Peptides of corn oligopeptides improve Aβ_1-42-injured SHSY5Y cells.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-05-15 DOI:10.1002/jsfa.14307

Hanshuo Wu, Rui Liu, Xinxue Zhang, Hualei Wang, Ganlu Meng, Jie Ren, Wenying Liu, Shuguo Li

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引用次数: 0

Abstract

Background: There are more and more Alzheimer's patients. The formation of plaques of Aβ_1-42 in the brain is one of the main causes of Alzheimer's disease. Corn oligopeptides have natural antioxidant effects. It is aimed to develop functional corn oligopeptides to prevent Alzheimer's disease through antioxidation.

Methods: According to previous laboratory studies, peptides of corn oligopeptides were screened by biological activity score and ADMET prediction, and molecular docking technology was used to screen the peptides that had high binding energy with Aβ_1-42. The protective effects of the selected peptides were evaluated against oxidative stress in Aβ_1-42-injured SHSY5Y cells, and the mechanism of the effects in the protein kinase A (PKA)/cAMP-response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF)-mediated signaling pathway was investigated, which is closely related to neurodegenerative and neurological diseases.

Results: The results showed that three peptides (GL, FA and FQ) significantly increased the cell viability of Aβ_1-42-induced cells and mitochondrial intensity and decreased extracellular lactate dehydrogenase content. They also improved intracellular oxidative stress caused by Aβ_1-42, including reducing the overproduction of intracellular reactive oxygen species, and increasing the content of lipid oxidation, superoxide dismutase and glutathione peroxidase. In addition, western blot showed that treatment with GL, FA and FQ significantly increased the expression of PKA, CREB and BDNF, whereas cells injured with Aβ_1-42 decreased the expression of these signaling proteins.

Conclusion: These results suggest that peptides of corn oligopeptides can effectively improve Aβ_1-42-induced Alzheimer's disease, and may improve oxidative stress response to protective nerve cells by up-regulating the protein expression of the PKA/CREB/BDNF signaling pathway. © 2025 Society of Chemical Industry.

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玉米寡肽肽可改善a β1-42损伤的SHSY5Y细胞。

背景：阿尔茨海默病患者越来越多。大脑中Aβ1-42斑块的形成是阿尔茨海默病的主要原因之一。玉米寡肽具有天然的抗氧化作用。目的是开发功能性玉米寡肽，通过抗氧化来预防阿尔茨海默病。方法：根据前期实验室研究，通过生物活性评分和ADMET预测筛选玉米寡肽肽段，并采用分子对接技术筛选与Aβ1-42结合能高的肽段。评价所选多肽对Aβ1-42损伤的SHSY5Y细胞氧化应激的保护作用，并探讨其在与神经退行性和神经系统疾病密切相关的蛋白激酶A (PKA)/ camp反应元件结合蛋白(CREB)/脑源性神经营养因子（BDNF）介导的信号通路中的作用机制。结果：三种多肽（GL、FA和FQ）显著提高了a β1-42诱导的细胞活力和线粒体强度，降低了细胞外乳酸脱氢酶含量。它们还能改善a - β1-42引起的细胞内氧化应激，包括减少细胞内活性氧的过量产生，增加脂质氧化、超氧化物歧化酶和谷胱甘肽过氧化物酶的含量。此外，western blot结果显示，GL、FA和FQ处理显著增加了PKA、CREB和BDNF的表达，而Aβ1-42损伤的细胞则降低了这些信号蛋白的表达。结论：玉米寡肽肽可有效改善a β1-42诱导的阿尔茨海默病，并可能通过上调PKA/CREB/BDNF信号通路蛋白表达来改善保护性神经细胞的氧化应激反应。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.