Microbes and Infection最新文献_第3页

TGEV nonstructural protein ORF3b upregulates the expression of SLA-DR at the transcriptional level in monocyte-derived porcine dendritic cells TGEV非结构蛋白ORF3b可在转录水平上上调单核细胞衍生猪树突状细胞中SLA-DR的表达。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105437

Hang Liu , Mengyao Ma , Xinhao Jia , Mengwei Qian , Bo Pang , Muzi Li , Honglei Zhang , Shijie Ma , Lanlan Zheng

{"title":"TGEV nonstructural protein ORF3b upregulates the expression of SLA-DR at the transcriptional level in monocyte-derived porcine dendritic cells","authors":"Hang Liu , Mengyao Ma , Xinhao Jia , Mengwei Qian , Bo Pang , Muzi Li , Honglei Zhang , Shijie Ma , Lanlan Zheng","doi":"10.1016/j.micinf.2024.105437","DOIUrl":"10.1016/j.micinf.2024.105437","url":null,"abstract":"<div><div>Transmissible gastroenteritis virus (TGEV) is a porcine intestinal pathogenic coronavirus that can cause acute intestinal diseases in pigs, especially in suckling piglets under two weeks of age, with a mortality rate of 100 %. Dendritic cells (DCs) are important antigen-presenting cells (APCs) that are essential for the initiation and modulation of immune responses in animals. In this study, we used monocyte-derived porcine DCs as an in vitro model of APCs to further study the pathogenic mechanism of TGEV. Our results demonstrated that TGEV successfully replicates in monocyte-derived porcine DCs, whereas UV-inactivated TGEV failed to infect these cells. Importantly, TGEV infection of DCs led to significant upregulation of swine leukocyte antigen II DR (SLA-DR), a key molecule in the major histocompatibility complex class II (MHC-II) family. We further demonstrated that the ORF3b nonstructural protein of TGEV significantly enhances SLA-DR expression at the transcriptional level in porcine DCs. This study provides new insights into the pathogenic mechanisms of TGEV.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105437"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142624011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The SARS-CoV-2 antibody-dependent enhancement façade SARS-CoV-2抗体依赖性增强实验

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105464

Jeremia M. Coish , Lori A. MacNeil , Adam J. MacNeil

{"title":"The SARS-CoV-2 antibody-dependent enhancement façade","authors":"Jeremia M. Coish , Lori A. MacNeil , Adam J. MacNeil","doi":"10.1016/j.micinf.2024.105464","DOIUrl":"10.1016/j.micinf.2024.105464","url":null,"abstract":"<div><div>Antibody-dependent enhancement (ADE) is an immunological paradox whereby sensitization following a primary viral infection results in the subsequent enhancement of a similar secondary infection. This idiosyncratic immune response has been established in dengue virus infections, driven by four antigenically related serotypes co-circulating in endemic regions. Several coronaviruses exhibit antibody-mediated mechanisms of viral entry, which has led to speculation of an ADE capacity for SARS-CoV-2, though <em>in vivo</em> and epidemiological evidence do not currently support this phenomenon. Three distinct antibody-dependent mechanisms for SARS-CoV-2 entry have recently been demonstrated: 1. FcR-dependent, 2. ACE2-FcR-interdependent, and 3. FcR-independent. These mechanisms of viral entry may be dependent on SARS-CoV-2 antibody specificity; antibodies targeting the receptor binding domain (RBD) typically result in Fc-dependent and ACE2-FcR-interdependent entry, whereas antibodies targeting the N-terminal domain can induce a conformational change to the RBD that optimizes ACE2-receptor binding domain interactions independent of Fc receptors. Whether these antibody-dependent entry mechanisms of SARS-CoV-2 result in the generation of infectious progenies and enhancement of infection has not been robustly demonstrated. Furthermore, ADE of SARS-CoV-2 mediated by antigenic seniority remains a theoretical concern, as no evidence suggests that SARS-CoV-2 imprinting blunts a subsequent immune response, contributing to severe COVID-19 disease.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105464"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting the concept of giant viruses 重新审视巨型病毒的概念。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105467

Jônatas Santos Abrahão

引用次数: 0

Proteolytic activity of secreted proteases from pathogenic leptospires and effects on phagocytosis by murine macrophages 致病性钩端螺旋体分泌蛋白酶的蛋白水解活性及其对小鼠巨噬细胞吞噬的影响。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2025.105469

Thais A. Amamura , Daniella dos S. Courrol , Angela S. Barbosa , Ildefonso A. Silva-Junior , Tiago F. da Silva , Leonardo M. Midon , Mario C. Cruz , Marcos B. Heinemann , Rosa M. Chura-Chambi , Ligia Morganti , Lourdes Isaac

