Microbes and Infection最新文献

Proteolytic activity of secreted proteases from pathogenic leptospires and effects on phagocytosis by murine macrophages.

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-04 DOI: 10.1016/j.micinf.2025.105469

Thais A Amamura, Daniella Dos S Courrol, Angela S Barbosa, Ildefonso A Silva-Junior, Tiago F da Silva, Leonardo M Midon, Mario C Cruz, Marcos B Heinemann, Rosa M Chura-Chambi, Ligia Morganti, Lourdes Isaac

{"title":"Proteolytic activity of secreted proteases from pathogenic leptospires and effects on phagocytosis by murine macrophages.","authors":"Thais A Amamura, Daniella Dos S Courrol, Angela S Barbosa, Ildefonso A Silva-Junior, Tiago F da Silva, Leonardo M Midon, Mario C Cruz, Marcos B Heinemann, Rosa M Chura-Chambi, Ligia Morganti, Lourdes Isaac","doi":"10.1016/j.micinf.2025.105469","DOIUrl":"https://doi.org/10.1016/j.micinf.2025.105469","url":null,"abstract":"Leptospirosis is a zoonosis caused by spirochete Leptospira. Pathogenic leptospires evade the Complement System, enabling their survival upon contact with normal human serum in vitro. In a previous study, we demonstrated that proteases secreted by pathogenic leptospires cleave several Complement proteins, including C3 and the opsonins C3b and iC3b. We hypothesize that these Leptospira proteases, such as thermolysin and leptolysin, may decrease the phagocytic activity of murine peritoneal macrophages. We observed decreased amounts of CR3 and CR4 using flow cytometry when these cells were treated with supernatant from the culture of pathogenic leptospires (SPL) for 24 h. Through confocal microscopy, we observed a reduction in TLR2, CD11b, and CD206 (mannose receptor) levels when these cells were treated with SPL or recombinant thermolysin for 24 h. Furthermore, opsonins such as C3b/iC3b deposited on the surface of pathogenic leptospires were clearly degraded in the presence of recombinant thermolysin or recombinant leptolysin. Consequently, when opsonized bacteria and macrophages were previously incubated with these proteases, phagocytic activity was diminished. These observations lead us to suggest that proteases secreted by pathogenic leptospires could degrade opsonins present in normal serum or deposited on the bacterial membrane, as well as cleave or inhibit macrophage surface molecules. Therefore, these proteases could interfere with the recognition and internalization by murine macrophages, favoring the spread of leptospires in the host.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105469"},"PeriodicalIF":2.6,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gingivitis- and periodontitis-associated microbiota in bovine deciduous incisor teeth - A preliminary study.

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-12-30 DOI: 10.1016/j.micinf.2024.105468

Juliana Vaccari, Ana C Borsanelli, Flávia R F Athayde, Júlia R Saraiva, Thamiris N M Ramos, Iveraldo S Dutra

{"title":"Gingivitis- and periodontitis-associated microbiota in bovine deciduous incisor teeth - A preliminary study.","authors":"Juliana Vaccari, Ana C Borsanelli, Flávia R F Athayde, Júlia R Saraiva, Thamiris N M Ramos, Iveraldo S Dutra","doi":"10.1016/j.micinf.2024.105468","DOIUrl":"10.1016/j.micinf.2024.105468","url":null,"abstract":"As ruminants are frequently affected by periodontal diseases, understanding their microbial communities is crucial. In this pilot study, we analyzed subgingival biofilm samples of young cattle across different states: clinically healthy (n = 5), gingivitis (n = 5), and periodontitis (n = 5) using 16S rRNA gene sequencing and co-occurrence network analysis. The findings revealed that Proteobacteria was the predominant phylum across all conditions, with Fusobacteriota constituting 27.6 % of the microbiota in periodontitis-affected sites. In healthy sites, Moraxella (21.11 %), Neisseria (13.16 %), and Lautropia (7.69 %) were the predominant genera; in gingivitis-affected sites, the leading genera were Neisseria (23.65 %), Moraxella (18.95 %), and Conchiformibius (10.79 %); and in periodontitis sites, Caviibacter (19.78 %), Moraxella (16.13 %), and Fusobacterium (7.56 %) were most prevalent. Richness and dissimilarity analyses did not show significant differences across the clinical states, but differences were found between gingivitis and periodontitis sites (p = 0.01) in diversity. The co-occurrence networks highlighted significant variances in the central phyla across the phenotypes, with a higher number of positive interactions observed in periodontitis-affected sites. Consequently, this study demonstrated that the microbiota associated with periodontitis in young cattle exhibits greater diversity compared to gingivitis. Notably, in the deciduous dentition of cattle, the genera Caviibacter and Moraxella are pivotal in the context of periodontitis and periodontal health, respectively.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105468"},"PeriodicalIF":2.6,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting the concept of giant viruses.

