Microbes and Infection最新文献_第7页

Factors underlying the long-term efficacy of faecal microbiota transplantation for patients with irritable bowel syndrome 粪便微生物群移植对肠易激综合征患者的长期疗效的基础因素。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105372

Magdy El-Salhy , Odd Helge Gilja , Jan Gunnar Hatlebakk

{"title":"Factors underlying the long-term efficacy of faecal microbiota transplantation for patients with irritable bowel syndrome","authors":"Magdy El-Salhy , Odd Helge Gilja , Jan Gunnar Hatlebakk","doi":"10.1016/j.micinf.2024.105372","DOIUrl":"10.1016/j.micinf.2024.105372","url":null,"abstract":"<div><div>The long-term effects of the transplant dose, its administration route and repeated faecal microbiota transplantation (FMT) on the outcomes of FMT for patients with irritable bowel syndrome (IBS) are unknown. This study included 171 patients (125 females and 46 males): 90 g of donor feces was administered into the large intestine (LI) in 58, into the small intestine (SI) in 57, and into the SI twice (repeated SI) in 56. The patients provided a fecal sample and completed five questionnaires at the baseline and at 2 years after FMT. Fecal bacteria and the dysbiosis index were analyzed using 16S rRNA gene PCR DNA amplification/probe. The response rates at 2 years after FMT were 47.2%, 80.9%, and 76.6% in the LI, SI, and repeated-SI groups, respectively. The response rate was significantly higher in the SI and repeated SI groups than in the LI group. IBS symptoms at 2 years after FMT were less severe in the SI and repeated-SI groups than in the LI group. Fluorescent signals of several bacteria were significantly correlated with IBS symptoms and fatigue after FMT. No long-term adverse events were observed. In conclusion, administering the transplant to the SI increased the long-term response rate and reduced IBS symptom severity compared with administering it to the LI, and led to the long-term colonization of beneficial bacteria. There was no long-term difference between one and two FMT procedures (www.clinicaltrials.gov<svg><path></path></svg>: NCT04236843).</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105372"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141284108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “N-acetyl-cysteine mediates protection against Mycobacterium avium through induction of human β-defensin-2” [Microb Infect 22 (10) (2020) 567–575] N-乙酰-半胱氨酸通过诱导人β-防御素-2介导对分枝杆菌的保护"[Microb Infect 22 (10) (2020) 567-575] 的更正。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105388

Ayako Shiozawa, Chiaki Kajiwara, Yoshikazu Ishii, Kazuhiro Tateda

引用次数: 0

Leishmania donovani modulates host miRNAs regulating cholesterol biosynthesis for its survival 唐氏利什曼原虫调节宿主的 miRNA，调控胆固醇的生物合成，以促进其生存

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105379

Shams Tabrez , Sajjadul Kadir Akand , Rahat Ali , Irshad Husain Naqvi , Neha Soleja , Mohd Mohsin , Mohammad Z. Ahmed , Mohammed Saleem , Suhel Parvez , Yusuf Akhter , Abdur Rub

{"title":"Leishmania donovani modulates host miRNAs regulating cholesterol biosynthesis for its survival","authors":"Shams Tabrez , Sajjadul Kadir Akand , Rahat Ali , Irshad Husain Naqvi , Neha Soleja , Mohd Mohsin , Mohammad Z. Ahmed , Mohammed Saleem , Suhel Parvez , Yusuf Akhter , Abdur Rub","doi":"10.1016/j.micinf.2024.105379","DOIUrl":"10.1016/j.micinf.2024.105379","url":null,"abstract":"<div><div>Cholesterol reduction by intracellular protozoan parasite Leishmania donovani (L. donovani), causative agent of leishmaniasis, impairs antigen presentation, pro-inflammatory cytokine secretion and host-protective membrane-receptor signaling in macrophages. Here, we studied the miRNA mediated regulation of cholesterol biosynthetic genes to understand the possible mechanism of L. donovani-induced cholesterol reduction and therapeutic importance of miRNAs in leishmaniasis. System-scale genome-wide microtranscriptome screening was performed to identify the miRNAs involved in the regulation of expression of key cholesterol biosynthesis regulatory genes through miRanda3.0. 11 miRNAs out of 2823, showing complementarity with cholesterol biosynthetic genes were finally selected for expression analysis. These selected miRNAs were differentially regulated in THP-1 derived macrophages and in primary human macrophages by L. donovani. Correlation of expression and target validation through luciferase assay suggested two key miRNAs, hsa-miR-1303 and hsa-miR-874-3p regulating the key genes hmgcr and hmgcs1 respectively. Inhibition of hsa-mir-1303 and hsa-miR-874-3p augmented the expression of targets and reduced the parasitemia in macrophages. This study will also provide the platform for the development of miRNA-based therapy against leishmaniasis.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105379"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141398578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intranasal immunization with poly I:C and CpG ODN adjuvants enhances the protective efficacy against Helicobacter pylori infection in mice. 使用聚 I:C 和 CpG ODN 佐剂进行鼻内免疫可增强小鼠对幽门螺旋杆菌感染的保护效力。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-10-24 DOI: 10.1016/j.micinf.2024.105433

