Metabolites最新文献

{"title":"Quality Evaluation of the Traditional Chinese Medicine Moutan Cortex Based on UPLC Fingerprinting and Chemometrics Analysis.","authors":"Wentao Fang, Qianqian Song, Han Luo, Rui Wang, Chengwu Fang","doi":"10.3390/metabo15040281","DOIUrl":"https://doi.org/10.3390/metabo15040281","url":null,"abstract":"<p><strong>Background: </strong>This study aims to develop a fingerprint analysis method using ultra-high performance liquid chromatography (UPLC) for Moutan Cortex sourced from different regions. The objective is to establish quality control standards validated through the integration of chemometric methods and component structure theory.</p><p><strong>Methods: </strong>The mobile phase for UPLC consisted of acetonitrile (A) and a 0.1% aqueous formic acid solution (B), with gradient elution set as follows: 0-1 min, 8% A → 15% A; 1-8 min, 15% A → 18% A; 8-10 min, 18% A → 30% A; 10-15 min, 30% A → 35% A; 15-20 min, 35% A → 85% A; 20-21 min, 85% A → 8% A; and 21-26 min, 8% A → 8% A. Chemical markers significantly affecting Moutan Cortex from various regions were screened, and their identification was based on comparison with reference materials and content determination.</p><p><strong>Results: </strong>A total of 15 chemical markers were identified, including gallic acid, oxypaeoniflorin, catechin, methyl gallate, paeonolide, apiopaeonoside, albiflorin, paeoniflorin, benzoic acid, 1,2,3,6-tetra-O-galloyl-D-glucose, 1,2,3,4,6-pentagalloylglucose, mudanpioside C, benzoyloxypaeoniflorin, benzoylpaeoniflorin, and paeonol. These markers align with component structure theory, allowing for an analysis of the structural characteristics of Moutan Cortex from different regions.</p><p><strong>Conclusions: </strong>The findings provide a valuable reference for the future quality evaluation of traditional Chinese medicine preparations, enhancing the understanding of the material basis components in Moutan Cortex from diverse sources.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipid Metabolism and Statin Therapy in Neurodegenerative Diseases: An Endocrine View.","authors":"Antonella Di Sarno, Fiammetta Romano, Rossana Arianna, Domenico Serpico, Mariarosaria Lavorgna, Silvia Savastano, Annamaria Colao, Carolina Di Somma","doi":"10.3390/metabo15040282","DOIUrl":"https://doi.org/10.3390/metabo15040282","url":null,"abstract":"<p><p><b>Background/aim</b>: A growing body of evidence suggests a link between dyslipidemias and neurodegenerative diseases, highlighting the crucial role of lipid metabolism in the health of the central nervous system. The aim of our work was to provide an update on this topic, with a focus on clinical practice from an endocrinological point of view. Endocrinologists, being experts in the management of dyslipidemias, can play a key role in the prevention and treatment of neurodegenerative conditions, through precocious and effective lipid profile optimization. <b>Methods</b>: The literature was scanned to identify clinical trials and correlation studies on the association between dyslipidemia, statin therapy, and the following neurodegenerative diseases: Alzheimer's disease (AD), Parkisons's disease (PD), Multiple sclerosis (MS), and Amyotrophic lateral sclerosis (ALS). <b>Results</b>: Impaired lipid homeostasis, such as that frequently observed in patients affected by obesity and diabetes, is related to neurodegenerative diseases, such as AD, PD, and other cognitive deficits related to aging. AD and related dementias are now a real priority health problem. In the United States, there are approximately 7 million subjects aged 65 and older living with AD and related dementias, and this number is projected to grow to 12 million in the coming decades. Lipid-lowering therapy with statins is an effective strategy in reducing serum low-density lipoprotein cholesterol to normal range concentrations and, therefore, cardiovascular disease risk; moreover, statins have been reported to have a positive effect on neurodegenerative diseases. <b>Conclusions</b>: Several pieces of research have found inconsistent information following our review. There was no association between statin use and ALS incidence. More positive evidence has emerged regarding statin use and AD/PD. However, further large-scale prospective randomized control trials are required to properly understand this issue.