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-12-24 DOI: 10.1016/j.micinf.2024.105467

Jônatas Santos Abrahão

引用次数: 0

HERV reactivation by adenovirus infection is associated with viral immune regulation. "腺病毒感染导致的 HERV 再激活与病毒免疫调节有关"。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-12-21 DOI: 10.1016/j.micinf.2024.105466

Wen Liang, Miona Stubbe, Lisa Pleninger, Anna Hofferek, Hans Stubbe, Julia Mai, Salih Özer, Dmitrij Frishman, Sabrina Schreiner, Michelle Vincendeau

引用次数: 0

Endogenous retroelement expression in modeled airway epithelial repair.

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-12-15 DOI: 10.1016/j.micinf.2024.105465

Stephanie Michael, Nicholas Liotta, Tongyi Fei, Matthew L Bendall, Douglas F Nixon, Nicholas Dopkins

{"title":"Endogenous retroelement expression in modeled airway epithelial repair.","authors":"Stephanie Michael, Nicholas Liotta, Tongyi Fei, Matthew L Bendall, Douglas F Nixon, Nicholas Dopkins","doi":"10.1016/j.micinf.2024.105465","DOIUrl":"10.1016/j.micinf.2024.105465","url":null,"abstract":"Cystic fibrosis (CF) is an autosomal recessive genetic disorder characterized by impairment of the CF transmembrane conductance regulator (CFTR) via gene mutation. CFTR is expressed at the cellular membrane of epithelial cells and functions as an anion pump which maintains water and salt ion homeostasis. In pulmonary airways of CF patients, pathogens such as P. aeruginosa and subsequent uncontrolled inflammation damage the human airway epithelial cells (HAECs) and can be life-threatening. We previously identified that inhibiting endogenous retroelement (ERE) reverse transcriptase can hamper the inflammatory response to bacterial flagella in THP-1 cells. Here, we investigate how ERE expression is sensitive to HAEC repair and toll-like receptor 5 (TLR5) activation, a primary mechanism by which inflammation impacts disease outcome. Our results demonstrate that several human endogenous retroviruses (HERVs) and long interspersed nuclear elements (LINEs) fluctuate throughout the various stages of repair and that TLR5 activation further influences ERE expression. By considering the impact of the most common CF mutation F508del/F508del on ERE expression in unwounded HAECs, we also found that two specific EREs, L1FLnI_2p23.1c and HERVH_10p12.33, were downregulated in CF-derived HAECs. Collectively, we show that ERE expression in HAECs is sensitive to certain modalities reflective of CF pathogenesis, and specific EREs may be indicative of CF disease state and pathogenesis.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105465"},"PeriodicalIF":2.6,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142837495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The SARS-CoV-2 antibody-dependent enhancement façade.

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-12-09 DOI: 10.1016/j.micinf.2024.105464

Jeremia M Coish, Lori A MacNeil, Adam J MacNeil

{"title":"The SARS-CoV-2 antibody-dependent enhancement façade.","authors":"Jeremia M Coish, Lori A MacNeil, Adam J MacNeil","doi":"10.1016/j.micinf.2024.105464","DOIUrl":"10.1016/j.micinf.2024.105464","url":null,"abstract":"Antibody-dependent enhancement (ADE) is an immunological paradox whereby sensitization following a primary viral infection results in the subsequent enhancement of a similar secondary infection. This idiosyncratic immune response has been established in dengue virus infections, driven by four antigenically related serotypes co-circulating in endemic regions. Several coronaviruses exhibit antibody-mediated mechanisms of viral entry, which has led to speculation of an ADE capacity for SARS-CoV-2, though in vivo and epidemiological evidence do not currently support this phenomenon. Three distinct antibody-dependent mechanisms for SARS-CoV-2 entry have recently been demonstrated: 1. FcR-dependent, 2. ACE2-FcR-interdependent, and 3. FcR-independent. These mechanisms of viral entry may be dependent on SARS-CoV-2 antibody specificity; antibodies targeting the receptor binding domain (RBD) typically result in Fc-dependent and ACE2-FcR-interdependent entry, whereas antibodies targeting the N-terminal domain can induce a conformational change to the RBD that optimizes ACE2-receptor binding domain interactions independent of Fc receptors. Whether these antibody-dependent entry mechanisms of SARS-CoV-2 result in the generation of infectious progenies and enhancement of infection has not been robustly demonstrated. Furthermore, ADE of SARS-CoV-2 mediated by antigenic seniority remains a theoretical concern, as no evidence suggests that SARS-CoV-2 imprinting blunts a subsequent immune response, contributing to severe COVID-19 disease.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105464"},"PeriodicalIF":2.6,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution and zoonotic risk of O1:K1 and O2:K1 avian pathogenic Escherichia coli.

