Microbes and Infection最新文献_第2页

Photodynamic therapy reduces viability, enhances itraconazole activity, and impairs mitochondrial physiology of Sporothrix brasiliensis. 光动力疗法降低了巴西孢子虫的活力，增强了伊曲康唑的活性，并损害了线粒体的生理机能。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-17 DOI: 10.1016/j.micinf.2024.105440

Mariana Lucy Mesquita Ramos, Azuil Barrinha, Glauber Ribeiro de Sousa Araújo, Vinicius Alves, Iara Bastos de Andrade, Dario Corrêa-Junior, Maria Cristina Machado Motta, Rodrigo Almeida-Paes, Susana Frases

{"title":"Photodynamic therapy reduces viability, enhances itraconazole activity, and impairs mitochondrial physiology of Sporothrix brasiliensis.","authors":"Mariana Lucy Mesquita Ramos, Azuil Barrinha, Glauber Ribeiro de Sousa Araújo, Vinicius Alves, Iara Bastos de Andrade, Dario Corrêa-Junior, Maria Cristina Machado Motta, Rodrigo Almeida-Paes, Susana Frases","doi":"10.1016/j.micinf.2024.105440","DOIUrl":"10.1016/j.micinf.2024.105440","url":null,"abstract":"<p><p>Sporothrix brasiliensis is the main agent of sporotrichosis in Brazil, with few therapeutic options. This study aimed to investigate the in vitro efficacy of photodynamic therapy using a diode laser (InGaAIP) in combination with the photosensitizer methylene blue against S. brasiliensis yeasts. Additionally, we evaluated the underexplored mitochondrial activity of S. brasiliensis and the impact of laser treatment on the fungal mitochondrial aspects post-treatment. Three strains of S. brasiliensis were used, including a non-wild-type strain to itraconazole. Yeast viability was determined by counting colony-forming units. For a comprehensive analysis of irradiated versus non-irradiated cells, we assessed combined therapy with itraconazole, scanning electron microscopy of cells, and mitochondrial activity. The latter included high-resolution respirometry, membrane potential analysis, and reactive oxygen species production. Methylene blue combined with photodynamic therapy inhibited the growth of the isolates, including the non-wild-type strain to itraconazole. Photodynamic therapy induced the production of reactive oxygen species, which negatively affected mitochondrial function, resulting in decreased membrane potential and cell death. Photodynamic therapy altered the ultrastructure and mitochondrial physiology of S. brasiliensis, suggesting a new therapeutic approach for sporotrichosis caused by this species.</p>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105440"},"PeriodicalIF":2.6,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Schistosoma heamatobium tetraspanins TSP-2 and TSP-6 induce Dendritic Cells maturation, cytokine production and T helper cells differentiation in vitro. 血吸虫四联蛋白 TSP-2 和 TSP-6 在体外诱导树突状细胞成熟、细胞因子产生和 T 辅助细胞分化。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-15 DOI: 10.1016/j.micinf.2024.105439

Angela Silvano, Javier Sotillo, Marta Cecchi, Alex Loukas, Mireille Ouedraogo, Astrid Parenti, Fabrizio Bruschi, Maria Gabriella Torcia, Valentina D Mangano

