Microbes and Infection最新文献_第2页

Photodynamic therapy reduces viability, enhances itraconazole activity, and impairs mitochondrial physiology of Sporothrix brasiliensis 光动力疗法降低了巴西孢子虫的活力，增强了伊曲康唑的活性，并损害了线粒体的生理机能。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105440

Mariana Lucy Mesquita Ramos , Azuil Barrinha , Glauber Ribeiro de Sousa Araújo , Vinicius Alves , Iara Bastos de Andrade , Dario Corrêa-Junior , Maria Cristina Machado Motta , Rodrigo Almeida-Paes , Susana Frases

{"title":"Photodynamic therapy reduces viability, enhances itraconazole activity, and impairs mitochondrial physiology of Sporothrix brasiliensis","authors":"Mariana Lucy Mesquita Ramos , Azuil Barrinha , Glauber Ribeiro de Sousa Araújo , Vinicius Alves , Iara Bastos de Andrade , Dario Corrêa-Junior , Maria Cristina Machado Motta , Rodrigo Almeida-Paes , Susana Frases","doi":"10.1016/j.micinf.2024.105440","DOIUrl":"10.1016/j.micinf.2024.105440","url":null,"abstract":"<div><div><em>Sporothrix brasiliensis</em> is the main agent of sporotrichosis in Brazil, with few therapeutic options. This study aimed to investigate the <em>in vitro</em> efficacy of photodynamic therapy using a diode laser (InGaAIP) in combination with the photosensitizer methylene blue against <em>S. brasiliensis</em> yeasts. Additionally, we evaluated the underexplored mitochondrial activity of <em>S. brasiliensis</em> and the impact of laser treatment on the fungal mitochondrial aspects post-treatment. Three strains of <em>S. brasiliensis</em> were used, including a non-wild-type strain to itraconazole. Yeast viability was determined by counting colony-forming units. For a comprehensive analysis of irradiated versus non-irradiated cells, we assessed combined therapy with itraconazole, scanning electron microscopy of cells, and mitochondrial activity. The latter included high-resolution respirometry, membrane potential analysis, and reactive oxygen species production. Methylene blue combined with photodynamic therapy inhibited the growth of the isolates, including the non-wild-type strain to itraconazole. Photodynamic therapy induced the production of reactive oxygen species, which negatively affected mitochondrial function, resulting in decreased membrane potential and cell death. Photodynamic therapy altered the ultrastructure and mitochondrial physiology of <em>S. brasiliensis</em>, suggesting a new therapeutic approach for sporotrichosis caused by this species.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105440"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of a monoclonal antibody targeting the SARS-CoV-2 Omicron spike protein and analysis of SARS-CoV-2 Omicron mutations related to monoclonal antibody resistance 生产针对 SARS-CoV-2 Omicron 穗蛋白的单克隆抗体，分析与单克隆抗体抗性有关的 SARS-CoV-2 Omicron 突变。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105461

Jinsoo Kim , Suyeon Kim , Sangkyu Park , Dongbum Kim , Minyoung Kim , Kyeongbin Baek , Bo Min Kang , Ha-Eun Shin , Myeong-Heon Lee , Younghee Lee , Hyung-Joo Kwon

