Metabolites最新文献

{"title":"Investigating Systemic Metabolic Effects of <i>Betula alba</i> Leaf Extract in Rats via Urinary Metabolomics.","authors":"Gregorio Peron, Alina Yerkassymova, Gokhan Zengin, Stefano Dall'Acqua","doi":"10.3390/metabo15070471","DOIUrl":"https://doi.org/10.3390/metabo15070471","url":null,"abstract":"<p><strong>Background/objectives: </strong>Herbal extracts from <i>Betula alba</i> (birch) are traditionally used for their purported diuretic effects, but scientific evidence supporting these claims remains limited. In this pilot study, we evaluated the short-term effects of a standardized <i>B. alba</i> leaf extract in healthy adult rats using an untargeted urinary metabolomics approach based on UPLC-QTOF.</p><p><strong>Methods: </strong>Two doses, 25 or 50 mg/kg, of a standardized <i>B. alba</i> extract were orally administered to rats. The extract contains hyperoside (0.53%), quercetin glucuronide (0.36%), myricetin glucoside (0.32%), and chlorogenic acid (0.28%) as its main constituents. After 3 days of treatment, the 24 h urine output was measured.</p><p><strong>Results: </strong>While no statistically significant changes were observed in the 24 h urine volume or the urinary Na⁺ and K⁺ excretion, multivariate metabolomic analysis revealed treatment-induced alterations in the urinary metabolic profile. Notably, the levels of two glucocorticoids, i.e., corticosterone and 11-dehydrocorticosterone, were increased in treated animals, suggesting that the extract may influence corticosteroid metabolism or excretion, potentially impacting antidiuretic hormone signaling. Elevated bile-related compounds, including bile acids and bilin, and glucuronidated metabolites were also observed, indicating changes in bile acid metabolism, hepatic detoxification, and possibly gut microbiota activity.</p><p><strong>Conclusions: </strong>Although this study did not confirm a diuretic effect of <i>B. alba</i> extract, the observed metabolic shifts suggest broader systemic bioactivities that warrant further investigation. Overall, the results indicate that the approach based on urinary metabolomics may be valuable in uncovering the mechanisms of action and evaluating the bioactivity of herbal extracts with purported diuretic properties.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 7","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Adjuvant Effect of Hyperbaric Oxygenation for <i>Loxosceles rufescens</i> Bite: A Case Series.","authors":"Simona Mrakic-Sposta, Alessandra Vezzoli, Carmela Graci, Maristella Gussoni, Attilio Cimmino, Cinzia Dellanoce, Enrico Maria Camporesi, Giovanni Sesana, Gerardo Bosco","doi":"10.3390/metabo15070470","DOIUrl":"https://doi.org/10.3390/metabo15070470","url":null,"abstract":"<p><strong>Background: </strong>The venom of <i>Loxosceles</i><i>rufescens</i> (L.r.), also known as the violin and/or brown spider, contains a wide variety of proteins and can induce a complex, intense, and uncontrolled inflammatory response, hemolysis, thrombocytopenia, dermo-necrosis, and renal failure. Studies have postulated the efficacy of hyperbaric oxygen therapy (HBOT) for <i>Loxosceles</i> bites. However, data describing the use and beneficial effects of HBO are, to date, relatively scarce. Only a few cases of <i>Loxosceles</i> bites in Northern Italy have been documented, and there is no laboratory test available for the diagnosis.</p><p><strong>Objectives: </strong>We present seven cases (aged 54.5 ± 4.2 years) of patients who presented to the emergency room (E.R.) of Niguarda Hospital in Milan from March to October 2022.</p><p><strong>Methods: </strong>Blood and urine samples were collected and biomarkers of oxidative stress (OxS) (reactive oxygen species (ROS), total antioxidant capacity (TAC), lipid peroxidation (8iso-PFG2α), DNA damage (8-OH-dG)), inflammation (IL-6, IL-1β, TNF-α, sICAM1), and renal function (creatinine, neopterin, uric acid) before (T0), during (T1, T2), and after (1-2 wk T3-T4; 1 month T5) the HBOT treatment (US Navy Treatment Table 15 protocol) were studied.</p><p><strong>Results: </strong>At T0, patients showed a significant unbalance of OxS; high levels of ROS, 8-isoPGF2α, and inflammatory status (IL-6, TNF-α; sICAM); and a low level of antioxidant capacity. At the end of HBOT (T2), a significant reduction in Oxy-inflammation levels over time-8-iso -26%, 8-OH-dG -9%, IL-6 -71%, IL-1bβ -12%, TNF-α -13%, and sICAM1 -17%-associated with clinical improvement was shown.</p><p><strong>Conclusions: </strong>These reductions, along with those in renal function markers, mirrored the observed improvement in the evolution of the skin lesion and the patients' self-reported general wellness and pain. In conclusion, HBOT should be considered a valuable therapeutic tool after L.r. bites.