Journal of the Science of Food and Agriculture最新文献

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A novel β-galactosidase from anaerobic thermophilic Caldicellulosiruptor saccharolyticus DSM 8903 with high potential for lactulose production. 一种新的β-半乳糖苷酶,来自厌氧嗜热钙纤维素裂解糖解菌DSM 8903,具有高产乳果糖的潜力。
IF 3.3 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-05-21 DOI: 10.1002/jsfa.14389
Sirilak Baramee, Ake-Kavitch Siriatcharanon, Rattiya Waeonukul, Patthra Pason, Chakrit Tachaapaikoon, Khanok Ratanakhanokchai
{"title":"A novel β-galactosidase from anaerobic thermophilic Caldicellulosiruptor saccharolyticus DSM 8903 with high potential for lactulose production.","authors":"Sirilak Baramee, Ake-Kavitch Siriatcharanon, Rattiya Waeonukul, Patthra Pason, Chakrit Tachaapaikoon, Khanok Ratanakhanokchai","doi":"10.1002/jsfa.14389","DOIUrl":"https://doi.org/10.1002/jsfa.14389","url":null,"abstract":"<p><strong>Background: </strong>Lactulose is a rare disaccharide with applications in the pharmaceutical, food, and animal feed industries. It can be synthesized through a transgalactosylation reaction between fructose and lactose, mediated by β-galactosidases. However, the need for high excess fructose to drive lactulose synthesis, along with relatively low yields and selectivity, has posed challenges for commercial production. This study extensively characterizes a novel lactulose-producing glycoside hydrolase family 2 (GH2) β-galactosidase from Caldicellulosiruptor saccharolyticus expressed in Escherichia coli (CsBgl2A).</p><p><strong>Results: </strong>The enzyme exhibited high activity across a broad pH and temperature range, maintaining more than 95% of its maximal activity between pH 6.0-9.0 and 40-55 °C. Under optimal conditions - 750 g L<sup>-1</sup> initial sugar concentration, a fructose-to-lactose molar ratio of 4, 6 μmol L<sup>-1</sup> enzyme, pH 7.0, and 55 °C - the reaction produced a maximum lactulose concentration of 81.9 g L<sup>-1</sup> at 3 h, with 32.5% lactulose yield, 10.9% lactulose-to-total sugars yield, and 61.6% lactose conversion. By 6 h, nearly all lactose had been hydrolyzed.</p><p><strong>Conclusion: </strong>CsBgl2A is among the few β-galactosidases that achieve high lactulose yields with low fructose-to-lactose ratios. This enzyme is thus promising for producing low lactose dairy products and for cost-effective lactulose synthesis. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lactobacillus plantarum with lignin prevents Helicobacter pylori mediated DSS-colitis through improved intestinal integrity and reducing colonic inflammation in C57BL/6J mice. 植物乳杆菌与木质素通过改善C57BL/6J小鼠肠道完整性和减少结肠炎症来预防幽门螺杆菌介导的dss -结肠炎。
IF 3.3 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-05-21 DOI: 10.1002/jsfa.14374
Venugopal Kaliyamoorthy, Justin Packia Jacop, Hairul Islam Mohamed Ibrahim, Sivakumar Kandhasamy
{"title":"Lactobacillus plantarum with lignin prevents Helicobacter pylori mediated DSS-colitis through improved intestinal integrity and reducing colonic inflammation in C57BL/6J mice.","authors":"Venugopal Kaliyamoorthy, Justin Packia Jacop, Hairul Islam Mohamed Ibrahim, Sivakumar Kandhasamy","doi":"10.1002/jsfa.14374","DOIUrl":"https://doi.org/10.1002/jsfa.14374","url":null,"abstract":"<p><strong>Background: </strong>Probiotics are beneficial microorganisms that affect stomach acid and reduce bile production in the host organism. Helicobacter pylori infection causes chronic gastritis, peptic ulcer, gastric carcinoma and gastric lymphoma. Probiotic mediated gut health improvement in H. pylori infection remains underestimated.</p><p><strong>Objective: </strong>The study aimed to develop Venu Lactiplantibacillus plantarum (VLP) surface coated with lignin and examine their protective effects and metabolic pathways in a colitis model using dextran sulphate sodium (DSS) in male C57BL/6J mice.</p><p><strong>Materials and methods: </strong>The toxic effects of dietary lignin were assessed on H. pylori and L. plantarum (VLP) using bacterial growth optical density (OD) at 600 nm. C57BL/6J male mice were induced with DSS colitis with H. pylori antigens and VLP with lignin was used to reduce the gut colitis by examined the length of the colon, the presence of inflammation, and the level of oxidative stress in mice. The collapse of the intestinal barrier was further identified by investigating gut permeability and inflammatory markers such as tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interferon-γ (IFN-γ), and transforming growth factor-β (TGF-β) using enzyme linked immunosorbent assay (ELISA) platform methods.