Microbes and Infection最新文献_第6页

Kinetic and proteomic studies in milk show distinct patterns among major Listeria monocytogenes clones 牛奶中的动力学和蛋白质组学研究显示，主要李斯特菌克隆之间存在不同的模式。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2024.105312

Alba Espí-Malillos , Carla Palacios-Gorba , Inmaculada López-Almela , Pilar Ruiz-García , María Carmen López-Mendoza , Francisco García-Del Portillo , M Graciela Pucciarelli , Juan J. Quereda

{"title":"Kinetic and proteomic studies in milk show distinct patterns among major Listeria monocytogenes clones","authors":"Alba Espí-Malillos , Carla Palacios-Gorba , Inmaculada López-Almela , Pilar Ruiz-García , María Carmen López-Mendoza , Francisco García-Del Portillo , M Graciela Pucciarelli , Juan J. Quereda","doi":"10.1016/j.micinf.2024.105312","DOIUrl":"10.1016/j.micinf.2024.105312","url":null,"abstract":"<div><div>Listeria monocytogenes, a contaminant of raw milk, includes hypervirulent clonal complexes (CC) like CC1, CC4, and CC6, highly overrepresented in dairy products when compared to other food types. Whether their higher prevalence in dairy products is the consequence of a growth advantage in this food remains unknown. We examined growth kinetics of five L. monocytogenes isolates (CC1, CC4, CC6, CC9, and CC121) at 37 and 4 °C in ultra-high temperature (UHT) milk and raw milk. At 4 °C, hypovirulent CC9 and CC121 isolates exhibit better growth parameters in UHT milk compared to the hypervirulent CC1, CC4, and CC6 isolates. CC9 isolate in raw milk at 4 °C exhibited the fastest growth and the highest final concentrations. In contrast, hypervirulent isolates (CC1, CC4, and CC6) displayed better growth rates in UHT milk at 37 °C, the mammalian host temperature. Proteomic analysis of representative hyper- (CC1) and hypovirulent (CC9) isolates showed that they respond to milk cues differently with CC-specific traits. Proteins related to metabolism (such as LysA or different phosphotransferase systems), and stress response were upregulated in both isolates during growth in UHT milk. Our results show that there is a Listeria CC-specific and a Listeria CC-common response to the milk environment. These findings shed light on the overrepresentation of hypervirulent L. monocytogenes isolates in dairy products, suggesting that CC1 and CC4 overrepresentation in dairy products made of raw milk may arise from contamination during or after milking at the farm and discard an advantage of hypervirulent isolates in milk products when stored at refrigeration temperatures.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105312"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139723314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dual M protein systems have diverse biological characteristics, but both contribute to M18-type Group A Streptococcus pathogenicity 双M蛋白系统具有不同的生物学特性，但两者都有助于M18型A组链球菌的致病性。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2023.105209

Xiaorui Zhou , Haoshuai Song , Fei Pan , Chen Yuan , Lu Jia , Bing Wu , Hongjie Fan , Zhe Ma

{"title":"The dual M protein systems have diverse biological characteristics, but both contribute to M18-type Group A Streptococcus pathogenicity","authors":"Xiaorui Zhou , Haoshuai Song , Fei Pan , Chen Yuan , Lu Jia , Bing Wu , Hongjie Fan , Zhe Ma","doi":"10.1016/j.micinf.2023.105209","DOIUrl":"10.1016/j.micinf.2023.105209","url":null,"abstract":"<div><div>M protein is a key surface virulence factor in Group A Streptococcus (GAS), Group C Streptococcus (GCS), and other streptococcal species. GAS encodes M protein using the emm gene, while GCS employs the szm (or sem) gene. In M18-type GAS, dual M protein systems exist, comprising both GAS and GCS M proteins (encoded separately by emm18 and spa18). The spa18 gene in M18-type GAS shares a conserved region highly similar to GCS's szm gene. Our study reveals that spa18 exhibits higher transcription levels than emm18 in M18-type GAS strains. The dual M protein systems defective mutant (Δemm18Δspa18) displays a smooth surface, whereas wild-type and single M protein gene mutants remain rough. M18 and SPA18 proteins possess distinct characteristics, showing varied binding properties and cytotoxicity effects on macrophages (THP-1) and keratinocytes (HaCaT). Both emm18 and spa18 genes contribute to the skin pathogenicity of M18-type GAS. Transcriptome analysis suggests the potential involvement of the mga gene in spa18 transcription regulation, while SpyM18_2047 appears to be specific to spa18 regulation. In summary, this research offers a crucial understanding of the biological characteristics of dual M protein systems in M18-type GAS, highlighting their contributions to virulence and transcriptional regulation.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105209"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10030163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rab27a via its effector JFC1 localizes to Anaplasma inclusions and promotes Anaplasma proliferation in leukocytes Rab27a 通过其效应物 JFC1 定位于阿那普拉斯原虫包涵体并促进阿那普拉斯原虫在白细胞中的增殖

