Lasers in Medical Science最新文献

{"title":"Photobiomodulation associated to a 58S bioactive glass for dentin hypersensitivity treatment - an in vitro study on differentiation of human dental pulp cells.","authors":"Ítallo Emídio Lira Viana, Juliana Marchi, Sabrina Sochacki, Márcia Martins Marques, Carla Renata Sipert, Taís Scaramucci","doi":"10.1007/s10103-025-04633-2","DOIUrl":"https://doi.org/10.1007/s10103-025-04633-2","url":null,"abstract":"<p><p>To analyze the effects of a 58S experimental bioactive glass associated or not to photobiomodulation (PBM) therapy on the functional differentiation of human dental pulp cells (HDPCs). Dentin discs (1 mm-thick) were mounted on metallic devices, simulating the dental pulp chamber, positioned over HDPC cultures, and the following treatments were applied to the dentin discs, associated or not to PBM (InGaAlP - 660 nm, 5 J/cm², continuous mode, punctual and in contact): BGP - experimental bioactive glass paste; NP - Nupro prophylaxis paste; CXT - Clinpro XT varnish; -control (α-MEM); and +control (Differentiation Medium - DM). After 7, 14 and 21 days, calcium deposition was assessed by the Alizarin Red S staining assay, followed by elution and quantification of calcium, using spectrophotometry. RT-qPCR was used to assess the gene expression for BGLAP, SPP-1, DMP-1, DSPP and IBSP in the negative and positive control, BGP and BGP + PBM groups after 3 days. Data were statistically analyzed (α = 0.05). No significant differences between the experimental groups were found (p = 0.397) at 7 days. BGP group showed greater mineralization potential, differing from α-MEM (p < 0.05), but not from the other groups at 14 days. After 21 days, the BGP, DM, BGP + PBM and CXT groups showed higher mineralization potential, with no significant differences. Regarding gene expression, no significant differences were found for any of the targets tested. The experimental bioactive glass paste and Clinpro XT varnish showed potential for the formation of mineralized nodules after 14 and 21 days, regardless of photobiomodulation.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"386"},"PeriodicalIF":2.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of low-fluence 1064 nm picosecond laser with/without micro-lens arrays for melasma treatment: a randomized, split-face controlled study.","authors":"Xianglei Wu, Xue Wang, Xiaoxi Lin, Ying Shang, Qingqing Cen, Zhen Zhang","doi":"10.1007/s10103-025-04655-w","DOIUrl":"10.1007/s10103-025-04655-w","url":null,"abstract":"<p><p>Low-fluence picosecond (LFPS) neodymium-doped yttrium aluminum garnet (Nd: YAG) 1064 nm laser has emerged as a promising treatment for melasma; however, its optimal strategy remains unclear. To evaluate the efficacy and safety of LFPS combined with microlens arrays (MLA) compared with LFPS alone in treating melasma. Thirty patients with melasma received LFPS alone on one side of the face (control [C] side) and LFPS with MLA on the other (treatment [T] side). Treatments were performed at four-week intervals for five sessions. Standardized imaging, VISIA-CR, and assessments using the Physician's Global Aesthetic Improvement Scale (P-GAIS) and the Melasma Area and Severity Index (MASI) score, were performed at baseline, and at one-, three-, and six-months post-treatment. Recurrences and adverse effects were recorded. MASI scores significantly reduced at all time points compared with baseline (ΔMASI: 3.23, 3.35, and 2.98, respectively). The P-GAIS assessment showed that 3 patients rated 1 point, 5 rated 2, 14 rated 3, and 8 rated 4. No significant adverse events occurred. At six months, recurrence was lower on the T side than on the C side (1 vs. 6 patients; p< 0.05). VISIA-CR analysis showed significant spot reduction on both sides, with no statistically significant difference (p>0.05). Additionally, texture and pore metrics improved on the T side, whereas no significant changes were observed on the C side. LFPS with MLA improved melasma severity, reduced recurrence rates, and enhanced skin texture and pores compared with LFPS alone, offering a safe, effective treatment option.