{"title":"Production of a monoclonal antibody targeting the SARS-CoV-2 Omicron spike protein and analysis of SARS-CoV-2 Omicron mutations related to monoclonal antibody resistance","authors":"Jinsoo Kim , Suyeon Kim , Sangkyu Park , Dongbum Kim , Minyoung Kim , Kyeongbin Baek , Bo Min Kang , Ha-Eun Shin , Myeong-Heon Lee , Younghee Lee , Hyung-Joo Kwon","doi":"10.1016/j.micinf.2024.105461","DOIUrl":"10.1016/j.micinf.2024.105461","url":null,"abstract":"<div><div>SARS-CoV-2 mutations have resulted in the emergence of multiple concerning variants, with Omicron being the dominant strain presently. Therefore, we developed a monoclonal antibody (mAb) against the spike (S) protein of SARS-CoV-2 Omicron for therapeutic applications. We established the 1E3H12 mAb, recognizing the receptor binding domain (RBD) of the Omicron S protein, and found that the 1E3H12 mAb can efficiently recognize the Omicron S protein with weak affinity to the Alpha, Beta, and Mu variants, but not to the parental strain and Delta variant. Based on <em>in vitro</em> assays, the mAb demonstrated neutralizing activity against Omicron BA.1, BA.4/5, BQ.1.1, and XBB. A humanized antibody was further produced and proved to have neutralizing activity. To verify the potential limitations of the 1E3H12 mAb due to viral escape of SARS-CoV-2 Omicron variants, we analyzed the emergence of variants by whole genome deep sequencing after serial passage in cell culture. The results showed a few unique S protein mutations in the genome associated with resistance to the mAb. These findings suggest that this antibody not only contributes to the therapeutic arsenal against COVID-19 but also addresses the ongoing challenge of antibody resistance among the evolving subvariants of SARS-CoV-2 Omicron.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105461"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copyright page Elsevier 版权页面Elsevier

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/S1286-4579(25)00057-7

引用次数: 0

Intranasal immunization with poly I:C and CpG ODN adjuvants enhances the protective efficacy against Helicobacter pylori infection in mice 使用聚 I:C 和 CpG ODN 佐剂进行鼻内免疫可增强小鼠对幽门螺旋杆菌感染的保护效力。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105433

Min Sun, Yu Liu, Xiumei Ni, Runqing Tan, Yi Wang, Yajun Jiang, Dingxin Ke, Han Du, Gang Guo, Kaiyun Liu

{"title":"Intranasal immunization with poly I:C and CpG ODN adjuvants enhances the protective efficacy against Helicobacter pylori infection in mice","authors":"Min Sun, Yu Liu, Xiumei Ni, Runqing Tan, Yi Wang, Yajun Jiang, Dingxin Ke, Han Du, Gang Guo, Kaiyun Liu","doi":"10.1016/j.micinf.2024.105433","DOIUrl":"10.1016/j.micinf.2024.105433","url":null,"abstract":"<div><div><em>Helicobacter pylori</em> (<em>H. pylori</em>) infection is a serious public health issue, and development of vaccines is a desirable preventive strategy for <em>H. pylori</em>. Toll-like receptor (TLR) ligands have shown potential as vaccine adjuvants that induce immune responses, but polyinosinic-polycytidylic acid (poly I:C), a nucleic acid-based TLR9 ligand, is less well studied in <em>H. pylori</em> vaccine research. Here, we evaluated the effects of poly I:C and CpG oligodeoxynucleotide (CpG ODN), a nucleic acid TLR3 ligand, as adjuvants in combination with the <em>H. pylori</em> recombinant proteins LpoB and UreA to protect against <em>H. pylori</em> infection. For analysis of specific immune responses, the levels of specific antibodies and splenic cytokines were measured in the immunized mice. Compared with CpG ODN, poly I:C could induce mucosal sIgA antibody responses and reduce <em>H. pylori</em> colonization. Additionally, the combination of poly I:C and CpG ODN caused greater immunoprotection and significantly reduced gastritis, exerting synergistic effects. Analysis of splenic cytokines revealed that poly I:C mainly triggered a mixed Th1/Th2/Th17 immune response, whereas the combination of CpG ODN and poly I:C induced a Th1/Th17 immune response. Our findings indicated that increased levels of mucosal sIgA antibodies and a robust splenic Th1/Th17 immune response were associated with reduced <em>H. pylori</em> colonization in vaccinated mice. This study identified a potential TLR ligand adjuvant for developing more effective <em>H. pylori</em> vaccines.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105433"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miRNAs regulate the metabolic adaptation of Paracoccidioides brasiliensis during copper deprivation miRNA调控巴西副球孢子虫在铜匮乏期间的代谢适应。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105435

