Microbes and Infection最新文献_第4页

Revisiting the concept of giant viruses 重新审视巨型病毒的概念。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105467

Jônatas Santos Abrahão

引用次数: 0

Proteolytic activity of secreted proteases from pathogenic leptospires and effects on phagocytosis by murine macrophages 致病性钩端螺旋体分泌蛋白酶的蛋白水解活性及其对小鼠巨噬细胞吞噬的影响。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2025.105469

Thais A. Amamura , Daniella dos S. Courrol , Angela S. Barbosa , Ildefonso A. Silva-Junior , Tiago F. da Silva , Leonardo M. Midon , Mario C. Cruz , Marcos B. Heinemann , Rosa M. Chura-Chambi , Ligia Morganti , Lourdes Isaac

{"title":"Proteolytic activity of secreted proteases from pathogenic leptospires and effects on phagocytosis by murine macrophages","authors":"Thais A. Amamura , Daniella dos S. Courrol , Angela S. Barbosa , Ildefonso A. Silva-Junior , Tiago F. da Silva , Leonardo M. Midon , Mario C. Cruz , Marcos B. Heinemann , Rosa M. Chura-Chambi , Ligia Morganti , Lourdes Isaac","doi":"10.1016/j.micinf.2025.105469","DOIUrl":"10.1016/j.micinf.2025.105469","url":null,"abstract":"<div><div>Leptospirosis is a zoonosis caused by spirochete <em>Leptospira.</em> Pathogenic leptospires evade the Complement System, enabling their survival upon contact with normal human serum <em>in vitro</em>. In a previous study, we demonstrated that proteases secreted by pathogenic leptospires cleave several Complement proteins, including C3 and the opsonins C3b and iC3b. We hypothesize that these <em>Leptospira</em> proteases, such as thermolysin and leptolysin, may decrease the phagocytic activity of murine peritoneal macrophages. We observed decreased amounts of CR3 and CR4 using flow cytometry when these cells were treated with supernatant from the culture of pathogenic leptospires (SPL) for 24 h. Through confocal microscopy, we observed a reduction in TLR2, CD11b, and CD206 (mannose receptor) levels when these cells were treated with SPL or recombinant thermolysin for 24 h. Furthermore, opsonins such as C3b/iC3b deposited on the surface of pathogenic leptospires were clearly degraded in the presence of recombinant thermolysin or recombinant leptolysin. Consequently, when opsonized bacteria and macrophages were previously incubated with these proteases, phagocytic activity was diminished. These observations lead us to suggest that proteases secreted by pathogenic leptospires could degrade opsonins present in normal serum or deposited on the bacterial membrane, as well as cleave or inhibit macrophage surface molecules. Therefore, these proteases could interfere with the recognition and internalization by murine macrophages, favoring the spread of leptospires in the host.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105469"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obituary Tomasz Skirecki (May 22nd, 1987–January 21st, 2025) 托马兹·斯基雷基讣告（1987年5月22日- 2025年1月21日）

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2025.105482

Marcin Osuchowski, Jean-Marc Cavaillon

引用次数: 0

Dopaminergic neuronal regulation determines innate immunity of Caenorhabditis elegans during Klebsiella aerogenes infection 多巴胺能神经元调控决定了草履虫在产气克雷伯氏菌感染期间的先天免疫力。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105430

Gowripriya Thirumugam , Yashwanth Radhakrishnan , James Prabhanand Bhaskar , Suresh Ramamurthi , Balamurugan Krishnaswamy

{"title":"Dopaminergic neuronal regulation determines innate immunity of Caenorhabditis elegans during Klebsiella aerogenes infection","authors":"Gowripriya Thirumugam , Yashwanth Radhakrishnan , James Prabhanand Bhaskar , Suresh Ramamurthi , Balamurugan Krishnaswamy","doi":"10.1016/j.micinf.2024.105430","DOIUrl":"10.1016/j.micinf.2024.105430","url":null,"abstract":"<div><div>The innate immune signals are the front line of host defense against bacterial pathogens. Pathogen-induced harmful effects, such as reduced neuronal signals to the intestine, affect the host's food sensing and dwelling behavior. Here, we report that dopamine and <em>kpc-1</em> signals control the intestinal innate immune responses through the p38/PMK-1 MAPK signaling pathway in <em>C. elegans</em>. <em>K. aerogenes</em> infection in <em>C. elegans</em> affects the food-dwelling behavior, which depends on dopamine regulation. The absence of the dopamine receptor (<em>dop-1</em>) and transporter (<em>dat-1</em>) increases attraction to the pathogen instead of avoidance. The <em>K. aerogenes</em> infection affects <em>age-1</em> regulation through the furin-like proprotein convertase (<em>kpc-1</em>); the absence of <em>kpc-1</em> affects environment-dependent dauer formation. In contrast, the <em>dop-1</em> mutation antagonistically regulates intestinal immune regulation, while the <em>kpc-1</em> mutation partially regulates the p38/PMK-1 MAPK pathway. Our findings indicate that dopamine and <em>kpc-1</em>signaling from the nervous system control intestinal immunity in an antagonistic and agonistic manner, respectively.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105430"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The efficacy of the bacteriocinogenic Enterococcus faecalis 14 in the control of induced necrotic enteritis in broilers 产菌性粪肠球菌14对肉鸡诱导性坏死性肠炎的防治效果。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2025.105477