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 7","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amide Proton Transfer-Weighted MR Imaging and Signal Variations in a Rat Model of Lipopolysaccharide-Induced Sepsis-Associated Encephalopathy.","authors":"Donghoon Lee, HyunJu Ryu, Yeon Ji Chae, Hind Binjaffar, Chul-Woong Woo, Dong-Cheol Woo, Do-Wan Lee","doi":"10.3390/metabo15070465","DOIUrl":"https://doi.org/10.3390/metabo15070465","url":null,"abstract":"<p><p><b>Introduction:</b> Sepsis-associated encephalopathy (SAE) is an acute brain dysfunction secondary to systemic infection, occurring without direct central nervous system involvement. Despite its clinical relevance, reliable biomarkers for diagnosing SAE and assessing its severity remain limited. This study aimed to evaluate the feasibility of amide proton transfer-weighted (APTw) chemical exchange saturation transfer (CEST) MRI as a non-invasive molecular imaging technique for detecting metabolic alterations related to neuroinflammation in SAE. Using a lipopolysaccharide (LPS)-induced rat model, we focused on hippocampal changes associated with neuronal inflammation. <b>Materials and Methods:</b> Twenty-one Sprague-Dawley rats (8 weeks old, male) were divided into three groups: control (CTRL, <i>n</i> = 7), LPS-induced sepsis at 5 mg/kg (LPS05, <i>n</i> = 7), and 10 mg/kg (LPS10, <i>n</i> = 7). Sepsis was induced via a single intraperitoneal injection of LPS. APTw imaging was performed using a 7 T preclinical MRI system, and signal quantification in the hippocampus was conducted using the magnetization transfer ratio asymmetry analysis. <b>Results and Discussion:</b> APTw imaging at 7 T demonstrated significantly elevated hippocampal APTw signals in SAE model rats (LPS05 and LPS10) compared to the control (CTRL) group: CTRL (-1.940 ± 0.207%) vs. LPS05 (-0.472 ± 0.485%) (<i>p</i> < 0.001) and CTRL vs. LPS10 (-0.491 ± 0.279%) (<i>p</i> < 0.001). However, no statistically significant difference was observed between the LPS05 and LPS10 groups (<i>p</i> = 0.994). These results suggest that APTw imaging can effectively detect neuroinflammation-related metabolic alterations in the hippocampus. <b>Conclusion:</b> Our findings support the feasibility of APTw CEST imaging as a non-invasive molecular MRI technique for SAE, with potential applications in diagnosis, disease monitoring, and therapeutic evaluation.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 7","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Avian Influenza Virus Strain Specificity in the Volatile Metabolome.","authors":"Young Eun Lee, Richard A Bowen, Bruce A Kimball","doi":"10.3390/metabo15070468","DOIUrl":"https://doi.org/10.3390/metabo15070468","url":null,"abstract":"<p><strong>Background/objectives: </strong>Outbreaks of highly pathogenic avian influenza virus (AIV) result in significant financial losses and the death or depopulation of millions of domestic birds. Early and rapid detection and surveillance are needed to slow the spread of AIV and prevent its spillover to humans. The volatile metabolome (i.e., the pattern of volatile metabolites emitted by a living subject) represents one such source of health information that can be monitored for disease diagnosis. Indeed, dogs have been successfully trained to recognize patterns of \"body odors\" associated with many diseases. Because little is known regarding the mechanisms involved in the alteration of the volatile metabolome in response to health perturbation, questions still arise regarding the specificity, or lack thereof, of these alterations.</p><p><strong>Methods: </strong>To address this concern, we experimentally infected twenty mallard ducks with one of two different strains of low-pathogenic AIV (ten ducks per strain) and collected cloacal swabs at various time points before and after infection.</p><p><strong>Results: </strong>Headspace analyses revealed that four volatiles were significantly altered following infection, with distinct profiles associated with each viral strain. The volatiles that differed between strains among post-infection sampling periods included ethylbenzyl ether (<i>p</i> = 0.00006), 2-phenoxyethanol (<i>p</i> = 0.00017), 2-hydroxybenzaldehyde (<i>p</i> = 0.00022), and 6-methyl-5-hepten-2-one (<i>p</i> = 0.00034).</p><p><strong>Conclusions: </strong>These findings underscore that AIV-induced changes to the volatile metabolome are strain-specific, emphasizing the need for disease-specific profiling in diagnostic development.