Metabolites最新文献

{"title":"Systematic Review and Meta-Analysis of <i>Sclerocarya birrea</i> on Metabolic Disorders: Evidence from Preclinical Studies.","authors":"Desirée Victoria-Montesinos, Pura Ballester, Pablo Barcina-Pérez, Ana María García-Muñoz","doi":"10.3390/metabo14110615","DOIUrl":"10.3390/metabo14110615","url":null,"abstract":"<p><strong>Background/objectives: </strong>Metabolic disorders, including diabetes, obesity, and cardiovascular diseases, are significant global health issues. Nutraceuticals, such as <i>Sclerocarya birrea</i> (SB), known for its high polyphenol content, are increasingly explored for managing these conditions. This study aims to evaluate the antihyperglycemic, hypolipidemic, and antihypertensive effects of SB in animal models to understand its potential as a natural intervention for metabolic diseases.</p><p><strong>Methods: </strong>A systematic review and meta-analysis were conducted according to PRISMA guidelines. Searches across databases like PubMed, Web of Science, Embase, and Scopus identified studies using SB in animal models of metabolic disorders. Inclusion criteria were studies with SB intervention, control groups, and quantitative measures of metabolic parameters. The study was registered with INPLASY (INPLASY2024100031).</p><p><strong>Results: </strong>The meta-analysis revealed that SB significantly reduces blood glucose levels in diabetic animal models. Acute administration of SB showed a pooled standardized mean difference (SMD) of -7.13 (95% CI: -11.44 to -2.83) at 1 h and -9.75 (95% CI: -15.92 to -3.59) at 2-4 h post-administration. Chronic administration indicated a non-significant reduction in glucose levels (SMD: -5.69, 95% CI: -16.38 to 5.01).</p><p><strong>Conclusions: </strong>SB appears to have the potential for reducing blood glucose levels and may offer benefits for other cardiometabolic risk factors, including lipid profiles and oxidative stress. However, variability in the results underscores the need for further research, including standardized animal studies and clinical trials, to confirm these effects and clarify the mechanisms by which SB may impact metabolic disorders.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenolic Metabolites Protocatechuic Acid and Vanillic Acid Improve Nitric Oxide Bioavailability via the Akt-eNOS Pathway in Response to TNF-α Induced Oxidative Stress and Inflammation in Endothelial Cells.","authors":"Joseph Festa, Aamir Hussain, Zakia Al-Hareth, Stephen J Bailey, Harprit Singh, Mariasole Da Boit","doi":"10.3390/metabo14110613","DOIUrl":"10.3390/metabo14110613","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Reduced nitric oxide (NO) bioavailability secondary to excess-superoxide-driven oxidative stress is central to endothelial dysfunction. Previous studies suggest that phenolic metabolites may improve NO bioavailability, yet limited research is available in response to an inflammatory mediator. Therefore, we assessed the effects of cyanidin-3-glucoside (C3G) and its phenolic metabolites protocatechuic acid (PCA) and vanillic acid (VA) on NO bioavailability in a TNF-α induced inflammatory environment. <b>Methods</b>: Primary human umbilical vein endothelial cells (HUVECs) were supplemented with either C3G, PCA, or VA at 1 μM for 24 h before being stimulated with TNF-α 20 ng/mL for an additional 24 h. Measurements included cell viability, apoptosis, reactive oxygen species (ROS), nitrite concentrations, and endothelial nitric oxide synthase (eNOS) and Akt at the mRNA and protein level. <b>Results</b>: Phenolic metabolites did not increase the eNOS expression or nitrite levels in the unstimulated environment; rather, the metabolites mediated NO bioavailability in response to TNF-α induced oxidative stress, with increased viability, eNOS mRNA, phosphorylation, and nitrite levels. <b>Conclusions</b>: Phenolic metabolites, in the presence of TNF-α, can improve NO bioavailability at physiologically relevant concentrations via the Akt-eNOS pathway. This demonstrates that the induction of inflammation is a prerequisite for phenolic metabolites to promote protective properties in endothelial cells by activating the Akt-eNOS pathway.