Journal of Veterinary Diagnostic Investigation最新文献_第8页

Aortic body tumor with intracardiac metastasis in a dog. 犬主动脉体肿瘤合并心内转移一例。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1177/10406387241304438

Matthew I Crawford-Jennings, Lezith D Chavez, Emma R Loessberg, Francisco R Carvallo-Chaigneau

引用次数: 0

Intoxication of sheep by Astragalus arequipensis in northwestern Argentina. 阿根廷西北部羊被黄芪中毒。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-27 DOI: 10.1177/10406387241311815

Raul E Marin, Dale Gardner, Daniel Cook, Anibal G Armien, Renée H Fortunato, Franklin Riet-Correa, Francisco A Uzal

{"title":"Intoxication of sheep by Astragalus arequipensis in northwestern Argentina.","authors":"Raul E Marin, Dale Gardner, Daniel Cook, Anibal G Armien, Renée H Fortunato, Franklin Riet-Correa, Francisco A Uzal","doi":"10.1177/10406387241311815","DOIUrl":"10.1177/10406387241311815","url":null,"abstract":"Spontaneous intoxication by Astragalus arequipensis was diagnosed in a flock of 300 sheep in Jujuy province, northwestern Argentina, that grazed an area heavily invaded by this plant. The main clinical signs were intention tremors, ataxia, and progressive loss of condition. Autopsy of 2 affected animals revealed loss of body condition. The main microscopic changes were fine cytoplasmic vacuolation of cells in the cerebrum, cerebellum, thyroid and adrenal glands, kidney, liver, pancreas, urinary bladder, and lymph nodes, and swollen axons in the cerebellum. Ultrastructurally, the cytoplasmic vacuoles consisted of dilated secondary lysosomes. Composite leaf and stem samples of A. arequipensis analyzed by high-performance liquid chromatography contained 0.05% swainsonine. The diagnosis of intoxication by A. arequipensis was made based on the clinical history and signs; gross, microscopic, and ultrastructural changes; and detection of swainsonine in the plant.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"375-379"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to: Oxytetracycline-resistant Paenibacillus larvae identified in commercial beekeeping operations in Saskatchewan using pooled honey sampling. 勘误表：在萨斯喀彻温省的商业养蜂业中使用混合蜂蜜取样鉴定出具有土霉素抗性的芽孢杆菌幼虫。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-10 DOI: 10.1177/10406387241308319

引用次数: 0

In vitro expression of genes encoding HIF1α, VEGFA, PGE2 synthases, and PGE2 receptors in feline oral squamous cell carcinoma. 猫口腔鳞状细胞癌中HIF1α、VEGFA、PGE2合成酶和PGE2受体基因的体外表达

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-02-10 DOI: 10.1177/10406387251315677

Walaa Hamed Shaker Nasry, Juan Carlos Rodriguez-Lecompte, Chelsea K Martin

{"title":"In vitro expression of genes encoding HIF1α, VEGFA, PGE2 synthases, and PGE2 receptors in feline oral squamous cell carcinoma.","authors":"Walaa Hamed Shaker Nasry, Juan Carlos Rodriguez-Lecompte, Chelsea K Martin","doi":"10.1177/10406387251315677","DOIUrl":"10.1177/10406387251315677","url":null,"abstract":"Feline oral squamous cell carcinoma (FOSCC) is an aggressive tumor with poor outcomes. Mechanisms of prostaglandin E2 (PGE2)-related inflammation and angiogenesis interact in human OSCC; however, this relationship has not been reported in FOSCC, to our knowledge. We aimed to characterize expression of genes encoding PGE2 synthases (PTGES1-3), PGE2 receptors (EP1-4), hypoxia inducible factor 1α (HIF1A), and vascular and endothelial growth factor A (VEGFA) in FOSCC cell lines (SCCF1-3) in vitro using reverse-transcription quantitative real-time PCR (RT-qPCR). Expression of PTGES1, PTGES3, EP4, and VEGFA were serum-inducible in SCCF2 cells; VEGFA was also inducible in SCCF1 cells (p ≤ 0.05). Compared to other serum-treated cells, SCCF3 cells had the lowest VEGFA expression despite the highest HIF1A (p ≤ 0.05) expression. PGE2 (5 µg/mL and 35 µg/mL) was added to SCCF2 cells for 4 different times (30, 60, 120, 240 min). Both doses of PGE2 stimulated expression of HIF1A and CD147 at 240 min (p ≤ 0.05). PGE2 treatment stimulated cyclooxygenase 2 (COX2) expression at 30 min, followed by suppression at 60 and 120 min and a sharp reduction in EP4 expression at 60 min (p ≤ 0.05). Treatment of SCCF2 with PGE2 and EP4 antagonist L-161,982 increased COX2 expression, and L-161,982 (alone and in combination with PGE2) stimulated EP4 expression (p ≤ 0.05). Genes for PGE2 synthase enzymes, PGE2 receptors, HIF1α and VEGFA were expressed in FOSCC cells in vitro. SCCF2 cells responded to exogenous PGE2 and EP4 antagonism, suggesting that EP4 activity in FOSCC deserves more study.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"223-233"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suspected fenugreek (Trigonella foenum-graecum L.) toxicosis in a herd of Saskatchewan beef cattle. 萨斯喀彻温省一群肉牛疑似葫芦巴（葫芦巴）中毒。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-09 DOI: 10.1177/10406387241307974

