Microbes and Infection最新文献

{"title":"Characterization and dynamics of intestinal microbiota in patients with Clostridioides difficile colonization and infection","authors":"Yanzi Zhou ,&nbsp;Lihua Guo ,&nbsp;Tingting Xiao ,&nbsp;Yunbo Chen ,&nbsp;Tao Lv ,&nbsp;Yuan Wang ,&nbsp;Shuntian Zhang ,&nbsp;Hongliu Cai ,&nbsp;Xiaohui Chi ,&nbsp;Xiaoyang Kong ,&nbsp;Kai Zhou ,&nbsp;Ping Shen ,&nbsp;Yonghong Xiao","doi":"10.1016/j.micinf.2024.105373","DOIUrl":"10.1016/j.micinf.2024.105373","url":null,"abstract":"<div><div>Gut microbiota dysbiosis increases the susceptibility to <em>Clostridioides difficile</em> infection (CDI). In this study, we monitored <em>C. difficile</em> colonization (CDC) patients from no CDC status (CDN) to CDC status (CDCp) and CDI patients from asymptomatic status before CDI (PRECDI), CDI status (ONCDI), to asymptomatic status after CDI (POSTCDI). Based on metagenomic sequencing, we aimed to investigate the interaction pattern between gut microbiota and <em>C. difficile</em>. There was no significant difference of microbiota diversity between CDN and CDCp. In CDCp, Bacteroidetes and short-chain fatty acid (SCFA)-producing bacteria increased, with a positive correlation between SCFA-producing bacteria and <em>C. difficile</em> colonization. Compared with PRECDI, ONCDI and POSTCDI showed a significant decrease in microbiota diversity, particularly in Bacteroidetes and SCFA-producing bacteria, with a positive correlation between opportunistic pathogen and <em>C. difficile</em>. Fatty acid metabolism, and amino acid biosynthesis were enriched in CDN, CDCp, and PRECDI, while bile secretion was enriched in ONCDI and POSTCDI. Microbiota and metabolic pathways interaction networks in CDN and CDCp were more complex, particularly pathways in fatty acid and bile acid metabolism. Increasing of Bacteroidetes and SCFA-producing bacteria, affecting amino acid and fatty acid metabolism, is associated with colonization resistance to <em>C. difficile</em> and inhibiting the development of CDI.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105373"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of microRNAs in immune regulation and pathogenesis of Chlamydia trachomatis and Chlamydia muridarum infections: a rapid review","authors":"Chloe Meewes,&nbsp;Kanupriya Gupta,&nbsp;William M. Geisler","doi":"10.1016/j.micinf.2024.105397","DOIUrl":"10.1016/j.micinf.2024.105397","url":null,"abstract":"<div><div>MicroRNAs in <em>Chlamydia trachomatis</em> (CT) and <em>Chlamydia muridarum</em> (CM) infections are an emerging topic of research that provide knowledge that could advance vaccine development and strategies for managing infection. This rapid review summarizes human and murine studies on miRNA expression in CT and CM infections in vivo and ex vivo.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105397"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141723898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated analysis of microbiome and host transcriptome unveils correlations between lung microbiota and host immunity in bronchoalveolar lavage fluid of pneumocystis pneumonia patients","authors":"Ling Zhang ,&nbsp;Miaotian Cai ,&nbsp;Xin Zhang ,&nbsp;Sitong Wang ,&nbsp;Lijun Pang ,&nbsp;Xue Chen ,&nbsp;Caopei Zheng ,&nbsp;Yuqing Sun ,&nbsp;Ying Liang ,&nbsp;Shan Guo ,&nbsp;Feili Wei ,&nbsp;Yulin Zhang","doi":"10.1016/j.micinf.2024.105374","DOIUrl":"10.1016/j.micinf.2024.105374","url":null,"abstract":"<div><h3>Objective</h3><div><span><span>The lung microbiota of patients with </span>pulmonary diseases<span> is disrupted and impacts the immunity. The microbiological and immune landscape of the lungs in patients with </span></span>pneumocystis pneumonia (PCP) remains poorly understood.</div></div><div><h3>Methods</h3><div><span>Multi-omics analysis and machine learning were performed on bronchoalveolar lavage fluid<span> to explore interaction between the lung microbiota and host immunity in PCP. Then we constructed a diagnostic model using differential genes with LASSO regression and validated by </span></span>qPCR<span>. The immune infiltration analysis was performed to explore the landscape of lung immunity in patients with PCP.</span></div></div><div><h3>Results</h3><div><span>Patients with PCP showed a low alpha diversity of lung microbiota, accompanied by the elevated abundance of </span><span><span>Firmicutes</span></span><span><span>, and the differential expressed genes (DEGs) analysis displayed a downregulation of </span>MAPK signaling<span><span>. The MAPK10, TGFB1, and </span>EFNA3 indicated a potential to predict PCP (AUC = 0.86). The lung immune landscape in PCP showed the lower levels of naïve CD4</span></span>⁺<span> T cells<span> and activated dendritic cells. The correlation analysis of the MAPK signaling pathway-related DEGs and the differential microorganisms at the level of phylum showed that the </span></span><span><span>Firmicutes</span></span> was negatively correlated with these DEGs.