Medical mycology最新文献

{"title":"Occupational non-invasive aspergillosis: a national multicenter retrospective study from the French National Network for Occupational and Environmental Disease Monitoring and Prevention (RNV3PE) 2001 - 2023.","authors":"Antoine Michel, Christophe Paris, Anaelle Gelineau, Réjane Vallade, Julien Staub, Karine Risso, Jean-Pierre Gangneux, Anastasia Saade","doi":"10.1093/mmy/myag042","DOIUrl":"https://doi.org/10.1093/mmy/myag042","url":null,"abstract":"<p><p>Aspergillosis-related diseases arise from exposure to Aspergillus fungi in diverse work environments but occupational data remain limited. We conducted a multicenter study including all adult cases of occupational non-invasive aspergillosis reported to the French National Network for the Monitoring and Prevention of Occupational and Environmental Diseases (RNV3PE) between 2001 and 2023. Cases were identified on the basis of ICD-10 codes for aspergillosis (group B44 and related) and/or documented occupational exposure to Aspergillus. We aimed to describe clinical features, estimate prevalence, and identify factors associated with occupational disease claims. Mandatory reports provided data on demographics, underlying factors, disease type, and occupational characteristics; multivariate logistic regression and cluster analysis were performed. Immuno-allergic forms predominated (74%) in the 84 adults included (67% men; median age: 49 years). The prevalence of occupational non-invasive aspergillosis was 0.15 per 100 000 inhabitants. Documented exposure involved A. fumigatus (15%) or Aspergillus spp. (66%). Causality was considered high in 45% of cases; workplace eviction was recommended in 64% of cases, and 36% were advised to file a claim. Strong causality alone was predictive of claim submission. Occupational non-invasive aspergillosis appears to be underdiagnosed, highlighting the need to improve the identification and prevention of workplace exposures.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of Terbinafine Resistance in Human Dermatophytosis in Uruguay.","authors":"Andrés Puime, Victoria Bórmida, Michel Rosas","doi":"10.1093/mmy/myag039","DOIUrl":"https://doi.org/10.1093/mmy/myag039","url":null,"abstract":"<p><p>Trichophyton rubrum and the Trichophyton mentagrophytes complex are the leading etiologic agents of human dermatophytosis. Terbinafine remains the first-line therapy for most dermatophyte infections; however, resistant strains have been increasingly reported worldwide, and the first cases of T. indotineae in Latin America have recently been reported. To update the limited regional information on Trichophyton spp. terbinafine resistance, we conducted a prospective laboratory study in Montevideo, Uruguay, from July 2023 to July 2024, using samples obtained through routine dermatological practice. Isolates were identified by morphological analysis and Matrix-Assisted Laser Desorption Ionisation-Time of Flight Mass Spectrometry (MALDI-TOF). Terbinafine-resistant (TerR) clinical isolates were screened with the terbinafine-containing agar medium (TCAM) method, and their minimal inhibitory concentrations (MICs) for terbinafine and itraconazole were determined using the European Committee for Antimicrobial Susceptibility Testing (EUCAST) broth microdilution method. The squalene epoxidase gene (SQLE) of TerR isolates was sequenced to detect resistance-associated mutations. Of 1 252 processed samples, 543 were positive by microscopic examination and 281 by culture. Dermatophytes were identified in 231 of 281 positive cultures. The predominant species were T. rubrum (74.9%) and the T. mentagrophytes complex (19.9%), together accounting for 94.8% of isolates. Terbinafine susceptibility screening was performed on 171 isolates; 4 (2.34%) were resistant, with MICs ≥ 1 mg/L. Sequenced SQLE revealing amino acid substitutions at positions 393 or 397 in three T. rubrum and one T. mentagrophytes complex isolates. This study identifies the terbinafine resistance-associated mutations in Uruguay and highlights the urgent need for regional surveillance of this emerging phenomenon.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147776151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Verweija noviomagensis gen. sp. nov.