Vanessa E Cowan, Roman V Koziy, Laurie Zemlak, John R Campbell

{"title":"Suspected fenugreek (Trigonella foenum-graecum L.) toxicosis in a herd of Saskatchewan beef cattle.","authors":"Vanessa E Cowan, Roman V Koziy, Laurie Zemlak, John R Campbell","doi":"10.1177/10406387241307974","DOIUrl":"10.1177/10406387241307974","url":null,"abstract":"An apparent outbreak of fenugreek forage toxicosis occurred in a beef cattle herd near Moose Jaw, Saskatchewan in February-May 2022. The herd had consumed fenugreek hay from late fall to early winter. Clinical signs included various degrees of weakness, ataxia, knuckling, walking on hocks, and recumbency. All adult cattle in the herd eventually died or were euthanized. Feed analysis did not reveal nutritional deficiencies or mycotoxin contamination. Liver mineral and vitamin status of affected animals did not indicate any consistent abnormality. The last live cow in the herd was presented to a veterinary teaching hospital for evaluation and subsequent postmortem examination. Major postmortem findings included emaciation, and sciatic nerve and spinal cord axonal degeneration. Histologic examination of the sciatic nerve showed Wallerian-like axonal degeneration, increased Schwann cell nuclei, and endoneurial fibrosis. Histologic examination of the spinal cord showed infrequent myelin sheath dilation and digestion chambers within white matter. These results are consistent with other reports of natural and experimental outbreaks of fenugreek poisoning in livestock. To our knowledge, fenugreek toxicosis has not been reported previously in Canada. We conclude that caution should be taken when feeding fenugreek hay to cattle.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"329-333"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11719421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using HALO digital image analysis for automated detection of bovine viral diarrhea virus antigen in ear-notch specimens. 利用HALO数字图像分析技术自动检测牛病毒性腹泻病毒抗原耳缺口标本。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1177/10406387241307643

Susanne Je-Han Lin, Drew R Magstadt, Rachel J Derscheid, Eric R Burrough

{"title":"Using HALO digital image analysis for automated detection of bovine viral diarrhea virus antigen in ear-notch specimens.","authors":"Susanne Je-Han Lin, Drew R Magstadt, Rachel J Derscheid, Eric R Burrough","doi":"10.1177/10406387241307643","DOIUrl":"10.1177/10406387241307643","url":null,"abstract":"Detecting calves that are persistently infected with bovine viral diarrhea virus (BVDV) is essential to disease prevention. Immunohistochemistry (IHC) performed on formalin-fixed, paraffin-embedded ear-notch samples is commonly used for surveillance detection of BVDV antigens. However, due to the low percentage of positive samples in most submissions, the current workflow often entails considerable time reviewing negative results. Herein we aimed to utilize digital pathology and whole-slide imaging, coupled with advanced image analysis software, to enhance the efficiency of positive IHC detection in surveillance. Despite some challenges encountered during the implementation phase, the benefits of the reduced potential for human error and significant time savings for technicians and pathologists are evident. The screening of 518 slides, containing 2,884 ear notches, reached 97.4% sensitivity and 89.4% specificity compared to the gold standard of direct human assessment. The time taken for the personnel to operate the software and organize results was significantly shorter than the time needed for technicians and pathologists to manually examine the slides. Future refinements in software integration, staining protocols, and QC measures promise to further optimize this approach.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"354-357"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disseminated nontuberculous mycobacteriosis due to Mycobacterium avium subsp. hominissuis infection in a reindeer from a zoo. 由鸟分枝杆菌亚种引起的播散性非结核分枝杆菌病。动物园一头驯鹿感染人猿。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-28 DOI: 10.1177/10406387241307644