{"title":"Schistosoma heamatobium tetraspanins TSP-2 and TSP-6 induce Dendritic Cells maturation, cytokine production and T helper cells differentiation in vitro.","authors":"Angela Silvano, Javier Sotillo, Marta Cecchi, Alex Loukas, Mireille Ouedraogo, Astrid Parenti, Fabrizio Bruschi, Maria Gabriella Torcia, Valentina D Mangano","doi":"10.1016/j.micinf.2024.105439","DOIUrl":"10.1016/j.micinf.2024.105439","url":null,"abstract":"<p><p>Urogenital schistosomiasis caused by Schistosoma haematobium is a major cause of disability in endemic areas. Despite its socio-economic burden, no vaccine exists and the parasite's immunobiology remains underexplored. Genome annotation has revealed over 40 different genes encoding tetraspanins, transmembrane proteins with known immunomodulatory properties in other plathelminthes. This study investigated the role of Sh-TSP-2, Sh-TSP-6 and Sh-TSP-23, which are expressed in the parasite's tegument and extracellular vesicles (EVs). Immature dendritic cells (DCs) from unexposed healthy donors were stimulated with these proteins to evaluate maturation maker expression and cytokine production. Also, pre-activated T CD4<sup>+</sup> cells were stimulated with the DCs supernatant to assess cytokine gene expression. Sh-TSP-2 and Sh-TSP-6 induced maturation markers and cytokine production in DCs: Sh-TSP-2 increased CD80 and CD83 levels and the concentration of both pro-inflammatory (IL-6, TNF) and regulatory (IL-10) cytokines, while Sh-TSP-6 increased the production of IL-6. Moreover, supernatants from Sh-TSP-2 stimulated DCs induced the expression of Th1 (IFNɣ) and regulatory (IL-10) cytokines in CD4<sup>+</sup> T cells, while Sh-TSP-6 induced Th2 (IL-4, IL-13) cytokine expression. These results provide evidence that S. haematobium tetraspanins modulate the response of human DCs and CD4<sup>+</sup> T cells in vitro, and support Sh-TSP-2 as a promising vaccine candidate.</p>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105439"},"PeriodicalIF":2.6,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chytridiomycosis disrupts metabolic responses in amphibians at metamorphic climax. 恙虫病扰乱了处于变态高潮期的两栖动物的新陈代谢反应。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-15 DOI: 10.1016/j.micinf.2024.105438

Josephine E Humphries, Steven D Melvin, Chantal Lanctôt, Hamish McCallum, David Newell, Laura F Grogan

{"title":"Chytridiomycosis disrupts metabolic responses in amphibians at metamorphic climax.","authors":"Josephine E Humphries, Steven D Melvin, Chantal Lanctôt, Hamish McCallum, David Newell, Laura F Grogan","doi":"10.1016/j.micinf.2024.105438","DOIUrl":"10.1016/j.micinf.2024.105438","url":null,"abstract":"<p><p>The fungal disease chytridiomycosis (causative agent Batrachochytrium dendrobatidis [Bd]) is a primary contributor to amphibian species declines. The morphological and physiological reorganization that occurs during amphibian metamorphosis likely increases the vulnerability of metamorphs to Bd. To address this, we exposed pro-metamorphic tadpoles of Fleay's barred frog (Mixophyes fleayi) to Bd and sampled skin and liver sections from control and exposed animals throughout metamorphosis (Gosner stages 40, 42 and 45). We used an untargeted metabolomics approach to assess the metabolic impacts of Bd infection during the critical metamorphic stages, extracting metabolites from sampled tissues and analysing them via Nuclear Magnetic Resonance spectrometry. Most exposed animals became moribund at Gosner stage 45, while a subset seemingly cleared their infections. Metabolite abundance varied throughout development, with Gosner stage 45 samples distinct from previous stages. Clinically infected animals at Gosner stage 45 exhibited profound metabolic dysregulation (e.g., upregulation of amino acid biosynthesis and degradation) in comparison to uninfected groups (negative controls and 'cleared' animals). Despite showing parallels with previous metabolomic analyses of Bd-infected adult frogs, we identified variations in our results that could be attributed to the dramatic changes that characterise metamorphosis and may be driving the heightened vulnerability observed in metamorphic amphibians.</p>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105438"},"PeriodicalIF":2.6,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nano-enhanced benzylpenicillin: Bridging antibacterial action with anti-inflammatory potential against antibiotic-resistant bacteria. 纳米增强型苄青霉素：抗生素耐药细菌的抗菌作用与消炎潜力的桥梁

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-13 DOI: 10.1016/j.micinf.2024.105436

Natália Cristina Gomes-da-Silva, Álefe Roger Silva França, Clenilton Costa Dos Santos, Luciana Magalhães Rebelo Alencar, Elaine Cruz Rosas, Luana Barbosa Corrêa, Carolline M A Lorentino, André L S Santos, Eduardo Ricci-Junior, Ralph Santos-Oliveira