-A novel member of the Onygenales incertae sedis isolated from a dystrophic nail.","authors":"Marcelo Sandoval-Denis, Eelco F J Meijer, Hazal Kandemir, Jan Dijksterhuis, Bert Gerrits van den Ende, Ferry Hagen","doi":"10.1093/mmy/myag022","DOIUrl":"10.1093/mmy/myag022","url":null,"abstract":"<p><p>The order Onygenales includes keratinophilic fungi, such as dermatophytes, that can cause onychomycosis. Although dermatophytes are the primary cause of these infections, some non-dermatophytic, keratinophilic onygenalean fungi have been reported as causing nail infections. Other such fungi are frequently isolated as surface contaminants of nails sent for culture, but are not etiologic agents of onychomycosis. Here we introduce a novel onygenalean fungus isolated in the Netherlands from a nail that was suspected to have a fungal infection. As only a single sample was available, etiologic involvement of the fungus could not be assessed; also, since a direct microscopic examination result was not available, the infection status of the nail remains unclear. This fungus, which we describe here as Verweija noviomagensis, is described morphologically with a gymnothecium composed of loose, interwoven hyphae lacking appendages, eight-spored asci, and bright yellow ascospores. A multilocus phylogeny with eight markers classified it within the order Onygenales; however, it was not placed in any defined family within the order.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13042248/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147444451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A decade of ocular fungal infections due to Penicillium-like fungi in France (2012-2021).","authors":"Lorra Monpierre, Eric Dannaoui, Olivier Lortholary, Karine Boukris-Sitbon, Lilia Merabet, Taieb Chouaki, Marc Pihet, Laurence Millon, Laurence Delhaes, Julie Bonhomme, Estelle Cateau, Frederic Dalle, Emilie Cardot, Nicole Desbois-Nogard, Muriel Nicolas, Milene Sasso, Elise Recalt, Alexandre Alanio, Jean-Pierre Gangneux, Yaye-Fincoura Senghor, Loic Favennec, Valerie Letsher-Bru, Laure Kamus, Sophie Cassaing, Florent Morio, Guillaume Desoubeaux, Fanny Lanternier, Dea Garcia-Hermoso, Françoise Botterel","doi":"10.1093/mmy/myag025","DOIUrl":"10.1093/mmy/myag025","url":null,"abstract":"<p><p>Penicillium, Talaromyces, and Purpureocillium species (so called Penicillium-like) are increasingly recognised as opportunistic fungal pathogens capable of causing a wide range of infections, from superficial to invasive. Although ocular infections caused by these environmental fungi have been reported in the literature, they remain relatively underrecognised. We conducted a multicentric retrospective study of Penicillium-like ocular cases reported in the RESeau de Surveillance des Infections Fongique (RESSIF) database of the French National Reference Center for Invasive Mycoses and Antifungals between 2012 and 2021. Among 55 cases of Penicillium-like invasive infections reported to the RESSIF network in France, a total of 24 cases (44%) were identified, which predominantly presented as keratitis (n = 22, 92%). Predisposing factors were as follows: contact lens wear (n = 11, 46%), topical corticosteroid use (n = 9, 37.5%), ocular trauma (n = 5, 21%), pre-existing corneal disease (n = 4, 17%), and previous ocular surgery (n = 2, 8%). Purpureocillium lilacinum was the predominant pathogen (n = 19, 79%), followed by four Penicillium species and one Talaromyces species. Direct examination of ocular samples was positive in 13 cases (54%). Antifungal susceptibility testing (European Committee on Antimicrobial Susceptibility Testing) revealed that all species exhibited high minimal inhibitory concentrations to amphotericin B (> 1 mg/l). Most patients were treated with a combination of topical voriconazole and amphotericin B (n = 15, 62.5%), which was administered alongside oral voriconazole in nine cases (37.5%). Surgical intervention was required in six cases (25%) and included keratoplasty (n = 4) and enucleation (n = 2). This study provides a valuable overview of Penicillium-like fungal ocular infections and highlights the importance of systematically surveilling filamentous fungal keratitis.