Microbes and Infection最新文献

{"title":"Production of a monoclonal antibody targeting the SARS-CoV-2 Omicron spike protein and analysis of SARS-CoV-2 Omicron mutations related to monoclonal antibody resistance","authors":"Jinsoo Kim ,&nbsp;Suyeon Kim ,&nbsp;Sangkyu Park ,&nbsp;Dongbum Kim ,&nbsp;Minyoung Kim ,&nbsp;Kyeongbin Baek ,&nbsp;Bo Min Kang ,&nbsp;Ha-Eun Shin ,&nbsp;Myeong-Heon Lee ,&nbsp;Younghee Lee ,&nbsp;Hyung-Joo Kwon","doi":"10.1016/j.micinf.2024.105461","DOIUrl":"10.1016/j.micinf.2024.105461","url":null,"abstract":"<div><div>SARS-CoV-2 mutations have resulted in the emergence of multiple concerning variants, with Omicron being the dominant strain presently. Therefore, we developed a monoclonal antibody (mAb) against the spike (S) protein of SARS-CoV-2 Omicron for therapeutic applications. We established the 1E3H12 mAb, recognizing the receptor binding domain (RBD) of the Omicron S protein, and found that the 1E3H12 mAb can efficiently recognize the Omicron S protein with weak affinity to the Alpha, Beta, and Mu variants, but not to the parental strain and Delta variant. Based on <em>in vitro</em> assays, the mAb demonstrated neutralizing activity against Omicron BA.1, BA.4/5, BQ.1.1, and XBB. A humanized antibody was further produced and proved to have neutralizing activity. To verify the potential limitations of the 1E3H12 mAb due to viral escape of SARS-CoV-2 Omicron variants, we analyzed the emergence of variants by whole genome deep sequencing after serial passage in cell culture. The results showed a few unique S protein mutations in the genome associated with resistance to the mAb. These findings suggest that this antibody not only contributes to the therapeutic arsenal against COVID-19 but also addresses the ongoing challenge of antibody resistance among the evolving subvariants of SARS-CoV-2 Omicron.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105461"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142695497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copyright page Elsevier","authors":"","doi":"10.1016/S1286-4579(25)00057-7","DOIUrl":"10.1016/S1286-4579(25)00057-7","url":null,"abstract":"","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105525"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intranasal immunization with poly I:C and CpG ODN adjuvants enhances the protective efficacy against Helicobacter pylori infection in mice","authors":"Min Sun,&nbsp;Yu Liu,&nbsp;Xiumei Ni,&nbsp;Runqing Tan,&nbsp;Yi Wang,&nbsp;Yajun Jiang,&nbsp;Dingxin Ke,&nbsp;Han Du,&nbsp;Gang Guo,&nbsp;Kaiyun Liu","doi":"10.1016/j.micinf.2024.105433","DOIUrl":"10.1016/j.micinf.2024.105433","url":null,"abstract":"<div><div><em>Helicobacter pylori</em> (<em>H. pylori</em>) infection is a serious public health issue, and development of vaccines is a desirable preventive strategy for <em>H. pylori</em>. Toll-like receptor (TLR) ligands have shown potential as vaccine adjuvants that induce immune responses, but polyinosinic-polycytidylic acid (poly I:C), a nucleic acid-based TLR9 ligand, is less well studied in <em>H. pylori</em> vaccine research. Here, we evaluated the effects of poly I:C and CpG oligodeoxynucleotide (CpG ODN), a nucleic acid TLR3 ligand, as adjuvants in combination with the <em>H. pylori</em> recombinant proteins LpoB and UreA to protect against <em>H. pylori</em> infection. For analysis of specific immune responses, the levels of specific antibodies and splenic cytokines were measured in the immunized mice. Compared with CpG ODN, poly I:C could induce mucosal sIgA antibody responses and reduce <em>H. pylori</em> colonization. Additionally, the combination of poly I:C and CpG ODN caused greater immunoprotection and significantly reduced gastritis, exerting synergistic effects. Analysis of splenic cytokines revealed that poly I:C mainly triggered a mixed Th1/Th2/Th17 immune response, whereas the combination of CpG ODN and poly I:C induced a Th1/Th17 immune response. Our findings indicated that increased levels of mucosal sIgA antibodies and a robust splenic Th1/Th17 immune response were associated with reduced <em>H. pylori</em> colonization in vaccinated mice. This study identified a potential TLR ligand adjuvant for developing more effective <em>H. pylori</em> vaccines.