Microbes and Infection最新文献_第4页

Polymicrobial consortia in the pathogenesis of biofilm vaginosis visualized by FISH. Historic review outlining the basic principles of the polymicrobial infection theory 通过 FISH 观察生物膜阴道病发病机制中的多微生物联合体。概述多微生物感染理论基本原理的历史性回顾。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105403

Alexander Swidsinski , Rudolf Amann , Alexander Guschin , Sonja Swidsinski , Vera Loening-Baucke , Werner Mendling , Jack D. Sobel , Ronald F. Lamont , Mario Vaneechoutte , Pedro Vieira Baptista , Catriona S. Bradshaw , Igor Yu Kogan , Аlevtina M. Savicheva , Oleg V. Mitrokhin , Nadezhda W. Swidsinski , Gennadiy T. Sukhikh , Tatjana V. Priputnevich , Inna A. Apolikhina , Yvonne Dörffel

{"title":"Polymicrobial consortia in the pathogenesis of biofilm vaginosis visualized by FISH. Historic review outlining the basic principles of the polymicrobial infection theory","authors":"Alexander Swidsinski , Rudolf Amann , Alexander Guschin , Sonja Swidsinski , Vera Loening-Baucke , Werner Mendling , Jack D. Sobel , Ronald F. Lamont , Mario Vaneechoutte , Pedro Vieira Baptista , Catriona S. Bradshaw , Igor Yu Kogan , Аlevtina M. Savicheva , Oleg V. Mitrokhin , Nadezhda W. Swidsinski , Gennadiy T. Sukhikh , Tatjana V. Priputnevich , Inna A. Apolikhina , Yvonne Dörffel","doi":"10.1016/j.micinf.2024.105403","DOIUrl":"10.1016/j.micinf.2024.105403","url":null,"abstract":"<div><div>The manuscript disputes the exclusive mono-infectious way of thinking, which presumes that for every infection only one pathogen is responsible and sufficient, when infectious vectors, close contact and reduced immunity meet. In situations involving heavily colonized anatomical sites such an approach often ends in insoluble contradictions. Upon critical reflection and evaluation of 20 years research on spatial organization of vaginal microbiota it is apparent, that in some situations, pathogens may act and operate in permanent, structurally organized consortia, whereas its individual components may be innocuous and innocent, failing to express any pathogenic effect. In these cases, consortia are the true pathogens responsible for many infectious conditions, which usually remain unrecognized as long as improperly diagnosed.</div><div>The structure of such consortia can be unraveled using ribosomal fluorescence in situ hybridization (FISH). FISH methodology, that not only offers an ex vivo opportunity to recognize bacterial species, but provides unique physical insight into their specific role in the pathogenesis of polymicrobial infections. Ribosomal FISH technique applied to both, women with bacterial vaginosis (BV) and their male partners, has added significantly to our understanding of the pathogenesis of this condition and contributed to appreciating the mechanisms of polymicrobial, community-based infection, potentially leading to therapeutic advances.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105403"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bordetella pertussis outer membrane vesicles impair neutrophil bactericidal activity 百日咳杆菌外膜囊泡损害中性粒细胞的杀菌活性。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105375

Jimena Alvarez Hayes , Bruno Blancá , Juan Pablo Gorgojo, Carlos Baroli, Mariela del Carmen Carrica, Maria Eugenia Rodriguez

{"title":"Bordetella pertussis outer membrane vesicles impair neutrophil bactericidal activity","authors":"Jimena Alvarez Hayes , Bruno Blancá , Juan Pablo Gorgojo, Carlos Baroli, Mariela del Carmen Carrica, Maria Eugenia Rodriguez","doi":"10.1016/j.micinf.2024.105375","DOIUrl":"10.1016/j.micinf.2024.105375","url":null,"abstract":"<div><div><span><span><span>Neutrophils constitute the primary defense against bacterial infections, yet certain pathogens express </span>virulence factors that enable them to subvert neutrophils-mediated killing. Outer </span>membrane vesicles (OMVs) have emerged as a secretory system through which bacteria deliver virulence factors to host cells. OMVs from </span><span><em>Bordetella pertussis</em></span><span>, the etiological agent of whooping cough, are loaded with most of bacterial virulence factors, including CyaA, which plays a key role in </span><em>B. pertussis</em><span> evasion of neutrophils bactericidal activity. In our study, we investigated the role of </span><em>B. pertussis</em> OMVs in bacterial interaction with neutrophils. We observed that interaction of OMVs with neutrophils led to a decrease in the expression of cell surface CR3 and FcγRs, an effect dependent on the CyaA toxin delivered by these vesicles. This decreased receptor expression led to reduced bacterial uptake by neutrophils, irrespective of the presence of opsonic antibodies. Moreover, CyaA delivered by OMVs hindered intracellular bactericidal trafficking, promoting bacterial intracellular survival. When both bacteria and OMVs were opsonized, competition between opsonized OMVs and <em>B. pertussis</em> for FcγRs on neutrophils led to a significant decrease in bacterial uptake. Overall, our findings suggest that <em>B. pertussis</em><span> OMVs promote bacterial survival to the encounter with neutrophils in both naïve and immunized individuals.</span></div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105375"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