{"title":"Nano-enhanced benzylpenicillin: Bridging antibacterial action with anti-inflammatory potential against antibiotic-resistant bacteria.","authors":"Natália Cristina Gomes-da-Silva, Álefe Roger Silva França, Clenilton Costa Dos Santos, Luciana Magalhães Rebelo Alencar, Elaine Cruz Rosas, Luana Barbosa Corrêa, Carolline M A Lorentino, André L S Santos, Eduardo Ricci-Junior, Ralph Santos-Oliveira","doi":"10.1016/j.micinf.2024.105436","DOIUrl":"10.1016/j.micinf.2024.105436","url":null,"abstract":"<p><p>This study investigates the enhancement of benzylpenicillin's antibacterial properties using nanomedicine, specifically by developing benzylpenicillin nanoemulsions. To address the escalating issue of bacterial resistance, we employed the advanced techniques Raman spectroscopy and atomic force microscopy to analyze the nanoemulsions' molecular structure and characteristics. We then evaluated the impact of these nanoemulsions on nitric oxide production by macrophages to deternine their potential to modulate inflammatory responses. We further assessed the antibacterial effectiveness of the nanoparticles against the pathogens Streptococcus pyogenes (Group A Streptococcus) and Streptococcus agalactiae (Group B Streptococcus). The results of antibiograms showed significant efficacy against Gram-positive bacteria, with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values, confirming their bactericidal potential. The investigation into the mechanism of action suggested substantial disruption to bacterial membrane integrity, underscoring a possible mode of antibacterial activity. Overall, the study provides valuable insights into the synergistic relationship between antibiotics and nanoparticles. In particular, it demonstrates the potential of benzylpenicillin nanoparticles to enhance the antimicrobial efficacy and influence inflammatory responses obtained by evaluating nitrite, IL-6 and TNF-α, offering promising avenues for future clinical applications and strategies to combat bacterial resistance.</p>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105436"},"PeriodicalIF":2.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TGEV nonstructural protein ORF3b upregulates the expression of SLA-DR at the transcriptional level in monocyte-derived porcine dendritic cells. TGEV非结构蛋白ORF3b可在转录水平上上调单核细胞衍生猪树突状细胞中SLA-DR的表达。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-13 DOI: 10.1016/j.micinf.2024.105437

Hang Liu, Mengyao Ma, Xinhao Jia, Mengwei Qian, Bo Pang, Muzi Li, Honglei Zhang, Shijie Ma, Lanlan Zheng

{"title":"TGEV nonstructural protein ORF3b upregulates the expression of SLA-DR at the transcriptional level in monocyte-derived porcine dendritic cells.","authors":"Hang Liu, Mengyao Ma, Xinhao Jia, Mengwei Qian, Bo Pang, Muzi Li, Honglei Zhang, Shijie Ma, Lanlan Zheng","doi":"10.1016/j.micinf.2024.105437","DOIUrl":"10.1016/j.micinf.2024.105437","url":null,"abstract":"<p><p>Transmissible gastroenteritis virus (TGEV) is a porcine intestinal pathogenic coronavirus that can cause acute intestinal diseases in pigs, especially in suckling piglets under two weeks of age, with a mortality rate of 100 %. Dendritic cells (DCs) are important antigen-presenting cells (APCs) that are essential for the initiation and modulation of immune responses in animals. In this study, we used monocyte-derived porcine DCs as an in vitro model of APCs to further study the pathogenic mechanism of TGEV. Our results demonstrated that TGEV successfully replicates in monocyte-derived porcine DCs, whereas UV-inactivated TGEV failed to infect these cells. Importantly, TGEV infection of DCs led to significant upregulation of swine leukocyte antigen II DR (SLA-DR), a key molecule in the major histocompatibility complex class II (MHC-II) family. We further demonstrated that the ORF3b nonstructural protein of TGEV significantly enhances SLA-DR expression at the transcriptional level in porcine DCs. This study provides new insights into the pathogenic mechanisms of TGEV.</p>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105437"},"PeriodicalIF":2.6,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miRNAs regulate the metabolic adaptation of Paracoccidioides brasiliensis during copper deprivation. miRNA调控巴西副球孢子虫在铜匮乏期间的代谢适应。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-09 DOI: 10.1016/j.micinf.2024.105435

