Microbes and Infection最新文献_第2页

Chlamydia pneumoniae relies on host glutathione for its growth and induces integrated stress response-mediated changes in macrophage glutathione metabolism 肺炎衣原体依赖宿主谷胱甘肽生长并诱导巨噬细胞谷胱甘肽代谢的综合应激反应介导的变化。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-05-01 DOI: 10.1016/j.micinf.2025.105501

Maarit Ylätalo, Eveliina Taavitsainen-Wahlroos, Inés Reigada, Leena Hanski

{"title":"Chlamydia pneumoniae relies on host glutathione for its growth and induces integrated stress response-mediated changes in macrophage glutathione metabolism","authors":"Maarit Ylätalo, Eveliina Taavitsainen-Wahlroos, Inés Reigada, Leena Hanski","doi":"10.1016/j.micinf.2025.105501","DOIUrl":"10.1016/j.micinf.2025.105501","url":null,"abstract":"<div><div>The obligate intracellular bacterium <em>Chlamydia pneumoniae</em> can enter into persistent phenotype, which is refractory to antibiotics and causes prolonged inflammatory state in the host. Molecular mechanisms enabling <em>C. pneumoniae</em> intracellular survival and governing the balance between persistent and productive infection phenotype remain poorly understood. In this study, the role of glutathione (GSH) metabolism in <em>C. pneumoniae</em> growth and progeny production was studied in THP-1 macrophages and A549 epithelial cells. Results indicate that depletion of cellular GSH pools decreased <em>C. pneumoniae</em> replication, but only if the constituent amino acids were also sequestered from the culture. <em>C. pneumoniae</em> infection increased the expression of GSH biosynthetic genes but also upregulated ChaC1, an intracellular enzyme involved in GSH breakage. <em>C. pneumoniae</em> infection was found to increase PERK phosphorylation in THP-1 macrophages and chemical inhibition of PERK prevented the infection-induced upregulation of GSH biosynthesis and GSH degradation genes and suppressed <em>C. pneumoniae</em> replication. <em>C. pneumoniae</em> -induced ChaC1 upregulation was also suppressed by protein kinase R inhibitor or treatment with ISRIB, indicating an involvement of redundant pathways of the host cell stress response. The data suggest that <em>C. pneumoniae</em> requires amino acids derived from the host cell GSH pools to enable active bacterial replication.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 4","pages":"Article 105501"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Host–parasite interactions after in vitro infection of human macrophages by Leishmania major: Dual analysis of microRNA and mRNA profiles reveals regulation of key processes through time kinetics 利什曼原虫体外感染人巨噬细胞后宿主与寄生虫的相互作用：microRNA和mRNA谱的双重分析揭示了通过时间动力学调节关键过程。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-05-01 DOI: 10.1016/j.micinf.2025.105502

Chiraz Atri , Ghada Mkannez , Hanène Attia , Rabiaa Manel Sghaier , Aymen Bali , Ali Ben-Cheikh , Imen Rabhi , Béatrice Regnault , David Piquemal , Kais Ghedira , Koussay Dellagi , Dhafer Laouini , Fatma Zahra Guerfali

{"title":"Host–parasite interactions after in vitro infection of human macrophages by Leishmania major: Dual analysis of microRNA and mRNA profiles reveals regulation of key processes through time kinetics","authors":"Chiraz Atri , Ghada Mkannez , Hanène Attia , Rabiaa Manel Sghaier , Aymen Bali , Ali Ben-Cheikh , Imen Rabhi , Béatrice Regnault , David Piquemal , Kais Ghedira , Koussay Dellagi , Dhafer Laouini , Fatma Zahra Guerfali","doi":"10.1016/j.micinf.2025.105502","DOIUrl":"10.1016/j.micinf.2025.105502","url":null,"abstract":"<div><div>Micro-RNAs are a class of small non-coding ribonucleic acids that concomitantly regulate the expression of tens to hundreds of genes. To reduce the host's defense, <em>Leishmania</em> parasites hijack the cellular functions of their macrophage's targets through gene expression regulation. Only few studies have attempted to correlate miRNAs and mRNAs expressions within the same samples in the context of cellular parasitism.</div><div>In this study, the profiling of human macrophages, <em>in vitro</em> infected by <em>L. major</em> parasites, was performed at both the mRNA transcriptomic level and the expression of a set of 365 miRNAs, and we correlated their expressions in search for a common molecular signature.</div><div>Both mRNA and miRNA profiles were monitored during the first 24 h post-infection to capture potential time-dependent fluctuations. We then cross-correlated the cellular biological processes and the pathways associated to the predicted targets of miRNAs and to the differentially expressed mRNAs at all time points of infection on the same samples.</div><div>Besides revealing the classical activation of immune signaling pathways, the mRNA-micro-RNAs correlation study highlighted other common regulatory inflammatory biological processes, allowing identification of rapidly modulated pathways, and bringing further evidence on the early molecular cross talk that take place between <em>Leishmania</em> and infected cells.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 4","pages":"Article 105502"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

