Microbes and Infection最新文献_第7页

Unraveling the role of human microglia in tick-borne encephalitis virus infection: insights into neuroinflammation and viral pathogenesis 揭示人类小胶质细胞在蜱传脑炎病毒感染中的作用：洞察神经炎症和病毒发病机理。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105383

Veronika Pranclova , Lenka Nedvedova , Eliska Kotounova , Vaclav Hönig , Marketa Dvorakova , Marika Davidkova , Tomas Bily , Marie Vancova , Daniel Ruzek , Martin Palus

{"title":"Unraveling the role of human microglia in tick-borne encephalitis virus infection: insights into neuroinflammation and viral pathogenesis","authors":"Veronika Pranclova , Lenka Nedvedova , Eliska Kotounova , Vaclav Hönig , Marketa Dvorakova , Marika Davidkova , Tomas Bily , Marie Vancova , Daniel Ruzek , Martin Palus","doi":"10.1016/j.micinf.2024.105383","DOIUrl":"10.1016/j.micinf.2024.105383","url":null,"abstract":"<div><div>Tick-borne encephalitis virus (TBEV) is a neurotropic orthoflavivirus responsible for severe infections of the central nervous system. Although neurons are predominantly targeted, specific involvement of microglia in pathogenesis of TBE is not yet fully understood. In this study, the susceptibility of human microglia to TBEV is investigated, focusing on productive infection and different immune responses of different viral strains. We investigated primary human microglia and two immortalized microglial cell lines exposed to three TBEV strains (Hypr, Neudörfl and 280), each differing in virulence. Our results show that all microglia cultures tested support long-term productive infections, regardless of the viral strain. In particular, immune response varied significantly with the viral strain, as shown by the differential secretion of cytokines and chemokines such as IP-10, MCP-1, IL-8 and IL-6, quantified using a Luminex 48-plex assay. The most virulent strain triggered the highest cytokine induction. Electron tomography revealed substantial ultrastructural changes in the infected microglia, despite the absence of cytopathic effects. These findings underscore the susceptibility of human microglia to TBEV and reveal strain-dependent variations in viral replication and immune responses, highlighting the complex role of microglia in TBEV-induced neuropathology and contribute to a deeper understanding of TBE pathogenesis and neuroinflammation.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105383"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141469342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trypanosoma cruzi Vps34 colocalizes with Beclin1 and plays a role in parasite invasion of the host cell by modulating the expression of a sub-group of trans-sialidases 克氏锥虫 Vps34 与 Beclin1 共定位，通过调节反式苷酸酶亚群的表达，在寄生虫入侵宿主细胞的过程中发挥作用。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105385

Carlos Alcides Nájera , Mercedes Soares-Silva , Fernando Y. Maeda , Wanderson Duarte DaRocha , Isabela Meneghelli , Ana Clara Mendes , Marina Ferreira Batista , Claudio Vieira Silva , José Franco da Silveira , Cristina M. Orikaza , Nobuko Yoshida , Viviane Grazielle Silva , Santuza Maria Ribeiro Teixeira , Daniella Castanheira Bartholomeu , Diana Bahia

