Journal of Veterinary Diagnostic Investigation最新文献

A fast molecular tool for sexing Gibber Italicus, song thrush, and redwing birds. 一个快速的分子工具，对意大利吉布，画眉和红翼鸟进行性别鉴定。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-12 DOI: 10.1177/10406387251323555

Emanuele D'Anza, Sara Albarella, Ilaria Cascone, Mariagiulia Pugliano, Francesca Ciotola, Vincenzo Peretti

{"title":"A fast molecular tool for sexing Gibber Italicus, song thrush, and redwing birds.","authors":"Emanuele D'Anza, Sara Albarella, Ilaria Cascone, Mariagiulia Pugliano, Francesca Ciotola, Vincenzo Peretti","doi":"10.1177/10406387251323555","DOIUrl":"10.1177/10406387251323555","url":null,"abstract":"Gibber Italicus, song thrush (Turdus philomelus), and redwing (Turdus iliacus) are 3 bird species in which sexual dimorphism is not readily apparent. Therefore, molecular sexing is a valuable tool for breeding, selection, and conservation purposes. We compared DNA extraction by commercial kit and an alkaline method from feathers, then developed a molecular method for sexing these species using the P2/P8 and CHD1F/CHD1R primer pairs. Both protocols were successful in extracting DNA; the alkaline method is more cost-effective, whereas the commercial kit protocol is easier to standardize. All DNA samples were successfully amplified using both the P2/P8 and CHD1F/1R primer pairs. The use of the CHD1F/1R primer pair is notably advantageous as it produces fragments of different lengths of the sex chromosomes, making them easily distinguishable via electrophoresis. The sequence analysis of the amplicons obtained with the CHD1F/1R primer pair revealed specific single-nucleotide polymorphisms for song thrush and redwing, which could be used as markers to differentiate between the 2 species if required.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"620-624"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904919/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of porcine circoviruses 2 and 3 in wild boar in Serbia. 猪圆环病毒2和3在塞尔维亚野猪中的流行率。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-11 DOI: 10.1177/10406387251325534

Sofija Šolaja, Dimitrije Glišić, Vesna Milićević

{"title":"Prevalence of porcine circoviruses 2 and 3 in wild boar in Serbia.","authors":"Sofija Šolaja, Dimitrije Glišić, Vesna Milićević","doi":"10.1177/10406387251325534","DOIUrl":"10.1177/10406387251325534","url":null,"abstract":"Porcine circovirus 2 (PCV2) and porcine circovirus 3 (PCV3) are prevalent pathogens that are economically important to the global swine industry. We investigated the prevalence of PCV2 and PCV3 in eastern Serbia by analyzing 166 wild boar samples with a real-time PCR assay. The cumulative prevalence of PCV2 and PCV3 was 72.7%, with true prevalence estimates of 59.2% for PCV2, 49.0% for PCV3, and 31.0% for coinfection. We found higher prevalence of PCV2 and PCV3 in the Južnobanatski district compared to previous reports, likely due to differences in detection methods and the inclusion of liver samples. Backyard farms, with free-ranging animals and natural breeding, dominate eastern Serbia, facilitating frequent interactions between wild and domestic pigs. The overlap between backyard and commercial farming practices, along with high wild boar densities and cross-border movements, likely facilitate viral transmission. These conditions, coupled with inadequate biosecurity measures, such as swill feeding and improper disposal of offal, amplify the risk for viral dissemination, with significant implications for spillover into domestic pig populations. Enhanced biosecurity and monitoring strategies are crucial to mitigate the risk of pathogen transmission between wild and domestic pig populations.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"674-678"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum. 勘误表。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-02 DOI: 10.1177/10406387251330690

引用次数: 0

Evaluating the stability of Tritrichomonas foetus in various media under simulated environmental conditions of shipment for RT-qPCR assays. 在模拟装运环境条件下评估毛滴虫胎儿在各种培养基中的稳定性，用于RT-qPCR检测。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-16 DOI: 10.1177/10406387251330297

SaraBeth Boggan, Ryan B Williams, Jennifer H Koziol

引用次数: 0

Myofibroblast differentiation in a cat with eosinophilic sclerosing fibroplasia involving mesenteric lymph nodes and liver. 猫嗜酸性硬化性纤维增生累及肠系膜淋巴结和肝脏的肌成纤维细胞分化。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-12 DOI: 10.1177/10406387251339813