No association between anti-cytomegalovirus seropositivity and arthritis: evidence from the cross-sectional epidemiology and genetic association analyses 抗巨细胞病毒血清阳性与关节炎之间无关联：来自横断面流行病学和遗传关联分析的证据。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-05-01 DOI: 10.1016/j.micinf.2025.105529

Changzhou Feng , Haining Li , Ying Zhou, Chu Zhang, Jin Yang, Haiqing Wang

{"title":"No association between anti-cytomegalovirus seropositivity and arthritis: evidence from the cross-sectional epidemiology and genetic association analyses","authors":"Changzhou Feng , Haining Li , Ying Zhou, Chu Zhang, Jin Yang, Haiqing Wang","doi":"10.1016/j.micinf.2025.105529","DOIUrl":"10.1016/j.micinf.2025.105529","url":null,"abstract":"<div><div>Human cytomegalovirus (CMV), a β-herpesvirus associated with chronic inflammation and lifelong latency, has been implicated in the pathogenesis of arthritis. However, the nature of this relationship remains controversial. In this study, we integrate cross-sectional epidemiology analyses, genetic correlation assessments, and Mendelian randomization (MR) approaches to elucidate the potential association between CMV infection and arthritis-related conditions. Observational analysis of 5133 participants from the NHANES database revealed a positive association between CMV IgG seropositivity and arthritis (OR: 1.24; 95 % CI: 1.03–1.48; <em>P</em> = 0.02), particularly with the rheumatoid arthritis (RA) subtype (OR: 1.94; 95 % CI: 1.21–3.12; <em>P</em> < 0.01). However, these associations lost statistical significance after adjustment for multiple covariates (all <em>P</em> > 0.05). Subgroup and interaction analyses across different demographic and clinical subpopulations further confirmed the absence of these associations. Similarly, subtype analyses indicated no significant association between CMV IgG seropositivity and osteoarthritis (OA), other-arthritis, or unknown-arthritis, even before covariate adjustment. Linkage disequilibrium score regression (LDSC) analysis did not reveal a significant genetic correlation between anti-CMV IgG levels and arthritis, including RA and OA (all <em>P</em> > 0.05), suggesting no shared genetic basis. Furthermore, bidirectional MR analyses found no evidence of a causal relationship between CMV antibody responses—including IgG and three CMV-related peptide antigens (pp28, pp52, and pp150)—and arthritis, RA, or OA (all <em>P</em> > 0.05). Collectively, these findings suggest that previously reported positive associations between CMV seropositivity and arthritis may have been confounded by other covariates rather than reflecting a true causal relationship.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 4","pages":"Article 105529"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chlamydia trachomatis regulates ferroptosis through the p53/SLC7A11 pathway to promote reproduction 沙眼衣原体通过p53/SLC7A11通路调控铁下垂，促进生殖。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-05-01 DOI: 10.1016/j.micinf.2025.105505

Xinglv Wang , Chengyu Tang , Hongrong Wu , Jingrong Zhang , Lili Chen , Zhongyu Li