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective molecular characterization of Trichophyton isolates from animals in Spain.","authors":"Kaitlyn Parra, Gemma Castellá, F Javier Cabañes","doi":"10.1093/mmy/myag030","DOIUrl":"10.1093/mmy/myag030","url":null,"abstract":"<p><p>Some species of the genus Trichophyton are among the most important causes of dermatophytosis worldwide, one of the most common mycoses which affect both humans and animals. The phylogeny of dermatophytes has shifted greatly in the past two decades with the introduction of molecular techniques. This work is a retrospective genetic analysis of 91 Trichophyton isolates obtained mainly from animals attending the Veterinary Hospital of the Universitat Autònoma de Barcelona between 1984 and 2025. The isolates were identified using conventional mycological methods and subsequently confirmed by Sanger sequencing of the rDNA Internal Transcribed Spacer (ITS) region. The species were identified as Trichophyton mentagrophytes (61.5%), Trichophyton erinacei (16.5%), Trichophytoneuropaeum (7.7%), Trichophyton equinum (5.5%), Trichophyton japonicum (4.4%), Trichophyton benhamiae (2.2%), and Trichophyton verrucosum (2.2%). Additionally, T. mentagrophytes isolates were grouped into different genotypes. Our study showed that isolates recovered from chinchillas and rabbits were identified as T. mentagrophytes genotypes III* and XXIV whereas isolates from hedgehogs were identified as T. erinacei. In guinea pigs, T. europaeum was the most common species. Most isolates recovered from dogs belonged to T. mentagrophytes genotypes II* and III*, although T. japonicum and T. europaeum were also recovered. In horses, isolates were identified as T. equinum and T. mentagrophytes genotype III*. Cow isolates were identified as T. verrucosum whereas those from goat and sheep were identified as T. mentagrophytes genotype V. Trichophyton indotinae (T. mentagrophytes genotype VIII) was not found. This long-term dataset illustrates the stability and diversity of animal association Trichophyton species over the time.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147674971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diutina cutanea sp. nov.-phylogenomic-based analysis of a novel clinically relevant ascomycetous yeast.","authors":"Sander Boden, Annemarie Zandijk, Shawn R Lockhart, Tjomme van der Bruggen, Bert Gerrits van den Ende, Marizeth Groenewald, Ferry Hagen","doi":"10.1093/mmy/myag029","DOIUrl":"10.1093/mmy/myag029","url":null,"abstract":"<p><p>Ascomycetous yeasts of the genus Diutina are rarely encountered in clinical routine diagnostics, but known to harbor species with decreased susceptibility to azole antifungals. This study describes a clinical strain representing a novel member of the genus Diutina, which we named Diutina cutanea. The clinical strain, isolated from a human foot wound, was molecularly identified by ITS/D1D2 ribosomal DNA sequencing. Extensive phenotypic characterization was done by culturing, microscopy, sporulation assays, assimilation tests, and antifungal susceptibility testing. Nanopore sequencing was performed for de novo genome assembly and comparative genomics. ITS/D1D2 ribosomal DNA sequencing resulted in a low identity score compared to other Diutina species. Morphological characterization showed round to oval cells of 2-3 × 3-8 µm in size, multilateral budding, and formation of pseudohyphae were observed. Colonies were white, glossy, smooth, butyrous, and had an entire margin. Growth on CHROMagar Candida Plus allowed phenotypic distinction between D. cutanea and related clinically relevant species. Antifungal susceptibility showed an elevated fluconazole MIC of 4 µg/ml. Genome sequencing placed D. cutanea distantly but basal to the non-pathogenic relatives D. scorzettiae, D. ranongensis, and D. siamensis. ANI analysis resulted in a pairwise similarity of 0.83-0.86 between D. cutanea and its relatives. In conclusion, phylogenomic and ANI analyses showed D. cutanea to be a unique taxon within the genus Diutina. Diutina cutanea can be reliably phenotypically discerned from other clinically relevant Diutina species by culturing on CHROMagar Candida Plus.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13123516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147675043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revisiting onychomycosis: Etiological agents and antifungal susceptibility profile.","authors":"H S Lalchungnunga, Nidhi Singla, Mala Bhalla, Jagdish Chander","doi":"10.1093/mmy/myag024","DOIUrl":"10.1093/mmy/myag024","url":null,"abstract":"<p><p>Onychomycosis has a prevalence varying from 2% to 8% at present. The incidence is rising day-by-day worldwide. There are only a few studies that have looked into resistance pattern of onychomycosis isolates, and so the present study was planned to study the epidemiology