Lasers in Medical Science最新文献

{"title":"Tissue repair after surgical debridement with diode laser (980 nm) and surgical debridement combined with photobiomodulation (808 nm) in pressure wounds in a murine model.","authors":"Ana Maria Freitas Franco Nascimento Pinto, José Mateus Santos Junior, Cecília Pedroso Turssi, Luciana Almeida-Lopes, Andresa Borges Soares, Vera Cavalcanti de Araújo, Aguinaldo Silva Garcez, Fabricio Passador-Santos","doi":"10.1007/s10103-025-04580-y","DOIUrl":"https://doi.org/10.1007/s10103-025-04580-y","url":null,"abstract":"<p><strong>Purpose: </strong>To assess the biological impact on tissue repair of diode laser debridement (980 nm) compared to surgical debridement with scissors and forceps, and surgical debridement in combination with photobiomodulation.</p><p><strong>Methods: </strong>Pressure wounds were created on the backs of male BalbC/Mus musculus mice. After hair removal, two round ferrite magnet plates (12 mm × 4 mm, 1000 gauss, 10.3 g each) were applied to compress the dorsal skin of each animal for three cycles of 12 h. The animals were divided into three groups of 12 animals: G1 - surgical debridement; G2 - surgical debridement and photobiomodulation (808 nm, 2 J, 100 mW, one central point); and G3 - diode laser debridement (980 nm, continuous mode, 1.2 W). The debridement was performed on the 5th day after injury, and euthanasia occurred on the 7th and 9th day after debridement. The injured area was monitored until day 12 or day 14, depending on the euthanasia schedule.</p><p><strong>Results: </strong>The acute inflammatory process was not statistically different in any group after seven or nine days. However, regarding the chronic inflammatory process, lower inflammation scores were observed in G2 and G3. G2 showed a more advanced repair process compared to the other groups.</p><p><strong>Conclusion: </strong>Surgical debridement associated with photobiomodulation presented the best results in reducing the inflammatory process and enhancing tissue repair. The use of the diode laser (980 nm) may be an efficient option in debriding pressure wounds and did not present significant detrimental effects when compared to the other studied debridement modalities.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"329"},"PeriodicalIF":2.4,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144742435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of high-intensity laser therapy in patients with spinal radiculopathy: a systematic review with meta-analysis.","authors":"Hernán Andrés de la Barra Ortiz, Nivaldo Antonio Parizotto, Richard Eloin Liebano","doi":"10.1007/s10103-025-04575-9","DOIUrl":"10.1007/s10103-025-04575-9","url":null,"abstract":"<p><p>Spinal radiculopathy (SRD) significantly burdens healthcare systems due to its complexity and resulting disabilities. High-Intensity Laser Therapy (HILT) is a promising treatment for managing SRD pain. The aim of this review is to assess the efficacy of HILT in the management of pain associated with SRD. A literature search across PubMed, Scopus, Web of Science, ScienceDirect, EBSCOhost, PEDro, and Google Scholar up to July 1, 2025, aimed to identify randomized clinical trials (RCTs) comparing HILT with other therapies for SRD. The primary outcome focused on pain intensity using the visual analog scale (VAS), with secondary outcomes including disability with the Neck Disability Index (NDI), Oswestry Disability Index (ODI), and range of movement (ROM). RCT quality was assessed using the Risk of Bias 2 tool (RoB2), and a meta-analysis employed the mean difference (MD) and the standardized mean difference (SMD). Evidence-based recommendations were provided with a GRADE approach. Eighteen studies were included-six on cervical and twelve on lumbar SRD-encompassing a total of 1,095 participants (555 received HILT; 540 were controls). The overall RoB was 38.9%, primarily due to shortcomings in outcome measurement and the randomization process. HILT significantly reduced pain when combined with physical therapy (MD = - 1.4 cm; 95% CI: - 2.1, - 0.7; p < 0.05) and compared to placebo (MD = - 1.8 cm; 95% CI: - 3.1, - 0.6). It also reduced disability, whether applied alone (SMD = 2.3; 95% CI: 0.3, 4.4), with physical therapy (SMD = 2.6; 95% CI: 1.0, 4.1), or with exercise (SMD = 0.9; 95% CI: 0.3, 1.5). For ROM, significant improvement was found only when HILT was combined with physical therapy (SMD = 1.1; 95% CI: 0.6, 1.6; p < 0.01). Although the evidence was considered important, its certainty was rated low to very low. HILT is more effective than placebo in reducing pain and disability in individuals with cervical or lumbar radiculopathy, particularly when combined with physical therapy or therapeutic exercise. Future RCTs should integrate therapeutic exercise and ensure greater methodological rigor, particularly through the standardization of outcome measures.