Molecular Epidemiology and Antifungal Susceptibility of Sporothrix Isolates from Shanxi, China: A Retrospective Study and National Comparison.

IF 2.3 3区 医学 Q3 INFECTIOUS DISEASES
Ziping Song, Yujia Zhai, Long Zhou, Xiaorui Su, Xiao He, Ting Li, Kai Xu, Ruijun Zhang, Yuying Kang
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引用次数: 0

Abstract

This study characterizes the molecular epidemiology of Sporothrix species in Shanxi Province, a low-prevalence region in China, providing real data on genetic profiles and antifungal susceptibility patterns. Clinical records of sporotrichosis cases from a tertiary hospital in Shanxi (2019-2024) were retrospectively analyzed. Isolates were identified through multilocus sequencing targeting the internal transcribed spacer (ITS), calmodulin (CAL), β-tubulin (TUB), and mating-type locus (MAT) genes, with phylogenetic analysis. Antifungal susceptibility testing against seven agents was performed for both yeast and mycelial phases. Additionally, a review of Chinese case reports (2015-2025) was conducted to collect and summarize data on Sporothrix species identification across the country. All 11 isolates were confirmed as Sporothrix globosa, displaying high genetic similarity to the strains reported in most regions of China in previous studies. Terbinafine, ketoconazole, and itraconazole demonstrated good activity. Yeast-phase susceptibility was significantly higher for amphotericin B, itraconazole, and voriconazole (P<0.05, compared to the mycelial phase). Geospatial analysis indicated S. globosa dominance in reported areas, with persistent surveillance gaps in western China. This study from Shanxi provides a comprehensive overview of S. globosa as the predominant etiological agent in the region, consistent with the distribution pattern observed throughout China. Terbinafine demonstrated the highest in vitro activity against S. globosa.

山西产孢子丝菌分离株的分子流行病学和抗真菌敏感性:回顾性研究和全国比较。
本研究对中国孢子丝菌低流行区山西省孢子丝菌的分子流行病学特征进行了研究,为孢子丝菌遗传谱和抗真菌药敏模式提供了真实数据。回顾性分析山西省某三级医院2019-2024年孢子虫病病例的临床记录。通过内部转录间隔基因(ITS)、钙调蛋白(CAL)、β-微管蛋白(TUB)和交配型位点(MAT)基因的多位点测序鉴定分离株,并进行系统发育分析。对酵母菌和菌丝体进行了7种药物的药敏试验。此外,通过对2015-2025年中国病例报告的梳理,收集和汇总全国孢子丝菌种类鉴定数据。11株分离株均为全球孢子丝菌,与中国大部分地区报道的菌株具有较高的遗传相似性。特比萘芬、酮康唑和伊曲康唑表现出良好的活性。酵母期对两性霉素B、伊曲康唑和伏立康唑的敏感性显著高于对照组(P
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来源期刊
Medical mycology
Medical mycology 医学-兽医学
CiteScore
5.70
自引率
3.40%
发文量
632
审稿时长
12 months
期刊介绍: Medical Mycology is a peer-reviewed international journal that focuses on original and innovative basic and applied studies, as well as learned reviews on all aspects of medical, veterinary and environmental mycology as related to disease. The objective is to present the highest quality scientific reports from throughout the world on divergent topics. These topics include the phylogeny of fungal pathogens, epidemiology and public health mycology themes, new approaches in the diagnosis and treatment of mycoses including clinical trials and guidelines, pharmacology and antifungal susceptibilities, changes in taxonomy, description of new or unusual fungi associated with human or animal disease, immunology of fungal infections, vaccinology for prevention of fungal infections, pathogenesis and virulence, and the molecular biology of pathogenic fungi in vitro and in vivo, including genomics, transcriptomics, metabolomics, and proteomics. Case reports are no longer accepted. In addition, studies of natural products showing inhibitory activity against pathogenic fungi are not accepted without chemical characterization and identification of the compounds responsible for the inhibitory activity.
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