</div></div><div><h3>Conclusion</h3><div>We profiled the characteristics of lung microbiota and immune landscape in PCP, which may contribute to elucidating the mechanism of PCP.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105374"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophage-depleted young mice are beneficial in vivo models to assess the translocation of Klebsiella pneumonia from the gastrointestinal tract to the liver in the elderly","authors":"Hitoshi Tsugawa ,&nbsp;Shogo Tsubaki ,&nbsp;Rika Tanaka ,&nbsp;Sho Nashimoto ,&nbsp;Jin Imai ,&nbsp;Juntaro Matsuzaki ,&nbsp;Katsuto Hozumi","doi":"10.1016/j.micinf.2024.105371","DOIUrl":"10.1016/j.micinf.2024.105371","url":null,"abstract":"<div><div>Pathobionts are commensal intestinal microbiota capable of causing systemic infections under specific conditions, such as environmental changes or aging. However, it is unclear how pathobionts are recognized by the intestinal mucosal immune system under physiological conditions. This study demonstrates that the gut pathobiont <em>Klebsiella pneumoniae</em> causes injury to the epithelium and translocates to the liver in specific pathogen-free mice treated with clodronate-liposomes that depleted macrophages. In the clodronate-liposome-treated mice, indigenous classical <em>K. pneumoniae</em> (cKp) with non-K1/K2 capsular serotypes were isolated from the liver, indicating that gut commensal cKp translocated from the gastrointestinal tract to the liver due to the depletion of intestinal macrophages. Oral inoculation of isolated cKp to clodronate-liposome-treated mice significantly reduced the survival rates compared to that of non-treated mice. Our findings demonstrate that intestinal mucosal macrophages play a pivotal role in sensing commensal cKp and suppressing their translocation to the liver. This study demonstrates that clodronate-liposome-treated mouse models are effective for screening and evaluating drugs that prevent the translocation of cKp to the liver, providing new insights into the development of preventive protocols against <em>K. pneumoniae</em> infection.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105371"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Acinetobacter baumannii at a tertiary hospital in Guangzhou: a genomic and clinical study","authors":"Heng Heng ,&nbsp;Ling Yang ,&nbsp;Zhiwei Zheng ,&nbsp;Chen Yang ,&nbsp;Xuemei Yang ,&nbsp;Wenxing Zhao ,&nbsp;Ruanyang Sun ,&nbsp;Kaichao Chen ,&nbsp;Lianwei Ye ,&nbsp;Jun Li ,&nbsp;Edward Wai-Chi Chan ,&nbsp;Sheng Chen","doi":"10.1016/j.micinf.2024.105380","DOIUrl":"10.1016/j.micinf.2024.105380","url":null,"abstract":"<div><div><span><em>Acinetobacter baumannii</em></span><span><span> (AB) infections have become a global public health<span> concern due to the continued increase in the incidence of infection and the rate of resistance to carbapenems. This study aimed to investigate the genomic features of </span></span>AB<span> strains recovered from a tertiary hospital and assess the clinical implications of the findings. A total of 217 AB strains were collected between 2016 and 2018 at a tertiary hospital in Guangzhou, with 183 (84.33%) being carbapenem-resistant AB (CRAB), with the main mechanism being the carriage of the </span></span><em>bla</em>_OXA-23<span> gene. The overall mortality rate<span><span> of patients caused by such strains was 15.21% (n = 33). Artificial lung ventilation and the use of </span>meropenem<span><span> were mortality risk factors in AB-infected patients, while KL2 AB infection was negatively associated. Core genome multilocus sequence typing and clustering analysis were performed on the integrated AB genome collection from the NCBI database and this study to illustrate the population structure among China. The results revealed diverse core genome profiles (n = 17) among AB strains from China, and strains from this single hospital exhibited most of the core genome profiles (n = 13), suggesting genetic variability within the hospital and transmission across the country. These findings show that the high transmission potential of the CRAB strains and </span>meropenem<span> usage that confers a selective advantage of CRAB clinically are two major factors that pose significant challenges to the effective clinical management of AB infections. Understanding the genetic features and transmission patterns of clinical AB strains is crucial for the effective control of infections caused by this pathogen.