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 7","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prediagnostic Plasma Metabolomic Profiles Using NMR for Exfoliation Glaucoma Among US Health Professionals.","authors":"Akiko Hanyuda, Oana A Zeleznik, Yoshihiko Raita, Danielle E Haslam, Qi Sun, Kazuno Negishi, Louis R Pasquale, Jessica Lasky-Su, Janey L Wiggs, Jae H Kang","doi":"10.3390/metabo15070469","DOIUrl":"https://doi.org/10.3390/metabo15070469","url":null,"abstract":"<p><strong>Background: </strong>Exfoliation glaucoma (XFG) represents a form of deleterious ocular aging of unclear etiology. We evaluated prediagnostic nuclear magnetic resonance (NMR)-based metabolites in relation to XFG risk, expanding on our prior findings of XFG-related metabotypes using liquid chromatography-mass spectrometry (LC-MS).</p><p><strong>Methods: </strong>We identified 217 XFG cases and 217 matched controls nested within three prospective health professional cohorts with plasma collected a mean 11.8 years before case identification. Plasma metabolites were analyzed using the targeted NMR Nightingale platform. Conditional logistic models and Metabolite Set Enrichment Analysis were performed. Multiple comparison issues were addressed using the number of effective tests (NEF) and false discovery rate (FDR).</p><p><strong>Results: </strong>Among 235 profiled metabolites, higher glucose was significantly associated with a lower risk of XFG (odds ratio (95%CI) = 0.42 (0.26, 0.7); NEF = 0.03). Among metabolite classes, lipoprotein subclasses and branched-chain amino acids were inversely associated, while relative lipoprotein lipid concentrations were adversely associated (FDR < 0.05).</p><p><strong>Conclusion: </strong>NMR profiling revealed that glucose, branched-chain amino acids, lipoprotein subclasses, and relative lipoprotein lipid concentrations may play important roles in XFG etiology.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 7","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amelioration of Metabolic Syndrome by Co-Administration of <i>Lactobacillus johnsonii</i> CRL1231 and Wheat Bran in Mice via Gut Microbiota and Metabolites Modulation.","authors":"Matias Russo, Antonela Marquez, Estefanía Andrada, Sebastián Torres, Arlette Santacruz, Roxana Medina, Paola Gauffin-Cano","doi":"10.3390/metabo15070466","DOIUrl":"https://doi.org/10.3390/metabo15070466","url":null,"abstract":"<p><strong>Background/objectives: </strong><i>Lactobacillus johnsonii</i> CRL1231 (<i>Lj</i> CRL1231) is a strain with feruloyl esterase (FE) activity that enhances ferulic acid (FA) release from wheat bran (WB) and has potential as a probiotic for metabolic syndrome (MS). Given the potential health benefits of FA and its microbial metabolites, this study aimed to evaluate the therapeutic effect of <i>Lj</i> CRL1231 co-administered with WB in a mouse model of metabolic syndrome (MS) induced by a high-fat diet (HFD).</p><p><strong>Methods: </strong>Mice were divided into three groups and fed for 14 weeks as follows: the Control group (standard diet), the MS group (HFD+WB), and the MS+Lj group (HFD+WB and <i>Lj</i> CRL1231-dose 10⁸ cells/day). Specifically, we analyzed the changes in the intestinal microbiota (IM), colonic FE activity, generation of FA-derived and fermentation metabolites, and metabolic and inflammatory parameters.</p><p><strong>Results: </strong>Improvements in the MS+Lj group compared to the MS group included the following: a-a 38% increase in colonic FE activity, leading to elevated levels of FA-derived metabolites (e.g., dihydroferulic, dihydroxyphenylpropionic, and hydroxyphenylpropionic acids); b-a significant shift in the IM composition, with a 3.4-fold decrease in Firmicutes and a 2.9-fold increase in Bacteroidetes; c-a decrease in harmful bacteria (<i>Desulfovibrio</i>) by 93%, and beneficial bacteria like <i>Bifidobacterium</i> increased significantly (6.58 log cells/g); d-a 33% increase in total SCFAs; e-a 26% reduction in the adiposity index; f-a 12% increase in HDL cholesterol and a 19% reduction in triglycerides; g-normalized glucose and insulin resulting in a 2-fold lower HOMA-IR index; h-an improved inflammatory profile by decreasing TNF-α, IFN-γ, and IL-6 (3-, 5-, and 2-fold, respectively) and increasing IL-10 by 2-fold; i-alleviation of liver damage by normalizing of transaminases AST (19.70 ± 2.97 U/L) and ALT (13.12 ± 0.88 U/L); j-evidence of reduced oxidative damage.