Microbes and Infection最新文献

Intranasal immunization with poly I:C and CpG ODN adjuvants enhances the protective efficacy against Helicobacter pylori infection in mice. 使用聚 I:C 和 CpG ODN 佐剂进行鼻内免疫可增强小鼠对幽门螺旋杆菌感染的保护效力。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-10-24 DOI: 10.1016/j.micinf.2024.105433

Min Sun, Yu Liu, Xiumei Ni, Runqing Tan, Yi Wang, Yajun Jiang, Dingxin Ke, Han Du, Gang Guo, Kaiyun Liu

{"title":"Intranasal immunization with poly I:C and CpG ODN adjuvants enhances the protective efficacy against Helicobacter pylori infection in mice.","authors":"Min Sun, Yu Liu, Xiumei Ni, Runqing Tan, Yi Wang, Yajun Jiang, Dingxin Ke, Han Du, Gang Guo, Kaiyun Liu","doi":"10.1016/j.micinf.2024.105433","DOIUrl":"10.1016/j.micinf.2024.105433","url":null,"abstract":"Helicobacter pylori (H. pylori) infection is a serious public health issue, and development of vaccines is a desirable preventive strategy for H. pylori. Toll-like receptor (TLR) ligands have shown potential as vaccine adjuvants that induce immune responses, but polyinosinic-polycytidylic acid (poly I:C), a nucleic acid-based TLR9 ligand, is less well studied in H. pylori vaccine research. Here, we evaluated the effects of poly I:C and CpG oligodeoxynucleotide (CpG ODN), a nucleic acid TLR3 ligand, as adjuvants in combination with the H. pylori recombinant proteins LpoB and UreA to protect against H. pylori infection. For analysis of specific immune responses, the levels of specific antibodies and splenic cytokines were measured in the immunized mice. Compared with CpG ODN, poly I:C could induce mucosal sIgA antibody responses and reduce H. pylori colonization. Additionally, the combination of poly I:C and CpG ODN caused greater immunoprotection and significantly reduced gastritis, exerting synergistic effects. Analysis of splenic cytokines revealed that poly I:C mainly triggered a mixed Th1/Th2/Th17 immune response, whereas the combination of CpG ODN and poly I:C induced a Th1/Th17 immune response. Our findings indicated that increased levels of mucosal sIgA antibodies and a robust splenic Th1/Th17 immune response were associated with reduced H. pylori colonization in vaccinated mice. This study identified a potential TLR ligand adjuvant for developing more effective H. pylori vaccines.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of anti-tuberculosis treatment on respiratory tract microbiome in pulmonary tuberculosis. 抗结核治疗对肺结核呼吸道微生物组的影响。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-10-19 DOI: 10.1016/j.micinf.2024.105432

Druti Hazra, Kiran Chawla, Fayaz S M, Vitali Sintchenko, Rahul Magazine, Elena Martinez, Akhilesh Pandey

{"title":"The impact of anti-tuberculosis treatment on respiratory tract microbiome in pulmonary tuberculosis.","authors":"Druti Hazra, Kiran Chawla, Fayaz S M, Vitali Sintchenko, Rahul Magazine, Elena Martinez, Akhilesh Pandey","doi":"10.1016/j.micinf.2024.105432","DOIUrl":"10.1016/j.micinf.2024.105432","url":null,"abstract":"The growing evidence has underscored the significance of interactions between the host and microbiota in respiratory health, presenting a novel perspective on disease management. Yet, comprehension of the respiratory microbiome shifts before and after anti-tuberculosis treatment is limited. This study compares respiratory microbiome profiles in untreated tuberculosis (UTB) and completed TB treatment (CTB) cases with healthy controls, using 16S rRNA sequencing on sputum samples. Significant reduction in sputum microbial alpha diversity was observed in both TB groups when compared to healthy controls (P < 0.05). Beta diversity analysis showed distinct clustering (P < 0.05). Linear discriminant analysis revealed an abundance of potentially pathogenic bacterial genera like Haemophilus, Pseudomonas, and Mycobacterium in the UTB group, while Streptococcus, Rothia, and Neisseria dominated in CTB samples. Healthy sputum microbiomes were enriched with Prevotella, Fusobacterium, Porphyromonadaceae_unclassified,andPeptostreptococcus. Moreover, predicted bacterial functional pathways showed significant differences among the three groups, mainly related to nutrient metabolism. These findings indicated significant microbial dysbiosis in sputum samples recovered from patients with pulmonary TB with an elevated presence of potentially pathogenic bacteria, depletion of beneficial genera, and downregulation of several essential metabolic pathways. Further exploration of respiratory microbiome-based diagnostic biomarkers and their role in targeted treatment strategies in tuberculosis is warranted.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HERV-W ENV transcription in B cells predicting symptomatic COVID-19 and risk for long COVID can express a full-length protein despite stop codon in mRNA from chromosome X via a ribosome readthrough. HERV-W ENV 在 B 细胞中的转录可预测有症状的 COVID-19 和长 COVID 风险，尽管来自 X 染色体的 mRNA 中存在终止密码子，但 HERV-W ENV 仍可通过核糖体通读表达全长蛋白质。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-10-16 DOI: 10.1016/j.micinf.2024.105431