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105433"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miRNAs regulate the metabolic adaptation of Paracoccidioides brasiliensis during copper deprivation","authors":"Dener Lucas Araújo dos Santos ,&nbsp;Juliana Santana de Curcio ,&nbsp;Evandro Novaes ,&nbsp;Célia Maria de Almeida Soares","doi":"10.1016/j.micinf.2024.105435","DOIUrl":"10.1016/j.micinf.2024.105435","url":null,"abstract":"<div><div>Copper is an essential metal for cellular processes such as detoxification of reactive oxygen species, oxidative phosphorylation, and iron uptake. However, during infection, the host restricts the bioavailability of this micronutrient to the pathogen as a strategy to combat infection. Recently, we have shown the involvement of miRNAs as an adaptive strategy of <em>P. brasiliensis</em> upon metal deprivation such as iron and zinc. However, their role in copper limitation still needs to be elucidated. Our objective was to characterize the expression profile of miRNAs regulated during copper deprivation in <em>P. brasiliensis</em> and the putative altered processes. Through RNAseq analysis and bioinformatics, we identified 14 differentially expressed miRNAs, two of which putatively regulated oxidative stress response, beta-oxidation, glyoxylate cycle, and cell wall remodeling. Our results suggest that metabolic adaptations carried out by <em>P</em>. <em>brasiliensis</em> in copper deprivation are regulated by miRNAs.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105435"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Schistosoma heamatobium tetraspanins TSP-2 and TSP-6 induce Dendritic Cells maturation, cytokine production and T helper cells differentiation in vitro","authors":"Angela Silvano ,&nbsp;Javier Sotillo ,&nbsp;Marta Cecchi ,&nbsp;Alex Loukas ,&nbsp;Mireille Ouedraogo ,&nbsp;Astrid Parenti ,&nbsp;Fabrizio Bruschi ,&nbsp;Maria Gabriella Torcia ,&nbsp;Valentina D Mangano","doi":"10.1016/j.micinf.2024.105439","DOIUrl":"10.1016/j.micinf.2024.105439","url":null,"abstract":"<div><div>Urogenital schistosomiasis caused by <em>Schistosoma haematobium</em> is a major cause of disability in endemic areas. Despite its socio-economic burden, no vaccine exists and the parasite's immunobiology remains underexplored. Genome annotation has revealed over 40 different genes encoding tetraspanins, transmembrane proteins with known immunomodulatory properties in other plathelminthes. This study investigated the role of <em>Sh</em>-TSP-2, <em>Sh</em>-TSP-6 and <em>Sh</em>-TSP-23, which are expressed in the parasite's tegument and extracellular vesicles (EVs). Immature dendritic cells (DCs) from unexposed healthy donors were stimulated with these proteins to evaluate maturation maker expression and cytokine production. Also, pre-activated T CD4⁺ cells were stimulated with the DCs supernatant to assess cytokine gene expression. <em>Sh</em>-TSP-2 and <em>Sh</em>-TSP-6 induced maturation markers and cytokine production in DCs: <em>Sh</em>-TSP-2 increased CD80 and CD83 levels and the concentration of both pro-inflammatory (IL-6, TNF) and regulatory (IL-10) cytokines, while <em>Sh</em>-TSP-6 increased the production of IL-6. Moreover, supernatants from <em>Sh</em>-TSP-2 stimulated DCs induced the expression of Th1 (IFNɣ) and regulatory (IL-10) cytokines in CD4⁺ T cells, while <em>Sh-</em>TSP-6 induced Th2 (IL-4, IL-13) cytokine expression. These results provide evidence that <em>S. haematobium</em> tetraspanins modulate the response of human DCs and CD4⁺ T cells <em>in vitro,</em> and support <em>Sh</em>-TSP-2 as a promising vaccine candidate.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105439"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of anti-tuberculosis treatment on respiratory tract microbiome in pulmonary tuberculosis","authors":"Druti Hazra ,&nbsp;Kiran Chawla ,&nbsp;Fayaz S.M. ,&nbsp;Vitali Sintchenko ,&nbsp;Rahul Magazine ,&nbsp;Elena Martinez ,&nbsp;Akhilesh Pandey","doi":"10.1016/j.micinf.2024.105432","DOIUrl":"10.1016/j.micinf.2024.105432","url":null,"abstract":"<div><div>The growing evidence has underscored the significance of interactions between the host and microbiota in respiratory health, presenting a novel perspective on disease management. Yet, comprehension of the respiratory microbiome shifts before and after anti-tuberculosis treatment is limited. This study compares respiratory microbiome profiles in untreated tuberculosis (UTB) and completed TB treatment (CTB) cases with healthy controls, using 16S rRNA sequencing on sputum samples. Significant reduction in sputum microbial alpha diversity was observed in both TB groups when compared to healthy controls (P &lt; 0.05). Beta diversity analysis showed distinct clustering (P &lt; 0.05). Linear discriminant analysis revealed an abundance of potentially pathogenic bacterial genera like <em>Haemophilus, Pseudomonas</em>, and <em>Mycobacterium</em> in the UTB group, while <em>Streptococcus, Rothia</em>, and <em>Neisseria</em> dominated in CTB samples. Healthy sputum microbiomes were enriched with <em>Prevotella, Fusobacterium, Porphyromonadaceae_unclassified,</em> <em>and</em> <em>Peptostreptococcus</em>. Moreover, predicted bacterial functional pathways showed significant differences among the three groups, mainly related to nutrient metabolism.