</span></span></span></span></div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105380"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141443059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomolecular condensates with liquid properties formed during viral infections","authors":"Damien Glon,&nbsp;Benjamin Léonardon,&nbsp;Ariane Guillemot,&nbsp;Aurélie Albertini,&nbsp;Cécile Lagaudrière-Gesbert,&nbsp;Yves Gaudin","doi":"10.1016/j.micinf.2024.105402","DOIUrl":"10.1016/j.micinf.2024.105402","url":null,"abstract":"<div><div>During a viral infection, several membraneless compartments with liquid properties are formed. They can be of viral origin concentrating viral proteins and nucleic acids, and harboring essential stages of the viral cycle, or of cellular origin containing components involved in innate immunity. This is a paradigm shift in our understanding of viral replication and the interaction between viruses and innate cellular immunity.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105402"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymicrobial consortia in the pathogenesis of biofilm vaginosis visualized by FISH. Historic review outlining the basic principles of the polymicrobial infection theory","authors":"Alexander Swidsinski ,&nbsp;Rudolf Amann ,&nbsp;Alexander Guschin ,&nbsp;Sonja Swidsinski ,&nbsp;Vera Loening-Baucke ,&nbsp;Werner Mendling ,&nbsp;Jack D. Sobel ,&nbsp;Ronald F. Lamont ,&nbsp;Mario Vaneechoutte ,&nbsp;Pedro Vieira Baptista ,&nbsp;Catriona S. Bradshaw ,&nbsp;Igor Yu Kogan ,&nbsp;Аlevtina M. Savicheva ,&nbsp;Oleg V. Mitrokhin ,&nbsp;Nadezhda W. Swidsinski ,&nbsp;Gennadiy T. Sukhikh ,&nbsp;Tatjana V. Priputnevich ,&nbsp;Inna A. Apolikhina ,&nbsp;Yvonne Dörffel","doi":"10.1016/j.micinf.2024.105403","DOIUrl":"10.1016/j.micinf.2024.105403","url":null,"abstract":"<div><div>The manuscript disputes the exclusive mono-infectious way of thinking, which presumes that for every infection only one pathogen is responsible and sufficient, when infectious vectors, close contact and reduced immunity meet. In situations involving heavily colonized anatomical sites such an approach often ends in insoluble contradictions. Upon critical reflection and evaluation of 20 years research on spatial organization of vaginal microbiota it is apparent, that in some situations, pathogens may act and operate in permanent, structurally organized consortia, whereas its individual components may be innocuous and innocent, failing to express any pathogenic effect. In these cases, consortia are the true pathogens responsible for many infectious conditions, which usually remain unrecognized as long as improperly diagnosed.</div><div>The structure of such consortia can be unraveled using ribosomal fluorescence in situ hybridization (FISH). FISH methodology, that not only offers an ex vivo opportunity to recognize bacterial species, but provides unique physical insight into their specific role in the pathogenesis of polymicrobial infections. Ribosomal FISH technique applied to both, women with bacterial vaginosis (BV) and their male partners, has added significantly to our understanding of the pathogenesis of this condition and contributed to appreciating the mechanisms of polymicrobial, community-based infection, potentially leading to therapeutic advances.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105403"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141913285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bordetella pertussis outer membrane vesicles impair neutrophil bactericidal activity","authors":"Jimena Alvarez Hayes ,&nbsp;Bruno Blancá ,&nbsp;Juan Pablo Gorgojo,&nbsp;Carlos Baroli,&nbsp;Mariela del Carmen Carrica,&nbsp;Maria Eugenia Rodriguez","doi":"10.1016/j.micinf.2024.105375","DOIUrl":"10.1016/j.micinf.2024.105375","url":null,"abstract":"<div><div><span><span><span>Neutrophils constitute the primary defense against bacterial infections, yet certain pathogens express </span>virulence factors that enable them to subvert neutrophils-mediated killing. Outer </span>membrane vesicles (OMVs) have emerged as a secretory system through which bacteria deliver virulence factors to host cells. OMVs from </span><span><em>Bordetella pertussis</em></span><span>, the etiological agent of whooping cough, are loaded with most of bacterial virulence factors, including CyaA, which plays a key role in </span><em>B. pertussis</em><span> evasion of neutrophils bactericidal activity. In our study, we investigated the role of </span><em>B. pertussis</em> OMVs in bacterial interaction with neutrophils. We observed that interaction of OMVs with neutrophils led to a decrease in the expression of cell surface CR3 and FcγRs, an effect dependent on the CyaA toxin delivered by these vesicles. This decreased receptor expression led to reduced bacterial uptake by neutrophils, irrespective of the presence of opsonic antibodies. Moreover, CyaA delivered by OMVs hindered intracellular bactericidal trafficking, promoting bacterial intracellular survival. When both bacteria and OMVs were opsonized, competition between opsonized OMVs and <em>B. pertussis</em> for FcγRs on neutrophils led to a significant decrease in bacterial uptake. Overall, our findings suggest that <em>B. pertussis</em><span> OMVs promote bacterial survival to the encounter with neutrophils in both naïve and immunized individuals.