Metabolites最新文献

{"title":"Zhenban Rootstock Optimizes Shatian Pomelo Fruit Quality by Coordinating Primary and Secondary Metabolism.","authors":"Xueyu Cui, Yujiao Peng, Zhuoming Liang, Xiao Du, Wenwen Huang, Lin Lin, Xiaoyu Zhang, Bing Wang, Chen Liu, Xiliang Xu","doi":"10.3390/metabo16090697","DOIUrl":"10.3390/metabo16090697","url":null,"abstract":"<p><strong>Background/objectives: </strong>Rootstock application is a critical strategy for improving citrus fruit quality, yet conventional combinations frequently suffer from an inherent sugar and acid imbalance. This study aimed to comprehensively evaluate the physiological and molecular impacts of three diverse rootstocks, including Trifoliate orange (Pt), Sour pomelo (Cg), and Zhenban pomelo (Zb), on Shatian pomelo to identify optimal grafting combinations and elucidate the underlying mechanisms governing superior fruit quality.</p><p><strong>Methods: </strong>Shatian pomelo scions were grafted onto three distinct rootstocks (Pt, Cg, and Zb) and compared against self-rooted Shatian pomelo plants (CK) under identical field conditions. We conducted a comparative physiological evaluation of specific fruit traits, explicitly examining single fruit weight, total soluble solids, dry matter content, as well as the dynamic accumulation of individual sugars, citric acid, and ascorbic acid. To uncover the underlying molecular regulatory networks, we integrated comprehensive transcriptomic and metabolomic analyses of the developing fruits, mapping the precise gene expression profiles and metabolite fluxes across the different treatments.</p><p><strong>Results: </strong>While the Pt and Cg rootstocks induced nonspecific metabolic fluctuations, the Zb rootstock uniquely optimized internal fruit quality without compromising fundamental fruit morphology. Zb grafted fruits exhibited superior sweetness, significantly reduced citric acid, and massively elevated ascorbic acid content. Integrative multiomics data revealed that Zb orchestrates a highly precise resource reallocation strategy. By transcriptionally suppressing energy-consuming pathways, specifically glycolysis and broad flavonoid biosynthesis, the Zb rootstock efficiently conserved vital structural carbon skeletons. This redirected carbon flux was seamlessly coordinated with the robust upregulation of SWEET sugar transporters and key enzymes in the L galactose pathway, thereby maximizing carbohydrate sink strength and vitamin C production. Concurrently, the downregulation of specific degradation enzymes ensured the stability of these targeted nutritional pools.</p><p><strong>Conclusions: </strong>The targeted carbon reallocation driven by the Zb rootstock effectively overcomes the traditional flavor bottlenecks in pomelo cultivation. These mechanistic insights not only highlight a highly efficient agricultural strategy for citrus improvement but also advance our fundamental understanding of metabolic regulation in grafted plants.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"16 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609140/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite-Related Evidence Linking Metabolic Measures to Patient-Reported Outcomes in Inflammatory Bowel Disease: A Critical Narrative Review.","authors":"Patrycja Krynicka, Aleksandra Helena Piotrowska, Maria Kłopocka","doi":"10.3390/metabo16090696","DOIUrl":"10.3390/metabo16090696","url":null,"abstract":"<p><strong>Background/objectives: </strong>Patient-reported outcomes (PROs) capture important dimensions of inflammatory bowel disease (IBD), including fatigue, questionnaire-defined mood-related symptom burden, pain, sleep disturbance, bowel urgency and impaired health-related quality of life, yet these outcomes are incompletely explained by conventional inflammatory markers. Metabolite-related measures may capture host-microbe co-metabolism relevant to patient-reported burden.</p><p><strong>Methods: </strong>This critical narrative review synthesized adult IBD literature linking metabolite-related measures with patient-reported symptom domains. PubMed/MEDLINE, Embase.com, Scopus and Web of Science Core Collection were searched through 11 June 2026; purposive source selection supported critical narrative synthesis rather than exhaustive enumeration.