Dener Lucas Araújo Dos Santos, Juliana Santana de Curcio, Evandro Novaes, Célia Maria de Almeida Soares

引用次数: 0

Bad company? The pericardium microbiome in people investigated for tuberculous pericarditis in an HIV-prevalent setting. 坏伙伴？在艾滋病流行的环境中，接受结核性心包炎调查者的心包微生物组。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-09 DOI: 10.1016/j.micinf.2024.105434

Georgina Nyawo, Charissa C Naidoo, Benjamin G Wu, Benjamin Kwok, Jose C Clemente, Yonghua Li, Stephanie Minnies, Byron Reeve, Suventha Moodley, Thadathilankal-Jess John, Sumanth Karamchand, Shivani Singh, Alfonso Pecararo, Anton Doubell, Charles Kyriakakis, Robin Warren, Leopoldo N Segal, Grant Theron

{"title":"Bad company? The pericardium microbiome in people investigated for tuberculous pericarditis in an HIV-prevalent setting.","authors":"Georgina Nyawo, Charissa C Naidoo, Benjamin G Wu, Benjamin Kwok, Jose C Clemente, Yonghua Li, Stephanie Minnies, Byron Reeve, Suventha Moodley, Thadathilankal-Jess John, Sumanth Karamchand, Shivani Singh, Alfonso Pecararo, Anton Doubell, Charles Kyriakakis, Robin Warren, Leopoldo N Segal, Grant Theron","doi":"10.1016/j.micinf.2024.105434","DOIUrl":"10.1016/j.micinf.2024.105434","url":null,"abstract":"<p><strong>Background: </strong>The site-of-disease microbiome and predicted metagenome were evaluated in a cross-sectional study involving people with presumptive tuberculous pericarditis. We also explored the interaction between C-reactive protein (CRP) and the microbiome.</p><p><strong>Methods: </strong>People with effusions requiring diagnostic pericardiocentesis (n=139) provided pericardial fluid for sequencing and blood for CRP measurement.</p><p><strong>Results: </strong>Pericardial fluid microbiota differed in β-diversity among people with definite (dTB, n=91), probable (pTB, n=25), and non- (nTB, n=23) tuberculous pericarditis. dTBs were Mycobacterium-, Lacticigenium-, and Kocuria-enriched vs. nTBs. HIV-positive dTBs were Mycobacterium-, Bifidobacterium-, Methylobacterium-, and Leptothrix-enriched vs. HIV-negative dTBs. HIV-positive dTBs on ART were Mycobacterium- and Bifidobacterium-depleted vs. those not on ART. dTBs exhibited enrichment in short-chain fatty acid (SCFA) and mycobacterial metabolism pathways vs. nTBs. Additional non-pericardial involvement (pulmonary infiltrates) was associated with Mycobacterium-enrichment and Streptococcus-depletion. Mycobacterium reads were in 34 % (31/91) of dTBs, 8 % (2/25) of pTBs and 17 % (4/23) nTBs. People with CRP above (vs. below) the median value had different β-diversity (Pseudomonas-depleted). No correlation was found between enriched taxa in dTBs and CRP.</p><p><strong>Conclusions: </strong>Pericardial fluid microbial composition varies by tuberculosis status, HIV (and ART) status and dTBs are enriched in SCFA-associated taxa. The clinical significance, including mycobacterial reads in nTBs and pTBs, requires evaluation.</p>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105434"},"PeriodicalIF":2.6,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unraveling the role of human microglia in tick-borne encephalitis virus infection: insights into neuroinflammation and viral pathogenesis 揭示人类小胶质细胞在蜱传脑炎病毒感染中的作用：洞察神经炎症和病毒发病机理。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105383

Veronika Pranclova , Lenka Nedvedova , Eliska Kotounova , Vaclav Hönig , Marketa Dvorakova , Marika Davidkova , Tomas Bily , Marie Vancova , Daniel Ruzek , Martin Palus

