Journal of Veterinary Diagnostic Investigation最新文献

Fatal tracheal collapse and ossification in an osteopenic captive Indian blue peafowl in California.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-02 DOI: 10.1177/10406387251323870

Carlos Daniel Gornatti-Churria, Javier Asin, Aníbal G Armién, Veronica Nguyen, Simone T Stoute

引用次数: 0

Bovine abortions due to the rare Salmonella serovars Kingston and Kedougou.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-28 DOI: 10.1177/10406387251324261

Laurie Boucher, Pierre Hélie, Julie-Hélène Fairbrother, Sonia Chénier, Samuel Morin, Guillaume St-Jean

引用次数: 0

Laboratory assessment of recovery of porcine circovirus 2 and porcine reproductive and respiratory syndrome virus using 2 commercial hollow-fiber ultrafilters. 使用两种商用中空纤维超滤器回收猪圆环病毒 2 和猪繁殖与呼吸综合征病毒的实验室评估。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-28 DOI: 10.1177/10406387251322506

Aaron D Firnstahl, Gabrielle E Doughan, Sarah A Opelt, Rachel M Cook, Joseph A Heffron, Karen M Krueger, Mark A Borchardt, Locke A Karriker, Joel P Stokdyk, Tucker R Burch

{"title":"Laboratory assessment of recovery of porcine circovirus 2 and porcine reproductive and respiratory syndrome virus using 2 commercial hollow-fiber ultrafilters.","authors":"Aaron D Firnstahl, Gabrielle E Doughan, Sarah A Opelt, Rachel M Cook, Joseph A Heffron, Karen M Krueger, Mark A Borchardt, Locke A Karriker, Joel P Stokdyk, Tucker R Burch","doi":"10.1177/10406387251322506","DOIUrl":"10.1177/10406387251322506","url":null,"abstract":"Groundwater near swine farms is an uninvestigated reservoir for porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circoviruses (PCVs). Enteric microorganisms are often collected from groundwater via dead-end ultrafiltration, but recovery of PRRSV and PCV with this method has not been assessed. We recovered PRRSV2 and PCV2 by dead-end ultrafiltration followed by polyethylene glycol (PEG) precipitation, nucleic acid extraction, and reverse-transcription quantitative real-time PCR. We also compared 2 commercial hemodialysis ultrafilters (Asahi Kasei Rexeed-25A, Nipro Elisio-25H) and compared PRRSV2 recovery in these filters to other waterborne microorganisms. On average, 8 ± 1% of PRRSV2 was recovered by dead-end ultrafiltration and PEG precipitation, compared to 25 ± 6% for adenovirus 41. Full-process recovery of bacteria in the same filters was 5-15%; Cryptosporidium parvum recovery was 42 ± 12%. PCV2 was detected in 4 of 12 replicate filters, but low stock concentrations precluded quantitative recovery estimates. Elisio-25H ultrafilters performed similarly to Rexeed-25A filters for all organisms tested and is an effective replacement for the Rexeed-25A, which is no longer available in the United States. Our recovery of PRRSV2 and PCV2 by dead-end ultrafiltration in the laboratory suggests that PRRSV2 detection limits are as low as 3-50 genomic copies/L in sample volumes of 100-1,500 L. Based on quantitative microbial risk assessment, these concentrations are relevant to PRRSV2 infection rates in the U.S. swine herd.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251322506"},"PeriodicalIF":1.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cecal transection with transanal evisceration in a goat.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-28 DOI: 10.1177/10406387251324514

Kristen L Shekelle, Fausto Bellezzo, Mark J Trautmann, Laura K Bryan

{"title":"Cecal transection with transanal evisceration in a goat.","authors":"Kristen L Shekelle, Fausto Bellezzo, Mark J Trautmann, Laura K Bryan","doi":"10.1177/10406387251324514","DOIUrl":"10.1177/10406387251324514","url":null,"abstract":"A 16-mo-old, pregnant, Nigerian Dwarf doe was presented to the veterinary hospital after being struck by a farm truck. A piece of tissue was found on the ground next to the goat after impact. The doe was painful on abdominal and perineal palpation, but abdominal radiographs and ultrasound did not reveal fractures or soft tissue herniations. The accompanying piece of tissue was a blind-ended sac covered with fecal material, most suggestive of the cecal apex. The goat declined to lateral recumbency, and due to the poor prognosis, the owners elected euthanasia. Postmortem examination identified free feces throughout the abdominal cavity, a complete, circumferential laceration through the mid-body of the cecum with an absent apex, and a full-thickness rectal tear at the anal orifice. Histologic examination of the accompanying piece of tissue was confirmatory of the cecal apex, and the anorectal tear had acute hemorrhage most consistent with trauma. Our report highlights the unique case presentation of traumatic cecal transection with transanal evisceration through an anorectal tear, a combination of injuries not previously reported in people or animals, to our knowledge. Pregnancy is a predisposing factor to rectal injuries in humans and veterinary species.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251324514"},"PeriodicalIF":1.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproductive loss attributed to Lactococcus petauri infection in a black-and-white ruffed lemur. 黑白狐猴因感染 Petauri 乳球菌而丧失生殖能力。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-28 DOI: 10.1177/10406387251323565

