Medical mycology最新文献

{"title":"Epidemiological shifts of Invasive Fungal Infections in the current era of haematology.","authors":"Patricia Monzó-Gallo, Christian Teijon-Lumbreras, Tommaso Francesco Aiello, Antonio Gallardo-Pizarro, Ana Martinez-Urrea, Mariana Chumbita, Emmanuelle Gras, Olivier Peyrony, Marta Bodro, Laura Magnano, Sabina Herrera, Maria Suarez-Lledó, Mateu Espasa, Francesc Marco, Alex Soriano, Carolina Garcia-Vidal","doi":"10.1093/mmy/myaf070","DOIUrl":"https://doi.org/10.1093/mmy/myaf070","url":null,"abstract":"<p><p>We aim to describe the epidemiology and risk factors for invasive fungal infections (IFI) and invasive mold infections (IMI) in hospitalized hematologic patients within the context of current hematologic therapies. Retrospective observational cohort study conducted on consecutive hematologic patients admitted to a tertiary hospital (2020-2023). Two populations were analysed: the full cohort of hospitalized patients (FC) and the subset of patients for whom mycological testing was specifically requested to rule out an IFI (SC). Proven or probable IFI was classified using EORTC-MSG criteria. Risk factors for IFI and IMI were identified. A total of 1975 patients where included in the FC whereas 1154 were included in the SC. IFI was diagnosed in 64 patients (65 episodes), and IMI in 43 patients (44 episodes). Aspergillosis was the most common IFI (58.4%), followed by candidemia (18.5%), Pneumocystis jirovecii pneumonia (PJP) (15.4%), mucormycosis (6.2%), and fusariosis (4.6%). Independent risk factors for IFI in the FC included acute leukemia (aOR 2.40, 95% CI 1.37-4.10, p = 0.002), corticosteroid use (aOR 2.36, 95% CI 1.40-4.03, p = 0.001) and graft versus host disease (GVHD) (aOR 2.13, 95% CI, 0.93-4.46, p = 0.05). For IMI, risk factors were acute leukemia (aOR 2.71, 95% CI 1.33-5.52, p = 0.006), corticosteroid use (aOR 1.96, 95% CI 0.98-4-03, p = 0.05) and chronic lung disease (aOR 2.25, 95% CI 1.06-4.5, p = 0.02). In the SC, corticosteroid use (aOR 2.45, 95% CI 1.44-4.25, p = 0.001) was the independent risk factor for IFI and corticosteroid use (aOR 2.40, 95% CI 1.21-4.91, p = 0.01) and GVHD (aOR 2.95, 95% CI 1.23-6.52, p = 0.009) were independent factors associated with IMI. Mortality was significantly higher in IFI patients compared to non-IFI patients (51.6% vs. 20.3%, p < 0.001). In this new era of haematology, the epidemiology of IFI is shifting, with Pneumocystis, Mucorales, and Fusarium becoming more prevalent. While corticosteroids and GVHD remain key risk factors, factors such as chronic lung disease are increasing its importance. Prolonged neutropenia may have decreased in relevance, likely due to prophylaxis. Preventing PJP has become a new challenge in IFI management.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144732257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brown locusts, Locustana pardalina, host fluconazole resistant Candidozyma (Candida) auris, closely related to Clade III clinical strains.","authors":"Adepemi Ogundeji, Maryam Bello-Akinosho, Vaughn Swart, Jonathan Featherston, Errol D Cason, Armand Bolsenbroek, Carel Beneke, Jolly Musoke, Tyla Baker, Arshad Ismail, Olihile Sebolai, Jacobus Albertyn, Carolina Pohl","doi":"10.1093/mmy/myaf069","DOIUrl":"https://doi.org/10.1093/mmy/myaf069","url":null,"abstract":"<p><p>The environmental niche and mode of transmission from the environment to humans of the emerging pathogenic yeast, Candidozyma (Candida) auris is a subject of speculation, with hypotheses including avian species and marine environments. Interestingly, yeasts related to C. auris have been repeatedly observed associated with various insects. This prompted us to investigate a thermophilic insect, Locustana pardalina as possible host for C. auris. Here we report the isolation and identification of three C. auris strains from the gut of L. pardalina as well as the phenotypic characterisation of one of these isolates. Interestingly, the isolate was able to survive at 50°C and grew at 15% NaCl. In addition, it was susceptible to the tested disinfectants and antifungals, except fluconazole. Genome sequencing and single-nucleotide polymorphism analyses placed the isolate in Clade III, which is common in South African hospitals. This highlights the potential role of thermotolerant insects in the evolution and dissemination of emerging pathogenic yeasts.