</div><div>These findings indicated significant microbial dysbiosis in sputum samples recovered from patients with pulmonary TB with an elevated presence of potentially pathogenic bacteria, depletion of beneficial genera, and downregulation of several essential metabolic pathways. Further exploration of respiratory microbiome-based diagnostic biomarkers and their role in targeted treatment strategies in tuberculosis is warranted.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105432"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution and zoonotic risk of O1:K1 and O2:K1 avian pathogenic Escherichia coli","authors":"Eun-Jin Ha ,&nbsp;Seung-Min Hong ,&nbsp;Kang-Seuk Choi ,&nbsp;Hyuk-Joon Kwon","doi":"10.1016/j.micinf.2024.105462","DOIUrl":"10.1016/j.micinf.2024.105462","url":null,"abstract":"<div><div>The O1 and O2 serogroups of avian pathogenic <em>E. coli</em> (APEC) and human extraintestinal pathogenic <em>E. coli</em> (huExPEC) are closely related, but their evolutionary relationships need to be further elucidated. This study classified nineteen O1 and O2 APEC into <em>rpoB</em> sequence types (RSTs) and compared them with reference huExPEC using molecular prophage typing, virulence and antibiotic resistance gene profiling, and comparative genomics. Most O1:K1 and O2:K1 APEC (73.7 %) were classified as RST46-1 and RST47-9. RST47-9 is unique to Korean O1 APEC and likely derives from RST46-1 APEC. The six APEC showed high genome coverage/identity with the Korean RST46-1 huExPEC. Based on RST network and comparative genomics, we hypothesized that the O1 antigen first appeared in RST19-1 and O2 in RST24-1 <em>E. coli</em> in humans. Then, O1 and O2-antigen horizontally transferred to human RST46-1, where a unique K1 capsule (K1-<em>cps</em>) first appeared. The Korean APEC and huExPEC share evolutionary CRISPR spacers but differ in molecular antibiograms and prophage contents. Thus, RST46-1 huExPEC transmitted and evolved in poultry. The zoonotic risks remain unknown, but the substantial virulence of the RST46-1 APEC indicates that the reverse zoonotic risk of huExPEC in poultry is alarming.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105462"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gingivitis- and periodontitis-associated microbiota in bovine deciduous incisor teeth – A preliminary study","authors":"Juliana Vaccari ,&nbsp;Ana C. Borsanelli ,&nbsp;Flávia R.F. Athayde ,&nbsp;Júlia R. Saraiva ,&nbsp;Thamiris N.M. Ramos ,&nbsp;Iveraldo S. Dutra","doi":"10.1016/j.micinf.2024.105468","DOIUrl":"10.1016/j.micinf.2024.105468","url":null,"abstract":"<div><div>As ruminants are frequently affected by periodontal diseases, understanding their microbial communities is crucial. In this pilot study, we analyzed subgingival biofilm samples of young cattle across different states: clinically healthy (n = 5), gingivitis (n = 5), and periodontitis (n = 5) using 16S rRNA gene sequencing and co-occurrence network analysis. The findings revealed that Proteobacteria was the predominant phylum across all conditions, with Fusobacteriota constituting 27.6 % of the microbiota in periodontitis-affected sites. In healthy sites, <em>Moraxella</em> (21.11 %), <em>Neisseria</em> (13.16 %), and <em>Lautropia</em> (7.69 %) were the predominant genera; in gingivitis-affected sites, the leading genera were <em>Neisseria</em> (23.65 %), <em>Moraxella</em> (18.95 %), and <em>Conchiformibius</em> (10.79 %); and in periodontitis sites, <em>Caviibacter</em> (19.78 %), <em>Moraxella</em> (16.13 %), and <em>Fusobacterium</em> (7.56 %) were most prevalent. Richness and dissimilarity analyses did not show significant differences across the clinical states, but differences were found between gingivitis and periodontitis sites (p = 0.01) in diversity. The co-occurrence networks highlighted significant variances in the central phyla across the phenotypes, with a higher number of positive interactions observed in periodontitis-affected sites. Consequently, this study demonstrated that the microbiota associated with periodontitis in young cattle exhibits greater diversity compared to gingivitis. Notably, in the deciduous dentition of cattle, the genera <em>Caviibacter</em> and <em>Moraxella</em> are pivotal in the context of periodontitis and periodontal health, respectively.