Rabia Ladjouzi , Bernard Taminiau , Georges Daube , Anca Lucau-Danila , Djamel Drider

{"title":"The efficacy of the bacteriocinogenic Enterococcus faecalis 14 in the control of induced necrotic enteritis in broilers","authors":"Rabia Ladjouzi , Bernard Taminiau , Georges Daube , Anca Lucau-Danila , Djamel Drider","doi":"10.1016/j.micinf.2025.105477","DOIUrl":"10.1016/j.micinf.2025.105477","url":null,"abstract":"<div><h3>Purpose</h3><div>To demonstrate the efficacy of the bacteriocinogenic <em>Enterococcus faecalis</em> 14 (<em>E. faecalis</em> 14) in the control of induced necrotic enteritis (NE) in broilers.</div></div><div><h3>Methods</h3><div>Six groups of 504 broilers consisting of an infected untreated control (IUC) group, an infected and amoxicillin treated control (ITC) group, and groups receiving prophylactically (2 groups) or therapeutically (2 groups) <em>E. faecalis</em> 14 or its Δ<em>bac</em> mutant were used. All groups were challenged with <em>Clostridium perfringens</em> 56 to induce NE. To predispose the boilers to develop subclinical NE, a high protein grower diet containing 15 % fishmeal and a coccidial inoculum were administered.</div></div><div><h3>Results</h3><div>NE lesions were observed on D26 in all groups except ITC and those receiving prophylactically and therapeutically <em>E. faecalis</em> 14. On D27, only ITC and the group prophylactically treated with <em>E. faecalis</em> 14 (T03) were without lesions. Average body weight and daily weight gain remained lower in the treated groups compared to the ITC group, but there was a clear improvement in the period between D21 to D27, especially in the group prophylactically treated with <em>E. faecalis</em> 14. Specifically, the daily weight gain (DWG) in this period for group T03, was second highest after the group ITC. Metataxonomic analyses showed a positive effect of <em>E. faecalis</em> 14 in maintaining the diversity and richness of the intestinal microbiota, in contrast to ITC group and other conditions.</div></div><div><h3>Conclusions</h3><div>The results of this <em>in vivo</em> study demonstrated the efficacy of the prophylactic administration of the bacteriocinogenic <em>E. faecalis</em> 14 in preventing of the NE lesions caused by <em>C. perfringens</em>.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105477"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chytridiomycosis disrupts metabolic responses in amphibians at metamorphic climax 恙虫病扰乱了处于变态高潮期的两栖动物的新陈代谢反应。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105438

Josephine E. Humphries , Steven D. Melvin , Chantal Lanctôt , Hamish McCallum , David Newell , Laura F. Grogan

{"title":"Chytridiomycosis disrupts metabolic responses in amphibians at metamorphic climax","authors":"Josephine E. Humphries , Steven D. Melvin , Chantal Lanctôt , Hamish McCallum , David Newell , Laura F. Grogan","doi":"10.1016/j.micinf.2024.105438","DOIUrl":"10.1016/j.micinf.2024.105438","url":null,"abstract":"<div><div>The fungal disease chytridiomycosis (causative agent <em>Batrachochytrium dendrobatidis</em> [Bd]) is a primary contributor to amphibian species declines. The morphological and physiological reorganization that occurs during amphibian metamorphosis likely increases the vulnerability of metamorphs to Bd. To address this, we exposed pro-metamorphic tadpoles of Fleay's barred frog (<em>Mixophyes fleayi)</em> to Bd and sampled skin and liver sections from control and exposed animals throughout metamorphosis (Gosner stages 40, 42 and 45). We used an untargeted metabolomics approach to assess the metabolic impacts of Bd infection during the critical metamorphic stages, extracting metabolites from sampled tissues and analysing them via Nuclear Magnetic Resonance spectrometry. Most exposed animals became moribund at Gosner stage 45, while a subset seemingly cleared their infections. Metabolite abundance varied throughout development, with Gosner stage 45 samples distinct from previous stages. Clinically infected animals at Gosner stage 45 exhibited profound metabolic dysregulation (e.g., upregulation of amino acid biosynthesis and degradation) in comparison to uninfected groups (negative controls and ‘cleared’ animals). Despite showing parallels with previous metabolomic analyses of Bd-infected adult frogs, we identified variations in our results that could be attributed to the dramatic changes that characterise metamorphosis and may be driving the heightened vulnerability observed in metamorphic amphibians.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105438"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of route of delivery on Chlamydia abortus vaccine-induced immune responses and genital tract immunity in mice 递送途径对流产衣原体疫苗诱导的免疫反应和小鼠生殖道免疫的作用。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105463