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"328"},"PeriodicalIF":2.4,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel chlorine derivative ShengTaiBuFen mediated photodynamic therapy on a mouse wound model infected with methicillin-resistant Staphylococcus aureus.","authors":"Shutian Wu, Jianhua Huang, Lei Shi, Minfeng Wu, Jingjuan Shi, Hui Tao, Xiuli Wang, Shanshan Li, Hongwei Wang","doi":"10.1007/s10103-024-04249-y","DOIUrl":"10.1007/s10103-024-04249-y","url":null,"abstract":"<p><p>Methicillin-resistant Staphylococcus aureus (MRSA) is the most common and difficult-to-treat pathogen in chronic wounds. In this study, a novel derivative of chlorin, ShengTaiBuFen (STBF), was used as a photosensitiser to investigate the effect of STBF mediated photodynamic therapy (STBF-PDT) on MRSA-infected ulcers. Appropriate incubation time and concentration parameters were initially explored to evaluate the anti-MRSA performance of STBF-PDT in vitro. An infected mouse skin ulcer model was further established to assess the antibacterial ability of STBF-PDT on MRSA in ulcers and the ultimate effect on ulcers. Our data showed that the antibacterial activity of STBF-PDT against planktonic MRSA was strongest at 25 µM concentration in a light dose-dependent way, and MRSA strains were completely eliminated at 120 J/cm². The in vivo study showed the STBF-PDT group had basically healed (96.93 ± 2.21%), while the untreated group (28.83 ± 10.25%), the red light group (36.63 ± 13.08%) and the STBF group (32.95 ± 9.38%) had poor healing. STBF-PDT displayed marked bacteriostatic effect in vivo as opposed to the other three groups. The mean viable bacterial log of wounds subjected to STBF-PDT revealed 6.80 ± 6.98 log cfu/g (a substantial reduction of 99.83% versus the untreated control). STBF was believed to be an advantageous and reliable photosensitizer, and its mediated PDT provided a reliable and safe modality for the clinical treatment of potentially drug-resistant microbial infectious diseases such as chronic wounds.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"326"},"PeriodicalIF":2.4,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144718091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photodynamic therapy for the treatment of grade B periodontitis: a systematic review of randomized controlled trials.","authors":"Wei Feng, Yasir Rehman Khattak, Amir A Majeed, Zainab Shah, Uzma Saeed Hasan, Iftikhar Ahmad","doi":"10.1007/s10103-025-04576-8","DOIUrl":"10.1007/s10103-025-04576-8","url":null,"abstract":"<p><strong>Objectives: </strong>This systematic review evaluates the efficacy of photodynamic therapy (PDT) compared to conventional treatments in Grade B periodontitis.</p><p><strong>Methods: </strong>A comprehensive electronic search was conducted across PubMed, Google Scholar, Dimensions, Ebase, X-mole, and Semantic Scholar, following the PRISMA guidelines. Only randomized controlled trials (RCTs) comparing PDT with conventional periodontal treatments were included. Primary clinical outcomes included probing pocket depth (PPD), clinical attachment loss (CAL), and bleeding on probing (BOP).</p><p><strong>Results: </strong>Data from 26 RCTs involving 753 patients were analyzed, with PDT applied in 219 patients (130 males, 109 females) and conventional treatment in 314 patients (173 males, 141 females). PDT significantly reduced PPD (2.6-5.23 mm) and CAL (1.26-6.00 mm), showing comparable or superior outcomes to scaling and root planing (SRP) in several RCTs. Despite these promising results, heterogeneity in photosensitizer type, laser wavelength (660-810 nm), energy dose, and PDT session protocols contributed to variability in outcomes.</p><p><strong>Conclusion: </strong>PDT demonstrates significant clinical benefits in Grade B periodontitis treatment, particularly in PPD reduction, CAL improvement, and BOP control. However, variability in treatment protocols and limited long-term follow-up data (median: 6 months) highlight the need for standardized guidelines and further high-quality RCTs.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"327"},"PeriodicalIF":2.4,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144731971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photobiomodulation therapy in the prevention of chemotherapy-induced alopecia in breast cancer patients: a randomized controlled trial.","authors":"Marithé Claes, Jolien Robijns, Laura Lambrichts, Sofie Van Duffel, Philippe Bulens, Jeroen Mebis","doi":"10.1007/s10103-025-04577-7","DOIUrl":"10.1007/s10103-025-04577-7","url":null,"abstract":"<p><strong>Purpose: </strong>Chemotherapy-induced alopecia (CIA) is a prominent side effect of chemotherapy, negatively impacting the patient's body image and self-confidence. Scalp cooling (SC) has emerged as a preventive option for CIA, but the success rate and adherence vary. Research shows that photobiomodulation (PBM) can improve hair growth by stimulating cell proliferation and repair processes. This trial aims to evaluate the effectiveness of PBM combined with SC in preventing CIA.