Journal of Veterinary Diagnostic Investigation最新文献_第8页

Diagnosis of proliferative sparganosis caused by Spirometra decipiens complex 2 (Spirometra sp. 3) in a domestic cat in the United States. 美国一只家猫的增生性斯巴达病的诊断是由裂肺孢子虫复合物2（孢子虫sp. 3）引起的。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-03-21 DOI: 10.1177/10406387261432987

Amira Abdu, Emi Sasaki

{"title":"Diagnosis of proliferative sparganosis caused by Spirometra decipiens complex 2 (Spirometra sp. 3) in a domestic cat in the United States.","authors":"Amira Abdu, Emi Sasaki","doi":"10.1177/10406387261432987","DOIUrl":"10.1177/10406387261432987","url":null,"abstract":"Sparganosis, a zoonotic infection caused by the plerocercoid (sparganum) larval stage of Spirometra spp., is rarely reported in domestic cats. Sparganosis is typically seen as subcutaneous or visceral granulomatous lesions and has been associated with Spirometra mansonoides in North America. A proliferative form of sparganosis, with tissue invasion and widespread dissemination, has always been associated with Sparganum proliferum. However, emerging molecular evidence challenges this distinction. Here, we report a confirmed case of proliferative sparganosis in a domestic cat caused by Spirometra decipiens complex 2 (also referred to as Spirometra sp. 3). The cat had widespread lesions in multiple tissues, with gross and histologic lesions resembling those attributed to S. proliferum. Molecular identification of larval cestode DNA demonstrated a 99% match to S. decipiens, confirming its role in severe disseminated disease. Our case broadens the understanding of the pathogenic capacity of S. decipiens (Spirometra sp. 3) in felids and emphasizes the critical role of molecular detection for accurate species identification. To our knowledge, proliferative sparganosis attributable to Spirometra sp. 3 has not been reported previously in domestic cats. Given the zoonotic potential of sparganosis, our findings have important implications for both veterinary care and public health surveillance.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1055-1060"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13005755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147491322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of PCR and culture for detection of Campylobacter jejuni in canine feces. PCR与培养检测犬粪便空肠弯曲杆菌的比较。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-04-15 DOI: 10.1177/10406387261434179

Sara D Lawhon, Kevin J Cummings, Jing Wu, Ching Yuan Yang, Nicholas Whipple, Melanie Prarat Koscielny, Samantha Lindemann, Shannon Kiener, Matthew Kmet, Ravinder Reddy, Laura B Goodman, Rebecca J Franklin-Guild, Kelly Sams, Brittany Cronk, Jing Cui, Kerri Lawrence, Qirui Zhang, Yan Zhang, Steven Ensley, Karen Olsen, John Tomaszewski, Zhenyu Shen, Shuping Zhang, Sarmila Dasgupta, Shannon Mann, Amar Patil, Siddhartha Thakur, Shelley C Rankin, Laura Ruesch, Nuria Garcia-Fernandez, Jake Guag, Sarah M Nemser, Renate Reimschuessel

{"title":"Comparison of PCR and culture for detection of Campylobacter jejuni in canine feces.","authors":"Sara D Lawhon, Kevin J Cummings, Jing Wu, Ching Yuan Yang, Nicholas Whipple, Melanie Prarat Koscielny, Samantha Lindemann, Shannon Kiener, Matthew Kmet, Ravinder Reddy, Laura B Goodman, Rebecca J Franklin-Guild, Kelly Sams, Brittany Cronk, Jing Cui, Kerri Lawrence, Qirui Zhang, Yan Zhang, Steven Ensley, Karen Olsen, John Tomaszewski, Zhenyu Shen, Shuping Zhang, Sarmila Dasgupta, Shannon Mann, Amar Patil, Siddhartha Thakur, Shelley C Rankin, Laura Ruesch, Nuria Garcia-Fernandez, Jake Guag, Sarah M Nemser, Renate Reimschuessel","doi":"10.1177/10406387261434179","DOIUrl":"10.1177/10406387261434179","url":null,"abstract":"Campylobacter jejuni is a leading cause of human foodborne illness, and pet dogs can be a source of infection. Despite recognition of this zoonotic potential, tests for detection of C. jejuni are not performed routinely in dogs. We compared bacterial culture and molecular methods for the detection of C. jejuni directly from canine feces and from Bolton enrichment broth inoculated with feces in a multi-laboratory randomized-method test. Although culture offers the advantage of collection of isolates for further epidemiologic study, real-time PCR (rtPCR) to detect cpn60 from DNA isolated directly from canine feces was more sensitive than culture and more accurate than rtPCR using DNA isolated from enrichment cultures. The limit of detection for our cpn60 rtPCR was 320 cfu/g.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"891-899"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13083281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of antimicrobial susceptibility testing of Clostridium perfringens by agar dilution and broth microdilution methods. 琼脂稀释法与微量肉汤稀释法产气荚膜梭菌药敏试验比较。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-03-17 DOI: 10.1177/10406387261429091