John M Winter, Jennifer N Langan, Jennifer A Landolfi, Tyler Thacker, Katie W Delk

{"title":"Disseminated nontuberculous mycobacteriosis due to Mycobacterium avium subsp. hominissuis infection in a reindeer from a zoo.","authors":"John M Winter, Jennifer N Langan, Jennifer A Landolfi, Tyler Thacker, Katie W Delk","doi":"10.1177/10406387241307644","DOIUrl":"10.1177/10406387241307644","url":null,"abstract":"Bacteria in the Mycobacterium tuberculosis complex and nontuberculous mycobacteria may affect a variety of animal species under human care and pose public health risks as zoonotic pathogens. A case of sudden onset of lethargy and increased respiratory effort in a 5-y-old, intact female reindeer (Rangifer tarandus) under managed care had progressed to severe dyspnea despite aggressive treatment. The animal was euthanized due to poor prognosis. Postmortem findings included: disseminated miliary nodules in the lungs, pleura, small intestine, liver, and spleen; enlarged mesenteric lymph nodes; dilated mesenteric and serosal lymphatic vessels; and renal infarcts. Histologically, granulomatous lymphadenitis and lymphangitis with intrahistiocytic acid-fast bacilli were observed. Mycobacterium sp. DNA was detected in lung via real-time PCR. Mycobacterial culture and sequencing identified Mycobacterium avium subsp. hominissuis (MAH) within pulmonary lesions. Infection with MAH has been reported in humans and many animal species; this nontuberculous mycobacterial infection may be an emerging concern in animals under managed care. To our knowledge, MAH infection has not been reported previously in reindeer.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"324-328"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11775928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute outbreak of polioencephalomalacia in adult beef cows on pasture associated with feeding broiler litter. 牧场上与饲养肉鸡窝粪有关的成年肉牛急性脑软化症暴发。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-02-02 DOI: 10.1177/10406387251315724

Savannah R Charnas, Timothy Walsh, Brandi Tharp, Scott A Fritz

{"title":"Acute outbreak of polioencephalomalacia in adult beef cows on pasture associated with feeding broiler litter.","authors":"Savannah R Charnas, Timothy Walsh, Brandi Tharp, Scott A Fritz","doi":"10.1177/10406387251315724","DOIUrl":"10.1177/10406387251315724","url":null,"abstract":"Polioencephalomalacia (PEM) is a neurologic disorder of ruminants that can affect cattle as a result of high levels of sulfur in the diet. Sulfur can be ingested by an animal through feed or water, and the mineral status of the animal can affect how much sulfur is ultimately absorbed. Broiler (poultry) litter is sometimes used in cattle diets as an economical protein source, although it runs the risk of supplying too much sulfur to the bovine diet. Here we report a case in which 15 cows had neurologic signs before death. Initial testing ruled out other causes of acute death, and histologic findings confirmed a diagnosis of PEM. The sulfur concentration was ~0.70% of the total mixed ration (TMR), which is above the maximum tolerable dietary concentration of sulfur. The broiler litter contributed nearly all of the sulfur to this ration and was the cause of the PEM experienced in this herd. When feeding by- and co-products as part of a TMR, it is important for producers to analyze the feed to mitigate risk and ensure good health.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"385-388"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11789040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptococcus hillyeri isolated from septic pleuritis in a horse. 从马脓毒性胸膜炎中分离出希利氏链球菌。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1177/10406387241306724