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"Reactivity of Cryptococcal Lateral Flow Assay in Aspergillosis, Histoplasmosis, Paracoccidioidomycosis, Candidiasis, Trichosporonosis, Bacterial and Viral infections\".","authors":"Edite Hatsumi Yamashiro-Kanashiro, Kelly Aparecida Kanunfre, Evanthia Vetos Mimicos, Vera Lúcia Teixeira de Freitas, Mussya Cisotto Rocha, Érika Yoshie Shimoda Nakanishi, Márcia Eiko Miyachi, Marjorie Vieira Batista, Roberto Martinez, Marcelo Nobrega Litvoc, Nairo Massakazu Sumita, Cláudia de Abreu Fonseca, Hélio Gomes Rodrigues, Eduardo Ronner Lagonegro, Maria Aparecida Shikanai Yasuda","doi":"10.1093/mmy/myaf068","DOIUrl":"https://doi.org/10.1093/mmy/myaf068","url":null,"abstract":"<p><p>Considering the need for a rapid, sensitive, and specific test for the early diagnosis of cryptococcal meningitis in critical regions where lumbar puncture and culture are inaccessible, we analyzed the specificity of the Lateral Flow Assay for cryptococcal antigen (LFA) in 217 serum specimens. Group 1: 68 HIV-uninfected patients with paracoccidioidomycosis, histoplasmosis, aspergillosis, trichosporonosis and 21 with tuberculosis; Group 2: 149 patients with HIV infection, including seven with histoplasmosis, and one with aspergillosis, and Group 3 with 24 proven cryptococcosis patients. Cross-reactivity of cryptococcal mannans and polysaccharides secreted by Paracoccidioides brasiliensis, Histoplasma capsulatum, and Trichosporon spp has been described in vitro. However, only a few cases of positive LFA tests in aspergillosis, trichosporonosis, candidemia, and bacterial infections sera have been reported. We observed false-positive LFA in 2/29 aspergillosis specimens but not in other mycoses or tuberculosis. Among 149 HIV-infected patients, three specimens tested positive, two had cytomegalovirus infections, one of whom also had toxoplasmosis and the other, Kaposi's sarcoma; one patient had no opportunistic infections. We observed sensitivities of 0.933 (serum), 0.95 (CSF), and 1.0 (serum or CSF) for LFA, and for all negative controls (N = 217, serum), a specificity of 0.977 and a negative predictive value (NPV) of 0 938. The specificity and NPV were 0.964 and 0 791, respectively, for 55 patients with mycoses; and 0.98 and 0.912 for 149 HIV-infected patients. We confirmed LFA's high specificity and accuracy for the control groups. There were 6.89% of false-positive results for aspergillosis, and no false-positive results for paracoccidioidomycosis, histoplasmosis, tuberculosis, or other bacterial diseases.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of in-house ELISA for Scedosporium/Lomentospora serodiagnosis.","authors":"Coralie Barrera, Marie-Elisabeth Bougnoux, Claire Hoffmann, Céline Damiani, Damien Costa, Florent Morio, Judith Fillaux, Sandrine Houze, Sophie Brun, Florence Persat, Jordan Leroy, Arnaud Fekkar, Sébastien Imbert, Jean-Philippe Bouchara, Taieb Chouaki, Eric Dannaoui, Laurence Millon, Anne-Pauline Bellanger","doi":"10.1093/mmy/myaf067","DOIUrl":"https://doi.org/10.1093/mmy/myaf067","url":null,"abstract":"<p><p>Scedosporium/Lomentospora species are ubiquitous molds that can cause deep-seated infections and allergic bronchopulmonary mycoses (ABPM). Serodiagnosis is currently performed by immunoprecipitation techniques (IP), which are time-consuming and lack reproducibility. In addition, as antigenic extracts for these fungi are not commercially available, many centers stopped performing this analysis. Therefore, there is a need for automated quantitative alternatives, such as ELISA. The aim of this study was to develop an ELISA for serodiagnosis of Scedosporium/Lomentospora infections. All sera received for Scedosporium/Lomentospora serodiagnosis expertise from April 2022 to February 2024 were tested in parallel using IP and an in-house ELISA with antigenic extracts from both S. apiospermum and L. prolificans. Clinical and biological data such as positive culture, total IgE level and final