Agustín Rebollada-Merino, Ana Isabel Vela, Rocío Canales, Umberto Romani-Cremaschi, María Ugarte-Ruiz, Arantxa Buendía, Marta Pérez-Sancho, Lucas Domínguez, José Francisco Fernández-Garayzabal, Antonio Rodríguez-Bertos

{"title":"Reproductive loss attributed to Lactococcus petauri infection in a black-and-white ruffed lemur.","authors":"Agustín Rebollada-Merino, Ana Isabel Vela, Rocío Canales, Umberto Romani-Cremaschi, María Ugarte-Ruiz, Arantxa Buendía, Marta Pérez-Sancho, Lucas Domínguez, José Francisco Fernández-Garayzabal, Antonio Rodríguez-Bertos","doi":"10.1177/10406387251323565","DOIUrl":"10.1177/10406387251323565","url":null,"abstract":"Lactococci have been associated with fetal and neonatal infections in humans and cattle. Here we describe a case of reproductive loss attributed to Lactococcus petauri in a lemur. A full-term black-and-white ruffed lemur (Varecia variegata) was found dead in the indoor area of a zoologic exhibit. Classification as a late-term abortion or stillbirth was unclear as the precise gestational time was unknown. A medical checkup of the dam revealed fever and neutrophilic leukocytosis; recovery followed treatment with enrofloxacin. The main histologic findings were placental edema and hemorrhage, hepatic necrosis, desquamated amniotic epithelial cells in alveoli, and subendocardial and myocardial hemorrhages. Tissue Gram stain revealed abundant gram-positive cocci arranged in short chains in the placenta and liver. Toxoplasma gondii was not detected by immunohistochemistry. Bacterial isolates from the placenta and fetal liver were identified as Lactococcus garvieae by MALDI-TOF MS. However, the isolates were found to be L. petauri by determining their in-silico DNA-DNA hybridization and average nucleotide identity values using pairwise comparisons of their whole-genome sequences and the genomes of the type strains. The antimicrobial susceptibility of isolates by the disk diffusion method revealed resistance to tylosin, gentamicin, apramycin, neomycin, amikacin, ampicillin, and florfenicol. We attributed the reproductive loss in this lemur to placental and fetal infection by L. petauri.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251323565"},"PeriodicalIF":1.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardiac osteochondrolipoma in a cheetah.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-28 DOI: 10.1177/10406387251322763

Jeong-Seop Oh, Na-Young Lee, Eun Jung, Ki-Yong Shin, Dong-Hee Chung, Dae-Yong Kim, Soong-Hee Youn

引用次数: 0

Detection of equid alphaherpesvirus 1 in serum samples collected from infected horses.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-28 DOI: 10.1177/10406387251323272

Koji Tsujimura, Hiroshi Bannai, Yoshinori Kambayashi, Manabu Nemoto, Minoru Ohta

{"title":"Detection of equid alphaherpesvirus 1 in serum samples collected from infected horses.","authors":"Koji Tsujimura, Hiroshi Bannai, Yoshinori Kambayashi, Manabu Nemoto, Minoru Ohta","doi":"10.1177/10406387251323272","DOIUrl":"10.1177/10406387251323272","url":null,"abstract":"Equid alphaherpesvirus 1 (EqAHV1; Orthoherpesviridae, Varicellovirus equidalpha1) spreads by viremia to susceptible organs. Because EqAHV1 circulates in the bloodstream in a cell-associated manner, serum samples are not considered valuable for detecting EqAHV1 and have therefore not been tested by highly sensitive detection methods such as real-time PCR (rtPCR). We investigated whether EqAHV1 could be detected by this method in equine serum samples. We performed rtPCR on archived sera and peripheral blood mononuclear cells (PBMCs) collected from 3 horses experimentally inoculated with EqAHV1. Acute-phase field sera from 40 febrile horses, including 11 positive for EqAHV1 on antibody ELISA, were also tested by both standard rtPCR and direct rtPCR without nucleic acid purification. EqAHV1 was detected by standard rtPCR in the PBMCs of the experimentally infected horses for 3-6 d and in the serum of these horses for 5-7 d. Six of the 11 ELISA-positive acute-phase field sera were positive on standard rtPCR, whereas the remaining were negative. All 6 of these samples were positive on direct rtPCR without nucleic acid purification. These results suggest that serum samples can be used to detect EqAHV1; however, false-negatives may result from low viral gene copy numbers.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251323272"},"PeriodicalIF":1.2,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871572/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enterotoxemia in a 2-day-old lamb produced by a Clostridium perfringens type D lambda toxin-positive strain.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-27 DOI: 10.1177/10406387251320943

Kaylin Moctezuma, Hernando D Acevedo, Eileen E Henderson, Javier Asin, John M Adaska, Francisco A Uzal