Darian L Giannino, Mason C Jager, Hannah Brodlie, Rory C Chien

{"title":"Myofibroblast differentiation in a cat with eosinophilic sclerosing fibroplasia involving mesenteric lymph nodes and liver.","authors":"Darian L Giannino, Mason C Jager, Hannah Brodlie, Rory C Chien","doi":"10.1177/10406387251339813","DOIUrl":"10.1177/10406387251339813","url":null,"abstract":"Feline eosinophilic sclerosing fibroplasia (FESF) is a unique fibroproliferative disease of cats marked by eosinophilic inflammation and extensive tissue fibroplasia that affects the gastrointestinal tract predominantly. Biopsy specimens were examined from an 11-y-old, spayed female, domestic shorthair cat with a 1-2-mo history of vomiting and anorexia, abdominal lymphadenopathy, and multiple hepatic nodules. Microscopically, the liver nodules and enlarged mesenteric lymph node had profound eosinophilic inflammation and sclerosis characteristic of FESF; the stomach, duodenum, jejunum, and ileum were unremarkable. Immunohistochemistry (IHC) was performed to further characterize the cell populations. Increased intralesional mesenchymal cells, interpreted as reactive fibroblasts, were positive with antibodies against smooth muscle actin (SMA), indicating a myofibroblast phenotype. Abundant intralesional macrophages were ionized calcium-binding adapter molecule 1 (IBA1) immunolabeled. Dual IHC of SMA and IBA1 revealed several double-positive mesenchymal cells, suggesting macrophage-to-myofibroblast transition (MMT). Our findings underscore the important role of macrophages not only in chronic inflammation, but also in tissue repair and fibrosis. Our case was a unique presentation of FESF with primary liver and mesenteric lymph node involvement, and without overt gastrointestinal lesions. Additionally, to our knowledge, myofibroblast phenoconversion and MMT have not been reported previously in cats, giving new insights to the pathogenesis of this poorly understood disease entity.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"705-711"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075169/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144015512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dirofilaria immitis antigen detection in the urine of dogs with known and unknown infection status. 已知和未知感染状态犬尿液中免疫双丝虫抗原检测。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-01 DOI: 10.1177/10406387251327415

Andrew C Brown, Meriam N Saleh, J Mack Fudge, Mary B Nabity, Guilherme G Verocai

{"title":"Dirofilaria immitis antigen detection in the urine of dogs with known and unknown infection status.","authors":"Andrew C Brown, Meriam N Saleh, J Mack Fudge, Mary B Nabity, Guilherme G Verocai","doi":"10.1177/10406387251327415","DOIUrl":"10.1177/10406387251327415","url":null,"abstract":"Canine heartworm, Dirofilaria immitis, causes a potentially fatal, multisystemic disease in dogs. Diagnosis of heartworm disease relies on serologic antigen detection and microfilariae identification. Immune-complex dissociation (ICD) of serum or plasma by heat treatment increases detection. We assessed urine as a sample for heartworm antigen detection in dogs with known and unknown infection status using a commercial ELISA. Twenty-nine matching serum or plasma and urine samples were collected from 20 experimentally infected dogs to assess antigen detection in urine. Matching serum and urine samples of 267 dogs admitted to a spay/neuter clinic with unknown infection status were also screened. In experimentally infected dogs, antigenemia was detected in 86% of samples pre-ICD and 91% post-ICD; antigenuria was detected in 76% pre-ICD and 72% post-ICD; differences were not statistically significant. In clinic samples, antigenemia was detected in 7.9% of dogs pre-ICD and 12.4% post-ICD; antigenuria was detected in 11.6% of dogs pre-ICD and 6.4% post-ICD. In experimental infection samples, sensitivity (Se), specificity (Sp), and positive predictive value of antigenuria were high (85%, 100%, 100%, respectively). In clinic samples, Se and negative predictive value were high (95% and 92%, respectively). Our data confirm that heartworm antigen can be detected in the urine of dogs with both experimental heartworm infections and with unknown infection status. However, antigenuria had high Sp and low Se compared to matching serum of clinic dog samples and urine cannot be recommended for routine screening.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"584-591"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subcutaneous mast cell tumor with nodal metastasis in a serotine bat. 棕蝠皮下肥大细胞瘤伴淋巴结转移。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-12 DOI: 10.1177/10406387251324717