and mycological profile, including antifungal susceptibility testing, for patients presenting with onychomycosis. The present prospective study (January 2019 to June 2020) was conducted on a total of 92 clinically suspected patients of onychomycosis as per standard mycological techniques. Antifungal susceptibility testing (AFST) was done for itraconazole, terbinafine, griseofulvin, and amphotericin B as per Clinical Laboratory Standard Institute guidelines. Sixty-five out of 92 (70.6%) nail samples were positive for fungal aetiology on KOH examination, while 50 samples grew fungi. Nineteen (38%) were dermatophytes (95% CI: 24.6%-51.4%), while 31 (62%) of the isolates were non-dermatophytes (22 non-dermatophyte moulds, NDM [95% CI: 30.3%-57.7%]; and 9 yeast [95% CI: 7.4%-28.6%]). Onychomycosis was more common in males (55.3%) and in the age group 41-50 years. Twenty-one patients had diabetes. Great toe [40 (61.5%)] as affected nail and distal and lateral onychomycosis was the most common presentation [47(72.3%)]. Among dermatophytes, MIC range was 0.125-2 μg/ml for terbinafine, while it was 0.25-4 μg/ml for itraconazole. The MIC50 and MIC90 values were low for amphotericin B, while very high for griseofulvin. Among Aspergillus sp. and yeast isolates, minimum inhibitory concentration (MIC) range was low for terbinafine and itraconazole as 0.03-0.25μg/ml, 0.06-8μg/ml, 0.03-0.25μg/ml and 0.03-0.25 μg/ml respectively. Over the years, the treatment of onychomycosis has been shifted from griseofulvin to terbinafine and itraconazole as has been proven by the study too that MIC values for griseofulvin were very high. It is important to study and generate data regarding the prevalence and antifungal susceptibility profile of not only dermatophytes but also NDMs and yeast, which are increasingly isolated, for not only epidemiological purposes but also to plan targeted treatment at optimum doses of antifungals.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147486618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct virulence and azole resistance profiles of Candida albicans clinical isolates from patients with acute and recurrent vulvovaginal candidiasis.","authors":"Sofía C Angiolini, Emilse Rodríguez, Clarisa Manzone-Rodriguez, Paula A Icely, María Soledad Miró, Claudio D Abiega, Fernando O Riera, Pablo Iribarren, Juan Pablo Caeiro, Claudia E Sotomayor","doi":"10.1093/mmy/myag028","DOIUrl":"10.1093/mmy/myag028","url":null,"abstract":"<p><p>Candida albicans is the primary etiological agent of acute vulvovaginal candidiasis (AVVC) and its recurrent form (RVVC), conditions that differ in clinical presentation, inflammatory context, and response to antifungal therapy. Although virulence factors and antifungal resistance have been individually linked to disease severity, their integrated contribution to distinct clinical outcomes remains poorly defined. Here, we performed a comprehensive phenotypic characterization of 58 clinical C. albicans isolates recovered from women with AVVC (n = 25) or RVVC (n = 33), and C. albicans SC5314 was included for comparison. We assessed fungal dimorphism, cell surface hydrophobicity, biofilm-forming capacity (BFC), and the production of secreted aspartyl proteinases (SAP) and lipases (LIP). Antifungal susceptibility to azoles and other clinically relevant agents was determined using an automated system. Multivariate unsupervised analyses were applied to identify integrated virulence phenotypes. All clinical isolates exhibited efficient filamentation. AVVC isolates displayed enhanced adherence, biofilm metabolic activity, and LIP production, whereas RVVC isolates exhibited increased SAP activity and reduced BFC. Unsupervised clustering and UMAP analysis identified four virulence clusters, including profiles specifically associated with AVVC or RVVC, as well as mixed phenotypes. Notably, a high prevalence of azole resistance was observed in both groups, with resistance patterns aligning with specific virulence clusters. Collectively, these findings indicate that AVVC and RVVC are associated with distinct, niche-adapted C. albicans virulence programs that integrate enzymatic activity, biofilm traits, and antifungal resistance. This structured phenotypic heterogeneity provides a framework to reinterpret vulvovaginal candidiasis as a multifactorial and adaptive process with important implications for disease persistence and therapeutic failure.