Audrey Charlebois, Lila Maduro, Martine Boulianne

{"title":"Comparison of antimicrobial susceptibility testing of Clostridium perfringens by agar dilution and broth microdilution methods.","authors":"Audrey Charlebois, Lila Maduro, Martine Boulianne","doi":"10.1177/10406387261429091","DOIUrl":"10.1177/10406387261429091","url":null,"abstract":"In the poultry industry, antimicrobials are commonly used to prevent and treat bacterial infections. However, antimicrobial use in food-animal production has been implicated in the emergence of drug resistance in both pathogenic and non-pathogenic microorganisms. The Clinical and Laboratory Standards Institute (CLSI) suggests using the agar dilution method when testing for the antimicrobial susceptibility (AMS) of Clostridium perfringens, the causative agent of necrotic enteritis. Yet multiple studies on C. perfringens use the microdilution method, which is faster. Using the standard agar dilution and the microdilution methods, we obtained and compared the minimal inhibitory concentrations values of 64 field isolates of C. perfringens for 11 antimicrobials (amoxicillin, bacitracin, ceftiofur, erythromycin, monensin, narasin, penicillin, salinomycin, tetracycline, tylosin, and virginiamycin). Given the high variability in the values obtained for most of the antimicrobials by the microdilution method, we could not calculate the correlation coefficient between the 2 antimicrobial susceptibility techniques. Our study highlights the importance of following CLSI protocols when testing the AMS of C. perfringens.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1005-1008"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12999528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147474113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and preliminary application of a multiplex PCR method for the detection of goose parvovirus and goose astrovirus 1 and 2. 鹅细小病毒和鹅星形病毒1型和2型多重PCR检测方法的建立及初步应用。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-04-06 DOI: 10.1177/10406387261436326

Xinghui Song, Pengkun Yang, Xinyue Zhang, Jing Cao, Wenzhuo Huang, Zhifeng Peng, Li Zhao, Tingting Lu, Yanling Li, Xiaozhan Zhang

{"title":"Development and preliminary application of a multiplex PCR method for the detection of goose parvovirus and goose astrovirus 1 and 2.","authors":"Xinghui Song, Pengkun Yang, Xinyue Zhang, Jing Cao, Wenzhuo Huang, Zhifeng Peng, Li Zhao, Tingting Lu, Yanling Li, Xiaozhan Zhang","doi":"10.1177/10406387261436326","DOIUrl":"10.1177/10406387261436326","url":null,"abstract":"Goose astrovirus 1 (GAstV1), goose astrovirus 2 (GAstV2), and goose parvovirus (GPV) infections have significant negative effects on the goose farming industry. A rapid and accurate multiplex PCR method for simultaneous detection of these 3 goose viruses is needed. We designed and synthesized specific primers targeting the conserved regions of GAstV1, GAstV2, and GPV. and optimized reaction conditions to assess specificity and sensitivity. The optimal amplification condition was an annealing temperature of 54.5°C for 35 cycles. Specific bands were detected at 315 bp (GAstV1), 609 bp (GAstV2), and 1,405 bp (GPV). No cross-amplification occurred with other common goose pathogens, including avian influenza A virus H9, Newcastle disease virus, duck Tembusu virus, fowl adenovirus 4, goose reovirus, and goose circovirus. The limit of detection (LOD) for GAstV1, GAstV2, and GPV was 1 × 103 copies/μL, and the LOD for samples containing all 3 agents was 1 × 104 copies/μL. Our results from testing 90 clinical samples were in 100% concordance with single PCR assays, suggesting that our multiplex PCR assay offers a reliable and efficient approach for the clinical detection of GAstV1, GAstV2, and GPV.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"956-962"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13053386/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147623250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of DNA extraction methods to detect Pseudogymnoascus destructans in bat skin swabs, guano, and environmental swabs. 蝙蝠皮肤拭子、鸟粪拭子和环境拭子中破坏性假裸子菌DNA提取方法的比较。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-06-01 DOI: 10.1177/10406387261441588