C1q modulation of antibody-dependent enhancement of dengue virus infection in human myeloid cell lines is dependent on cell type and antibody specificity C1q 对抗体依赖性增强人类髓系细胞登革病毒感染的调节作用取决于细胞类型和抗体特异性。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105378

Alana B. Byrne , Florencia A. Bonnin , Eduardo L. López , Fernando P. Polack , Laura B. Talarico

{"title":"C1q modulation of antibody-dependent enhancement of dengue virus infection in human myeloid cell lines is dependent on cell type and antibody specificity","authors":"Alana B. Byrne , Florencia A. Bonnin , Eduardo L. López , Fernando P. Polack , Laura B. Talarico","doi":"10.1016/j.micinf.2024.105378","DOIUrl":"10.1016/j.micinf.2024.105378","url":null,"abstract":"<div><div><span><span>Antibody-dependent enhancement (ADE) of dengue virus (DENV) infection is one of the mechanisms contributing to increased severity during heterotypic, secondary infection. The complement protein </span>C1q has been shown to reduce the magnitude of ADE </span><em>in vitro</em>. Therefore, we investigated the mechanisms of C1q modulation of ADE, focusing on processes of viral entry.</div><div><span>Using a model of ADE of DENV-1 infection in human myeloid cell lines<span> in the presence of monoclonal antibodies, 4G2 and 2H2, we found that C1q produced nearly a 40-fold reduction of ADE of DENV-1 in K562 cells, but had no effect in U937 cells. In K562 cells, C1q reduced adsorption of DENV-1/4G2 and exerted a dual inhibitory effect on adsorption and </span></span>internalization of DENV-1/2H2. Distinct endocytic pathways in the presence of antibody corresponded to conditions where C1q produced a differential action. Also, C1q did not affect the intrinsic cell response mediated by FcγR in human myeloid cells.</div><div><span>The modulation of ADE of DENV-1 by C1q is dependent on the FcγR expressed on immune cells and the specificity of the antibody comprising the immune complex. Understanding protective and pathogenic mechanisms in the </span>humoral response to DENV infections is crucial for the successful design of antivirals and vaccines.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105378"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of tuberculosis-specific antigen stimulation on the diagnostic accuracy of interferon-γ inducible protein-10 in distinguishing active and latent tuberculosis infection: a meta-analysis 结核特异性抗原刺激对干扰素-γ诱导蛋白-10区分活动性和潜伏性结核感染诊断准确性的影响：荟萃分析。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105396

Muhammad Iqhrammullah , Rika Yusnaini , Shakira Amirah , Intan Chaharunia Mulya , Ghina Tsurayya , Muhammad Alif Naufal , Sukmawan Fajar Santosa , Harapan Harapan , Baidillah Zulkifli

