Metabolites最新文献

{"title":"Therapeutic Strategies to Modulate Gut Microbial Health: Approaches for Chronic Metabolic Disorder Management.","authors":"Mariangela Rondanelli, Sara Borromeo, Alessandro Cavioni, Clara Gasparri, Ilaria Gattone, Elisa Genovese, Alessandro Lazzarotti, Leonardo Minonne, Alessia Moroni, Zaira Patelli, Claudia Razza, Claudia Sivieri, Eugenio Marzio Valentini, Gaetan Claude Barrile","doi":"10.3390/metabo15020127","DOIUrl":"10.3390/metabo15020127","url":null,"abstract":"<p><p>Numerous recent studies have suggested that the composition of the intestinal microbiota can trigger metabolic disorders, such as diabetes, prediabetes, obesity, metabolic syndrome, sarcopenia, dyslipidemia, hyperhomocysteinemia, and non-alcoholic fatty liver disease. Since then, considerable effort has been made to understand the link between the composition of intestinal microbiota and metabolic disorders, as well as the role of probiotics in the modulation of the intestinal microbiota. The aim of this review was to summarize the reviews and individual articles on the state of the art regarding ideal therapy with probiotics and prebiotics in order to obtain the reversion of dysbiosis (alteration in microbiota) to eubiosis during metabolic diseases, such as diabetes, prediabetes, obesity, hyperhomocysteinemia, dyslipidemia, sarcopenia, and non-alcoholic fatty liver diseases. This review includes 245 eligible studies. In conclusion, a condition of dysbiosis, or in general, alteration of the intestinal microbiota, could be implicated in the development of metabolic disorders through different mechanisms, mainly linked to the release of pro-inflammatory factors. Several studies have already demonstrated the potential of using probiotics and prebiotics in the treatment of this condition, detecting significant improvements in the specific symptoms of metabolic diseases. These findings reinforce the hypothesis that a condition of dysbiosis can lead to a generalized inflammatory picture with negative consequences on different organs and systems. Moreover, this review confirms that the beneficial effects of probiotics on metabolic diseases are promising, but more research is needed to determine the optimal probiotic strains, doses, and administration forms for specific metabolic conditions.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857149/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of Saliva Analytes as a Predictive Model to Detect Diseases in the Pig: A Pilot Study.","authors":"Eva Llamas-Amor, Alba Ortín-Bustillo, María José López-Martínez, Alberto Muñoz-Prieto, Edgar García Manzanilla, Julián Arense, Aida Miralles-Chorro, Pablo Fuentes, Silvia Martínez-Subiela, Antonio González-Bulnes, Elena Goyena, Andrea Martínez-Martínez, José Joaquín Cerón, Fernando Tecles","doi":"10.3390/metabo15020130","DOIUrl":"10.3390/metabo15020130","url":null,"abstract":"<p><strong>Background/objectives: </strong>Saliva is gaining importance as a diagnostic sample in pigs. The aim of this research was to evaluate a panel of salivary analytes in three porcine diseases and establish predictive models to detect them.</p><p><strong>Methods: </strong>Saliva samples were obtained from healthy pigs (<i>n</i> = 97) and pigs affected by meningitis due to <i>Streptococcus suis</i> (<i>n</i> = 118), diarrhea due to enterotoxigenic <i>Escherichia coli</i> (ETEC, <i>n</i> = 77), and porcine reproductive and respiratory syndrome (PRRS, <i>n</i> = 52). The following biomarkers were analyzed: adenosine deaminase (ADA), haptoglobin (Hp), calprotectin (Calp), aldolase, alpha-amylase (sAA), lactate dehydrogenase (LDH), total protein (TP), and advanced oxidation protein products (AOPPs). Predictive models based on binary logistic regression and decision trees combining those analytes for detecting specific diseases were constructed.</p><p><strong>Results: </strong>The results showed a different biomarker profile between the groups. <i>S. suis</i> and ETEC pigs showed higher values of ADA, Hp, Calp, aldolase, sAA, LDH, and TP than healthy pigs. Pigs with PRRS showed higher values of Hp, Calp, sAA, and LDH than healthy animals. The constructed predictive models showed overall accuracies of over 78% and 87% for differentiating ETEC and PRRS, respectively, whereas the models did not accurately predict <i>S. suis</i> infection.