Joanna Brunel, Julien Paganini, Melissa Galloux, Benjamin Charvet, Hervé Perron

{"title":"HERV-W ENV transcription in B cells predicting symptomatic COVID-19 and risk for long COVID can express a full-length protein despite stop codon in mRNA from chromosome X via a ribosome readthrough.","authors":"Joanna Brunel, Julien Paganini, Melissa Galloux, Benjamin Charvet, Hervé Perron","doi":"10.1016/j.micinf.2024.105431","DOIUrl":"10.1016/j.micinf.2024.105431","url":null,"abstract":"The human genome comprises 8 % of endogenous retroviruses (HERVs). Though HERVS contribute to physiological functions, copies retained pathogenic potential. The HERV-W ENV protein was shown expressed in patients with worse COVID-19 symptoms and post-COVID syndrome. A significant detection of the mRNA encoding HERV-W ENV from patients with COVID-19 in B cells from RNAseq reads obtained from peripheral blond mononuclear cells. This data stratified with increased COVID-19 symptoms or with post-acute sequelae of COVID-19 (long COVID) after 3 months. The HERV-W ENV-U3R RNA was confirmed to display the best alignment with chromosome X ERVWE2 locus. However, a stop codon precluding its translation was re-addressed after recent understandings of ribosome readthrough mechanisms. Experimental results evidenced that this HERV gene can effectively express a full-length protein in the presence of molecules allowing translation via a readthrough mechanism at the ribosome level. Results not only confirm HERV-W ENV RNA origin in these patients but show for the first time how a defective HERV copy can be translated into a complete protein when specific factors make it possible at the ribosome level. The present proof of concept now requires further studies to identify the factors involved in this newly understood mechanism, following SARS-CoV-2 exposure.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppressive effects of toll-like receptor 2, toll-like receptor 4, and toll-like receptor 7 on protective responses to Mycobacterium bovis BCG from epithelial cells. Toll-Like Receptor 2、Toll-Like Receptor 4 和 Toll-Like Receptor 7 对上皮细胞卡介苗分枝杆菌保护性反应的抑制作用。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-10-04 DOI: 10.1016/j.micinf.2024.105428

Aarti Singh, Akshita Singh, Shakuntala Surender Kumar Saraswati, Ankush Kumar Rana, Aayushi Singh, Chaitenya Verma, Vishal Sinha, Kanika Kalra, Krishnamurthy Natarajan

