Marine Drugs最新文献_第8页

Exploring the Diversity and Specificity of Secondary Biosynthetic Potential in Rhodococcus 探索 Rhodococcus 次生生物合成潜能的多样性和特异性

IF 5.4 2区医学

Marine Drugs Pub Date : 2024-09-06 DOI: 10.3390/md22090409

Gang-Ao Hu, Yue Song, Shi-Yi Liu, Wen-Chao Yu, Yan-Lei Yu, Jian-Wei Chen, Hong Wang, Bin Wei

{"title":"Exploring the Diversity and Specificity of Secondary Biosynthetic Potential in Rhodococcus","authors":"Gang-Ao Hu, Yue Song, Shi-Yi Liu, Wen-Chao Yu, Yan-Lei Yu, Jian-Wei Chen, Hong Wang, Bin Wei","doi":"10.3390/md22090409","DOIUrl":"https://doi.org/10.3390/md22090409","url":null,"abstract":"The actinomycete genus Rhodococcus is known for its diverse biosynthetic enzymes, with potential in pollutant degradation, chemical biocatalysis, and natural product exploration. Comparative genomics have analyzed the distribution patterns of non-ribosomal peptide synthetases (NRPSs) in Rhodococcus. The diversity and specificity of its secondary metabolism offer valuable insights for exploring natural products, yet remain understudied. In the present study, we analyzed the distribution patterns of biosynthetic gene clusters (BGCs) in the most comprehensive Rhodococcus genome data to date. The results show that 86.5% of the gene cluster families (GCFs) are only distributed in a specific phylogenomic-clade of Rhodococcus, with the most predominant types of gene clusters being NRPS and ribosomally synthesized and post-translationally modified peptides (RiPPs). In-depth mining of RiPP gene clusters revealed that Rhodococcus encodes many clade-specific novel RiPPs, with thirteen core peptides showing antibacterial potential. High-throughput elicitor screening (HiTES) and non-targeted metabolomics revealed that a marine-derived Rhodococcus strain produces a large number of new aurachin-like compounds when exposed to specific elicitors. The present study highlights the diversity and specificity of secondary biosynthetic potential in Rhodococcus, and provides valuable information for the targeted exploration of novel natural products from Rhodococcus, especially for phylogenomic-clade-specific metabolites.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"50 1","pages":""},"PeriodicalIF":5.4,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142211311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Cytochalasins and Polyketides from a Mangrove Endophytic Fungus Xylaria arbuscula QYF 红树林内生真菌 Xylaria arbuscula QYF 的细胞色素和多酮类化合物

IF 5.4 2区医学

Marine Drugs Pub Date : 2024-09-05 DOI: 10.3390/md22090407

Qi Tan, Xinyu Ye, Siqi Fu, Yihao Yin, Yufeng Liu, Jianying Wu, Fei Cao, Bo Wang, Tingshun Zhu, Wencong Yang, Zhigang She

引用次数: 0

Sphaerococcenol A Derivatives: Design, Synthesis, and Cytotoxicity Sphaerococcenol A 衍生物：设计、合成和细胞毒性

IF 5.4 2区医学

Marine Drugs Pub Date : 2024-09-05 DOI: 10.3390/md22090408

Dídia Sousa, Milene A. G. Fortunato, Joana Silva, Mónica Pingo, Alice Martins, Carlos A. M. Afonso, Rui Pedrosa, Filipa Siopa, Celso Alves

{"title":"Sphaerococcenol A Derivatives: Design, Synthesis, and Cytotoxicity","authors":"Dídia Sousa, Milene A. G. Fortunato, Joana Silva, Mónica Pingo, Alice Martins, Carlos A. M. Afonso, Rui Pedrosa, Filipa Siopa, Celso Alves","doi":"10.3390/md22090408","DOIUrl":"https://doi.org/10.3390/md22090408","url":null,"abstract":"Sphaerococcenol A is a cytotoxic bromoditerpene biosynthesized by the red alga Sphaerococcus coronopifolius. A series of its analogues (1–6) was designed and semi-synthesized using thiol-Michael additions and enone reduction, and the structures of these analogues were characterized by spectroscopic methods. Cytotoxic analyses (1–100 µM; 24 h) were accomplished on A549, DU-145, and MCF-7 cells. The six novel sphaerococcenol A analogues displayed an IC50 range between 14.31 and 70.11 µM on A549, DU-145, and MCF-7 malignant cells. Compound 1, resulting from the chemical addition of 4-methoxybenzenethiol, exhibited the smallest IC50 values on the A549 (18.70 µM) and DU-145 (15.82 µM) cell lines, and compound 3, resulting from the chemical addition of propanethiol, exhibited the smallest IC50 value (14.31 µM) on MCF-7 cells. The highest IC50 values were exhibited by compound 4, suggesting that the chemical addition of benzylthiol led to a loss of cytotoxic activity. The remaining chemical modifications were not able to potentiate the cytotoxicity of the original compounds. Regarding A549 cell viability, analogue 1 exhibited a marked effect on mitochondrial function, which was accompanied by an increase in ROS levels, Caspase-3 activation, and DNA fragmentation and condensation. This study opens new avenues for research by exploring sphaerococcenol A as a scaffold for the synthesis of novel bioactive molecules.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"24 1","pages":""},"PeriodicalIF":5.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142211306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stress-Induced Production of Bioactive Oxylipins in Marine Microalgae 海洋微藻类在压力诱导下产生生物活性氧化脂

