UPLC-MS代谢组学方法测定老年人2型糖尿病血液代谢生物标志物

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2025-06-12 DOI:10.3390/metabo15060395

Alba Simón, Daniel Bordonaba-Bosque, Olimpio Montero, Javier Solano-Castán, Irma Caro

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引用次数: 0

摘要

背景/目的：2型糖尿病（T2DM）是一种代谢性疾病，其重要性随着年龄的增长而上升，尽管由于肥胖的增加，它在年轻人群中也越来越突出。其影响可能损害肾脏和心脏功能。T2DM的血浆生物标志物已经通过代谢组学研究在不同条件下，主要是肥胖，但缺乏针对老年人T2DM的非靶向代谢组学研究。方法：采用基于uplc - ms的代谢组学方法确定65岁以上人群的潜在血浆生物标志物。结果：T2DM患者血浆中二肽Gly-His、多种溶血磷脂酰胆碱（LPCs），主要是LPC（14:0）和LPC(20:4)，以及三种神经节苷类物质的含量与对照组（非糖尿病）（NT2DM）存在差异。LPC（20:4）表现出性别依赖性，仅在女性中差异有统计学意义。Gly-His与MEDAS-14相关，而LPC（14:0）与富含糖的食物摄入相关。结论：正如先前在其他情况下（主要是肥胖）所证明的那样，本研究显示脂质代谢改变也是老年人2型糖尿病的一个标志。

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Blood Metabolic Biomarkers of Diabetes Mellitus Type 2 in Aged Adults Determined by a UPLC-MS Metabolomic Approach.

Background/Objectives: Type 2 diabetes mellitus (T2DM) is a metabolic disease whose importance rises with aging, though it is also looming large in younger populations due to increasing obesity. Its effects may damage renal and heart functioning. Plasma biomarkers of T2DM have been shown through metabolomic studies under different conditions, mainly obesity, but untargeted metabolomic studies on T2DM are lacking for elderly people. Methods: A UPLC-MS-based metabolomic approach was conducted to ascertain potential plasma biomarkers in a cohort older than 65 years. Results: The dipeptide Gly-His, along with diverse lysophosphatidylcholines (LPCs), mainly LPC(14:0) and LPC(20:4), and three gangliosides were found to have different plasma content in T2DM subjects compared to control (non-diabetic) subjects (NT2DM). LPC(20:4) exhibited a gender dependence, with statistically significant differences only in females. Gly-His correlated with MEDAS-14, whereas LPC(14:0) correlated with sugar-rich food consumption. Conclusions: As previously demonstrated for other conditions, mainly obesity, altered lipid metabolism was shown in this study to be a hallmark of T2DM in elderly people also.

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.