</p><p><strong>Methods: </strong>A randomized, controlled trial with 29 breast cancer patients undergoing taxane-based chemotherapy was performed at the Jessa Hospital (Belgium). Patients were randomized into the control (n = 16) or the intervention group (n = 13). Blinded researchers evaluated scalp coverage and hair thickness, while questionnaires were administered to assess scalp coverage, satisfaction, and health-related quality of life (HRQL). The trial was registered at clinicaltrials.gov (NCT05177289, 4th of January 2022).</p><p><strong>Results: </strong>The scalp coverage and hair thickness did not differ significantly between the two groups. Patients in the PBM group scored higher on global health (P = 0.043), physical functioning (P = 0.039), role functioning (P = 0.049), and social functioning (P = 0.038). Patients receiving a paclitaxel-based chemotherapy showed less hair loss compared to patients undergoing a docetaxel-based regimen (Ps < 0.001). No differences in SC adherence could be observed between the two groups.</p><p><strong>Conclusions: </strong>The addition of PBM to SC did not increase its efficacy in preventing hair loss during chemotherapy. Patients in the PBM group did score their HRQL higher compared to the control group. Further research is required to establish the use of PBM in CIA prevention and to corroborate these findings.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"325"},"PeriodicalIF":2.1,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does photobiomodulation before or after in-office dental bleaching influence sensitivity reduction? A randomized clinical trial.","authors":"Elma Vieira Takeuchi, Beatriz Lopes de Oliveira, Brennda Lucy Freitas de Paula, Critiane de Melo Alencar, Jesuina Lamartine Nogueira Araujo, Cecy Martins Silva","doi":"10.1007/s10103-025-04566-w","DOIUrl":"https://doi.org/10.1007/s10103-025-04566-w","url":null,"abstract":"","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"324"},"PeriodicalIF":2.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosting osteoblast differentiation: enhancing the effects of low-level blue laser therapy on human embryonic stem cell-derived mesenchymal stem cells to improve viability and calcium deposition.","authors":"Khalid M AlGhamdi, Ashok Kumar, Musaad Alfayez, Amer Mahmood","doi":"10.1007/s10103-025-04564-y","DOIUrl":"10.1007/s10103-025-04564-y","url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs) are widely studied for their regenerative capacities in bone tissue repair. Low-level laser therapy (LLLT) has emerged as a promising method to stimulate stem cell proliferation, viability, and differentiation. In this study, we focus on how low-level blue-laser treatment (457 nm) affects human embryonic stem cell-derived mesenchymal stem cells (hESC-MSCs) at various energy densities, highlighting its potential to enhance osteogenic differentiation for clinical applications in treating osteoporosis. To determine how low-level blue-laser treatment at various energy densities (0.5-5.0 J/cm²) influences the proliferation, viability, migration, and osteogenic differentiation of hESC-MSCs. hESC-MSCs were cultured to near confluence, then irradiated at doses ranging from 0.5 to 5.0 J/cm². Cell proliferation, viability, and migration were assessed at 72 h. Flow cytometry evaluated CD146 expression, and Alkaline phosphate (ALP) activity was measured. Osteogenic gene expression (Runx2, ALP, BMP2, BMP4, and osteonectin) and in vitro mineralization were also examined. Blue-laser treatment at 0.5-3.5 J/cm² significantly increased cell proliferation (p < 0.01) and viability (p < 0.05), while migration was enhanced at 0.5-2.5 J/cm² (p < 0.001). CD146 expression rose at 0.5, 1.0, and 2.0 J/cm², with a 1.9-fold increase in ALP activity at 2.0 J/cm². Osteogenic markers and mineralization were likewise upregulated at 2.0 J/cm², indicating enhanced osteoblast differentiation. These findings indicate that LLLT combined with a blue laser results in changes in various biological processes at the cellular and genetic levels in hESC-MSCs, indicating that these cells are sensitive to blue laser treatment. Thus, these results demonstrate that hMSCs are responsive to blue-laser treatment, which may be used in the clinic to treat osteoporosis.