Medical mycology最新文献_第4页

Comparison of broth microdilution and Etest® methods for susceptibility testing of amphotericin B in Candida auris. 肉汤微量稀释法与est®法对耳念珠菌两性霉素B药敏试验的比较。

IF 2.7 3区医学

Medical mycology Pub Date : 2025-02-28 DOI: 10.1093/mmy/myaf019

Domenica G De Luca, Xiao Rui Li, David C Alexander, Tanis C Dingle, Philippe J Dufresne, Linda M Hoang, Julianne V Kus, Caroline Sheitoyan-Pesant, Amrita Bharat

引用次数: 0

COVID-19-associated pulmonary aspergillosis in a tertiary care center in the Midwestern Region of Brazil. 巴西中西部地区三级医疗中心的COVID-19相关肺曲霉病

IF 2.7 3区医学

Medical mycology Pub Date : 2025-02-28 DOI: 10.1093/mmy/myaf021

Marina Miranda Ely, Andyane Freitas Tetila, Anamaria Mello Miranda Paniago, Luana Rossato

{"title":"COVID-19-associated pulmonary aspergillosis in a tertiary care center in the Midwestern Region of Brazil.","authors":"Marina Miranda Ely, Andyane Freitas Tetila, Anamaria Mello Miranda Paniago, Luana Rossato","doi":"10.1093/mmy/myaf021","DOIUrl":"10.1093/mmy/myaf021","url":null,"abstract":"Coronavirus disease 2019 (COVID-19)-associated pulmonary aspergillosis (CAPA) has emerged as a complication following COVID-19 infections. This study aimed to evaluate the clinical and epidemiological data of CAPA cases in a tertiary hospital. Conducted retrospectively from February 2020 to January 2024, it involved the analysis of medical records to identify CAPA cases based on the diagnostic criteria established by the European Confederation of Medical Mycology and the International Society for Human and Animal Mycology. Seven patients were identified as possible CAPA cases, of whom 71.43% had pre-existing comorbidities. All patients received corticosteroid therapy, and 42.86% required prolonged mechanical ventilation. The study revealed a high mortality rate, with 71.43% of patients succumbing to the condition. The diagnosis of CAPA is challenging due to the invasiveness of definitive tests, such as biopsies, and the lack of resources for essential diagnostics like galactomannan detection. Despite the limited sample size, the findings align with existing literature, indicating a high prevalence of comorbidities, prior corticosteroid use, and mechanical ventilation as significant risk factors. This study underscores the critical need for heightened awareness, early diagnosis, and proactive management strategies for CAPA in severely ill COVID-19 patients to reduce risks and improve patient outcomes.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143575786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human activity, not environmental factors, drives Scedosporium and Lomentospora distribution in Taiwan. 在台湾，人类活动而非环境因素，是影响斑孢菌和扁孢菌分布的主要因素。

IF 2.7 3区医学

Medical mycology Pub Date : 2025-02-28 DOI: 10.1093/mmy/myaf022

Hsin-Mao Wu, Yu-Hsuan Fan, Guan-Jie Phang, Wen-Ting Zeng, Khaled Abdrabo El-Sayid Abdrabo, Yu-Ting Wu, Pei-Lun Sun, Ying-Hong Lin, Yin-Tse Huang

{"title":"Human activity, not environmental factors, drives Scedosporium and Lomentospora distribution in Taiwan.","authors":"Hsin-Mao Wu, Yu-Hsuan Fan, Guan-Jie Phang, Wen-Ting Zeng, Khaled Abdrabo El-Sayid Abdrabo, Yu-Ting Wu, Pei-Lun Sun, Ying-Hong Lin, Yin-Tse Huang","doi":"10.1093/mmy/myaf022","DOIUrl":"10.1093/mmy/myaf022","url":null,"abstract":"Scedosporium and Lomentospora species are emerging fungal pathogens capable of causing severe infections in both immunocompetent and immunocompromised individuals. Previous environmental surveys have suggested potential associations between these fungi and various soil chemical parameters, though the relative influence of human activity versus environmental factors has not been systematically evaluated. Here, we conducted a comprehensive survey of 406 soil samples from 132 locations across Taiwan, analyzing fungal abundance alongside soil physicochemical parameters and the Human Footprint Index (HFI). We recovered 236 fungal isolates comprising 10 species, with S. boydii (32.2%), S. apiospermum (30.9%), and S. dehoogii (14.4%) being the most prevalent. The highest fungal burdens were observed in urban environments (up to 1293 CFU/g), particularly in public spaces and healthcare facilities. Statistical analysis revealed a significant positive correlation between fungal abundance and HFI (r = 0.143, P = .005), while soil chemical parameters including nitrogen, carbon, pH, electrical conductivity, and various base cations showed no significant associations despite their wide ranges. These findings indicate that anthropogenic disturbance of environments, rather than soil chemistry, is the primary driver of Scedosporium and Lomentospora distribution in Taiwan. This understanding holds important implications for predicting infection risks and developing targeted public health strategies, particularly in rapidly urbanizing regions. Future studies incorporating more specific indicators of human impact may further elucidate the mechanisms underlying these distribution patterns.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histoplasmosis screening using urinary antigen detection in people living with HIV in Southern Brazil. 在巴西南部使用尿抗原检测HIV感染者的组织胞浆菌病筛查。

