Journal of the Science of Food and Agriculture最新文献

{"title":"Ultrasound combined with alternating anaerobic and aerobic treatment elicited gamma-aminobutyric acid accumulation in mulberry leaves.","authors":"Wenwen Xie, Yu Sun, Haile Ma, Xiumin Chen","doi":"10.1002/jsfa.14373","DOIUrl":"https://doi.org/10.1002/jsfa.14373","url":null,"abstract":"<p><strong>Background: </strong>Gamma-aminobutyric acid (GABA) is a natural, non-protein amino acid with numerous health benefits. However, GABA content in plants is typically low, limiting its biological effectiveness. This study aimed to enhance GABA accumulation in mulberry leaves using ultrasonication combined with alternating anaerobic-aerobic treatment and to explore the underlying mechanisms involved.</p><p><strong>Results: </strong>A definitive screening design identified ultrasonic time, temperature, incubation duration, and anaerobic-aerobic treatment as the key factors influencing GABA accumulation. Central composite design optimization determined the optimal conditions as: ultrasonic frequency 20/35 kHz, ultrasonic time 7.3 min, temperature 42 °C, pH 7.2, power density 12 W L^-1, and three cycles of 2 h anaerobic followed by 1 h aerobic treatment. Under these conditions, GABA content in treated mulberry leaves (UAML) increased 7.2-fold compared to the untreated control (CML). UAML also exhibited significant enhancements in total phenolic content (81.87%), 2,2'-azidinebis-3-ethylbenzothiazolin-6-sulfonic acid radical scavenging capacity (60.45%), 2,2-diphenyl-1-picrylhydrazyl scavenging capacity (154.09%), and angiotensin-converting enzyme inhibitory activity (71.76%). High-performance liquid chromatography (HPLC) analysis demonstrated significantly higher levels of 3-O-caffeoylquinic acid and rutin (P < 0.05) levels in UAML. Electrical conductivity of UAML was 8.22% higher than that of CML. Scanning electron microscopy visualized the increased wrinkles and open stomata on the surface of UAML, while fluorescent microscopy revealed elevated intracellular calcium ion (Ca²⁺) concentration in UAML.</p><p><strong>Conclusion: </strong>The combination of ultrasound and alternating anaerobic-aerobic treatment effectively enhanced GABA accumulation in mulberry leaves, providing a practical approach to producing value-added mulberry leaf products. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptides of corn oligopeptides improve Aβ_1-42-injured SHSY5Y cells.","authors":"Hanshuo Wu, Rui Liu, Xinxue Zhang, Hualei Wang, Ganlu Meng, Jie Ren, Wenying Liu, Shuguo Li","doi":"10.1002/jsfa.14307","DOIUrl":"https://doi.org/10.1002/jsfa.14307","url":null,"abstract":"<p><strong>Background: </strong>There are more and more Alzheimer's patients. The formation of plaques of Aβ_1-42 in the brain is one of the main causes of Alzheimer's disease. Corn oligopeptides have natural antioxidant effects. It is aimed to develop functional corn oligopeptides to prevent Alzheimer's disease through antioxidation.</p><p><strong>Methods: </strong>According to previous laboratory studies, peptides of corn oligopeptides were screened by biological activity score and ADMET prediction, and molecular docking technology was used to screen the peptides that had high binding energy with Aβ_1-42. The protective effects of the selected peptides were evaluated against oxidative stress in Aβ_1-42-injured SHSY5Y cells, and the mechanism of the effects in the protein kinase A (PKA)/cAMP-response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF)-mediated signaling pathway was investigated, which is closely related to neurodegenerative and neurological diseases.</p><p><strong>Results: </strong>The results showed that three peptides (GL, FA and FQ) significantly increased the cell viability of Aβ_1-42-induced cells and mitochondrial intensity and decreased extracellular lactate dehydrogenase content. They also improved intracellular oxidative stress caused by Aβ_1-42, including reducing the overproduction of intracellular reactive oxygen species, and increasing the content of lipid oxidation, superoxide dismutase and glutathione peroxidase. In addition, western blot showed that treatment with GL, FA and FQ significantly increased the expression of PKA, CREB and BDNF, whereas cells injured with Aβ_1-42 decreased the expression of these signaling proteins.</p><p><strong>Conclusion: </strong>These results suggest that peptides of corn oligopeptides can effectively improve Aβ_1-42-induced Alzheimer's disease, and may improve oxidative stress response to protective nerve cells by up-regulating the protein expression of the PKA/CREB/BDNF signaling pathway. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial transformations of bioactives in fermented foods","authors":"Apramita Devi,&nbsp;H.P. Vasantha Rupasinghe,&nbsp;Amit Kumar Rai","doi":"10.1002/jsfa.14284","DOIUrl":"https://doi.org/10.1002/jsfa.14284","url":null,"abstract":"","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":"105 8","pages":"4057"},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to 'Organic zinc and manganese enhance eggshell ultrastructure by influencing its calcium deposition to improve eggshell quality in laying hens'.","authors":"","doi":"10.1002/jsfa.14350","DOIUrl":"https://doi.org/10.1002/jsfa.14350","url":null,"abstract":"","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential of spore surface-displayed keratinase for feather waste degradation using high-throughput screening.","authors":"Mingchen Yan, Zhen Wang, Huimin Zhou, Yanzhen Chen, Muhammad Saeed, Yudan Xu, Ying Chen, Zhong Ni, Zhen Fang, Huayou Chen","doi":"10.1002/jsfa.14361","DOIUrl":"https://doi.org/10.1002/jsfa.14361","url":null,"abstract":"<p><strong>Background: </strong>With the development of the global poultry industry, millions of tons of feathers are produced each year. A significant proportion is improperly discarded, leading to a substantial loss of keratin resources from feathers. Utilizing feathers in the feed industry could reduce costs substantially. However, the new fermented feed industry lacks acid-resistant keratinase, making it unable to convert feather keratin effectively in a lactic acid bacteria fermentation environment.</p><p><strong>Results: </strong>This study established a high-throughput screening method using spore surface-display to identify acid-resistant mutants generated by error-prone PCR. A mutant H4 (N43K/N143I) was obtained with a significant increase in activity under acidic conditions, and it was 1.34-fold more active than the wild type in a pH 5 environment. Bioinformatic analysis showed that the increase in stress resistance and activity of H4 was due to an increase in certain weak interaction forces and alterations in energy levels. The addition of H4 spore suspension as an enzyme preparation to feather meal fermentation feed with the participation of lactic acid bacteria improved the content of small peptides and free amino acids significantly. After being subjected to short-term high-temperature treatments, the H4 spore preparation was still able to degrade chicken feathers.</p><p><strong>Conclusion: </strong>These results suggest that the spore surface-displayed keratinase mutant H4 has potential for improved effectiveness in feather degradation within the fermented feed industry. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143971444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid, simple and accessible phytonutritional assessment methods in agricultural research.","authors":"Zipora Tietel, Izabella Galilov, Sarit Melamed","doi":"10.1002/jsfa.14363","DOIUrl":"https://doi.org/10.1002/jsfa.14363","url":null,"abstract":"<p><strong>Background: </strong>Agricultural research with respect to studying the effects of genetic background and environmental conditions on plant and commercial parameters is ample. Nevertheless, data on the effects on nutritional value and phytonutritional composition are scarce, despite its significance to the consumer, as well as its importance in providing nutritional security. The present study aimed to simplify, unify and make accessible a set of spectrophotometric nutritional assessment assays, comprising a valuable tool in agricultural research. Efforts were made to provide simple, rapid, small-scale and cost-effective protocols, aiming to minimize resources and time, learning-curve and erring.</p><p><strong>Results: </strong>We included assays alongside literature-compliant values for antioxidant capacity (ABTS, DPPH, FRAP), polyphenols and flavonoids (free, conjugated, bound, total), lignans, anthocyanins, tocopherols, phytosterols, glucosinolates, carotenoids, betalains, saponins, phlorotannin, phycobilin, vitamin C, total oil and soluble protein content, in addition to antinutrients- oxalic acid and phytic acid. Calibration curves of recommended standards are included and workflows are demonstrated. Guidelines are also provided for protocol adjustments.