{"title":"Trypanosoma cruzi Vps34 colocalizes with Beclin1 and plays a role in parasite invasion of the host cell by modulating the expression of a sub-group of trans-sialidases","authors":"Carlos Alcides Nájera , Mercedes Soares-Silva , Fernando Y. Maeda , Wanderson Duarte DaRocha , Isabela Meneghelli , Ana Clara Mendes , Marina Ferreira Batista , Claudio Vieira Silva , José Franco da Silveira , Cristina M. Orikaza , Nobuko Yoshida , Viviane Grazielle Silva , Santuza Maria Ribeiro Teixeira , Daniella Castanheira Bartholomeu , Diana Bahia","doi":"10.1016/j.micinf.2024.105385","DOIUrl":"10.1016/j.micinf.2024.105385","url":null,"abstract":"<div><div><em>Trypanosoma cruzi</em>, the etiological agent of Chagas' disease, can infect both phagocytic and non-phagocytic cells. <em>T. cruzi</em> gp82 and gp90 are cell surface proteins belonging to Group II <em>trans</em>-sialidases known to be involved in host cell binding and invasion. Phosphatidylinositol kinases (PIK) are lipid kinases that phosphorylate phospholipids in their substrates or in themselves, regulating important cellular functions such as metabolism, cell cycle and survival. Vps34, a class III PIK, regulates autophagy, trimeric G-protein signaling, and the mTOR (mammalian Target of Rapamycin) nutrient-sensing pathway. The mammalian autophagy gene Beclin1 interacts to Vps34 forming Beclin 1–Vps34 complexes involved in autophagy and protein sorting. In <em>T. cruzi</em> epimastigotes, (a <em>non</em>-infective replicative form), TcVps34 has been related to morphological and functional changes associated to vesicular trafficking, osmoregulation and receptor-mediated endocytosis. We aimed to characterize the role of TcVps34 during invasion of HeLa cells by metacyclic (MT) forms. MTs overexpressing TcVps34 showed lower invasion rates compared to controls, whilst exhibiting a significant decrease in gp82 expression in the parasite surface. In addition, we showed that <em>T. cruzi</em> Beclin (TcBeclin1) colocalizes with TcVps34 in epimastigotes, thus suggesting the formation of complexes that may play conserved cellular roles already described for other eukaryotes.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105385"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicles from primary human macrophages stimulated with VIP or PACAP mediate anti-SARS-CoV-2 activities in monocytes through NF-κB signaling pathway 受到 VIP 或 PACAP 刺激的原代人类巨噬细胞的细胞外囊泡通过 NF-κB 信号通路介导单核细胞的抗 SARS-CoV-2 活性。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105400

Luis A. Arteaga-Blanco , Jairo R. Temerozo , Lucas P.S. Tiné , Luíza Dantas-Pereira , Carolina Q. Sacramento , Natalia Fintelman-Rodrigues , Beatriz M. Toja , Suelen Silva Gomes Dias , Caroline S. de Freitas , Camila Couto Espírito-Santo , Ygor P. Silva , Rudimar L. Frozza , Patrícia T. Bozza , Rubem F.S. Menna-Barreto , Thiago Moreno L. Souza , Dumith Chequer Bou-Habib

{"title":"Extracellular vesicles from primary human macrophages stimulated with VIP or PACAP mediate anti-SARS-CoV-2 activities in monocytes through NF-κB signaling pathway","authors":"Luis A. Arteaga-Blanco , Jairo R. Temerozo , Lucas P.S. Tiné , Luíza Dantas-Pereira , Carolina Q. Sacramento , Natalia Fintelman-Rodrigues , Beatriz M. Toja , Suelen Silva Gomes Dias , Caroline S. de Freitas , Camila Couto Espírito-Santo , Ygor P. Silva , Rudimar L. Frozza , Patrícia T. Bozza , Rubem F.S. Menna-Barreto , Thiago Moreno L. Souza , Dumith Chequer Bou-Habib","doi":"10.1016/j.micinf.2024.105400","DOIUrl":"10.1016/j.micinf.2024.105400","url":null,"abstract":"<div><div>Infection by SARS-CoV-2 is associated with uncontrolled inflammatory response during COVID-19 severe disease, in which monocytes are one of the main sources of pro-inflammatory mediators leading to acute respiratory distress syndrome. Extracellular vesicles (EVs) from different cells play important roles during SARS-CoV-2 infection, but investigations describing the involvement of EVs from primary human monocyte-derived macrophages (MDM) on the regulation of this infection are not available. Here, we describe the effects of EVs released by MDM stimulated with the neuropeptides VIP and PACAP on SARS-CoV-2-infected monocytes. MDM-derived EVs were isolated by differential centrifugation of medium collected from cells cultured for 24 h in serum-reduced conditions. Based on morphological properties, we distinguished two subpopulations of MDM-EVs, namely large (LEV) and small EVs (SEV). We found that MDM-derived EVs stimulated with the neuropeptides inhibited SARS-CoV-2 RNA synthesis/replication in monocytes, protected these cells from virus-induced cytopathic effects and reduced the production of pro-inflammatory mediators. In addition, EVs derived from VIP- and PACAP-treated MDM prevented the SARS-CoV-2-induced NF-κB activation. Overall, our findings suggest that MDM-EVs are endowed with immunoregulatory properties that might contribute to the antiviral and anti-inflammatory responses in SARS-CoV-2-infected monocytes and expand our knowledge of EV effects during COVID-19 pathogenesis.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105400"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of novel HIV fusion-inhibitory lipopeptides with the M-T hook structure 具有 M-T 钩结构的新型 HIV 融合抑制脂肽的特征。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105366