Kyle G George, Jeffrey M Lorch, Anne E Ballmann

{"title":"Comparison of DNA extraction methods to detect Pseudogymnoascus destructans in bat skin swabs, guano, and environmental swabs.","authors":"Kyle G George, Jeffrey M Lorch, Anne E Ballmann","doi":"10.1177/10406387261441588","DOIUrl":"10.1177/10406387261441588","url":null,"abstract":"National surveillance for Pseudogymnoascus destructans-the fungus that causes white-nose syndrome in bats-relies on methods optimized for highly sensitive detection of the pathogen. However, comparisons of methods that inform assay functionality are sparse. We used a combination of conidia-spiking experiments and analysis of field-collected samples to compare methods for DNA extraction from bat guano, skin swabs, and environmental swabs for subsequent detection of P. destructans DNA via quantitative real-time PCR (qPCR). We evaluated 2 methods for all sample types and a third method for guano samples. We created conidia-inoculated guano samples from 3 guano sources, as well as conidia-inoculated environmental swabs using sediment from 7 bat hibernacula. Detection of P. destructans from environmental swabs was 2.94 times more likely from a purification-free method of DNA extraction, with up to 9.75 times more target DNA detected on average than a magnetic bead purification method, as evidenced by both laboratory-spiked and field-collected samples. We observed a similar result for skin swabs collected from P. destructans-infected bat carcasses, with a 31.7-fold increase in the amount of target DNA detected on average. Between the same 2 extraction methods, a 3.3-fold difference was found in the odds of detecting P. destructans from guano, but a third method designed for DNA extraction from soil had 12.6-41.8 times higher odds of detection. Our study further optimizes DNA extraction methods for the detection of P. destructans from environmental swabs and offers a detailed comparison of extraction methods used for detection from bat skin swabs and guano.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"908-917"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13226298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148138335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An illustrated guide to dissection and abdominal anatomy of the yellow mealworm beetle. 黄粉虫甲虫解剖和腹部解剖的图解指南。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-04-15 DOI: 10.1177/10406387261427763

Chloé Rosa-Teijeiro, Fanny Renois, Marie-Odile Benoit-Biancamano

{"title":"An illustrated guide to dissection and abdominal anatomy of the yellow mealworm beetle.","authors":"Chloé Rosa-Teijeiro, Fanny Renois, Marie-Odile Benoit-Biancamano","doi":"10.1177/10406387261427763","DOIUrl":"10.1177/10406387261427763","url":null,"abstract":"An unprecedented increase in the number and the size of yellow mealworm beetle (Tenebrio molitor) farms in North America and Europe has been driven, among other factors, by the demand for sustainable proteins. Insect farming offers ecologic advantages, including lower land and water use and reduced greenhouse gas emissions. As in conventional livestock farming, veterinarians are essential to maintain insect health because disease outbreaks can reduce productivity, compromise animal welfare, and cause monetary loss. Yet, the role of veterinary diagnosticians remains limited in this emerging sector because of the lack of standardized diagnostic tools and anatomic references. The autopsy of an invertebrate is a valuable tool that can, just as in any other species, serve to determine the cause of death, identify underlying disease processes, and guide herd health management. However, performing beetle autopsies is technically challenging given their small size, rigid exoskeleton, and fragile internal organs. Here, we offer an illustrated, step-by-step dissection protocol for adult T. molitor using standard laboratory equipment. Our method emphasizes an aqueous immersion approach that preserves tissue integrity, enhances visibility, and yields consistent results. We describe the abdominal anatomy of both sexes, including the digestive, reproductive, and nervous systems. Structures include 6 testicular follicles, bean-shaped accessory glands, and a spermathecal gland in females that exceeds the spermatheca in size. The abdominal nerve cord comprises 7 metameric ganglia, with caudal fusion.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"900-907"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13083270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suspected organophosphate delayed neurotoxicity in sheep associated with widespread lobed needlegrass (Nassella charruana)-induced skin perforations. 疑似有机磷延迟神经毒性与广泛的分叶针叶草（Nassella charruana）引起的皮肤穿孔有关。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-04-06 DOI: 10.1177/10406387261438463