</p><p><strong>Conclusions: </strong>The co-administration of <i>L. johnsonii</i> CRL1231 and WB exerts a synergistic effect in mitigating the features of MS in HFD-fed mice. This effect is mediated by modulation of the gut microbiota, increased release of bioactive FA-derived compounds, and restoration of metabolic and inflammatory homeostasis. This strategy represents a promising dietary approach for MS management through targeted microbiota-metabolite interactions.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 7","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Berberine as a Bioactive Alkaloid: Multi-Omics Perspectives on Its Role in Obesity Management.","authors":"Bartłomiej Zieniuk, Magdalena Pawełkowicz","doi":"10.3390/metabo15070467","DOIUrl":"https://doi.org/10.3390/metabo15070467","url":null,"abstract":"<p><p>Berberine, a bioactive isoquinoline alkaloid derived from medicinal plants such as <i>Berberis</i> and <i>Coptis</i> species, shows significant promise for managing obesity and associated metabolic disorders. This review synthesizes evidence on its modulation of AMP-activated protein kinase (AMPK) signaling, gut microbiota composition, lipid metabolism, and adipokine networks, elucidating how these actions converge to suppress adipogenesis and improve insulin sensitivity. Metabolomic profiling reveals critical shifts in bile acid metabolism, short-chain fatty acid production, and mitochondrial function. Recent studies also highlight berberine's anti-inflammatory effects and regulatory influence on glucose homeostasis. Despite its promise, challenges in oral bioavailability and drug interactions necessitate the development of advanced delivery strategies. We further discuss nanoformulations and multi-omics approaches, which integrate data from genomics, transcriptomics, proteomics, and metabolomics, provide new insights into berberine's mechanisms, and may guide personalized therapeutic applications. While promising, further studies are needed to validate these findings in humans and translate them into effective clinical strategies.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 7","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Coverage Profiling of Hydroxyl and Amino Compounds in Sauce-Flavor Baijiu Using Bromine Isotope Labeling and Ultra-High Performance Liquid Chromatography-High-Resolution Mass Spectrometry.","authors":"Zixuan Wang, Youlan Sun, Tiantian Chen, Lili Jiang, Yuhao Shang, Xiaolong You, Feng Hu, Di Yu, Xinyu Liu, Bo Wan, Chunxiu Hu, Guowang Xu","doi":"10.3390/metabo15070464","DOIUrl":"https://doi.org/10.3390/metabo15070464","url":null,"abstract":"<p><p><b>Background</b>: Hydroxyl and amino compounds play a significant role in defining the flavor and quality of sauce-flavor Baijiu, yet their comprehensive analysis remains challenging due to limitations in detection sensitivity. In this study, we developed a novel bromine isotope labeling approach combined with ultra-high performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) to achieve high-coverage profiling of these compounds in sauce-flavor Baijiu. <b>Methods</b>: The method employs 5-bromonicotinoyl chloride (BrNC) for rapid (30 s) and mild (room temperature) labeling of hydroxyl and amino functional groups, utilizing bromine's natural isotopic pattern (Δ<i>m</i>/<i>z</i> = 1.998 Da) for efficient screening. Annotation was performed hierarchically at five confidence levels by integrating retention time, accurate mass, and MS/MS spectra. <b>Results</b>: A total of 309 hydroxyl and amino compounds, including flavor substances (e.g., tyrosol and phenethyl alcohol) and bioactive compounds (e.g., 3-phenyllactic acid), were identified in sauce-flavor Baijiu. The method exhibited excellent analytical performance, with wide linearity (1-4 orders of magnitude), precision (RSD < 18.3%), and stability (RSD < 15% over 48 h). When applied to sauce-flavor Baijiu samples of different grades, distinct compositional patterns were observed: premium-grade products showed greater metabolite diversity and higher contents of bioactive compounds, whereas lower-grade samples exhibited elevated concentrations of acidic flavor compounds. <b>Conclusions</b>: These results demonstrate that the established method is efficient for the comprehensive analysis of hydroxyl and amino compounds in complex food matrices. The findings provide valuable insights for quality control and flavor modulation in sauce-flavor Baijiu production.