Microbes and Infection最新文献_第2页

Averting collapse: Reimagining the NTDs ecosystem through G20 health diplomacy and science innovation. 避免崩溃：通过G20卫生外交和科学创新重塑被忽视热带病生态系统。

IF 2.7 4区医学

Microbes and Infection Pub Date : 2025-07-19 DOI: 10.1016/j.micinf.2025.105547

Maple Goh, Peter J Hotez

引用次数: 0

Therapeutic strategies to combat Staphylococcus aureus infections in cystic fibrosis. 抗囊性纤维化金黄色葡萄球菌感染的治疗策略。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-07-14 DOI: 10.1016/j.micinf.2025.105546

Aygun Israyilova, Gabriele Trespidi, Viola Camilla Scoffone, Silvia Buroni

引用次数: 0

Neutrophils display antibacterial defense via non-canonical LC3 decoration of extracellular bacteria. 中性粒细胞通过胞外细菌的非规范LC3装饰显示抗菌防御。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-07-04 DOI: 10.1016/j.micinf.2025.105545

Jurate Skerniskyte, Marina Valente Barroso, Johana Chicher, Philippe Hammann, Valerie Demais, Kathryn Wright, Serge Mostowy, Benoit S Marteyn

{"title":"Neutrophils display antibacterial defense via non-canonical LC3 decoration of extracellular bacteria.","authors":"Jurate Skerniskyte, Marina Valente Barroso, Johana Chicher, Philippe Hammann, Valerie Demais, Kathryn Wright, Serge Mostowy, Benoit S Marteyn","doi":"10.1016/j.micinf.2025.105545","DOIUrl":"10.1016/j.micinf.2025.105545","url":null,"abstract":"<p><p>Neutrophils play a pivotal role in the innate immune response to bacterial infection, being one of the first immune cells to reach infectious sites. Bacterial infection may induce neutrophil degranulation, production of neutrophil extracellular traps (NETs), or pathogen phagocytosis. While LC3 is typically linked to autophagy, here we observed a non-canonical role of LC3 when peripheral neutrophils interact with bacteria both in vivo and in vitro, using Shigella spp. as a model. Upon incubation with neutrophils, extracellular bacteria became labelled by LC3 (LC3+) along with granules-localised antimicrobial components, such as lactotransferrin, defensin, elastase, and myeloperoxidase, as demonstrated by mass spectrometry. Co-localisation of LC3 and plasma membrane-specific dyes indicated that neutrophil plasma membrane-derived elongated structures covering bacteria were responsible for the labelling. This phenomenon was associated with bacterial growth restriction and bacterial cell-death induction. Testing with specific inhibitors demonstrated that this labelling was dependent on functional V-type ATP synthase. Covering bacteria with membrane-derived elongated structures enhanced the subsequent phagocytosis of bacteria by neutrophils. Finally, the LC3 labelling rate increased with higher bacterial burden. In conclusion, we propose that this defense mechanism is beneficial when the burden of bacterial infection overwhelms neutrophils' capacity for phagocytosis.</p>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105545"},"PeriodicalIF":2.6,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transactivation of human endogenous retroviruses is associated with HIV-1 reservoir, lymphocyte activation and low CD4 count in virologically suppressed PLWH 在病毒学抑制的PLWH中，人内源性逆转录病毒的反激活与HIV-1库、淋巴细胞激活和低CD4计数有关。

IF 2.7 4区医学

Microbes and Infection Pub Date : 2025-07-01 DOI: 10.1016/j.micinf.2025.105478

Vita Petrone , Rossana Scutari , Vincenzo Malagnino , Lorenzo Piermatteo , Mirko Compagno , Romina Salpini , Martina Giudice , Marialaura Fanelli , Elisabetta Teti , Marco Iannetta , Antonella Minutolo , Maria Mercedes Santoro , Valentina Svicher , Paola Sinibaldi Vallebona , Massimo Andreoni , Emanuela Balestrieri , Loredana Sarmati , Francesca Ceccherini-Silberstein , Sandro Grelli , Claudia Matteucci