IF 5.4 2区医学

Marine Drugs Pub Date : 2024-09-04 DOI: 10.3390/md22090406

Amandyne Linares-Maurizi, Rana Awad, Anaelle Durbec, Guillaume Reversat, Valérie Gros, Jean-Marie Galano, Justine Bertrand-Michel, Thierry Durand, Rémi Pradelles, Camille Oger, Claire Vigor

{"title":"Stress-Induced Production of Bioactive Oxylipins in Marine Microalgae","authors":"Amandyne Linares-Maurizi, Rana Awad, Anaelle Durbec, Guillaume Reversat, Valérie Gros, Jean-Marie Galano, Justine Bertrand-Michel, Thierry Durand, Rémi Pradelles, Camille Oger, Claire Vigor","doi":"10.3390/md22090406","DOIUrl":"https://doi.org/10.3390/md22090406","url":null,"abstract":"Microalgae, stemming from a complex evolutionary lineage, possess a metabolic composition influenced by their evolutionary journey. They have the capacity to generate diverse polyunsaturated fatty acids (PUFAs), akin to those found in terrestrial plants and oily fish. Also, because of their numerous double bonds, these metabolic compounds are prone to oxidation processes, leading to the creation of valuable bioactive molecules called oxylipins. Moreover, owing to their adaptability across various environments, microalgae offer an intriguing avenue for biosynthesizing these compounds. Thus, modifying the culture conditions could potentially impact the profiles of oxylipins. Indeed, the accumulation of oxylipins in microalgae is subject to the influence of growth conditions, nutrient availability, and stressors, and adjusting these factors can enhance their production in microalgae culture. Consequently, the present study scrutinized the LC-MS/MS profiles of oxylipins from three marine microalgae species (two Haptagophytes and one Chlorophyte) cultivated in 1 L of photobioreactors under varying stress-inducing conditions, such as the introduction of H2O2, EtOAc, and NaCl, during their exponential growth phase. Approximately 50 oxylipins were identified, exhibiting different concentrations depending on the species and growth circumstances. This research suggests that microalgae metabolisms can be steered toward the production of bioactive oxylipins through modifications in the culture conditions. In this instance, the application of a low dose of hydrogen peroxide to Mi 124 appears to stimulate the production of nonenzymatic oxylipins. For Mi136, it is the application of salt stress that seems to increase the overall production of oxylipins. In the case of Mi 168, either a low concentration of H2O2 or a high concentration of AcOEt appears to have this effect.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"13 1","pages":""},"PeriodicalIF":5.4,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142211309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light Spectra, a Promising Tool to Modulate Ulva lacinulata Productivity and Composition 光谱--调节 Ulva lacinulata 生产率和组成的有效工具

IF 5.4 2区医学

Marine Drugs Pub Date : 2024-09-03 DOI: 10.3390/md22090404

Helena M. Amaro, Fernando Pagels, Rosa Melo, Antoine Fort, Ronan Sulpice, Graciliana Lopes, Isabel Costa, Isabel Sousa-Pinto