</p><p><strong>Results: </strong>Our structured search identified only a small number of human studies, mainly in fatigue and questionnaire-defined mood-related symptom burden. Studies included serum, plasma or fecal metabolite measurements, exploratory multi-omics, inferred microbial metabolic functions and interventional pathway testing. Fatigue was associated with serum amino-acid-related changes, plasma lipidomic alterations and fecal untargeted metabolomic and microbiome differences; inferred microbial functional modules were also reported. Mood-related burden was associated with serum and fecal metabolomic changes, bile-acid profiles and microbiota-derived functional signals. Within our search and eligibility criteria, no human studies paired measured metabolites with sleep-, pain- or urgency-specific patient-reported outcome measures (PROMs); evidence for multidimensional clusters was insufficient.</p><p><strong>Conclusions: </strong>We did not identify a metabolite-related signature for the assessment of patient-reported burden that had undergone both analytical and clinical validation; findings support candidate associations and exploratory signatures. Future work requires longitudinal, phenotype-stratified cohorts, validated PROMs, rigorous metabolomics methodology, causal modeling, inflammatory and dietary metadata, multiple-testing control and independent validation for clinical translation.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"16 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13608916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early Hepatic Transcriptomic Responses to High-Fat Diet in Apolipoprotein A-IV Knockout Mice.","authors":"Natalia Zeber-Lubecka, Maria Kulecka, Kazimiera Pyśniak, Michalina Dąbrowska, Ewa E Hennig","doi":"10.3390/metabo16090695","DOIUrl":"10.3390/metabo16090695","url":null,"abstract":"<p><strong>Background: </strong>Apolipoprotein A-IV (ApoA-IV) is involved in lipid metabolism and energy homeostasis, but its role in hepatic adaptation to dietary stress remains incompletely understood. This study aimed to determine whether ApoA-IV deficiency alters hepatic transcriptional responses to high-fat diet (HFD) and to examine how these molecular changes relate to biochemical and histological features of liver function.</p><p><strong>Methods: </strong>Male ApoA-IV knockout (KO) and wild-type (WT) mice were fed either a normal diet (ND) or HFD for 12 weeks. Liver tissues were subjected to whole-transcriptome RNA sequencing, followed by differential expression and functional enrichment analyses.</p><p><strong>Results: </strong>Histological examination revealed persistent lobular and portal inflammation accompanied by mild fibrosis in ApoA-IV-KO mice irrespective of diet, whereas steatosis and hepatocellular ballooning were absent in all groups. Both dietary intervention and genotype were associated with transcriptomic and biochemical alterations, although the identified changes were primarily evident at the level of specific genes and biological pathways. ApoA-IV deficiency was associated with a more limited set of diet-related transcriptomic changes, reflected by fewer differentially expressed genes and enriched functional categories compared to WT mice. Functional enrichment analyses identified alterations predominantly related to lipid and sterol metabolism, including the statin pathway, triglyceride metabolism, PPAR signaling and cytochrome P450-associated processes. Notably, the statin pathway was the only functional category consistently distinguishing ApoA-IV-deficient and WT mice regardless of diet. Additionally, HFD-fed ApoA-IV-KO mice exhibited elevated serum triglyceride, alanine aminotransferase and insulin levels, in contrast to WT mice.</p><p><strong>Conclusions: </strong>ApoA-IV deficiency was associated with the modulation of selected metabolic and inflammatory pathways involved in hepatic responses to dietary challenge. Cholesterol- and sterol-related pathways, including the statin pathway, represented the most consistent genotype-associated transcriptomic signature. Within the context of the present model, the findings suggest that ApoA-IV may contribute to hepatic responses during early stages of metabolic adaptation to dietary stress, although the biological significance of the observed transcriptomic differences requires further investigation.