Microbes and Infection最新文献_第2页

Cross-species transmission and animal infection model of hepatitis E virus 戊型肝炎病毒的跨物种传播和动物感染模型。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2024.105338

Ling-Dong Xu , Fei Zhang , Pinglong Xu , Yao-Wei Huang

{"title":"Cross-species transmission and animal infection model of hepatitis E virus","authors":"Ling-Dong Xu , Fei Zhang , Pinglong Xu , Yao-Wei Huang","doi":"10.1016/j.micinf.2024.105338","DOIUrl":"10.1016/j.micinf.2024.105338","url":null,"abstract":"<div><div>Zoonotic hepatitis E virus (HEV) infection is an emerging global public health concern, and understanding the dynamics of HEV transmission between animals and humans is crucial for public health. Animal models are critical to advancing the understanding of HEV pathogenesis, drug screening, vaccine development, and other related areas. Here, we provide an overview of recent studies investigating the cross-species transmission of HEV, and also delve into the current research and application of animal HEV infection models including non-human primates, rodents, pigs, and chickens, offering a comprehensive assessment of the advantages and disadvantages of each model. This review highlights the findings related to viral replication, shedding patterns, and immune response in these animal models, and discusses the implications for our understanding of HEV transmission to humans. These advancements in the field enhance our understanding of the biological traits and pathogenic mechanisms of HEV, offering robust support for the development of highly effective and targeted prevention and treatment strategies.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105338"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140781412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dual M protein systems have diverse biological characteristics, but both contribute to M18-type Group A Streptococcus pathogenicity 双M蛋白系统具有不同的生物学特性，但两者都有助于M18型A组链球菌的致病性。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2023.105209

Xiaorui Zhou , Haoshuai Song , Fei Pan , Chen Yuan , Lu Jia , Bing Wu , Hongjie Fan , Zhe Ma

{"title":"The dual M protein systems have diverse biological characteristics, but both contribute to M18-type Group A Streptococcus pathogenicity","authors":"Xiaorui Zhou , Haoshuai Song , Fei Pan , Chen Yuan , Lu Jia , Bing Wu , Hongjie Fan , Zhe Ma","doi":"10.1016/j.micinf.2023.105209","DOIUrl":"10.1016/j.micinf.2023.105209","url":null,"abstract":"<div><div>M protein is a key surface virulence factor in Group A Streptococcus (GAS), Group C Streptococcus (GCS), and other streptococcal species. GAS encodes M protein using the <em>emm</em> gene, while GCS employs the <em>szm</em> (or <em>sem</em>) gene. In M18-type GAS, dual M protein systems exist, comprising both GAS and GCS M proteins (encoded separately by <em>emm</em>18 and <em>spa</em>18). The <em>spa</em>18 gene in M18-type GAS shares a conserved region highly similar to GCS's <em>szm</em> gene. Our study reveals that <em>spa</em>18 exhibits higher transcription levels than <em>emm</em>18 in M18-type GAS strains. The dual M protein systems defective mutant (Δemm18Δspa18) displays a smooth surface, whereas wild-type and single M protein gene mutants remain rough. M18 and SPA18 proteins possess distinct characteristics, showing varied binding properties and cytotoxicity effects on macrophages (THP-1) and keratinocytes (HaCaT). Both <em>emm</em>18 and <em>spa</em>18 genes contribute to the skin pathogenicity of M18-type GAS. Transcriptome analysis suggests the potential involvement of the <em>mga</em> gene in <em>spa</em>18 transcription regulation, while <em>SpyM</em>18_2047 appears to be specific to <em>spa</em>18 regulation. In summary, this research offers a crucial understanding of the biological characteristics of dual M protein systems in M18-type GAS, highlighting their contributions to virulence and transcriptional regulation.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105209"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10030163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rab27a via its effector JFC1 localizes to Anaplasma inclusions and promotes Anaplasma proliferation in leukocytes Rab27a 通过其效应物 JFC1 定位于阿那普拉斯原虫包涵体并促进阿那普拉斯原虫在白细胞中的增殖