{"title":"The transactivation of human endogenous retroviruses is associated with HIV-1 reservoir, lymphocyte activation and low CD4 count in virologically suppressed PLWH","authors":"Vita Petrone , Rossana Scutari , Vincenzo Malagnino , Lorenzo Piermatteo , Mirko Compagno , Romina Salpini , Martina Giudice , Marialaura Fanelli , Elisabetta Teti , Marco Iannetta , Antonella Minutolo , Maria Mercedes Santoro , Valentina Svicher , Paola Sinibaldi Vallebona , Massimo Andreoni , Emanuela Balestrieri , Loredana Sarmati , Francesca Ceccherini-Silberstein , Sandro Grelli , Claudia Matteucci","doi":"10.1016/j.micinf.2025.105478","DOIUrl":"10.1016/j.micinf.2025.105478","url":null,"abstract":"<div><div>In the context of long-term therapy in virologically suppressed people living with HIV-1 (PLWH), the identification of new biomarkers associated with immuno-virological discordance, and the risk of disease progression is needed. Herein we investigated HERVs expression in association with immuno-virological discordance parameters for the identification of novel markers for the clinical monitoring of virologically suppressed PLWH. It is known the human endogenous retroviruses (HERVs), relics of ancestral exogenous retroviral infections comprising 8 % of human genome, could be reactivated by exogenous viruses including HIV-1. The study included 31 virologically suppressed PLWH and 10 healthy donors; blood HIV-DNA levels and residual plasma viremia were quantified by droplet digital-PCR, the expression of HERVs by RT-Real time PCR, and immunophenotyping by flow cytometry. The results revealed a dynamic association of HERVs with several virological and immunological parameters such as the HIV-1 reservoir, CD4 cell count, CD4 nadir and with CD8 and CD19 lymphocyte activation. In an era of searching innovative biomarkers for people living with HIV-1, the interconnection of HERVs with the HIV-1 reservoir and lymphocyte activation opens to further investigation on HERVs role in persistent immune activation in virologically suppressed PLWH, proposing them as potential new markers for clinical monitoring.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 5","pages":"Article 105478"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A tale of a hidden family of genetic immigrants 一个隐秘的基因移民家庭的故事。

IF 2.7 4区医学

Microbes and Infection Pub Date : 2025-07-01 DOI: 10.1016/j.micinf.2024.105387

Hervé Perron

引用次数: 0

HERV reactivation by adenovirus infection is associated with viral immune regulation "腺病毒感染导致的 HERV 再激活与病毒免疫调节有关"。

IF 2.7 4区医学

Microbes and Infection Pub Date : 2025-07-01 DOI: 10.1016/j.micinf.2024.105466

Wen Liang , Miona Stubbe , Lisa Pleninger , Anna Hofferek , Hans Stubbe , Julia Mai , Salih Özer , Dmitrij Frishman , Sabrina Schreiner , Michelle Vincendeau

{"title":"HERV reactivation by adenovirus infection is associated with viral immune regulation","authors":"Wen Liang , Miona Stubbe , Lisa Pleninger , Anna Hofferek , Hans Stubbe , Julia Mai , Salih Özer , Dmitrij Frishman , Sabrina Schreiner , Michelle Vincendeau","doi":"10.1016/j.micinf.2024.105466","DOIUrl":"10.1016/j.micinf.2024.105466","url":null,"abstract":"<div><div>Human endogenous retroviruses (HERVs), which are normally silenced by methylation or mutation, can be reactivated by a variety of environmental factors, including infection with exogenous viruses. In this work, we investigated the transcriptional activity of HERVs following infection of human liver cells (HepaRG) with human adenovirus C serotype 5 (HAdV-C5). HAdV-C5 infection results in reactivation of several HERV groups as well as differentially expressed genes. Interestingly, in HAdV-C5 infection, upregulated genes that were in close chromosomal proximity to upregulated HERV loci were associated with influencing viral carcinogenesis and inflammatory signaling. We also identified an FBXO17 transcript encoding an intronic ERVK9-11 sense sequence upon HAdV-C5 infection. FBXO17 has previously been described as an important factor in the regulation of the interferon response. This suggests that specific HERV groups may have the potential to trigger gene networks and influence viral immune responses.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 5","pages":"Article 105466"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular dissection of HERV-W dependent microglial- and astroglial cell polarization 依赖于 HERV-W 的小胶质细胞和星形胶质细胞极化的分子剖析。

IF 2.7 4区医学

Microbes and Infection Pub Date : 2025-07-01 DOI: 10.1016/j.micinf.2024.105382

Joel Gruchot , Laura Reiche , Luisa Werner , Felisa Herrero , Jessica Schira-Heinen , Urs Meyer , Patrick Küry

