Microbes and Infection最新文献_第6页

Bacterial and host factors involved in zoonotic Streptococcal meningitis 人畜共患链球菌脑膜炎涉及的细菌和宿主因素

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2024.105335

Jiale Ma , Huizhen Wu , Zhe Ma , Zongfu Wu

{"title":"Bacterial and host factors involved in zoonotic Streptococcal meningitis","authors":"Jiale Ma , Huizhen Wu , Zhe Ma , Zongfu Wu","doi":"10.1016/j.micinf.2024.105335","DOIUrl":"10.1016/j.micinf.2024.105335","url":null,"abstract":"<div><div>Zoonotic streptococci cause several invasive diseases with high mortality rates, especially meningitis. Numerous studies elucidated the meningitis pathogenesis of zoonotic streptococci, some specific to certain bacterial species. In contrast, others are shared among different bacterial species, involving colonization and invasion of mucosal barriers, survival in the bloodstream, breaching the blood–brain and/or blood–cerebrospinal fluid barrier to access the central nervous system, and triggering inflammation of the meninges. This review focuses on the recent advancements in comprehending the molecular and cellular events of five major zoonotic streptococci responsible for causing meningitis in humans or animals, including <em>Streptococcus agalactiae</em>, <em>Streptococcus equi</em> subspecies <em>zooepidemicus</em>, <em>Streptococcus suis</em>, <em>Streptococcus dysgalactiae</em>, and <em>Streptococcus iniae</em>. The underlying mechanism was summarized into four themes, including 1) bacterial survival in blood, 2) brain microvascular endothelial cell adhesion and invasion, 3) penetration of the blood–brain barrier, and 4) activation of the immune system and inflammatory reaction within the brain. This review may contribute to developing therapeutics to prevent or mitigate injury of streptococcal meningitis and improve risk stratification.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105335"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140566419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copyright page Elsevier 版权页面Elsevier

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/S1286-4579(25)00006-1

引用次数: 0

Comparative genome analysis of Streptococcus suis serotype 5 strains from humans and pigs revealed pathogenic potential of virulent, antimicrobial resistance, and genetic relationship 来自人和猪的猪链球菌血清型 5 菌株的基因组比较分析揭示了毒力、抗菌药耐药性和遗传关系的致病潜力

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2023.105273

Anusak Kerdsin , Rujirat Hatrongjit , Thidathip Wongsurawat , Piroon Jenjaroenpun , Han Zheng , Peechanika Chopjitt , Parichart Boueroy , Nahuel Fittipaldi , Mariela Segura , Marcelo Gottschalk

{"title":"Comparative genome analysis of Streptococcus suis serotype 5 strains from humans and pigs revealed pathogenic potential of virulent, antimicrobial resistance, and genetic relationship","authors":"Anusak Kerdsin , Rujirat Hatrongjit , Thidathip Wongsurawat , Piroon Jenjaroenpun , Han Zheng , Peechanika Chopjitt , Parichart Boueroy , Nahuel Fittipaldi , Mariela Segura , Marcelo Gottschalk","doi":"10.1016/j.micinf.2023.105273","DOIUrl":"10.1016/j.micinf.2023.105273","url":null,"abstract":"<div><div><em>Streptococcus suis</em> is a causative agent of swine and human infections. Genomic analysis indicated that eight <em>S. suis</em> serotype 5 strains recovered from human patients and pigs carried many virulence-associated genes and markers defining pathogenic pathotypes. The strains were sequence types diverse and clustered within either minimum core genome group 3 (MCG-3) or MCG-7-3. Almost all the serotype 5 strains were non-susceptible to penicillin, ceftriaxone, erythromycin, and levofloxacin. Resistance to tetracycline and clindamycin was observed in all strains. The antimicrobial resistance genes <em>tet(O), tet(O/W/32/O), tet(W)</em>, <em>tet(44)</em>, <em>erm(B), ant(6)-Ia, lsaE</em>, and <em>lnuB</em> were found in these strains. Moderate-to-large numbers of substitutions were observed in three penicillin-binding proteins (PBP)—PBP1A, PBP2B, and PBP2X—in the penicillin-non-susceptible serotype 5 isolates that were involved in β-lactam-non-susceptibility. Comparative genomics between the serotype 5 and 2 strains revealed that only 15 genes absent from the serotype 2 strains were shared by all the serotype 5 strains. However, some additional genes were present only in some of the serotype 5 strains. This study highlighted the pathogenic potential of virulent serotype 5 strains in humans and pigs and the need for increased monitoring of penicillin-non-susceptibility in <em>S. suis</em> serotypes other than for serotype 2.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105273"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138558348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-species transmission and animal infection model of hepatitis E virus 戊型肝炎病毒的跨物种传播和动物感染模型。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2024.105338

