the global rise of resistant strains to antifungals, geraniol, a terpene isolated from different essential oils, has shown promising antimicrobial activity. We evaluated: (1) the effects of geraniol against the C. parapsilosis species complex, in planktonic and biofilm forms; (2) the strains' susceptibility to clinical antifungals and (3) the geraniol interaction with antifungals. Eighteen isolates were subjected to in vitro susceptibility testing by the broth microdilution protocol, using geraniol, amphotericin B, caspofungin, itraconazole and fluconazole to determine the minimum inhibitory concentration (MIC) and subsequently, we measured the fungicidal activity. Geraniol was tested against biofilms by the measurement of the metabolic activity and biomass. Pharmacological interactions were performed by the checkerboard method. Geraniol's MIC range was between 256 and 512 µg/ml. MIC range for clinical antifungals was ≤ 0.031-4 µg/ml. Geraniol also showed antibiofilm activity with average reductions of metabolic activity (38.33%) and biomass (30.69%), at MIC concentration. Furthermore, geraniol showed synergistic/additive effects with antifungals. Briefly, geraniol inhibits both planktonic cells and biofilms of the C. parapsilosis species complex and besides it improves the efficacy of amphotericin B, caspofungin and fluconazole.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142546364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High histoplasmosis incidence in kidney transplant recipients in Santa Fe city, Argentina. 阿根廷圣菲市肾移植受者组织胞浆菌病发病率高。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-11-12 DOI: 10.1093/mmy/myae099

Manuela Loaiza-Oliva, Soledad Gamarra, Joaquín Agusti, Luis Gaite, José Hugo Paladini, Valeria Martínez, Mariano Arriola, Judith Ana Gaite, Guillermo Garcia-Effron

{"title":"High histoplasmosis incidence in kidney transplant recipients in Santa Fe city, Argentina.","authors":"Manuela Loaiza-Oliva, Soledad Gamarra, Joaquín Agusti, Luis Gaite, José Hugo Paladini, Valeria Martínez, Mariano Arriola, Judith Ana Gaite, Guillermo Garcia-Effron","doi":"10.1093/mmy/myae099","DOIUrl":"10.1093/mmy/myae099","url":null,"abstract":"Histoplasmosis is endemic in the central/northeast region of Argentina. It is estimated that the incidence of this mycosis is low in solid organ transplant recipients. This work aims to describe the epidemiology, clinical forms, and evolution of kidney transplant recipients diagnosed with histoplasmosis in Santa Fe city, Argentina. A retrospective study was carried out between 2015 and 2020 on kidney transplant patients with symptoms associated with histoplasmosis in Santa Fe. Histoplasmosis diagnosis was performed through histopathology, recovery of Histoplasma spp., by culture, and/or positive nested Polimerase Chain Reaction (PCR) specific for the Histoplasma Hc100 gene. During the study period, 360 kidney transplantations were performed. Of these patients, 12 were diagnosed with histoplasmosis (3.3%). The patients' median age was 51 years, and 75% were male. Eleven patients (92%) presented the disseminated form of the disease. Thirty-three percent were diagnosed with histoplasmosis in their first year post-transplantation (mostly 6-12 months), while 42% received their diagnosis 3 years after transplantation. Laboratory diagnosis was performed by histopathology, culture, and PCR in four cases (33%), by culture and PCR in three cases (25%), and by PCR alone in three cases (25%). Thus, all 12 patients showed positive nested PCR results. All patients received amphotericin B as initial treatment. A good response was observed in 83% of patients. We found a high incidence of histoplasmosis in kidney transplant recipients (up to 10 times higher than reports from other endemic areas). Diagnosis by histopathology/culture showed 75% sensitivity, while nested PCR showed better sensitivity and diagnostic speed.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142623848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Candida tropicalis morphotypes show altered cellular structure and gene expression pre- and post-exposure to fluconazole. 热带念珠菌形态在暴露于氟康唑前后显示出细胞结构和基因表达的改变。

IF 4.6 3区医学

Medical mycology Pub Date : 2024-11-12 DOI: 10.1093/mmy/myae110

Murilo M Dos Santos, Cássia M de Souza, Luciana Furlaneto-Maia, Marcia C Furlaneto

