Value of qPCR in the screening strategy for Candida auris.

IF 2.7 3区 医学 Q3 INFECTIOUS DISEASES
Sarah Jolivet, Sandra Fournier, Jeanne Bigot, Lokman Galal, Edith Le Roux, Mehdi Bonnet, Valérie Souyri, Pierre-Antoine Michel, Hélène Goulet, Gilles Pialoux, Marine Nadal, Muriel Fartoukh, Frédéric Barbut, Christophe Hennequin, Juliette Guitard
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引用次数: 0

Abstract

Candida auris has emerged as a major fungal pathogen responsible for persistent outbreaks, highlighting the importance of an effective screening strategy to control its spread in healthcare settings. Following a confirmed C. auris infection, we incorporated a specific qPCR assay into the screening process. Contact patients and environmental samples were screened weekly using culture and qPCR methods. We used these results to manage patients' cohorting, room disinfection, and new admissions in real time. The qPCR allowed us to identify 37 colonized patients and detect 47 contaminated environmental sites. While being specific, qPCR was more sensitive than culture in detecting C. auris colonization, enabling prompt infection control measures. This strategy was effective as the spread of C. auris in our institution was contained within 2 months. Our results support the inclusion of specific qPCR in the screening strategy for the early detection of C. auris in healthcare facilities.

qPCR在耳念珠菌筛选中的应用价值。
耳念珠菌已成为造成持续暴发的主要真菌病原体,这突出了在卫生保健环境中采取有效筛查策略以控制其传播的重要性。在确诊的金黄色葡萄球菌感染后,我们将特定的qPCR检测纳入筛选过程。每周使用培养和qPCR方法筛选接触者和环境样本。我们使用这些结果来实时管理患者队列、房间消毒和新入院患者。qPCR鉴定出37例定植患者,并检测出47个受污染的环境位点。qPCR具有特异性,在检测金黄色葡萄球菌定殖方面比培养更敏感,能够及时采取感染控制措施。这一策略是有效的,因为auris在我们机构的传播在2个月内得到了控制。我们的研究结果支持将特异性qPCR纳入筛查策略,以便在医疗机构中早期发现金黄色葡萄球菌。
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来源期刊
Medical mycology
Medical mycology 医学-兽医学
CiteScore
5.70
自引率
3.40%
发文量
632
审稿时长
12 months
期刊介绍: Medical Mycology is a peer-reviewed international journal that focuses on original and innovative basic and applied studies, as well as learned reviews on all aspects of medical, veterinary and environmental mycology as related to disease. The objective is to present the highest quality scientific reports from throughout the world on divergent topics. These topics include the phylogeny of fungal pathogens, epidemiology and public health mycology themes, new approaches in the diagnosis and treatment of mycoses including clinical trials and guidelines, pharmacology and antifungal susceptibilities, changes in taxonomy, description of new or unusual fungi associated with human or animal disease, immunology of fungal infections, vaccinology for prevention of fungal infections, pathogenesis and virulence, and the molecular biology of pathogenic fungi in vitro and in vivo, including genomics, transcriptomics, metabolomics, and proteomics. Case reports are no longer accepted. In addition, studies of natural products showing inhibitory activity against pathogenic fungi are not accepted without chemical characterization and identification of the compounds responsible for the inhibitory activity.
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