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2023.105278

Weiyan Huang, Mingqun Lin, Yasuko Rikihisa

{"title":"Rab27a via its effector JFC1 localizes to Anaplasma inclusions and promotes Anaplasma proliferation in leukocytes","authors":"Weiyan Huang, Mingqun Lin, Yasuko Rikihisa","doi":"10.1016/j.micinf.2023.105278","DOIUrl":"10.1016/j.micinf.2023.105278","url":null,"abstract":"<div><div>Anaplasma phagocytophilum is an obligatory intracellular bacterium that causes tick-borne zoonosis called human granulocytic anaplasmosis. Mechanisms by which Anaplasma replicates inside of the membrane-bound compartment called “inclusion” in neutrophils are incompletely understood. A small GTPase Rab27a is found in the secretory granules and multivesicular endosomes. In this study we found Rab27a-containing granules were localized to Anaplasma inclusions in guanine nucleotide-dependent manner, and constitutively active Rab27a enhanced Anaplasma infection and dominant-negative Rab27a inhibited Anaplasma infection. Rab27a effector, JFC1 is known to mediate docking/fusion of Rab27a-bearing granules for exocytosis in leukocytes. shRNA stable knockdown of Rab27a or JFC1 inhibited Anaplasma infection in HL-60 cells. Similar to Rab27a, both endogenous and transfected JFC1 were localized to Anaplasma inclusions by immunostaining or live cell imaging. The JFC1 C2A domain that binds 3′-phosphoinositides, was sufficient and required for JFC1 and Rab27a localization to Anaplasma inclusions which were enriched with phosphatidylinositol 3-phosphate. Nexinhib20, the small molecule inhibitor specific to Rab27a and JFC1 binding, inhibited Anaplasma infection. Taken together, these results imply elevated phosphatidylinositol 3-phosphate in the inclusion membrane recruits JFC1 to mediate Rab27a-bearing granules/vesicles to dock/fuse with Anaplasma inclusions, the lumen of which is topologically equivalent to the exterior of the cell to benefit Anaplasma proliferation.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105278"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138682491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The functions of EF-hand proteins from host and zoonotic pathogens 宿主和人畜共患病原体的 EF-手蛋白的功能

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2023.105276

Wei Zuo , Mingxing Tian , Jingjing Qi , Guangdong Zhang , Jiangang Hu , Shaohui Wang , Yanqing Bao

引用次数: 0

Lineage classification and selective site identification of Orthoebolavirus zairense Orthoebolavirus zairense 的品系分类和选择性位点鉴定。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2024.105304

Jie Fang , Zhi-jian Zhou , Shuofeng Yuan , Ye Qiu , Xing-Yi Ge

{"title":"Lineage classification and selective site identification of Orthoebolavirus zairense","authors":"Jie Fang , Zhi-jian Zhou , Shuofeng Yuan , Ye Qiu , Xing-Yi Ge","doi":"10.1016/j.micinf.2024.105304","DOIUrl":"10.1016/j.micinf.2024.105304","url":null,"abstract":"<div><div>As the high pathogenic species of Filoviridae virus family, Orthoebolavirus zairense (EBOV) shows frequent outbreaks in human in recently years since its first emerging in 1976 in Democratic Republic of the Congo (COD), bringing ongoing risks and burden on public health safety. Here, the phylogenetic relationship among major outbreaks was analyzed. The results showed that EBOV isolates could be divided into four lineages according to spatial and temporal epidemics. Then, the positive selection sites (PSSs) were detected on all proteins of the EBOV, exhibiting lineage characteristic. Particularly, sites in GP and VP24 were identified to be significantly under positive selection, and partial of which were maintained in the latest isolates in 2021. GP and L were found to have high variability between lineages. Substitutions including F443L and F443S in GP, as well as F1610L and I1951V in L could be characteristic of the two large outbreaks in COD (2018) and West Africa (2014), respectively. Further, substitutions of significant PSSs in VP24 and L proteins were visualized for analysis of structural changes, which may affect EBOV pathogenesis. In summary, our results gains insights in genetic characteristic and adaptive evolution of EBOV, which could facilitate gene functional research against EBOV.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105304"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139567237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycerol metabolic repressor GlpR contributes to Streptococcus suis oxidative stress resistance and virulence 甘油代谢抑制因子 GlpR 对猪链球菌抗氧化压力和病毒性有贡献