Ana Lívia V Sousa, Ignacio Dutra, Rafael Martínez, Cintia R R Queiroz-Machado, Sergio Fierro, Saulo Díaz, Martín Jaurena, Franklin Riet-Correa, Alejo Menchaca, Mizael Machado

{"title":"Suspected organophosphate delayed neurotoxicity in sheep associated with widespread lobed needlegrass (Nassella charruana)-induced skin perforations.","authors":"Ana Lívia V Sousa, Ignacio Dutra, Rafael Martínez, Cintia R R Queiroz-Machado, Sergio Fierro, Saulo Díaz, Martín Jaurena, Franklin Riet-Correa, Alejo Menchaca, Mizael Machado","doi":"10.1177/10406387261438463","DOIUrl":"10.1177/10406387261438463","url":null,"abstract":"Organophosphate-induced delayed neuropathy (OPIDN) is a rare, chronic manifestation of organophosphate (OP) poisoning that typically emerges days after exposure and affects humans and other animals. Here, we describe the epidemiologic, clinical, and pathologic features of 2 suspected outbreaks of OPIDN in Australian Merino sheep in Uruguay following cutaneous exposure to OP. The outbreaks occurred in yearling sheep at 21 and 44 d after a single exposure to pirimiphos-methyl and diazinon, respectively, involving several hundred animals in each case. Affected animals had massive skin perforations caused by Nassella charruana (i.e., flechilla or lobed needlegrass)-a native grass of the Pampa biome that is common in Uruguayan natural grasslands-which likely facilitated enhanced dermal absorption of OP. Histologic examination revealed axonal spheroids, vacuolation, and variable numbers of digestion chambers in the cervical, lumbar, and sacrococcygeal spinal cord segments, predominantly involving the ventral horn, propriospinal tract, and dorsal and lateral funiculi. We suggest that the cutaneous lesions were a critical predisposing factor for OP absorption and the subsequent development of OPIDN. Management of native Nassella spp. in grazing areas may serve as a prophylactic strategy to mitigate the risk of OP toxicosis on sheep farms where dermal routes are used for antiparasitic treatments.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1102-1106"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13053395/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147623203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reference intervals of urinary metanephrines: creatinine ratios in dogs: comparison of acidified and non-acidified samples collected by cystocentesis. 尿肾上腺素的参考区间：狗的肌酐比率：膀胱穿刺收集的酸化和非酸化样品的比较。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-03-31 DOI: 10.1177/10406387261433471

Solène Niederlender, Kristina Museux, Maureen Lazard, Suzy Y M Valentin

{"title":"Reference intervals of urinary metanephrines: creatinine ratios in dogs: comparison of acidified and non-acidified samples collected by cystocentesis.","authors":"Solène Niederlender, Kristina Museux, Maureen Lazard, Suzy Y M Valentin","doi":"10.1177/10406387261433471","DOIUrl":"10.1177/10406387261433471","url":null,"abstract":"Canine pheochromocytomas remain difficult to diagnose, and reports of RIs for urinary metanephrines (metanephrine and normetanephrine) are scarce. Prior urinary metanephrines:creatinine (uMNs:Cr) ratio RIs are reported with free metanephrines and apply only to voided urine samples. We recruited 40 subclinical client-owned and staff-owned dogs from a veterinary hospital. Among those, 38 dogs were considered healthy based on normal bloodwork and adrenal glands of normal size on ultrasonographic examination. uMNs:Cr ratios were measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS) on urine samples collected by ultrasound-guided cystocentesis. We found no statistical difference between acidified and non-acidified urine samples. The RIs were 17-117 for the total metanephrine:Cr ratio and 37-223 nmol/mmol for the normetanephrine:Cr ratio. We confirmed that acidification of urine is not necessary. uMNs:Cr ratios can be measured in urine collected by cystocentesis. Metanephrines measurements should be included when investigating a suspicion of pheochromocytoma in dogs and can be requested easily by veterinarians in general practice.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"972-979"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13038486/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Skull-base chordoma in a dog. 狗的颅底脊索瘤。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-04-09 DOI: 10.1177/10406387261423949