</p><p><strong>Conclusions: </strong>Salivary analytes show different changes in pigs depending on the disease, and the combination of these analytes can contribute to the prediction of different diseases. Further studies should be conducted in larger populations to confirm these findings and evaluate their possible practical applications.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiology-Related Variations in the Blood Hormone and Metabolome of Endangered Hog Deer (<i>Axis porcinus</i>).","authors":"Juan Wen, Bo Zhao, Yuqin Cao, Yu Qu, Liming Chang, Jie Mao, Yufei Li, Ruoyao Ni, Runliang Zhai, Jianping Jiang, Wei Zhu, Xuanzhen Liu","doi":"10.3390/metabo15020126","DOIUrl":"10.3390/metabo15020126","url":null,"abstract":"<p><p><b>Background/Objectives</b>: The hog deer (<i>Axis porcinus</i>) is an endangered species facing significant threats from habitat loss and fragmentation, with only captive populations remaining in China. Expanding breeding programs and restoring wild populations are critical strategies for the species' conservation. Achieving this requires the development of an effective health database and the identification of molecular biomarkers for their physiological traits. <b>Methods</b>: In this study, we present the largest blood metabolomics dataset to date for captive hog deer, comprising 73 healthy individuals. We conducted targeted metabolomics to quantify blood hormone levels and untargeted metabolomics to characterize blood metabolic profiles, aiming to evaluate the associations of sex, age, and weight with metabolic profiles. <b>Results</b>: Our results reveal distinct growth patterns between females and males, with males reaching their body weight plateau at a larger size. We observed significant sex differences (<i>p</i> < 0.05) in blood hormones and metabolic profiles. Females exhibited higher levels of progesterone, hydroxyprogesterone, stress hormones (e.g., cortisol), and proline, while males had higher levels of testosterone, uric acid, phenylalanine, and guanidinosuccinic acid. Notably, body weight emerged as a more important factor than gender in explaining variations in the metabolome, particularly in males. Several blood biomarkers were identified as correlating with age and body weight. Specifically, blood progesterone levels in females were linked to both age and body weight, while in males, uric acid, prolylhydroxyproline, and 3-methylhistidine were associated with these factors. The potential significance of these results for the artificial breeding and conservation of hog deer were discussed. <b>Conclusions</b>: Our study provides a metabolic reference for identifying abnormal individuals and offers potential biomarkers for determining the gender, age, and body weight of hog deer. These findings may have significant implications for the artificial breeding and conservation efforts of the species.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Dispersive Liquid-Liquid Microextraction Method for Quantification of Volatile Compounds in Wines Using Gas Chromatography-Mass Spectrometry.","authors":"Dinesha Katugampala Appuhamilage, Rebecca E Jelley, Emma Sherman, Lisa I Pilkington, Farhana R Pinu, Bruno Fedrizzi","doi":"10.3390/metabo15020129","DOIUrl":"10.3390/metabo15020129","url":null,"abstract":"<p><p><b>Background/Objectives</b>: This study reports the development of a straightforward, efficient, and cost-effective dispersive liquid-liquid microextraction (DLLME) method for the gas chromatography-mass spectrometry (GC-MS) analysis of volatile compounds present in wine. <b>Methods</b>: Four critical parameters were optimised using a D-optimal design to maximise extraction outcomes of the targeted analytes from a 10 mL sample, while minimising interference from other compounds. The analytical characteristics of the method were assessed using 36 target compounds. <b>Results</b>: The method provided satisfactory linearity (correlation coefficients > 0.990), good repeatability for both for intra- and inter-day measurements (RSD < 10.3%), and suitable recoveries of target analytes from both model (83-110%) and real matrices (80-120%). The validated method was subsequently applied to analyse the aroma profile of 30 New Zealand Pinot noir (PN) wine samples. <b>Conclusions</b>: This study contributes to the advancement of analytical techniques available to both industry and researchers to explore the complex aroma profiles of wines.