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2024.105307

Zijing Liang , Jiaxuan Lu , Yinli Bao , Xiang Chen , Huochun Yao , Zongfu Wu

{"title":"Glycerol metabolic repressor GlpR contributes to Streptococcus suis oxidative stress resistance and virulence","authors":"Zijing Liang , Jiaxuan Lu , Yinli Bao , Xiang Chen , Huochun Yao , Zongfu Wu","doi":"10.1016/j.micinf.2024.105307","DOIUrl":"10.1016/j.micinf.2024.105307","url":null,"abstract":"<div><div>Bacterial DeoR family transcription regulators regulate multiple physiological processes. Little is known about the function of DeoR family regulators in streptococci. Here, we identified a novel DeoR family regulator, GlpR, from Streptococcus suis, a pathogen causing severe diseases in pigs and humans. GlpR was involved in glycerol utilization and exhibited specific signature residues at positions 30–31 (KV) which are crucial for DNA binding. Deletion of glpR (ΔglpR) showed a significant increase in relative growth rate in glycerol medium compared to the wild-type (WT) and complementary strains (CΔglpR). Employing RNA-seq analysis, β-galactosidase activity analysis, and electrophoretic mobility shift assay, we discovered that GlpR directly represses the expression of glycerol metabolism-related genes pflB2, pflA1, and fsaA, encoding pyruvate formate-lyase and its activating enzyme, and fructose-6-phosphate aldolase, respectively. Compared to WT and CΔglpR, ΔglpR showed a reduced survival rate under oxidative stress and in murine macrophages and attenuated virulence in mice. GlpR probably enhances oxidative stress resistance and virulence in S. suis by functioning as a glycerol metabolic repressor decreasing energy consumption. These findings contribute to a better understanding of S. suis pathogenesis and enrich our knowledge of the biological functions of DeoR family regulators in streptococci.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105307"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139662958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of two-component system ArcAB and the universal stress protein E in Pasteurella multocida and their effects on bacterial fitness and pathogenesis 多杀性巴氏杆菌双组分系统ArcAB和通用应激蛋白E的鉴定及其对细菌适应度和发病机制的影响。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2023.105235

Qingjie Lv , Yuyao Shang , Haixin Bi , Jie Yang , Lin Lin , Congcong Shi , Mixue Wang , Rui Xie , Zhanwei Zhu , Fei Wang , Lin Hua , Huanchun Chen , Bin Wu , Zhong Peng

{"title":"Identification of two-component system ArcAB and the universal stress protein E in Pasteurella multocida and their effects on bacterial fitness and pathogenesis","authors":"Qingjie Lv , Yuyao Shang , Haixin Bi , Jie Yang , Lin Lin , Congcong Shi , Mixue Wang , Rui Xie , Zhanwei Zhu , Fei Wang , Lin Hua , Huanchun Chen , Bin Wu , Zhong Peng","doi":"10.1016/j.micinf.2023.105235","DOIUrl":"10.1016/j.micinf.2023.105235","url":null,"abstract":"<div><div>Two-component regulatory system (TCS) is a widespread bacterial signal transduction mechanism and plays a critical role in bacterial adaptation to environments as well as regulating bacterial virulence. However, few studies have reported the actions of TCS in Pasteurella multocida, a zoonotic bacterial pathogen. In this study, genes encoding proteins homologous to the ArcAB TCS were identified in genome sequences of P. multocida belonging to different serogroups, and the transcription of both arcA and arcB was up-regulated in anaerobic and superoxygen environment. Compared to wild type strains, P. multocida arcA-deletion mutants (ΔarcA) displayed a decrease in growing under anaerobic conditions, biofilm formation, as well as the capacities of anti-serum bactericidal effect, cell adherence and invasion, anti-phagocytosis, and virulence in different in vivo models (Galleria mellonella and mice). RNA-Seq identified 70 significantly downregulated genes in ΔarcA compared to the wild type strain, and several of them are associated with P. multocida virulence. Among them, a universal stress protein E encoding gene uspE was characterized in P. multocida for the first time. Electrophoretic mobility shift assay (EMSA) demonstrated that the ArcAB TCS could regulate uspE directly. Deletion of uspE also led to a decrease of P. multocida in growing under anaerobic conditions, biofilm formation, anti-serum bactericidal effect, cell adherence and invasion, anti-phagocytosis, and virulence in mice. The data provided from this study will help further understanding the fitness and pathogenesis of P. multocida.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105235"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41132917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HERV reactivation by adenovirus infection is associated with viral immune regulation. "腺病毒感染导致的 HERV 再激活与病毒免疫调节有关"。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-12-21 DOI: 10.1016/j.micinf.2024.105466