{"title":"Chlamydia trachomatis regulates ferroptosis through the p53/SLC7A11 pathway to promote reproduction","authors":"Xinglv Wang , Chengyu Tang , Hongrong Wu , Jingrong Zhang , Lili Chen , Zhongyu Li","doi":"10.1016/j.micinf.2025.105505","DOIUrl":"10.1016/j.micinf.2025.105505","url":null,"abstract":"<div><div>Genital tract <em>Chlamydia trachomatis (Ct)</em> infection is one of the most prevalent sexually transmitted infections (STIs) worldwide. However, its clinical progression is often insidious and prolonged. Understanding the mechanisms by which <em>Ct</em> influences cell death pathways is crucial for elucidating the pathogenic processes of this intracellular bacterium. Ferroptosis, a newly identified form of programmed cell death, is characterized by the iron-dependent accumulation of lipid peroxides. Despite its relevance, the interaction between <em>Ct</em> and ferroptosis remains poorly studied. In the present study, we first performed bioinformatics analysis based on RNA sequencing data under an in vitro model of <em>Ct</em> acute infection. Bioinformatics analysis revealed significant enrichment of differentially expressed genes in ferroptosis and p53 signaling pathways. Subsequently, we validated the hypothesis that <em>Ct</em> inhibits host ferroptosis by expression assays of ferroptosis-related proteins. Further cell proliferation, intracellular ferrous iron fluorescence, and lipid peroxidation assays multifaceted observations of the phenotype. Mechanistically, we found that <em>Ct</em> inhibition of ferroptosis acts by regulating the host p53/SLC7A11 pathway. Finally, indirect immunofluorescence assays demonstrated that ferroptosis decreases inclusion forming units (IFUs) of <em>Ct</em> progeny and thus affects its reproduction, which partly explains <em>Ct</em>'s survival strategy of resisting host ferroptosis.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 4","pages":"Article 105505"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative pathoadaptation of Mycobacterium canettii and Mycobacterium tuberculosis: Insights from assays on phagosome acidification, cytosolic access, and transcriptomics canettii分枝杆菌和结核分枝杆菌的比较病理适应：从吞噬体酸化、细胞质通路和转录组学分析的见解。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-05-01 DOI: 10.1016/j.micinf.2025.105503

Camila do Nascimento Araujo, Felipe Silva, Cristina Kraemer Zimpel , Taiana Tainá Silva-Pereira, Naila Cristina Soler-Camargo, Filipe Menegatti de Melo , Marcelo Valdemir de Araújo , Ana Marcia de Sá Guimarães

{"title":"Comparative pathoadaptation of Mycobacterium canettii and Mycobacterium tuberculosis: Insights from assays on phagosome acidification, cytosolic access, and transcriptomics","authors":"Camila do Nascimento Araujo, Felipe Silva, Cristina Kraemer Zimpel , Taiana Tainá Silva-Pereira, Naila Cristina Soler-Camargo, Filipe Menegatti de Melo , Marcelo Valdemir de Araújo , Ana Marcia de Sá Guimarães","doi":"10.1016/j.micinf.2025.105503","DOIUrl":"10.1016/j.micinf.2025.105503","url":null,"abstract":"<div><div>Genetic and molecular differences between <em>Mycobacterium tuberculosis</em> (Mtb) and its ancestral counterpart, <em>Mycobacterium canettii</em> (Mcan), remain poorly known. Our study aimed to compare their modulation of phagosome acidification and cytosolic access in macrophages, and their <em>in vitro</em> transcriptomes. Using spectrofluorometry, we tracked pH changes in mycobacteria-containing vacuoles in THP-1 macrophages. A single-cell FRET protocol evaluated cytosolic access of mycobacteria in these cells. Similar to Mtb, Mcan inhibits phagosome acidification and accesses the cytosol. Transcriptomic and genetic analyses reveal mutations in two-component systems (PhoPR, SenX3-RegX3, and DevRS/DosRS) and in specific genes (e.g., lactate dehydrogenase and <em>espACD</em>) driving variations in gene expression between pathogens. Moreover, Mcan upregulates genes of iron and molybdopterin metabolism compared to Mtb, suggesting a role for metals in the evolution of tuberculous mycobacteria. The upregulation of the termination factor Rho in Mtb also suggests differences in antisense transcription and/or gene expression regulation. In conclusion, phagosome modulation and cytosolic access in macrophages are ancestral traits predating the emergence of the MTBC and not exclusive to Mtb's strict pathogenic lifestyle. Additionally, gene expression regulation likely shaped the phenotypic differences between Mcan and Mtb, contributing to the evolutionary transition from an environmental Mcan-like ancestor to the MTBC's host-adapted lifestyle.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 4","pages":"Article 105503"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas aeruginosa-derived DnaJ induces TLR2 expression through TLR10-mediated activation of the PI3K-SGK1 pathway in macrophages 铜绿假单胞菌衍生的DnaJ通过tlr10介导的巨噬细胞PI3K-SGK1通路激活诱导TLR2表达。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-05-01 DOI: 10.1016/j.micinf.2025.105481