</p><p><strong>Result: </strong>The toxic effects of dietary lignin were assessed and it was found that lignin treatment exhibited substantial inhibition against H. pylori inhibition at a dose of 5 μmol/L and the OD value at 600 nm. The viability of VLPs treated with lignin exhibited a 2.0 CFU/mL with significant increase in viable growth after a period of 3 h. The findings demonstrated that the application of L. plantarum with lignin treatment effectively decreased the levels of inflammatory cytokines in colon tissues affected by inflammation. The ulcerative colitis disease activity index was greatly lowered by modulating the immunological response, boosting antioxidant capacity, and regulating inflammatory signalling pathways. In vitro experiments demonstrated that VLP-treated Caco-2 cells effectively decreased H. pylori infection and enhanced their survival ability, invasion, and adhesion.</p><p><strong>Conclusion: </strong>To summarize, the presence of lignin in VLP has the ability to alleviate colitis generated by DSS and H. pylori antigens, and its therapeutic capabilities against ulcerative colitis in a mouse model have been investigated. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Quercetin-based treatment improves eggshell quality in aged laying hens by modulating immune response, eggshell gland health and serum metabolome. 槲皮素处理通过调节免疫应答、蛋壳腺健康和血清代谢组改善老龄蛋鸡蛋壳品质。
IF 3.3 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-05-19 DOI: 10.1002/jsfa.14359
Yi Wei, Yongfa Liu, Jiaqi Lei, Qiuyu Jiang, Xiangfei Geng, Yuming Guo, Bingkun Zhang
{"title":"Quercetin-based treatment improves eggshell quality in aged laying hens by modulating immune response, eggshell gland health and serum metabolome.","authors":"Yi Wei, Yongfa Liu, Jiaqi Lei, Qiuyu Jiang, Xiangfei Geng, Yuming Guo, Bingkun Zhang","doi":"10.1002/jsfa.14359","DOIUrl":"https://doi.org/10.1002/jsfa.14359","url":null,"abstract":"<p><strong>Background: </strong>Eggshell quality is a critical determinant of egg product value and consumer preference. Deficient eggshells increase breakage susceptibility, leading to economic losses and reduced marketability. Dietary interventions hold promise for improving eggshell quality. Quercetin, a naturally occurring flavonoid, exhibits diverse biological activities relevant to eggshell formation. These include antioxidant and anti-inflammatory properties, and potential modulation of estrogen levels, which are known to influence eggshell quality.</p><p><strong>Results: </strong>A total of 160 46-week-old laying hens were randomly divided into two groups, with 8 replicates in each group and 10 chickens in each replicate. The control group was fed a corn-soybean meal basal diet, while the experimental group received a basal diet supplemented with 300 mg kg<sup>-1</sup> quercetin. The trial period lasted for 10 weeks. The study showed that dietary supplementation with quercetin significantly increased eggshell thickness (P < 0.001). Eggshell ultrastructure analysis revealed significant increases in effective layer thickness (P < 0.001) and a marked decrease in the thickness ratio of the mammillary layer to total eggshell thickness (P < 0.001) with quercetin supplementation. Serum analysis indicated significantly higher levels of immunoglobulin A, immunoglobulin G, estrogen receptors and carbonic anhydrase-2 in hens supplemented with quercetin compared to controls (P < 0.05). Furthermore, quercetin supplementation reduced tubular gland edema and improved villus height, luminal epithelial cell height and gland duct diameter (P < 0.05). Quantitative real-time PCR analysis showed that quercetin supplementation altered immune responses in the liver, spleen and oviduct. Notably, quercetin supplementation increased metabolites associated with glycine, serine and threonine metabolism. These amino acids, key precursors of eggshell matrix proteins, showed increased metabolism, suggesting a potential mechanism by which quercetin supports eggshell biomineralization.</p><p><strong>Conclusion: </strong>This study suggests that compromised eggshell quality might be associated with impaired biomineralization function in the eggshell gland, potentially due to tissue damage. Conversely, dietary quercetin supplementation significantly increased eggshell thickness. We propose a potential mechanism involving the activation of glycine, serine and threonine metabolic pathways, which might enhance the biomineralization capacity and overall health of the eggshell gland. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative effect of sialic acid and 3'-sialyllactose on fecal microbiota fermentation and prebiotic activity in ETEC-challenged IPEC-J2 cells. 唾液酸和3′-唾液乳糖对ecc激菌IPEC-J2细胞粪便菌群发酵和益生元活性的影响