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-12-05 DOI: 10.1016/j.micinf.2024.105462

Eun-Jin Ha, Seung-Min Hong, Kang-Seuk Choi, Hyuk-Joon Kwon

{"title":"Evolution and zoonotic risk of O1:K1 and O2:K1 avian pathogenic Escherichia coli.","authors":"Eun-Jin Ha, Seung-Min Hong, Kang-Seuk Choi, Hyuk-Joon Kwon","doi":"10.1016/j.micinf.2024.105462","DOIUrl":"10.1016/j.micinf.2024.105462","url":null,"abstract":"The O1 and O2 serogroups of avian pathogenic E. coli (APEC) and human extraintestinal pathogenic E. coli (huExPEC) are closely related, but their evolutionary relationships need to be further elucidated. This study classified nineteen O1 and O2 APEC into rpoB sequence types (RSTs) and compared them with reference huExPEC using molecular prophage typing, virulence and antibiotic resistance gene profiling, and comparative genomics. Most O1:K1 and O2:K1 APEC (73.7 %) were classified as RST46-1 and RST47-9. RST47-9 is unique to Korean O1 APEC and likely derives from RST46-1 APEC. The six APEC showed high genome coverage/identity with the Korean RST46-1 huExPEC. Based on RST network and comparative genomics, we hypothesized that the O1 antigen first appeared in RST19-1 and O2 in RST24-1 E. coli in humans. Then, O1 and O2-antigen horizontally transferred to human RST46-1, where a unique K1 capsule (K1-cps) first appeared. The Korean APEC and huExPEC share evolutionary CRISPR spacers but differ in molecular antibiograms and prophage contents. Thus, RST46-1 huExPEC transmitted and evolved in poultry. The zoonotic risks remain unknown, but the substantial virulence of the RST46-1 APEC indicates that the reverse zoonotic risk of huExPEC in poultry is alarming.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105462"},"PeriodicalIF":2.6,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of route of delivery on Chlamydia abortus vaccine-induced immune responses and genital tract immunity in mice.

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-12-05 DOI: 10.1016/j.micinf.2024.105463

Shakyra Richardson, F N U Medhavi, Tayhlor Tanner, Stephanie Lundy, Yusuf Omosun, Joseph U Igietseme, Francis O Eko

{"title":"Role of route of delivery on Chlamydia abortus vaccine-induced immune responses and genital tract immunity in mice.","authors":"Shakyra Richardson, F N U Medhavi, Tayhlor Tanner, Stephanie Lundy, Yusuf Omosun, Joseph U Igietseme, Francis O Eko","doi":"10.1016/j.micinf.2024.105463","DOIUrl":"10.1016/j.micinf.2024.105463","url":null,"abstract":"We investigated if the efficacy of a Chlamydia abortus (Cab) subunit vaccine is influenced by route of administration. Thus, female CBA/J mice were immunized either by mucosal or systemic routes with Vibrio cholerae ghost (VCG)-based vaccine expressing T and B cell epitopes of Cab polymorphic membrane protein (Pmp) 18D, termed rVCG-Pmp18.3. Vaccine evaluation revealed that all routes of vaccine delivery induced a Th1-type antibody response after a prime boost or three-dose immunization regimen. Also, the intranasal and rectal mucosal and intramuscular systemic routes induced cross-reactive neutralizing antibodies against homologous and heterologous Cab strains. Irrespective of the route of immunization, the vaccine elicited a Th1-type cytokine response (IFN-γ/IL-4 >1) in immunized mice. Analysis of reduction in genital Cab burden as an index of protection showed that immunization induced substantial degrees of protection against infection, irrespective of route of delivery with the intranasal and rectal mucosal routes showing superior levels of protection 12 days postchallenge. Furthermore, there was correlation between the humoral and cellular immune response and protection was associated with the Cab-specific serum IgG antibody avidity and IFN-γ. Thus, while route of administration impacts vaccine efficacy, the rVCG-Pmp18.3-induced protective immunity against Cab respiratory infection can be accomplished by both mucosal and systemic immunization.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105463"},"PeriodicalIF":2.6,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of a monoclonal antibody targeting the SARS-CoV-2 Omicron spike protein and analysis of SARS-CoV-2 Omicron mutations related to monoclonal antibody resistance. 生产针对 SARS-CoV-2 Omicron 穗蛋白的单克隆抗体，分析与单克隆抗体抗性有关的 SARS-CoV-2 Omicron 突变。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-21 DOI: 10.1016/j.micinf.2024.105461

