Marine Drugs最新文献_第7页

Preparation of Acetylcholinesterase Inhibitory Peptides from Yellowfin Tuna Pancreas Using Moderate Ultrasound-Assisted Enzymatic Hydrolysis.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-09 DOI: 10.3390/md23020075

Pai Peng, Hui Yu, Meiting Xian, Caiye Qu, Zhiqiang Guo, Shuyi Li, Zhenzhou Zhu, Juan Xiao

{"title":"Preparation of Acetylcholinesterase Inhibitory Peptides from Yellowfin Tuna Pancreas Using Moderate Ultrasound-Assisted Enzymatic Hydrolysis.","authors":"Pai Peng, Hui Yu, Meiting Xian, Caiye Qu, Zhiqiang Guo, Shuyi Li, Zhenzhou Zhu, Juan Xiao","doi":"10.3390/md23020075","DOIUrl":"10.3390/md23020075","url":null,"abstract":"Bioactive peptides represent a promising therapeutic approach for Alzheimer's disease (AD) by maintaining cholinergic system homeostasis through the inhibition of acetylcholinesterase (AChE) activity. This study focused on extracting AChE inhibitory peptides from yellowfin tuna pancreas using moderate ultrasound-assisted enzymatic hydrolysis (MUE). Firstly, papain and MUE stood out from five enzymes and four enzymatic hydrolysis methods, respectively, by comparing the degree of hydrolysis and AChE inhibitory activity of different pancreatic protein hydrolysates. Subsequently, the optimal MUE conditions were obtained by single-factor, Plackett-Burman, and response surface methodologies. The pancreatic protein hydrolysate prepared under optimal MUE conditions was then purified by ultrafiltration followed by RP-HPLC, from which a novel AChE inhibitory peptide (LLDF) was identified by LC-MS/MS and virtual screening. LLDF effectively inhibited AChE activity by a competitive inhibition mechanism, with an IC50 of 18.44 ± 0.24 μM. Molecular docking and molecular dynamic simulation revealed that LLDF bound robustly to the active site of AChE via hydrogen bonds. These findings provided a theoretical basis for the valuable use of yellowfin tuna pancreas and introduced a new viewpoint on the potential therapeutic advantages of AChE inhibitory peptides for future AD treatment.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced Technologies for Large Scale Supply of Marine Drugs.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-07 DOI: 10.3390/md23020069

Henar Martínez, Mercedes Santos, Lucía Pedraza, Ana M Testera

{"title":"Advanced Technologies for Large Scale Supply of Marine Drugs.","authors":"Henar Martínez, Mercedes Santos, Lucía Pedraza, Ana M Testera","doi":"10.3390/md23020069","DOIUrl":"10.3390/md23020069","url":null,"abstract":"Marine organisms represent a source of unique chemical entities with valuable biomedical potentialities, broad diversity, and complexity. It is essential to ensure a reliable and sustainable supply of marine natural products (MNPs) for their translation into commercial drugs and other valuable products. From a structural point of view and with few exceptions, MNPs of pharmaceutical importance derive from the so-called secondary metabolism of marine organisms. When production strategies rely on marine macroorganisms, harvesting or culturing coupled with extraction procedures frequently remain the only alternative to producing these compounds on an industrial scale. Their supply can often be implemented with laboratory scale cultures for bacterial, fungal, or microalgal sources. However, a diverse approach, combining traditional methods with modern synthetic biology and biosynthesis strategies, must be considered for invertebrate MNPs, as they are usually naturally accumulated in only very small quantities. This review offers a comprehensive examination of various production strategies for MNPs, addressing the challenges related to supply, synthesis, and scalability. It also underscores recent biotechnological advancements that are likely to transform the current industrial-scale manufacturing methods for pharmaceuticals derived from marine sources.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anticancer Properties of Macroalgae: A Comprehensive Review.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-07 DOI: 10.3390/md23020070