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143978533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Application of an In-Capillary CE-DAD Method for the Inhibitory Screening of Natural Extracts Towards Acetylcholinesterase Enzyme.","authors":"Francesca Rinaldi, Sofia Salerno, Elena Frigoli, Giulia De Soricellis, Gloria Brusotti, Stefano Negri, Matteo Radice, Francesca Merlo, Andrea Speltini, Hellas Cena, Enrica Calleri","doi":"10.3390/metabo15040283","DOIUrl":"https://doi.org/10.3390/metabo15040283","url":null,"abstract":"<p><p><b>Background:</b> The enzymatic activity of acetylcholinesterase (AChE) has been a focal point in neurodegenerative diseases research, particularly in relation to Alzheimer's disease. This is attributed to the significantly reduced levels of cholinergic neurons observed in Alzheimer's patients compared to healthy individuals. The strategy to mitigate the onset of these diseases in patients lies in the exploration of new potential AChE inhibitors with a focus also on natural extracts. A rapid and specific capillary electrophoresis method with direct ultraviolet detection (CZE-UV/Vis) was developed to screen natural extracts by assessing their potential to inhibit AChE. <b>Materials and Methods:</b> To enhance the specificity when analysing complex matrixes such as natural extracts, a sequential analysis approach based on the \"sandwich model\" was implemented using Ellman's reagent [5,5'-dithiobis-(2-nitrobenzoic acid)] (DTNB) as a colorimetric indicator. <b>Results:</b> A reference inhibitor, neostigmine, was used for system validation through IC₅₀ and K_i values determination by subsequent injections of acetylthiocholine substrate in the presence of neostigmine at increasing concentrations, and the enzyme combined with DTNB in borate-phosphate buffer (30 mM, pH 8.0). The enzymatic product was selectively detected at 412 nm. The validated system was applied to the analysis of seven natural extracts. <b>Conclusions:</b> Results demonstrated promising outcomes for identifying phytotherapeutic agents with potential applications in the prevention of neurodegenerative diseases. This method provides high selectivity and automation, offering a streamlined and effective approach for screening natural matrices containing potential AChE inhibitors.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic Effects of the Cancer Metastasis Modulator MEMO1.","authors":"Marziyeh Ghanbarian, Natalia Dolgova, Frederick S Vizeacoumar, Franco J Vizeacoumar, Deborah Michel, Anas El-Aneed, Oleg Y Dmitriev","doi":"10.3390/metabo15040277","DOIUrl":"https://doi.org/10.3390/metabo15040277","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Cancer cells often display altered energy metabolism. In particular, expression levels and activity of the tricarboxylic acid cycle (TCA cycle) enzymes may change in cancer, and dysregulation of the TCA cycle is a frequent hallmark of cancer cell metabolism. MEMO1, a modulator of cancer metastasis, has been shown to bind iron and regulate iron homeostasis in the cells. <i>MEMO1</i> knockout changed mitochondrial morphology and iron content in breast cancer cells. Our previous genome-wide analysis of <i>MEMO1</i> genetic interactions across multiple cancer cell lines revealed that gene sets involved in mitochondrial respiration and the TCA cycle are enriched among the gain-of-function interaction partners of <i>MEMO1</i>. Based on these findings, we measured the TCA cycle metabolite levels in breast cancer cells with varying levels of <i>MEMO1</i> expression. <b>Methods:</b> ShRNA knockdown assay was performed to test essentiality of key TCA cycle enzymes. TCA metabolites were quantified using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in <i>MDA-MB-231</i> (high <i>MEMO1</i>), <i>M67-2</i> (<i>MEMO1</i> knockdown), and <i>M67-9</i> (MEMO1 knockout) cells under iron-depleted, basal iron, and iron-supplemented conditions. <b>Results:</b><i>ACO2</i> and <i>OGDH</i> knockdowns inhibit cell proliferation, indicating an essential role of the TCA cycle in <i>MDA-MB-231</i> metabolism. α-Ketoglutarate and citrate levels exhibited an inverse relationship with <i>MEMO1</i> expression, increasing significantly in <i>MEMO1</i> knockout cells regardless of iron availability. In contrast, fumarate, malate, and glutamate levels were elevated in <i>MEMO1</i> knockout cells specifically under low iron conditions, suggesting an iron-dependent effect. <b>Conclusions:</b> Overall, our results indicate that MEMO1 plays a role in regulating the TCA in cancer cells in an iron-dependent manner.