IF 3.3 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-05-19 DOI: 10.1002/jsfa.14372
Qiwen Zhou, Shuhuan Lu, Kejue Feng, Kai Na, Li Xiang, Min Xiao, Shuang Lu, Li Zhang, Xiangyu Li, Xiaohua Guo
{"title":"Comparative effect of sialic acid and 3'-sialyllactose on fecal microbiota fermentation and prebiotic activity in ETEC-challenged IPEC-J2 cells.","authors":"Qiwen Zhou, Shuhuan Lu, Kejue Feng, Kai Na, Li Xiang, Min Xiao, Shuang Lu, Li Zhang, Xiangyu Li, Xiaohua Guo","doi":"10.1002/jsfa.14372","DOIUrl":"https://doi.org/10.1002/jsfa.14372","url":null,"abstract":"<p><strong>Background: </strong>Sialic acid (SA) and 3'-sialyllactose (3'-SL) are potential food supplements, capable of promoting gut health and brain development. Structurally, 3'-SL can be formed by coupling a sialyl moiety to lactose via α-2,3 glycosidic linkages. However, there is currently no conclusive evidence regarding the role of the sialyl moiety in the prebiotic function of 3'-SL or its contribution to the biological activity of 3'-SL itself.</p><p><strong>Results: </strong>This study demonstrated that SA and 3'-SL differentially modulate the gut microbial community structure during in vitro fermentation, selectively promoting the growth of specific microbiota and altering short-chain fatty acid production profiles. Specifically, SA significantly increased acetate levels whereas 3'-SL mainly elevated the production of butyrate. At the cellular level, both compounds consistently strengthened intestinal barrier integrity in IPEC-J2 cells, reduced apoptosis, and alleviated oxidative stress induced by enterotoxigenic Escherichia coli.</p><p><strong>Conclusion: </strong>This study highlights the prebiotic potential of SA and 3'-SL in maintaining intestinal homeostasis, an effect likely mediated by their distinct structural properties. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Brackish water stabilizes textural quality by regulating muscle protein properties of Eriocheir sinensis during temporary fattening. 微咸水通过调节中华绒螯蟹在临时育肥过程中的肌肉蛋白特性来稳定其质地质量。
IF 3.3 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-05-19 DOI: 10.1002/jsfa.14379
Wenqing Zhai, Renyue Zhang, Siqi Zhou, Fengyi Qiao, Xugan Wu, Xichang Wang, Long Zhang
{"title":"Brackish water stabilizes textural quality by regulating muscle protein properties of Eriocheir sinensis during temporary fattening.","authors":"Wenqing Zhai, Renyue Zhang, Siqi Zhou, Fengyi Qiao, Xugan Wu, Xichang Wang, Long Zhang","doi":"10.1002/jsfa.14379","DOIUrl":"https://doi.org/10.1002/jsfa.14379","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to investigate the effects of brackish-water temporary rearing on the textural quality of raw muscle and cooked meat of the Chinese mitten crab, Eriocheir sinensis.</p><p><strong>Results: </strong>Brackish-water stabilized the textural quality of raw muscle (82.22 ± 2.19 mm min<sup>-1</sup>) and suppressed the increase in tenderness of cooked meat (51.07 ± 3.80 mm min<sup>-1</sup>) during 2 weeks of temporary rearing. The morphology of muscle bundles has a greater influence on tenderness than the morphology of thick microfilaments. Phosphoproteomic analysis showed that the differentially phosphorylated proteins were predominantly glycolytic enzymes, myofibrillar proteins, and cytoskeletal proteins, which explains the differences in raw muscle tenderness. Phosphoglucose convertase-1, pyruvate kinase, glycogen phosphorylase, myosin heavy chain, and paramyosin may serve as potential markers for assessing the textural quality of raw muscle.</p><p><strong>Conclusion: </strong>Brackish-water temporary rearing for 2 weeks stabilized textural fluctuations in raw muscle and cooked meat by regulating muscle protein properties of E. sinensis. This study provides a theoretical reference for the improvement of the texture of freshwater aquaculture species. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrasound combined with alternating anaerobic and aerobic treatment elicited gamma-aminobutyric acid accumulation in mulberry leaves. 超声联合厌氧与好氧交替处理诱导桑叶γ -氨基丁酸积累。
