Microbes and Infection最新文献_第10页

NLRP1B allele 2 does not respond to Val-boro-Pro (VbP) in intestinal epithelial cells 肠上皮细胞中表达的 NLRP1B 对 Val-boro-Pro 的激活具有耐受性。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105398

Ryan J. Mazzone , Nathaniel J. Winsor , Lu Yi Li , Kristian T. Barry , Adrienne Ranger , Shawn Goyal , Justin J. Meade , Jessica Bruce , Dana J. Philpott , Jeremy Mogridge , Stephen E. Girardin

{"title":"NLRP1B allele 2 does not respond to Val-boro-Pro (VbP) in intestinal epithelial cells","authors":"Ryan J. Mazzone , Nathaniel J. Winsor , Lu Yi Li , Kristian T. Barry , Adrienne Ranger , Shawn Goyal , Justin J. Meade , Jessica Bruce , Dana J. Philpott , Jeremy Mogridge , Stephen E. Girardin","doi":"10.1016/j.micinf.2024.105398","DOIUrl":"10.1016/j.micinf.2024.105398","url":null,"abstract":"<div><div>The intestinal mucosa must balance tolerance to commensal microbes and luminal antigens with rapid detection of enteric pathogens in order to maintain homeostasis. This balance is facilitated through the regulation of epithelial layer integrity by innate immune receptors. Certain NOD-like receptors (NLRs) expressed in intestinal epithelial cells, including NLRC4 and NLRP9B, form inflammasomes that protect against pathogens by activating caspase-1 to cause extrusion of infected cells. NLRP1B is a murine NLR encoded by five alleles of a highly polymorphic gene homologous to human NLRP1. NLRP1B forms inflammasomes in response to a variety of pathogens that cause intestinal infections, but it has almost exclusively been studied in immune cells and has not been characterized in cells of the intestinal epithelium. Here, we show that <em>Nlrp1b</em> allele 2 is expressed in ileal and colonic organoids derived for C57BL/6J mice, while the related gene <em>Nlrp1a</em> was not expressed. <em>Nlrp1b</em> was upregulated by interleukin-13 in organoids and by the protozoan <em>Tritrichomonas muris</em> in vivo, suggesting that NLRP1B may be involved in defense against enteric parasites. Surprisingly, while Val-boro-Pro (VbP) activated C57BL/6J-derived bone marrow-derived macrophages, which expressed both <em>Nlrp1a</em> and <em>Nlrp1b</em>, it did not activate intestinal organoids of the same genotype. We furthermore did not detect <em>Nlrp1b</em> in organoids derived from Balb/cJ mice, which express a different allele than the one expressed in C57BL/6J mice. Together, our results suggest that NLRP1B may have an allele-dependent function in murine IECs whose regulation is distinct from that of macrophages, and that the response to VbP might be exclusively driven by NLRP1A in C57BL/6J mice.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105398"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141759697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Factors underlying the long-term efficacy of faecal microbiota transplantation for patients with irritable bowel syndrome 粪便微生物群移植对肠易激综合征患者的长期疗效的基础因素。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105372

Magdy El-Salhy , Odd Helge Gilja , Jan Gunnar Hatlebakk

{"title":"Factors underlying the long-term efficacy of faecal microbiota transplantation for patients with irritable bowel syndrome","authors":"Magdy El-Salhy , Odd Helge Gilja , Jan Gunnar Hatlebakk","doi":"10.1016/j.micinf.2024.105372","DOIUrl":"10.1016/j.micinf.2024.105372","url":null,"abstract":"<div><div>The long-term effects of the transplant dose, its administration route and repeated faecal microbiota transplantation (FMT) on the outcomes of FMT for patients with irritable bowel syndrome (IBS) are unknown. This study included 171 patients (125 females and 46 males): 90 g of donor feces was administered into the large intestine (LI) in 58, into the small intestine (SI) in 57, and into the SI twice (repeated SI) in 56. The patients provided a fecal sample and completed five questionnaires at the baseline and at 2 years after FMT. Fecal bacteria and the dysbiosis index were analyzed using 16S rRNA gene PCR DNA amplification/probe. The response rates at 2 years after FMT were 47.2%, 80.9%, and 76.6% in the LI, SI, and repeated-SI groups, respectively. The response rate was significantly higher in the SI and repeated SI groups than in the LI group. IBS symptoms at 2 years after FMT were less severe in the SI and repeated-SI groups than in the LI group. Fluorescent signals of several bacteria were significantly correlated with IBS symptoms and fatigue after FMT. No long-term adverse events were observed. In conclusion, administering the transplant to the SI increased the long-term response rate and reduced IBS symptom severity compared with administering it to the LI, and led to the long-term colonization of beneficial bacteria. There was no long-term difference between one and two FMT procedures (<span><span>www.clinicaltrials.gov</span><svg><path></path></svg></span>: NCT04236843).</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105372"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “N-acetyl-cysteine mediates protection against Mycobacterium avium through induction of human β-defensin-2” [Microb Infect 22 (10) (2020) 567–575] N-乙酰-半胱氨酸通过诱导人β-防御素-2介导对分枝杆菌的保护"[Microb Infect 22 (10) (2020) 567-575] 的更正。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105388

Ayako Shiozawa, Chiaki Kajiwara, Yoshikazu Ishii, Kazuhiro Tateda

引用次数: 0

Leishmania donovani modulates host miRNAs regulating cholesterol biosynthesis for its survival 唐氏利什曼原虫调节宿主的 miRNA，调控胆固醇的生物合成，以促进其生存

