Flávia Fernanda de Oliveira Assunção, Lucas Chaves, Érika Nascimento, Alessandro Marcio Hakme Silva, Thiago Domingues Stocco, Vitor Hugo Balasco Serrão, Roberto Martines, Elaine Caldeira de Oliveira Guirro, Alceu Afonso Jordão Júnior, Rinaldo Roberto de Jesus Guirro
{"title":"LED光生物调节不会改变ATCC和烧伤患者细菌的抗生素敏感性。","authors":"Flávia Fernanda de Oliveira Assunção, Lucas Chaves, Érika Nascimento, Alessandro Marcio Hakme Silva, Thiago Domingues Stocco, Vitor Hugo Balasco Serrão, Roberto Martines, Elaine Caldeira de Oliveira Guirro, Alceu Afonso Jordão Júnior, Rinaldo Roberto de Jesus Guirro","doi":"10.1007/s10103-025-04642-1","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The association of photobiomodulation (PBM) and antibiotics has been little investigated for bacterial control.</p><p><strong>Objectives: </strong>This study aimed to verify possible changes in the antibiotic sensitivity profile in ATCC-type bacteria and bacteria collected from burned patients after irradiation with 465 and 630 nm LED at 30, 40 and 50 J/cm<sup>2</sup>.</p><p><strong>Methods: </strong>The species Staphylococcus aureus (ATCC<sup>®</sup>BAA-977), Pseudomonas aeruginosa (ATCC 27853), and Staphylococcus aureus and Pseudomonas aeruginosa from burned patients were used as proof of concept. A qualitative method was adopted to verify the antibiotic sensitivity profile. To quantify oxidative stress, assessments were performed using Malondialdehyde (MDA) and antioxidant action using glutathione (GSH). Data were analyzed by Shapiro-Wilk and ANOVA single-factor tests, with Tukey post-hoc (p < 0.05).</p><p><strong>Results: </strong>Differences between the irradiated groups were found, however, without statistical significance or representing a change in the sensitivity profile of the bacteria investigated. The highest production of oxidative stress was at 465 nm (30 J/cm<sup>2</sup>), and no glutathione production was detected.</p><p><strong>Conclusion: </strong>LED irradiation was found to be safe for application to bacterial colonies, as it did not modify their antibiotic susceptibility profile, despite inducing oxidative stress.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"379"},"PeriodicalIF":2.4000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Photobiomodulation with LED does not alter antibiotic sensitivity in ATCC and burn-patient bacteria.\",\"authors\":\"Flávia Fernanda de Oliveira Assunção, Lucas Chaves, Érika Nascimento, Alessandro Marcio Hakme Silva, Thiago Domingues Stocco, Vitor Hugo Balasco Serrão, Roberto Martines, Elaine Caldeira de Oliveira Guirro, Alceu Afonso Jordão Júnior, Rinaldo Roberto de Jesus Guirro\",\"doi\":\"10.1007/s10103-025-04642-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>The association of photobiomodulation (PBM) and antibiotics has been little investigated for bacterial control.</p><p><strong>Objectives: </strong>This study aimed to verify possible changes in the antibiotic sensitivity profile in ATCC-type bacteria and bacteria collected from burned patients after irradiation with 465 and 630 nm LED at 30, 40 and 50 J/cm<sup>2</sup>.</p><p><strong>Methods: </strong>The species Staphylococcus aureus (ATCC<sup>®</sup>BAA-977), Pseudomonas aeruginosa (ATCC 27853), and Staphylococcus aureus and Pseudomonas aeruginosa from burned patients were used as proof of concept. A qualitative method was adopted to verify the antibiotic sensitivity profile. To quantify oxidative stress, assessments were performed using Malondialdehyde (MDA) and antioxidant action using glutathione (GSH). Data were analyzed by Shapiro-Wilk and ANOVA single-factor tests, with Tukey post-hoc (p < 0.05).</p><p><strong>Results: </strong>Differences between the irradiated groups were found, however, without statistical significance or representing a change in the sensitivity profile of the bacteria investigated. The highest production of oxidative stress was at 465 nm (30 J/cm<sup>2</sup>), and no glutathione production was detected.</p><p><strong>Conclusion: </strong>LED irradiation was found to be safe for application to bacterial colonies, as it did not modify their antibiotic susceptibility profile, despite inducing oxidative stress.</p>\",\"PeriodicalId\":17978,\"journal\":{\"name\":\"Lasers in Medical Science\",\"volume\":\"40 1\",\"pages\":\"379\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Lasers in Medical Science\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s10103-025-04642-1\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lasers in Medical Science","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s10103-025-04642-1","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
Photobiomodulation with LED does not alter antibiotic sensitivity in ATCC and burn-patient bacteria.
Introduction: The association of photobiomodulation (PBM) and antibiotics has been little investigated for bacterial control.
Objectives: This study aimed to verify possible changes in the antibiotic sensitivity profile in ATCC-type bacteria and bacteria collected from burned patients after irradiation with 465 and 630 nm LED at 30, 40 and 50 J/cm2.
Methods: The species Staphylococcus aureus (ATCC®BAA-977), Pseudomonas aeruginosa (ATCC 27853), and Staphylococcus aureus and Pseudomonas aeruginosa from burned patients were used as proof of concept. A qualitative method was adopted to verify the antibiotic sensitivity profile. To quantify oxidative stress, assessments were performed using Malondialdehyde (MDA) and antioxidant action using glutathione (GSH). Data were analyzed by Shapiro-Wilk and ANOVA single-factor tests, with Tukey post-hoc (p < 0.05).
Results: Differences between the irradiated groups were found, however, without statistical significance or representing a change in the sensitivity profile of the bacteria investigated. The highest production of oxidative stress was at 465 nm (30 J/cm2), and no glutathione production was detected.
Conclusion: LED irradiation was found to be safe for application to bacterial colonies, as it did not modify their antibiotic susceptibility profile, despite inducing oxidative stress.
期刊介绍：
Lasers in Medical Science (LIMS) has established itself as the leading international journal in the rapidly expanding field of medical and dental applications of lasers and light. It provides a forum for the publication of papers on the technical, experimental, and clinical aspects of the use of medical lasers, including lasers in surgery, endoscopy, angioplasty, hyperthermia of tumors, and photodynamic therapy. In addition to medical laser applications, LIMS presents high-quality manuscripts on a wide range of dental topics, including aesthetic dentistry, endodontics, orthodontics, and prosthodontics.
The journal publishes articles on the medical and dental applications of novel laser technologies, light delivery systems, sensors to monitor laser effects, basic laser-tissue interactions, and the modeling of laser-tissue interactions. Beyond laser applications, LIMS features articles relating to the use of non-laser light-tissue interactions.
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