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596796/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Schinus terebinthifolia</i> Raddi-Untargeted Metabolomics Approach to Investigate the Chemical Variation in Volatile and Non-Volatile Compounds.","authors":"Mara Junqueira Carneiro, Guilherme Perez Pinheiro, Elisa Ribeiro Miranda Antunes, Leandro Wang Hantao, Thomas Moritz, Alexandra Christine Helena Frankland Sawaya","doi":"10.3390/metabo14110612","DOIUrl":"10.3390/metabo14110612","url":null,"abstract":"<p><strong>Context: </strong><i>Schinus terebinthifolia</i> Raddi is used in Brazilian folk medicine due to the wound healing and antiseptic properties of its bark, and its fruit are used as a condiment. However, the aerial parts of this plant have been studied and present some bioactive compounds as well.</p><p><strong>Objectives: </strong>The aim of this study was to investigate the variation in volatile and non-volatile composition of <i>S. terebinthifolia</i> leaves using untargeted metabolomics.</p><p><strong>Material and methods: </strong>The leaves of four trees were collected over one year; ethanolic extracts were analyzed by UHPLC-MS and fresh leaves were analyzed by GC-MS using HS-SPME. The data were processed using online software.</p><p><strong>Results: </strong>The results suggest seasonality interfered little with the chemical composition of leaves. On the other hand, the sex of the plant clearly determined the chemical composition of both volatile and non-volatile compounds.</p><p><strong>Discussion and conclusions: </strong>Chemical variability between plants with male and female flowers is fundamental information for the standardized use of its leaves. Compounds with important biological activities were putatively identified, confirming the potential use of <i>S. terebinthifolia</i> leaves as a source of bioactive compounds, reducing waste and increasing economic gains for local farmers throughout the year.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fermented Rice Bran Mitigated the Syndromes of Type 2 Diabetes in KK-<i>A^y</i> Mice Model.","authors":"Afifah Zahra Agista, Ami Kato, Tomoko Goto, Takuya Koseki, Akira Oikawa, Yusuke Ohsaki, Michiko Yamaki, Chiu-Li Yeh, Suh-Ching Yang, Ardiansyah, Slamet Budijanto, Michio Komai, Hitoshi Shirakawa","doi":"10.3390/metabo14110614","DOIUrl":"10.3390/metabo14110614","url":null,"abstract":"<p><p><b>Background:</b> Diabetes is a devastating disease that causes millions of deaths. Fermented rice bran (FRB), made by fermenting rice bran with <i>Aspergillus kawachii</i> and a mixture of lactic acid bacteria, was hypothesized to b able to improve diabetes-related symptoms. This study aimed to investigate the effects of FRB supplementation in mitigating type 2 diabetes symptoms and identifying FRB bioactive compounds. <b>Methods</b>: In this study, KK-<i>A^y</i> mice (4 w.o. male) were used as a model for type 2 diabetes. Mice were divided into three different groups. The first group received a control diet, the second received a 12.5% non-fermented rice bran (RB) supplemented diet, and the last group was fed a 12.5% FRB-supplemented diet. Supplementation was done for 4 weeks. <b>Results</b>: FRB supplementation lowered the blood glucose level, OGTT, HOMA-IR, total cholesterol, liver RAGE protein, and <i>glucokinase</i> in KK-<i>A^y</i> mice. Metabolome analysis of RB and FRB showed that fermentation increased bioactive compounds in rice bran, such as GABA, L-theanine, and carnitine. It also increased the levels of various free amino acids while converting some amino acids such as arginine, tyrosine, and tryptophan into other metabolites. <b>Conclusions</b>: This research showed the potency of FRB supplementation as a preventive agent against type 2 diabetes.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untargeted and Targeted Lipidomics Unveil Dynamic Lipid Metabolism Alterations in Type 2 Diabetes.","authors":"Li Feng, Bingshu He, Jianzhen Xia, Zhonghua Wang","doi":"10.3390/metabo14110610","DOIUrl":"10.3390/metabo14110610","url":null,"abstract":"<p><strong>Background: </strong>Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder with a growing body of evidence suggesting the central role of lipid metabolism in its pathogenesis. However, the dynamic changes in lipid metabolism across different stages of T2DM remain understudied.