Min Sun, Yu Liu, Xiumei Ni, Runqing Tan, Yi Wang, Yajun Jiang, Dingxin Ke, Han Du, Gang Guo, Kaiyun Liu

{"title":"Intranasal immunization with poly I:C and CpG ODN adjuvants enhances the protective efficacy against Helicobacter pylori infection in mice.","authors":"Min Sun, Yu Liu, Xiumei Ni, Runqing Tan, Yi Wang, Yajun Jiang, Dingxin Ke, Han Du, Gang Guo, Kaiyun Liu","doi":"10.1016/j.micinf.2024.105433","DOIUrl":"10.1016/j.micinf.2024.105433","url":null,"abstract":"Helicobacter pylori (H. pylori) infection is a serious public health issue, and development of vaccines is a desirable preventive strategy for H. pylori. Toll-like receptor (TLR) ligands have shown potential as vaccine adjuvants that induce immune responses, but polyinosinic-polycytidylic acid (poly I:C), a nucleic acid-based TLR9 ligand, is less well studied in H. pylori vaccine research. Here, we evaluated the effects of poly I:C and CpG oligodeoxynucleotide (CpG ODN), a nucleic acid TLR3 ligand, as adjuvants in combination with the H. pylori recombinant proteins LpoB and UreA to protect against H. pylori infection. For analysis of specific immune responses, the levels of specific antibodies and splenic cytokines were measured in the immunized mice. Compared with CpG ODN, poly I:C could induce mucosal sIgA antibody responses and reduce H. pylori colonization. Additionally, the combination of poly I:C and CpG ODN caused greater immunoprotection and significantly reduced gastritis, exerting synergistic effects. Analysis of splenic cytokines revealed that poly I:C mainly triggered a mixed Th1/Th2/Th17 immune response, whereas the combination of CpG ODN and poly I:C induced a Th1/Th17 immune response. Our findings indicated that increased levels of mucosal sIgA antibodies and a robust splenic Th1/Th17 immune response were associated with reduced H. pylori colonization in vaccinated mice. This study identified a potential TLR ligand adjuvant for developing more effective H. pylori vaccines.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105433"},"PeriodicalIF":2.6,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142503630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The impact of anti-tuberculosis treatment on respiratory tract microbiome in pulmonary tuberculosis. 抗结核治疗对肺结核呼吸道微生物组的影响。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-10-19 DOI: 10.1016/j.micinf.2024.105432

Druti Hazra, Kiran Chawla, Fayaz S M, Vitali Sintchenko, Rahul Magazine, Elena Martinez, Akhilesh Pandey

{"title":"The impact of anti-tuberculosis treatment on respiratory tract microbiome in pulmonary tuberculosis.","authors":"Druti Hazra, Kiran Chawla, Fayaz S M, Vitali Sintchenko, Rahul Magazine, Elena Martinez, Akhilesh Pandey","doi":"10.1016/j.micinf.2024.105432","DOIUrl":"10.1016/j.micinf.2024.105432","url":null,"abstract":"The growing evidence has underscored the significance of interactions between the host and microbiota in respiratory health, presenting a novel perspective on disease management. Yet, comprehension of the respiratory microbiome shifts before and after anti-tuberculosis treatment is limited. This study compares respiratory microbiome profiles in untreated tuberculosis (UTB) and completed TB treatment (CTB) cases with healthy controls, using 16S rRNA sequencing on sputum samples. Significant reduction in sputum microbial alpha diversity was observed in both TB groups when compared to healthy controls (P < 0.05). Beta diversity analysis showed distinct clustering (P < 0.05). Linear discriminant analysis revealed an abundance of potentially pathogenic bacterial genera like Haemophilus, Pseudomonas, and Mycobacterium in the UTB group, while Streptococcus, Rothia, and Neisseria dominated in CTB samples. Healthy sputum microbiomes were enriched with Prevotella, Fusobacterium, Porphyromonadaceae_unclassified,andPeptostreptococcus. Moreover, predicted bacterial functional pathways showed significant differences among the three groups, mainly related to nutrient metabolism. These findings indicated significant microbial dysbiosis in sputum samples recovered from patients with pulmonary TB with an elevated presence of potentially pathogenic bacteria, depletion of beneficial genera, and downregulation of several essential metabolic pathways. Further exploration of respiratory microbiome-based diagnostic biomarkers and their role in targeted treatment strategies in tuberculosis is warranted.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105432"},"PeriodicalIF":2.6,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

