Metabolites最新文献

{"title":"In Vitro Antioxidant, Antithrombotic and Anti-Inflammatory Activities of Bioactive Metabolites Extracted from Kiwi and Its By-Products.","authors":"Anastasia Maria Moysidou, Konstantina Cheimpeloglou, Spyridoula Ioanna Koutra, Vasileios Manousakis, Anna Ofrydopoulou, Katie Shiels, Sushanta Kumar Saha, Alexandros Tsoupras","doi":"10.3390/metabo15060400","DOIUrl":"10.3390/metabo15060400","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Growing interest in natural, health-promoting ingredients for functional foods, nutraceuticals, and cosmetics has increased the demand for bioactive compounds from kiwi (Actinidia deliciosa). This study aimed to assess the antioxidant, anti-inflammatory, and antithrombotic properties of amphiphilic bioactives extracted from kiwi fruit and its by-products, including peel, seeds, and pulp. <b>Methods:</b> Bioactive compounds were extracted and analyzed using liquid chromatography-mass spectrometry (LC-MS) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. Antioxidant activity was evaluated using DPPH and ABTS radical scavenging assays. Anti-inflammatory and antithrombotic effects were assessed through inhibition of platelet aggregation induced by platelet-activating factor (PAF) and adenosine diphosphate (ADP) in human platelets. <b>Results:</b> All extracts showed significant antioxidant activity. FTIR and LC-MS analyses confirmed the presence of phenolics, flavonoids, carotenoids, and polar lipids. Kiwi peel extract exhibited the strongest inhibition of PAF- and ADP-induced platelet aggregation, attributed to its higher content of phenolics and unsaturated polar lipids. LC-MS data indicated a favorable fatty acid profile with high omega-9 levels and a low omega-6/omega-3 ratio. Polar lipid structural analysis revealed a predominance of phospholipids with unsaturated fatty acids at the sn-2 position. <b>Conclusions:</b> Kiwi by-products are valuable sources of health-promoting bioactives with antioxidant and anti-inflammatory potential. These findings support their incorporation into nutraceutical, nutricosmetic, and cosmeceutical products and lay the groundwork for further studies on safety, efficacy, and practical application.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194973/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Systems Hypothesis of Lipopolysaccharide-Induced Vitamin Transport Suppression and Metabolic Reprogramming in Autism Spectrum Disorders: An Open Call for Validation and Therapeutic Translation.","authors":"Albion Dervishi","doi":"10.3390/metabo15060399","DOIUrl":"10.3390/metabo15060399","url":null,"abstract":"<p><p><b>Background:</b> Autism spectrum disorder (ASD) is increasingly linked to systemic metabolic dysfunction, potentially influenced by gut-brain axis dysregulation, but the underlying mechanisms remain unclear. <b>Methods:</b> We developed Personalized Metabolic Margin Mapping (PM³), a computational systems biology framework, to analyze RNA-seq data from 12 ASD and 12 control postmortem brain samples. The model focused on 158 curated metabolic genes selected for their roles in redox balance, mitochondrial function, neurodevelopment, and gut-brain interactions. <b>Results:</b> Using unsupervised machine learning (Isolation Forest) to detect outlier expression patterns, Euclidean distance, and percent expression difference metrics, PM³ revealed a consistent downregulation of glycolysis (e.g., -5.4% in PFKM) and mitochondrial enzymes (e.g., -12% in SUCLA2). By incorporating cofactor dependency and subcellular localization, PM³ identified a coordinated suppression of multivitamin transporters (e.g., -4.5% in SLC5A6, -3.5% in SLC19A2), potentially limiting cofactor availability and compounding energy deficits in ASD brains. <b>Conclusions:</b> These findings suggest a convergent metabolic dysregulation signature in ASD; wherein the subtle suppression of cofactor-dependent pathways may impair energy metabolism and neurodevelopment. We propose that chronic microbial lipopolysaccharide (LPS) exposure in ASD suppresses vitamin transporter function, initiating mitochondrial dysfunction and transcriptomic reprogramming. Validation in LPS-exposed systems using integrated transcriptomic-metabolomic analysis is warranted.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195403/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bile Acids and Type 2 Diabetes: Roles in Glucose Homeostasis and Therapeutic Opportunities.","authors":"Yiting Lin, Chunyan Hu, Shuangyuan Wang, Hong Lin","doi":"10.3390/metabo15060401","DOIUrl":"10.3390/metabo15060401","url":null,"abstract":"<p><strong>Background: </strong>Type 2 diabetes mellitus (T2DM), characterized by impaired glucose homeostasis, represents a significant threat to public health. Bile acids (BAs), as key metabolic regulators, play an essential role in glucose metabolism. Recent advances in high-resolution metabolomics have revealed that various BA species are closely linked to T2DM pathogenesis and play a critical role in maintaining glucose homeostasis. Understanding the underlying mechanisms by which BAs modulate glucose metabolism provides valuable insights for the prevention and treatment of T2DM.