{"title":"Effect of tuberculosis-specific antigen stimulation on the diagnostic accuracy of interferon-γ inducible protein-10 in distinguishing active and latent tuberculosis infection: a meta-analysis","authors":"Muhammad Iqhrammullah , Rika Yusnaini , Shakira Amirah , Intan Chaharunia Mulya , Ghina Tsurayya , Muhammad Alif Naufal , Sukmawan Fajar Santosa , Harapan Harapan , Baidillah Zulkifli","doi":"10.1016/j.micinf.2024.105396","DOIUrl":"10.1016/j.micinf.2024.105396","url":null,"abstract":"<div><h3>Background</h3><div>Identifying active tuberculosis (ATB) from latent tuberculosis infection (LTBI) persists as a challenge, and interferon-γ inducible protein-10 (IP-10) has been employed as the solution. To further improve its diagnostic performance, the sample can be stimulated with TB specific antigen (TBAg).</div></div><div><h3>Aim</h3><div>To perform meta-analysis on diagnostic accuracy of unstimulated and TBAg-stimulated IP-10 in differentiating ATB from LTBI.</div></div><div><h3>Methods</h3><div>Systematic search was performed on five major scientific databases as of 29 November 2023. Observational studies reporting diagnostic values of unstimulated or TBAg-stimulated IP-10 in identifying ATB from LTBI were included. Meta-analysis was carried out using two-level mixed-effect logistic regression model.</div></div><div><h3>Results</h3><div>Twenty-five studies recruiting 2301 patients (1137 ATB versus 1164 LTBI) were included in the quantitative analysis. The pooled sensitivity and specifity of IP-10 were 72% (95%CI: 0.59–0.82) and 78% (95%CI: 0.63–0.88), respectively. As for TBAg-stimulated IP-10, the sensitivity and specifity were 82% (95%CI: 0.76–0.87) and 85% (95%CI: 0.73–0.92), respectively. The senstivity was reduced signiticantly (<em>p</em> < 0.01) when the patients with human immunodeficiency virus infection were included, except after the TBAg stimulation.</div></div><div><h3>Conclusion</h3><div>Stimulating IP-10 with TBAg could improve the diagnostic accuracy in differentiating ATB from LTBI.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105396"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning-based characterization of the gut microbiome associated with the progression of primary biliary cholangitis to cirrhosis 基于机器学习的与原发性胆汁性胆管炎发展为肝硬化相关的肠道微生物组特征描述

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105368

Qi Wang , Xiaomeng Tang , Wenying Qiao , Lina Sun , Han Shi , Dexi Chen , Bin Xu , Yanmin Liu , Juan Zhao , Chunyang Huang , Ronghua Jin

{"title":"Machine learning-based characterization of the gut microbiome associated with the progression of primary biliary cholangitis to cirrhosis","authors":"Qi Wang , Xiaomeng Tang , Wenying Qiao , Lina Sun , Han Shi , Dexi Chen , Bin Xu , Yanmin Liu , Juan Zhao , Chunyang Huang , Ronghua Jin","doi":"10.1016/j.micinf.2024.105368","DOIUrl":"10.1016/j.micinf.2024.105368","url":null,"abstract":"<div><h3>Background</h3><div><span>Primary biliary cholangitis (PBC) is associated closely with the </span>gut microbiota<span><span>. This study aimed to explore the characteristics of the gut microbiota after the progress of PBC to </span>cirrhosis.</span></div></div><div><h3>Method</h3><div>This study focuses on utilizing the 16S rRNA<span> gene sequencing method to screen for differences in gut microbiota<span> in PBC patients who progress to cirrhosis. Then, we divided the data into training and verification sets and used seven different machine learning (ML) models to validate them respectively, calculating and comparing the accuracy, F1 score, precision, and recall, and screening the dominant intestinal flora affecting PBC cirrhosis.</span></span></div></div><div><h3>Result</h3><div><span>PBC cirrhosis patients showed decreased diversity and richness of gut microbiota. Additionally, there are alterations in the composition of gut microbiota in PBC cirrhosis patients. The abundance of </span><span><span>Faecalibacterium</span></span> and <em>Gemmiger</em> bacteria significantly decreases, while the abundance of <span><span>Veillonella</span></span> and <span><span>Streptococcus</span></span> significantly increases. Furthermore, machine learning methods identify <span><span>Streptococcus</span></span> and <em>Gemmiger</em> as the predominant gut microbiota in PBC patients with cirrhosis, serving as non-invasive biomarkers (AUC = 0.902).</div></div><div><h3>Conclusion</h3><div>Our study revealed that PBC cirrhosis patients gut microbiota composition and function have significantly changed. <em>Streptococcus</em> and <em>Gemmiger</em> may become a non-invasive biomarker for predicting the progression of PBC progress to cirrhosis.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105368"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141137069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Loss of cilia in chemosensory neurons inhibits pathogen avoidance in Caenorhabditis elegans 化感神经元中纤毛的缺失会抑制秀丽隐杆线虫的病原体规避能力

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105370

Ming Lei , Yanheng Tan , Jingyi Ke , Mengqi Wang , Zeyang He , Guangshuo Ou , Haijun Tu , Weihong Tan