{"title":"Suppressive effects of toll-like receptor 2, toll-like receptor 4, and toll-like receptor 7 on protective responses to Mycobacterium bovis BCG from epithelial cells.","authors":"Aarti Singh, Akshita Singh, Shakuntala Surender Kumar Saraswati, Ankush Kumar Rana, Aayushi Singh, Chaitenya Verma, Vishal Sinha, Kanika Kalra, Krishnamurthy Natarajan","doi":"10.1016/j.micinf.2024.105428","DOIUrl":"10.1016/j.micinf.2024.105428","url":null,"abstract":"Mycobacteria have several mechanisms for evasion of protective responses mounted by the host. In this study, we unravel yet another mechanism that is mediated by Toll-Like Receptors TLR2, TLR4, and TLR7 in epithelial cells. We show that mycobacterial infection of epithelial cells increases the expression of TLR2, TLR4, and TLR7. Stimulation of either TLR along with mycobacterial infection results in an inhibition of oxidative burst resulting in increased survival of mycobacteria inside epithelial cells. TLR stimulation along with mycobacterial infection also inhibits activation of epithelial cells for T cell responses by differentially regulating the activation of ERK-MAPK and p38-MAPK along with inhibition of co-stimulatory molecule CD86 expression. Furthermore, stimulation of either TLR inhibits the induction of apoptosis and autophagy. Knockdown of either TLR by specific siRNAs reverses the inhibition by ROS and apoptosis by mycobacteria and results in reduced intracellular survival of mycobacteria in a MyD88-dependent manner. These results point towards a negative role for TLR2, TLR4, and TLR7 in regulating protective responses to M. bovis BCG infection in epithelial cells.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dopaminergic neuronal regulation determines innate immunity of Caenorhabditis elegans during Klebsiella aerogenes infection. 多巴胺能神经元调控决定了草履虫在产气克雷伯氏菌感染期间的先天免疫力。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-10-04 DOI: 10.1016/j.micinf.2024.105430

Thirumugam Gowripriya, Radhakrishnan Yashwanth, Prabhanand Bhaskar James, Ramamurthi Suresh, Krishnaswamy Balamurugan

{"title":"Dopaminergic neuronal regulation determines innate immunity of Caenorhabditis elegans during Klebsiella aerogenes infection.","authors":"Thirumugam Gowripriya, Radhakrishnan Yashwanth, Prabhanand Bhaskar James, Ramamurthi Suresh, Krishnaswamy Balamurugan","doi":"10.1016/j.micinf.2024.105430","DOIUrl":"10.1016/j.micinf.2024.105430","url":null,"abstract":"The innate immune signals are the front line of host defense against bacterial pathogens. Pathogen-induced harmful effects, such as reduced neuronal signals to the intestine, affect the host's food sensing and dwelling behavior. Here, we report that dopamine and kpc-1 signals control the intestinal innate immune responses through the p38/PMK-1 MAPK signaling pathway in C. elegans. K. aerogenes infection in C. elegans affects the food-dwelling behavior, which depends on dopamine regulation. The absence of the dopamine receptor (dop-1) and transporter (dat-1) increases attraction to the pathogen instead of avoidance. The K. aerogenes infection affects age-1 regulation through the furin-like proprotein convertase (kpc-1); the absence of kpc-1 affects environment-dependent dauer formation. In contrast, the dop-1 mutation antagonistically regulates intestinal immune regulation, while the kpc-1 mutation partially regulates the p38/PMK-1 MAPK pathway. Our findings indicate that dopamine and kpc-1signaling from the nervous system control intestinal immunity in an antagonistic and agonistic manner, respectively.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and in silico characterization of prophages in Helicobacter pylori clinical strains. 幽门螺旋杆菌临床菌株中噬菌体的筛选和硅学表征。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-10-03 DOI: 10.1016/j.micinf.2024.105429

Rute Ferreira, Graça Pinto, Eva Presa, Mónica Oleastro, Catarina Silva, Luís Vieira, Cláudia Sousa, Diana P Pires, Ceu Figueiredo, Luís D R Melo