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Scoring Model Using Multi-Metabolites Based on Untargeted Metabolomics for Assessing Dyslipidemia in Korean Individuals with Obesity.","authors":"Su-Geun Yang, Hye Jin Yoo","doi":"10.3390/metabo15040279","DOIUrl":"https://doi.org/10.3390/metabo15040279","url":null,"abstract":"<p><strong>Background/objectives: </strong>Metabolite risk score (MRS), which considers the collective effects of metabolites closely reflecting a phenotype, is a new approach for disease assessment, moving away from focusing solely on individual biomarkers. This study aimed to investigate a metabolite panel for dyslipidemia and verify the diagnostic efficacy of MRS on dyslipidemia.</p><p><strong>Methods: </strong>Key metabolite identification and MRS establishment were conducted in the discovery set, and MRS validation was performed in the replication set, with 50 healthy individuals and 50 dyslipidemia patients in each set. The MRS was constructed using key metabolites, identified via UPLC-MS/MS analysis, employing a weighted approach based on linear regression analysis.</p><p><strong>Results: </strong>N-acetylisoputreanine-γ-lactam and eicosapentaenoic acid were identified as key metabolites for dyslipidemia and were utilized for establishing the MRS. In addition to the MRS model, a conventional dyslipidemia diagnostic model based on lipid profiles, as well as a combined model (MRS + lipid profiles), were also established. In the discovery set, the MRS model diagnosed dyslipidemia with 85.4% accuracy. When combined with lipid profiles, accuracy improved to 91.8%. In the replication set, the MRS demonstrated diagnostic power with 76.1% accuracy, while the combined model achieved 86.0% accuracy for dyslipidemia assessment.</p><p><strong>Conclusions: </strong>The MRS alone indicated sufficient assessment power in a real-world setting, despite a slight reduction in assessment ability when validated in the replication set. At this stage, therefore, the MRS serves as an auxiliary tool for disease diagnosis. This first attempt to apply MRS for dyslipidemia may offer a foundational concept for MRS in this disease.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Alpha-1-Acid Glycoprotein and Non-Alcoholic Fatty Liver Disease and Liver Fibrosis in Adult Women.","authors":"Yansong Fu, Siyi Zhang, Xin Zeng, Hong Qin","doi":"10.3390/metabo15040280","DOIUrl":"https://doi.org/10.3390/metabo15040280","url":null,"abstract":"<p><p><b>Background:</b> Alpha-1-acid glycoprotein (AGP) is a glycoprotein synthesized mainly by the liver. Nonalcoholic fatty liver disease (NAFLD) and liver fibrosis (LF) are associated with metabolic disorders. The aim of this study was to examine the potential correlation between AGP and both NAFLD and LF. <b>Methods:</b> The data were derived from the 2017-2023 National Health and Nutrition Examination Survey (NHANES). The linear association between AGP and NAFLD and LF was examined by multivariate logistic regression models. Non-linear relationships were described by fitting smoothed curves and threshold effect analysis. Subgroup analysis was also performed to assess potential regulatory factors. <b>Results:</b> The study included 2270 females. AGP was found to be significantly and positively associated with NAFLD [OR = 12.00, 95% CI (6.73, 21.39), <i>p</i> < 0.001] and LF [OR = 2.20, 95% CI (1.07, 4.50), <i>p</i> = 0.042]. Furthermore, the association between AGP and NAFLD was significantly different in the diabetic subgroup (<i>p</i> < 0.05 for interaction). Additionally, we found an inverted U-shaped relationship between AGP and controlled attenuation parameter (CAP), with an inflection point at 1.20 g/L. <b>Conclusions:</b> We found a significant positive correlation between AGP and both NAFLD and LF, and there was an inverted U-shaped relationship between AGP and CAP.