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 7","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the Metabolic Effects of Surgically Induced Renal Ischemia in Humans: A Temporal Approach.","authors":"Bhargav Arimilli, Tyler A On, Vaishnavi S Srirama, Ye Yang, Gitanjali Asampille, Jeffrey R Brender, Murali C Krishna, Jessica Y Hseuh, Viraj P Chegu, Zachary Kozel, Sandeep Gurram, Mark W Ball, William Marston Linehan, Daniel R Crooks","doi":"10.3390/metabo15070462","DOIUrl":"https://doi.org/10.3390/metabo15070462","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Thousands of nephrectomies are performed annually in the United States, but the short-term metabolic effects of surgically induced renal ischemia remain unclear. The conventional metabolic markers used to characterize post-surgical renal function, such as creatinine and GFR, are measured in the serum but do not provide metabolic information about the renal parenchyma itself. We aimed to characterize the immediate metabolic effects of surgical ischemia on renal parenchyma within a temporal framework. <b>Methods</b>: Timed renal parenchyma biopsies were collected from eight patients undergoing nephrectomy for renal cell carcinoma both prior to and after ligation of the renal hilum. These samples were ground, extracted, and analyzed using nuclear magnetic resonance (NMR) spectroscopy to measure changes in lactate, succinate, glucose, alanine, and glycine levels. <b>Results</b>: Due to experimental limitations, we were only able to draw limited conclusions from three patients. Of the five remaining patients, all had significant increases in lactate and succinate levels as a function of time, though the degree to which these increases occurred varied between each patient. Glucose levels generally decreased in the renal parenchyma but did not necessarily correlate with lactate production, assuming all glucose underwent fermentation to lactate in a hypoxic environment. Alanine and glycine levels did not change in a predictable pattern across patients. <b>Conclusions</b>: There are significant changes in lactate, glucose and succinate levels within minutes of the onset of renal ischemia in human patients. The degree of change in the metabolites analyzed varied significantly between patients. The length of surgical ischemia must be considered during surgical procurement of tumor specimens for metabolomic analysis.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 7","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Long-Term Impact of Preterm Birth on Metabolic Bone Profile and Bone Mineral Density in Childhood.","authors":"Panagiota Markopoulou, Artemis Doulgeraki, Arsinoi Koutroumpa, Georgios Polyzois, Helen Athanasopoulou, Christina Kanaka-Gantenbein, Tania Siahanidou","doi":"10.3390/metabo15070463","DOIUrl":"https://doi.org/10.3390/metabo15070463","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Recent data on long-term consequences of prematurity on bone health are conflicting. The aim of this study was to assess the metabolic bone profile and bone mineral density (BMD) in prepubertal children born prematurely and to examine possible associations between bone health parameters and perinatal morbidity factors. <b>Methods</b>: This cross-sectional observational study included 144 children of mean (SD) age 10.9 (1.6) years: 49 children born very preterm (≤32 gestational weeks), 37 moderate-to-late preterm (32⁺¹ to 36⁺⁶ gestational weeks), and 58 born at term (controls). Serum levels of calcium/Ca, phosphorus/P, alkaline phosphatase/ALP, 25-hydroxyvitamin D/25(OH)D, bone formation markers (osteocalcin/OC, procollagen type I C-terminal propeptide/PICP, and insulin growth factor-1/IGF-1), and bone resorption markers (serum tartrate-resistant acid phosphatase 5b/bone TRAP5band urinary calcium-to-creatinine ratio) were measured. Total-body and lumbar-spine BMD and BMD Z-scores were calculated using dual-energy X-ray absorptiometry/DXA. <b>Results</b>: Children born very preterm showed significantly higher ALP, OC, PICP, and bone TRAP5b levels compared to controls, as well as compared to children born moderate-to-late preterm. Total-body and lumbar-spine BMD Z-scores were significantly lower in the very preterm-born group compared to controls. Gestational diabetes, preeclampsia, and bronchopulmonary dysplasia were associated with lower total-body BMD in the very preterm-born population. <b>Conclusions</b>: Preterm birth is associated with impaired metabolic bone profile and lower total-body and lumbar-spine BMD in childhood. Moderate-to-late preterm-born children exhibit altered metabolic bone parameters compared to very preterm-born children. Further research in children might allow better insight into the long-term impact of preterm birth on bone health.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 7","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144708117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}