IF 2.7 3区医学

Medical mycology Pub Date : 2025-02-28 DOI: 10.1093/mmy/myaf017

Bianca Dos Santos Blan, Vanice Rodrigues Poester, Rossana Patricia Basso, Karine Ortiz Sanchotene, Diego H Caceres, Ivy Bastos Ramis de Souza, Alessandro C Pasqualotto, Melissa Orzechowski Xavier

{"title":"Histoplasmosis screening using urinary antigen detection in people living with HIV in Southern Brazil.","authors":"Bianca Dos Santos Blan, Vanice Rodrigues Poester, Rossana Patricia Basso, Karine Ortiz Sanchotene, Diego H Caceres, Ivy Bastos Ramis de Souza, Alessandro C Pasqualotto, Melissa Orzechowski Xavier","doi":"10.1093/mmy/myaf017","DOIUrl":"10.1093/mmy/myaf017","url":null,"abstract":"Histoplasmosis is a life-threatening opportunistic infection in individuals with advanced HIV disease, particularly in endemic regions such as Latin America. Early diagnosis is crucial for reducing mortality but remains underdiagnosed due to non-specific clinical presentations and limited diagnostic access. This study evaluates the impact of systematic histoplasmosis screening over 18 months (March 2021-September 2022) using the Histoplasma urinary antigen detection test in people living with HIV (PLHIV) receiving outpatient care or hospitalized at a reference center in Southern Brazil. A retrospective analysis was conducted on PLHIV screened with the Clarus® Histoplasma GM enzyme immunoassay (HGM-EIA, IMMY, USA). Inclusion criteria comprised a recent HIV diagnosis (≤ 30 days), poor adherence to or abandonment of antiretroviral therapy (>90 days), CD4 + count < 200 cells/mm³, or clinical/radiological findings suggestive of histoplasmosis. Clinical, laboratory, and treatment data were assessed, along with 90-day outcomes. Among 287 PLHIV screened, Histoplasma antigen was detected in 9.1% (26/287), including 19.6% of hospitalized patients and 6.4% of outpatients. Overall mortality was 24%, with a higher rate among inpatients (27.3%). Fever, neurological impairment, and lung opacification were more frequent in hospitalized cases. Amphotericin B deoxycholate was more commonly administered to inpatients (63.6% vs. 25%). Mortality at 90 days was significantly higher in patients with a histoplasmosis case-fatality score ≥ 5 (66.7% vs. 33.3%, P = .016). Systematic screening enhances early detection of histoplasmosis, facilitating timely treatment and reducing hospitalizations and mortality.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel in vivo observations of luliconazole 5% nail solution for onychomycosis: An ultrastructural study. 5%吕立康唑甲液治疗甲真菌病的体内观察：一项超微结构研究。

IF 2.7 3区医学

Medical mycology Pub Date : 2025-02-28 DOI: 10.1093/mmy/myaf016

Yuko Ehara, Nanako Yamada, Takashi Horie, Ryota Furuichi, Yuichi Yoshida, Osamu Yamamoto