Jaehoo Lee , Yongxin Jin , Weihui Wu , Yeji Lee , Un-Hwan Ha

{"title":"Pseudomonas aeruginosa-derived DnaJ induces TLR2 expression through TLR10-mediated activation of the PI3K-SGK1 pathway in macrophages","authors":"Jaehoo Lee , Yongxin Jin , Weihui Wu , Yeji Lee , Un-Hwan Ha","doi":"10.1016/j.micinf.2025.105481","DOIUrl":"10.1016/j.micinf.2025.105481","url":null,"abstract":"<div><div>TLR2 is a key component of the innate immune system, responsible for recognizing Gram-positive bacterial components and initiating inflammatory signaling cascades that activate defense responses. However, little is known about the regulatory effects of <em>Pseudomonas aeruginosa</em> (<em>P. aeruginosa</em>) on TLR2 expression. In this study, we investigated the potential link between <em>P. aeruginosa</em>-derived DnaJ and TLR2 expression in macrophages, as well as the activation of downstream signaling pathways. Our findings revealed that DnaJ significantly induced TLR2 expression in a dose- and time-dependent manner, predominantly affecting TLR2 with minimal impact on other TLRs, such as TLR4 and TLR5, which detect bacterial PAMPs. The DnaJ-mediated TLR2 induction was driven by activation of the PI3K-SGK1 signaling pathway, with TLR10 playing a crucial role in facilitating these effects. This increase in TLR2 expression led to enhanced production of inflammatory cytokines in response to secondary <em>Staphylococcus aureus</em> infections, indicating a role in boosting host defense mechanisms. In conclusion, these findings suggest that <em>P. aeruginosa</em>-derived DnaJ promotes TLR2 expression via TLR10-mediated activation of the PI3K-SGK1 pathway, thereby enhancing host immune responses against Gram-positive bacterial infections.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 4","pages":"Article 105481"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nano-enhanced benzylpenicillin: Bridging antibacterial action with anti-inflammatory potential against antibiotic-resistant bacteria 纳米增强型苄青霉素：抗生素耐药细菌的抗菌作用与消炎潜力的桥梁

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105436

Natália Cristina Gomes-da-Silva , Álefe Roger Silva França , Clenilton Costa dos Santos , Luciana Magalhães Rebelo Alencar , Elaine Cruz Rosas , Luana Barbosa Corrêa , Carolline M.A. Lorentino , André L.S. Santos , Eduardo Ricci-Junior , Ralph Santos-Oliveira

{"title":"Nano-enhanced benzylpenicillin: Bridging antibacterial action with anti-inflammatory potential against antibiotic-resistant bacteria","authors":"Natália Cristina Gomes-da-Silva , Álefe Roger Silva França , Clenilton Costa dos Santos , Luciana Magalhães Rebelo Alencar , Elaine Cruz Rosas , Luana Barbosa Corrêa , Carolline M.A. Lorentino , André L.S. Santos , Eduardo Ricci-Junior , Ralph Santos-Oliveira","doi":"10.1016/j.micinf.2024.105436","DOIUrl":"10.1016/j.micinf.2024.105436","url":null,"abstract":"<div><div>This study investigates the enhancement of benzylpenicillin's antibacterial properties using nanomedicine, specifically by developing benzylpenicillin nanoemulsions. To address the escalating issue of bacterial resistance, we employed the advanced techniques Raman spectroscopy and atomic force microscopy to analyze the nanoemulsions' molecular structure and characteristics. We then evaluated the impact of these nanoemulsions on nitric oxide production by macrophages to deternine their potential to modulate inflammatory responses. We further assessed the antibacterial effectiveness of the nanoparticles against the pathogens <em>Streptococcus pyogenes</em> (Group A <em>Streptococcus</em>) and <em>Streptococcus agalactiae</em> (Group B <em>Streptococcus</em>). The results of antibiograms showed significant efficacy against Gram-positive bacteria, with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values, confirming their bactericidal potential. The investigation into the mechanism of action suggested substantial disruption to bacterial membrane integrity, underscoring a possible mode of antibacterial activity. Overall, the study provides valuable insights into the synergistic relationship between antibiotics and nanoparticles. In particular, it demonstrates the potential of benzylpenicillin nanoparticles to enhance the antimicrobial efficacy and influence inflammatory responses obtained by evaluating nitrite, IL-6 and TNF-α, offering promising avenues for future clinical applications and strategies to combat bacterial resistance.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105436"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photodynamic therapy reduces viability, enhances itraconazole activity, and impairs mitochondrial physiology of Sporothrix brasiliensis 光动力疗法降低了巴西孢子虫的活力，增强了伊曲康唑的活性，并损害了线粒体的生理机能。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105440

Mariana Lucy Mesquita Ramos , Azuil Barrinha , Glauber Ribeiro de Sousa Araújo , Vinicius Alves , Iara Bastos de Andrade , Dario Corrêa-Junior , Maria Cristina Machado Motta , Rodrigo Almeida-Paes , Susana Frases