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"323"},"PeriodicalIF":2.4,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12283813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of antimicrobial photodynamic therapy for bacterial reduction in endodontics of primary teeth: an in vitro study.","authors":"Maria Luiza Costabeber Perin, Maysa Lannes Duarte, Kenderson Santos Silva, Gloria Fernanda Castro, Antônio Ferreira-Pereira, Maristela Barbosa Portela, Laura Guimarães Primo","doi":"10.1007/s10103-025-04570-0","DOIUrl":"https://doi.org/10.1007/s10103-025-04570-0","url":null,"abstract":"<p><p>Different adjunct methods have been proposed to reduce microorganisms during endodontic treatment. This study aimed to evaluate microbial reduction in vitro in the root canals of extracted primary teeth contaminated with Enterococcus faecalis (ATCC), following antimicrobial photodynamic therapy (aPDT). Twenty-one root canals of primary teeth, decals from the repository of a research group were contaminated and divided into three groups (7 roots per group): the control group (CG) underwent chemical-mechanical preparation (CMP) with mechanized instrumentation and smear layer removal; intervention group 1 (G1) underwent aPDT without CMP; and intervention group 2 (G2) underwent CMP followed by aPDT. Significant bacterial reductions were observed across all the groups (p = 0.001), with final mean bacterial loads statistically lower than the initial mean levels (p < 0.05, Mann-Whitney test). The G2 group demonstrated the highest mean microbial reduction, with 5 roots achieving complete bacterial elimination (100% reduction). Comparatively, the mean colony-forming unit (CFU) reduction values in G2 were comparable to those in CG (p = 0.318), and both were significantly higher than in G1 (p = 0.001 for CG vs. G1; p = 0.001 for G2 vs. G1). Under the conditions tested, aPDT served as an effective adjunct technique to pulpectomy, but did not outperform conventional pulpectomy performed alone.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"322"},"PeriodicalIF":2.1,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review via text mining approaches of human and veterinary applications of photobiomodulation: focus on multiwave locked system laser therapy.","authors":"Annalisa Previti, Michela Pugliese, Silvia Meggiolaro, Annamaria Passantino","doi":"10.1007/s10103-025-04572-y","DOIUrl":"10.1007/s10103-025-04572-y","url":null,"abstract":"<p><p>To evaluate scientific literature regarding the application of Photobiomodulation (PBM), with a special focus on Multiwave Locked System (MLS^®) laser therapy, an innovative machine learning method was used. PBM therapy has occurred as a non-invasive therapeutic approach in human and veterinary medicine. It is widely used to treat various musculoskeletal conditions, promote wound healing, and alleviate inflammation and pain through cellular modulation mechanisms.The methods used were text mining (TM) and topic analysis (TA) to store and process large datasets in digital formats. The investigation followed a systematic approach based on the PRISMA guidelines. Our analysis of 211 selected studies, conducted from 1990 to 2024, identified five key topics in PBM research: therapeutic uses, cellular effects and mechanisms of action, pain control, outcomes in oral pathologies, and laser biostimulation of wounds and bones. Notably, research highlights the effectiveness of MLS^® in musculoskeletal pain management, neuropathic conditions, and complex wound care, both in human and veterinary medicine. Despite increasing evidence supporting its clinical applications, significant gaps remain regarding standardized treatment protocols and a deeper understanding of molecular mechanisms.Further research is necessary to elucidate optimal parameters for different clinical applications, with particular emphasis on individualized dosing, frequency, and treatment duration, to maximize therapeutic outcomes.</p>","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"321"},"PeriodicalIF":2.1,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12274228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144659568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing mathematical skills in children with dyscalculia: the effects of transcranial photobiomodulation.","authors":"Leyla Rastgar-Farajzadeh, Mohammad Ali Nazari, Karin Kucian, Tohid Jafari-Koshki, Maryam Moghadam Salimi, Abbas Ebrahimi-Kalan","doi":"10.1007/s10103-025-04561-1","DOIUrl":"https://doi.org/10.1007/s10103-025-04561-1","url":null,"abstract":"","PeriodicalId":17978,"journal":{"name":"Lasers in Medical Science","volume":"40 1","pages":"319"},"PeriodicalIF":2.1,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}