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"387"},"PeriodicalIF":2.4,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation by infrared radiation on hallmarks of cancer.","authors":"Ana Caroline da Silva Ferreira, Thayssa Gomes Farias, Ana Julia Cardoso Borges, Marcelle Abreu da Silva, Bruno Ricardo Barreto Pires, Andre Luiz Mencalha, Adenilson de Souza da Fonseca","doi":"10.1007/s10103-025-04625-2","DOIUrl":"https://doi.org/10.1007/s10103-025-04625-2","url":null,"abstract":"<p><p>Photobiomodulation (PBM) is a therapeutic technique that utilizes non-ionizing radiation, such as infrared radiation, which is capable of stimulating or inhibiting biological processes. Cancer is characterized by uncontrolled cell growth and proliferation in any body structure, allowing it to invade other tissues. Cancer exhibits distinct biological characteristics, the so-called hallmarks of cancer. Despite the therapeutic benefits of PBM, and cancers affect millions of people worldwide, the mechanisms involved in PBM, as those induced by infrared A radiation, on cancer are not yet fully elucidated. Thus, we aimed to review the effects of PBM induced by infrared radiation on the hallmarks of cancer. For this, studies on the effects of PBM induced by infrared A radiation on in vitro and in vivo experimental models of cancer were accessed via MEDLINE/PubMed. The data accessed showed that effects of PBM induced by infrared A radiation were evaluated on four hallmarks of cancer (sustaining proliferative signaling, evading growth suppressors, genomic instability and mutation, and resisting cell death) but not on the other ten ones (enabling replicative immortality, non-mutational epigenetic reprogramming, avoiding immune destruction, tumor-promoting inflammation, polymorphic microbiomes, activating invasion and metastasis, inducing or accessing vasculature, senescent cells, deregulation cellular energetics, unlocking phenotypic plasticity). Thus, considering the therapeutic benefits of PBM, evaluation of the effects of PBM on the hallmarks of cancer can also bring such benefits to oncologic patients.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"385"},"PeriodicalIF":2.4,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review on advancements in laser-assisted dental treatments: focus on minimally invasive techniques and clinical outcomes.","authors":"Sirus Safaee, Amirhossein Moghanian, Mohammad Mehrdar, Parviz Asadi, Mostafa Akbari, Mahyar Nesabi, Mahdis Nesabi","doi":"10.1007/s10103-025-04624-3","DOIUrl":"https://doi.org/10.1007/s10103-025-04624-3","url":null,"abstract":"<p><p>This review examines advancements in laser-assisted dental procedures, focusing on their applications, clinical outcomes, and the shift towards minimally invasive techniques. It discusses the fundamentals of laser technology, including wavelength selection, monochromaticity, coherence, and beam focusing, which enhance the precision and efficacy of treatment. A comprehensive literature search was conducted using multiple databases, including PubMed, Scopus, Web of Science, Google Scholar, and Embase, using keywords related to lasers in dentistry. Peer-reviewed articles, original research studies, clinical trials, and reviews on clinical applications, benefits, and limitations were included, whereas irrelevant studies were excluded. During the review process, we utilized a broad range of search engines to ensure the inclusion of the most up-to-date and comprehensive evidence reflecting the evolution of laser-tissue interactions. The integration of laser technology in dental practice represents a significant advancement, offering benefits such as increased precision in targeting specific areas, minimal damage to surrounding tissues, and reduced patient discomfort. Laser procedures typically result in less bleeding and swelling, often decreasing the need for anesthesia, which leads to a more comfortable patient experience. Additionally, the minimally invasive nature of lasers supports the quicker recovery and preservation of healthy tissues. This review highlights the diverse applications of lasers in dentistry, including caries detection, soft and hard tissue surgeries, periodontal therapy, and dentin hypersensitivity (DH) management, while addressing challenges such as equipment costs, training requirements, and safety considerations. The review also emphasizes the need to consider the broad spectrum of laser wavelengths currently used in clinical dentistry, beyond the commonly referenced Er: YAG, to provide a complete perspective of contemporary practice. Further research is needed to enhance the clinical integration, accessibility, and optimization of laser-based therapies.