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105468"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142915278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and in silico characterization of prophages in Helicobacter pylori clinical strains","authors":"Rute Ferreira ,&nbsp;Graça Pinto ,&nbsp;Eva Presa ,&nbsp;Mónica Oleastro ,&nbsp;Catarina Silva ,&nbsp;Luís Vieira ,&nbsp;Cláudia Sousa ,&nbsp;Diana P. Pires ,&nbsp;Ceu Figueiredo ,&nbsp;Luís D.R. Melo","doi":"10.1016/j.micinf.2024.105429","DOIUrl":"10.1016/j.micinf.2024.105429","url":null,"abstract":"<div><div>The increase of antibiotic resistance calls for alternatives to control <em>Helicobacter pylori</em>, a Gram-negative bacterium associated with various gastric diseases. Bacteriophages (phages) can be highly effective in the treatment of pathogenic bacteria. Here, we developed a method to identify prophages in <em>H. pylori</em> genomes aiming at their future use in therapy. A polymerase chain reaction (PCR)-based technique tested five primer pairs on 74 clinical <em>H. pylori</em> strains. After the PCR screening, 14 strains most likely to carry prophages were fully sequenced. After that, a more holistic approach was taken by studying the complete genome of the strains. This study allowed us to identify 12 intact prophage sequences, which were then characterized concerning their morphology, virulence, and antibiotic-resistance genes. To understand the variability of prophages, a phylogenetic analysis using the sequences of all <em>H. pylori</em> phages reported to date was performed. Overall, we increased the efficiency of identifying complete prophages to 54.1 %. Genes with homology to potential virulence factors were identified in some new prophages. Phylogenetic analysis revealed a close relationship among <em>H. pylori</em>-phages, although there are phages with different geographical origins. This study provides a deeper understanding of <em>H. pylori</em>-phages, providing valuable insights into their potential use in therapy.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105429"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bad company? The pericardium microbiome in people investigated for tuberculous pericarditis in an HIV-prevalent setting","authors":"Georgina Nyawo ,&nbsp;Charissa C. Naidoo ,&nbsp;Benjamin G. Wu ,&nbsp;Benjamin Kwok ,&nbsp;Jose C. Clemente ,&nbsp;Yonghua Li ,&nbsp;Stephanie Minnies ,&nbsp;Byron Reeve ,&nbsp;Suventha Moodley ,&nbsp;Thadathilankal-Jess John ,&nbsp;Sumanth Karamchand ,&nbsp;Shivani Singh ,&nbsp;Alfonso Pecararo ,&nbsp;Anton Doubell ,&nbsp;Charles Kyriakakis ,&nbsp;Robin Warren ,&nbsp;Leopoldo N. Segal ,&nbsp;Grant Theron","doi":"10.1016/j.micinf.2024.105434","DOIUrl":"10.1016/j.micinf.2024.105434","url":null,"abstract":"<div><h3>Background</h3><div>The site-of-disease microbiome and predicted metagenome were evaluated in a cross-sectional study involving people with presumptive tuberculous pericarditis. We also explored the interaction between C-reactive protein (CRP) and the microbiome.</div></div><div><h3>Methods</h3><div>People with effusions requiring diagnostic pericardiocentesis (n=139) provided pericardial fluid for sequencing and blood for CRP measurement.</div></div><div><h3>Results</h3><div>Pericardial fluid microbiota differed in β-diversity among people with definite (dTB, n=91), probable (pTB, n=25), and non- (nTB, n=23) tuberculous pericarditis. dTBs were <em>Mycobacterium-, Lacticigenium-,</em> and <em>Kocuria-</em>enriched vs. nTBs. HIV-positive dTBs were <em>Mycobacterium-, Bifidobacterium-</em>, <em>Methylobacterium-</em>, and <em>Leptothrix</em>-enriched vs. HIV-negative dTBs. HIV-positive dTBs on ART were <em>Mycobacterium</em>- and <em>Bifidobacterium</em>-depleted vs. those not on ART. dTBs exhibited enrichment in short-chain fatty acid (SCFA) and mycobacterial metabolism pathways vs. nTBs. Additional non-pericardial involvement (pulmonary infiltrates) was associated with <em>Mycobacterium</em>-enrichment and <em>Streptococcus</em>-depletion. <em>Mycobacterium</em> reads were in 34 % (31/91) of dTBs, 8 % (2/25) of pTBs and 17 % (4/23) nTBs. People with CRP above (vs. below) the median value had different β-diversity (<em>Pseudomonas</em>-depleted). No correlation was found between enriched taxa in dTBs and CRP.</div></div><div><h3>Conclusions</h3><div>Pericardial fluid microbial composition varies by tuberculosis status, HIV (and ART) status and dTBs are enriched in SCFA-associated taxa. The clinical significance, including mycobacterial reads in nTBs and pTBs, requires evaluation.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105434"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}