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"16 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Replacing Fish Meal with Poultry By-Product Meal on Growth, Physiological Status, Disease Resistance, and Hepatic Transcriptomic Responses in Hybrid Yellow Catfish (<i>Pelteobagrus fulvidraco</i> ♀ × <i>Pelteobagrus vachelli</i> ♂).","authors":"Peng Shi, Lei Wang, Xuxiong Huang, Biao Yun, Xueqiao Qian","doi":"10.3390/metabo16090694","DOIUrl":"10.3390/metabo16090694","url":null,"abstract":"<p><strong>Purpose: </strong>This study evaluated the effects of replacing dietary fish meal (FM) with poultry by-product meal (PBM) on growth, physiological status, disease resistance, and hepatic transcriptomic responses in hybrid yellow catfish (<i>Pelteobagrus fulvidraco</i> ♀ × <i>Pelteobagrus vachelli</i> ♂).</p><p><strong>Methods: </strong>Six isonitrogenous and isolipidic diets were formulated by replacing 0%, 12%, 24%, 36%, 48%, or 60% of dietary FM with PBM. Juveniles (initial weight 5.18 ± 0.02 g) were fed the experimental diets for 8 weeks.</p><p><strong>Results: </strong>Weight gain was maximized and the feed conversion ratio minimized in fish fed the FM19 diet, with broken-line regression analysis of weight gain estimating the optimal FM replacement level at 27.4%. Hepatic and intestinal superoxide dismutase activities, as well as intestinal trypsin activity, tended to decline as the level of FM replacement with PBM increased. Following <i>Edwardsiella ictaluri</i> challenge, 96-h cumulative survival was lower in the FM10 group than in all other groups. Principal component analysis of hepatic transcriptomes clearly separated the FM10 group from the FM25 and FM19 groups. Gene Ontology analysis associated differentially expressed genes with responses to hormones and endogenous stimuli (FM10 vs. FM25), protein folding and chaperone-mediated complex assembly (FM19 vs. FM25), and catabolic processes and mitochondrial function (FM10 vs. FM19). Kyoto Encyclopedia of Genes and Genomes analysis identified pathways involving mitogen-activated protein kinase signaling, protein processing in the endoplasmic reticulum, autophagy, apoptosis, and inflammatory responses. Moreover, genes associated with oxidative stress and inflammation showed higher expression in the FM10 group compared with the FM19 and FM25 groups.</p><p><strong>Conclusions: </strong>Moderate FM replacement with PBM improved growth and feed utilization, whereas excessive replacement was associated with lower antioxidant and digestive enzyme activities, altered metabolic responses, and enhanced expression of inflammation-related genes, supporting PBM as a partial FM substitute in low-FM diets for hybrid yellow catfish.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"16 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organ-Resolved Metabolomics of <i>Cibotium barometz</i> Reveals Rhizome-Specific Terpenoid Lactones and Phenolic-Rich Non-Officinal Organs.","authors":"Guole Qin, Daizu Xie, Changqin Zhou, Delong Guan, Jing Song","doi":"10.3390/metabo16090693","DOIUrl":"10.3390/metabo16090693","url":null,"abstract":"<p><strong>Background: </strong><i>Cibotium barometz</i> (L.) J. Sm. is a medicinal fern whose rhizome is the sole officinal part, while roots, stems, leaves and young shoots are discarded as agricultural residues. The chemical rationale for this restriction, and the value of the discarded organs, remain undefined.</p><p><strong>Materials and methods: </strong>Here, untargeted LC-MS/MS metabolomics (UHPLC-Orbitrap) of methanol/acetonitrile/water extracts was applied to five organs of <i>C. barometz</i> (three pooled biological replicates per organ), annotating 2765 polar-to-moderately-polar metabolites.</p><p><strong>Results: </strong>Organ identity dominated the metabolic architecture of the sampled population: the first two principal components separated all five organs, with leaf and root as the two mutually opposed metabolic extremes and stem, young shoot and rhizome forming a contiguous module. Exploratory pairwise OPLS-DA combined with FDR-controlled univariate criteria yielded 193-1223 differentially accumulated metabolites per comparison, and tissue-specificity analysis assigned 2031 metabolites (73.45%) predominantly to a single organ.</p><p><strong>Discussion: </strong>Metabolite accumulation patterns along the phenylpropanoid-flavonoid pathway was spatially partitioned, with flavan-3-ols and isoflavones accumulating in root, flavone/flavonol glycosides in leaf and predominantly hydroxycinnamic acids in rhizome and young shoot. Of 22 previously reported or candidate quality control constituents, putatively annotated terpenoid lactones were detected almost exclusively in the rhizome, whereas several phenolics showed markedly higher relative abundance in root or leaf.