Prediagnostic Plasma Metabolomic Profiles Using NMR for Exfoliation Glaucoma Among US Health Professionals.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2025-07-09 DOI:10.3390/metabo15070469

Akiko Hanyuda, Oana A Zeleznik, Yoshihiko Raita, Danielle E Haslam, Qi Sun, Kazuno Negishi, Louis R Pasquale, Jessica Lasky-Su, Janey L Wiggs, Jae H Kang

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引用次数: 0

Abstract

Background: Exfoliation glaucoma (XFG) represents a form of deleterious ocular aging of unclear etiology. We evaluated prediagnostic nuclear magnetic resonance (NMR)-based metabolites in relation to XFG risk, expanding on our prior findings of XFG-related metabotypes using liquid chromatography-mass spectrometry (LC-MS).

Methods: We identified 217 XFG cases and 217 matched controls nested within three prospective health professional cohorts with plasma collected a mean 11.8 years before case identification. Plasma metabolites were analyzed using the targeted NMR Nightingale platform. Conditional logistic models and Metabolite Set Enrichment Analysis were performed. Multiple comparison issues were addressed using the number of effective tests (NEF) and false discovery rate (FDR).

Results: Among 235 profiled metabolites, higher glucose was significantly associated with a lower risk of XFG (odds ratio (95%CI) = 0.42 (0.26, 0.7); NEF = 0.03). Among metabolite classes, lipoprotein subclasses and branched-chain amino acids were inversely associated, while relative lipoprotein lipid concentrations were adversely associated (FDR < 0.05).

Conclusion: NMR profiling revealed that glucose, branched-chain amino acids, lipoprotein subclasses, and relative lipoprotein lipid concentrations may play important roles in XFG etiology.

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在美国卫生专业人员中，使用核磁共振对脱落性青光眼进行诊断前血浆代谢组学分析。

背景：剥脱性青光眼（XFG）是一种病因不明的有害眼部老化。我们评估了基于核磁共振（NMR）的诊断前代谢物与XFG风险的关系，扩展了我们之前使用液相色谱-质谱（LC-MS）发现的XFG相关代谢物类型。方法：我们在三个前瞻性卫生专业人员队列中确定了217例XFG病例和217例匹配的对照，在病例确定之前平均11.8年收集血浆。使用靶向NMR Nightingale平台分析血浆代谢物。进行条件logistic模型和代谢物集富集分析。使用有效测试数（NEF）和错误发现率（FDR）解决了多个比较问题。结果：在235种代谢物中，较高的葡萄糖与较低的XFG风险显著相关(优势比(95%CI) = 0.42 (0.26, 0.7)；Nef = 0.03)。在代谢物类别中，脂蛋白亚类与支链氨基酸呈负相关，而脂蛋白脂质相对浓度呈负相关（FDR < 0.05）。结论：核磁共振图谱显示葡萄糖、支链氨基酸、脂蛋白亚类和相对脂蛋白脂质浓度可能在XFG病因学中起重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.