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2023.105278

Weiyan Huang, Mingqun Lin, Yasuko Rikihisa

{"title":"Rab27a via its effector JFC1 localizes to Anaplasma inclusions and promotes Anaplasma proliferation in leukocytes","authors":"Weiyan Huang, Mingqun Lin, Yasuko Rikihisa","doi":"10.1016/j.micinf.2023.105278","DOIUrl":"10.1016/j.micinf.2023.105278","url":null,"abstract":"<div><div><em>Anaplasma phagocytophilum</em> is an obligatory intracellular bacterium that causes tick-borne zoonosis called human granulocytic anaplasmosis. Mechanisms by which <em>Anaplasma</em> replicates inside of the membrane-bound compartment called “inclusion” in neutrophils are incompletely understood. A small GTPase Rab27a is found in the secretory granules and multivesicular endosomes. In this study we found Rab27a-containing granules were localized to <em>Anaplasma</em> inclusions in guanine nucleotide-dependent manner, and constitutively active Rab27a enhanced <em>Anaplasma</em> infection and dominant-negative Rab27a inhibited <em>Anaplasma</em> infection. Rab27a effector, JFC1 is known to mediate docking/fusion of Rab27a-bearing granules for exocytosis in leukocytes. shRNA stable knockdown of Rab27a or JFC1 inhibited <em>Anaplasma</em> infection in HL-60 cells. Similar to Rab27a, both endogenous and transfected JFC1 were localized to <em>Anaplasma</em> inclusions by immunostaining or live cell imaging. The JFC1 C2A domain that binds 3′-phosphoinositides, was sufficient and required for JFC1 and Rab27a localization to <em>Anaplasma</em> inclusions which were enriched with phosphatidylinositol 3-phosphate. Nexinhib20, the small molecule inhibitor specific to Rab27a and JFC1 binding, inhibited <em>Anaplasma</em> infection. Taken together, these results imply elevated phosphatidylinositol 3-phosphate in the inclusion membrane recruits JFC1 to mediate Rab27a-bearing granules/vesicles to dock/fuse with <em>Anaplasma</em> inclusions, the lumen of which is topologically equivalent to the exterior of the cell to benefit <em>Anaplasma</em> proliferation.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105278"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138682491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The functions of EF-hand proteins from host and zoonotic pathogens 宿主和人畜共患病原体的 EF-手蛋白的功能

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2023.105276

Wei Zuo , Mingxing Tian , Jingjing Qi , Guangdong Zhang , Jiangang Hu , Shaohui Wang , Yanqing Bao

引用次数: 0

Lineage classification and selective site identification of Orthoebolavirus zairense Orthoebolavirus zairense 的品系分类和选择性位点鉴定。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2024.105304

Jie Fang , Zhi-jian Zhou , Shuofeng Yuan , Ye Qiu , Xing-Yi Ge

{"title":"Lineage classification and selective site identification of Orthoebolavirus zairense","authors":"Jie Fang , Zhi-jian Zhou , Shuofeng Yuan , Ye Qiu , Xing-Yi Ge","doi":"10.1016/j.micinf.2024.105304","DOIUrl":"10.1016/j.micinf.2024.105304","url":null,"abstract":"<div><div>As the high pathogenic species of <em>Filoviridae</em> virus family, <em>Orthoebolavirus zairense</em> (EBOV) shows frequent outbreaks in human in recently years since its first emerging in 1976 in Democratic Republic of the Congo (COD), bringing ongoing risks and burden on public health safety. Here, the phylogenetic relationship among major outbreaks was analyzed. The results showed that EBOV isolates could be divided into four lineages according to spatial and temporal epidemics. Then, the positive selection sites (PSSs) were detected on all proteins of the EBOV, exhibiting lineage characteristic. Particularly, sites in GP and VP24 were identified to be significantly under positive selection, and partial of which were maintained in the latest isolates in 2021. GP and L were found to have high variability between lineages. Substitutions including F443L and F443S in GP, as well as F1610L and I1951V in L could be characteristic of the two large outbreaks in COD (2018) and West Africa (2014), respectively. Further, substitutions of significant PSSs in VP24 and L proteins were visualized for analysis of structural changes, which may affect EBOV pathogenesis. In summary, our results gains insights in genetic characteristic and adaptive evolution of EBOV, which could facilitate gene functional research against EBOV.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105304"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139567237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycerol metabolic repressor GlpR contributes to Streptococcus suis oxidative stress resistance and virulence 甘油代谢抑制因子 GlpR 对猪链球菌抗氧化压力和病毒性有贡献