Jinsoo Kim, Suyeon Kim, Sangkyu Park, Dongbum Kim, Minyoung Kim, Kyeongbin Baek, Bo Min Kang, Ha-Eun Shin, Myeong-Heon Lee, Younghee Lee, Hyung-Joo Kwon

{"title":"Production of a monoclonal antibody targeting the SARS-CoV-2 Omicron spike protein and analysis of SARS-CoV-2 Omicron mutations related to monoclonal antibody resistance.","authors":"Jinsoo Kim, Suyeon Kim, Sangkyu Park, Dongbum Kim, Minyoung Kim, Kyeongbin Baek, Bo Min Kang, Ha-Eun Shin, Myeong-Heon Lee, Younghee Lee, Hyung-Joo Kwon","doi":"10.1016/j.micinf.2024.105461","DOIUrl":"10.1016/j.micinf.2024.105461","url":null,"abstract":"SARS-CoV-2 mutations have resulted in the emergence of multiple concerning variants, with Omicron being the dominant strain presently. Therefore, we developed a monoclonal antibody (mAb) against the spike (S) protein of SARS-CoV-2 Omicron for therapeutic applications. We established the 1E3H12 mAb, recognizing the receptor binding domain (RBD) of the Omicron S protein, and found that the 1E3H12 mAb can efficiently recognize the Omicron S protein with weak affinity to the Alpha, Beta, and Mu variants, but not to the parental strain and Delta variant. Based on in vitro assays, the mAb demonstrated neutralizing activity against Omicron BA.1, BA.4/5, BQ.1.1, and XBB. A humanized antibody was further produced and proved to have neutralizing activity. To verify the potential limitations of the 1E3H12 mAb due to viral escape of SARS-CoV-2 Omicron variants, we analyzed the emergence of variants by whole genome deep sequencing after serial passage in cell culture. The results showed a few unique S protein mutations in the genome associated with resistance to the mAb. These findings suggest that this antibody not only contributes to the therapeutic arsenal against COVID-19 but also addresses the ongoing challenge of antibody resistance among the evolving subvariants of SARS-CoV-2 Omicron.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105461"},"PeriodicalIF":2.6,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HERV-W envelope protein is present in microglial cells of the human glioma tumor microenvironment and differentially modulates neoplastic cell behavior. HERV-W 包膜蛋白存在于人类胶质瘤肿瘤微环境的小胶质细胞中，并对肿瘤细胞的行为起着不同的调节作用。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-20 DOI: 10.1016/j.micinf.2024.105460

Laura Reiche, Benedikt Plaack, Maike Lehmkuhl, Vivien Weyers, Joel Gruchot, Daniel Picard, Hervé Perron, Marc Remke, Christiane Knobbe-Thomsen, Guido Reifenberger, Patrick Küry, David Kremer

{"title":"HERV-W envelope protein is present in microglial cells of the human glioma tumor microenvironment and differentially modulates neoplastic cell behavior.","authors":"Laura Reiche, Benedikt Plaack, Maike Lehmkuhl, Vivien Weyers, Joel Gruchot, Daniel Picard, Hervé Perron, Marc Remke, Christiane Knobbe-Thomsen, Guido Reifenberger, Patrick Küry, David Kremer","doi":"10.1016/j.micinf.2024.105460","DOIUrl":"10.1016/j.micinf.2024.105460","url":null,"abstract":"Gliomas are the most common parenchymal tumors of the central nervous system (CNS). With regard to their still unclear etiology, several recent studies have provided evidence of a new category of pathogenic elements called human endogenous retroviruses (HERVs) which seem to contribute to the evolution and progression of many neurological diseases such as amyotrophic lateral sclerosis (ALS), schizophrenia, chronic inflammatory polyneuropathy (CIDP) and, particularly, multiple sclerosis (MS). In these diseases, HERVs exert effects on cellular processes such as inflammation, proliferation, and migration. In previous studies, we demonstrated that in MS, the human endogenous retrovirus type-W envelope protein (HERV-W ENV) interferes with lesion repair through the activation of microglia (MG), the innate myeloid immune cells of the CNS. Here, we now show that HERV-W ENV is also present in the microglial cells (MG) of the tumor microenvironment (TME) in gliomas. It modulates the behavior of glioblastoma (GBM) cell lines in GBM/MG cocultures by altering their gene expression, secreted cytokines, morphology, proliferation, and migration properties and could thereby contribute to key tumor properties.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105460"},"PeriodicalIF":2.6,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0