{"title":"Loss of cilia in chemosensory neurons inhibits pathogen avoidance in Caenorhabditis elegans","authors":"Ming Lei , Yanheng Tan , Jingyi Ke , Mengqi Wang , Zeyang He , Guangshuo Ou , Haijun Tu , Weihong Tan","doi":"10.1016/j.micinf.2024.105370","DOIUrl":"10.1016/j.micinf.2024.105370","url":null,"abstract":"<div><div>Pathogen avoidance is a crucial and evolutionarily conserved behavior that enhances survival by preventing infection in diverse species, including <span><span>Caenorhabditis elegans</span></span> (<em>C. elegans</em>). This behavior relies on multiple chemosensory neurons equipped with cilia that are exposed to the external environment. However, the specific role of neuronal cilia in pathogen avoidance has not been completely elucidated. Herein, we discovered that <em>osm-3(p802)</em> mutants, which lack chemosensory neuronal cilia, exhibit slower avoidance of the pathogen <span><span>Pseudomonas aeruginosa</span></span><span> PA14, but not </span><em>Escherichia coli</em> OP50. This observation was consistent when <em>osm-3(p802)</em> mutants were exposed to <em>P. aeruginosa</em><span> PAO1. Following an encounter with PA14, the pumping, thrashing, and defecation behaviors of </span><em>osm-3</em> mutants were comparable to those of the wild-type. However, the <em>osm-3</em><span> mutants demonstrated reduced intestinal colonization of PA14, suggesting that they have stronger intestinal clearance ability. We conducted RNA-seq to identify genes responding to external stimuli that were differentially expressed owing to the loss of </span><em>osm-3</em><span> and PA14 infection. Using RNAi, we demonstrated that three of these genes were essential for normal pathogen avoidance. In conclusion, our findings demonstrate that the loss of chemosensory neuronal cilia reduces pathogen avoidance in </span><em>C. elegans</em> while delaying intestinal colonization.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105370"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pertussis toxin-dependent and -independent protection by Bordetella pertussis against influenza 百日咳杆菌对流感的百日咳毒素依赖性和非依赖性保护。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105404

Thomas Belcher , Loïc Coutte , Anne-Sophie Debrie, Valentin Sencio, François Trottein, Camille Locht , Stephane Cauchi

{"title":"Pertussis toxin-dependent and -independent protection by Bordetella pertussis against influenza","authors":"Thomas Belcher , Loïc Coutte , Anne-Sophie Debrie, Valentin Sencio, François Trottein, Camille Locht , Stephane Cauchi","doi":"10.1016/j.micinf.2024.105404","DOIUrl":"10.1016/j.micinf.2024.105404","url":null,"abstract":"<div><div>Bacterial-viral co-infections are frequent, but their reciprocal effects are not well understood. Here, we examined the effect <em>Bordetella pertussis</em> infection and the role of pertussis toxin (PT) on influenza A virus (IAV) infection and disease. In C57BL/6J mice, prior nasal administration of virulent <em>B. pertussis</em> BPSM and PT-deficient BPRA provided effective and sustained protection from IAV-induced mortality. However, BPSM or BPRA administered together with purified PT (BPRA + PT) had a stronger protective effect on weight loss compared to BPRA alone, reduced the viral load, and induced IL-17A in the lungs. In IL-17<sup>−/−</sup> mice, BPSM- and BPRA + PT-mediated protection against viral replication was abolished, while BPSM, BPRA and BPRA + PT provided similar levels of protection against IAV-induced mortality and weight loss. In conclusion, <em>B. pertussis</em> infection protects against influenza by two mechanisms: one reducing viral replication depending on PT and IL-17, and the other, independently of PT and IL-17, resulting in protection against influenza disease without reducing the viral load.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105404"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141917087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lung infection with classical Klebsiella pneumoniae strains establishes robust macrophage-dependent protection against heterologous reinfection 经典肺炎克雷伯氏菌菌株的肺部感染可建立对异源再感染的强大巨噬细胞依赖性保护。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105369