{"title":"Proteolytic activity of secreted proteases from pathogenic leptospires and effects on phagocytosis by murine macrophages","authors":"Thais A. Amamura , Daniella dos S. Courrol , Angela S. Barbosa , Ildefonso A. Silva-Junior , Tiago F. da Silva , Leonardo M. Midon , Mario C. Cruz , Marcos B. Heinemann , Rosa M. Chura-Chambi , Ligia Morganti , Lourdes Isaac","doi":"10.1016/j.micinf.2025.105469","DOIUrl":"10.1016/j.micinf.2025.105469","url":null,"abstract":"<div><div>Leptospirosis is a zoonosis caused by spirochete <em>Leptospira.</em> Pathogenic leptospires evade the Complement System, enabling their survival upon contact with normal human serum <em>in vitro</em>. In a previous study, we demonstrated that proteases secreted by pathogenic leptospires cleave several Complement proteins, including C3 and the opsonins C3b and iC3b. We hypothesize that these <em>Leptospira</em> proteases, such as thermolysin and leptolysin, may decrease the phagocytic activity of murine peritoneal macrophages. We observed decreased amounts of CR3 and CR4 using flow cytometry when these cells were treated with supernatant from the culture of pathogenic leptospires (SPL) for 24 h. Through confocal microscopy, we observed a reduction in TLR2, CD11b, and CD206 (mannose receptor) levels when these cells were treated with SPL or recombinant thermolysin for 24 h. Furthermore, opsonins such as C3b/iC3b deposited on the surface of pathogenic leptospires were clearly degraded in the presence of recombinant thermolysin or recombinant leptolysin. Consequently, when opsonized bacteria and macrophages were previously incubated with these proteases, phagocytic activity was diminished. These observations lead us to suggest that proteases secreted by pathogenic leptospires could degrade opsonins present in normal serum or deposited on the bacterial membrane, as well as cleave or inhibit macrophage surface molecules. Therefore, these proteases could interfere with the recognition and internalization by murine macrophages, favoring the spread of leptospires in the host.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105469"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142951207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Obituary Tomasz Skirecki (May 22nd, 1987–January 21st, 2025) 托马兹·斯基雷基讣告（1987年5月22日- 2025年1月21日）

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2025.105482

Marcin Osuchowski, Jean-Marc Cavaillon

引用次数: 0

Dopaminergic neuronal regulation determines innate immunity of Caenorhabditis elegans during Klebsiella aerogenes infection 多巴胺能神经元调控决定了草履虫在产气克雷伯氏菌感染期间的先天免疫力。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105430

Gowripriya Thirumugam , Yashwanth Radhakrishnan , James Prabhanand Bhaskar , Suresh Ramamurthi , Balamurugan Krishnaswamy

{"title":"Dopaminergic neuronal regulation determines innate immunity of Caenorhabditis elegans during Klebsiella aerogenes infection","authors":"Gowripriya Thirumugam , Yashwanth Radhakrishnan , James Prabhanand Bhaskar , Suresh Ramamurthi , Balamurugan Krishnaswamy","doi":"10.1016/j.micinf.2024.105430","DOIUrl":"10.1016/j.micinf.2024.105430","url":null,"abstract":"<div><div>The innate immune signals are the front line of host defense against bacterial pathogens. Pathogen-induced harmful effects, such as reduced neuronal signals to the intestine, affect the host's food sensing and dwelling behavior. Here, we report that dopamine and <em>kpc-1</em> signals control the intestinal innate immune responses through the p38/PMK-1 MAPK signaling pathway in <em>C. elegans</em>. <em>K. aerogenes</em> infection in <em>C. elegans</em> affects the food-dwelling behavior, which depends on dopamine regulation. The absence of the dopamine receptor (<em>dop-1</em>) and transporter (<em>dat-1</em>) increases attraction to the pathogen instead of avoidance. The <em>K. aerogenes</em> infection affects <em>age-1</em> regulation through the furin-like proprotein convertase (<em>kpc-1</em>); the absence of <em>kpc-1</em> affects environment-dependent dauer formation. In contrast, the <em>dop-1</em> mutation antagonistically regulates intestinal immune regulation, while the <em>kpc-1</em> mutation partially regulates the p38/PMK-1 MAPK pathway. Our findings indicate that dopamine and <em>kpc-1</em>signaling from the nervous system control intestinal immunity in an antagonistic and agonistic manner, respectively.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105430"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The efficacy of the bacteriocinogenic Enterococcus faecalis 14 in the control of induced necrotic enteritis in broilers 产菌性粪肠球菌14对肉鸡诱导性坏死性肠炎的防治效果。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2025.105477