diagnosis retained were also collected prospectively. The concordance between techniques was calculated, with chi² tests performed to investigate the correlation between ELISA and culture results or final diagnosis. We tested 58 serum samples from 41 different patients. The concordance between IP and ELISA was 64% for S. apiospermum and 62% for L. prolificans. ELISA results obtained with S. apiospermum antigen extract were significantly correlated with culture results (p < 0.001, chi² test). ELISA was also more effective than IP to diagnose ABPM. The Scedosporium/Lomentospora ELISA gave satisfactory results, particularly for S. apiospermum. Further validation on a larger cohort is required implementing this ELISA for routine practice instead of IP. In addition, studies should be conducted on purified native proteins or combination with recombinant antigens to improve standardization.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High prevalence of Enterocytozoon bieneusi (microsporidia) in asymptomatic schoolchildren, Zambia.","authors":"Mable Mutengo, Alejandro Dashti, Michaela Liptáková, Namwiinga R Mulunda, Freeman W Chabala, Kyoko Hayashida, Stanley Chinyanta, Kelly Chisanga, James Mwansa, Pamela C Köster, Mónica Santín, Javier Sotillo, Sergio Sánchez, David Carmena","doi":"10.1093/mmy/myaf065","DOIUrl":"https://doi.org/10.1093/mmy/myaf065","url":null,"abstract":"<p><p>Microsporidia are single-celled, fungi-related eukaryotic intracellular parasites able to infect a wide diversity of invertebrate and vertebrate hosts. Among them, Enterocytozoon bieneusi and Encephalitozoon spp. (including Enc. cuniculi, Enc. hellem, and Enc. intestinalis) are known causative agents of infectious diseases in immunocompromised individuals including HIV/AIDS patients and organ transplant recipients. Additionally, asymptomatic microsporidial infections seem more frequent than initially anticipated and might represent an overlooked public health threat. Here we provide novel data on the occurrence and genetic diversity of microsporidial infections in individual stool samples (n = 247) collected from apparently healthy schoolchildren (age range: 5-18 years; male/female ratio: 1.1) in Lusaka, Zambia. Stool DNA samples were analysed by PCR and Sanger sequencing methods. A basic epidemiological questionnaire was used to retrieve data on variables potentially linked with higher odds of harbouring E. bieneusi infections. A high prevalence rate was found for E. bieneusi (9.3%, 23/247; 95% CI: 6.0-13.6) whereas Enc. intestinalis was much less frequent (0.4%, 1/247; 95% CI: 0.01-2.2). Four known (D, S2, S6, and Type IV) and three novel (HhZbEb1, HhZbEb2, and HhZbEb3) genotypes were identified within E. bieneusi. Genotype D was the predominant genotype found (30.8%, 4/13), followed by genotypes Type IV, HhZbEb2, and HhZbEb3 (15.4%, 2/13, each), and genotypes S2, S6, and HhZbEb1 (7.7%, 1/13, each). The only Encephalitozoon-positive sample was identified as Enc. intestinalis. Subclinical infections by E. bieneusi were common in the investigated paediatric population. Infected children could act as disregarded spreaders of microsporidial pathogens at the community level, thus representing a potential public health concern.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Octyl gallate as a treatment for Candida albicans-induced vaginal infection: Effects on fungal burden, inflammation, and Lactobacillus recovery in mice.","authors":"Yu-Tang Tung, Yi-Shan Kuo, Ya-Zhu Liu, Jin-Wei Xu, Shiming Li, Jyh-Horng Wu","doi":"10.1093/mmy/myaf066","DOIUrl":"https://doi.org/10.1093/mmy/myaf066","url":null,"abstract":"<p><p>Vulvovaginal candidiasis (VVC), primarily caused by Candida albicans, affects a large proportion of women and often recurs due to drug resistance. This study investigates octyl gallate, a naturally derived compound, as a novel treatment for VVC in a mouse model. Female BALB/c mice were infected with C. albicans and treated intravaginally with octyl gallate at low and high concentrations. Results demonstrated that octyl gallate significantly reduced fungal burden, restored beneficial Lactobacillus populations, and improved histological features of the vaginal tissue. Moreover, levels of pro-inflammatory cytokines including IL-17A, IL-22, IL-23, and IL-1β were markedly reduced, suggesting anti-inflammatory activity. No severe systemic side effects or hematological abnormalities were observed. These findings highlight the therapeutic potential of octyl gallate as a natural, dual-action agent for managing VVC through both antifungal and immunomodulatory effects. Further research is needed to evaluate its efficacy and safety in clinical settings.