Sara Frazzini, Luciana Rossi

{"title":"Anticancer Properties of Macroalgae: A Comprehensive Review.","authors":"Sara Frazzini, Luciana Rossi","doi":"10.3390/md23020070","DOIUrl":"10.3390/md23020070","url":null,"abstract":"In recent years, the exploration of bioactive molecules derived from natural sources has gained interest in several application fields. Among these, macroalgae have garnered significant attention due to their functional properties, which make them interesting in therapeutic applications, including cancer treatment. Cancer constitutes a significant global health burden, and the side effects of existing treatment modalities underscore the necessity for the exploration of novel therapeutic models that, in line with the goal of reducing drug treatments, take advantage of natural compounds. This review explores the anticancer properties of macroalgae, focusing on their bioactive compounds and mechanisms of action. The key findings suggest that macroalgae possess a rich array of bioactive compounds, including polysaccharides (e.g., fucoidans and alginates), polyphenols (e.g., phlorotannins), and terpenoids, which exhibit diverse anticancer activities, such as the inhibition of cell proliferation, angiogenesis, induction of apoptosis, and modulation of the immune system. This review provides an overview of the current understanding of macroalgae's anticancer potential, highlighting the most promising compounds and their mechanisms of action. While preclinical studies have shown promising results, further research is necessary to translate these findings into effective clinical applications.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TMF Attenuates Cognitive Impairment and Neuroinflammation by Inhibiting the MAPK/NF-κB Pathway in Alzheimer's Disease: A Multi-Omics Analysis.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-07 DOI: 10.3390/md23020074

Yonglin Liu, Xi Xu, Xiaoming Wu, Guodong Yang, Jiaxin Luo, Xinli Liang, Jie Chen, Yiguang Li

引用次数: 0

Marine-Derived Diterpenes from 2019 to 2024: Structures, Biological Activities, Synthesis and Potential Applications.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-07 DOI: 10.3390/md23020072

Lin Zhang, Debao Li, Xuan Chen, Feng Zhao

{"title":"Marine-Derived Diterpenes from 2019 to 2024: Structures, Biological Activities, Synthesis and Potential Applications.","authors":"Lin Zhang, Debao Li, Xuan Chen, Feng Zhao","doi":"10.3390/md23020072","DOIUrl":"10.3390/md23020072","url":null,"abstract":"Marine diterpenes are an important category of secondary metabolites derived from marine sources, found in a variety of marine animals, plants, and fungi. The increasing diversity of diterpene compounds, along with their unique chemical structures and specific biological activities, have attracted widespread attention. These activities include anti-inflammatory, antiviral, antitumor, antibacterial effects, and therapeutic actions against cancer, with many already developed into clinical drugs. Additionally, as marine medicinal resources continue to be exploited over the long term, the natural resources of many marine diterpene compounds are diminishing, and the biosynthesis of key active components has become a hot topic of research. In this review, we summarize diterpene compounds discovered in the ocean over the past five years, reclassify these compounds, and summarize their structures, biological activities, biosynthesis, and potential applications of marine diterpenoids from 2019 to 2024. This review can provide a reference for the basic research and potential applications of marine-derived diterpene compounds.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857513/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated Macrogenomics and Metabolomics Analysis of the Effect of Sea Cucumber Ovum Hydrolysates on Dextran Sodium Sulfate-Induced Colitis.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-07 DOI: 10.3390/md23020073

Shunmin Gong, Liqin Sun, Yongjun Sun, Wenming Ju, Gongming Wang, Jian Zhang, Xuejun Fu, Chang Lu, Yu Zhang, Wenkui Song, Mingbo Li, Leilei Sun

引用次数: 0

The Role of Brown Algae as a Capping Agent in the Synthesis of ZnO Nanoparticles to Enhance the Antibacterial Activities of Cotton Fabrics.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-07 DOI: 10.3390/md23020071

Eli Rohaeti, Helmiyati, Rasamimanana Joronavalona, Paulina Taba, Dewi Sondari, Azlan Kamari