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"382"},"PeriodicalIF":2.4,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of photobiomodulation therapy with conventional treatment in patients with herpes labialis: a systematic review and meta-analysis of randomized controlled trials.","authors":"Qiong Yang, Wajiha Farooq, Muhammad Haroon, Syed Asadullah, Humam Shah, Mustafa Jawad Kadham, Iftikhar Ahmad","doi":"10.1007/s10103-025-04648-9","DOIUrl":"10.1007/s10103-025-04648-9","url":null,"abstract":"<p><p>Herpes labialis (HL) is a common viral infection of the lips, primarily caused by herpes simplex virus type1. Although conventional treatment for HL primarily consist of antiviral agents, photobiomodulation therapy (PBMT) has also emerged as an effective intervention. This study aims to compare PBMT with conventional treatment for HL. A systematic review of randomized controlled trials (RCTs) was conducted through Cochrane Library, PubMed, Semantic Scholar, Dimension AI and Web of Science in accordance to PRISMA guidelines. English language RCTs reporting comparison of PBMT with conventional treatment (i.e., acyclovir, valacyclovir, and penciclovir) in HL were eligible, without restriction on patient's age and publication year. The primary outcomes were pain (on visual analog scale: VAS), healing time (in days) and HL lesion size (in cm²). Cochrane's Risk of Bias (RoB) 2.0 tool and RevMan 5.0 was used for quality appraisal and conducting meta-analysis of the selected RCTs, respectively. Six RCTs involving ²60 HL patients (PBMT: 129, conventional treatment: 131) were included. Pain score at day 2 and 3 after treatment showed an overall effect favoring the PBMT with a standardized mean difference (SMD) of [-1.54 (95% CI: -2.32 to -0.77; Z = 3.92, p < 0.001] and [-0.88 (95% CI: -1.19 to -0.57; Z = 5.62, p < 0.001], respectively. The pooled data revealed that PBMT resulted in significantly faster healing of the HL lesions [1.22 days (95% CI: -1.93 to -0.51; p = 0.0007). At day 3 after treatment, a significantly higher decrease in the lesion size was observed for patients receiving PBMT compared conventional treatment [-0.99 cm² (95% CI: -1.41 to -057; p < 0.0001)]. The overall RoB score for 05 RCTs revealed High risk of bias, primarily arising from domain 5 of the RoB 2.0 tool. PBMT demonstrated significant reduction in pain, healing time and lesion size, as compared to conventional treatment, though smaller number of available RCTs warrants further investigation.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"381"},"PeriodicalIF":2.4,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effectiveness of photobiomodulation therapy on bone regeneration of oral and craniofacial defects. A systematic review of animal, and in-vitro studies.","authors":"Maryam Saki, Shabnam Tahamtan, Mojgan Shavakhi, Kinga Grzech-Leśniak, Reza Fekrazad","doi":"10.1007/s10103-025-04617-2","DOIUrl":"10.1007/s10103-025-04617-2","url":null,"abstract":"<p><p>Photobiomodulation Therapy (PBMT) is a noninvasive approach that utilizes light application to accelerate healing, promote tissue repair, reduce inflammation and pain. This systematic review aims to investigate the effects of PBMT on craniofacial bone regeneration by examining the available literature. A comprehensive electronic search was conducted across multiple databases including PubMed, EMBASE, Web of Science, Scopus, and Cochrane. The initial analysis focused on animal studies that reported the effects of PBMT on bone regeneration and healing in animals. Additionally, in vitro histological studies were considered in this investigation. The quality assessment of all included animal studies was performed by two investigators using the Systematic Review Centre for Laboratory Animal Experimentation (SYRCLE) Tool. A total of 60 studies were included in this systematic review, meeting the eligibility criteria. Among them, 43 studies were conducted on animals, while 18 studies involved in vitro histological analysis. The reviewed studies exhibited significant variations in terms of irradiation parameters, experimental models, execution methods, and cell types utilized. This heterogeneity presents challenges in establishing standardized clinical guidelines for laser therapy. However, the findings suggest that PBMT has the potential to positively impact bone formation, mineralization, angiogenesis, osteoblast differentiation, and tissue remodeling. PBMT has shown effectiveness in enhancing the repair processes of craniofacial bone defects. However, it is important to consider that the presence of biomaterials may temporarily hinder the effects of PBMT. Standardization is still flawed, and it should be better studied to allow more specific conclusions in relation to PBMT use protocols.