Photobiomodulation with LED does not alter antibiotic sensitivity in ATCC and burn-patient bacteria.

IF 2.4 4区医学 Q3 ENGINEERING, BIOMEDICAL

Lasers in Medical Science Pub Date : 2025-09-23 DOI:10.1007/s10103-025-04642-1

Flávia Fernanda de Oliveira Assunção, Lucas Chaves, Érika Nascimento, Alessandro Marcio Hakme Silva, Thiago Domingues Stocco, Vitor Hugo Balasco Serrão, Roberto Martines, Elaine Caldeira de Oliveira Guirro, Alceu Afonso Jordão Júnior, Rinaldo Roberto de Jesus Guirro

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引用次数: 0

Abstract

Introduction: The association of photobiomodulation (PBM) and antibiotics has been little investigated for bacterial control.

Objectives: This study aimed to verify possible changes in the antibiotic sensitivity profile in ATCC-type bacteria and bacteria collected from burned patients after irradiation with 465 and 630 nm LED at 30, 40 and 50 J/cm².

Methods: The species Staphylococcus aureus (ATCC^®BAA-977), Pseudomonas aeruginosa (ATCC 27853), and Staphylococcus aureus and Pseudomonas aeruginosa from burned patients were used as proof of concept. A qualitative method was adopted to verify the antibiotic sensitivity profile. To quantify oxidative stress, assessments were performed using Malondialdehyde (MDA) and antioxidant action using glutathione (GSH). Data were analyzed by Shapiro-Wilk and ANOVA single-factor tests, with Tukey post-hoc (p < 0.05).

Results: Differences between the irradiated groups were found, however, without statistical significance or representing a change in the sensitivity profile of the bacteria investigated. The highest production of oxidative stress was at 465 nm (30 J/cm²), and no glutathione production was detected.

Conclusion: LED irradiation was found to be safe for application to bacterial colonies, as it did not modify their antibiotic susceptibility profile, despite inducing oxidative stress.

查看原文本刊更多论文

LED光生物调节不会改变ATCC和烧伤患者细菌的抗生素敏感性。

导读：光生物调节（PBM）与抗生素之间的关系在细菌控制方面的研究很少。目的：本研究旨在验证465和630 nm LED以30、40和50 J/cm2照射烧伤患者后atcc型细菌和细菌的抗生素敏感性谱可能发生的变化。方法：以烧伤患者的金黄色葡萄球菌（ATCC®BAA-977）、铜绿假单胞菌（ATCC 27853）、金黄色葡萄球菌和铜绿假单胞菌作为概念验证。采用定性方法验证抗生素敏感性谱。为了量化氧化应激，使用丙二醛（MDA）进行评估，使用谷胱甘肽（GSH）进行抗氧化作用。通过Shapiro-Wilk和ANOVA单因素检验分析数据，并使用Tukey事后检验(p)结果：发现辐照组之间存在差异，但没有统计学意义或表明所调查细菌的敏感性谱发生变化。氧化应激在465 nm （30 J/cm2）处产生最高，未检测到谷胱甘肽的产生。结论：LED照射对细菌菌落是安全的，虽然会引起氧化应激，但不会改变细菌的抗生素敏感性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Lasers in Medical Science 医学-工程：生物医学

CiteScore

4.50

自引率

4.80%

发文量

192

审稿时长

3-8 weeks

期刊介绍： Lasers in Medical Science (LIMS) has established itself as the leading international journal in the rapidly expanding field of medical and dental applications of lasers and light. It provides a forum for the publication of papers on the technical, experimental, and clinical aspects of the use of medical lasers, including lasers in surgery, endoscopy, angioplasty, hyperthermia of tumors, and photodynamic therapy. In addition to medical laser applications, LIMS presents high-quality manuscripts on a wide range of dental topics, including aesthetic dentistry, endodontics, orthodontics, and prosthodontics. The journal publishes articles on the medical and dental applications of novel laser technologies, light delivery systems, sensors to monitor laser effects, basic laser-tissue interactions, and the modeling of laser-tissue interactions. Beyond laser applications, LIMS features articles relating to the use of non-laser light-tissue interactions.