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857230/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyphenols, Alkaloids, and Terpenoids Against Neurodegeneration: Evaluating the Neuroprotective Effects of Phytocompounds Through a Comprehensive Review of the Current Evidence.","authors":"Enzo Pereira de Lima, Lucas Fornari Laurindo, Vitor Cavallari Strozze Catharin, Rosa Direito, Masaru Tanaka, Iris Jasmin Santos German, Caroline Barbalho Lamas, Elen Landgraf Guiguer, Adriano Cressoni Araújo, Adriana Maria Ragassi Fiorini, Sandra Maria Barbalho","doi":"10.3390/metabo15020124","DOIUrl":"10.3390/metabo15020124","url":null,"abstract":"<p><p>Neurodegenerative diseases comprise a group of chronic, usually age-related, disorders characterized by progressive neuronal loss, deformation of neuronal structure, or loss of neuronal function, leading to a substantially reduced quality of life. They remain a significant focus of scientific and clinical interest due to their increasing medical and social importance. Most neurodegenerative diseases present intracellular protein aggregation or their extracellular deposition (plaques), such as α-synuclein in Parkinson's disease and amyloid beta (Aβ)/tau aggregates in Alzheimer's. Conventional treatments for neurodegenerative conditions incur high costs and are related to the development of several adverse effects. In addition, many patients are irresponsive to them. For these reasons, there is a growing tendency to find new therapeutic approaches to help patients. This review intends to investigate some phytocompounds' effects on neurodegenerative diseases. These conditions are generally related to increased oxidative stress and inflammation, so phytocompounds can help prevent or treat neurodegenerative diseases. To achieve our aim to provide a critical assessment of the current literature about phytochemicals targeting neurodegeneration, we reviewed reputable databases, including PubMed, EMBASE, and COCHRANE, seeking clinical trials that utilized phytochemicals against neurodegenerative conditions. A few clinical trials investigated the effects of phytocompounds in humans, and after screening, 13 clinical trials were ultimately included following PRISMA guidelines. These compounds include polyphenols (flavonoids such as luteolin and quercetin, phenolic acids such as rosmarinic acid, ferulic acid, and caffeic acid, and other polyphenols like resveratrol), alkaloids (such as berberine, huperzine A, and caffeine), and terpenoids (such as ginkgolides and limonene). The gathered evidence underscores that quercetin, caffeine, ginkgolides, and other phytochemicals are primarily anti-inflammatory, antioxidant, and neuroprotective, counteracting neuroinflammation, neuronal oxidation, and synaptic dysfunctions, which are crucial aspects of neurodegenerative disease intervention in various included conditions, such as Alzheimer's and other dementias, depression, and neuropsychiatric disorders. In summary, they show that the use of these compounds is related to significant improvements in cognition, memory, disinhibition, irritability/lability, aberrant behavior, hallucinations, and mood disorders.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Central Hypothyroidism with Low TSH Compared to Normal TSH Is Associated with More Advanced Pituitary Disease and Less Favorable Metabolic Profile.","authors":"Aleksandra E Matusiak, Jan Stępniak, Krzysztof C Lewandowski, Andrzej Lewiński, Małgorzata Karbownik-Lewińska","doi":"10.3390/metabo15020125","DOIUrl":"10.3390/metabo15020125","url":null,"abstract":"<p><p><b>Background:</b> Central hypothyroidism is characterized by either decreased TSH or, more commonly, normal TSH. This study aims to check whether this biochemical difference related to the severity of the pituitary disease, metabolic processes and general well-being. <b>Methods:</b> A retrospective analysis was performed on 108 inpatients with hypopituitarism, aged 18-80, hospitalized (1 January 2020, through 31 December 2022) in the Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, Poland. Hypopituitary patients with central hypothyroidism (<i>n</i> = 90) were divided into two subgroups: patients with TSH below normal ranges (low TSH; <i>n</i> = 52) and patients with TSH in reference ranges (normal TSH; <i>n</i> = 38). <b>Results:</b> Among patients with central hypothyroidism, surgical treatment due to pituitary disease was performed more commonly in those with low TSH than in those with normal TSH (65 vs. 42%, <i>p</i> = 0.010). Expectedly, five pituitary deficiencies