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"384"},"PeriodicalIF":2.4,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive systematic review on the efficacy, safety, tolerability, and relapse rates of modern procedural therapies for inflammatory and non-inflammatory acne vulgaris.","authors":"Arash Pour Mohammad, Farnoosh Seirafianpour, Alireza Jafarzadeh, Sara Ghahari, Elahe Noroozi, Amir Hossein Nazerian, Ali Jamshidi Naeini, Kamyar Shokraee, Farzaneh Mashayekhi, Mahsa Mortaja, Arian Azmi, Azadeh Goodarzi","doi":"10.1007/s10103-025-04640-3","DOIUrl":"https://doi.org/10.1007/s10103-025-04640-3","url":null,"abstract":"<p><p>Procedural therapies are increasingly recognized as effective adjunctive treatments for acne vulgaris alongside topical and systemic options. This systematic review aims to provide a comprehensive overview of the efficacy, safety and relapse rate of various procedural therapies in managing acne vulgaris. Data from 160 studies involving 8121 patients were analyzed across four major databases up to 2024. Treatment approaches were stratified into six categories: Light therapies (n = 55), Chemical Peelings (n = 20), Lasers (n = 21), Radiofrequency (n = 5), Comparative Studies (n = 18), Combination Studies (n = 36), and other modalities (n = 5). Photodynamic therapy (PDT) using Intense Pulsed Light and Aminolaevulinic acid showed a 56.66% median improvement rate for inflammatory acne but had low tolerability due to pain and burning sensations, Limiting its use.Among chemical peels, Salicylic Acid at 30% had the highest efficacy at 85%, followed by Glycolic Acid at 80.25%. In contrast, Jessner Solution and 25%Trichloroacetic acid were less effective and increased the risk of post-inflammatory hyperpigmentation (PIH).Comparing lasers and light therapies showed that PDL had less efficacy compared to light therapies, but Nd:YAG was more effective, especially in noninflammatory lesions, with fewer flare-ups. Fractional Microneedling Radiofrequency (RF) was more effective and had lower recurrence rates in comparison to lasers and RF. The evolution in acne vulgaris management highlights the growing prominence of procedural therapies, each offering varying levels of efficacy and safety profiles.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"383"},"PeriodicalIF":2.4,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145138022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of non-ablative vaginal erbium laser for rUTI prevention in postmenopausal women.","authors":"Jiqiong Zheng, Minyan Li, Yezi Chen, Zhengwang Zhang","doi":"10.1007/s10103-025-04637-y","DOIUrl":"10.1007/s10103-025-04637-y","url":null,"abstract":"<p><p>To evaluate the effect of vaginal erbium laser (VEL) for recurrent urinary tract infection (rUTI) prevention in postmenopausal women (PMW). 80 PMW with past histories of rUTI were recruited and randomized into local estrogen therapy (LET) or VEL group. The LET group was prescribed 12 weeks of vaginal estrogen, while treatment in the VEL group consisted of three VEL sessions at a 30-day interval. Primary outcomes were the number of UTI episodes and the percentage of cured and improved participants. Vaginal pH, lactobacillus flora, and vaginal health index score (VHIS) were assessed as secondary outcomes. The actual number of cumulative UTI episodes in VEL and LET were significantly lower than predictive numbers. No significant difference was found in the percentages of cured and improved participants between the two groups. Persistent improvement of vaginal health was observed in VEL. Only 54.8% of participants in LET still maintained vaginal lactobacillus predominance at the follow-up endpoint compared with 93.3% in VEL (p = 0.002). Consistently and significantly lower vaginal pH and higher VHIS scores were observed in VEL compared to LET at the same time-points. In comparison to vaginal estrogen, VEL showed a non-inferior and more sustained effect for rUTI prevention in PMW.