Xiuzhu Geng , Xiaohui Ding , Yuanmei Zhu , Huihui Chong , Yuxian He

{"title":"Characterization of novel HIV fusion-inhibitory lipopeptides with the M-T hook structure","authors":"Xiuzhu Geng , Xiaohui Ding , Yuanmei Zhu , Huihui Chong , Yuxian He","doi":"10.1016/j.micinf.2024.105366","DOIUrl":"10.1016/j.micinf.2024.105366","url":null,"abstract":"<div><div><span><span>Combination antiretroviral therapy (cART) has significantly improved the survival of HIV-infected individuals, but long-term treatment can cause side-effects and drug resistance; thus, the development of new antivirals is of importance. We previously identified an M-T hook structure and accordingly designed short-peptide fusion inhibitor<span><span> 2P23, which mainly targets the gp41<span> pocket site and displays potent, broad-spectrum anti-HIV activity. In this study, we continuingly characterized the amino acid sequences of peptide and lipopeptide-based inhibitors containing the M-T hook residues. Among a group of lipopeptides, </span></span>stearic acid (C18)-modified LP-25 and LP-29 exhibited greatly improved inhibitions against divergent HIV-1 subtypes and drug-resistant mutants. LP-25 and LP-29 were evaluated in </span></span>rhesus macaques, and the </span><em>ex vivo</em> inhibition data demonstrated their potent, long-lasting <em>in vivo</em><span> anti-HIV activity, with LP-25 much better than LP-29. Both the lipopeptides displayed high α-helicity, thermostability<span> and binding ability to a target-mimic peptide, and they were metabolically stable when treated with high temperature, proteolytic enzymes<span><span>, human or monkey sera and human liver microsomes. Therefore, our studies have provided critical information for understanding the structure-activity relationship of HIV fusion inhibitors with the M-T hook structure and offered novel candidates for </span>drug development.</span></span></span></div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105366"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-190 restores the innate immune homeostasis of Drosophila by directly inhibiting Tab2 in Imd pathway miR-190 通过直接抑制 Imd 通路中的 Tab2 恢复果蝇的先天性免疫平衡。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105399

Xiaolong Yao , Yuqing He , Canhe Zhu , Shangmin Yang , Jing Wu , Fei Ma , Ping Jin