Joseph J. Mackel , Casey L.G. Mick , Emily Guo , David A. Rosen

{"title":"Lung infection with classical Klebsiella pneumoniae strains establishes robust macrophage-dependent protection against heterologous reinfection","authors":"Joseph J. Mackel , Casey L.G. Mick , Emily Guo , David A. Rosen","doi":"10.1016/j.micinf.2024.105369","DOIUrl":"10.1016/j.micinf.2024.105369","url":null,"abstract":"<div><div>At present, there is no approved vaccine for prevention of infection by the opportunistic bacterium <em>Klebsiella pneumoniae</em> (Kp); success in treating these infections is increasingly challenged by the spread of antibiotic resistance. Preclinical investigation of adaptive immunity elicited by lung infection with live classical Kp may reveal host mechanisms of protection against this pathogen. Here, we utilize multiple virulent classical Kp strains to demonstrate that following lung infection, surviving wild-type mice develop protective immunity against both homologous and heterologous (heterotypic) reinfection. For Kp strains with low capacity to disseminate from the lung, this immunity is B-cell-independent. We further demonstrate that this immune protection is also effective against subsequent challenge with hypervirulent Kp if the strains share the same capsule type. Systemic inoculation fails to elicit the same protective effect as lung inoculation, revealing a lung-specific immune effector function is responsible for this protection. We therefore utilized clodronate-loaded liposomes to substantially deplete both alveolar macrophages and lung interstitial macrophages, finding that simultaneous depletion of both subsets entirely ablates protection. These findings indicate that following initial lung infection with Kp, lung macrophages mediate protection against ensuing Kp challenge.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105369"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of macozinone in mice with genetically diverse susceptibility to Mycobacterium tuberculosis infection 麦考嗪酮对不同基因的结核分枝杆菌感染小鼠的疗效

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105376

Boris Nikonenko , Nadezhda Logunova , Anna Egorova , Marina Kapina , Natalia Sterzhanova , Irina Bocharova , Elena Kondratieva , Olga Riabova , Lyudmila Semyonova , Vadim Makarov , Dedicated to the 10th anniversary of the iM4TB Foundation

{"title":"Efficacy of macozinone in mice with genetically diverse susceptibility to Mycobacterium tuberculosis infection","authors":"Boris Nikonenko , Nadezhda Logunova , Anna Egorova , Marina Kapina , Natalia Sterzhanova , Irina Bocharova , Elena Kondratieva , Olga Riabova , Lyudmila Semyonova , Vadim Makarov , Dedicated to the 10th anniversary of the iM4TB Foundation","doi":"10.1016/j.micinf.2024.105376","DOIUrl":"10.1016/j.micinf.2024.105376","url":null,"abstract":"<div><div><span>Host heterogeneity in pulmonary tuberculosis<span> leads to varied responses to infection and drug treatment. The present portfolio of anti-TB drugs needs to be boosted with new drugs and drug regimens. Macozinone, a clinical-stage molecule targeting the essential enzyme, DprE1, represents an attractive option. Mice (I/St, B6, (AKRxI/St)F1, B6.I-100 and B6.I-139) genetically diverse susceptibility to </span></span><span><em>Mycobacterium tuberculosis</em></span> (<em>Mtb</em>) H37Rv infection were subjected to aerosol- or intravenous infection to determine the efficacy of macozinone (MCZ). They were treated with macozinone or reference drugs (isoniazid, rifampicin). Lung and spleen bacterial burdens were measured at four and eight weeks post-infection. Lung histology was evaluated at four weeks of treatment. Treatment with macozinone resulted in a statistically significant reduction in the bacterial load in the lungs and spleen as early as four weeks after treatment initiation in mice susceptible or resistant to <em>Mtb</em><span> infection. In the TB hypoxic granuloma<span> model, macozinone was more potent than rifampicin in reducing the CFU counts. However, histopathological analysis revealed significant lung changes in I/St mice after eight weeks of treatment initiation. Macozinone demonstrated efficacy to varying degrees across all mouse models of </span></span><em>Mtb</em> infection used. These results should facilitate its further development and potential introduction into clinical practice.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105376"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141296330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Staphylococcus pseudintermedius and Pseudomonas aeruginosa Lubbock Chronic Wound Biofilm (LCWB): a suitable dual-species model for in vitro studies 假中间葡萄球菌和铜绿假单胞菌卢伯克慢性伤口生物膜（LCWB）：体外研究的合适双菌种模型。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105384

Silvia Di Lodovico , Morena Petrini , Paola Di Fermo , Valeria De Pasquale , Luisa De Martino , Simonetta D'Ercole , Francesca Paola Nocera , Mara Di Giulio

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