{"title":"Screening and in silico characterization of prophages in Helicobacter pylori clinical strains.","authors":"Rute Ferreira, Graça Pinto, Eva Presa, Mónica Oleastro, Catarina Silva, Luís Vieira, Cláudia Sousa, Diana P Pires, Ceu Figueiredo, Luís D R Melo","doi":"10.1016/j.micinf.2024.105429","DOIUrl":"10.1016/j.micinf.2024.105429","url":null,"abstract":"The increase of antibiotic resistance calls for alternatives to control Helicobacter pylori, a Gram-negative bacterium associated with various gastric diseases. Bacteriophages (phages) can be highly effective in the treatment of pathogenic bacteria. Here, we developed a method to identify prophages in H. pylori genomes aiming at their future use in therapy. A polymerase chain reaction (PCR)-based technique tested five primer pairs on 74 clinical H. pylori strains. After the PCR screening, 14 strains most likely to carry prophages were fully sequenced. After that, a more holistic approach was taken by studying the complete genome of the strains. This study allowed us to identify 12 intact prophage sequences, which were then characterized concerning their morphology, virulence, and antibiotic-resistance genes. To understand the variability of prophages, a phylogenetic analysis using the sequences of all H. pylori phages reported to date was performed. Overall, we increased the efficiency of identifying complete prophages to 54.1 %. Genes with homology to potential virulence factors were identified in some new prophages. Phylogenetic analysis revealed a close relationship among H. pylori-phages, although there are phages with different geographical origins. This study provides a deeper understanding of H. pylori-phages, providing valuable insights into their potential use in therapy.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk between human endogenous retroviruses and exogenous viruses. 人类内源性逆转录病毒与外源性病毒之间的相互影响。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-09-28 DOI: 10.1016/j.micinf.2024.105427

Edoardo Pizzioli, Antonella Minutolo, Emanuela Balestrieri, Claudia Matteucci, Gkikas Magiorkinis, Branka Horvat

引用次数: 0

Microneedle-based arrays - Breakthrough strategy for the treatment of bacterial and fungal skin infections. 微针阵列--治疗细菌和真菌皮肤感染的突破性策略。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-09-24 DOI: 10.1016/j.micinf.2024.105426

Oliwia Kordyl, Zuzanna Styrna, Monika Wojtyłko, Bozena Michniak-Kohn, Tomasz Osmałek

{"title":"Microneedle-based arrays - Breakthrough strategy for the treatment of bacterial and fungal skin infections.","authors":"Oliwia Kordyl, Zuzanna Styrna, Monika Wojtyłko, Bozena Michniak-Kohn, Tomasz Osmałek","doi":"10.1016/j.micinf.2024.105426","DOIUrl":"https://doi.org/10.1016/j.micinf.2024.105426","url":null,"abstract":"Currently, fungal and bacterial skin infections rank among the most challenging public health problems due to the increasing prevalence of microorganisms and the development of resistance to available drugs. A major issue in treating these infections with conventional topical medications is the poor penetration through the stratum corneum, the outermost layer of the skin. The concept of microneedles seems to be a future-proof approach for delivering drugs directly into deeper tissues. By bypassing the skin barrier, microneedle systems allow therapeutic substances to reach deeper layers more efficiently, significantly improving treatment outcomes. Nonetheless, the primary challenges regarding the effectiveness of microneedles involve selecting the appropriate size and shape, along with polymer composition and fabrication technology, to enable controlled and efficient drug release. This review offers a comprehensive overview of the latest knowledge on microneedle types and manufacturing techniques, highlighting their potential effectiveness in treating bacterial and fungal skin infections. It includes updated statistics on infection prevalence and provides a detailed examination of common bacterial and fungal diseases, focusing on their symptoms, causative species, and treatment methods. Additionally, the review addresses safety considerations, regulatory aspects, and future perspectives for microneedle-based therapeutic systems. It also underscores the importance of industrialization and clinical translation efforts, emphasizing the significant potential of microneedle technology for advancing medical applications.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of various DNA and RNA viruses in bats in Yamaguchi Prefecture, Japan. 日本山口县蝙蝠体内各种 DNA 和 RNA 病毒的检测。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-09-24 DOI: 10.1016/j.micinf.2024.105425

Miyuka Nishizato, Urara Imai, Chisato Shigenaga, Miho Obata, Saki Mitsunaga, Marla Anggita, Samuel Nyampong, Shelly Wulandari, Weiyin Hu, Kazuki Kiuno, Lydia Mali Langata, Hiroyuki Imai, Masashi Sakurai, Tetsuya Yanagida, Ai Takano, Takashi Murakami, Chang-Gi Jeong, Jae-Ku Oem, Daisuke Hayasaka, Hiroshi Shimoda