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of a 6-Week Concurrent Training Program Combining Resistance and Various Modalities of Aerobic Exercise in Obese Women with Prehypertension: A Randomized Controlled Trial.","authors":"Jinhyuk Yu, Eunjoo Lee, Jae-Ho Choi, Yerin Sun, Seungyeon Woo, Sohyang Cho, Deunsol Hwang, Sung-Woo Kim, Jisu Kim, Kiwon Lim, Hun-Young Park","doi":"10.3390/metabo15040278","DOIUrl":"https://doi.org/10.3390/metabo15040278","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Our study aimed to verify the effects of 6 weeks of concurrent training composed of resistance training (RT) and different modalities of aerobic exercise (moderate-intensity continuous training (MICT) or high-intensity interval training (HIIT)) on body composition, blood pressure, vascular function, autonomic nervous system (ANS) function, blood lipid levels, cardiometabolic index (CMI), and health-related fitness in obese middle-aged women with prehypertension. <b>Methods</b>: We selected 26 middle-aged women with obesity and prehypertension and divided them equally into the RT + MICT (n = 13) and RT + HIIT (n = 13) groups. The concurrent training program consisted of warm-up, RT, aerobic exercise (MICT or HIIT), and cool-down, and was performed for 6 weeks, three times a week, 85-100 min per session. The measured dependent parameters were analyzed before and after training. <b>Results</b>: Concurrent training (RT + MICT and RT + HIIT) for 6 weeks showed significant improvements in body composition, blood pressure, vascular function, ANS function, CMI, and health-related fitness. However, the RT + HIIT group showed a relatively greater improvement in blood lipid levels compared to the RT + MICT group. <b>Conclusions</b>: Our study confirmed that both RT + MICT and RT + HIIT yielded similar positive effects on most health-related parameters in obese middle-aged women with prehypertension. Among them, RT + HIIT appeared to be relatively more effective in improving blood lipid profiles.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant Secondary Metabolites-Central Regulators Against Abiotic and Biotic Stresses.","authors":"Ameer Khan, Farah Kanwal, Sana Ullah, Muhammad Fahad, Leeza Tariq, Muhammad Tanveer Altaf, Asad Riaz, Guoping Zhang","doi":"10.3390/metabo15040276","DOIUrl":"https://doi.org/10.3390/metabo15040276","url":null,"abstract":"<p><p>As global climates shift, plants are increasingly exposed to biotic and abiotic stresses that adversely affect their growth and development, ultimately reducing agricultural productivity. To counter these stresses, plants produce secondary metabolites (SMs), which are critical biochemical and essential compounds that serve as primary defense mechanisms. These diverse compounds, such as alkaloids, flavonoids, phenolic compounds, and nitrogen/sulfur-containing compounds, act as natural protectants against herbivores, pathogens, and oxidative stress. Despite the well-documented protective roles of SMs, the precise mechanisms by which environmental factors modulate their accumulation under different stress conditions are not fully understood. This review provides comprehensive insights into the recent advances in understanding the functions of SMs in plant defense against abiotic and biotic stresses, emphasizing their regulatory networks and biosynthetic pathways. Furthermore, we explored the unique contributions of individual SM classes to stress responses while integrating the findings across the entire spectrum of SM diversity, providing a comprehensive understanding of their roles in plant resilience under multiple stress conditions. Finally, we highlight the emerging strategies for harnessing SMs to improve crop resilience through genetic engineering and present novel solutions to enhance agricultural sustainability in a changing climate.