{"title":"Unraveling the role of human microglia in tick-borne encephalitis virus infection: insights into neuroinflammation and viral pathogenesis","authors":"Veronika Pranclova , Lenka Nedvedova , Eliska Kotounova , Vaclav Hönig , Marketa Dvorakova , Marika Davidkova , Tomas Bily , Marie Vancova , Daniel Ruzek , Martin Palus","doi":"10.1016/j.micinf.2024.105383","DOIUrl":"10.1016/j.micinf.2024.105383","url":null,"abstract":"<div><div>Tick-borne encephalitis virus (TBEV) is a neurotropic orthoflavivirus responsible for severe infections of the central nervous system. Although neurons are predominantly targeted, specific involvement of microglia in pathogenesis of TBE is not yet fully understood. In this study, the susceptibility of human microglia to TBEV is investigated, focusing on productive infection and different immune responses of different viral strains. We investigated primary human microglia and two immortalized microglial cell lines exposed to three TBEV strains (Hypr, Neudörfl and 280), each differing in virulence. Our results show that all microglia cultures tested support long-term productive infections, regardless of the viral strain. In particular, immune response varied significantly with the viral strain, as shown by the differential secretion of cytokines and chemokines such as IP-10, MCP-1, IL-8 and IL-6, quantified using a Luminex 48-plex assay. The most virulent strain triggered the highest cytokine induction. Electron tomography revealed substantial ultrastructural changes in the infected microglia, despite the absence of cytopathic effects. These findings underscore the susceptibility of human microglia to TBEV and reveal strain-dependent variations in viral replication and immune responses, highlighting the complex role of microglia in TBEV-induced neuropathology and contribute to a deeper understanding of TBE pathogenesis and neuroinflammation.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105383"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trypanosoma cruzi Vps34 colocalizes with Beclin1 and plays a role in parasite invasion of the host cell by modulating the expression of a sub-group of trans-sialidases 克氏锥虫 Vps34 与 Beclin1 共定位，通过调节反式苷酸酶亚群的表达，在寄生虫入侵宿主细胞的过程中发挥作用。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105385

Carlos Alcides Nájera , Mercedes Soares-Silva , Fernando Y. Maeda , Wanderson Duarte DaRocha , Isabela Meneghelli , Ana Clara Mendes , Marina Ferreira Batista , Claudio Vieira Silva , José Franco da Silveira , Cristina M. Orikaza , Nobuko Yoshida , Viviane Grazielle Silva , Santuza Maria Ribeiro Teixeira , Daniella Castanheira Bartholomeu , Diana Bahia

{"title":"Trypanosoma cruzi Vps34 colocalizes with Beclin1 and plays a role in parasite invasion of the host cell by modulating the expression of a sub-group of trans-sialidases","authors":"Carlos Alcides Nájera , Mercedes Soares-Silva , Fernando Y. Maeda , Wanderson Duarte DaRocha , Isabela Meneghelli , Ana Clara Mendes , Marina Ferreira Batista , Claudio Vieira Silva , José Franco da Silveira , Cristina M. Orikaza , Nobuko Yoshida , Viviane Grazielle Silva , Santuza Maria Ribeiro Teixeira , Daniella Castanheira Bartholomeu , Diana Bahia","doi":"10.1016/j.micinf.2024.105385","DOIUrl":"10.1016/j.micinf.2024.105385","url":null,"abstract":"<div><div><em>Trypanosoma cruzi</em>, the etiological agent of Chagas' disease, can infect both phagocytic and non-phagocytic cells. <em>T. cruzi</em> gp82 and gp90 are cell surface proteins belonging to Group II <em>trans</em>-sialidases known to be involved in host cell binding and invasion. Phosphatidylinositol kinases (PIK) are lipid kinases that phosphorylate phospholipids in their substrates or in themselves, regulating important cellular functions such as metabolism, cell cycle and survival. Vps34, a class III PIK, regulates autophagy, trimeric G-protein signaling, and the mTOR (mammalian Target of Rapamycin) nutrient-sensing pathway. The mammalian autophagy gene Beclin1 interacts to Vps34 forming Beclin 1–Vps34 complexes involved in autophagy and protein sorting. In <em>T. cruzi</em> epimastigotes, (a <em>non</em>-infective replicative form), TcVps34 has been related to morphological and functional changes associated to vesicular trafficking, osmoregulation and receptor-mediated endocytosis. We aimed to characterize the role of TcVps34 during invasion of HeLa cells by metacyclic (MT) forms. MTs overexpressing TcVps34 showed lower invasion rates compared to controls, whilst exhibiting a significant decrease in gp82 expression in the parasite surface. In addition, we showed that <em>T. cruzi</em> Beclin (TcBeclin1) colocalizes with TcVps34 in epimastigotes, thus suggesting the formation of complexes that may play conserved cellular roles already described for other eukaryotes.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105385"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicles from primary human macrophages stimulated with VIP or PACAP mediate anti-SARS-CoV-2 activities in monocytes through NF-κB signaling pathway 受到 VIP 或 PACAP 刺激的原代人类巨噬细胞的细胞外囊泡通过 NF-κB 信号通路介导单核细胞的抗 SARS-CoV-2 活性。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105400