</p><p><strong>Methods/results: </strong>This review describes the roles of diverse BA species in regulating glucose metabolism and comprehensively summarizes the relationship of unconjugated and conjugated BAs with T2DM in population studies. Furthermore, we discuss BA-targeted therapeutic approaches for T2DM, highlighting the urgent need for developing tissue-restricted modulators of BA receptors and advancing the clinical translation of novel beneficial BAs.</p><p><strong>Conclusion: </strong>Deeply understanding the role of BAs played in the pathogenesis and progression of T2DM will facilitate the development of potential therapeutic agents.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcomes of Different Lifestyle Approaches in a Multicentre, Open-Label, Parallel-Group, Randomised Controlled Trial of the Effectiveness of Integrating a Pragmatic Pathway for Prescribing Liraglutide 3.0 mg in Weight Management Services (STRIVE Study).","authors":"Werd Al-Najim, Babak Dehestani, Ahmed W Al-Humadi, Danielle H Bodicoat, Dimitris Papamargaritis, Michael Lean, Barbara McGowan, David R Webb, John Ph Wilding, Melanie J Davies, Carel W le Roux","doi":"10.3390/metabo15060398","DOIUrl":"10.3390/metabo15060398","url":null,"abstract":"<p><p><b>Background/Objectives:</b> The STRIVE study was a multicentre, open-label, real-world clinical trial evaluating the effectiveness of a targeted prescribing pathway for liraglutide 3.0 mg as an adjunct to standard care versus standard care alone in people with obesity attending Specialist Weight Management Services (SWMS) in the UK and Ireland. This post hoc analysis focuses on the standard care arm to explore differences in outcomes between sites, particularly the potential impact of offering meal replacements as part of usual care. <b>Methods:</b> Participants included individuals with a BMI ≥ 35 kg/m² and at least one obesity-related complication who received standard care at five SWMS sites. All sites provided specialist nutrition and exercise counselling; however, only the Dublin site (n = 40) included meal replacements as part of routine care. Baseline characteristics and weight change data were compared between the Dublin and UK cohorts (n = 92) at 52 and 104 weeks. Statistical comparisons were made using appropriate parametric and non-parametric tests. <b>Results:</b> At baseline, the Dublin cohort was significantly older (<i>p</i> < 0.01), had a higher prevalence of hypertension (<i>p</i> < 0.05), and a lower reported incidence of depression/anxiety (<i>p</i> < 0.05) than the UK cohort. At week 52, the Dublin group achieved greater mean weight loss (-6.1%, SD ± 5.7%) compared to the UK cohort (-1.3%, SD ± 6.7%, n = 27, <i>p</i> < 0.01). By week 104, Dublin participants maintained a mean weight loss of -4.4% (SD ± 5.7%) while UK participants had a mean weight gain of 0.37% (SD ± 7.6%) (<i>p</i> < 0.05). <b>Conclusions:</b> The integration of meal replacements as part of usual care may have contributed to the greater and sustained weight loss observed in the Dublin cohort compared to other SWMS in the UK.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12194874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolite Profile and Metabolic Network Analysis of Walnuts (<i>Juglans regia</i> L.) in Response to Chilling Stress.","authors":"Kai Liu, Yang Li, Yaxin Sang, Yaru Zhang, Xiuhong An, Hongxia Wang, Ruifen Zhang","doi":"10.3390/metabo15060394","DOIUrl":"10.3390/metabo15060394","url":null,"abstract":"<p><strong>Background: </strong>Walnut (<i>Juglans regia</i> L.) is a species of considerable ecological, social, and economic importance. However, comprehensive metabolomic investigations into walnut cultivars under chilling stress remain scarce.</p><p><strong>Methods: </strong>In this study, we utilized LC-MS/MS-based non-targeted metabolomics to analyze differential metabolites in two walnut cultivars exposed to chilling stress at 0.5 °C for 0 and 48 h.</p><p><strong>Results: </strong>A total of 1504 metabolites were identified, including 871 in positive ion mode and 633 in negative ion mode. Specifically, 160 and 287 differential metabolites were detected in 'Qingxiang' and 'Liaoning No.8', respectively, under positive ion mode. In negative ion mode, 83 and 206 differential metabolites were identified in 'Qingxiang' and 'Liaoning No.8', respectively. These metabolites were primarily associated with α-linolenic acid metabolism, phenylpropanoid biosynthesis, flavonoid biosynthesis, and phenylalanine metabolism, and multiple candidate genes were obtained that exhibit significant correlations with metabolites, suggesting their critical roles in the walnut's response to chilling stress.</p><p><strong>Conclusions: </strong>This study proposes a metabolic network for walnut leaves under chilling stress, enriching our understanding of the metabolic adaptation mechanisms of walnuts to low-temperature conditions. It lays a foundation for investigating the regulatory mechanisms of metabolite synthesis under cold stress and provides important theoretical insights for breeding cold-resistant walnut cultivars.