唾液酸和3′-唾液乳糖对ecc激菌IPEC-J2细胞粪便菌群发酵和益生元活性的影响

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-05-19 DOI:10.1002/jsfa.14372

Qiwen Zhou, Shuhuan Lu, Kejue Feng, Kai Na, Li Xiang, Min Xiao, Shuang Lu, Li Zhang, Xiangyu Li, Xiaohua Guo

{"title":"唾液酸和3′-唾液乳糖对ecc激菌IPEC-J2细胞粪便菌群发酵和益生元活性的影响","authors":"Qiwen Zhou, Shuhuan Lu, Kejue Feng, Kai Na, Li Xiang, Min Xiao, Shuang Lu, Li Zhang, Xiangyu Li, Xiaohua Guo","doi":"10.1002/jsfa.14372","DOIUrl":null,"url":null,"abstract":"Background: Sialic acid (SA) and 3'-sialyllactose (3'-SL) are potential food supplements, capable of promoting gut health and brain development. Structurally, 3'-SL can be formed by coupling a sialyl moiety to lactose via α-2,3 glycosidic linkages. However, there is currently no conclusive evidence regarding the role of the sialyl moiety in the prebiotic function of 3'-SL or its contribution to the biological activity of 3'-SL itself.Results: This study demonstrated that SA and 3'-SL differentially modulate the gut microbial community structure during in vitro fermentation, selectively promoting the growth of specific microbiota and altering short-chain fatty acid production profiles. Specifically, SA significantly increased acetate levels whereas 3'-SL mainly elevated the production of butyrate. At the cellular level, both compounds consistently strengthened intestinal barrier integrity in IPEC-J2 cells, reduced apoptosis, and alleviated oxidative stress induced by enterotoxigenic Escherichia coli.Conclusion: This study highlights the prebiotic potential of SA and 3'-SL in maintaining intestinal homeostasis, an effect likely mediated by their distinct structural properties. © 2025 Society of Chemical Industry.","PeriodicalId":17725,"journal":{"name":"Journal of the Science of Food and Agriculture","volume":" ","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparative effect of sialic acid and 3'-sialyllactose on fecal microbiota fermentation and prebiotic activity in ETEC-challenged IPEC-J2 cells.\",\"authors\":\"Qiwen Zhou, Shuhuan Lu, Kejue Feng, Kai Na, Li Xiang, Min Xiao, Shuang Lu, Li Zhang, Xiangyu Li, Xiaohua Guo\",\"doi\":\"10.1002/jsfa.14372\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Sialic acid (SA) and 3'-sialyllactose (3'-SL) are potential food supplements, capable of promoting gut health and brain development. Structurally, 3'-SL can be formed by coupling a sialyl moiety to lactose via α-2,3 glycosidic linkages. However, there is currently no conclusive evidence regarding the role of the sialyl moiety in the prebiotic function of 3'-SL or its contribution to the biological activity of 3'-SL itself.Results: This study demonstrated that SA and 3'-SL differentially modulate the gut microbial community structure during in vitro fermentation, selectively promoting the growth of specific microbiota and altering short-chain fatty acid production profiles. Specifically, SA significantly increased acetate levels whereas 3'-SL mainly elevated the production of butyrate. At the cellular level, both compounds consistently strengthened intestinal barrier integrity in IPEC-J2 cells, reduced apoptosis, and alleviated oxidative stress induced by enterotoxigenic Escherichia coli.Conclusion: This study highlights the prebiotic potential of SA and 3'-SL in maintaining intestinal homeostasis, an effect likely mediated by their distinct structural properties. © 2025 Society of Chemical Industry.\",\"PeriodicalId\":17725,\"journal\":{\"name\":\"Journal of the Science of Food and Agriculture\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2025-05-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of the Science of Food and Agriculture\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1002/jsfa.14372\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Science of Food and Agriculture","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1002/jsfa.14372","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

摘要

背景：唾液酸（SA）和3'-唾液基乳糖（3'-SL）是潜在的食物补充剂，能够促进肠道健康和大脑发育。结构上，3 '-SL可以通过α-2,3糖苷键与乳糖偶联而形成。然而，目前还没有确凿的证据表明唾液酸部分在3'-SL的益生元功能中所起的作用或其对3'-SL本身的生物活性的贡献。结果：本研究表明，SA和3′-SL在体外发酵过程中对肠道微生物群落结构进行了差异调节，选择性地促进了特定微生物群的生长，改变了短链脂肪酸的产生谱。其中，SA显著提高了乙酸水平，而3′-SL主要提高了丁酸的产量。在细胞水平上，这两种化合物均能增强IPEC-J2细胞的肠道屏障完整性，减少细胞凋亡，减轻肠道产毒素大肠杆菌诱导的氧化应激。结论：本研究强调了SA和3'-SL在维持肠道稳态方面的益生元潜力，这种作用可能是由它们独特的结构特性介导的。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Comparative effect of sialic acid and 3'-sialyllactose on fecal microbiota fermentation and prebiotic activity in ETEC-challenged IPEC-J2 cells.

Background: Sialic acid (SA) and 3'-sialyllactose (3'-SL) are potential food supplements, capable of promoting gut health and brain development. Structurally, 3'-SL can be formed by coupling a sialyl moiety to lactose via α-2,3 glycosidic linkages. However, there is currently no conclusive evidence regarding the role of the sialyl moiety in the prebiotic function of 3'-SL or its contribution to the biological activity of 3'-SL itself.

Results: This study demonstrated that SA and 3'-SL differentially modulate the gut microbial community structure during in vitro fermentation, selectively promoting the growth of specific microbiota and altering short-chain fatty acid production profiles. Specifically, SA significantly increased acetate levels whereas 3'-SL mainly elevated the production of butyrate. At the cellular level, both compounds consistently strengthened intestinal barrier integrity in IPEC-J2 cells, reduced apoptosis, and alleviated oxidative stress induced by enterotoxigenic Escherichia coli.

Conclusion: This study highlights the prebiotic potential of SA and 3'-SL in maintaining intestinal homeostasis, an effect likely mediated by their distinct structural properties. © 2025 Society of Chemical Industry.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.