{"title":"Light Spectra, a Promising Tool to Modulate Ulva lacinulata Productivity and Composition","authors":"Helena M. Amaro, Fernando Pagels, Rosa Melo, Antoine Fort, Ronan Sulpice, Graciliana Lopes, Isabel Costa, Isabel Sousa-Pinto","doi":"10.3390/md22090404","DOIUrl":"https://doi.org/10.3390/md22090404","url":null,"abstract":"Light quality is a key factor affecting algal growth and biomass composition, particularly pigments such as carotenoids, known for their antioxidant properties. Light-emitting diodes (LEDs) are becoming a cost-effective solution for indoor seaweed production when compared to fluorescent bulbs, allowing full control of the light spectra. However, knowledge of its effects on Ulva biomass production is still scarce. In this study, we investigated the effects of LEDs on the phenotype of an Ulva lacinulata strain, collected on the Northern Portuguese coast. Effects of white (W), green (G), red (R), and blue (B) LEDs were evaluated for growth (fresh weight and area), photosynthetic activity, sporulation, and content of pigments and antioxidant compounds. The results showed that there were no significant differences in terms of fresh weight accumulation and reduced sporulation among the tested LEDs, while W light induced the highest expansion rate. Under G, U. lacinulata attained a quicker photoacclimation, and the highest content of pigments and total antioxidant activity; but with R and W, antioxidant compounds against the specific radicals O2•− and •NO were produced in a higher content when compared to other LEDs. Altogether, this study demonstrated that it is possible to modulate the bioactive properties of U. lacinulata by using W, R, and G light, opening the path to the production of biomass tailored for specific nutraceutical applications.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"50 1","pages":""},"PeriodicalIF":5.4,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142211370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marine Microorganism Molecules as Potential Anti-Inflammatory Therapeutics 作为潜在抗炎治疗药物的海洋微生物分子

IF 5.4 2区医学

Marine Drugs Pub Date : 2024-09-03 DOI: 10.3390/md22090405

Malia Lasalo, Thierry Jauffrais, Philippe Georgel, Mariko Matsui

引用次数: 0

New Sesquiterpenoids from the Mangrove-Derived Fungus Talaromyces sp. as Modulators of Nuclear Receptors 作为核受体调节剂的红树林真菌 Talaromyces sp.

IF 5.4 2区医学

Marine Drugs Pub Date : 2024-09-03 DOI: 10.3390/md22090403

Tanwei Gu, Jian Cai, Danni Xie, Jianglian She, Yonghong Liu, Xuefeng Zhou, Lan Tang

引用次数: 0

Anti-Inflammatory Effect of Fucoidan from Costaria costata Inhibited Lipopolysaccharide-Induced Inflammation in Mice 褐藻糖胶的抗炎作用可抑制脂多糖诱发的小鼠炎症

IF 5.4 2区医学

Marine Drugs Pub Date : 2024-09-02 DOI: 10.3390/md22090401

Wei Zhang, Peter C. W. Lee, Jun-O Jin

{"title":"Anti-Inflammatory Effect of Fucoidan from Costaria costata Inhibited Lipopolysaccharide-Induced Inflammation in Mice","authors":"Wei Zhang, Peter C. W. Lee, Jun-O Jin","doi":"10.3390/md22090401","DOIUrl":"https://doi.org/10.3390/md22090401","url":null,"abstract":"Seaweed extracts, especially fucoidan, are well known for their immune-modulating abilities. In this current study, we extracted fucoidan from Costaria costata, a seaweed commonly found in coastal Asia, and examined its anti-inflammatory effect. Fucoidan was extracted from dried C. costata (FCC) using an alcohol extraction method at an extraction rate of 4.5 ± 0.21%. The extracted FCC comprised the highest proportion of carbohydrates, along with sulfate and uronic acid. The immune regulatory effect of FCC was examined using bone marrow-derived dendritic cells (BMDCs). Pretreatment with FCC dose-dependently decreased the lipopolysaccharide (LPS)-induced upregulation of co-stimulatory molecules and major histocompatibility complex. In addition, FCC prevented morphological changes in LPS-induced BMDCs. Moreover, treatment of LPS-induced BMDCs with FCC suppressed the secretion of pro-inflammatory cytokines. In C57BL/6 mice, oral administration of FCC suppressed LPS-induced lung inflammation, reducing the secretion of pro-inflammatory cytokines in the bronchoalveolar lavage fluid. Finally, the administration of FCC suppressed LPS-induced sepsis. Therefore, FCC could be developed as a health supplement based on the observed anti-inflammatory effects.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"45 1","pages":""},"PeriodicalIF":5.4,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142211310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An Overview of Secondary Metabolites from Soft Corals of the Genus Capnella over the Five Decades: Chemical Structures, Pharmacological Activities, NMR Data, and Chemical Synthesis 五十年来 Capnella 属软珊瑚次生代谢物概述：化学结构、药理活性、核磁共振数据和化学合成