</p><p><strong>Conclusions: </strong>These relative-abundance data indicate that the non-officinal organs are chemically complementary potential resources; absolute quantification, bioactivity and safety evaluation are prerequisites before any medicinal valorisation.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"16 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exercise Metabolomics Reveals Intensity-Dependent Metabolic Responses Associated with Cardiorespiratory Fitness in Adults with Type 1 Diabetes.","authors":"Olivia M McCarthy, Clara Solà, Chloe Nicholas, Sandra Tawfik, Merete Bechmann Christensen, Signe Schmidt, Kirsten Nørgaard, Richard M Bracken, Marietta Kokla","doi":"10.3390/metabo16090687","DOIUrl":"10.3390/metabo16090687","url":null,"abstract":"<p><p><b>Purpose</b>: To characterize plasma metabolomic responses to maximal exercise and their associations with cardiorespiratory fitness (CRF) in adults with type 1 diabetes. <b>Methods</b>: In this exploratory pooled secondary analysis, 21 adults (67% female; age, 51.6 ± 13.2 years; HbA1c, 67.5 ± 7.0 mmol/mol) completed a cycle-ergometer test to exhaustion; metabolomic data were available for 20. Plasma collected at rest, anaerobic threshold (AT), peak exercise, and recovery underwent targeted and untargeted gas chromatography-mass spectrometry profiling. Peak oxygen uptake (V̇O₂peak) quantified CRF. Linear mixed-effects models adjusted for age, sex, HbA1c, and diabetes duration. <b>Results</b>: At the AT, L-(+)-lactic acid and 2-hydroxyisobutyric acid increased relative to rest (both <i>q</i> ≤ 0.010). Peak exercise produced changes in glycolytic, tricarboxylic acid (TCA)-cycle, and purine metabolites, with enrichment of the TCA cycle and nucleotide metabolism. TCA-cycle enrichment persisted during recovery. In exploratory interaction analyses, higher V̇O_2peak was associated with increases in L-(+)-lactic acid at AT (β = 0.032, <i>q</i> = 0.040) and peak exercise (β = 0.031, <i>q</i> = 0.024), and in malic acid at peak exercise (β = 0.063, <i>q</i> = 0.010) and recovery (β = 0.061, <i>q</i> = 0.015). <b>Conclusions</b>: These preliminary findings suggest the presence of intensity-dependent and fitness-associated metabolic responses to exercise in adults with type 1 diabetes. Given the small sample size, particularly for interaction analyses, these findings require confirmation in larger independent cohorts.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"16 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13608913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incremental Prognostic Value of Albumin-Anchored Inflammatory Ratios Beyond a SOFA-Based Model in a Medical ICU: A Retrospective Cohort Study.","authors":"Özkul Yılmaz Çolak, Melda İşevi, Tuğçehan Sezer Akman, Özgür Kılıç, Neslihan Ünal Akdemir","doi":"10.3390/metabo16090692","DOIUrl":"10.3390/metabo16090692","url":null,"abstract":"<p><p><b>Background/Objectives:</b> We compared three albumin-anchored ratios calculated from measurements obtained during the first 24 h, C-reactive protein (CRP)-to-albumin (CAR), procalcitonin-to-albumin (PAR) and lactate-to-albumin (LAR), as predictors of intensive care unit (ICU) mortality beyond an established severity model. <b>Methods:</b> Eligibility required an ICU stay reaching 24 h and a complete early work-up; 372 of 1352 screened admissions qualified, with each ratio using the worst component values in that window. Incremental value over a base model (Sequential Organ Failure Assessment (SOFA) score, sex, age-adjusted Charlson index) was assessed by change in the area under the curve (AUC; DeLong test), reclassification metrics (NRI, IDI) and calibration, with alternative baselines and outcomes, multiplicity adjustment, and selection weights from the excluded admissions. <b>Results:</b> Of 372 patients, 248 (66.7%) died. As standalone predictors, CAR (0.671), PAR (0.689) and LAR (0.642) were indistinguishable and inferior to SOFA (0.730). Only CAR significantly improved AUC discrimination (ΔAUC +0.030, <i>p</i> = 0.024; NRI +0.39; IDI +0.042); PAR and LAR did not significantly improve AUC discrimination. Entered together, CAR remained independent (aOR 1.52, 1.15-2.03), while PAR did not (1.20, 0.89-1.63). The cohort was sicker than the 626 patients excluded for incomplete work-up; after weighting, CAR stayed independent (aOR 1.47) but ΔAUC fell to +0.018. The increment was significant against SOFA alone (+0.033) but not against baselines including admission diagnosis or organ support; it remained significant after Benjamini-Hochberg but not Bonferroni adjustment. With in-hospital mortality it was larger (+0.044), but PAR then also retained independence. <b>Conclusions:</b> The first 24 h CAR provided a well calibrated improvement beyond a parsimonious SOFA-based model, but the increment was small and not robust to richer baselines or to multiplicity correction, and no subgroup-specific claim is supported. These hypothesis-generating findings need prospective validation.