Lasers in Medical Science最新文献

{"title":"Optimisation of low-level light therapy (lllt) parameters and evaluation of its immunomodulatory effect on fibroblasts.","authors":"Anna Ścisłowska-Czarnecka, Amelia Lizak, Aleksandra Matuła, Aneta Bac, Joanna Homa, Magdalena Chadzińska, Beata Stenka","doi":"10.1007/s10103-026-04873-w","DOIUrl":"https://doi.org/10.1007/s10103-026-04873-w","url":null,"abstract":"<p><p>Low-level laser therapy (LLLT) is being increasingly applied in regenerative medicine due to its ability to modulate cellular metabolism and support repair processes. However, the effect of various exposure parameters on fibroblast activity still does not remain fully understood. The study aim was to evaluate the effect of selected LLLT parameters (808 nm, 100 and 200 mW, 2 and 10 J/cm², pulsed beam) on the biological activity of fibroblasts in vitro. Fibroblasts were exposed to the established exposure parameters, and then the number of adherent and metabolically active cells, their migration, adenylate kinase (AK) as well as nitric oxide (NO) levels, cytokine secretion, metalloproteinase (MMP-2, MMP-9) activity, and oxidative-antioxidant potential were assessed. The most favourable effects were achieved with 200 mW; 2 J/cm² and 100 m; 10 J/cm² exposure parameters. In these conditions, increased number of adherent and metabolically active cells, and elevated migration were observed. Moreover, in this condition decreased AK release and NO secretion were found. Furthermore, LLLT reduced in fibroblasts interleukin 6 (IL-6) and interleukin 8 (IL-8) secretion as well as affected the activity of metalloproteinases, what corresponded with subsequent wound healing process. Additionally, a beneficial effect on maintaining the oxidative-antioxidant balance in fibroblasts was noted. In summary, the effects of LLLT on fibroblasts depend on the applied irradiation parameters, and appropriately selected LLLT treatment (200 mW; 2 J/cm² and 100 mW; 10 J/cm²,) stabilise fibroblast metabolism, limits their proinflammatory response and promotes extracellular matrix remodelling (ECM). These results confirm the potential of LLLT as an effective method supporting regenerative processes in the treatment of tissue damage.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research advances in the pathogenesis and photodynamic therapy of pathological scars.","authors":"Jingyu Liu, Hongxiang Chen","doi":"10.1007/s10103-026-04871-y","DOIUrl":"https://doi.org/10.1007/s10103-026-04871-y","url":null,"abstract":"<p><p>This review summarizes recent advances in the pathogenesis of pathological scars, including hypertrophic scars and keloids, and their photodynamic therapy (PDT). The formation of pathological scars involves fibroblast dysfunction, imbalanced ECM metabolism, chronic inflammation, aberrant activation of multiple signaling pathways (e.g., TGF-β/Smad, JAK/STAT, PI3K/Akt/mTOR), epigenetic regulation, and biomechanical factors, against a background of genetic susceptibility. As a minimally invasive and targeted therapy, PDT acts through the generation of reactive oxygen species (ROS) upon light activation of photosensitizers, leading to fibroblast death, suppression of collagen synthesis, interruption of key signaling pathways, and modulation of immune responses and angiogenesis, thereby effectively improving scars. Recent developments in novel photosensitizers and nanotechnology-based delivery systems have significantly enhanced the penetration and efficacy of PDT, offering new directions for its precise clinical application.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning enhanced optical spectroscopy for breast cancer diagnosis: A review.","authors":"Mihir Nakul, Sanket Dinesh Rao, Manikanth Karnati, Farhath Aziz, Devadiga Pooja Bhaskar, Budheswar Dehury, Nirmal Mazumder","doi":"10.1007/s10103-026-04882-9","DOIUrl":"10.1007/s10103-026-04882-9","url":null,"abstract":"<p><p>This literature review examines the transformative role of machine learning (ML) and deep learning (DL) in enhancing optical spectroscopy for breast cancer diagnosis. By synthesizing advancements from peer-reviewed studies (2015-2025), we evaluate how ML/DL integration improves the detection of malignancy-associated biochemical changes, enabling noninvasive, rapid, and accurate differentiation between healthy and cancerous tissues. This review highlights key spectroscopic modalities, such as Raman, fluorescence, diffusive optical spectroscopy (DOS), and