</p><p><strong>Conclusion: </strong>This work thus compiles a broad arsenal of rigorous methods, rendering a good spectrum of nutritional assessment protocols, including nutriphytochemical composition alongside a basic bioactivity assessment (antioxidant measurements). These methods are recommended in evaluating the effects of agricultural management practices, such as water and mineral nutrition availability or postharvest storage, or as part of breeding programs, such as in research or commercial R&D laboratories that do not have expertise in nutritional analysis, and require an accessible high-throughput nutritional assessment tool. © 2025 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144008869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the genetic diversity, seed oil fatty acid constituents and antioxidant activity of different okra (Abelmoschus esculentus (L.) Moench) populations.","authors":"Seyed Mehdi Talebi, Asra Salman, Masoumeh Najibzadeh, Mansour Ghorbanpour","doi":"10.1002/jsfa.14334","DOIUrl":"https://doi.org/10.1002/jsfa.14334","url":null,"abstract":"<p><strong>Background: </strong>Abelmoschus esculentus (L.) Moench is cultivated globally for use as a salad or culinary herb. It has a narrow genetic diversity and is sensitive to heat and drought stress. Therefore, it is very important to detect different chemotypes and genotypes to be applied in its breeding programs.</p><p><strong>Results: </strong>The genetic diversity is equally distributed within and among the populations. Principal coordinates analysis, genetic diversity and polymorphism parameters revealed a high level of within-population diversity for some populations. PhiPT values, genetic differentiation index and total heterozygosity amount explored a high level of genetic differentiation between some populations, with a low average rate of gene flow. Nei's genetic distance and STRUCTURE analysis assigned the populations to two distinct and some intermediate genotype groups. Seed oils were composed of the same unsaturated and saturated fatty acids in a ratio of nearly 2:1. Linoleic and oleic fatty acids were the first and second main unsaturated fatty acids, followed by palmitic acid as a major saturated fatty acid in all populations. Clustering analyses explored the populations classified into two chemotype groups. However, it was not similar to those obtained by the molecular genetic data. 2,2-Diphenyl-1-picrylhydrazyl (DPPH; IC₅₀ μg mL^-1) analysis revealed a moderate to weak antioxidant activity for a few populations, which significantly correlated with palmitic acid amount.</p><p><strong>Conclusion: </strong>Environmental factors have a stronger effect on seed oil fatty acids profiles than the genetic structure. The detected genotypes and chemotypes could be used for the development of genetic and chemical diversity, respectively, in this crop. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the antimicrobial activity of fermented and non-fermented cocoa bean shell extracts through metabolomics analysis and synergistic studies.","authors":"Yie Kie Chong, Wei Khang Gan, Joash Ban Lee Tan, Ahmad Kamil Hj Mohd Jaaffar, Zainal Baharum, Keng Yoon Yeong","doi":"10.1002/jsfa.14366","DOIUrl":"https://doi.org/10.1002/jsfa.14366","url":null,"abstract":"<p><strong>Background: </strong>Cocoa bean shell (CBS) extracts have emerged as a promising source of antimicrobial compounds. However, the bioactive compounds responsible for their antimicrobial activity have not been studied sufficiently. This study analyzed the antimicrobial properties of 12 extracts from fermented and non-fermented CBS, employing solvent and steam distillation extraction techniques to maximize bioactive diversity. The extracts were assessed against selected bacteria and fungi, including the 'ESKAPE' pathogens.</p><p><strong>Results: </strong>The CBS solvent and steam distillation extracts (except for ethyl acetate-fermented CBS) were found to exhibit antimicrobial activity against Streptococcus mutans with minimum inhibitory concentrations (MICs) as low as 0.0625 mg mL^-1, whereas both fermented and non-fermented steam distillation CBS extracts were found to be active against Candida albicans with MICs at 1 mg mL^-1. Overall, steam distillation extracts possessed enhanced antimicrobial activity in comparison with solvent extracts of CBS. Fermented CBS extracts were found to possess better antimicrobial activity than non-fermented CBS extracts. Metabolomics analysis identified theobromine (TB) and tetramethylpyrazine (TMP) as molecules that contributed to antimicrobial activity against S. mutans. Results showed that caffeine (CAF), TB and TMP were active against S. mutans and Acinetobacter baumannii, whereas CAF and TB were active against C. albicans. Significant synergistic effects of CAF, TB, and TMP with ciprofloxacin (CIP) were observed against Klebsiella aerogenes.