</p><p><strong>Objective: </strong>This study aimed to elucidate the temporal alterations in lipid metabolism in T2DM using an integrated lipidomics approach.</p><p><strong>Method: </strong>Serum samples from 155 subjects were analyzed using LC-MS-based lipidomics, including untargeted and targeted approaches.</p><p><strong>Results: </strong>We identified significant alterations in 44 lipid metabolites in newly diagnosed T2DM patients and 29 in high-risk individuals, compared with healthy controls. Key metabolic pathways such as sphingomyelin, phosphatidylcholine, and sterol ester metabolism were disrupted, highlighting the involvement of insulin resistance and oxidative stress in T2DM progression. Moreover, 13 lipid metabolites exhibited diagnostic potential for T2DN, showing consistent trends of increase or decrease as the disease progressed.</p><p><strong>Conclusion: </strong>Our findings underscore the importance of lipid metabolism in T2D development and identify potential lipid biomarkers for early diagnosis and monitoring of disease progression, which contribute to paving the way for novel therapeutic strategies.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Substance Use Disorder on Tryptophan Metabolism Through the Kynurenine Pathway: A Narrative Review.","authors":"Lindsey Contella, Christopher L Farrell, Luigi Boccuto, Alain H Litwin, Marion L Snyder","doi":"10.3390/metabo14110611","DOIUrl":"10.3390/metabo14110611","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Substance use disorder is a crisis impacting many people in the United States. This review aimed to identify the effect addictive substances have on the kynurenine pathway. Tryptophan is an essential amino acid metabolized by the serotonin and kynurenine pathways. The metabolites of these pathways play a role in the biological reward system. Addictive substances have been shown to cause imbalances in the ratios of these metabolites. With current treatment and therapeutic options being suboptimal, identifying biochemical mechanisms that are impacted during the use of addictive substances can provide alternative options for treatment or drug discovery. <b>Methods</b>: A systematic literature search was conducted to identify studies evaluating the relationship between substance use disorder and tryptophan metabolism through the kynurenine pathway. A total of 32 articles meeting eligibility criteria were used to review the relationship between the kynurenine pathway, tryptophan breakdown, and addictive substances. <b>Results:</b> The use of addictive substances dysregulates tryptophan metabolism and kynurenine metabolite concentrations. This imbalance directly affects the dopamine reward system and is thought to promote continued substance use. <b>Conclusions:</b> Further studies are needed to fully evaluate the metabolites of the kynurenine pathway, along with other options for treatment to repair the metabolite imbalance. Several possible therapeutics have been identified; drugs that restore homeostasis, such as Ro 61-8048 and natural products like <i>Tinospora cordifolia</i> or <i>Decaisnea insignis</i>, are promising options for the treatment of substance use disorder.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11597030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction Methods for Brain Biopsy NMR Metabolomics: Balancing Metabolite Stability and Protein Precipitation.","authors":"Wenzheng Xiong, Florian Zirpel, M Zameel Cader, Daniel C Anthony, Fay Probert","doi":"10.3390/metabo14110609","DOIUrl":"10.3390/metabo14110609","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Metabolic profiling of tissue samples via liquid-state nuclear magnetic resonance (NMR) requires the extraction of polar metabolites in a suitable deuterated solvent. Such methods often prioritise metabolite recovery over protein removal due to the relatively low sensitivity of NMR metabolomics and the routine use of methods able to supress residual protein signals. However, residual protein may impact metabolite integrity and the metabolite stability after NMR sample preparation is often overlooked. This study