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2024.105307

Zijing Liang , Jiaxuan Lu , Yinli Bao , Xiang Chen , Huochun Yao , Zongfu Wu

{"title":"Glycerol metabolic repressor GlpR contributes to Streptococcus suis oxidative stress resistance and virulence","authors":"Zijing Liang , Jiaxuan Lu , Yinli Bao , Xiang Chen , Huochun Yao , Zongfu Wu","doi":"10.1016/j.micinf.2024.105307","DOIUrl":"10.1016/j.micinf.2024.105307","url":null,"abstract":"<div><div>Bacterial DeoR family transcription regulators regulate multiple physiological processes. Little is known about the function of DeoR family regulators in streptococci. Here, we identified a novel DeoR family regulator, GlpR, from <em>Streptococcus suis</em>, a pathogen causing severe diseases in pigs and humans. GlpR was involved in glycerol utilization and exhibited specific signature residues at positions 30–31 (KV) which are crucial for DNA binding. Deletion of <em>glpR</em> (Δ<em>glpR</em>) showed a significant increase in relative growth rate in glycerol medium compared to the wild-type (WT) and complementary strains (CΔ<em>glpR</em>). Employing RNA-seq analysis, β-galactosidase activity analysis, and electrophoretic mobility shift assay, we discovered that GlpR directly represses the expression of glycerol metabolism-related genes <em>pflB2</em>, <em>pflA1</em>, and <em>fsaA</em>, encoding pyruvate formate-lyase and its activating enzyme, and fructose-6-phosphate aldolase, respectively. Compared to WT and CΔ<em>glpR</em>, Δ<em>glpR</em> showed a reduced survival rate under oxidative stress and in murine macrophages and attenuated virulence in mice. GlpR probably enhances oxidative stress resistance and virulence in <em>S. suis</em> by functioning as a glycerol metabolic repressor decreasing energy consumption. These findings contribute to a better understanding of <em>S. suis</em> pathogenesis and enrich our knowledge of the biological functions of DeoR family regulators in streptococci.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105307"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139662958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of two-component system ArcAB and the universal stress protein E in Pasteurella multocida and their effects on bacterial fitness and pathogenesis 多杀性巴氏杆菌双组分系统ArcAB和通用应激蛋白E的鉴定及其对细菌适应度和发病机制的影响。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2023.105235

Qingjie Lv , Yuyao Shang , Haixin Bi , Jie Yang , Lin Lin , Congcong Shi , Mixue Wang , Rui Xie , Zhanwei Zhu , Fei Wang , Lin Hua , Huanchun Chen , Bin Wu , Zhong Peng