</span></div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105375"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"C1q modulation of antibody-dependent enhancement of dengue virus infection in human myeloid cell lines is dependent on cell type and antibody specificity","authors":"Alana B. Byrne ,&nbsp;Florencia A. Bonnin ,&nbsp;Eduardo L. López ,&nbsp;Fernando P. Polack ,&nbsp;Laura B. Talarico","doi":"10.1016/j.micinf.2024.105378","DOIUrl":"10.1016/j.micinf.2024.105378","url":null,"abstract":"<div><div><span><span>Antibody-dependent enhancement (ADE) of dengue virus (DENV) infection is one of the mechanisms contributing to increased severity during heterotypic, secondary infection. The complement protein </span>C1q has been shown to reduce the magnitude of ADE </span><em>in vitro</em>. Therefore, we investigated the mechanisms of C1q modulation of ADE, focusing on processes of viral entry.</div><div><span>Using a model of ADE of DENV-1 infection in human myeloid cell lines<span> in the presence of monoclonal antibodies, 4G2 and 2H2, we found that C1q produced nearly a 40-fold reduction of ADE of DENV-1 in K562 cells, but had no effect in U937 cells. In K562 cells, C1q reduced adsorption of DENV-1/4G2 and exerted a dual inhibitory effect on adsorption and </span></span>internalization of DENV-1/2H2. Distinct endocytic pathways in the presence of antibody corresponded to conditions where C1q produced a differential action. Also, C1q did not affect the intrinsic cell response mediated by FcγR in human myeloid cells.</div><div><span>The modulation of ADE of DENV-1 by C1q is dependent on the FcγR expressed on immune cells and the specificity of the antibody comprising the immune complex. Understanding protective and pathogenic mechanisms in the </span>humoral response to DENV infections is crucial for the successful design of antivirals and vaccines.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105378"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141331400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of tuberculosis-specific antigen stimulation on the diagnostic accuracy of interferon-γ inducible protein-10 in distinguishing active and latent tuberculosis infection: a meta-analysis","authors":"Muhammad Iqhrammullah ,&nbsp;Rika Yusnaini ,&nbsp;Shakira Amirah ,&nbsp;Intan Chaharunia Mulya ,&nbsp;Ghina Tsurayya ,&nbsp;Muhammad Alif Naufal ,&nbsp;Sukmawan Fajar Santosa ,&nbsp;Harapan Harapan ,&nbsp;Baidillah Zulkifli","doi":"10.1016/j.micinf.2024.105396","DOIUrl":"10.1016/j.micinf.2024.105396","url":null,"abstract":"<div><h3>Background</h3><div>Identifying active tuberculosis (ATB) from latent tuberculosis infection (LTBI) persists as a challenge, and interferon-γ inducible protein-10 (IP-10) has been employed as the solution. To further improve its diagnostic performance, the sample can be stimulated with TB specific antigen (TBAg).</div></div><div><h3>Aim</h3><div>To perform meta-analysis on diagnostic accuracy of unstimulated and TBAg-stimulated IP-10 in differentiating ATB from LTBI.</div></div><div><h3>Methods</h3><div>Systematic search was performed on five major scientific databases as of 29 November 2023. Observational studies reporting diagnostic values of unstimulated or TBAg-stimulated IP-10 in identifying ATB from LTBI were included. Meta-analysis was carried out using two-level mixed-effect logistic regression model.</div></div><div><h3>Results</h3><div>Twenty-five studies recruiting 2301 patients (1137 ATB versus 1164 LTBI) were included in the quantitative analysis. The pooled sensitivity and specifity of IP-10 were 72% (95%CI: 0.59–0.82) and 78% (95%CI: 0.63–0.88), respectively. As for TBAg-stimulated IP-10, the sensitivity and specifity were 82% (95%CI: 0.76–0.87) and 85% (95%CI: 0.73–0.92), respectively. The senstivity was reduced signiticantly (<em>p</em> &lt; 0.01) when the patients with human immunodeficiency virus infection were included, except after the TBAg stimulation.</div></div><div><h3>Conclusion</h3><div>Stimulating IP-10 with TBAg could improve the diagnostic accuracy in differentiating ATB from LTBI.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105396"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141734625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}