{"title":"The Role of Brown Algae as a Capping Agent in the Synthesis of ZnO Nanoparticles to Enhance the Antibacterial Activities of Cotton Fabrics.","authors":"Eli Rohaeti, Helmiyati, Rasamimanana Joronavalona, Paulina Taba, Dewi Sondari, Azlan Kamari","doi":"10.3390/md23020071","DOIUrl":"10.3390/md23020071","url":null,"abstract":"Research was conducted on the role of brown algae as a capping agent in the synthesis of ZnO nanoparticles, the characteristics of ZnO nanoparticles, and the effect of the addition of ZnO nanoparticles and/or silane compounds on antibacterial and antifungal activities. The synthesis of ZnO nanoparticles involved green synthesis, and then nanoparticles were characterized using UV/VIS/NIR, ATR-FTIR, XRD, PSA, and SEM-EDS, followed by the in situ deposition of ZnO nanoparticles on cotton fabrics and the addition of silane compounds. The characterization of modified and unmodified cotton fabrics and antibacterial and antifungal activity tests were carried out using the disc diffusion method through measurements of the diameter of the inhibition zone against Pseudomonas aeruginosa, Staphylococcus epidermidis, and Malassezia furfur. The characterization of ZnO nanoparticles showed absorption at a wavelength of 357 nm; the number of waves was 450 cm-1; the diffraction peak occurred at an angle of 36.14°; the crystal size was 15.35 nm; there was a heterogeneous particle distribution; the particle size was in the ranges of 1.74-706 nm (PSA) and 45-297 nm (SEM); and an irregular particle shape was noted. The results showed that the best antibacterial and antifungal activity was obtained in cotton + HDTMS + ZnO nanoparticles (K8) and cotton + ZnO nanoparticles+HDTMS/MTMS (K4).","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857194/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Evaluation of the Antibacterial and Antitumor Activities of Marine Alkaloid 3,10-Dibromofascaplysin.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-06 DOI: 10.3390/md23020068

Maxim E Zhidkov, Polina A Smirnova, Natalia E Grammatikova, Elena B Isakova, Andrey E Shchekotikhin, Olga N Styshova, Anna A Klimovich, Aleksandr M Popov

{"title":"Comparative Evaluation of the Antibacterial and Antitumor Activities of Marine Alkaloid 3,10-Dibromofascaplysin.","authors":"Maxim E Zhidkov, Polina A Smirnova, Natalia E Grammatikova, Elena B Isakova, Andrey E Shchekotikhin, Olga N Styshova, Anna A Klimovich, Aleksandr M Popov","doi":"10.3390/md23020068","DOIUrl":"10.3390/md23020068","url":null,"abstract":"Fascaplysins form a group of marine natural products with unique cationic five-ring coplanar backbone. Native fascaplysin exhibits a broad spectrum of bioactivities, among which the cytotoxic activity has been the most investigated. Several fascaplysin derivatives have more selective biological effects and are promising as lead compounds. Thus, the introduction of a substituent at C-9 of fascaplysin leads to a strong increase in its antimicrobial properties. Here, a comparative assessment of the antimicrobial activity of synthetic analogs of the marine alkaloids 3-bromofascaplysin, 10-bromofascaplysin, and 3,10-dibromofascaplysin, along with some of their isomers and analogs, was carried out against a panel of Gram-positive bacteria in vitro. For the first time, a significant increase in the antimicrobial activity of fascaplysin was observed when a substituent was introduced at C-3. The introduction of two bromine atoms at C-2 and C-9 enhances the antimicrobial properties by 4 to 16 times, depending on the tested strain. Evaluation of the antimicrobial potential in vivo showed that fascaplysin and 3,10-dibromofascaplysin had comparable efficacy in the mouse staphylococcal sepsis model. Additionally, 3,10-dibromofascaplysin demonstrated a strong and reliable antitumor effect in vivo on the Ehrlich carcinoma inoculated subcutaneously, with a value of tumor growth inhibition by 49.2% 20 days after treatment. However, further studies on alternative chemical modifications of fascaplysin are needed to improve its chemotherapeutic properties.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First Confirmed Occurrence of Ciguatera Poisoning in the UK from Imported Pinjalo Snapper (Pinjalo pinjalo).