Kana Hamamoto, Ikki Mitsui, Toshiyasu Matsui

{"title":"Skull-base chordoma in a dog.","authors":"Kana Hamamoto, Ikki Mitsui, Toshiyasu Matsui","doi":"10.1177/10406387261423949","DOIUrl":"10.1177/10406387261423949","url":null,"abstract":"Autopsy of a 16-y-old, castrated male Pomeranian dog revealed an incidental, friable, tan mass attached to the base of the skull. The mass stretched from the sella turcica to the foramen magnum, anatomically corresponding to the clivus. The mass neither firmly adhered to the cranium nor the medulla oblongata. No associated neurologic signs had been observed. Histologically, the mass had nests of round cells and islands of immature chondroid matrix. At the periphery of the chondroid matrix, physaliphorous cells with hyperchromatic nuclei were identified. Histochemical (von Kossa, Watanabe silver, Verhoeff-Van Gieson, and Masson trichrome stains; periodic acid-Schiff reaction) and immunohistochemistry (IHC; MUM1, CD3, CD20, IBA1, Ki67, S100, cytokeratin AE1/AE3, E-cadherin, vimentin, brachyury) procedures were performed. Immunohistochemically, neoplastic cells were positive for cytokeratin AE1/AE3, E-cadherin, vimentin, S100, and brachyury, and negative for MUM1, CD3, CD20, IBA1, and Ki67, supporting a diagnosis of chordoma. Our case highlights the potential for diverse morphology in chordomas and emphasizes the utility of an IHC panel, including anti-brachyury antibody, to aid in diagnosis. Our case also demonstrates the importance of evaluating the clivus during postmortem CNS examination.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1024-1027"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13065635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147639061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nasal carcinoma with leptomeningeal carcinomatosis in a dog. 犬鼻癌合并轻脑膜癌。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-01 Epub Date: 2026-04-08 DOI: 10.1177/10406387251410488

Maxwell Beecroft, Gilad Fefer, Will Patterson, Brigid Troan, Raisa Velez-Contreras

{"title":"Nasal carcinoma with leptomeningeal carcinomatosis in a dog.","authors":"Maxwell Beecroft, Gilad Fefer, Will Patterson, Brigid Troan, Raisa Velez-Contreras","doi":"10.1177/10406387251410488","DOIUrl":"10.1177/10406387251410488","url":null,"abstract":"A 7-y-old, castrated male Standard Poodle was presented to the North Carolina State University Small Animal Neurology Service (Raleigh, NC, USA) with a 2-wk history of progressive neurologic signs, including inappropriate mentation, ataxia, seizures, hyporexia, and nasal discharge. Neurologic examination indicated a forebrain lesion, and MRI revealed a large, contrast-enhancing mass centered at the right cribriform plate, extending into the cranial vault and causing brain compression. CSF analysis showed neutrophilic pleocytosis with neoplastic epithelial cells. Because of further clinical deterioration, the patient was euthanized. Postmortem examination revealed a large nasal mass extending into the brain and right orbit, with infiltration into the right olfactory lobe. Histologically, the mass was identified as an undifferentiated carcinoma with metastases along the leptomeninges as far caudal as the L3-L4 segment of the spinal cord. Nasal origin was supported by strong pan-cytokeratin and E-cadherin positivity and negative staining to synaptophysin, neural-specific enolase, S100, and CD18; partial positivity to vimentin suggested epithelial-to-mesenchymal transition in portions of the neoplasm. These findings confirmed leptomeningeal carcinomatosis secondary to the primary nasal carcinoma.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1009-1013"},"PeriodicalIF":1.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13065626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147638998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0