Journal of Veterinary Diagnostic Investigation最新文献

Dirofilaria immitis antigen detection in the urine of dogs with known and unknown infection status.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-04-01 DOI: 10.1177/10406387251327415

Andrew C Brown, Meriam N Saleh, J Mack Fudge, Mary B Nabity, Guilherme G Verocai

{"title":"Dirofilaria immitis antigen detection in the urine of dogs with known and unknown infection status.","authors":"Andrew C Brown, Meriam N Saleh, J Mack Fudge, Mary B Nabity, Guilherme G Verocai","doi":"10.1177/10406387251327415","DOIUrl":"https://doi.org/10.1177/10406387251327415","url":null,"abstract":"Canine heartworm, Dirofilaria immitis, causes a potentially fatal, multisystemic disease in dogs. Diagnosis of heartworm disease relies on serologic antigen detection and microfilariae identification. Immune-complex dissociation (ICD) of serum or plasma by heat treatment increases detection. We assessed urine as a sample for heartworm antigen detection in dogs with known and unknown infection status using a commercial ELISA. Twenty-nine matching serum or plasma and urine samples were collected from 20 experimentally infected dogs to assess antigen detection in urine. Matching serum and urine samples of 267 dogs admitted to a spay/neuter clinic with unknown infection status were also screened. In experimentally infected dogs, antigenemia was detected in 86% of samples pre-ICD and 91% post-ICD; antigenuria was detected in 76% pre-ICD and 72% post-ICD; differences were not statistically significant. In clinic samples, antigenemia was detected in 7.9% of dogs pre-ICD and 12.4% post-ICD; antigenuria was detected in 11.6% of dogs pre-ICD and 6.4% post-ICD. In experimental infection samples, sensitivity (Se), specificity (Sp), and positive predictive value of antigenuria were high (85%, 100%, 100%, respectively). In clinic samples, Se and negative predictive value were high (95% and 92%, respectively). Our data confirm that heartworm antigen can be detected in the urine of dogs with both experimental heartworm infections and with unknown infection status. However, antigenuria had high Sp and low Se compared to matching serum of clinic dog samples and urine cannot be recommended for routine screening.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251327415"},"PeriodicalIF":1.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutaneous and subcutaneous fibrolipomas in 236 dogs, 2017-2024: a retrospective case series and comparative review.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-04-01 DOI: 10.1177/10406387251328600

Ricardo E Mendes, Weerapong Laovechprasit, Sarah M Schneider, Daniel R Rissi

引用次数: 0

Characterization of amyloid deposits in canine mammary neoplasms.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-04-01 DOI: 10.1177/10406387251328323

Yuka Kato, Natsumi Kobayashi, Susumu Iwaide, Shumpei Hisamoto, Satoshi Koyama, Yoshiyuki Itoh, Miki Hisada, Noboru Machida, Kohji Nomura, Tomoaki Murakami

{"title":"Characterization of amyloid deposits in canine mammary neoplasms.","authors":"Yuka Kato, Natsumi Kobayashi, Susumu Iwaide, Shumpei Hisamoto, Satoshi Koyama, Yoshiyuki Itoh, Miki Hisada, Noboru Machida, Kohji Nomura, Tomoaki Murakami","doi":"10.1177/10406387251328323","DOIUrl":"https://doi.org/10.1177/10406387251328323","url":null,"abstract":"Mammary tumor-associated amyloidosis in dogs has been reported in several studies, but its incidence and predisposing conditions have not been investigated. We examined 252 mammary masses from 183 dogs to determine the prevalence of amyloid deposition and elucidate etiologic factors. Histologically, amyloid deposition was observed in 88 of 252 (35%) mammary masses. Amyloid deposition patterns were categorized into 3 types: stromal deposition (ST), deposition in corpora amylacea (CA), and precipitation within luminal secretions (PR). Of the 88 amyloid deposition cases, 49 were intra-tumoral, especially for ST-type amyloid deposits, which were all intra-tumoral. Immunohistochemistry and mass spectrometry revealed that ST-type amyloid deposits were positive for either serum amyloid A or α-S1-casein, depending on the sample; all CA- and PR-type amyloid deposits were positive for α-S1-casein. Statistically, intra-tumoral ST-type amyloid deposition was significantly more frequent in histologic grade II (p = 0.009) or III (p < 0.001) mammary tumors. In contrast, amyloid deposition in extra-tumoral tissues is significantly associated with aging (p = 0.002). Our findings suggest that the disruption of proteostasis in the canine mammary gland associated with tumorigenesis and aging can readily induce amyloid formation.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251328323"},"PeriodicalIF":1.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of bovine anaplasmosis (Anaplasma marginale) with emphasis on epidemiology and diagnostic testing.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-28 DOI: 10.1177/10406387251324180

