High prevalence and broad geographic distribution of urine shedding of bovine polyomavirus 1 (Epsilonpolyomavirus bovis) by cattle in Uruguay.

IF 1.2 3区 农林科学 Q3 VETERINARY SCIENCES
Matías Castells, Leticia Zarantonelli, Caroline da Silva Silveira, Rodney Colina, Federico Giannitti
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引用次数: 0

Abstract

In 2021, bovine polyomavirus 1 (BoPyV1; Polyomaviridae, Epsilonpolyomavirus bovis) was associated with nephritis in an aborted bovine fetus in Uruguay, with renal lesions resembling those typical of polyomavirus-associated nephropathy of humans. Given that little is known about the epidemiology of BoPyV1 infection in cattle, we screened for BoPyV1 in urine samples collected in 2015-2017 from beef and dairy herds from 12 of the 19 departments in Uruguay. We tested for BoPyV1 by PCR in 156 urine pools and 249 individual urine samples from 42 herds. We detected BoPyV1 in 33 of 42 (79%) farms across 100% of the departments studied, in similar proportions in beef (22 of 30; 73%) and dairy (11 of 12; 92%) herds. At the animal level, BoPyV1 was detected in 80 of 249 (32%) animals; this frequency was significantly higher in dairy (51 of 119; 43%) than beef (29 of 130; 22%) cattle, and in cows (36 of 81; 44%) than heifers (32 of 121; 26%). BoPyV1 strains circulating in Uruguay have a high degree (98.7-100%) of sequence identity at the major capsid protein VP1, which is slightly lower (96.2-99.7%) than for strains from other countries. We conclude that shedding of BoPyV1 in the urine of dairy and beef cattle is prevalent and geographically widespread in Uruguay.

乌拉圭牛尿中牛多瘤病毒 1(Epsilonpolyomavirus bovis)的高流行率和广泛地理分布。
2021年,牛多瘤病毒1型(BoPyV1;乌拉圭一例流产牛胎儿肾炎与多瘤病毒科(牛Epsilonpolyomavirus bovis)有关,其肾脏病变类似于人类多瘤病毒相关肾病的典型病变。鉴于对牛中BoPyV1感染的流行病学知之甚少,我们在2015-2017年从乌拉圭19个省中的12个省收集的牛肉和奶牛的尿液样本中筛查了BoPyV1。我们对来自42个畜群的156个尿池和249个个体尿液样本进行了BoPyV1 PCR检测。我们在所有被研究的部门的42个农场中的33个(79%)检测到BoPyV1,在牛肉中也有类似的比例(30个中的22个;73%)和乳制品(12人中有11人;92%)牛群。在动物水平上,249只动物中有80只(32%)检测到BoPyV1;这一频率明显高于乳制品(119人中有51人;43%)比牛肉(130人中有29人;22%)牛,81头牛中有36头;44%)比小母牛(121头中的32头;26%)。在乌拉圭流行的BoPyV1菌株在主要衣壳蛋白VP1上的序列同源性很高(98.7-100%),略低于其他国家的菌株(96.2-99.7%)。我们得出结论,在乌拉圭,乳牛和肉牛尿液中BoPyV1的脱落是普遍存在的,并且在地理上很广泛。
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来源期刊
CiteScore
3.00
自引率
6.70%
发文量
127
审稿时长
6-16 weeks
期刊介绍: The Journal of Veterinary Diagnostic Investigation (J Vet Diagn Invest) is an international peer-reviewed journal published bimonthly in English by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). JVDI is devoted to all aspects of veterinary laboratory diagnostic science including the major disciplines of anatomic pathology, bacteriology/mycology, clinical pathology, epidemiology, immunology, laboratory information management, molecular biology, parasitology, public health, toxicology, and virology.
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