aimed to investigate the effect of residual protein contamination in rodent brain extracts and identify a reproducible extraction method that optimises metabolite recovery while ensuring sample stability. <b>Methods</b>: The performance of acetonitrile/water (50-100% MeCN), methanol/water (50-100% MeOH), and methanol/water/chloroform (MeOH/H₂O/CHCl₃) were assessed for extraction efficiency, reproducibility, residual protein contamination, and metabolite stability up to eight hours post NMR sample preparation. <b>Results</b>: Aspartate and glutamate deuteration were observed in 50% MeCN, 50% MeOH, and 67% MeOH extractions along with the conversion of N-acetyl aspartate to aspartate and acetate in 50% MeCN and 50% MeOH extractions. Both observations correlated with residual protein contamination and, thus, are a result of inadequate protein precipitation, as confirmed by ultrafiltration. MeOH/H₂O/CHCl₃ extraction preserved the stability of these metabolites while maintaining good extraction efficiency and reproducibility. <b>Conclusions</b>: Thus, we recommend MeOH/H₂O/CHCl₃ extraction for untargeted brain NMR metabolic profiling due to its effective protein precipitation and reliable performance. Nonetheless, the performance of detecting metabolites prone to oxidation such as ascorbate and glutathione is not improved by this method.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zic-HILIC MS/MS Method for NADomics Provides Novel Insights into Redox Homeostasis in <i>Escherichia coli</i> BL21 Under Microaerobic and Anaerobic Conditions.","authors":"Divyata Vilas Rane, Laura García-Calvo, Kåre Andre Kristiansen, Per Bruheim","doi":"10.3390/metabo14110607","DOIUrl":"10.3390/metabo14110607","url":null,"abstract":"<p><p><b>Background:</b> Nicotinamide adenine dinucleotide (NAD⁺), its precursors, and its derivatives (collectively NADome) play a crucial role in cellular processes and maintain redox homeostasis. Understanding the dynamics of these metabolic pools and redox reactions can provide valuable insights into metabolic functions, especially cellular regulation and stress response mechanisms. The accurate quantification of these metabolites is challenging due to the interconversion between the redox forms. <b>Methods:</b> Our laboratory previously developed a zwitterionic hydrophilic interaction liquid chromatography (zic-HILIC)-tandem mass spectrometry method for the quantification of five essential pyridine nucleotides, including NAD⁺ derivatives and it's reduced forms, with ¹³C isotope dilution and matrix-matched calibration. In this study, we have improved the performance of the chromatographic method and expanded its scope to twelve analytes for a comprehensive view of NAD⁺ biosynthesis and utilization. The analytical method was validated and applied to investigate <i>Escherichia coli</i> BL21 under varying oxygen supplies including aerobic, microaerobic, and anaerobic conditions. <b>Conclusions</b>: The intracellular absolute metabolite concentrations ranged over four orders of magnitude with NAD⁺ as the highest abundant, while its precursors were much less abundant. The composition of the NADome at oxygen-limited conditions aligned more with that in the anaerobic conditions rather than in the aerobic phase. Overall, the NADome was quite homeostatic and <i>E. coli</i> rapidly, but in a minor way, adapted the metabolic activity to the challenging shift in the growth conditions and achieved redox balance. Our findings demonstrate that the zic-HILIC-MS/MS method is sensitive, accurate, robust, and high-throughput, providing valuable insights into NAD⁺ metabolism and the potential significance of these metabolites in various biological contexts.