Ling-Dong Xu , Fei Zhang , Pinglong Xu , Yao-Wei Huang

{"title":"Cross-species transmission and animal infection model of hepatitis E virus","authors":"Ling-Dong Xu , Fei Zhang , Pinglong Xu , Yao-Wei Huang","doi":"10.1016/j.micinf.2024.105338","DOIUrl":"10.1016/j.micinf.2024.105338","url":null,"abstract":"<div><div>Zoonotic hepatitis E virus (HEV) infection is an emerging global public health concern, and understanding the dynamics of HEV transmission between animals and humans is crucial for public health. Animal models are critical to advancing the understanding of HEV pathogenesis, drug screening, vaccine development, and other related areas. Here, we provide an overview of recent studies investigating the cross-species transmission of HEV, and also delve into the current research and application of animal HEV infection models including non-human primates, rodents, pigs, and chickens, offering a comprehensive assessment of the advantages and disadvantages of each model. This review highlights the findings related to viral replication, shedding patterns, and immune response in these animal models, and discusses the implications for our understanding of HEV transmission to humans. These advancements in the field enhance our understanding of the biological traits and pathogenic mechanisms of HEV, offering robust support for the development of highly effective and targeted prevention and treatment strategies.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105338"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140781412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Kinetic and proteomic studies in milk show distinct patterns among major Listeria monocytogenes clones 牛奶中的动力学和蛋白质组学研究显示，主要李斯特菌克隆之间存在不同的模式。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2024.105312

Alba Espí-Malillos , Carla Palacios-Gorba , Inmaculada López-Almela , Pilar Ruiz-García , María Carmen López-Mendoza , Francisco García-Del Portillo , M Graciela Pucciarelli , Juan J. Quereda

{"title":"Kinetic and proteomic studies in milk show distinct patterns among major Listeria monocytogenes clones","authors":"Alba Espí-Malillos , Carla Palacios-Gorba , Inmaculada López-Almela , Pilar Ruiz-García , María Carmen López-Mendoza , Francisco García-Del Portillo , M Graciela Pucciarelli , Juan J. Quereda","doi":"10.1016/j.micinf.2024.105312","DOIUrl":"10.1016/j.micinf.2024.105312","url":null,"abstract":"<div><div><em>Listeria monocytogenes</em>, a contaminant of raw milk, includes hypervirulent clonal complexes (CC) like CC1, CC4, and CC6, highly overrepresented in dairy products when compared to other food types. Whether their higher prevalence in dairy products is the consequence of a growth advantage in this food remains unknown. We examined growth kinetics of five <em>L. monocytogenes</em> isolates (CC1, CC4, CC6, CC9, and CC121) at 37 and 4 °C in ultra-high temperature (UHT) milk and raw milk. At 4 °C, hypovirulent CC9 and CC121 isolates exhibit better growth parameters in UHT milk compared to the hypervirulent CC1, CC4, and CC6 isolates. CC9 isolate in raw milk at 4 °C exhibited the fastest growth and the highest final concentrations. In contrast, hypervirulent isolates (CC1, CC4, and CC6) displayed better growth rates in UHT milk at 37 °C, the mammalian host temperature. Proteomic analysis of representative hyper- (CC1) and hypovirulent (CC9) isolates showed that they respond to milk cues differently with CC-specific traits. Proteins related to metabolism (such as LysA or different phosphotransferase systems), and stress response were upregulated in both isolates during growth in UHT milk. Our results show that there is a <em>Listeria</em> CC-specific and a <em>Listeria</em> CC-common response to the milk environment. These findings shed light on the overrepresentation of hypervirulent <em>L. monocytogenes</em> isolates in dairy products, suggesting that CC1 and CC4 overrepresentation in dairy products made of raw milk may arise from contamination during or after milking at the farm and discard an advantage of hypervirulent isolates in milk products when stored at refrigeration temperatures.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105312"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139723314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dual M protein systems have diverse biological characteristics, but both contribute to M18-type Group A Streptococcus pathogenicity 双M蛋白系统具有不同的生物学特性，但两者都有助于M18型A组链球菌的致病性。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2023.105209

Xiaorui Zhou , Haoshuai Song , Fei Pan , Chen Yuan , Lu Jia , Bing Wu , Hongjie Fan , Zhe Ma