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195032/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial Translation Inhibition Uncovers a Critical Metabolic-Epigenetic Interface in Renal Cell Carcinoma.","authors":"Kazumi Eckenstein, Beyza Cengiz, Matthew E K Chang, Jessie May Cartier, Mark R Flory, George V Thomas","doi":"10.3390/metabo15060393","DOIUrl":"10.3390/metabo15060393","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Renal cell carcinoma (RCC) exhibits distinctive metabolic vulnerabilities that may be therapeutically targeted. This study investigates how tigecycline, an FDA-approved antibiotic that inhibits mitochondrial translation, affects RCC cells and explores potential combinatorial approaches to enhance its efficacy. <b>Methods:</b> We employed comprehensive metabolomic profiling, subcellular proteomics, and functional assays to characterize the effects of tigecycline on RCC cell lines, patient-derived organoids, and xenograft models. The synergistic potential of tigecycline with the histone deacetylase inhibitor entinostat was evaluated using combination index analysis. <b>Results:</b> Tigecycline selectively inhibited mitochondrial translation in RCC cells, reducing mitochondrially-encoded proteins while sparing nuclear-encoded components, profoundly disrupting mitochondrial bioenergetics and reducing tumor growth in xenograft models. Subcellular proteomic analyses revealed that tigecycline treatment triggered a significant accumulation of multiple histone variants concurrent with cell cycle arrest. Based on this discovery, combined treatment with tigecycline and entinostat demonstrated remarkable synergism across RCC cell lines and patient-derived. <b>Conclusions:</b> Our findings identify a promising therapeutic opportunity by targeting the crosstalk between mitochondrial function and epigenetic homeostasis in RCC, with the potential for rapid clinical translation given the established pharmacological profiles of both agents.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut Microbiota Dysbiosis and Its Impact on Type 2 Diabetes: From Pathogenesis to Therapeutic Strategies.","authors":"Yonghua Yu, Yilan Ding, Shuangyuan Wang, Lei Jiang","doi":"10.3390/metabo15060397","DOIUrl":"10.3390/metabo15060397","url":null,"abstract":"<p><p>Type 2 diabetes mellitus (T2DM) is a common metabolic disorder characterized by insulin resistance and pancreatic β-cell dysfunction. Emerging evidence indicates that gut microbiota dysbiosis may contribute to the development of T2DM. Individuals with T2DM exhibit notable changes in gut microbiota composition, including shifts in the balance between Firmicutes and Bacteroidetes, a reduction in butyrate-producing bacteria, and an increase in opportunistic pathogens. Gut microbiota-derived metabolites-such as short-chain fatty acids, bile acids, and amino acids-have been implicated in the pathogenesis of T2DM, highlighting the critical role of host-microbe interactions. In this overview, we discuss the gut microbiota dysbiosis associated with T2DM and explore the molecular links between microbiota-derived metabolites and the pathogenesis of diseases. Additionally, we explore potential therapeutic strategies, including probiotics and dietary interventions, to modulate the gut microbiota and its metabolites, providing insights for future clinical research and the development of novel treatments for T2DM.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blood Metabolic Biomarkers of Diabetes Mellitus Type 2 in Aged Adults Determined by a UPLC-MS Metabolomic Approach.","authors":"Alba Simón, Daniel Bordonaba-Bosque, Olimpio Montero, Javier Solano-Castán, Irma Caro","doi":"10.3390/metabo15060395","DOIUrl":"10.3390/metabo15060395","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Type 2 diabetes mellitus (T2DM) is a metabolic disease whose importance rises with aging, though it is also looming large in younger populations due to increasing obesity. Its effects may damage renal and heart functioning. Plasma biomarkers of T2DM have been shown through metabolomic studies under different conditions, mainly obesity, but untargeted metabolomic studies on T2DM are lacking for elderly people. <b>Methods:</b> A UPLC-MS-based metabolomic approach was conducted to ascertain potential plasma biomarkers in a cohort older than 65 years. <b>Results:</b> The dipeptide Gly-His, along with diverse lysophosphatidylcholines (LPCs), mainly LPC(14:0) and LPC(20:4), and three gangliosides were found to have different plasma content in T2DM subjects compared to control (non-diabetic) subjects (NT2DM). LPC(20:4) exhibited a gender dependence, with statistically significant differences only in females. Gly-His correlated with MEDAS-14, whereas LPC(14:0) correlated with sugar-rich food consumption. <b>Conclusions:</b> As previously demonstrated for other conditions, mainly obesity, altered lipid metabolism was shown in this study to be a hallmark of T2DM in elderly people also.