Ervin Albert, Imre Biksi, Levente Laczkó, László Miló, Karolina Cseri, Renáta Bőkényné Tóth, Dalma Papp, Dóra Halmay, Emese Bódai, Zoltán Bakos

{"title":"Streptococcus hillyeri isolated from septic pleuritis in a horse.","authors":"Ervin Albert, Imre Biksi, Levente Laczkó, László Miló, Karolina Cseri, Renáta Bőkényné Tóth, Dalma Papp, Dóra Halmay, Emese Bódai, Zoltán Bakos","doi":"10.1177/10406387241306724","DOIUrl":"10.1177/10406387241306724","url":null,"abstract":"Here we report the isolation of Streptococcus hillyeri from a thoracic sample from a horse. A 17-y-old Hungarian Sport Horse mare was referred to the equine clinic of the University of Veterinary Medicine Budapest, Hungary, with suspected pleuritis. Upon arrival, the horse was febrile and had tachycardia, severe inspiratory dyspnea, and tachypnea. Thoracic ultrasonography revealed severe bilateral pleural effusion, and a large area of lung consolidation. After sampling of both hemithoraces, 66 L of turbid exudate were drained. Based on these findings, a tentative diagnosis of septic pleuritis was made, and the horse was immediately started on a course of broad-spectrum antibiotics, a NSAID, an anticoagulant, and intravenous fluids. Despite intensive care, the clinical parameters deteriorated, and the horse was euthanized 6 d later. Cytology confirmed septic pleuritis, with short chains or groups of coccoid bacteria. Anaerobic culture yielded gram-positive cocci from both hemithoraces in almost pure culture, which we identified as S. hillyeri by 16S rDNA and whole-genome analysis. Additionally, we identified 4 previously unassigned Streptococcus sp. sequences as S. hillyeri. Of these, 3 were obtained from aborted equine fetuses and a fourth from a donkey mastitis case, supporting the pathogenic nature of S. hillyeri in these host species.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"393-397"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of a semiquantitative scoring system for mild-to-moderate gill lesions in Atlantic salmon reared in recirculating aquaculture systems in Norway. 挪威循循环水产养殖系统中大西洋鲑鱼轻度至中度鳃损伤的半定量评分系统评估。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-28 DOI: 10.1177/10406387241310900

Thomas Amlie, Alf Dalum, Marit Stormoen, Øystein Evensen

{"title":"Assessment of a semiquantitative scoring system for mild-to-moderate gill lesions in Atlantic salmon reared in recirculating aquaculture systems in Norway.","authors":"Thomas Amlie, Alf Dalum, Marit Stormoen, Øystein Evensen","doi":"10.1177/10406387241310900","DOIUrl":"10.1177/10406387241310900","url":null,"abstract":"Compromised gill health is a critical cause of forfeited welfare in Atlantic salmon farming. Detecting and quantifying the early onset of gill disease is important to reveal initial inflicting stimuli. We collected gill samples of 45 Atlantic salmon from 2 commercial recirculating aquaculture systems (RASs) spanning fry-to-market-size fish with no clinical signs of gill disease. Gill samples were assessed histologically by 3 independent raters with different levels of experience. Semiquantitative scoring for 7 types of gill changes was carried out for 10 filaments per gill (450 filaments total) over 3 rounds on anonymized samples. Scores were summarized for each type of gill change. The assumed clinical relevance for each change was transformed into a category score, followed by an assessment of agreement within (intra) and between (inter) raters. A generalized linear model estimated the difference in score levels between raters. For each rater, intra-rater agreement was high for 6 gill changes and moderate for 1 gill change. Inter-rater agreement was moderate to almost-perfect, except for 2 gill changes; generalized linear model regression revealed systematic differences in score usage between the raters. Our scoring protocol worked satisfactorily for mucous cell amount, lamellar clubbing, lamellar hypertrophy and/or hyperplasia, and aneurysms, despite different levels of expertise in histologic evaluation. Intra-rater agreement was consistent, but differences existed for interlamellar hypercellularity, lamellar inflammation, and degeneration. Scoring subclinical gill changes is a challenge, and our scoring system for mild-to-moderate lesions may enable early intervention to limit the detrimental effects of poor gill health in RAS farming.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"252-262"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11775946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0