{"title":"Detection of various DNA and RNA viruses in bats in Yamaguchi Prefecture, Japan.","authors":"Miyuka Nishizato, Urara Imai, Chisato Shigenaga, Miho Obata, Saki Mitsunaga, Marla Anggita, Samuel Nyampong, Shelly Wulandari, Weiyin Hu, Kazuki Kiuno, Lydia Mali Langata, Hiroyuki Imai, Masashi Sakurai, Tetsuya Yanagida, Ai Takano, Takashi Murakami, Chang-Gi Jeong, Jae-Ku Oem, Daisuke Hayasaka, Hiroshi Shimoda","doi":"10.1016/j.micinf.2024.105425","DOIUrl":"10.1016/j.micinf.2024.105425","url":null,"abstract":"Bats are important natural hosts of various zoonotic viruses, including Ebola virus, Lyssa virus, and severe acute respiratory syndrome coronavirus (SARS-CoV). Although investigation of bats is valuable for predicting emerging infectious diseases from these animals, few surveys of bat-derived viruses have been conducted in Japan. In the present study, samples were collected from a total of 132 bats of 4 different species from 4 different locations within Yamaguchi Prefecture; these sample were employed for comprehensive detection of bat-derived viruses by polymerase chain reaction (PCR) and reverse transcription (RT)-PCR using primers universal for each of 4 different viral classes. As a result of PCR and RT-PCR, various herpesviruses, astroviruses, coronaviruses, and adenoviruses were identified from a total of 80 bats. The detected herpesviruses belong to the Betaherpesvirinae or Gammaherpesvirinae subfamily, the detected adenoviruses to the genus Mastadenovirus, the detected astroviruses to the genus Mamastrovirus; and the detected coronaviruses belong to the genus Alphacoronavirus. The detected sequences of 12 strains of 4 families showed 100 % amino acid identity with viruses previously detected either in China or South Korea. These findings expand our understanding of viruses carried by bats, and provide insights into the nature of bat-derived viruses in Japan.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell transcriptome analysis of the early immune response in the lymph nodes of Borrelia burgdorferi-infected mice. 包柔氏菌感染小鼠淋巴结早期免疫反应的单细胞转录组分析。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-09-19 DOI: 10.1016/j.micinf.2024.105424

Varpu Rinne, Kirsi Gröndahl-Yli-Hannuksela, Ruth Fair-Mäkelä, Marko Salmi, Pia Rantakari, Tapio Lönnberg, Jukka Alinikula, Annukka Pietikäinen, Jukka Hytönen

{"title":"Single-cell transcriptome analysis of the early immune response in the lymph nodes of Borrelia burgdorferi-infected mice.","authors":"Varpu Rinne, Kirsi Gröndahl-Yli-Hannuksela, Ruth Fair-Mäkelä, Marko Salmi, Pia Rantakari, Tapio Lönnberg, Jukka Alinikula, Annukka Pietikäinen, Jukka Hytönen","doi":"10.1016/j.micinf.2024.105424","DOIUrl":"https://doi.org/10.1016/j.micinf.2024.105424","url":null,"abstract":"Lyme borreliosis is a disease caused by Borrelia burgdorferi sensu lato bacteria. Borrelia burgdorferi is known to induce prolonged extrafollicular immune responses and abnormal germinal centre formation. The infection fails to generate a neutralizing type of immunity, eventually establishing a persistent infection. Here, we performed single-cell RNA sequencing to characterize the immune landscape of lymph node lymphocytes during the early Borrelia burgdorferi infection in a murine model. Our results indicate key features of an extrafollicular immune response four days after Borrelia burgdorferi infection, including notable B cell proliferation, immunoglobulin class switching to IgG3 and IgG2b isotypes, plasmablast differentiation, and the presence of extrafollicular B cells identified through immunohistochemistry. Additionally, we found infection-derived upregulation of suppressor of cytokine signalling genes Socs1 and Socs3, along with downregulation of genes associated with MHC II antigen presentation in B cells. Our results support the central role of B cells in the immune response of a Borrelia burgdorferi infection, and provide cues of mechanisms behind the determination between extrafollicular and germinal centre responses during Borrelia burgdorferi infection.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0