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A notable azole-nonsusceptible Candida orthopsilosis in the Candida parapsilosis complex isolated from onychomycosis in Hue City, Central Vietnam.","authors":"Thi Minh Chau Ngo, Dong Duong Ton That, Phuong Anh Ton Nu, Le Chi Cao, Giang Tran Thi, Thi Bich Thao Do, Thi Ngoc Thuy Ha, Minh Tiep Vo, Phuoc Vinh Nguyen, Ba Hoang Anh Mai, My Tra Nguyen Thi, Đac Hanh Nguyen, Thanh Huy Nguyen","doi":"10.1093/mmy/myaf064","DOIUrl":"https://doi.org/10.1093/mmy/myaf064","url":null,"abstract":"<p><p>The Candida parapsilosis complex, consisting of C. parapsilosis sensu stricto, C. orthopsilosis, and C. metapsilosis, is a major cause of Candida onychomycosis. Increasing reports of high levels of resistance to antifungal drugs, particularly fluconazole and echinocandin, have raised concerns about C. parapsilosis complex. This study investigates antifungal resistance and hydrolytic enzyme activity in these species. Species were identified using PCR-RFLP and ITS1-4 sequencing. Antifungal susceptibility was assessed using Sensititre™ YeastOne™. Hydrolytic enzyme production was assessed by agar plate culture. Among 43 isolates, C. parapsilosis sensu stricto was most prevalent (48.8%, n = 21/43), followed by C. orthopsilosis (39.6%, n = 17/43) and C. metapsilosis (11.6%, n = 5/43). All C. parapsilosis sensu stricto isolates were susceptible to antifungal agents, except 4.8% (n = 1/21) showing dose-dependent susceptibility to fluconazole and 4.8% (n = 1/21) resistance to amphotericin B. C. orthopsilosis showed significant resistance to fluconazole and voriconazole (52.9% each, n = 9/17), posaconazole (23.5%, n = 4/17), and low resistance to amphotericin B (5.9%, n = 1/17). One C. metapsilosis isolate (20%) showed cross-resistance to fluconazole and voriconazole, and another (20%) was resistant to 5-flucytosine. Enzymatic assays showed higher protease and lipase activity in C. parapsilosis sensu stricto and C. orthopsilosis compared to C. metapsilosis, with C. parapsilosis sensu stricto showing the highest protease activity. Comprehensive research into antifungal susceptibility and virulence factors of the C. parapsilosis species complex is essential to monitor the growing threat of antifungal resistance and to better understand its role in onychomycosis pathogenesis.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Statistical Analysis of Pulmonary Mycosis in a Tertiary Hospital in Eastern China Before and After the Pandemic of COVID-19.","authors":"Ge-Ge Cao, Ran Wang, Qing-Tian Hu, Yong Zhang, De-Sheng Wu","doi":"10.1093/mmy/myaf058","DOIUrl":"https://doi.org/10.1093/mmy/myaf058","url":null,"abstract":"<p><p>Following the complete relaxation of COVID-19 epidemic control measures in China by the end of 2022, the number of patients with pulmonary mycosis admitted to hospitals across the country has exhibited a more pronounced upward trend. However, statistical data is lacking to determine whether there is a significant correlation between COVID-19 and pulmonary mycosis. This study collected baseline information, the first laboratory indicators after admission, the types of pathogens, and the prognosis data of patients with pulmonary fungal infections from a tertiary hospital in northern Anhui Province from January 1, 2017, to December 31, 2023, to reveal any association between COVID-19 infection and pulmonary fungal infections. The research results indicated that the G and GM test levels of patients who recovered from COVID-19 and those currently experiencing active infection with COVID-19 were significantly higher than those of patients with pulmonary fungal infections who had never been infected with COVID-19. Infection with COVID-19 and other viruses (excluding COVID-19), mechanical ventilation, and concurrent solid tumors were identified as independent risk factors for poor prognosis in patients with pulmonary fungal diseases. Among patients with viral infections, COVID-19 infection was the most common, with 25 cases (41.67%), followed by herpes simplex virus infection, with 15 cases (25.00%).