{"title":"miR-190 restores the innate immune homeostasis of Drosophila by directly inhibiting Tab2 in Imd pathway","authors":"Xiaolong Yao , Yuqing He , Canhe Zhu , Shangmin Yang , Jing Wu , Fei Ma , Ping Jin","doi":"10.1016/j.micinf.2024.105399","DOIUrl":"10.1016/j.micinf.2024.105399","url":null,"abstract":"<div><div>The <em>Drosophila</em> Imd pathways are well-known mechanisms involved in innate immunity responsible for Gram-negative (G-) bacterial infection. The intensity and durability of immunity need to be finely regulated to keep sufficient immune activation meanwhile avoid excessive immune response. In this study, we firstly demonstrated that miR-190 can downregulate the expression levels of antimicrobial peptides (AMPs) in the Imd immune pathway after <em>Escherichia coli</em> infection using the miR-190 overexpression flies and the miR-190KO/+ flies. Secondly, miR-190 overexpression significantly reduces while miR-190 KO increases <em>Drosophila</em> survival rates upon lethal <em>Enterobacter cloacae</em> infection. Thirdly, we further demonstrated that miR-190 negatively regulates innate immune responses by directly targeting both RA/RB and RC isoforms of <em>Tab2.</em> In addition, the dynamic expression pattern of AMPs (<em>Dpt</em>, <em>AttA</em>, <em>CecA1</em>), <em>miR-190</em> and <em>Tab2</em> in the wild-type flies reveals that miR-190 play an important role in <em>Drosophila</em> immune homeostasis restoration at the late stage of <em>E. coli</em> infection. Collectively, our study reveals that miR-190 can downregulate the expression of AMPs by targeting <em>Tab2</em> and promote immune homeostasis restoration in <em>Drosophila</em> Imd pathway. Our study provides new insights into the regulatory mechanism of animal innate immune homeostasis.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105399"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141860204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conference report: the first bacterial genome sequencing pan-European network conference 会议报告：第一届泛欧细菌基因组测序网络会议。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105410

Zoja Germuskova , Elisa Sosa , Amaya Campillay Lagos , Hege Vangstein Aamot , Mathew A. Beale , Claire Bertelli , Jonas Björkmann , Natacha Couto , Lena Feige , Gilbert Greub , Erika Tång Hallbäck , Emma B. Hodcroft , Dag Harmsen , Laurent Jacob , Keith A. Jolley , Andre Kahles , Alison E. Mather , Richard A. Neher , Aitana Neves , Stefan Niemann , Adrian Egli

引用次数: 0

Copyright page Elsevier 版权页面Elsevier

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/S1286-4579(24)00186-2

引用次数: 0

Characterization and dynamics of intestinal microbiota in patients with Clostridioides difficile colonization and infection 艰难梭菌定植和感染患者肠道微生物群的特征和动态变化。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105373

Yanzi Zhou , Lihua Guo , Tingting Xiao , Yunbo Chen , Tao Lv , Yuan Wang , Shuntian Zhang , Hongliu Cai , Xiaohui Chi , Xiaoyang Kong , Kai Zhou , Ping Shen , Yonghong Xiao

{"title":"Characterization and dynamics of intestinal microbiota in patients with Clostridioides difficile colonization and infection","authors":"Yanzi Zhou , Lihua Guo , Tingting Xiao , Yunbo Chen , Tao Lv , Yuan Wang , Shuntian Zhang , Hongliu Cai , Xiaohui Chi , Xiaoyang Kong , Kai Zhou , Ping Shen , Yonghong Xiao","doi":"10.1016/j.micinf.2024.105373","DOIUrl":"10.1016/j.micinf.2024.105373","url":null,"abstract":"<div><div>Gut microbiota dysbiosis increases the susceptibility to <em>Clostridioides difficile</em> infection (CDI). In this study, we monitored <em>C. difficile</em> colonization (CDC) patients from no CDC status (CDN) to CDC status (CDCp) and CDI patients from asymptomatic status before CDI (PRECDI), CDI status (ONCDI), to asymptomatic status after CDI (POSTCDI). Based on metagenomic sequencing, we aimed to investigate the interaction pattern between gut microbiota and <em>C. difficile</em>. There was no significant difference of microbiota diversity between CDN and CDCp. In CDCp, Bacteroidetes and short-chain fatty acid (SCFA)-producing bacteria increased, with a positive correlation between SCFA-producing bacteria and <em>C. difficile</em> colonization. Compared with PRECDI, ONCDI and POSTCDI showed a significant decrease in microbiota diversity, particularly in Bacteroidetes and SCFA-producing bacteria, with a positive correlation between opportunistic pathogen and <em>C. difficile</em>. Fatty acid metabolism, and amino acid biosynthesis were enriched in CDN, CDCp, and PRECDI, while bile secretion was enriched in ONCDI and POSTCDI. Microbiota and metabolic pathways interaction networks in CDN and CDCp were more complex, particularly pathways in fatty acid and bile acid metabolism. Increasing of Bacteroidetes and SCFA-producing bacteria, affecting amino acid and fatty acid metabolism, is associated with colonization resistance to <em>C. difficile</em> and inhibiting the development of CDI.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105373"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141301057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of microRNAs in immune regulation and pathogenesis of Chlamydia trachomatis and Chlamydia muridarum infections: a rapid review 微RNA在沙眼衣原体和鼠衣原体感染的免疫调节和发病机制中的作用：快速综述。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105397