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147645692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canine coccidioidomycosis in Argentina: Epidemiological trends and evaluation of a rapid diagnostic assay.","authors":"Mariana Noelia Viale, Patricia Evangelina Mansilla, Diego H Cáceres, Flavia Gisele Vivot, María Cecilia López-Joffre, Jorge Alberto Iglesias Casal, Paola Alejandra Soldá, Adriana Inés Toranzo, Cristina Elena Canteros","doi":"10.1093/mmy/myag027","DOIUrl":"10.1093/mmy/myag027","url":null,"abstract":"<p><p>Coccidioidomycosis is an endemic fungal infection in northwestern Argentina, where domestic dogs serve as epidemiological sentinels. This study updates the epidemiology of canine coccidioidomycosis from 2015 to 2024 and assesses the performance of a lateral flow assay (LFA) for antibody detection. During this period, 1 734 serum specimens were evaluated by agar gel immunodiffusion (ID) at the hospital in the main endemic region, of which 506 (29%) tested positive, representing an increase compared with the previous decade. This rise reflects strengthened surveillance, improved access to reagents through the National Mycology Network, and heightened veterinary awareness. In 2 years of active surveillance (2019-2021), we obtained clinical and epidemiological information from 72 seropositive dogs, showing that musculoskeletal signs, particularly limb pain and lameness, were the most frequent presentations, consistent with disseminated disease. More than 60% of dogs were primarily indoor animals, suggesting local acquisition of infection and emphasizing the potential risk of human exposure. To compare serological methods, 225 sera sent for diagnosis by ID were tested by LFA and counterimmunoelectrophoresis. The LFA demonstrated very good agreement with both reference methods and provided the shortest turnaround time, supporting its utility as a rapid screening tool in veterinary practice. However, limitations include the lack of specimens from dogs with other mycoses and the inability to evaluate test performance in acute disease, where sensitivity may be reduced. Overall, these findings highlight the importance of canine surveillance, clinician awareness, and accessible diagnostic tools to improve the early recognition and management of coccidioidomycosis in endemic regions.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147574753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the vaginal microbiome and its metabolic potential in Colombian patients with recurrent vulvovaginal candidiasis.","authors":"Jeiser Marcelo Consuegra-Asprilla, Yesid Cuesta-Astroz, Ángel González","doi":"10.1093/mmy/myag026","DOIUrl":"10.1093/mmy/myag026","url":null,"abstract":"<p><p>Recurrent vulvovaginal candidiasis (RVVC) is a multifactorial condition in which vaginal microbiota dysbiosis plays a key role. This study aimed to characterize the vaginal microbiome of patients with RVVC using metagenomic sequencing. Vaginal scraping samples were collected from 34 women aged 20-47 years and classified into three groups: (1) 14 women with RVVC who had experienced 3-7 episodes of VVC in the previous year; (2) 9 women with severe RVVC, defined as ≥8 episodes in the last year; and (3) 11 healthy women as controls. The results revealed an increased relative abundance of bacteria associated with bacterial vaginosis-including Gardnerella vaginalis, G. swidsinskii, and Prevotella bivia-as well as higher levels of Lactobacillus iners in both RVVC groups. In contrast, healthy women showed a greater abundance of L. crispatus and L. gasseri. Diversity analyses indicated lower α-diversity in the healthy group compared to RVVC patients. Metabolic potential profiling showed a differential increase in sequences related to the phosphotransferase system, fructose/mannose metabolism, pentose phosphate pathway, and cysteine/methionine and purine metabolism in RVVC groups relative to controls; no significant differences were observed between RVVC groups, indicating that microbial profiles alone do not correlate with the degree of disease severity. These findings provide relevant insights into the taxonomic and functional characteristics of the vaginal microbiome in women with RVVC and may support the development of targeted therapeutic strategies.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13061635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147513329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}