Aspergillus latus: A cryptic causative agent of aspergillosis emerging in Japan.

IF 2.7 3区医学 Q3 INFECTIOUS DISEASES

Medical mycology Pub Date : 2025-06-05 DOI:10.1093/mmy/myaf052

Saho Shibata, Momotaka Uchida, Sayaka Ban, Katsuhiko Kamei, Akira Watanabe, Takashi Yaguchi, Vit Hubka, Hiroki Takahashi

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引用次数: 0

Abstract

Allodiploid hybrid species, Aspergillus latus, belonging to section Nidulantes, is a hybrid of A. spinulosporus and an unknown species closely related to A. quadrilineatus and A. sublatus. This hybrid has often been misidentified as the species in section Nidulantes, such as A. nidulans, A. spinulosporus, A. sublatus, or other cryptic species. Aspergillus latus has not been reported in Japan as well as Asia so far. In this study, we screened 23 clinical strains identified as A. spinulosporus isolated in Japan from 2012 to 2023 and found seven A. latus strains. To characterize the A. latus strains, we conducted comprehensive phenotyping including morphological observation, whole genome sequences, and phylogenetic analysis based on calmodulin (CaM) gene. In addition, we conducted antifungal susceptibility testing for A. latus strains. As a result, the morphological characters of A. latus were more similar to those of A. spinulosporus compared to A. sublatus. However, the ascospore of A. latus differed from that of A. spinulosporus. Phylogenetic analysis revealed that different CaM alleles from the same isolate clustered separately with A. spinulosporus and A. sublatus, consistent with its hybrid origin. Furthermore, A. latus strains showed reduced susceptibility to caspofungin and amphotericin B compared to A. spinulosporus, while they were susceptible to azoles. Our results suggest that A. latus has been a causative pathogen of aspergillosis in Japan since 2013.

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曲霉：日本出现的一种曲霉病的隐性病原体。

异源二倍体杂交种lataspergillus属Nidulantes科，是spinulosporus与未知种quadrilineatus和sublatus的杂交品种。这种杂交种经常被误认为是nidulans、A. spinulosporus、A. subblatus或其他隐种。到目前为止，在日本和亚洲都没有发现这种病的报道。本研究对2012 - 2023年在日本分离的23株临床鉴定为spinulosporus的菌株进行筛选，筛选出7株latus菌株。为了对该菌株进行表征，我们基于钙调蛋白（calmodulin, CaM）基因进行了形态学观察、全基因组测序和系统发育分析等综合表型分析。此外，我们还对该菌株进行了抗真菌药敏试验。结果表明，与亚棘棘相比，长棘棘的形态特征与棘棘更为相似。但其子囊孢子与棘孢子囊孢子不同。在系统发育上与棘棘棘和亚棘棘聚为一群。此外，与spinulosporus相比，A. latus菌株对caspofungin和两性霉素B的敏感性降低，而对唑类药物敏感。我们的研究结果表明，自2013年以来，拉曲霉一直是日本曲霉病的致病病原体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medical mycology 医学-兽医学

CiteScore

5.70

自引率

3.40%

发文量

632

审稿时长

12 months

期刊介绍： Medical Mycology is a peer-reviewed international journal that focuses on original and innovative basic and applied studies, as well as learned reviews on all aspects of medical, veterinary and environmental mycology as related to disease. The objective is to present the highest quality scientific reports from throughout the world on divergent topics. These topics include the phylogeny of fungal pathogens, epidemiology and public health mycology themes, new approaches in the diagnosis and treatment of mycoses including clinical trials and guidelines, pharmacology and antifungal susceptibilities, changes in taxonomy, description of new or unusual fungi associated with human or animal disease, immunology of fungal infections, vaccinology for prevention of fungal infections, pathogenesis and virulence, and the molecular biology of pathogenic fungi in vitro and in vivo, including genomics, transcriptomics, metabolomics, and proteomics. Case reports are no longer accepted. In addition, studies of natural products showing inhibitory activity against pathogenic fungi are not accepted without chemical characterization and identification of the compounds responsible for the inhibitory activity.