Rosalie A Ierardi

{"title":"A review of bovine anaplasmosis (Anaplasma marginale) with emphasis on epidemiology and diagnostic testing.","authors":"Rosalie A Ierardi","doi":"10.1177/10406387251324180","DOIUrl":"10.1177/10406387251324180","url":null,"abstract":"Bovine anaplasmosis, caused by the rickettsia Anaplasma marginale, is an economically important tick-borne disease of cattle that is found worldwide. Its clinical effects of severe anemia, decreased growth, weight loss, and death negatively impact cattle welfare and create a significant economic burden for cattle producers. Despite availability of highly sensitive and specific assays for anti-A. marginale antibodies (competitive ELISA) and A. marginale genetic material (real-time PCR), the interpretation of test results for the diagnosis of clinical anaplasmosis in cattle remains challenging. Treatment and control usually consist of administration of oral and/or injectable tetracyclines; however, this approach is unlikely to be sustainable in the face of increasing scrutiny of antimicrobial usage in livestock. Statistically robust prospective studies are needed to characterize the prevalence, distribution, and transmission of bovine anaplasmosis under field conditions, as the epidemiology of this disease remains incompletely understood. Apart from minimizing exposure of naïve cattle to carriers (e.g., testing new introductions and interpreting in the context of herd-level seropositivity, changing needles between cattle), veterinarians and producers have few tools for prevention of bovine anaplasmosis based on data-driven risk assessment. A vaccine that is consistently safe and effective has proved elusive, but ongoing research into A. marginale vaccine candidates offers hope for a more effective means of protecting cattle from this costly disease.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251324180"},"PeriodicalIF":1.2,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spontaneous branchioblastoma in a koi carp.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-28 DOI: 10.1177/10406387251324885

Benjamin Balducchi, Eileen Henderson

引用次数: 0

Adenofibroma of the uterine tube in a pig.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-28 DOI: 10.1177/10406387251324670

Diana A Bochynska, Corina G Toma, Sara Salgado, Ann P Donachey, Dana M Sheets-Nichols, Robert A Foster

引用次数: 0

Reference intervals for trace minerals and heavy metals in livers of cattle in wilderness areas in the Netherlands.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-24 DOI: 10.1177/10406387251325864

Deon van der Merwe, Yannique Jacobs, Saskia van der Drift, Ant Koopmans

{"title":"Reference intervals for trace minerals and heavy metals in livers of cattle in wilderness areas in the Netherlands.","authors":"Deon van der Merwe, Yannique Jacobs, Saskia van der Drift, Ant Koopmans","doi":"10.1177/10406387251325864","DOIUrl":"10.1177/10406387251325864","url":null,"abstract":"We examined the liver mineral concentrations of semi-wild cattle breeds (Highland, Galloway, Rode Geus) grazing in natural or near-natural flood-prone areas in the Netherlands. These breeds are employed as part of trophic rewilding initiatives aimed at maintaining biodiverse ecosystems through natural grazing patterns. Our primary goal was to derive RIs for liver mineral concentrations for these cattle populations. We collected and analyzed liver samples from 96 cattle from 14 sites in the Netherlands and used inductively coupled plasma mass spectrometry to measure concentrations of trace elements and heavy metals, including arsenic, cadmium, chromium, cobalt, copper, iron, lead, manganese, molybdenum, nickel, selenium, vanadium, and zinc. The statistical analysis included Pearson product moment correlations and k-means clustering to evaluate patterns within the data. The highest significant correlations were between liver cadmium concentrations and animal age, and between liver copper and selenium concentrations. Breed-related differences were found for liver cadmium and molybdenum concentrations, but we could not isolate breed-differences in forage selection from environmental variables such as soil composition. Liver copper levels in many animals were below thresholds typically used to indicate copper deficiency in farmed cattle.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251325864"},"PeriodicalIF":1.2,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Canine polyostotic B-cell lymphoma: a case with clinical, immunohistochemical, and flow cytometric characterization, and review of the literature.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-21 DOI: 10.1177/10406387251329020

Matthew Kornya, Connor Bryant, Brandon Lillie, Sebastien Sanz, Kristiina Ruotsalo, Dorothee Bienzle