Rabia Ladjouzi , Bernard Taminiau , Georges Daube , Anca Lucau-Danila , Djamel Drider

{"title":"The efficacy of the bacteriocinogenic Enterococcus faecalis 14 in the control of induced necrotic enteritis in broilers","authors":"Rabia Ladjouzi , Bernard Taminiau , Georges Daube , Anca Lucau-Danila , Djamel Drider","doi":"10.1016/j.micinf.2025.105477","DOIUrl":"10.1016/j.micinf.2025.105477","url":null,"abstract":"<div><h3>Purpose</h3><div>To demonstrate the efficacy of the bacteriocinogenic <em>Enterococcus faecalis</em> 14 (<em>E. faecalis</em> 14) in the control of induced necrotic enteritis (NE) in broilers.</div></div><div><h3>Methods</h3><div>Six groups of 504 broilers consisting of an infected untreated control (IUC) group, an infected and amoxicillin treated control (ITC) group, and groups receiving prophylactically (2 groups) or therapeutically (2 groups) <em>E. faecalis</em> 14 or its Δ<em>bac</em> mutant were used. All groups were challenged with <em>Clostridium perfringens</em> 56 to induce NE. To predispose the boilers to develop subclinical NE, a high protein grower diet containing 15 % fishmeal and a coccidial inoculum were administered.</div></div><div><h3>Results</h3><div>NE lesions were observed on D26 in all groups except ITC and those receiving prophylactically and therapeutically <em>E. faecalis</em> 14. On D27, only ITC and the group prophylactically treated with <em>E. faecalis</em> 14 (T03) were without lesions. Average body weight and daily weight gain remained lower in the treated groups compared to the ITC group, but there was a clear improvement in the period between D21 to D27, especially in the group prophylactically treated with <em>E. faecalis</em> 14. Specifically, the daily weight gain (DWG) in this period for group T03, was second highest after the group ITC. Metataxonomic analyses showed a positive effect of <em>E. faecalis</em> 14 in maintaining the diversity and richness of the intestinal microbiota, in contrast to ITC group and other conditions.</div></div><div><h3>Conclusions</h3><div>The results of this <em>in vivo</em> study demonstrated the efficacy of the prophylactic administration of the bacteriocinogenic <em>E. faecalis</em> 14 in preventing of the NE lesions caused by <em>C. perfringens</em>.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105477"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chytridiomycosis disrupts metabolic responses in amphibians at metamorphic climax 恙虫病扰乱了处于变态高潮期的两栖动物的新陈代谢反应。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105438

Josephine E. Humphries , Steven D. Melvin , Chantal Lanctôt , Hamish McCallum , David Newell , Laura F. Grogan

{"title":"Chytridiomycosis disrupts metabolic responses in amphibians at metamorphic climax","authors":"Josephine E. Humphries , Steven D. Melvin , Chantal Lanctôt , Hamish McCallum , David Newell , Laura F. Grogan","doi":"10.1016/j.micinf.2024.105438","DOIUrl":"10.1016/j.micinf.2024.105438","url":null,"abstract":"<div><div>The fungal disease chytridiomycosis (causative agent <em>Batrachochytrium dendrobatidis</em> [Bd]) is a primary contributor to amphibian species declines. The morphological and physiological reorganization that occurs during amphibian metamorphosis likely increases the vulnerability of metamorphs to Bd. To address this, we exposed pro-metamorphic tadpoles of Fleay's barred frog (<em>Mixophyes fleayi)</em> to Bd and sampled skin and liver sections from control and exposed animals throughout metamorphosis (Gosner stages 40, 42 and 45). We used an untargeted metabolomics approach to assess the metabolic impacts of Bd infection during the critical metamorphic stages, extracting metabolites from sampled tissues and analysing them via Nuclear Magnetic Resonance spectrometry. Most exposed animals became moribund at Gosner stage 45, while a subset seemingly cleared their infections. Metabolite abundance varied throughout development, with Gosner stage 45 samples distinct from previous stages. Clinically infected animals at Gosner stage 45 exhibited profound metabolic dysregulation (e.g., upregulation of amino acid biosynthesis and degradation) in comparison to uninfected groups (negative controls and ‘cleared’ animals). Despite showing parallels with previous metabolomic analyses of Bd-infected adult frogs, we identified variations in our results that could be attributed to the dramatic changes that characterise metamorphosis and may be driving the heightened vulnerability observed in metamorphic amphibians.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105438"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of route of delivery on Chlamydia abortus vaccine-induced immune responses and genital tract immunity in mice 递送途径对流产衣原体疫苗诱导的免疫反应和小鼠生殖道免疫的作用。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-03-01 DOI: 10.1016/j.micinf.2024.105463