Gauthier Terrade, Valentine Muller, Gaël Berthévas, Hélène Huet, Edouard Reyes-Gomez

{"title":"Subcutaneous mast cell tumor with nodal metastasis in a serotine bat.","authors":"Gauthier Terrade, Valentine Muller, Gaël Berthévas, Hélène Huet, Edouard Reyes-Gomez","doi":"10.1177/10406387251324717","DOIUrl":"10.1177/10406387251324717","url":null,"abstract":"A subcutaneous tumor was identified in the lateral neck of a wild adult serotine bat (Eptesicus serotinus). Histologic examination revealed a non-encapsulated, poorly demarcated, densely cellular and infiltrative round-cell neoplasm, with cytonuclear atypia and a high mitotic count. Neoplastic cells had numerous Giemsa-positive metachromatic intracytoplasmic granules leading to a diagnosis of subcutaneous mast cell tumor (MCT). Immunohistochemical analysis demonstrated positive staining for KIT with a diffuse cytoplasmic pattern, confirming the mastocytic origin. The tumor had histologic features suggestive of malignant behavior, which was confirmed by the metastatic mast cells in the regional lymph node adjacent to the tumor. MCT has not been reported previously in a chiropteran species, to our knowledge. Based on this case, MCTs in this species have malignant potential, and their diagnosis may require additional stains and immunohistochemistry (IHC). Our results support further investigation regarding the utility and prognostic value of KIT IHC in bats in this context.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"630-634"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human hepatoma HepG2 cells are susceptible to infection by domestic cat hepadnavirus. 人肝癌HepG2细胞易受家猫肝病毒感染。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-12 DOI: 10.1177/10406387251339765

Sabrina Wahyu Wardhani, Tanit Kasantikul, Chutchai Piewbang, Somporn Techangamsuwan

{"title":"Human hepatoma HepG2 cells are susceptible to infection by domestic cat hepadnavirus.","authors":"Sabrina Wahyu Wardhani, Tanit Kasantikul, Chutchai Piewbang, Somporn Techangamsuwan","doi":"10.1177/10406387251339765","DOIUrl":"10.1177/10406387251339765","url":null,"abstract":"Domestic cat hepadnavirus (DCHBV; Hepadnaviridae, Orthohepadnavirus felisdomestici) is a hepadnavirus identified in domestic cats that is associated with liver conditions that resemble hepatitis B virus (HBV) infections in humans. Despite its significance for feline health, research on DCHBV has been hindered due to the absence of suitable in vitro models, particularly the lack of feline hepatocyte cell lines. We found that cells of the human-derived hepatoma cell line, HepG2, were susceptible to DCHBV infection. DCHBV DNA and RNA were detected from the infected HepG2 cells at 48 h postinfection; DCHBV core protein and viral particles were detected within the infected cells. No significant enhancement of viral infection was observed by the addition of dimethyl sulfoxide or dexamethasone to the culture medium. However, higher levels of viral uptake were noted at 4 d postinfection when using a concentrated viral inoculum. Although HepG2 cells supported initial DCHBV infection, viral load declined over time during serial infection experiments. The DCHBV infection of HepG2 cells is limited and declines over successive passages, potentially due to insufficient cellular factors for sustained viral replication. Our findings establish a foundation for further investigations of DCHBV biology and highlight the necessity for identifying alternative cell lines to improve long-term infection models.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"609-619"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutaneous and subcutaneous fibrolipomas in 236 dogs, 2017-2024: a retrospective case series and comparative review. 2017-2024年236只狗的皮肤和皮下纤维脂肪瘤：回顾性病例系列和比较回顾

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-01 DOI: 10.1177/10406387251328600

Ricardo E Mendes, Weerapong Laovechprasit, Sarah M Schneider, Daniel R Rissi

引用次数: 0

Pasteurella canis-associated bronchopneumonia in a 17-week-old Rottweiler and literature review. 17周龄罗威纳犬犬巴斯德氏菌相关性支气管肺炎及文献综述

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-11 DOI: 10.1177/10406387251325161

Frank V Pellegrini, Lorelei L Clarke

引用次数: 0