{"title":"Value of qPCR in the screening strategy for Candida auris.","authors":"Sarah Jolivet, Sandra Fournier, Jeanne Bigot, Lokman Galal, Edith Le Roux, Mehdi Bonnet, Valérie Souyri, Pierre-Antoine Michel, Hélène Goulet, Gilles Pialoux, Marine Nadal, Muriel Fartoukh, Frédéric Barbut, Christophe Hennequin, Juliette Guitard","doi":"10.1093/mmy/myaf041","DOIUrl":null,"url":null,"abstract":"<p><p>Candida auris has emerged as a major fungal pathogen responsible for persistent outbreaks, highlighting the importance of an effective screening strategy to control its spread in healthcare settings. Following a confirmed C. auris infection, we incorporated a specific qPCR assay into the screening process. Contact patients and environmental samples were screened weekly using culture and qPCR methods. We used these results to manage patients' cohorting, room disinfection, and new admissions in real time. The qPCR allowed us to identify 37 colonized patients and detect 47 contaminated environmental sites. While being specific, qPCR was more sensitive than culture in detecting C. auris colonization, enabling prompt infection control measures. This strategy was effective as the spread of C. auris in our institution was contained within 2 months. Our results support the inclusion of specific qPCR in the screening strategy for the early detection of C. auris in healthcare facilities.</p>","PeriodicalId":18586,"journal":{"name":"Medical mycology","volume":"63 5","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medical mycology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/mmy/myaf041","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
Abstract
Candida auris has emerged as a major fungal pathogen responsible for persistent outbreaks, highlighting the importance of an effective screening strategy to control its spread in healthcare settings. Following a confirmed C. auris infection, we incorporated a specific qPCR assay into the screening process. Contact patients and environmental samples were screened weekly using culture and qPCR methods. We used these results to manage patients' cohorting, room disinfection, and new admissions in real time. The qPCR allowed us to identify 37 colonized patients and detect 47 contaminated environmental sites. While being specific, qPCR was more sensitive than culture in detecting C. auris colonization, enabling prompt infection control measures. This strategy was effective as the spread of C. auris in our institution was contained within 2 months. Our results support the inclusion of specific qPCR in the screening strategy for the early detection of C. auris in healthcare facilities.
期刊介绍:
Medical Mycology is a peer-reviewed international journal that focuses on original and innovative basic and applied studies, as well as learned reviews on all aspects of medical, veterinary and environmental mycology as related to disease. The objective is to present the highest quality scientific reports from throughout the world on divergent topics. These topics include the phylogeny of fungal pathogens, epidemiology and public health mycology themes, new approaches in the diagnosis and treatment of mycoses including clinical trials and guidelines, pharmacology and antifungal susceptibilities, changes in taxonomy, description of new or unusual fungi associated with human or animal disease, immunology of fungal infections, vaccinology for prevention of fungal infections, pathogenesis and virulence, and the molecular biology of pathogenic fungi in vitro and in vivo, including genomics, transcriptomics, metabolomics, and proteomics. Case reports are no longer accepted. In addition, studies of natural products showing inhibitory activity against pathogenic fungi are not accepted without chemical characterization and identification of the compounds responsible for the inhibitory activity.