photoacoustic spectroscopy (PAS), and their integration with AI-driven models, such as convolutional neural networks (CNNs), support vector machines (SVMs), and logistic regression. These techniques achieve diagnostic accuracies of up to 94% in subtype classification (e.g., luminal A, HER2-positive) by analyzing spectral biomarkers such as hemoglobin, lipids, and collagen. Challenges such as data variability, model interpretability, and clinical integration barriers are critically assessed. These findings underscore the potential of ML/DL-enhanced spectroscopy to standardize diagnostics, reduce unnecessary biopsies, and personalize treatment monitoring. Future directions emphasize the need for explainable AI (XAI), multimodal data fusion, and large-scale, diverse datasets to bridge translational gaps. By addressing technical, ethical, and regulatory hurdles, this integration promises to advance early detection, improve clinical outcomes, and reshape precision oncology.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13149704/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of GV20-based wearable low-level laser acupoint therapy for pain on head.","authors":"Wen-Fang Lei, Arslan Sadiq, Zhen-Yang Cui, Xu-Bo Wu, Shin-Da Lee","doi":"10.1007/s10103-026-04877-6","DOIUrl":"https://doi.org/10.1007/s10103-026-04877-6","url":null,"abstract":"<p><p>The effectiveness of GV20-based Wearable Low-level Laser Acupoint Therapy for pain on right temporal head was investigated. The study was designed as a triple-blinded, randomized controlled clinical trial. The deep pressure pain targeted by 300g monofilament and Visual Analog Scale (VAS, 0-10) scores on right temporal head were assessed were randomly assigned to wearable LLLAT (650 nm, 5 mW, 30 min, about 9 joule each acupoint) on Baihui (GV20) with four Sishencong (EX-HN1) or sham group (n=24 vs 24). The mean VAS pain scores in wearable LLLAT group after GV20 with EX-HN1 acupoint treatment were significantly decreased comparing with sham group. This trial suggested that wearable Low-level Laser Acupoint Therapy 650 nm 5 mW for 30 min each acupoint simultaneously could effectively reduce deep pressure pain on temporal head.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fractional CO₂ laser treatment of post-surgical nasal scars: a retrospective analysis on 8 patients.","authors":"Emanuele Maria Cipollini, Luca Mariano, Cristiano Morini, Alessandro Clementi, Luca Guarino, Marco Gratteri, Elena Zappia, Annunziata Dattola, Steven Paul Nisticò, Giovanni Cannarozzo","doi":"10.1007/s10103-026-04827-2","DOIUrl":"https://doi.org/10.1007/s10103-026-04827-2","url":null,"abstract":"<p><p>Surgical scars of the nasal region following basal cell carcinoma (BCC) excision often present aesthetic and functional challenges, particularly the \"trap-door\" deformity related to flap reconstruction. Fractional CO₂ laser represents a promising therapeutic option to improve scar quality through collagen remodeling and dermal reorganization. This retrospective study analysed eight patients (six males and two females, mean age 71 ± 10 years) with nasal post-surgical scars after BCC excision and flap reconstruction. All patients underwent two sessions of fractional CO₂ laser in Deep Pulse or High Pulse modes, with a two-month interval between treatments. Clinical outcomes were evaluated using the Patient and Observer Scar Assessment Scale (POSAS) and three-dimensional profilometry with the Antera 3D system before treatment and two months after the last session. A statistically significant improvement was observed in both patient-reported (overall opinion p = 0.0007; thickness p = 0.03) and observer-reported parameters (vascularity p = 0.007; thickness p = 0.005; pliability p = 0.02; overall opinion p = 0.00004). Antera 3D analysis showed a mean reduction in scar depression of 23.5% (p = 0.01). All patients developed transient erythema and edema that were resolved within a week. Fractional CO₂ laser proved to be a safe and effective method to improve nasal post-surgical scars, enhancing texture, vascularity, and contour.