Luis A. Arteaga-Blanco , Jairo R. Temerozo , Lucas P.S. Tiné , Luíza Dantas-Pereira , Carolina Q. Sacramento , Natalia Fintelman-Rodrigues , Beatriz M. Toja , Suelen Silva Gomes Dias , Caroline S. de Freitas , Camila Couto Espírito-Santo , Ygor P. Silva , Rudimar L. Frozza , Patrícia T. Bozza , Rubem F.S. Menna-Barreto , Thiago Moreno L. Souza , Dumith Chequer Bou-Habib

{"title":"Extracellular vesicles from primary human macrophages stimulated with VIP or PACAP mediate anti-SARS-CoV-2 activities in monocytes through NF-κB signaling pathway","authors":"Luis A. Arteaga-Blanco , Jairo R. Temerozo , Lucas P.S. Tiné , Luíza Dantas-Pereira , Carolina Q. Sacramento , Natalia Fintelman-Rodrigues , Beatriz M. Toja , Suelen Silva Gomes Dias , Caroline S. de Freitas , Camila Couto Espírito-Santo , Ygor P. Silva , Rudimar L. Frozza , Patrícia T. Bozza , Rubem F.S. Menna-Barreto , Thiago Moreno L. Souza , Dumith Chequer Bou-Habib","doi":"10.1016/j.micinf.2024.105400","DOIUrl":"10.1016/j.micinf.2024.105400","url":null,"abstract":"<div><div>Infection by SARS-CoV-2 is associated with uncontrolled inflammatory response during COVID-19 severe disease, in which monocytes are one of the main sources of pro-inflammatory mediators leading to acute respiratory distress syndrome. Extracellular vesicles (EVs) from different cells play important roles during SARS-CoV-2 infection, but investigations describing the involvement of EVs from primary human monocyte-derived macrophages (MDM) on the regulation of this infection are not available. Here, we describe the effects of EVs released by MDM stimulated with the neuropeptides VIP and PACAP on SARS-CoV-2-infected monocytes. MDM-derived EVs were isolated by differential centrifugation of medium collected from cells cultured for 24 h in serum-reduced conditions. Based on morphological properties, we distinguished two subpopulations of MDM-EVs, namely large (LEV) and small EVs (SEV). We found that MDM-derived EVs stimulated with the neuropeptides inhibited SARS-CoV-2 RNA synthesis/replication in monocytes, protected these cells from virus-induced cytopathic effects and reduced the production of pro-inflammatory mediators. In addition, EVs derived from VIP- and PACAP-treated MDM prevented the SARS-CoV-2-induced NF-κB activation. Overall, our findings suggest that MDM-EVs are endowed with immunoregulatory properties that might contribute to the antiviral and anti-inflammatory responses in SARS-CoV-2-infected monocytes and expand our knowledge of EV effects during COVID-19 pathogenesis.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105400"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0