HERV-W ENV transcription in B cells predicting symptomatic COVID-19 and risk for long COVID can express a full-length protein despite stop codon in mRNA from chromosome X via a ribosome readthrough. HERV-W ENV 在 B 细胞中的转录可预测有症状的 COVID-19 和长 COVID 风险，尽管来自 X 染色体的 mRNA 中存在终止密码子，但 HERV-W ENV 仍可通过核糖体通读表达全长蛋白质。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-10-16 DOI: 10.1016/j.micinf.2024.105431

Joanna Brunel, Julien Paganini, Melissa Galloux, Benjamin Charvet, Hervé Perron

{"title":"HERV-W ENV transcription in B cells predicting symptomatic COVID-19 and risk for long COVID can express a full-length protein despite stop codon in mRNA from chromosome X via a ribosome readthrough.","authors":"Joanna Brunel, Julien Paganini, Melissa Galloux, Benjamin Charvet, Hervé Perron","doi":"10.1016/j.micinf.2024.105431","DOIUrl":"10.1016/j.micinf.2024.105431","url":null,"abstract":"The human genome comprises 8 % of endogenous retroviruses (HERVs). Though HERVS contribute to physiological functions, copies retained pathogenic potential. The HERV-W ENV protein was shown expressed in patients with worse COVID-19 symptoms and post-COVID syndrome. A significant detection of the mRNA encoding HERV-W ENV from patients with COVID-19 in B cells from RNAseq reads obtained from peripheral blond mononuclear cells. This data stratified with increased COVID-19 symptoms or with post-acute sequelae of COVID-19 (long COVID) after 3 months. The HERV-W ENV-U3R RNA was confirmed to display the best alignment with chromosome X ERVWE2 locus. However, a stop codon precluding its translation was re-addressed after recent understandings of ribosome readthrough mechanisms. Experimental results evidenced that this HERV gene can effectively express a full-length protein in the presence of molecules allowing translation via a readthrough mechanism at the ribosome level. Results not only confirm HERV-W ENV RNA origin in these patients but show for the first time how a defective HERV copy can be translated into a complete protein when specific factors make it possible at the ribosome level. The present proof of concept now requires further studies to identify the factors involved in this newly understood mechanism, following SARS-CoV-2 exposure.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105431"},"PeriodicalIF":2.6,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142469725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppressive effects of toll-like receptor 2, toll-like receptor 4, and toll-like receptor 7 on protective responses to Mycobacterium bovis BCG from epithelial cells. Toll-Like Receptor 2、Toll-Like Receptor 4 和 Toll-Like Receptor 7 对上皮细胞卡介苗分枝杆菌保护性反应的抑制作用。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-10-04 DOI: 10.1016/j.micinf.2024.105428

Aarti Singh, Akshita Singh, Shakuntala Surender Kumar Saraswati, Ankush Kumar Rana, Aayushi Singh, Chaitenya Verma, Vishal Sinha, Kanika Kalra, Krishnamurthy Natarajan

{"title":"Suppressive effects of toll-like receptor 2, toll-like receptor 4, and toll-like receptor 7 on protective responses to Mycobacterium bovis BCG from epithelial cells.","authors":"Aarti Singh, Akshita Singh, Shakuntala Surender Kumar Saraswati, Ankush Kumar Rana, Aayushi Singh, Chaitenya Verma, Vishal Sinha, Kanika Kalra, Krishnamurthy Natarajan","doi":"10.1016/j.micinf.2024.105428","DOIUrl":"10.1016/j.micinf.2024.105428","url":null,"abstract":"Mycobacteria have several mechanisms for evasion of protective responses mounted by the host. In this study, we unravel yet another mechanism that is mediated by Toll-Like Receptors TLR2, TLR4, and TLR7 in epithelial cells. We show that mycobacterial infection of epithelial cells increases the expression of TLR2, TLR4, and TLR7. Stimulation of either TLR along with mycobacterial infection results in an inhibition of oxidative burst resulting in increased survival of mycobacteria inside epithelial cells. TLR stimulation along with mycobacterial infection also inhibits activation of epithelial cells for T cell responses by differentially regulating the activation of ERK-MAPK and p38-MAPK along with inhibition of co-stimulatory molecule CD86 expression. Furthermore, stimulation of either TLR inhibits the induction of apoptosis and autophagy. Knockdown of either TLR by specific siRNAs reverses the inhibition by ROS and apoptosis by mycobacteria and results in reduced intracellular survival of mycobacteria in a MyD88-dependent manner. These results point towards a negative role for TLR2, TLR4, and TLR7 in regulating protective responses to M. bovis BCG infection in epithelial cells.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105428"},"PeriodicalIF":2.6,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dopaminergic neuronal regulation determines innate immunity of Caenorhabditis elegans during Klebsiella aerogenes infection. 多巴胺能神经元调控决定了草履虫在产气克雷伯氏菌感染期间的先天免疫力。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-10-04 DOI: 10.1016/j.micinf.2024.105430