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144484998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining Metabolomics and Proteomics to Reveal Key Serum Compounds Related to Canine Intervertebral Disc Herniation.","authors":"Anita Horvatić, Josipa Kuleš, Andrea Gelemanović, Ozren Smolec, Boris Pirkić, Marko Pećin, Ivana Rubić, Vladimir Mrljak, Marko Samardžija, Marija Lipar","doi":"10.3390/metabo15060396","DOIUrl":"10.3390/metabo15060396","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Canine intervertebral disc herniation (IVDH) is an important musculoskeletal pathology. Unlike in humans, IVDH mechanisms in dogs are underinvestigated from a system-level integrative omics point of view. The aim of this study was to identify key serum molecular players in canine IVDH. <b>Methods</b>: An integrative multi-omics approach combining high-resolution LC-MS-based untargeted metabolomics and tandem mass tag (TMT)-based proteomics was applied. Additionally, serum zinc concentration was determined by spectrophotometry. <b>Results</b>: Nineteen serum metabolites were differentially abundant in IVDH dogs. Metabolite analysis highlighted dysregulation in lipoic acid and branched-chain amino acid (BCAA) metabolism, with elevated levels of valine, leucine, and isoleucine in IVDH. These findings suggest disrupted energy, nitrogen, and neurotransmitter metabolism, potentially contributing to the IVDH pathophysiology. Additionally, lower serum uridine, possibly influenced by BCAA accumulation, was observed, indicating altered neuroinflammatory responses. ELISA validation confirmed elevated serum levels of zinc-α2-glycoprotein (ZAG), alpha-1-microglobulin/bikunin precursor (AMBP), and vitronectin (VTN) in IVDH, supporting immune modulation and neuroprotective mechanisms. Serum prekallikrein (KLKB1) and Protein C inhibitor (SERPINA5), involved in fibrin cloth formation, were found to be lowered in IVDH patients. Pathway enrichment revealed disturbances in aromatic amino acid biosynthesis, with elevated phenylalanine, tyrosine, and tryptophan influencing neurotransmission and inflammation. In addition, elevated serum Zn concentration emphasized its antioxidant importance in immune response, wound healing, and neuropathic pain signaling. <b>Conclusions</b>: Integration with our prior CSF multi-omics data reinforced the relevance of identified molecules in IVDH-associated neurodegeneration, inflammation, and repair processes. This study offers insight into potential diagnostic biomarkers and therapeutic targets for canine IVDH through serum-based molecular profiling.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential Expression of Lipid Metabolism Genes, CROT and ABCG1, in Obese Patients with Comorbid Depressive Disorder and Risk of MASLD.","authors":"Joanna Michalina Jurek, Elena Cristina Rusu, Javier Camaron, Helena Clavero-Mestres, Carmen Aguilar, David Riesco, Belen Xifré, Javier U Chicote, Salomé Martinez, Marga Vives, Fàtima Sabench, Teresa Auguet","doi":"10.3390/metabo15060392","DOIUrl":"10.3390/metabo15060392","url":null,"abstract":"<p><strong>Background: </strong>There is accumulating evidence supporting a bidirectional relationship between metabolic dysfunction-associated steatotic liver disease (MASLD) and depressive disorder (DD), with possible genetic factors related to hepatic lipid metabolism. Our aim was to analyse the prevalence of DD in patients with obesity at risk of MASLD, and to evaluate the hepatic expression of genes involved in lipid metabolism, in patients with DD.</p><p><strong>Methods: </strong>In 152 patients with morbid obesity who underwent bariatric surgery, medical data, blood and liver samples were collected. Liver biopsies were scored for MASLD staging were used for gene expression analysis.</p><p><strong>Results: </strong>The DD prevalence in this cohort was 29.6%, and patients with DD had a significantly higher hepatic expression of the <i>CROT</i> and <i>ABCG1</i> genes. Moreover, patients in the MASLD group showed significantly higher relative hepatic expression of <i>SREBP1</i> and <i>ABCG1</i> genes compared to the normal liver group. Some anthropometric and clinical measures (BMI and DBP) were positively correlated with the expression of <i>SREBP2, ABCG1</i> and <i>CROT</i> genes, while the expression of <i>CPT1α</i> was negatively correlated with age, SBP and DBP. There was a positive relationship between GGT and ALP levels and the relative expression of <i>ABCG1</i> and <i>ACC1</i> genes.</p><p><strong>Conclusions: </strong>In this study, individuals with morbid obesity demonstrated an elevated prevalence of DD. Moreover, hepatic genetic dysregulation of lipid metabolism may influence the interplay between MASLD and DD in patients with morbid obesity.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 6","pages":""},"PeriodicalIF":3.4,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12195504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144485012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}