Dener Lucas Araújo dos Santos , Juliana Santana de Curcio , Evandro Novaes , Célia Maria de Almeida Soares

引用次数: 0

Schistosoma heamatobium tetraspanins TSP-2 and TSP-6 induce Dendritic Cells maturation, cytokine production and T helper cells differentiation in vitro 血吸虫四联蛋白 TSP-2 和 TSP-6 在体外诱导树突状细胞成熟、细胞因子产生和 T 辅助细胞分化。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105439

Angela Silvano , Javier Sotillo , Marta Cecchi , Alex Loukas , Mireille Ouedraogo , Astrid Parenti , Fabrizio Bruschi , Maria Gabriella Torcia , Valentina D Mangano

{"title":"Schistosoma heamatobium tetraspanins TSP-2 and TSP-6 induce Dendritic Cells maturation, cytokine production and T helper cells differentiation in vitro","authors":"Angela Silvano , Javier Sotillo , Marta Cecchi , Alex Loukas , Mireille Ouedraogo , Astrid Parenti , Fabrizio Bruschi , Maria Gabriella Torcia , Valentina D Mangano","doi":"10.1016/j.micinf.2024.105439","DOIUrl":"10.1016/j.micinf.2024.105439","url":null,"abstract":"<div><div>Urogenital schistosomiasis caused by <em>Schistosoma haematobium</em> is a major cause of disability in endemic areas. Despite its socio-economic burden, no vaccine exists and the parasite's immunobiology remains underexplored. Genome annotation has revealed over 40 different genes encoding tetraspanins, transmembrane proteins with known immunomodulatory properties in other plathelminthes. This study investigated the role of <em>Sh</em>-TSP-2, <em>Sh</em>-TSP-6 and <em>Sh</em>-TSP-23, which are expressed in the parasite's tegument and extracellular vesicles (EVs). Immature dendritic cells (DCs) from unexposed healthy donors were stimulated with these proteins to evaluate maturation maker expression and cytokine production. Also, pre-activated T CD4<sup>+</sup> cells were stimulated with the DCs supernatant to assess cytokine gene expression. <em>Sh</em>-TSP-2 and <em>Sh</em>-TSP-6 induced maturation markers and cytokine production in DCs: <em>Sh</em>-TSP-2 increased CD80 and CD83 levels and the concentration of both pro-inflammatory (IL-6, TNF) and regulatory (IL-10) cytokines, while <em>Sh</em>-TSP-6 increased the production of IL-6. Moreover, supernatants from <em>Sh</em>-TSP-2 stimulated DCs induced the expression of Th1 (IFNɣ) and regulatory (IL-10) cytokines in CD4<sup>+</sup> T cells, while <em>Sh-</em>TSP-6 induced Th2 (IL-4, IL-13) cytokine expression. These results provide evidence that <em>S. haematobium</em> tetraspanins modulate the response of human DCs and CD4<sup>+</sup> T cells <em>in vitro,</em> and support <em>Sh</em>-TSP-2 as a promising vaccine candidate.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105439"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of anti-tuberculosis treatment on respiratory tract microbiome in pulmonary tuberculosis 抗结核治疗对肺结核呼吸道微生物组的影响。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105432

Druti Hazra , Kiran Chawla , Fayaz S.M. , Vitali Sintchenko , Rahul Magazine , Elena Martinez , Akhilesh Pandey