were diagnosed more commonly in patients with low TSH than in those with normal TSH (46 vs. 13%, <i>p</i> = 0.001). In a regression analysis, the ACTH concentration was the only independent determinant negatively associated with low TSH (also after limiting the analysis to non-treated patients). Regarding lipid profile, decreased HDL cholesterol occurred more commonly in patients with low TSH vs. normal TSH (44% vs. 23%; <i>p</i> = 0.033), which was also observed after the limitation to non-treated patients (47% vs. 21%; <i>p</i> = 0.013). <b>Conclusions:</b> Low TSH in patients with central hypothyroidism is associated with more advanced pituitary disease and less favorable metabolic profile.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857551/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brain Glycogen-Its Metabolic Role in Neuronal Health and Neurological Disorders-An Extensive Narrative Review.","authors":"Ana Isabel Beltran-Velasco","doi":"10.3390/metabo15020128","DOIUrl":"10.3390/metabo15020128","url":null,"abstract":"<p><p><b>Background:</b> Brain glycogen is imperative for neuronal health, as it supports energy demands and metabolic processes. This review examines the pathways involved in glycogen storage and utilization in the central nervous system, emphasizing their role in both physiology and pathology. It explores how alterations in glycogen metabolism contribute to neurological disorders, including neurodegenerative diseases, epilepsy, and metabolic conditions while highlighting the bidirectional interaction between neurons and glia in maintaining brain homeostasis. <b>Methods:</b> A comprehensive search of articles published between 2015 and 2025 was conducted using the following databases: ScienceDirect, Scopus, Wiley, Web of Science, Medline, and PubMed. The selection of relevant studies was based on their focus on brain glycogen metabolism and its role in neurological conditions, with studies that did not meet the inclusion criteria being excluded. <b>Results:</b> The metabolic processes of brain glycogen are subject to rigorous regulation by astrocyte-neuron interactions, thereby ensuring metabolic homeostasis and energy availability. The dysregulation of glycogen storage and mobilization has been implicated in the development of synaptic dysfunction, excitotoxicity, and neurodegeneration in a variety of disorders. For instance, aberrant glycogen accumulation in diseases such as Lafora disease has been associated with severe neurodegeneration, while impaired glycogen mobilization has been shown to exacerbate energy deficits in Alzheimer's and epilepsy. <b>Conclusions:</b> Targeting brain glycogen metabolism represents a promising approach for therapeutic intervention in neurological disorders. However, the translation of these strategies to human models remains challenging, particularly with regard to the long-term safety and specificity of glycogen-targeted therapies.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857135/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics Profiling and Advanced Methodologies for Wheat Stress Research.","authors":"Zhen Liu, Jiahui You, Peiying Zhao, Xianlin Wang, Shufang Sun, Xizhen Wang, Shubo Gu, Qian Xu","doi":"10.3390/metabo15020123","DOIUrl":"10.3390/metabo15020123","url":null,"abstract":"<p><p>Metabolomics is an omics technology that studies the types, quantities, and changes of endogenous metabolic substances in organisms affected by abiotic and biotic factors.</p><p><strong>Background/objectives: </strong>Based on metabolomics, small molecule metabolites in biological organisms can be qualitatively and quantitatively analysed. This method analysis directly correlates with biological phenotypes, facilitating the interpretation of life conditions. Wheat (<i>Triticum aestivum</i> L.) is one of the major food crops in the world, and its quality and yield play important roles in safeguarding food security.</p><p><strong>Methods: </strong>This review elaborated on the significance of metabolomics research techniques and methods in enhancing wheat resilience against biotic and abiotic stresses.</p><p><strong>Results: </strong>Metabolomics plays an important role in identifying the metabolites in wheat that respond to diverse stresses. The integrated examination of metabolomics with other omics disciplines provides new insights and approaches for exploring resistance genes, understanding the genetic basis of wheat metabolism, and revealing the mechanisms involved in stress responses.