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-06 DOI: 10.3390/md23020067

Andrew D Turner, Benjamin H Maskrey, David Stone, Elizabeth M Mudge, Alison Robertson

{"title":"First Confirmed Occurrence of Ciguatera Poisoning in the UK from Imported Pinjalo Snapper (Pinjalo pinjalo).","authors":"Andrew D Turner, Benjamin H Maskrey, David Stone, Elizabeth M Mudge, Alison Robertson","doi":"10.3390/md23020067","DOIUrl":"10.3390/md23020067","url":null,"abstract":"Three people in England consumed fish steaks labeled as Red Snapper (Lutjanus bohar) originating from the Indian Ocean. Within 12 h, all three experienced sickness including nausea, vomiting, diarrhea, as well as myalgia and paresthesia. Three steaks from a single package of fish obtained from a grocery store were consumed, leaving one uneaten, which was submitted for analysis. Cytotoxicity testing via the mouse neuroblastoma assay confirmed the presence of sodium channel specific activity consistent with a ciguatoxin standard, and the levels detected were above established guidance limits for safe consumption. Chemical detection using liquid chromatography coupled with high-resolution mass spectrometry of both intact toxins and periodate oxidation products was used to confirm the presence of chromatographic peaks consistent with tri- and di-hydroxylated Pacific ciguatoxin 3C congeners. Taking the shared medical symptoms of patients, the recent dietary history, and the known potential for ciguatera poisoning to occur in snapper species, the subsequent evidence for CTX-like activity and CTXs in the same fish sample provides very strong evidence that the fish steaks consumed were similarly contaminated with CTXs. Furthermore, given the levels reported, such toxicity would be expected to cause intoxication in humans. Fish species identification based on DNA barcoding confirmed that the fish products were mislabeled, with the tissues instead being the Pinjalo snapper, Pinjalo pinjalo. This is the first confirmed ciguatera poisoning incident in both the UK and from the Pinjalo snapper and highlights the need for monitoring of these emerging toxins in reef fish imports to prevent future human intoxication.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA Editing Analysis Reveals Methyl Jasmonic Acid Regulation of Fucoxanthin and Fatty Acid Metabolism in Phaeodactylum tricornutum.

IF 4.9 2区医学

Marine Drugs Pub Date : 2025-02-06 DOI: 10.3390/md23020066

Sihui Huang, Hao Liu, Ruihao Xu, Wangchang Li, Han Yang, Xinlei Bao, Yuqing Hang, Yifu Gong, Yuxiang Zhao

{"title":"RNA Editing Analysis Reveals Methyl Jasmonic Acid Regulation of Fucoxanthin and Fatty Acid Metabolism in Phaeodactylum tricornutum.","authors":"Sihui Huang, Hao Liu, Ruihao Xu, Wangchang Li, Han Yang, Xinlei Bao, Yuqing Hang, Yifu Gong, Yuxiang Zhao","doi":"10.3390/md23020066","DOIUrl":"10.3390/md23020066","url":null,"abstract":"Phaeodactylum tricornutum is a marine diatom with significant biotechnological potential, particularly in producing high-value bioactive compounds such as fucoxanthin and unsaturated fatty acids, which possess significant pharmaceutical and nutraceutical properties. However, the naturally low yields of these compounds present a major challenge for large-scale production. Methyl jasmonic acid (MeJA), a plant-derived signaling molecule, has been shown to enhance the biosynthesis of these metabolites in P. tricornutum. While transcriptional regulation has been extensively studied, the role of post-transcriptional modifications, such as RNA editing, in mediating MeJA-induced metabolic changes remains largely unexplored. RNA editing can alter nucleotide sequences, leading to functional changes in gene expression and protein activity, thus providing a potential regulatory mechanism for enhanced biosynthesis of target metabolites. In this study, we investigated the role of RNA editing in Phaeodactylum tricornutum under methyl jasmonic acid (MeJA) treatment, focusing on its impact on the accumulation of bioactive compounds such as fucoxanthin and fatty acids. We conducted a comprehensive comparative analysis of RNA editing events across MeJA-treated and control groups. Our findings reveal that MeJA treatment induces significant variations in RNA editing levels, affecting key metabolic pathways. Notably, two genes, Lhcr10 (Phatr3_J16481) and Phatr3_J43665, were identified as potential contributors to increased RNA editing enzyme activity and to energy metabolism and fatty acid biosynthesis under MeJA treatment. These results provide a foundation for the discovery of molecular mechanisms underlying adaptive responses in P. tricornutum and highlight RNA editing as a critical regulatory mechanism in MeJA-induced metabolic reprogramming.","PeriodicalId":18222,"journal":{"name":"Marine Drugs","volume":"23 2","pages":""},"PeriodicalIF":4.9,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11857586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0