Exploring the potential of spore surface-displayed keratinase for feather waste degradation using high-throughput screening.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-05-13 DOI:10.1002/jsfa.14361

Mingchen Yan, Zhen Wang, Huimin Zhou, Yanzhen Chen, Muhammad Saeed, Yudan Xu, Ying Chen, Zhong Ni, Zhen Fang, Huayou Chen

{"title":"Exploring the potential of spore surface-displayed keratinase for feather waste degradation using high-throughput screening.","authors":"Mingchen Yan, Zhen Wang, Huimin Zhou, Yanzhen Chen, Muhammad Saeed, Yudan Xu, Ying Chen, Zhong Ni, Zhen Fang, Huayou Chen","doi":"10.1002/jsfa.14361","DOIUrl":null,"url":null,"abstract":"Background: With the development of the global poultry industry, millions of tons of feathers are produced each year. A significant proportion is improperly discarded, leading to a substantial loss of keratin resources from feathers. Utilizing feathers in the feed industry could reduce costs substantially. However, the new fermented feed industry lacks acid-resistant keratinase, making it unable to convert feather keratin effectively in a lactic acid bacteria fermentation environment.Results: This study established a high-throughput screening method using spore surface-display to identify acid-resistant mutants generated by error-prone PCR. A mutant H4 (N43K/N143I) was obtained with a significant increase in activity under acidic conditions, and it was 1.34-fold more active than the wild type in a pH 5 environment. Bioinformatic analysis showed that the increase in stress resistance and activity of H4 was due to an increase in certain weak interaction forces and alterations in energy levels. The addition of H4 spore suspension as an enzyme preparation to feather meal fermentation feed with the participation of lactic acid bacteria improved the content of small peptides and free amino acids significantly. After being subjected to short-term high-temperature treatments, the H4 spore preparation was still able to degrade chicken feathers.Conclusion: These results suggest that the spore surface-displayed keratinase mutant H4 has potential for improved effectiveness in feather degradation within the fermented feed industry. © 2025 Society of Chemical Industry.","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Science of Food and Agriculture","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/jsfa.14361","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: With the development of the global poultry industry, millions of tons of feathers are produced each year. A significant proportion is improperly discarded, leading to a substantial loss of keratin resources from feathers. Utilizing feathers in the feed industry could reduce costs substantially. However, the new fermented feed industry lacks acid-resistant keratinase, making it unable to convert feather keratin effectively in a lactic acid bacteria fermentation environment.

Results: This study established a high-throughput screening method using spore surface-display to identify acid-resistant mutants generated by error-prone PCR. A mutant H4 (N43K/N143I) was obtained with a significant increase in activity under acidic conditions, and it was 1.34-fold more active than the wild type in a pH 5 environment. Bioinformatic analysis showed that the increase in stress resistance and activity of H4 was due to an increase in certain weak interaction forces and alterations in energy levels. The addition of H4 spore suspension as an enzyme preparation to feather meal fermentation feed with the participation of lactic acid bacteria improved the content of small peptides and free amino acids significantly. After being subjected to short-term high-temperature treatments, the H4 spore preparation was still able to degrade chicken feathers.

Conclusion: These results suggest that the spore surface-displayed keratinase mutant H4 has potential for improved effectiveness in feather degradation within the fermented feed industry. © 2025 Society of Chemical Industry.

查看原文本刊更多论文

利用高通量筛选探索孢子表面显示角化酶用于羽毛废物降解的潜力。

背景：随着全球家禽业的发展，每年生产数百万吨羽毛。很大一部分被不恰当地丢弃，导致羽毛中的角蛋白资源大量流失。在饲料工业中利用羽毛可以大大降低成本。然而，新型发酵饲料行业缺乏耐酸角蛋白酶，无法在乳酸菌发酵环境下有效转化羽毛角蛋白。结果：本研究建立了一种利用孢子表面展示技术对易出错PCR产生的耐酸突变体进行高通量筛选的方法。突变体H4 （N43K/N143I）在酸性条件下活性显著增加，在pH 5环境下活性是野生型的1.34倍。生物信息学分析表明，H4抗逆性和活性的增加是由于某些弱相互作用力的增加和能级的改变。在乳酸菌参与的羽毛粉发酵饲料中添加H4孢子悬浮液作为酶制剂，显著提高了小肽和游离氨基酸的含量。H4孢子制备经过短期高温处理后，仍能降解鸡毛。结论：这些结果表明，孢子表面显示的角化酶突变体H4在发酵饲料工业中具有提高羽毛降解效率的潜力。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.