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596675/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvements in Insulin Resistance and Glucose Metabolism Related to Breastfeeding Are Not Mediated by Subclinical Inflammation.","authors":"Julia Martins de Oliveira, Patrícia Médici Dualib, Alexandre Archanjo Ferraro, Rosiane Mattar, Sérgio Atala Dib, Bianca de Almeida-Pititto","doi":"10.3390/metabo14110608","DOIUrl":"10.3390/metabo14110608","url":null,"abstract":"<p><p><b>Background:</b> Lactation is known to improve insulin resistance, but this phenomenon remains poorly understood. Our goal was to evaluate whether subclinical inflammation could mediate the association between breastfeeding (BF) and improvement in glucose metabolism and markers of insulin resistance (MIRs) in the postpartum. <b>Methods:</b> A total of 95 adult women (≥18 years) with a BMI ≥ 25 kg/m² from the outpatient clinic of the Federal University of São Paulo were followed from early pregnancy until 60 to 180 days postpartum. The patients were divided based on their BF status: BF and non-BF groups. A latent variable termed SubInf was created incorporating inflammation-related biomarkers: adiponectin, E-selectin, branched-chain amino acids, zonulin, copeptin, and lipopolysaccharides. The association of BR with MIRs in the postpartum was evaluated through linear regression analysis, and mediation analysis was performed to evaluate the role of SubInf in this association. <b>Results:</b> The groups were similar regarding gestational diabetes mellitus (GDM) prevalence, pre-gestational BMI, caloric intake, physical activity, and postpartum weight loss. The BF group presented lower levels of triglycerides (TGs), fasting glucose, fasting insulin, TG/HDLcholesterol ratio (TG/HDL), TyG index, and HOMA-IR compared to the non-BF group. A linear regression analysis adjusted for scholarity, parity, pre-gestational BMI, GDM, weight gain during pregnancy, and mode of delivery revealed an inverse association between BF and fasting glucose [-6.30 (-10.71 to -1.89), <i>p</i> = 0.005), HOMA-IR [-0.28 (-0.50 to -0.05), <i>p</i> = 0.017], TyG index [-0.04 (-0.06 to -0.01), <i>p</i> = 0.002], and TG/HDL ratio [-0.23 (-0.46 to -0.01), <i>p</i> = 0.001]. In the mediation analysis, SubInf did not mediate the indirect effect of BF on MIRs. <b>Conclusions:</b> In overweight and obese women, an association between BF and improvement in MIRs in the postpartum was seen, corroborating that BF should be stimulated, especially in these cardiometabolic high-risk women. Subclinical inflammation did not seem to mediate this association.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596560/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling of Metabolome in the Plasma Following a circH19 Knockdown Intervention in Diet-Induced Obese Mice.","authors":"Hanxin Zhao, Dike Shi, Weiwei Gui, Xihua Lin, Jionghuang Chen, Weihua Yu","doi":"10.3390/metabo14110603","DOIUrl":"10.3390/metabo14110603","url":null,"abstract":"<p><p>The circular RNA circH19 has been implicated in the regulation of gene expression and various biological processes, including obesity. <b>Objectives</b>: This study aimed to elucidate the metabolic changes in plasma after circH19 knockdown in a diet-induced obese (DIO) mouse model. <b>Methods:</b> Plasma samples were collected following the intervention and subjected to non-targeted metabolomics analysis using liquid chromatography-mass spectrometry (LC-MS). Metabolic profiling was performed to identify and quantify metabolites, followed by multivariate statistical analysis to discern differential metabolic signatures. <b>Results:</b> A total of 1250 features were quantified, resulting in the upregulation of 564 metabolites and the downregulation of 686 metabolites in the circH19 knockdown group compared to the control mice. Metabolic pathway analysis revealed disruptions in lipid metabolism, amino acid turnover, and energy production pathways. Notably, the intervention led to a substantial decrease in circulating lipids and alterations in the plasma amino acid profile, indicative of an impact on protein catabolism and anabolic processes. The observed shifts in lipid and amino acid metabolism suggest potential therapeutic avenues for obesity and related metabolic disorders. <b>Conclusions:</b> The circH19 knockdown in DIO mice led to significant alterations in plasma metabolites, highlighting its potential role in the regulation of obesity and metabolic disorders.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"14 11","pages":""},"PeriodicalIF":3.4,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11596236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142730207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}