</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144675275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of potential targets in bone destruction by Talaromyces marneffei: Insights from data-independent acquisition proteomics.","authors":"Junhong Zhou, Deshuang Xi, Yilin Teng, Yanbin Feng, Baicheng Wan, Shaohui Zong, Gaofeng Zeng","doi":"10.1093/mmy/myaf063","DOIUrl":"10.1093/mmy/myaf063","url":null,"abstract":"<p><p>To investigate the molecular mechanisms of Talaromyces marneffei (TM)-induced bone destruction through proteomic analysis using Data-Independent Acquisition (DIA) technology. Bone tissue samples were collected from eight patients (four TM-infected cases, four non-infectious controls). Samples underwent histopathological evaluation (Hematoxylin and Eosin Staining and Wright-Giemsa staining), DIA proteomics analysis, and protein validation through immunohistochemistry and enzyme-linked immunosorbent assay (ELISA). Comparative analysis between Control (Con) and Infected (Inf) groups showed similar demographics but significantly elevated inflammatory markers in Inf. Histopathology revealed extensive bone destruction, marked inflammatory infiltration, fibrinoid necrosis, and altered hematopoietic cell populations in Inf specimens compared to Con. DIA proteomics identified 5930 quantifiable proteins, with 509 differentially expressed proteins (DEPs) between groups. Gene ontology and Kyoto encyclopedia of genes and genomes pathway analyses revealed significant enrichment of inflammation and immune response-related functions in Inf. COMMD1 was significantly downregulated while IL-17 was upregulated in Inf, as validated by immunohistochemistry and ELISA. DIA proteomics identified downregulated COMMD1 and upregulated IL-17 in TM-induced bone destruction, suggesting potential diagnostic biomarkers and therapeutic targets through inflammatory pathway modulation.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Time- and temperature-dependent Pentraxin 3 stability in serum and bronchoalveolar lavage fluid samples.","authors":"Radim Dobiáš, Valeria Skopelidou, Andrea Langer Sermeño, Jan Strakoš, Dominika Luptáková, Hana Tomášková, Milan Raška, Jozef Škarda, Denisa Bázsóová, Vladimír Havlíček","doi":"10.1093/mmy/myaf057","DOIUrl":"10.1093/mmy/myaf057","url":null,"abstract":"<p><p>Pentraxin 3 (Ptx3) is an acute-phase protein that specifically targets fungal galactosaminogalactan and has been proposed as a promising biomarker for invasive fungal infections. However, its stability in clinical samples over time has not yet been established. This study aimed to evaluate the stability of Ptx3 in serum and bronchoalveolar lavage fluid (BALF) samples during mid- and long-term storage. A total of 44 serum and 52 BALF samples were examined or re-examined for Ptx3 concentrations using enzyme immunoassay in pooled and individual sample formats. Samples were stored at -80°C, -20°C, and +37°C for periods ranging from 0 to 56 months. Statistical analyses included a paired two-sample Wilcoxon signed-rank test, analysis of variance, Bonferroni test, and linear regression analysis. Ptx3 remained highly stable in serum and BALF samples for up to 8 months at -20°C, with variations ranging from -1.8% to +2.8%. Long-term stability was observed at -80°C for 48 months, followed by a slow decline in Ptx3 levels. In contrast, storage at +37°C resulted in rapid degradation, with a 36.5%-60.7% increase or a 92.9%-97% decrease in Ptx3 levels in serum and BALF, respectively. These findings confirm that Ptx3 is a stable and reliable biomarker for invasive fungal infections when appropriate storage conditions are maintained.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12247167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}