{"title":"Novel in vivo observations of luliconazole 5% nail solution for onychomycosis: An ultrastructural study.","authors":"Yuko Ehara, Nanako Yamada, Takashi Horie, Ryota Furuichi, Yuichi Yoshida, Osamu Yamamoto","doi":"10.1093/mmy/myaf016","DOIUrl":"10.1093/mmy/myaf016","url":null,"abstract":"Lesional nails of 10 patients with onychomycosis (tinea unguium) were investigated by transmission electron microscopy (TEM) to gain an insight into in vivo morphological changes of dermatophytes after application of a clinical dosage of topical luliconazole (LLCZ) 5% nail solution. In these cases, Trichophyton rubrum (T. rubrum) was identified in three cases and T. interdigitale was identified in four cases (three unidentified cases; we were unable to identify either the genus or species). Specimens from tinea unguium before and after topical LLCZ application (maximum of 14 days) were observed by TEM. Two types of morphological changes of hyphae were revealed. Intracytoplasmic degeneration without antecedent obvious changes in the cell wall (type 1 degeneration) and degeneration of the fungal cell wall preceding intracytoplasmic changes (type 2 degeneration) were observed. We also examined in vivo morphological changes of dermatophytes in tinea pedis treated with ketoconazole (KCZ) to compare the morphological changes in the cell wall, plasma membrane, and cytoplasm to those after the application of LLCZ. Intracytoplasmic degeneration (type 1 degeneration) was observed in tinea pedis scales treated with topical KCZ. We confirmed that topical LLCZ 5% nail solution had acting points on the plasma membrane, cell wall, and cytoplasm of dermatophyte hyphae and that various degrees of morphological changes in lesional nails of tinea unguium occurred during treatment with topical LLCZ 5% nail solution.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COVID-19-associated invasive fungal infections in intensive care unit patients during the first pandemic waves in Argentina: Results of a single center experience. 阿根廷第一波大流行期间重症监护病房患者中与covid -19相关的侵袭性真菌感染：单一中心经验的结果

IF 2.7 3区医学

Medical mycology Pub Date : 2025-02-28 DOI: 10.1093/mmy/myaf024

Gladys Beatriz Posse, Fernanda Mariel Barberis, María Fernanda Benedetti, Daniel Pezzola, Katherine Hermida Alava, Carolina Rodríguez Laboccetta, Agustín Videla Garrido, Víctor Fernández Briceño, Paula Capece, Alejandro Nusblat, María Luján Cuestas

{"title":"COVID-19-associated invasive fungal infections in intensive care unit patients during the first pandemic waves in Argentina: Results of a single center experience.","authors":"Gladys Beatriz Posse, Fernanda Mariel Barberis, María Fernanda Benedetti, Daniel Pezzola, Katherine Hermida Alava, Carolina Rodríguez Laboccetta, Agustín Videla Garrido, Víctor Fernández Briceño, Paula Capece, Alejandro Nusblat, María Luján Cuestas","doi":"10.1093/mmy/myaf024","DOIUrl":"10.1093/mmy/myaf024","url":null,"abstract":"Critically ill COVID-19 patients are at high risk for invasive fungal infections (IFIs). Data on IFI prevalence in severe COVID-19 patients in Latin America are scarce. This study aimed at analyzing the prevalence and outcomes of IFIs in COVID-19 patients from Argentina. For this purpose, a retrospective study was conducted on COVID-19 patients admitted to the intensive care unit of a hospital in Buenos Aires between 2020 and 2022, with mycological evidence of IFI. A total of 86 cases of IFIs were reported, including 50 cases of COVID-19-associated candidiasis (CAC), 29 of COVID-19-associated pulmonary aspergillosis (CAPA), 10 of COVID-19-associated histoplasmosis (CAH), two cases of cryptococcemia, and one case of invasive fusariosis. Mixed fungal infections were also detected: two cases of Pneumocystis jirovecii pneumonia with CAPA, two cases of CAC with CAPA, one case of cryptococcemia with CAPA, one case of CAPA with CAH, and one case of CAC with CAPA and CAH. The overall mortality was 67.4%, with mortality of 59.6%, 72.7%, and 62.5% for CAC, CAPA, and CAH, respectively. All cases with mixed fungal infections were fatal. The most frequent underlying comorbidities were arterial hypertension, type-2 diabetes mellitus, obesity, smoking, oncohematological disease, chronic kidney disease, and chronic obstructive pulmonary disease. Candida parapsilosis, C. albicans, and C. tropicalis were the most common species in CAC. Aspergillus fumigatus, A. flavus, A. terreus, and A. niger were predominant in CAPA. In conclusion, this study highlights the high prevalence and mortality of CAC, CAPA, and CAH in severe COVID-19 patients from Argentina.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143575789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Secreted factors of Aspergillus fumigatus cause lung epithelial barrier disruption: A study using an air-liquid interface cell culture model. 曲霉菌的分泌因子会导致肺上皮屏障破坏：利用气液界面细胞培养模型进行的研究