{"title":"The dual M protein systems have diverse biological characteristics, but both contribute to M18-type Group A Streptococcus pathogenicity","authors":"Xiaorui Zhou , Haoshuai Song , Fei Pan , Chen Yuan , Lu Jia , Bing Wu , Hongjie Fan , Zhe Ma","doi":"10.1016/j.micinf.2023.105209","DOIUrl":"10.1016/j.micinf.2023.105209","url":null,"abstract":"<div><div>M protein is a key surface virulence factor in Group A Streptococcus (GAS), Group C Streptococcus (GCS), and other streptococcal species. GAS encodes M protein using the <em>emm</em> gene, while GCS employs the <em>szm</em> (or <em>sem</em>) gene. In M18-type GAS, dual M protein systems exist, comprising both GAS and GCS M proteins (encoded separately by <em>emm</em>18 and <em>spa</em>18). The <em>spa</em>18 gene in M18-type GAS shares a conserved region highly similar to GCS's <em>szm</em> gene. Our study reveals that <em>spa</em>18 exhibits higher transcription levels than <em>emm</em>18 in M18-type GAS strains. The dual M protein systems defective mutant (Δemm18Δspa18) displays a smooth surface, whereas wild-type and single M protein gene mutants remain rough. M18 and SPA18 proteins possess distinct characteristics, showing varied binding properties and cytotoxicity effects on macrophages (THP-1) and keratinocytes (HaCaT). Both <em>emm</em>18 and <em>spa</em>18 genes contribute to the skin pathogenicity of M18-type GAS. Transcriptome analysis suggests the potential involvement of the <em>mga</em> gene in <em>spa</em>18 transcription regulation, while <em>SpyM</em>18_2047 appears to be specific to <em>spa</em>18 regulation. In summary, this research offers a crucial understanding of the biological characteristics of dual M protein systems in M18-type GAS, highlighting their contributions to virulence and transcriptional regulation.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105209"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10030163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rab27a via its effector JFC1 localizes to Anaplasma inclusions and promotes Anaplasma proliferation in leukocytes Rab27a 通过其效应物 JFC1 定位于阿那普拉斯原虫包涵体并促进阿那普拉斯原虫在白细胞中的增殖

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2023.105278

Weiyan Huang, Mingqun Lin, Yasuko Rikihisa

{"title":"Rab27a via its effector JFC1 localizes to Anaplasma inclusions and promotes Anaplasma proliferation in leukocytes","authors":"Weiyan Huang, Mingqun Lin, Yasuko Rikihisa","doi":"10.1016/j.micinf.2023.105278","DOIUrl":"10.1016/j.micinf.2023.105278","url":null,"abstract":"<div><div><em>Anaplasma phagocytophilum</em> is an obligatory intracellular bacterium that causes tick-borne zoonosis called human granulocytic anaplasmosis. Mechanisms by which <em>Anaplasma</em> replicates inside of the membrane-bound compartment called “inclusion” in neutrophils are incompletely understood. A small GTPase Rab27a is found in the secretory granules and multivesicular endosomes. In this study we found Rab27a-containing granules were localized to <em>Anaplasma</em> inclusions in guanine nucleotide-dependent manner, and constitutively active Rab27a enhanced <em>Anaplasma</em> infection and dominant-negative Rab27a inhibited <em>Anaplasma</em> infection. Rab27a effector, JFC1 is known to mediate docking/fusion of Rab27a-bearing granules for exocytosis in leukocytes. shRNA stable knockdown of Rab27a or JFC1 inhibited <em>Anaplasma</em> infection in HL-60 cells. Similar to Rab27a, both endogenous and transfected JFC1 were localized to <em>Anaplasma</em> inclusions by immunostaining or live cell imaging. The JFC1 C2A domain that binds 3′-phosphoinositides, was sufficient and required for JFC1 and Rab27a localization to <em>Anaplasma</em> inclusions which were enriched with phosphatidylinositol 3-phosphate. Nexinhib20, the small molecule inhibitor specific to Rab27a and JFC1 binding, inhibited <em>Anaplasma</em> infection. Taken together, these results imply elevated phosphatidylinositol 3-phosphate in the inclusion membrane recruits JFC1 to mediate Rab27a-bearing granules/vesicles to dock/fuse with <em>Anaplasma</em> inclusions, the lumen of which is topologically equivalent to the exterior of the cell to benefit <em>Anaplasma</em> proliferation.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105278"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138682491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The functions of EF-hand proteins from host and zoonotic pathogens 宿主和人畜共患病原体的 EF-手蛋白的功能