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105379

Shams Tabrez , Sajjadul Kadir Akand , Rahat Ali , Irshad Husain Naqvi , Neha Soleja , Mohd Mohsin , Mohammad Z. Ahmed , Mohammed Saleem , Suhel Parvez , Yusuf Akhter , Abdur Rub

{"title":"Leishmania donovani modulates host miRNAs regulating cholesterol biosynthesis for its survival","authors":"Shams Tabrez , Sajjadul Kadir Akand , Rahat Ali , Irshad Husain Naqvi , Neha Soleja , Mohd Mohsin , Mohammad Z. Ahmed , Mohammed Saleem , Suhel Parvez , Yusuf Akhter , Abdur Rub","doi":"10.1016/j.micinf.2024.105379","DOIUrl":"10.1016/j.micinf.2024.105379","url":null,"abstract":"<div><div><span>Cholesterol reduction by intracellular protozoan parasite </span><span><span>Leishmania donovani</span></span> (<em>L. donovani</em>)<em>,</em><span><span> causative agent of leishmaniasis, impairs </span>antigen presentation<span>, pro-inflammatory cytokine secretion<span> and host-protective membrane-receptor signaling in macrophages. Here, we studied the miRNA mediated regulation of cholesterol biosynthetic genes to understand the possible mechanism of </span></span></span><em>L. donovani-</em><span><span>induced cholesterol reduction and therapeutic importance of miRNAs in leishmaniasis. System-scale genome-wide microtranscriptome screening was performed to identify the miRNAs involved in the regulation of expression of key </span>cholesterol biosynthesis regulatory genes through miRanda3.0. 11 miRNAs out of 2823, showing complementarity with cholesterol biosynthetic genes were finally selected for expression analysis. These selected miRNAs were differentially regulated in THP-1 derived macrophages and in primary human macrophages by </span><em>L. donovani</em><span>. Correlation of expression and target validation through luciferase assay suggested two key miRNAs, hsa-miR-1303 and hsa-miR-874-3p regulating the key genes </span><span><span>hmgcr</span></span> and <em>hmgcs1</em> respectively. Inhibition of hsa-mir-1303 and hsa-miR-874-3p augmented the expression of targets and reduced the parasitemia in macrophages. This study will also provide the platform for the development of miRNA-based therapy against leishmaniasis.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105379"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141398578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HERV-W ENV transcription in B cells predicting symptomatic COVID-19 and risk for long COVID can express a full-length protein despite stop codon in mRNA from chromosome X via a ribosome readthrough. HERV-W ENV 在 B 细胞中的转录可预测有症状的 COVID-19 和长 COVID 风险，尽管来自 X 染色体的 mRNA 中存在终止密码子，但 HERV-W ENV 仍可通过核糖体通读表达全长蛋白质。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-10-16 DOI: 10.1016/j.micinf.2024.105431

Joanna Brunel, Julien Paganini, Melissa Galloux, Benjamin Charvet, Hervé Perron

{"title":"HERV-W ENV transcription in B cells predicting symptomatic COVID-19 and risk for long COVID can express a full-length protein despite stop codon in mRNA from chromosome X via a ribosome readthrough.","authors":"Joanna Brunel, Julien Paganini, Melissa Galloux, Benjamin Charvet, Hervé Perron","doi":"10.1016/j.micinf.2024.105431","DOIUrl":"10.1016/j.micinf.2024.105431","url":null,"abstract":"<p><p>The human genome comprises 8 % of endogenous retroviruses (HERVs). Though HERVS contribute to physiological functions, copies retained pathogenic potential. The HERV-W ENV protein was shown expressed in patients with worse COVID-19 symptoms and post-COVID syndrome. A significant detection of the mRNA encoding HERV-W ENV from patients with COVID-19 in B cells from RNAseq reads obtained from peripheral blond mononuclear cells. This data stratified with increased COVID-19 symptoms or with post-acute sequelae of COVID-19 (long COVID) after 3 months. The HERV-W ENV-U3R RNA was confirmed to display the best alignment with chromosome X ERVWE2 locus. However, a stop codon precluding its translation was re-addressed after recent understandings of ribosome readthrough mechanisms. Experimental results evidenced that this HERV gene can effectively express a full-length protein in the presence of molecules allowing translation via a readthrough mechanism at the ribosome level. Results not only confirm HERV-W ENV RNA origin in these patients but show for the first time how a defective HERV copy can be translated into a complete protein when specific factors make it possible at the ribosome level. The present proof of concept now requires further studies to identify the factors involved in this newly understood mechanism, following SARS-CoV-2 exposure.</p>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105431"},"PeriodicalIF":2.6,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk between human endogenous retroviruses and exogenous viruses. 人类内源性逆转录病毒与外源性病毒之间的相互影响。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-09-28 DOI: 10.1016/j.micinf.2024.105427

Edoardo Pizzioli, Antonella Minutolo, Emanuela Balestrieri, Claudia Matteucci, Gkikas Magiorkinis, Branka Horvat

引用次数: 0

Copyright page Elsevier 版权页

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-09-01 DOI: 10.1016/S1286-4579(24)00159-X

引用次数: 0

Organoids and organ-on-chip technology for investigating host-microorganism interactions 用于研究宿主与微生物相互作用的有机体和芯片上有机体技术。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-09-01 DOI: 10.1016/j.micinf.2024.105319

引用次数: 0

Microbiome and infectious disease: diagnostics to therapeutics 微生物和传染性疾病：从诊断到治疗

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-09-01 DOI: 10.1016/j.micinf.2024.105345

引用次数: 0

Unraveling the intricacies of host-pathogen interaction through single-cell genomics 通过单细胞基因组学揭示宿主与病原体相互作用的复杂性。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-09-01 DOI: 10.1016/j.micinf.2024.105313

引用次数: 0