IF 2.7 3区医学

Medical mycology Pub Date : 2025-02-28 DOI: 10.1093/mmy/myaf018

Bianca Carla Silva Campitelli Barros, Debora Tereza Lucas Barros, Matthias Brock, Marcos Sergio Toledo, Solange Maria Toledo Serrano, Erika Suzuki, Amir M Ghaemmaghami

引用次数: 0

Multicentric prospective evaluation of Pneumocystis jirovecii fungal load in bronchoalveolar lavage fluid fractions using qPCR. 应用qPCR技术对支气管肺泡灌洗液中肺囊虫真菌负荷的多中心前瞻性评价。

IF 2.7 3区医学

Medical mycology Pub Date : 2025-02-28 DOI: 10.1093/mmy/myaf023

Théo Ghelfenstein-Ferreira, Lize Cuypers, Anaïs Pinto, Guillaume Desoubeaux, Vicente Friaza, Stefan Fuchs, Catriona Halliday, Solène Le Gal, Ulrike Scharmann, Joerg Steinmann, Anne Totet, Sean Zhang, Mario Cruciani, Rosemary Barnes, J Peter Donnelly, Juergen Loeffler, P Lewis White, Alexandre Alanio

{"title":"Multicentric prospective evaluation of Pneumocystis jirovecii fungal load in bronchoalveolar lavage fluid fractions using qPCR.","authors":"Théo Ghelfenstein-Ferreira, Lize Cuypers, Anaïs Pinto, Guillaume Desoubeaux, Vicente Friaza, Stefan Fuchs, Catriona Halliday, Solène Le Gal, Ulrike Scharmann, Joerg Steinmann, Anne Totet, Sean Zhang, Mario Cruciani, Rosemary Barnes, J Peter Donnelly, Juergen Loeffler, P Lewis White, Alexandre Alanio","doi":"10.1093/mmy/myaf023","DOIUrl":"10.1093/mmy/myaf023","url":null,"abstract":"Bronchoalveolar lavage fluid (BALF) is the key sample type for diagnosing Pneumocystis jirovecii pneumonia, with quantitative PCR (qPCR) providing high sensitivity and specificity. However, sample processing varies considerably between laboratories, and optimal nucleic acid extraction method for BALF remains undetermined. This retrospective multicenter study, conducted in 12 centers as part of the fungal PCR initiative, assessed the efficacy of P. jirovecii detection by qPCR in different BALF fractions, including whole (WHO), pellet (PEL), and supernatant (SUP). Samples that were P. jirovecii-qPCR-positive during routine testing were divided into the three predefined fractions prior to nucleic acid extraction and qPCR, comparing detection rates and quantification cycle (Cq) values. Out of 113 P. jirovecii-qPCR-positive BALF samples, 339 qPCR measurements were analyzed. The PEL fraction demonstrated a similar detection rate to the WHO fraction, with positivity rates of 92.9% and 88.5%, respectively. The SUP fraction showed a lower positivity rate of 71.7%, dropping to 47% for high Cq values (Cq > 35). Quantitative analysis showed that the SUP fraction consistently yielded higher Cq values, trailing by 3.05 cycles compared to WHO, while PEL showed a smaller deviation (0.49 cycles), confirming its efficiency in retaining P. jirovecii genetic material for qPCR detection. The study concludes that the SUP fraction is suboptimal for P. jirovecii detection due to higher Cq values, suggesting lower fungal loads. The PEL and WHO fractions are comparable, suggesting that the PEL is a viable alternative, permitting the concentration of larger BALF volumes to levels that can be extracted across a range of platforms.","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":" ","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143575797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paracoccidioidomycosis-related hospitalization and mortality rates in the state of São Paulo, Brazil: A study using socioeconomic, demographic, and climatic predictors. 巴西圣保罗州与副球孢子菌病相关的住院率和死亡率：一项使用社会经济、人口和气候预测指标的研究

IF 2.7 3区医学

Medical mycology Pub Date : 2025-01-25 DOI: 10.1093/mmy/myaf014

Isabela Spinelli, Leandro Roberto de Macedo, Gabriella Freitas Ferreira

引用次数: 0

The impact of the COVID-19 on the antifungal consumption and antifungal resistance in Candida species isolated from the blood cultures of critically ill patients in intensive care units. 2019冠状病毒病对重症监护病人血培养念珠菌抗真菌消耗和耐药性的影响

IF 2.7 3区医学

Medical mycology Pub Date : 2025-01-25 DOI: 10.1093/mmy/myaf002

Emre Kara, Gokhan Metan, Dolunay Gülmez, Sevtap Arikan-Akdagli

引用次数: 0