Wen Liang, Miona Stubbe, Lisa Pleninger, Anna Hofferek, Hans Stubbe, Julia Mai, Salih Özer, Dmitrij Frishman, Sabrina Schreiner, Michelle Vincendeau

引用次数: 0

Endogenous retroelement expression in modeled airway epithelial repair. 内源性逆转录因子在模拟气道上皮修复中的表达。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-12-15 DOI: 10.1016/j.micinf.2024.105465

Stephanie Michael, Nicholas Liotta, Tongyi Fei, Matthew L Bendall, Douglas F Nixon, Nicholas Dopkins

{"title":"Endogenous retroelement expression in modeled airway epithelial repair.","authors":"Stephanie Michael, Nicholas Liotta, Tongyi Fei, Matthew L Bendall, Douglas F Nixon, Nicholas Dopkins","doi":"10.1016/j.micinf.2024.105465","DOIUrl":"10.1016/j.micinf.2024.105465","url":null,"abstract":"Cystic fibrosis (CF) is an autosomal recessive genetic disorder characterized by impairment of the CF transmembrane conductance regulator (CFTR) via gene mutation. CFTR is expressed at the cellular membrane of epithelial cells and functions as an anion pump which maintains water and salt ion homeostasis. In pulmonary airways of CF patients, pathogens such as P. aeruginosa and subsequent uncontrolled inflammation damage the human airway epithelial cells (HAECs) and can be life-threatening. We previously identified that inhibiting endogenous retroelement (ERE) reverse transcriptase can hamper the inflammatory response to bacterial flagella in THP-1 cells. Here, we investigate how ERE expression is sensitive to HAEC repair and toll-like receptor 5 (TLR5) activation, a primary mechanism by which inflammation impacts disease outcome. Our results demonstrate that several human endogenous retroviruses (HERVs) and long interspersed nuclear elements (LINEs) fluctuate throughout the various stages of repair and that TLR5 activation further influences ERE expression. By considering the impact of the most common CF mutation F508del/F508del on ERE expression in unwounded HAECs, we also found that two specific EREs, L1FLnI_2p23.1c and HERVH_10p12.33, were downregulated in CF-derived HAECs. Collectively, we show that ERE expression in HAECs is sensitive to certain modalities reflective of CF pathogenesis, and specific EREs may be indicative of CF disease state and pathogenesis.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105465"},"PeriodicalIF":2.6,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142837495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HERV-W envelope protein is present in microglial cells of the human glioma tumor microenvironment and differentially modulates neoplastic cell behavior. HERV-W 包膜蛋白存在于人类胶质瘤肿瘤微环境的小胶质细胞中，并对肿瘤细胞的行为起着不同的调节作用。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-20 DOI: 10.1016/j.micinf.2024.105460

Laura Reiche, Benedikt Plaack, Maike Lehmkuhl, Vivien Weyers, Joel Gruchot, Daniel Picard, Hervé Perron, Marc Remke, Christiane Knobbe-Thomsen, Guido Reifenberger, Patrick Küry, David Kremer

{"title":"HERV-W envelope protein is present in microglial cells of the human glioma tumor microenvironment and differentially modulates neoplastic cell behavior.","authors":"Laura Reiche, Benedikt Plaack, Maike Lehmkuhl, Vivien Weyers, Joel Gruchot, Daniel Picard, Hervé Perron, Marc Remke, Christiane Knobbe-Thomsen, Guido Reifenberger, Patrick Küry, David Kremer","doi":"10.1016/j.micinf.2024.105460","DOIUrl":"10.1016/j.micinf.2024.105460","url":null,"abstract":"Gliomas are the most common parenchymal tumors of the central nervous system (CNS). With regard to their still unclear etiology, several recent studies have provided evidence of a new category of pathogenic elements called human endogenous retroviruses (HERVs) which seem to contribute to the evolution and progression of many neurological diseases such as amyotrophic lateral sclerosis (ALS), schizophrenia, chronic inflammatory polyneuropathy (CIDP) and, particularly, multiple sclerosis (MS). In these diseases, HERVs exert effects on cellular processes such as inflammation, proliferation, and migration. In previous studies, we demonstrated that in MS, the human endogenous retrovirus type-W envelope protein (HERV-W ENV) interferes with lesion repair through the activation of microglia (MG), the innate myeloid immune cells of the CNS. Here, we now show that HERV-W ENV is also present in the microglial cells (MG) of the tumor microenvironment (TME) in gliomas. It modulates the behavior of glioblastoma (GBM) cell lines in GBM/MG cocultures by altering their gene expression, secreted cytokines, morphology, proliferation, and migration properties and could thereby contribute to key tumor properties.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105460"},"PeriodicalIF":2.6,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0