{"title":"The impact of anti-tuberculosis treatment on respiratory tract microbiome in pulmonary tuberculosis","authors":"Druti Hazra , Kiran Chawla , Fayaz S.M. , Vitali Sintchenko , Rahul Magazine , Elena Martinez , Akhilesh Pandey","doi":"10.1016/j.micinf.2024.105432","DOIUrl":"10.1016/j.micinf.2024.105432","url":null,"abstract":"<div><div>The growing evidence has underscored the significance of interactions between the host and microbiota in respiratory health, presenting a novel perspective on disease management. Yet, comprehension of the respiratory microbiome shifts before and after anti-tuberculosis treatment is limited. This study compares respiratory microbiome profiles in untreated tuberculosis (UTB) and completed TB treatment (CTB) cases with healthy controls, using 16S rRNA sequencing on sputum samples. Significant reduction in sputum microbial alpha diversity was observed in both TB groups when compared to healthy controls (P < 0.05). Beta diversity analysis showed distinct clustering (P < 0.05). Linear discriminant analysis revealed an abundance of potentially pathogenic bacterial genera like <em>Haemophilus, Pseudomonas</em>, and <em>Mycobacterium</em> in the UTB group, while <em>Streptococcus, Rothia</em>, and <em>Neisseria</em> dominated in CTB samples. Healthy sputum microbiomes were enriched with <em>Prevotella, Fusobacterium, Porphyromonadaceae_unclassified,</em> <em>and</em> <em>Peptostreptococcus</em>. Moreover, predicted bacterial functional pathways showed significant differences among the three groups, mainly related to nutrient metabolism.</div><div>These findings indicated significant microbial dysbiosis in sputum samples recovered from patients with pulmonary TB with an elevated presence of potentially pathogenic bacteria, depletion of beneficial genera, and downregulation of several essential metabolic pathways. Further exploration of respiratory microbiome-based diagnostic biomarkers and their role in targeted treatment strategies in tuberculosis is warranted.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105432"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution and zoonotic risk of O1:K1 and O2:K1 avian pathogenic Escherichia coli 禽致病性O1:K1和O2:K1大肠杆菌的进化和人畜共患风险。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105462

Eun-Jin Ha , Seung-Min Hong , Kang-Seuk Choi , Hyuk-Joon Kwon

{"title":"Evolution and zoonotic risk of O1:K1 and O2:K1 avian pathogenic Escherichia coli","authors":"Eun-Jin Ha , Seung-Min Hong , Kang-Seuk Choi , Hyuk-Joon Kwon","doi":"10.1016/j.micinf.2024.105462","DOIUrl":"10.1016/j.micinf.2024.105462","url":null,"abstract":"<div><div>The O1 and O2 serogroups of avian pathogenic <em>E. coli</em> (APEC) and human extraintestinal pathogenic <em>E. coli</em> (huExPEC) are closely related, but their evolutionary relationships need to be further elucidated. This study classified nineteen O1 and O2 APEC into <em>rpoB</em> sequence types (RSTs) and compared them with reference huExPEC using molecular prophage typing, virulence and antibiotic resistance gene profiling, and comparative genomics. Most O1:K1 and O2:K1 APEC (73.7 %) were classified as RST46-1 and RST47-9. RST47-9 is unique to Korean O1 APEC and likely derives from RST46-1 APEC. The six APEC showed high genome coverage/identity with the Korean RST46-1 huExPEC. Based on RST network and comparative genomics, we hypothesized that the O1 antigen first appeared in RST19-1 and O2 in RST24-1 <em>E. coli</em> in humans. Then, O1 and O2-antigen horizontally transferred to human RST46-1, where a unique K1 capsule (K1-<em>cps</em>) first appeared. The Korean APEC and huExPEC share evolutionary CRISPR spacers but differ in molecular antibiograms and prophage contents. Thus, RST46-1 huExPEC transmitted and evolved in poultry. The zoonotic risks remain unknown, but the substantial virulence of the RST46-1 APEC indicates that the reverse zoonotic risk of huExPEC in poultry is alarming.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105462"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gingivitis- and periodontitis-associated microbiota in bovine deciduous incisor teeth – A preliminary study 牛乳牙门牙牙龈炎和牙周炎相关微生物群的初步研究。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105468

Juliana Vaccari , Ana C. Borsanelli , Flávia R.F. Athayde , Júlia R. Saraiva , Thamiris N.M. Ramos , Iveraldo S. Dutra