Thirumugam Gowripriya, Radhakrishnan Yashwanth, Prabhanand Bhaskar James, Ramamurthi Suresh, Krishnaswamy Balamurugan

{"title":"Dopaminergic neuronal regulation determines innate immunity of Caenorhabditis elegans during Klebsiella aerogenes infection.","authors":"Thirumugam Gowripriya, Radhakrishnan Yashwanth, Prabhanand Bhaskar James, Ramamurthi Suresh, Krishnaswamy Balamurugan","doi":"10.1016/j.micinf.2024.105430","DOIUrl":"10.1016/j.micinf.2024.105430","url":null,"abstract":"The innate immune signals are the front line of host defense against bacterial pathogens. Pathogen-induced harmful effects, such as reduced neuronal signals to the intestine, affect the host's food sensing and dwelling behavior. Here, we report that dopamine and kpc-1 signals control the intestinal innate immune responses through the p38/PMK-1 MAPK signaling pathway in C. elegans. K. aerogenes infection in C. elegans affects the food-dwelling behavior, which depends on dopamine regulation. The absence of the dopamine receptor (dop-1) and transporter (dat-1) increases attraction to the pathogen instead of avoidance. The K. aerogenes infection affects age-1 regulation through the furin-like proprotein convertase (kpc-1); the absence of kpc-1 affects environment-dependent dauer formation. In contrast, the dop-1 mutation antagonistically regulates intestinal immune regulation, while the kpc-1 mutation partially regulates the p38/PMK-1 MAPK pathway. Our findings indicate that dopamine and kpc-1signaling from the nervous system control intestinal immunity in an antagonistic and agonistic manner, respectively.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105430"},"PeriodicalIF":2.6,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and in silico characterization of prophages in Helicobacter pylori clinical strains. 幽门螺旋杆菌临床菌株中噬菌体的筛选和硅学表征。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-10-03 DOI: 10.1016/j.micinf.2024.105429

Rute Ferreira, Graça Pinto, Eva Presa, Mónica Oleastro, Catarina Silva, Luís Vieira, Cláudia Sousa, Diana P Pires, Ceu Figueiredo, Luís D R Melo

{"title":"Screening and in silico characterization of prophages in Helicobacter pylori clinical strains.","authors":"Rute Ferreira, Graça Pinto, Eva Presa, Mónica Oleastro, Catarina Silva, Luís Vieira, Cláudia Sousa, Diana P Pires, Ceu Figueiredo, Luís D R Melo","doi":"10.1016/j.micinf.2024.105429","DOIUrl":"10.1016/j.micinf.2024.105429","url":null,"abstract":"The increase of antibiotic resistance calls for alternatives to control Helicobacter pylori, a Gram-negative bacterium associated with various gastric diseases. Bacteriophages (phages) can be highly effective in the treatment of pathogenic bacteria. Here, we developed a method to identify prophages in H. pylori genomes aiming at their future use in therapy. A polymerase chain reaction (PCR)-based technique tested five primer pairs on 74 clinical H. pylori strains. After the PCR screening, 14 strains most likely to carry prophages were fully sequenced. After that, a more holistic approach was taken by studying the complete genome of the strains. This study allowed us to identify 12 intact prophage sequences, which were then characterized concerning their morphology, virulence, and antibiotic-resistance genes. To understand the variability of prophages, a phylogenetic analysis using the sequences of all H. pylori phages reported to date was performed. Overall, we increased the efficiency of identifying complete prophages to 54.1 %. Genes with homology to potential virulence factors were identified in some new prophages. Phylogenetic analysis revealed a close relationship among H. pylori-phages, although there are phages with different geographical origins. This study provides a deeper understanding of H. pylori-phages, providing valuable insights into their potential use in therapy.","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":" ","pages":"105429"},"PeriodicalIF":2.6,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142378063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk between human endogenous retroviruses and exogenous viruses. 人类内源性逆转录病毒与外源性病毒之间的相互影响。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-09-28 DOI: 10.1016/j.micinf.2024.105427

Edoardo Pizzioli, Antonella Minutolo, Emanuela Balestrieri, Claudia Matteucci, Gkikas Magiorkinis, Branka Horvat

引用次数: 0