{"title":"Candida tropicalis morphotypes show altered cellular structure and gene expression pre- and post-exposure to fluconazole.","authors":"Murilo M Dos Santos, Cássia M de Souza, Luciana Furlaneto-Maia, Marcia C Furlaneto","doi":"10.1093/mmy/myae110","DOIUrl":"10.1093/mmy/myae110","url":null,"abstract":"A feature of Candida tropicalis is its ability to undergo phenotypic switching that can affect antifungal sensitivity and virulence traits. Here, we investigated the effect of switching on alterations at the cellular structure level of C. tropicalis morphotypes and whether exposure to fluconazole (FLC) in vitro could be associated with these alterations in a morphotype-dependent manner. Candida tropicalis morphotypes included clinical isolate (Parental) and two switch strains (Crepe variant and revertant of Crepe-RC). The minimum inhibitory concentration (MIC50) of fluconazole was determined according to EUCAST. Cell wall porosity, quantification of cell wall components, cell size/complexity, and expression of ERG11 and CDR1 genes in morphotypes pre- and post-exposure to fluconazole were determined. Crepe and RC showed an eightfold higher MIC50 (1 µg/ml) than the Parental (0.125 µg/ml). Exposure to FLC resulted in twofold higher MIC50 for Parental and RC. The Crepe variant exhibited a fourfold higher expression of ERG11, and the RC showed 10-fold higher expression of CDR1 than the clinical isolate. Switch strains showed reduced cell wall porosity compared to Parental, and exposure to FLC resulted in a significant reduction in the porosity of Parental and RC cells. Furthermore, phenotypic switching affected cell wall β-1,3-glucan and chitin contents in a morphotype-dependent manner. Our findings indicate that switching affects cellular structure in C. tropicalis and the occurrence of differential alterations between the clinical isolate and its switched states in response to fluconazole exposure.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fusarium spp. causing invasive disease in humans: A case series from north India. 引起人类侵袭性疾病的镰刀菌属：印度北部的系列病例。

IF 2.7 3区医学

Medical mycology Pub Date : 2024-11-12 DOI: 10.1093/mmy/myae111

Sudesh Gourav, Himanshu Mishra, Immaculata Xess, Ashu Seith Bhalla, Stuti Chandola, Sonakshi Gupta, Kavi Priya Appasami, Balaji Dattatraya Shukla, Sameer Bakhshi, Aish Manhas, Mragnayani Pandey, Bhaskar Rana, Gagandeep Singh

{"title":"Fusarium spp. causing invasive disease in humans: A case series from north India.","authors":"Sudesh Gourav, Himanshu Mishra, Immaculata Xess, Ashu Seith Bhalla, Stuti Chandola, Sonakshi Gupta, Kavi Priya Appasami, Balaji Dattatraya Shukla, Sameer Bakhshi, Aish Manhas, Mragnayani Pandey, Bhaskar Rana, Gagandeep Singh","doi":"10.1093/mmy/myae111","DOIUrl":"10.1093/mmy/myae111","url":null,"abstract":"Owing to their inherent resistance to different classes of antifungals, early identification of Fusarium spp. is crucial. In this study, 10 clinical isolates were included from patients with invasive fusariosis involving lungs, sinuses, or both. Clinico-radiological data were collected. Samples were processed by standard laboratory procedures. Three gene regions (ITS, TEF1, and RPB2) were amplified by PCR for multilocus sequencing. Fusarium MLST, FUSARIUM-ID, and FUSARIOID-ID databases were used for final identification. Antifungal susceptibility testing was performed by broth microdilution following CLSI M38-A3 and Sensititre™ YeastOne™ YO9 plate. Pulmonary involvement was seen in all patients, and sino-nasal involvement was present in six. Radiologically, consolidations and cavitations were present in eight and six cases, respectively. Halo sign was present in six; reverse halo sign was also found in three of them. Direct microscopy showed septate hyphae that were morphologically different from those found in aspergillosis. Results of the molecular identification were as follows: two Fusarium irregulare, one Fusarium pernambucanum, one Fusarium incarnatum, one Fusarium sp. FIESC 30, two Fusarium keratoplasticum, one Fusarium falciforme, one Fusarium pseudonygamai, and one Fusarium delphinoides. For both Fusarium solani (FSSC) and Fusarium incarnatum-equiseti (FIESC) species complexes, amphotericin B had the lowest minimum inhibitory concentrations (MICs). Importantly, for terbinafine, all FIESC isolates had low MICs, while FSSC isolates had high MICs. In some cases, early identification of Fusarium spp. is possible by means of morphology of hyphae on direct microscopy and findings on radiology. Molecular identification, at least to the species complex level, is crucial for the choice of antifungals.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Malassezia gallinae sp. nov., a new basidiomycetous yeast species isolated from skins of chickens. Malassezia gallinae sp.

IF 2.7 3区医学

Medical mycology Pub Date : 2024-11-12 DOI: 10.1093/mmy/myae109

Yu-Jing Zhao, Qin Ma, Miao-Miao Liu, Qi-Ming Wang

引用次数: 0

Routine laboratory screening for cyp51a-related azole resistance in Aspergillus fumigatus using Etest: Concordance with gene sequencing. 使用 Etest 对烟曲霉中与 CYP51A 相关的唑类抗药性进行常规实验室筛查：与基因测序的一致性。

IF 4.6 3区医学

Medical mycology Pub Date : 2024-11-12 DOI: 10.1093/mmy/myae107

Charlotte Rabault, Yann Rakotoarivony, Sandrine Houzé, Christine Bonnal, Valentin Joste

引用次数: 0

Current taxonomic status of the cultivable and uncultivable Paracoccidioides species. 可栽培和不可栽培 Paracoccidioides 物种的分类现状。