Chloe Meewes, Kanupriya Gupta, William M. Geisler

引用次数: 0

Integrated analysis of microbiome and host transcriptome unveils correlations between lung microbiota and host immunity in bronchoalveolar lavage fluid of pneumocystis pneumonia patients 对微生物组和宿主转录组的综合分析揭示了肺孢子菌肺炎患者支气管肺泡灌洗液中肺微生物组与宿主免疫之间的相关性。

IF 2.6 4区医学

Microbes and Infection Pub Date : 2024-11-01 DOI: 10.1016/j.micinf.2024.105374

Ling Zhang , Miaotian Cai , Xin Zhang , Sitong Wang , Lijun Pang , Xue Chen , Caopei Zheng , Yuqing Sun , Ying Liang , Shan Guo , Feili Wei , Yulin Zhang

{"title":"Integrated analysis of microbiome and host transcriptome unveils correlations between lung microbiota and host immunity in bronchoalveolar lavage fluid of pneumocystis pneumonia patients","authors":"Ling Zhang , Miaotian Cai , Xin Zhang , Sitong Wang , Lijun Pang , Xue Chen , Caopei Zheng , Yuqing Sun , Ying Liang , Shan Guo , Feili Wei , Yulin Zhang","doi":"10.1016/j.micinf.2024.105374","DOIUrl":"10.1016/j.micinf.2024.105374","url":null,"abstract":"<div><h3>Objective</h3><div><span><span>The lung microbiota of patients with </span>pulmonary diseases<span> is disrupted and impacts the immunity. The microbiological and immune landscape of the lungs in patients with </span></span>pneumocystis pneumonia (PCP) remains poorly understood.</div></div><div><h3>Methods</h3><div><span>Multi-omics analysis and machine learning were performed on bronchoalveolar lavage fluid<span> to explore interaction between the lung microbiota and host immunity in PCP. Then we constructed a diagnostic model using differential genes with LASSO regression and validated by </span></span>qPCR<span>. The immune infiltration analysis was performed to explore the landscape of lung immunity in patients with PCP.</span></div></div><div><h3>Results</h3><div><span>Patients with PCP showed a low alpha diversity of lung microbiota, accompanied by the elevated abundance of </span><span><span>Firmicutes</span></span><span><span>, and the differential expressed genes (DEGs) analysis displayed a downregulation of </span>MAPK signaling<span><span>. The MAPK10, TGFB1, and </span>EFNA3 indicated a potential to predict PCP (AUC = 0.86). The lung immune landscape in PCP showed the lower levels of naïve CD4</span></span><sup>+</sup><span> T cells<span> and activated dendritic cells. The correlation analysis of the MAPK signaling pathway-related DEGs and the differential microorganisms at the level of phylum showed that the </span></span><span><span>Firmicutes</span></span> was negatively correlated with these DEGs.</div></div><div><h3>Conclusion</h3><div>We profiled the characteristics of lung microbiota and immune landscape in PCP, which may contribute to elucidating the mechanism of PCP.</div></div>","PeriodicalId":18497,"journal":{"name":"Microbes and Infection","volume":"26 8","pages":"Article 105374"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141288308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0