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2023.105276

Wei Zuo , Mingxing Tian , Jingjing Qi , Guangdong Zhang , Jiangang Hu , Shaohui Wang , Yanqing Bao

引用次数: 0

Lineage classification and selective site identification of Orthoebolavirus zairense Orthoebolavirus zairense 的品系分类和选择性位点鉴定。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2024.105304

Jie Fang , Zhi-jian Zhou , Shuofeng Yuan , Ye Qiu , Xing-Yi Ge

{"title":"Lineage classification and selective site identification of Orthoebolavirus zairense","authors":"Jie Fang , Zhi-jian Zhou , Shuofeng Yuan , Ye Qiu , Xing-Yi Ge","doi":"10.1016/j.micinf.2024.105304","DOIUrl":"10.1016/j.micinf.2024.105304","url":null,"abstract":"<div><div>As the high pathogenic species of <em>Filoviridae</em> virus family, <em>Orthoebolavirus zairense</em> (EBOV) shows frequent outbreaks in human in recently years since its first emerging in 1976 in Democratic Republic of the Congo (COD), bringing ongoing risks and burden on public health safety. Here, the phylogenetic relationship among major outbreaks was analyzed. The results showed that EBOV isolates could be divided into four lineages according to spatial and temporal epidemics. Then, the positive selection sites (PSSs) were detected on all proteins of the EBOV, exhibiting lineage characteristic. Particularly, sites in GP and VP24 were identified to be significantly under positive selection, and partial of which were maintained in the latest isolates in 2021. GP and L were found to have high variability between lineages. Substitutions including F443L and F443S in GP, as well as F1610L and I1951V in L could be characteristic of the two large outbreaks in COD (2018) and West Africa (2014), respectively. Further, substitutions of significant PSSs in VP24 and L proteins were visualized for analysis of structural changes, which may affect EBOV pathogenesis. In summary, our results gains insights in genetic characteristic and adaptive evolution of EBOV, which could facilitate gene functional research against EBOV.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105304"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139567237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycerol metabolic repressor GlpR contributes to Streptococcus suis oxidative stress resistance and virulence 甘油代谢抑制因子 GlpR 对猪链球菌抗氧化压力和病毒性有贡献

IF 2.6 4区医学

Microbes and Infection Pub Date : 2025-01-01 DOI: 10.1016/j.micinf.2024.105307

Zijing Liang , Jiaxuan Lu , Yinli Bao , Xiang Chen , Huochun Yao , Zongfu Wu

{"title":"Glycerol metabolic repressor GlpR contributes to Streptococcus suis oxidative stress resistance and virulence","authors":"Zijing Liang , Jiaxuan Lu , Yinli Bao , Xiang Chen , Huochun Yao , Zongfu Wu","doi":"10.1016/j.micinf.2024.105307","DOIUrl":"10.1016/j.micinf.2024.105307","url":null,"abstract":"<div><div>Bacterial DeoR family transcription regulators regulate multiple physiological processes. Little is known about the function of DeoR family regulators in streptococci. Here, we identified a novel DeoR family regulator, GlpR, from <em>Streptococcus suis</em>, a pathogen causing severe diseases in pigs and humans. GlpR was involved in glycerol utilization and exhibited specific signature residues at positions 30–31 (KV) which are crucial for DNA binding. Deletion of <em>glpR</em> (Δ<em>glpR</em>) showed a significant increase in relative growth rate in glycerol medium compared to the wild-type (WT) and complementary strains (CΔ<em>glpR</em>). Employing RNA-seq analysis, β-galactosidase activity analysis, and electrophoretic mobility shift assay, we discovered that GlpR directly represses the expression of glycerol metabolism-related genes <em>pflB2</em>, <em>pflA1</em>, and <em>fsaA</em>, encoding pyruvate formate-lyase and its activating enzyme, and fructose-6-phosphate aldolase, respectively. Compared to WT and CΔ<em>glpR</em>, Δ<em>glpR</em> showed a reduced survival rate under oxidative stress and in murine macrophages and attenuated virulence in mice. GlpR probably enhances oxidative stress resistance and virulence in <em>S. suis</em> by functioning as a glycerol metabolic repressor decreasing energy consumption. These findings contribute to a better understanding of <em>S. suis</em> pathogenesis and enrich our knowledge of the biological functions of DeoR family regulators in streptococci.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"27 1","pages":"Article 105307"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139662958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0