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"16 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609766/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Volatile Metabolite Profiling and Bioactive Marker Discovery of Saposhnikoviae Radix via HS-GC-MS/MS Integrated with Chemometrics and Network Pharmacology.","authors":"Fangliang He, Xianrui Wang, Jiating Zhang, Lizhi Wan, Haonan Wu, Xianlong Cheng, Jia Chen, Xiangri Li, Wenguang Jing, Feng Wei","doi":"10.3390/metabo16090689","DOIUrl":"10.3390/metabo16090689","url":null,"abstract":"<p><p><b>Background:</b> Saposhnikoviae radix (SR), a valuable Chinese medicinal plant with high medicinal and edible value, suffers from inconsistent quality caused by growth patterns, cultivation duration, and geographical origin. <b>Methods:</b> In this study, comprehensive volatile metabolite profiling using HS-GC-MS/MS combined with multivariate chemometrics was performed to characterize these quality-related differences. <b>Results:</b> In total, 7, 13, and 11 differential volatile compounds were identified for wild versus cultivated SR, cultivation duration, and geographical origin, respectively. By integrating the three comparisons, 11 recurrent differential compounds were selected for network pharmacology and molecular docking analyses. trans-3-Nonen-2-one, benzaldehyde, and Octanoic acid were predicted to interact with key targets, including TNF, IL1B, ALB, EGFR, and CASP3, which were mainly enriched in inflammation- and pain-related pathways, including the PI3K-Akt signaling pathway, apoptosis, and the HIF-1 signaling pathway. Molecular docking further supported favorable predicted interactions between the selected compounds and core targets. <b>Conclusions:</b> Overall, this study reveals volatile metabolite variation associated with major quality-related factors of SR and identifies candidate bioactive and quality-associated volatile compounds. The integration of volatile metabolite profiling, chemometrics, network pharmacology, and molecular docking provides a complementary strategy for the comprehensive quality evaluation of SR and offers a reliable reference for quality control research on other medicinal Apiaceae species.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"16 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609242/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute Responses to Creatine Monohydrate and L-Arginine, Alone and Combined, on Repeated-Sprint Power, Countermovement Jump Height, and Stroop Reaction Time in Recreationally Active Men: A Randomized Double-Blind Crossover Trial.","authors":"Özgür Eken, Halil Uçar, Ahmet Kurtoğlu, Murat Ozan, Yusuf Buzdağlı, Mehmet Söyler, Monira I Aldhahi","doi":"10.3390/metabo16090688","DOIUrl":"10.3390/metabo16090688","url":null,"abstract":"<p><strong>Background/objectives: </strong>Creatine monohydrate (CR) and L-arginine (LA) are widely used ergogenic supplements, but whether acute co-ingestion provides additional performance or task-specific cognitive benefits beyond either supplement alone remains uncertain. This study compared placebo, CR, LA, and LA+CR on repeated-sprint power, countermovement jump (CMJ) height, and Stroop reaction time (RT).</p><p><strong>Methods: </strong>In this randomized, double-blind, four-period crossover trial, 18 recreationally active men completed placebo (CG), CR (0.3 g·kg^-1), LA (6 g), and LA+CR conditions separated by at least 72 h (prospectively registered at ClinicalTrials.gov: NCT07548541). Testing began 60 min after ingestion with the Stroop test, followed by CMJ and the Running-Based Anaerobic Sprint Test (RAST). Co-primary outcomes were RAST average power (AP), peak power (PP), CMJ height, and incongruent Stroop median RT.