IF 5.4 2区医学

Marine Drugs Pub Date : 2024-09-02 DOI: 10.3390/md22090402

Can-Qi Liu, Qi-Bin Yang, Ling Zhang, Lin-Fu Liang

{"title":"An Overview of Secondary Metabolites from Soft Corals of the Genus Capnella over the Five Decades: Chemical Structures, Pharmacological Activities, NMR Data, and Chemical Synthesis","authors":"Can-Qi Liu, Qi-Bin Yang, Ling Zhang, Lin-Fu Liang","doi":"10.3390/md22090402","DOIUrl":"https://doi.org/10.3390/md22090402","url":null,"abstract":"There has been no specific review on the secondary metabolites from soft corals of the genus Capnella till now. In this work, all secondary metabolites from different species of the title genus were described. It covered the first work from 1974 to May 2024, spanning five decades. In the viewpoint of the general structural features, these chemical constituents were classified into four groups: sesquiterpenes, diterpenes, steroids, and lipids. Additionally, the 1H and 13C NMR data of these metabolites were provided when available in the literature. Among them, sesquiterpenes were the most abundant chemical compositions from soft corals of the genus Capnella. A variety of pharmacological activities of these compounds were evaluated, such as cytotoxic, antibacterial, antifungal, and anti-inflammatory activities. In addition, the chemical synthesis works of several representative sesquiterpenes were provided. This review aims to provide an up-to-date knowledge of the chemical structures, pharmacological activities, and chemical synthesis of the chemical constituents from soft corals of the genus Capnella.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"266 1","pages":""},"PeriodicalIF":5.4,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142211312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel Galectins Purified from the Sponge Chondrilla australiensis: Unique Structural Features and Cytotoxic Effects on Colorectal Cancer Cells Mediated by TF-Antigen Binding 从澳大利亚海绵中纯化的新型凝胶酶：由 TF 抗原结合介导的独特结构特征和对结直肠癌细胞的细胞毒性作用

IF 5.4 2区医学

Marine Drugs Pub Date : 2024-08-31 DOI: 10.3390/md22090400

Ryuhei Hayashi, Kenichi Kamata, Marco Gerdol, Yuki Fujii, Takashi Hayashi, Yuto Onoda, Nanae Kobayashi, Satoshi Furushima, Ryuya Ishiwata, Mayuka Ohkawa, Naoko Masuda, Yuka Niimi, Masao Yamada, Daisuke Adachi, Sarkar M. A. Kawsar, Sultana Rajia, Imtiaj Hasan, Somrita Padma, Bishnu Pada Chatterjee, Yuji Ise, Riku Chida, Kayo Hasehira, Nobumitsu Miyanishi, Tatsuya Kawasaki, Yukiko Ogawa, Hideaki Fujita, Alberto Pallavicini, Yasuhiro Ozeki

{"title":"Novel Galectins Purified from the Sponge Chondrilla australiensis: Unique Structural Features and Cytotoxic Effects on Colorectal Cancer Cells Mediated by TF-Antigen Binding","authors":"Ryuhei Hayashi, Kenichi Kamata, Marco Gerdol, Yuki Fujii, Takashi Hayashi, Yuto Onoda, Nanae Kobayashi, Satoshi Furushima, Ryuya Ishiwata, Mayuka Ohkawa, Naoko Masuda, Yuka Niimi, Masao Yamada, Daisuke Adachi, Sarkar M. A. Kawsar, Sultana Rajia, Imtiaj Hasan, Somrita Padma, Bishnu Pada Chatterjee, Yuji Ise, Riku Chida, Kayo Hasehira, Nobumitsu Miyanishi, Tatsuya Kawasaki, Yukiko Ogawa, Hideaki Fujita, Alberto Pallavicini, Yasuhiro Ozeki","doi":"10.3390/md22090400","DOIUrl":"https://doi.org/10.3390/md22090400","url":null,"abstract":"We here report the purification of a novel member of the galectin family, the β-galactoside-binding lectin hRTL, from the marine sponge Chondrilla australiensis. The hRTL lectin is a tetrameric proto-type galectin with a subunit molecular weight of 15.5 kDa, consisting of 141 amino acids and sharing 92% primary sequence identity with the galectin CCL from the congeneric species C. caribensis. Transcriptome analysis allowed for the identification of additional sequences belonging to the same family, bringing the total number of hRTLs to six. Unlike most other galectins, hRTLs display a 23 amino acid-long signal peptide that, according to Erdman degradation, is post-translationally cleaved, leaving an N-terminal end devoid of acetylated modifications, unlike most other galectins. Moreover, two hRTLs display an internal insertion, which determines the presence of an unusual loop region that may have important functional implications. The characterization of the glycan-binding properties of hRTL revealed that it had high affinity towards TF-antigen, sialyl TF, and type-1 N-acetyl lactosamine with a Galβ1-3 structure. When administered to DLD-1 cells, a colorectal carcinoma cell line expressing mucin-associated TF-antigen, hRTL could induce glycan-dependent cytotoxicity.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"106 1","pages":""},"PeriodicalIF":5.4,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142211365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0