Shakyra Richardson , F.N.U. Medhavi , Tayhlor Tanner , Stephanie Lundy , Yusuf Omosun , Joseph U. Igietseme , Francis O. Eko

{"title":"Role of route of delivery on Chlamydia abortus vaccine-induced immune responses and genital tract immunity in mice","authors":"Shakyra Richardson , F.N.U. Medhavi , Tayhlor Tanner , Stephanie Lundy , Yusuf Omosun , Joseph U. Igietseme , Francis O. Eko","doi":"10.1016/j.micinf.2024.105463","DOIUrl":"10.1016/j.micinf.2024.105463","url":null,"abstract":"<div><div>We investigated if the efficacy of a <em>Chlamydia abortus</em> (Cab) subunit vaccine is influenced by route of administration. Thus, female CBA/J mice were immunized either by mucosal or systemic routes with <em>Vibrio cholerae</em> ghost (VCG)-based vaccine expressing T and B cell epitopes of Cab polymorphic membrane protein (Pmp) 18D, termed rVCG-Pmp18.3. Vaccine evaluation revealed that all routes of vaccine delivery induced a Th1-type antibody response after a prime boost or three-dose immunization regimen. Also, the intranasal and rectal mucosal and intramuscular systemic routes induced cross-reactive neutralizing antibodies against homologous and heterologous Cab strains. Irrespective of the route of immunization, the vaccine elicited a Th1-type cytokine response (IFN-γ/IL-4 >1) in immunized mice. Analysis of reduction in genital Cab burden as an index of protection showed that immunization induced substantial degrees of protection against infection, irrespective of route of delivery with the intranasal and rectal mucosal routes showing superior levels of protection 12 days postchallenge. Furthermore, there was correlation between the humoral and cellular immune response and protection was associated with the Cab-specific serum IgG antibody avidity and IFN-γ. Thus, while route of administration impacts vaccine efficacy, the rVCG-Pmp18.3-induced protective immunity against Cab respiratory infection can be accomplished by both mucosal and systemic immunization.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105463"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endogenous retroviruses in neurodevelopmental, psychotic and cognitive disorders. 内源性逆转录病毒在神经发育、精神病和认知障碍中的作用。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-02-04 DOI: 10.1016/j.micinf.2025.105479

Urs Meyer, Iris Katharina Penner

{"title":"Endogenous retroviruses in neurodevelopmental, psychotic and cognitive disorders.","authors":"Urs Meyer, Iris Katharina Penner","doi":"10.1016/j.micinf.2025.105479","DOIUrl":"10.1016/j.micinf.2025.105479","url":null,"abstract":"<p><p>Endogenous retroviruses (ERVs) are inherited retroviral genomic elements that integrated into the mammalian genome through germline infections and insertions during evolution. Human ERVs (HERVs) comprise approximately 8 % of the human genome and are increasingly recognized to be involved in the etiology and pathophysiology of numerous brain disorders. In this narrative review, we summarize the existing evidence linking abnormal HERV expression to neurodevelopmental and psychosis-related disorders and discuss how these retroviral elements may contribute to the heterogeneity in clinical outcomes. We also review the findings suggesting that aberrant HERV expression contribute to late-onset cognitive disorders with neurodegenerative components, such as Alzheimer's disease (AD) and other forms of dementia. The evidence implicating abnormal HERV expression in neurodevelopmental, psychotic, and cognitive disorders is manifold and stems from diverse research fields, including human post-mortem brain studies, serological investigations, gene expression analyses, and clinical trials with HERV-specific pharmacological compounds. The recent establishment and use of animal models offer a complementary experimental platform that will help establish causal relationships and identify specific disease pathways affected by abnormal HERV expression. Yet, significant gaps persist in understanding the role of HERVs in neurodevelopmental, psychotic, and cognitive disorders, particularly concerning the specificity and stability of abnormal HERV expression in these conditions. Addressing these questions appears crucial for optimizing the potential benefits of therapeutic interventions aimed at targeting abnormal HERV expression across the broad spectrum of HERV-associated disorders of the central nervous system.</p>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105479"},"PeriodicalIF":2.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143365055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of immune-associated genes involved in latent Mycobacterium marinum infection 鉴定参与潜伏分枝杆菌感染的免疫相关基因。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-02-01 DOI: 10.1016/j.micinf.2024.105407