</p><p><strong>Results: </strong>Condition effects were observed for AP, PP, minimum power, CMJ height, and Stroop RT (all <i>p</i> ≤ 0.011), but not fatigue index or Stroop accuracy. All four co-primary omnibus effects remained significant after Holm correction (all pHolm < 0.001). LA+CR produced higher PP than CG (MD 99.01 W; <i>p</i> < 0.001), LA (MD 51.29 W; <i>p</i> = 0.002), and CR (MD 46.71 W; <i>p</i> < 0.001), and greater CMJ height than all comparison conditions (all <i>p</i> < 0.001). LA+CR also produced lower Stroop RT than CG (MD 0.089 s; <i>p</i> < 0.001) and CR (<i>p</i> = 0.041), but not LA (<i>p</i> = 0.271). For AP, LA+CR exceeded CG and CR, but not LA after Bonferroni adjustment. In a secondary 2 × 2 factorial decomposition, no LA×CR interaction for a co-primary outcome survived Holm correction (all pHolm ≥ 0.104).</p><p><strong>Conclusions: </strong>Acute LA+CR produced outcome-specific improvements in peak sprint power and CMJ and selectively reduced Stroop RT, but did not establish biological synergy. Interpretation should remain exploratory given the small male-only sample, unmodelled period/sequence effects, and possible creatine-related carryover.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"16 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13609322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferroptosis and Ferro-Aging in Obesity: Bibliometric Mapping, Shared Mechanisms, and Translational Perspectives.","authors":"Jiaxin Liu, Likun Zheng, Zhengri Cong, Zehao Liu, Chen Hu, Yuxin Liu, Chong Zhang, Mingjun Liu","doi":"10.3390/metabo16090691","DOIUrl":"10.3390/metabo16090691","url":null,"abstract":"<p><p><b>Background</b>: Obesity is a chronic metabolic disease characterized by lipid overload, low-grade inflammation, oxidative stress, and progressive organ dysfunction. Ferroptosis, an iron-dependent form of regulated cell death driven by lipid peroxidation, has been increasingly implicated in obesity-related complications. However, an acute cell death-centered model does not fully explain the chronic senescence-like decline observed in metabolic tissues. The recently proposed concept of ferro-aging provides a potential framework for linking iron-dependent lipid peroxidation to cellular senescence and metabolic aging. <b>Methods</b>: In this review, we combined bibliometric mapping with mechanistic synthesis to characterize the evolving research landscape of ferroptosis in obesity and integrate the emerging concept of ferro-aging into this field. Bibliometric analysis was performed to screen qualified literature, and keyword co-occurrence and co-citation analyses were conducted to extract mainstream research hotspots. We further systematically summarized the pathological microenvironment constructed by obesity and the core molecular mediator connecting ferroptosis and ferro-aging, and proposed a pathological continuum hypothesis for obesity-mediated lipid peroxidation injury. <b>Results</b>: A total of 564 eligible publications were identified, including 455 original articles and 109 reviews, with a marked increase in publication output after 2020. Keyword and co-citation analyses highlighted oxidative stress, lipid peroxidation, iron metabolism, GPX4/Nrf2-mediated antioxidant defense, mitochondrial dysfunction, gut microbiota, NAFLD, and DCM as major research themes. Mechanistically, obesity creates a pro-ferroperoxidative microenvironment through iron dysregulation, polyunsaturated fatty acid enrichment, chronic inflammation, mitochondrial stress, and impaired antioxidant capacity. Within this context, ACSL4 is proposed as a candidate molecular hub that may link acute ferroptotic injury with chronic iron-lipid peroxidation-driven cellular senescence. We proposed a hypothesis-generating framework. In this framework, acute lipid peroxidation may promote ferroptotic cell death. Persistent sublethal iron-lipid peroxidation stress may contribute to ferro-aging-like senescence. <b>Conclusions</b>: To our knowledge, this is the first review to place ferro-aging within the obesity-ferroptosis framework. Targeting the ACSL4-lipid peroxidation axis and restoring antioxidant defense, particularly through Nrf2-GPX4-related pathways, may provide new translational opportunities for stratifying and managing obesity-related complications. However, clinical validation of ferro-aging biomarkers and intervention strategies remains urgently needed.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"16 9","pages":""},"PeriodicalIF":4.5,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13608923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149039445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}