</p><p><strong>Conclusions: </strong>Emerging metabolomics research techniques to propose innovative avenues of research is important to enhance wheat resistance.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857233/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics Analysis Reveals Characteristic Functional Components in Pigeon Eggs.","authors":"Rui Zhang, Lingling Chang, Xinyue Shen, Qingping Tang, Chunyu Mu, Shengyong Fu, Zhu Bu","doi":"10.3390/metabo15020122","DOIUrl":"10.3390/metabo15020122","url":null,"abstract":"<p><p>We aimed to identify the characteristic functional components of pigeon eggs and the differences among pigeon, chicken, and quail eggs. We analyzed the metabolite profiles of three kinds of eggs using an untargeted metabolomics-based approach to better understand the differences in metabolites among pigeon, chicken, and quail eggs. Then, we quantitatively validated the differences in abundance of partial metabolites through a targeted metabolomics-based approach. A total of 692 metabolites were identified in the three types of eggs. A total of 263 significantly differentially abundant metabolites were found between pigeon eggs and chicken eggs, and 263 significantly differentially abundant metabolites were found between pigeon eggs and quail eggs. The metabolites that were significantly more abundant in pigeon eggs than in other eggs were mainly lipids, lipid-like molecules, nucleosides, nucleotides, and their analogues. We identified the eight metabolites that were significantly greater in abundance in pigeon eggs than in chicken eggs and quail eggs and quantitatively validated the differences in abundance of these metabolites. Our study demonstrates that there are more functional components in pigeon eggs than chicken eggs and quail eggs, especially for the prevention and treatment of various disordered glucose and lipid metabolism-related diseases. The discovery of these differentially abundant metabolites paves the way for further research on the unique nutritional functions of pigeon eggs and the further utilization of pigeon egg products.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifluid Metabolomics Identifies Novel Biomarkers for Irritable Bowel Syndrome.","authors":"Daniel Kirk, Panayiotis Louca, Ilias Attaye, Xinyuan Zhang, Kari E Wong, Gregory A Michelotti, Mario Falchi, Ana M Valdes, Frances M K Williams, Cristina Menni","doi":"10.3390/metabo15020121","DOIUrl":"10.3390/metabo15020121","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Irritable bowel syndrome (IBS) is a complex disorder affecting 10% of the global population, but the underlying mechanisms remain poorly understood. By integrating multifluid metabolomics, we aimed to identify metabolite markers of IBS in a large population-based cohort. <b>Methods</b>: We included individuals from TwinsUK with and without IBS, ascertained using the Rome III criteria, and analysed serum (232 cases, 1707 controls), urine (185 cases, 1341 controls), and stool (186 cases, 1284 controls) metabolites (Metabolon Inc.). <b>Results</b>: After adjusting for covariates, and multiple testing, 44 unique metabolites (25 novel) were associated with IBS, including lipids, amino acids, and xenobiotics. Androsterone sulphate, a sulfated steroid hormone precursor, was associated with lower odds of IBS in both urine (0.69 [95% confidence interval = 0.56-0.85], <i>p</i> = 2.34 × 10^-4) and serum (0.75 [0.63-0.90], <i>p</i> = 1.54 × 10^-3. Moreover, suberate (C8-DC) was associated with higher odds of IBS in serum (1.36 [1.15-1.61]; <i>p</i> = 1.84 × 10^-4) and lower odds of IBS in stool (0.76 [0.63-0.91]; <i>p</i> = 2.30 × 10^-3). On the contrary, 32 metabolites appeared to be fluid-specific, including indole, 13-HODE + 9-HODE, pterin, bilirubin (E,Z or Z,Z), and urolithin. The remaining 10 metabolites were associated with IBS in one fluid with suggestive evidence (<i>p</i> < 0.05) in another fluid. Finally, we identified androgenic signalling, dicarboxylates, haemoglobin, and porphyrin metabolism to be significantly over-represented in individuals with IBS compared to controls. <b>Conclusions</b>: Our results highlight the utility of a multi-fluid approach in IBS research, revealing distinct metabolic signatures across biofluids.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 2","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}