IF 3.3 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-05-15 DOI: 10.1002/jsfa.14373
Wenwen Xie, Yu Sun, Haile Ma, Xiumin Chen
{"title":"Ultrasound combined with alternating anaerobic and aerobic treatment elicited gamma-aminobutyric acid accumulation in mulberry leaves.","authors":"Wenwen Xie, Yu Sun, Haile Ma, Xiumin Chen","doi":"10.1002/jsfa.14373","DOIUrl":"https://doi.org/10.1002/jsfa.14373","url":null,"abstract":"<p><strong>Background: </strong>Gamma-aminobutyric acid (GABA) is a natural, non-protein amino acid with numerous health benefits. However, GABA content in plants is typically low, limiting its biological effectiveness. This study aimed to enhance GABA accumulation in mulberry leaves using ultrasonication combined with alternating anaerobic-aerobic treatment and to explore the underlying mechanisms involved.</p><p><strong>Results: </strong>A definitive screening design identified ultrasonic time, temperature, incubation duration, and anaerobic-aerobic treatment as the key factors influencing GABA accumulation. Central composite design optimization determined the optimal conditions as: ultrasonic frequency 20/35 kHz, ultrasonic time 7.3 min, temperature 42 °C, pH 7.2, power density 12 W L<sup>-1</sup>, and three cycles of 2 h anaerobic followed by 1 h aerobic treatment. Under these conditions, GABA content in treated mulberry leaves (UAML) increased 7.2-fold compared to the untreated control (CML). UAML also exhibited significant enhancements in total phenolic content (81.87%), 2,2'-azidinebis-3-ethylbenzothiazolin-6-sulfonic acid radical scavenging capacity (60.45%), 2,2-diphenyl-1-picrylhydrazyl scavenging capacity (154.09%), and angiotensin-converting enzyme inhibitory activity (71.76%). High-performance liquid chromatography (HPLC) analysis demonstrated significantly higher levels of 3-O-caffeoylquinic acid and rutin (P < 0.05) levels in UAML. Electrical conductivity of UAML was 8.22% higher than that of CML. Scanning electron microscopy visualized the increased wrinkles and open stomata on the surface of UAML, while fluorescent microscopy revealed elevated intracellular calcium ion (Ca<sup>2+</sup>) concentration in UAML.</p><p><strong>Conclusion: </strong>The combination of ultrasound and alternating anaerobic-aerobic treatment effectively enhanced GABA accumulation in mulberry leaves, providing a practical approach to producing value-added mulberry leaf products. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Peptides of corn oligopeptides improve Aβ1-42-injured SHSY5Y cells. 玉米寡肽肽可改善a β1-42损伤的SHSY5Y细胞。
IF 3.3 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-05-15 DOI: 10.1002/jsfa.14307
Hanshuo Wu, Rui Liu, Xinxue Zhang, Hualei Wang, Ganlu Meng, Jie Ren, Wenying Liu, Shuguo Li
{"title":"Peptides of corn oligopeptides improve Aβ<sub>1-42</sub>-injured SHSY5Y cells.","authors":"Hanshuo Wu, Rui Liu, Xinxue Zhang, Hualei Wang, Ganlu Meng, Jie Ren, Wenying Liu, Shuguo Li","doi":"10.1002/jsfa.14307","DOIUrl":"https://doi.org/10.1002/jsfa.14307","url":null,"abstract":"<p><strong>Background: </strong>There are more and more Alzheimer's patients. The formation of plaques of Aβ<sub>1-42</sub> in the brain is one of the main causes of Alzheimer's disease. Corn oligopeptides have natural antioxidant effects. It is aimed to develop functional corn oligopeptides to prevent Alzheimer's disease through antioxidation.</p><p><strong>Methods: </strong>According to previous laboratory studies, peptides of corn oligopeptides were screened by biological activity score and ADMET prediction, and molecular docking technology was used to screen the peptides that had high binding energy with Aβ<sub>1-42</sub>. The protective effects of the selected peptides were evaluated against oxidative stress in Aβ<sub>1-42</sub>-injured SHSY5Y cells, and the mechanism of the effects in the protein kinase A (PKA)/cAMP-response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF)-mediated signaling pathway was investigated, which is closely related to neurodegenerative and neurological diseases.