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Characterization of Serum Lipids of Dairy Cows: Effects of Negative Energy Balance on Lipid Remodelling.","authors":"Zhiqian Liu, Wenjiao Wang, Joanne E Hemsworth, Coralie M Reich, Carolyn R Bath, Monique J Berkhout, Muhammad S Tahir, Vilnis Ezernieks, Leah C Marett, Amanda J Chamberlain, Mike E Goddard, Simone J Rochfort","doi":"10.3390/metabo15040274","DOIUrl":"https://doi.org/10.3390/metabo15040274","url":null,"abstract":"<p><strong>Background: </strong>The presence and concentration of lipids in serum of dairy cows have significant implications for both animal health and productivity and are potential biomarkers for several common diseases. However, information on serum lipid composition is rather fragmented, and lipid remodelling during the transition period is only partially understood.</p><p><strong>Methods: </strong>Using a combination of reversed-phase liquid chromatography-mass spectrometry (RP-LC-MS), hydrophilic interaction-mass spectrometry (HILIC-MS), and lipid annotation software, we performed a comprehensive identification and quantification of serum of dairy cows in pasture-based Holstein-Friesian cows. The lipid remodelling induced by negative energy balance was investigated by comparing the levels of all identified lipids between the fresh lactation (5-14 days in milk, DIM) and full lactation (65-80 DIM) stages.</p><p><strong>Results: </strong>We identified 535 lipid molecular species belonging to 19 classes. The most abundant lipid class was cholesteryl ester (CE), followed by phosphatidylcholine (PC), sphingomyelin (SM), and free fatty acid (FFA), whereas the least abundant lipids included phosphatidylserine (PS), phosphatidic acid (PA), phosphatidylglycerol (PG), acylcarnitine (AcylCar), ceramide (Cer), glucosylceramide (GluCer), and lactosylceramide (LacCer).</p><p><strong>Conclusions: </strong>A remarkable increase in most lipids and a dramatic decrease in FFAs, AcylCar, and DHA-containing species were observed at the full lactation compared to fresh lactation stage. Several serum lipid biomarkers for detecting negative energy balance in cows were also identified.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted and Non-Targeted Metabolomic Evaluation of Cerebrospinal Fluid in Early Phase Schizophrenia: A Pilot Study from the Hopkins First Episode Psychosis Project.","authors":"George E Jaskiw, Mark E Obrenovich, Curtis J Donskey, Farren B S Briggs, Sun Sunnie Chung, Anastasiya I Kalinina, Austin Bolomey, Lindsay N Hayes, Kun Yang, Robert H Yolken, Akira Sawa","doi":"10.3390/metabo15040275","DOIUrl":"https://doi.org/10.3390/metabo15040275","url":null,"abstract":"<p><p>(1) Background: The lack of reliable biomarkers remains a significant barrier to improving outcomes for patients with schizophrenia. While metabolomic analyses of blood, urine, and feces have been explored, results have been inconsistent. Compared to peripheral compartments, cerebrospinal fluid (CSF) more closely reflects the chemical composition of brain extracellular fluid. Given that brain dysregulation may be more pronounced during the first episode of psychosis (FEP), we hypothesized that metabolomic analysis of CSF from FEP patients could reveal disease-associated biomarkers. (2) Methods: We recruited 15 patients within 24 months of psychosis onset (DSM-4 criteria) and 14 control participants through the Johns Hopkins Schizophrenia Center. CSF samples were analyzed using both non-targeted and targeted liquid chromatography-mass spectrometry. (3) Results: The non-targeted analysis identified lower levels of N-acetylneuraminic acid and N-acetyl-L-aspartic acid in the FEP group, while levels of uric acid were elevated. The targeted analysis focused on indolic and phenolic molecules previously linked to neuropsychiatric disorders. Notably, L-phenylalanine and 4-hydroxycinnamic acid levels were lower in the FEP group, and this difference remained significant after adjusting for age and sex. However, none of the significant differences in analyte levels between the groups survived an adjustment for multiple comparisons. (4) Conclusions: Our intriguing but preliminary associations align with results from other investigational approaches and highlight potential CSF analytes that warrant further study in larger samples.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}