{"title":"Molecular dissection of HERV-W dependent microglial- and astroglial cell polarization","authors":"Joel Gruchot , Laura Reiche , Luisa Werner , Felisa Herrero , Jessica Schira-Heinen , Urs Meyer , Patrick Küry","doi":"10.1016/j.micinf.2024.105382","DOIUrl":"10.1016/j.micinf.2024.105382","url":null,"abstract":"<div><div>The endogenous retrovirus type W (HERV-W) is a human-specific entity, which was initially discovered in multiple sclerosis (MS) patient derived cells. We initially found that the HERV-W envelope (ENV) protein negatively affects oligodendrogenesis and controls microglial cell polarization towards a myelinated axon associated and damaging phenotype. Such first functional assessments were conducted <em>ex vivo</em>, given the human-specific origin of HERV-W. Recent experimental evidence gathered on a novel transgenic mouse model, mimicking activation and expression of the HERV-W ENV protein, revealed that all glial cell types are impacted and that cellular fates, differentiation, and functions were changed. In order to identify HERV-W-specific signatures in glial cells, the current study analyzed the transcriptome of ENV protein stimulated microglial- and astroglial cells and compared the transcriptomic signatures to lipopolysaccharide (LPS) stimulated cells, owing to the fact that both ligands can activate toll-like receptor-4 (TLR-4). Additionally, a comparison between published disease associated glial signatures and the transcriptome of HERV-W ENV stimulated glial cells was conducted. We, therefore, provide here for the first time a detailed molecular description of specific HERV-W ENV evoked effects on those glial cell populations that are involved in smoldering neuroinflammatory processes relevant for progression of neurodegenerative diseases.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 5","pages":"Article 105382"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HERV-W envelope protein is present in microglial cells of the human glioma tumor microenvironment and differentially modulates neoplastic cell behavior HERV-W 包膜蛋白存在于人类胶质瘤肿瘤微环境的小胶质细胞中，并对肿瘤细胞的行为起着不同的调节作用。

IF 2.7 4区医学

Microbes and Infection Pub Date : 2025-07-01 DOI: 10.1016/j.micinf.2024.105460

Laura Reiche , Benedikt Plaack , Maike Lehmkuhl , Vivien Weyers , Joel Gruchot , Daniel Picard , Hervé Perron , Marc Remke , Christiane Knobbe-Thomsen , Guido Reifenberger , Patrick Küry , David Kremer

{"title":"HERV-W envelope protein is present in microglial cells of the human glioma tumor microenvironment and differentially modulates neoplastic cell behavior","authors":"Laura Reiche , Benedikt Plaack , Maike Lehmkuhl , Vivien Weyers , Joel Gruchot , Daniel Picard , Hervé Perron , Marc Remke , Christiane Knobbe-Thomsen , Guido Reifenberger , Patrick Küry , David Kremer","doi":"10.1016/j.micinf.2024.105460","DOIUrl":"10.1016/j.micinf.2024.105460","url":null,"abstract":"<div><div>Gliomas are the most common parenchymal tumors of the central nervous system (CNS). With regard to their still unclear etiology, several recent studies have provided evidence of a new category of pathogenic elements called human endogenous retroviruses (HERVs) which seem to contribute to the evolution and progression of many neurological diseases such as amyotrophic lateral sclerosis (ALS), schizophrenia, chronic inflammatory polyneuropathy (CIDP) and, particularly, multiple sclerosis (MS). In these diseases, HERVs exert effects on cellular processes such as inflammation, proliferation, and migration. In previous studies, we demonstrated that in MS, the human endogenous retrovirus type-W envelope protein (HERV-W ENV) interferes with lesion repair through the activation of microglia (MG), the innate myeloid immune cells of the CNS. Here, we now show that HERV-W ENV is also present in the microglial cells (MG) of the tumor microenvironment (TME) in gliomas. It modulates the behavior of glioblastoma (GBM) cell lines in GBM/MG cocultures by altering their gene expression, secreted cytokines, morphology, proliferation, and migration properties and could thereby contribute to key tumor properties.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 5","pages":"Article 105460"},"PeriodicalIF":2.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk between human endogenous retroviruses and exogenous viruses 人类内源性逆转录病毒与外源性病毒之间的相互影响。

IF 2.7 4区医学

Microbes and Infection Pub Date : 2025-07-01 DOI: 10.1016/j.micinf.2024.105427

Edoardo Pizzioli , Antonella Minutolo , Emanuela Balestrieri , Claudia Matteucci , Gkikas Magiorkinis , Branka Horvat

引用次数: 0

Copyright page Elsevier 版权页面Elsevier

IF 2.7 4区医学

Microbes and Infection Pub Date : 2025-07-01 DOI: 10.1016/S1286-4579(25)00085-1

引用次数: 0