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"380"},"PeriodicalIF":2.4,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation with LED does not alter antibiotic sensitivity in ATCC and burn-patient bacteria.","authors":"Flávia Fernanda de Oliveira Assunção, Lucas Chaves, Érika Nascimento, Alessandro Marcio Hakme Silva, Thiago Domingues Stocco, Vitor Hugo Balasco Serrão, Roberto Martines, Elaine Caldeira de Oliveira Guirro, Alceu Afonso Jordão Júnior, Rinaldo Roberto de Jesus Guirro","doi":"10.1007/s10103-025-04642-1","DOIUrl":"https://doi.org/10.1007/s10103-025-04642-1","url":null,"abstract":"<p><strong>Introduction: </strong>The association of photobiomodulation (PBM) and antibiotics has been little investigated for bacterial control.</p><p><strong>Objectives: </strong>This study aimed to verify possible changes in the antibiotic sensitivity profile in ATCC-type bacteria and bacteria collected from burned patients after irradiation with 465 and 630 nm LED at 30, 40 and 50 J/cm².</p><p><strong>Methods: </strong>The species Staphylococcus aureus (ATCC^®BAA-977), Pseudomonas aeruginosa (ATCC 27853), and Staphylococcus aureus and Pseudomonas aeruginosa from burned patients were used as proof of concept. A qualitative method was adopted to verify the antibiotic sensitivity profile. To quantify oxidative stress, assessments were performed using Malondialdehyde (MDA) and antioxidant action using glutathione (GSH). Data were analyzed by Shapiro-Wilk and ANOVA single-factor tests, with Tukey post-hoc (p < 0.05).</p><p><strong>Results: </strong>Differences between the irradiated groups were found, however, without statistical significance or representing a change in the sensitivity profile of the bacteria investigated. The highest production of oxidative stress was at 465 nm (30 J/cm²), and no glutathione production was detected.</p><p><strong>Conclusion: </strong>LED irradiation was found to be safe for application to bacterial colonies, as it did not modify their antibiotic susceptibility profile, despite inducing oxidative stress.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"379"},"PeriodicalIF":2.4,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145124964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical efficacy of conbercept combined with micropulse laser in patients with polypoidal choroidal vasculopathy.","authors":"Qingyan Liu, Yao Lu, Shuo Sun, Xiao Shao, Xin Yin, Shuying Ma, Min Xue","doi":"10.1007/s10103-025-04619-0","DOIUrl":"10.1007/s10103-025-04619-0","url":null,"abstract":"<p><p>To evaluate the clinical efficacy of conbercept combined with micropulse laser(MPL) in treating polypoidal choroidal vasculopathy (PCV). In this prospective, randomized controlled trial conducted from February 2023 to April 2024, 52 patients (52eyes) with PCV at Anhui NO.2 Provincial People's hospital were enrolled. Participants were randomized into a conbercept monotherapy group (27cases, 27eyes) and a combination treatment group (conbercept plus micropulse laser(MPL); 25cases, 25eyes). After an initial series of three intravitreal injections, all patients followed the Treat & Extend (T&E) protocol. Aqueous humor samples were collected before each of the first three injections, and cytokines levels were measured after the sample collection. The combined treatment group received a 577 nm MPL application two weeks following the first injection. The best corrected visual acuity (BCVA) 、central macular thickness (CMT) and total macular volume (TMV) of each group were assessed monthly for three months. From the first to the third month post-injection, both treatment groups showed improvements in BCVA and reductions in CMT and TMV, with the combined treatment group demonstrating significantly greater improvements at each monthly evaluation (P < 0.05). Additionally, levels of aqueous humor cytokines, including VEGF-A, IL-5, MCP-1, and Ang-2, were significantly reduced in both groups. Reductions in VEGF-A and MCP-1 concentrations were positively correlated with improvements in BCVA, CMT, and TMV (P < 0.05). Conbercept combined with MPL therapy demonstrates superior clinical efficacy compared to intravitreal injection of Conbercept alone in patients with PCV. Furthermore, changes in VEGF-A and MCP-1 levels may serve as predictive markers for improvements in visual acuity and retinal morphology in these patients.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"378"},"PeriodicalIF":2.4,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}