Shakyra Richardson , F.N.U. Medhavi , Tayhlor Tanner , Stephanie Lundy , Yusuf Omosun , Joseph U. Igietseme , Francis O. Eko

{"title":"Role of route of delivery on Chlamydia abortus vaccine-induced immune responses and genital tract immunity in mice","authors":"Shakyra Richardson , F.N.U. Medhavi , Tayhlor Tanner , Stephanie Lundy , Yusuf Omosun , Joseph U. Igietseme , Francis O. Eko","doi":"10.1016/j.micinf.2024.105463","DOIUrl":"10.1016/j.micinf.2024.105463","url":null,"abstract":"<div><div>We investigated if the efficacy of a <em>Chlamydia abortus</em> (Cab) subunit vaccine is influenced by route of administration. Thus, female CBA/J mice were immunized either by mucosal or systemic routes with <em>Vibrio cholerae</em> ghost (VCG)-based vaccine expressing T and B cell epitopes of Cab polymorphic membrane protein (Pmp) 18D, termed rVCG-Pmp18.3. Vaccine evaluation revealed that all routes of vaccine delivery induced a Th1-type antibody response after a prime boost or three-dose immunization regimen. Also, the intranasal and rectal mucosal and intramuscular systemic routes induced cross-reactive neutralizing antibodies against homologous and heterologous Cab strains. Irrespective of the route of immunization, the vaccine elicited a Th1-type cytokine response (IFN-γ/IL-4 >1) in immunized mice. Analysis of reduction in genital Cab burden as an index of protection showed that immunization induced substantial degrees of protection against infection, irrespective of route of delivery with the intranasal and rectal mucosal routes showing superior levels of protection 12 days postchallenge. Furthermore, there was correlation between the humoral and cellular immune response and protection was associated with the Cab-specific serum IgG antibody avidity and IFN-γ. Thus, while route of administration impacts vaccine efficacy, the rVCG-Pmp18.3-induced protective immunity against Cab respiratory infection can be accomplished by both mucosal and systemic immunization.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 3","pages":"Article 105463"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas aeruginosa-derived DnaJ induces TLR2 expression through TLR10-mediated activation of the PI3K-SGK1 pathway in macrophages. 铜绿假单胞菌衍生的DnaJ通过tlr10介导的巨噬细胞PI3K-SGK1通路激活诱导TLR2表达。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-02-18 DOI: 10.1016/j.micinf.2025.105481

Jaehoo Lee, Yongxin Jin, Weihui Wu, Yeji Lee, Un-Hwan Ha

{"title":"Pseudomonas aeruginosa-derived DnaJ induces TLR2 expression through TLR10-mediated activation of the PI3K-SGK1 pathway in macrophages.","authors":"Jaehoo Lee, Yongxin Jin, Weihui Wu, Yeji Lee, Un-Hwan Ha","doi":"10.1016/j.micinf.2025.105481","DOIUrl":"10.1016/j.micinf.2025.105481","url":null,"abstract":"<p><p>TLR2 is a key component of the innate immune system, responsible for recognizing Gram-positive bacterial components and initiating inflammatory signaling cascades that activate defense responses. However, little is known about the regulatory effects of Pseudomonas aeruginosa (P. aeruginosa) on TLR2 expression. In this study, we investigated the potential link between P. aeruginosa-derived DnaJ and TLR2 expression in macrophages, as well as the activation of downstream signaling pathways. Our findings revealed that DnaJ significantly induced TLR2 expression in a dose- and time-dependent manner, predominantly affecting TLR2 with minimal impact on other TLRs, such as TLR4 and TLR5, which detect bacterial PAMPs. The DnaJ-mediated TLR2 induction was driven by activation of the PI3K-SGK1 signaling pathway, with TLR10 playing a crucial role in facilitating these effects. This increase in TLR2 expression led to enhanced production of inflammatory cytokines in response to secondary Staphylococcus aureus infections, indicating a role in boosting host defense mechanisms. In conclusion, these findings suggest that P. aeruginosa-derived DnaJ promotes TLR2 expression via TLR10-mediated activation of the PI3K-SGK1 pathway, thereby enhancing host immune responses against Gram-positive bacterial infections.</p>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105481"},"PeriodicalIF":2.6,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0