{"title":"Gingivitis- and periodontitis-associated microbiota in bovine deciduous incisor teeth – A preliminary study","authors":"Juliana Vaccari , Ana C. Borsanelli , Flávia R.F. Athayde , Júlia R. Saraiva , Thamiris N.M. Ramos , Iveraldo S. Dutra","doi":"10.1016/j.micinf.2024.105468","DOIUrl":"10.1016/j.micinf.2024.105468","url":null,"abstract":"<div><div>As ruminants are frequently affected by periodontal diseases, understanding their microbial communities is crucial. In this pilot study, we analyzed subgingival biofilm samples of young cattle across different states: clinically healthy (n = 5), gingivitis (n = 5), and periodontitis (n = 5) using 16S rRNA gene sequencing and co-occurrence network analysis. The findings revealed that Proteobacteria was the predominant phylum across all conditions, with Fusobacteriota constituting 27.6 % of the microbiota in periodontitis-affected sites. In healthy sites, <em>Moraxella</em> (21.11 %), <em>Neisseria</em> (13.16 %), and <em>Lautropia</em> (7.69 %) were the predominant genera; in gingivitis-affected sites, the leading genera were <em>Neisseria</em> (23.65 %), <em>Moraxella</em> (18.95 %), and <em>Conchiformibius</em> (10.79 %); and in periodontitis sites, <em>Caviibacter</em> (19.78 %), <em>Moraxella</em> (16.13 %), and <em>Fusobacterium</em> (7.56 %) were most prevalent. Richness and dissimilarity analyses did not show significant differences across the clinical states, but differences were found between gingivitis and periodontitis sites (p = 0.01) in diversity. The co-occurrence networks highlighted significant variances in the central phyla across the phenotypes, with a higher number of positive interactions observed in periodontitis-affected sites. Consequently, this study demonstrated that the microbiota associated with periodontitis in young cattle exhibits greater diversity compared to gingivitis. Notably, in the deciduous dentition of cattle, the genera <em>Caviibacter</em> and <em>Moraxella</em> are pivotal in the context of periodontitis and periodontal health, respectively.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105468"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and in silico characterization of prophages in Helicobacter pylori clinical strains 幽门螺旋杆菌临床菌株中噬菌体的筛选和硅学表征。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105429

Rute Ferreira , Graça Pinto , Eva Presa , Mónica Oleastro , Catarina Silva , Luís Vieira , Cláudia Sousa , Diana P. Pires , Ceu Figueiredo , Luís D.R. Melo

{"title":"Screening and in silico characterization of prophages in Helicobacter pylori clinical strains","authors":"Rute Ferreira , Graça Pinto , Eva Presa , Mónica Oleastro , Catarina Silva , Luís Vieira , Cláudia Sousa , Diana P. Pires , Ceu Figueiredo , Luís D.R. Melo","doi":"10.1016/j.micinf.2024.105429","DOIUrl":"10.1016/j.micinf.2024.105429","url":null,"abstract":"<div><div>The increase of antibiotic resistance calls for alternatives to control <em>Helicobacter pylori</em>, a Gram-negative bacterium associated with various gastric diseases. Bacteriophages (phages) can be highly effective in the treatment of pathogenic bacteria. Here, we developed a method to identify prophages in <em>H. pylori</em> genomes aiming at their future use in therapy. A polymerase chain reaction (PCR)-based technique tested five primer pairs on 74 clinical <em>H. pylori</em> strains. After the PCR screening, 14 strains most likely to carry prophages were fully sequenced. After that, a more holistic approach was taken by studying the complete genome of the strains. This study allowed us to identify 12 intact prophage sequences, which were then characterized concerning their morphology, virulence, and antibiotic-resistance genes. To understand the variability of prophages, a phylogenetic analysis using the sequences of all <em>H. pylori</em> phages reported to date was performed. Overall, we increased the efficiency of identifying complete prophages to 54.1 %. Genes with homology to potential virulence factors were identified in some new prophages. Phylogenetic analysis revealed a close relationship among <em>H. pylori</em>-phages, although there are phages with different geographical origins. This study provides a deeper understanding of <em>H. pylori</em>-phages, providing valuable insights into their potential use in therapy.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105429"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0