</p><p><strong>Results: </strong>The results showed that three peptides (GL, FA and FQ) significantly increased the cell viability of Aβ<sub>1-42</sub>-induced cells and mitochondrial intensity and decreased extracellular lactate dehydrogenase content. They also improved intracellular oxidative stress caused by Aβ<sub>1-42</sub>, including reducing the overproduction of intracellular reactive oxygen species, and increasing the content of lipid oxidation, superoxide dismutase and glutathione peroxidase. In addition, western blot showed that treatment with GL, FA and FQ significantly increased the expression of PKA, CREB and BDNF, whereas cells injured with Aβ<sub>1-42</sub> decreased the expression of these signaling proteins.</p><p><strong>Conclusion: </strong>These results suggest that peptides of corn oligopeptides can effectively improve Aβ<sub>1-42</sub>-induced Alzheimer's disease, and may improve oxidative stress response to protective nerve cells by up-regulating the protein expression of the PKA/CREB/BDNF signaling pathway. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Microbial transformations of bioactives in fermented foods 发酵食品中生物活性物质的微生物转化
IF 3.3 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-05-15 DOI: 10.1002/jsfa.14284
Apramita Devi, H.P. Vasantha Rupasinghe, Amit Kumar Rai
{"title":"Microbial transformations of bioactives in fermented foods","authors":"Apramita Devi,&nbsp;H.P. Vasantha Rupasinghe,&nbsp;Amit Kumar Rai","doi":"10.1002/jsfa.14284","DOIUrl":"https://doi.org/10.1002/jsfa.14284","url":null,"abstract":"","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":"105 8","pages":"4057"},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to 'Organic zinc and manganese enhance eggshell ultrastructure by influencing its calcium deposition to improve eggshell quality in laying hens'. 修正了“有机锌、锰通过影响其钙沉积增强蛋壳超微结构,改善蛋鸡蛋壳品质”。
IF 3.3 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-05-15 DOI: 10.1002/jsfa.14350
{"title":"Correction to 'Organic zinc and manganese enhance eggshell ultrastructure by influencing its calcium deposition to improve eggshell quality in laying hens'.","authors":"","doi":"10.1002/jsfa.14350","DOIUrl":"https://doi.org/10.1002/jsfa.14350","url":null,"abstract":"","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the potential of spore surface-displayed keratinase for feather waste degradation using high-throughput screening. 利用高通量筛选探索孢子表面显示角化酶用于羽毛废物降解的潜力。
IF 3.3 2区 农林科学
Journal of the Science of Food and Agriculture Pub Date : 2025-05-13 DOI: 10.1002/jsfa.14361
Mingchen Yan, Zhen Wang, Huimin Zhou, Yanzhen Chen, Muhammad Saeed, Yudan Xu, Ying Chen, Zhong Ni, Zhen Fang, Huayou Chen
{"title":"Exploring the potential of spore surface-displayed keratinase for feather waste degradation using high-throughput screening.","authors":"Mingchen Yan, Zhen Wang, Huimin Zhou, Yanzhen Chen, Muhammad Saeed, Yudan Xu, Ying Chen, Zhong Ni, Zhen Fang, Huayou Chen","doi":"10.1002/jsfa.14361","DOIUrl":"https://doi.org/10.1002/jsfa.14361","url":null,"abstract":"<p><strong>Background: </strong>With the development of the global poultry industry, millions of tons of feathers are produced each year. A significant proportion is improperly discarded, leading to a substantial loss of keratin resources from feathers. Utilizing feathers in the feed industry could reduce costs substantially. However, the new fermented feed industry lacks acid-resistant keratinase, making it unable to convert feather keratin effectively in a lactic acid bacteria fermentation environment.</p><p><strong>Results: </strong>This study established a high-throughput screening method using spore surface-display to identify acid-resistant mutants generated by error-prone PCR. A mutant H4 (N43K/N143I) was obtained with a significant increase in activity under acidic conditions, and it was 1.34-fold more active than the wild type in a pH 5 environment. Bioinformatic analysis showed that the increase in stress resistance and activity of H4 was due to an increase in certain weak interaction forces and alterations in energy levels. The addition of H4 spore suspension as an enzyme preparation to feather meal fermentation feed with the participation of lactic acid bacteria improved the content of small peptides and free amino acids significantly. After being subjected to short-term high-temperature treatments, the H4 spore preparation was still able to degrade chicken feathers.</p><p><strong>Conclusion: </strong>These results suggest that the spore surface-displayed keratinase mutant H4 has potential for improved effectiveness in feather degradation within the fermented feed industry. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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