</p><p><strong>Conclusion: </strong>These findings highlight the significant potential of bioactive compounds present in CBS for use in the development of sustainable antimicrobial agents. These naturally occurring compounds, including CAF, TB, and TMP, exhibited notable antimicrobial properties, which could also be harnessed to enhance the activity of commonly used antibiotics such as ciprofloxacin. Future studies could focus on determining the mode of action of these bioactive molecules. © 2025 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yeast culture promotes albumen quality by improving magnum protein secretion and intestinal microbiota in aged laying hens.","authors":"Li-Bing Gao, Dong Dai, Peng Chen, Hai-Jun Zhang, Shu-Geng Wu, Guang-Hai Qi, Jing Wang","doi":"10.1002/jsfa.14364","DOIUrl":"https://doi.org/10.1002/jsfa.14364","url":null,"abstract":"<p><strong>Background: </strong>The supplementation of yeast culture (YC) has the potential to enhance egg quality in laying hens. However, most studies focus on eggshell quality. This study aimed to investigate the effects of dietary YC supplementation on the production performance, albumen quality, protein synthesis or secretion of the magnum and the cecal microbiota content of laying hens.</p><p><strong>Results: </strong>The results showed that dietary YC supplementation increased albumen height and Haugh unit (P ≤ 0.05). Besides, dietary 100 g kg^-1 YC addition increased significantly the ridge width of the magnum and the relative expression of SEC23A in the magnum and decreased significantly the relative expression of OVOB in the magnum (P ≤ 0.05). Furthermore, the abundances of Butyricicoccus, Alistipes and Flavonifractor were increased significantly by 100 g kg^-1 YC supplementation (P ≤ 0.05). The diet supplemented with 100 g kg^-1 YC significantly increased the butyric acid and isobutyric acid of the cecum.</p><p><strong>Conclusion: </strong>Dietary supplementation with YC improved protein secretion in the magnum and enhanced the beneficial cecal microbiota, thus improving the albumen quality of laying hens. © 2025 Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon footprint for farms in the Czech Republic: a benchmark-based assessment.","authors":"Jan Kovanda, Svatava Janoušková, Tomáš Hák, Viktor Třebický, Petr Koňata","doi":"10.1002/jsfa.14368","DOIUrl":"https://doi.org/10.1002/jsfa.14368","url":null,"abstract":"<p><strong>Background: </strong>Climate change is a pressing environmental and social challenge that demands effective monitoring of greenhouse gas (GHG) emissions. One widely adopted approach for this is quantifying the carbon footprint (CF). Given that agriculture is a major contributor to GHG emissions, we have developed a comprehensive framework for CF accounting at the farm level. This framework has been tested on 12 farms in the Czech Republic to assess both data availability and calculation accuracy.</p><p><strong>Results: </strong>Our study examines how various farm characteristics, such as turnover, land area and number of employees, influence the overall CF and enable meaningful comparisons between farms. We found that absolute farm CFs are significantly influenced by the size effect, making them unsuitable for benchmarking purposes. By contrast, relative farm CFs (per turnover, per area and per employee) are not affected by the size effect, but can be affected by a scale effect. Additionally, we investigated whether a focus on animal husbandry leads to higher relative CFs. By calculating the share of animal husbandry (SoAH) in farm operations, we discovered a significant correlation between SoAH and relative CFs, with the strongest correlation observed for CF per turnover (0.87).</p><p><strong>Conclusion: </strong>We argue that farms with high shares of SoAH are unlikely to reduce their relative CFs to the levels of farms with zero or low SoAH. We therefore propose applying benchmarking to farms with similar SoAH. We also propose that further research should focus on defining and validating relevant reference values, comprising a benchmark set that reflects different farm types. © 2025 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</p>","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}