Pingping Jia , Shize Peng , Yi Zhang , Jianyuan Zhao , Qianqian Zhao , Xiaoxiao Wu , Fangqi Shen , Kai Sun , Liyan Yu , Shan Cen

{"title":"Identification of immune-associated genes involved in latent Mycobacterium marinum infection","authors":"Pingping Jia , Shize Peng , Yi Zhang , Jianyuan Zhao , Qianqian Zhao , Xiaoxiao Wu , Fangqi Shen , Kai Sun , Liyan Yu , Shan Cen","doi":"10.1016/j.micinf.2024.105407","DOIUrl":"10.1016/j.micinf.2024.105407","url":null,"abstract":"<div><div>Tuberculosis (TB) is a high mortality infectious disease caused by <em>Mycobacterium tuberculosis</em> (Mtb), and often develops into latent infection. About 5～10% of latent infections turn into active tuberculosis when the host immune system becomes deficient. Therefore, exploring the latent infection mechanism of Mtb is pivotal for the prevention and treatment of tuberculosis. We first established the zebrafish latent infection model and the chronic infection model utilizing <em>Mycobacterium marinum</em>, which has the highly similar gene background to Mtb. Using the latent infection model, we characterized the gene expression profiles and found 462 genes expressed differentially in the latent period and chronic tuberculosis infection. These differentially expressed genes are involved in various biological processes including transcription, transcriptional regulation, organism development, and immune responses. Among them, nineteen immune-related genes were found to express differentially in the latent period. By analyzing immune related protein network, the genes in the center of the network, including Nos2b, TNFα, IL1, TNFβ, TLR1, TLR2, and TLR4b, displayed significant deferential expression in latent infection and chronic infection period of zebrafish, suggesting that these genes might play an important role in controlling latent infection of Mtb. Identifying immune biomarker related to the status of tuberculosis latent infection might lead to novel strategy for diagnosis and treatment.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 2","pages":"Article 105407"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microneedle-based arrays – Breakthrough strategy for the treatment of bacterial and fungal skin infections 微针阵列--治疗细菌和真菌皮肤感染的突破性策略。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-02-01 DOI: 10.1016/j.micinf.2024.105426

Oliwia Kordyl , Zuzanna Styrna , Monika Wojtyłko , Bozena Michniak-Kohn , Tomasz Osmałek

{"title":"Microneedle-based arrays – Breakthrough strategy for the treatment of bacterial and fungal skin infections","authors":"Oliwia Kordyl , Zuzanna Styrna , Monika Wojtyłko , Bozena Michniak-Kohn , Tomasz Osmałek","doi":"10.1016/j.micinf.2024.105426","DOIUrl":"10.1016/j.micinf.2024.105426","url":null,"abstract":"<div><div>Currently, fungal and bacterial skin infections rank among the most challenging public health problems due to the increasing prevalence of microorganisms and the development of resistance to available drugs. A major issue in treating these infections with conventional topical medications is the poor penetration through the <em>stratum corneum</em>, the outermost layer of the skin. The concept of microneedles seems to be a future-proof approach for delivering drugs directly into deeper tissues. By bypassing the skin barrier, microneedle systems allow therapeutic substances to reach deeper layers more efficiently, significantly improving treatment outcomes. Nonetheless, the primary challenges regarding the effectiveness of microneedles involve selecting the appropriate size and shape, along with polymer composition and fabrication technology, to enable controlled and efficient drug release. This review offers a comprehensive overview of the latest knowledge on microneedle types and manufacturing techniques, highlighting their potential effectiveness in treating bacterial and fungal skin infections. It includes updated statistics on infection prevalence and provides a detailed examination of common bacterial and fungal diseases, focusing on their symptoms, causative species, and treatment methods. Additionally, the review addresses safety considerations, regulatory aspects, and future perspectives for microneedle-based therapeutic systems. It also underscores the importance of industrialization and clinical translation efforts, emphasizing the significant potential of microneedle technology for advancing medical applications.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 2","pages":"Article 105426"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0