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13134997/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preclinical evidence of photobiomodulation and clarified açaí in submandibular gland protection during antineoplastic therapy: an experimental study.","authors":"Wallacy Watson Pereira Melo, Walessa Alana Bragança Aragão, Leonardo de Oliveira Bittencourt, Luciana Eiró-Quirino, Paulo Fernando Santos Mendes, Vinicius Ruan Neves Santos, Hadassa Helez Neves Ferreira, José Messias Perdigão, Hannah Gil de Farias Morais, Herve Rogez, Roseana Almeida Freitas, Manoela Domingues Martins, Rafael Rodrigues Lima, Renata Duarte de Souza-Rodrigues","doi":"10.1007/s10103-026-04876-7","DOIUrl":"10.1007/s10103-026-04876-7","url":null,"abstract":"","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13132951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of resistance training with/without Photobiomodulation on muscle and respiratory function in difficult-to-control asthma: a randomized trial.","authors":"Ivan Peres Costa, Emilia Raposo Nascimento, Lawrence P Cahalin, Etiene Farah Teixeira Carvalho, Edvane Aparecida Braz Araujo Silva, Roberto Stirbulov, Rodolfo Paula Vieira, Simone Dal Corso, Nivaldo Antônio Parizotto, Raquel Agnelli Mesquita-Ferrari, Luciana Maria Malosá Sampaio","doi":"10.1007/s10103-026-04880-x","DOIUrl":"10.1007/s10103-026-04880-x","url":null,"abstract":"<p><p>Difficult-to-Control Asthma (DTCA) is characterized by persistent symptoms, frequent exacerbations, and reduced functional capacity despite optimized pharmacological therapy. Patients with DTCA commonly exhibit peripheral muscle dysfunction, making strategies that may potentiate the effects of resistance training clinically relevant. Photobiomodulation therapy (PBMT) has been investigated as a modality capable of improving muscle performance and exercise tolerance. The aim of this study was to determine whether combining resistance training with LED-based PBMT (RT+LEDT) results in greater improvements in peripheral muscle strength and functional exercise capacity compared with resistance training alone in adults with DTCA. This randomized, triple-blind controlled trial included 30 adults with DTCA who met predefined inclusion and exclusion criteria. Participants were allocated equally to an experimental group (RT+LEDT; n = 15) or a control group (RT; n = 15). Both groups completed supervised resistance training twice weekly for 12 weeks. The experimental group received active PBMT before each session, while the control group received placebo PBMT. The primary outcome was peripheral muscle strength assessed by one-repetition maximum (1RM). Secondary outcomes included cardiopulmonary exercise test variables, shuttle walk test performance, spirometry, physical activity level, and asthma control. Post-intervention group comparisons were performed using independent t-tests. Compared with RT alone, RT+LEDT produced greater post-treatment gains in 1RM strength across major muscle groups, along with significant improvements in oxygen consumption at the anaerobic threshold and shuttle walk test distance. No between-group differences were observed in pulmonary function or asthma control. PBMT combined with resistance training yielded clinically meaningful improvements in muscle strength and functional capacity, suggesting its potential as an adjunctive strategy in rehabilitation programs for DTCA.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13132882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen remodeling drives continence recovery: radiofrequency therapy ameliorates SUI via TGF-β1/Smad2 pathway activation - evidence from a rat model.","authors":"Liping Zhu, Yanhua Zhou, Yao Tong, Chengyu Zhou, Li Jiang, Xuhong Li","doi":"10.1007/s10103-026-04879-4","DOIUrl":"10.1007/s10103-026-04879-4","url":null,"abstract":"","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation combined with dynamic thermal imaging as a diagnostic tool for chronic leg ulcers.","authors":"Lilach Gavish, David Ben Daniel, Rita Lo, Sarah Olga Grinbaum, S David Gertz, Oshrit Hoffer, Rose Raizman","doi":"10.1007/s10103-026-04878-5","DOIUrl":"10.1007/s10103-026-04878-5","url":null,"abstract":"","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13128689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HoLEP versus ThuFLEP: perioperative and functional outcomes in prostate enucleation.","authors":"Ersin Gokmen, Turker Altuntas, Mohammad Yasir Sahak, Hamza Tunahan Midilli, Gunal Ozgur, Kamil Cam, Tufan Tarcan, Tarik Emre Sener","doi":"10.1007/s10103-026-04857-w","DOIUrl":"https://doi.org/10.1007/s10103-026-04857-w","url":null,"abstract":"","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"41 1","pages":""},"PeriodicalIF":2.4,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13106247/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}