{"title":"Photodynamic therapy reduces viability, enhances itraconazole activity, and impairs mitochondrial physiology of Sporothrix brasiliensis","authors":"Mariana Lucy Mesquita Ramos , Azuil Barrinha , Glauber Ribeiro de Sousa Araújo , Vinicius Alves , Iara Bastos de Andrade , Dario Corrêa-Junior , Maria Cristina Machado Motta , Rodrigo Almeida-Paes , Susana Frases","doi":"10.1016/j.micinf.2024.105440","DOIUrl":"10.1016/j.micinf.2024.105440","url":null,"abstract":"<div><div><em>Sporothrix brasiliensis</em> is the main agent of sporotrichosis in Brazil, with few therapeutic options. This study aimed to investigate the <em>in vitro</em> efficacy of photodynamic therapy using a diode laser (InGaAIP) in combination with the photosensitizer methylene blue against <em>S. brasiliensis</em> yeasts. Additionally, we evaluated the underexplored mitochondrial activity of <em>S. brasiliensis</em> and the impact of laser treatment on the fungal mitochondrial aspects post-treatment. Three strains of <em>S. brasiliensis</em> were used, including a non-wild-type strain to itraconazole. Yeast viability was determined by counting colony-forming units. For a comprehensive analysis of irradiated versus non-irradiated cells, we assessed combined therapy with itraconazole, scanning electron microscopy of cells, and mitochondrial activity. The latter included high-resolution respirometry, membrane potential analysis, and reactive oxygen species production. Methylene blue combined with photodynamic therapy inhibited the growth of the isolates, including the non-wild-type strain to itraconazole. Photodynamic therapy induced the production of reactive oxygen species, which negatively affected mitochondrial function, resulting in decreased membrane potential and cell death. Photodynamic therapy altered the ultrastructure and mitochondrial physiology of <em>S. brasiliensis</em>, suggesting a new therapeutic approach for sporotrichosis caused by this species.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105440"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142668089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of a monoclonal antibody targeting the SARS-CoV-2 Omicron spike protein and analysis of SARS-CoV-2 Omicron mutations related to monoclonal antibody resistance 生产针对 SARS-CoV-2 Omicron 穗蛋白的单克隆抗体，分析与单克隆抗体抗性有关的 SARS-CoV-2 Omicron 突变。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105461

Jinsoo Kim , Suyeon Kim , Sangkyu Park , Dongbum Kim , Minyoung Kim , Kyeongbin Baek , Bo Min Kang , Ha-Eun Shin , Myeong-Heon Lee , Younghee Lee , Hyung-Joo Kwon

{"title":"Production of a monoclonal antibody targeting the SARS-CoV-2 Omicron spike protein and analysis of SARS-CoV-2 Omicron mutations related to monoclonal antibody resistance","authors":"Jinsoo Kim , Suyeon Kim , Sangkyu Park , Dongbum Kim , Minyoung Kim , Kyeongbin Baek , Bo Min Kang , Ha-Eun Shin , Myeong-Heon Lee , Younghee Lee , Hyung-Joo Kwon","doi":"10.1016/j.micinf.2024.105461","DOIUrl":"10.1016/j.micinf.2024.105461","url":null,"abstract":"<div><div>SARS-CoV-2 mutations have resulted in the emergence of multiple concerning variants, with Omicron being the dominant strain presently. Therefore, we developed a monoclonal antibody (mAb) against the spike (S) protein of SARS-CoV-2 Omicron for therapeutic applications. We established the 1E3H12 mAb, recognizing the receptor binding domain (RBD) of the Omicron S protein, and found that the 1E3H12 mAb can efficiently recognize the Omicron S protein with weak affinity to the Alpha, Beta, and Mu variants, but not to the parental strain and Delta variant. Based on <em>in vitro</em> assays, the mAb demonstrated neutralizing activity against Omicron BA.1, BA.4/5, BQ.1.1, and XBB. A humanized antibody was further produced and proved to have neutralizing activity. To verify the potential limitations of the 1E3H12 mAb due to viral escape of SARS-CoV-2 Omicron variants, we analyzed the emergence of variants by whole genome deep sequencing after serial passage in cell culture. The results showed a few unique S protein mutations in the genome associated with resistance to the mAb. These findings suggest that this antibody not only contributes to the therapeutic arsenal against COVID-19 but also addresses the ongoing challenge of antibody resistance among the evolving subvariants of SARS-CoV-2 Omicron.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105461"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copyright page Elsevier 版权页面Elsevier

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/S1286-4579(25)00057-7

引用次数: 0