{"title":"Canine polyostotic B-cell lymphoma: a case with clinical, immunohistochemical, and flow cytometric characterization, and review of the literature.","authors":"Matthew Kornya, Connor Bryant, Brandon Lillie, Sebastien Sanz, Kristiina Ruotsalo, Dorothee Bienzle","doi":"10.1177/10406387251329020","DOIUrl":"10.1177/10406387251329020","url":null,"abstract":"An 8-mo-old Mastiff-cross dog with bone pain and lytic-proliferative lesions in the radius, ulna, femur, vertebral spinous processes, and ribs, was diagnosed with lymphoma. The dog also had anemia and thrombocytopenia, and atypical circulating lymphocytes were identified as B cells by flow cytometry. Due to the multicentric, rapidly progressive disease, the dog was euthanized. Postmortem examination confirmed extensive bone replacement by lymphoma, and infiltration of lymph nodes, spleen, and liver. Histomorphology and immunohistochemistry showed a diffuse large B-cell lymphoma that was immunopositive for PAX5 and CD20, and immunonegative for CD3. Lymphoma of bone is rare in dogs and humans, and is most frequently reported in pediatric individuals. Including our case, 7 of 14 reported cases occurred in dogs <2-y-old, and all but 1 had polyostotic disease. Long bones, ribs, and vertebrae were affected most often, and the distal metaphyseal region was targeted in long bones. Visceral and nodal tissue infiltration was common, and all tumors had a diffuse architecture. Most dogs with polyostotic lymphoma were euthanized at the time of diagnosis, and survival was <6 wk in dogs that were treated with chemotherapy or surgery.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251329020"},"PeriodicalIF":1.2,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fast molecular tool for sexing Gibber Italicus, song thrush, and redwing birds.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-12 DOI: 10.1177/10406387251323555

Emanuele D'Anza, Sara Albarella, Ilaria Cascone, Mariagiulia Pugliano, Francesca Ciotola, Vincenzo Peretti

{"title":"A fast molecular tool for sexing Gibber Italicus, song thrush, and redwing birds.","authors":"Emanuele D'Anza, Sara Albarella, Ilaria Cascone, Mariagiulia Pugliano, Francesca Ciotola, Vincenzo Peretti","doi":"10.1177/10406387251323555","DOIUrl":"10.1177/10406387251323555","url":null,"abstract":"Gibber Italicus, song thrush (Turdus philomelus), and redwing (Turdus iliacus) are 3 bird species in which sexual dimorphism is not readily apparent. Therefore, molecular sexing is a valuable tool for breeding, selection, and conservation purposes. We compared DNA extraction by commercial kit and an alkaline method from feathers, then developed a molecular method for sexing these species using the P2/P8 and CHD1F/CHD1R primer pairs. Both protocols were successful in extracting DNA; the alkaline method is more cost-effective, whereas the commercial kit protocol is easier to standardize. All DNA samples were successfully amplified using both the P2/P8 and CHD1F/1R primer pairs. The use of the CHD1F/1R primer pair is notably advantageous as it produces fragments of different lengths of the sex chromosomes, making them easily distinguishable via electrophoresis. The sequence analysis of the amplicons obtained with the CHD1F/1R primer pair revealed specific single-nucleotide polymorphisms for song thrush and redwing, which could be used as markers to differentiate between the 2 species if required.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251323555"},"PeriodicalIF":1.2,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subcutaneous mast cell tumor with nodal metastasis in a serotine bat.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-12 DOI: 10.1177/10406387251324717

Gauthier Terrade, Valentine Muller, Gaël Berthévas, Hélène Huet, Edouard Reyes-Gomez

{"title":"Subcutaneous mast cell tumor with nodal metastasis in a serotine bat.","authors":"Gauthier Terrade, Valentine Muller, Gaël Berthévas, Hélène Huet, Edouard Reyes-Gomez","doi":"10.1177/10406387251324717","DOIUrl":"10.1177/10406387251324717","url":null,"abstract":"A subcutaneous tumor was identified in the lateral neck of a wild adult serotine bat (Eptesicus serotinus). Histologic examination revealed a non-encapsulated, poorly demarcated, densely cellular and infiltrative round-cell neoplasm, with cytonuclear atypia and a high mitotic count. Neoplastic cells had numerous Giemsa-positive metachromatic intracytoplasmic granules leading to a diagnosis of subcutaneous mast cell tumor (MCT). Immunohistochemical analysis demonstrated positive staining for KIT with a diffuse cytoplasmic pattern, confirming the mastocytic origin. The tumor had histologic features suggestive of malignant behavior, which was confirmed by the metastatic mast cells in the regional lymph node adjacent to the tumor. MCT has not been reported previously in a chiropteran species, to our knowledge. Based on this case, MCTs in this species have malignant potential, and their diagnosis may require additional stains and immunohistochemistry (IHC). Our results support further investigation regarding the utility and prognostic value of KIT IHC in bats in this context.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251324717"},"PeriodicalIF":1.2,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0