石榴皮提取物作为多酚氧化酶的生物活性抑制剂：延长新鲜农产品保存的绿色替代品。

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-09-30 DOI:10.1002/jsfa.70211

Hatice Paluzar, Sebnem Mutlu, Aysen Suekinci Yilmaz, Sebnem Selen Isbilir

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引用次数: 0

摘要

背景：天然植物提取物，特别是那些富含黄烷醇的植物提取物，是防止新鲜水果和蔬菜酶促褐变的有希望的替代品。石榴皮是一种丰富的农业工业副产品，具有抗氧化和抑制酶活性的生物活性化合物的潜在来源。研究了Hicaz石榴果皮酚类提取物对多酚氧化酶（PPO）的抑制作用及其对鲜切品品质特性的影响。结果：超声辅助提取提取液，得率为33.55%，采用液相色谱-串联质谱法对提取液进行分析，发现7种主要酚类化合物。分子对接研究证实了与PPOs的强结合相互作用。酶抑制试验表明，马铃薯，蘑菇和苹果PPOs的IC₅0值分别为0.86,0.95和0.73 mg mL-1，表明混合型抑制机制。用2mg mL-1提取物处理的新鲜切片样品在4°C下保存，马铃薯和苹果的保存时间可达7天，蘑菇的保存时间可达3天，保持品质属性并减少褐变。结论：研究结果表明，Hicaz石榴皮提取物是一种有效的天然PPO抑制剂，能够延长鲜切农产品的保质期。其在食品保鲜方面的潜在应用突出了其作为废物来源资源的经济价值及其对可持续食品加工的贡献。©2025化学工业协会。

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Pomegranate peel extract as a bioactive inhibitor of polyphenol oxidase: a green alternative for extending fresh-cut produce preservation.

Background: Natural plant extracts, particularly those rich in flavanols, are promising alternatives to prevent enzymatic browning in fresh-cut fruits and vegetables. Pomegranate peel, an abundant agro-industrial byproduct, has been recognized as a potential source of bioactive compounds with antioxidant and enzyme inhibitory activities. This study investigates the inhibitory effects of a phenolic extract from the peel of Hicaz variety pomegranate on polyphenol oxidase (PPO) and its impact on the quality attributes of fresh-cut produce.

Results: The extract, obtained by ultrasound-assisted extraction with a yield of 33.55%, was analyzed using liquid chromatography-tandem mass spectrometry, revealing seven major phenolic compounds. Molecular docking studies confirmed strong binding interactions with PPOs. Enzyme inhibition assays demonstrated IC₅₀ values of 0.86, 0.95 and 0.73 mg mL^-1 for potato, mushroom and apple PPOs, respectively, indicating a mixed-type inhibition mechanism. Fresh-cut samples treated with 2 mg mL^-1 extract and stored at 4 °C showed improved preservation, maintaining quality attributes and reducing browning for up to 7 days in potato and apple, and up to 3 days in mushroom.

Conclusion: The findings demonstrate that Hicaz pomegranate peel extract is an effective natural PPO inhibitor capable of extending the shelf life of fresh-cut produce. Its potential applications in food preservation highlight both its economic value as a waste-derived resource and its contribution to sustainable food processing. © 2025 Society of Chemical Industry.

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.