{"title":"Identification of two-component system ArcAB and the universal stress protein E in Pasteurella multocida and their effects on bacterial fitness and pathogenesis","authors":"Qingjie Lv , Yuyao Shang , Haixin Bi , Jie Yang , Lin Lin , Congcong Shi , Mixue Wang , Rui Xie , Zhanwei Zhu , Fei Wang , Lin Hua , Huanchun Chen , Bin Wu , Zhong Peng","doi":"10.1016/j.micinf.2023.105235","DOIUrl":"10.1016/j.micinf.2023.105235","url":null,"abstract":"<div><div>Two-component regulatory system (TCS) is a widespread bacterial signal transduction mechanism and plays a critical role in bacterial adaptation to environments as well as regulating bacterial virulence. However, few studies have reported the actions of TCS in <em>Pasteurella multocida</em>, a zoonotic bacterial pathogen<em>.</em> In this study, genes encoding proteins homologous to the ArcAB TCS were identified in genome sequences of <em>P. multocida</em> belonging to different serogroups, and the transcription of both <em>arcA</em> and <em>arcB</em> was up-regulated in anaerobic and superoxygen environment. Compared to wild type strains, <em>P. multocida arcA</em>-deletion mutants (ΔarcA) displayed a decrease in growing under anaerobic conditions, biofilm formation, as well as the capacities of anti-serum bactericidal effect, cell adherence and invasion, anti-phagocytosis, and virulence in different <em>in vivo</em> models (<em>Galleria mellonella</em> and mice). RNA-Seq identified 70 significantly downregulated genes in ΔarcA compared to the wild type strain, and several of them are associated with <em>P. multocida</em> virulence. Among them, a universal stress protein E encoding gene <em>uspE</em> was characterized in <em>P. multocida</em> for the first time. Electrophoretic mobility shift assay (EMSA) demonstrated that the ArcAB TCS could regulate <em>uspE</em> directly. Deletion of <em>uspE</em> also led to a decrease of <em>P. multocida</em> in growing under anaerobic conditions, biofilm formation, anti-serum bactericidal effect, cell adherence and invasion, anti-phagocytosis, and virulence in mice. The data provided from this study will help further understanding the fitness and pathogenesis of <em>P. multocida</em>.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105235"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41132917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gingivitis- and periodontitis-associated microbiota in bovine deciduous incisor teeth - A preliminary study. 牛乳牙门牙牙龈炎和牙周炎相关微生物群的初步研究。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-12-30 DOI: 10.1016/j.micinf.2024.105468

Juliana Vaccari, Ana C Borsanelli, Flávia R F Athayde, Júlia R Saraiva, Thamiris N M Ramos, Iveraldo S Dutra

{"title":"Gingivitis- and periodontitis-associated microbiota in bovine deciduous incisor teeth - A preliminary study.","authors":"Juliana Vaccari, Ana C Borsanelli, Flávia R F Athayde, Júlia R Saraiva, Thamiris N M Ramos, Iveraldo S Dutra","doi":"10.1016/j.micinf.2024.105468","DOIUrl":"10.1016/j.micinf.2024.105468","url":null,"abstract":"<p><p>As ruminants are frequently affected by periodontal diseases, understanding their microbial communities is crucial. In this pilot study, we analyzed subgingival biofilm samples of young cattle across different states: clinically healthy (n = 5), gingivitis (n = 5), and periodontitis (n = 5) using 16S rRNA gene sequencing and co-occurrence network analysis. The findings revealed that Proteobacteria was the predominant phylum across all conditions, with Fusobacteriota constituting 27.6 % of the microbiota in periodontitis-affected sites. In healthy sites, Moraxella (21.11 %), Neisseria (13.16 %), and Lautropia (7.69 %) were the predominant genera; in gingivitis-affected sites, the leading genera were Neisseria (23.65 %), Moraxella (18.95 %), and Conchiformibius (10.79 %); and in periodontitis sites, Caviibacter (19.78 %), Moraxella (16.13 %), and Fusobacterium (7.56 %) were most prevalent. Richness and dissimilarity analyses did not show significant differences across the clinical states, but differences were found between gingivitis and periodontitis sites (p = 0.01) in diversity. The co-occurrence networks highlighted significant variances in the central phyla across the phenotypes, with a higher number of positive interactions observed in periodontitis-affected sites. Consequently, this study demonstrated that the microbiota associated with periodontitis in young cattle exhibits greater diversity compared to gingivitis. Notably, in the deciduous dentition of cattle, the genera Caviibacter and Moraxella are pivotal in the context of periodontitis and periodontal health, respectively.</p>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105468"},"PeriodicalIF":2.6,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting the concept of giant viruses. 重新审视巨型病毒的概念。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-12-24 DOI: 10.1016/j.micinf.2024.105467

Jônatas Santos Abrahão

引用次数: 0

HERV reactivation by adenovirus infection is associated with viral immune regulation. "腺病毒感染导致的 HERV 再激活与病毒免疫调节有关"。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-12-21 DOI: 10.1016/j.micinf.2024.105466

Wen Liang, Miona Stubbe, Lisa Pleninger, Anna Hofferek, Hans Stubbe, Julia Mai, Salih Özer, Dmitrij Frishman, Sabrina Schreiner, Michelle Vincendeau

引用次数: 0