{"title":"Enterotoxemia in a 2-day-old lamb produced by a Clostridium perfringens type D lambda toxin-positive strain.","authors":"Kaylin Moctezuma, Hernando D Acevedo, Eileen E Henderson, Javier Asin, John M Adaska, Francisco A Uzal","doi":"10.1177/10406387251320943","DOIUrl":"10.1177/10406387251320943","url":null,"abstract":"Clostridium perfringens type D is a gram-positive bacterium that causes enterotoxemia in sheep, goats, and, less frequently, other animals. This microorganism encodes 2 major toxins, alpha (CPA) and epsilon (ETX). Enterotoxemia occurs when epsilon prototoxin (pETX) is produced in the intestine and is activated by one or more proteases before being absorbed into the general circulation. Traditionally, it was believed that neonatal animals were not susceptible to type D enterotoxemia due to the trypsin-inhibitory action of colostrum in the intestinal tract and the lack of protease activation of pETX, although cases of enterotoxemia have been reported in 2 neonatal goat kids. A 2-d-old lamb, with a history of frailty, hunched posture, shallow breathing, and diarrhea followed by death, was submitted for postmortem examination and diagnostic workup. Autopsy revealed hydropericardium, pulmonary edema, and congested intestines. Histologically, there was pulmonary congestion and edema, and intestinal congestion. C. perfringens type D carrying the gene for lambda toxin was isolated from the small intestine and colon. Both CPA and ETX were detected in the small intestine and colon contents. We speculate that lambda toxin (a protease) activated pETX, and that ETX, in turn, produced enterotoxemia in this lamb.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251320943"},"PeriodicalIF":1.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Mycoplasmopsis (Mycoplasma) bovis in formalin-fixed, paraffin-embedded bovine lung sections by immunohistochemistry and in situ hybridization.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-24 DOI: 10.1177/10406387251322463

Matthew Brown, Zackary Laney, Laila Tabatabai, Anna Hassebroek, Kevin Lahmers, Tessa LeCuyer, Francisco A Uzal, Francisco R Carvallo

{"title":"Detection of Mycoplasmopsis (Mycoplasma) bovis in formalin-fixed, paraffin-embedded bovine lung sections by immunohistochemistry and in situ hybridization.","authors":"Matthew Brown, Zackary Laney, Laila Tabatabai, Anna Hassebroek, Kevin Lahmers, Tessa LeCuyer, Francisco A Uzal, Francisco R Carvallo","doi":"10.1177/10406387251322463","DOIUrl":"10.1177/10406387251322463","url":null,"abstract":"The bovine respiratory disease complex (BRDC) is a multifactorial disease of economic importance in cattle involving viral and bacterial agents and several environmental and host-associated predisposing factors. Mycoplasmopsis (Mycoplasma) bovis is frequently detected in BRDC cases, but the role of this bacterium in the pathogenesis of BRDC is not completely understood. We explored the utility of routine histopathology and compared immunohistochemistry (IHC) and in situ hybridization (ISH) in pneumonic bovine lung tissue samples for the detection of M. bovis infection. Samples were analyzed for M. bovis using mycoplasma bacterial culture (screening test), H&E staining, IHC, and ISH. We found that \"compatible histologic lesions\" are not entirely predictive of the presence of M. bovis on culture, IHC, or ISH, and also that there was no statistical difference between IHC and ISH for detecting M. bovis. We conclude that IHC and ISH can be used interchangeably to detect M. bovis infections in pneumonic bovine lung.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251322463"},"PeriodicalIF":1.2,"publicationDate":"2025-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11851585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High prevalence and broad geographic distribution of urine shedding of bovine polyomavirus 1 (Epsilonpolyomavirus bovis) by cattle in Uruguay. 乌拉圭牛尿中牛多瘤病毒 1（Epsilonpolyomavirus bovis）的高流行率和广泛地理分布。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-02-20 DOI: 10.1177/10406387251321421

Matías Castells, Leticia Zarantonelli, Caroline da Silva Silveira, Rodney Colina, Federico Giannitti

{"title":"High prevalence and broad geographic distribution of urine shedding of bovine polyomavirus 1 (Epsilonpolyomavirus bovis) by cattle in Uruguay.","authors":"Matías Castells, Leticia Zarantonelli, Caroline da Silva Silveira, Rodney Colina, Federico Giannitti","doi":"10.1177/10406387251321421","DOIUrl":"10.1177/10406387251321421","url":null,"abstract":"In 2021, bovine polyomavirus 1 (BoPyV1; Polyomaviridae, Epsilonpolyomavirus bovis) was associated with nephritis in an aborted bovine fetus in Uruguay, with renal lesions resembling those typical of polyomavirus-associated nephropathy of humans. Given that little is known about the epidemiology of BoPyV1 infection in cattle, we screened for BoPyV1 in urine samples collected in 2015-2017 from beef and dairy herds from 12 of the 19 departments in Uruguay. We tested for BoPyV1 by PCR in 156 urine pools and 249 individual urine samples from 42 herds. We detected BoPyV1 in 33 of 42 (79%) farms across 100% of the departments studied, in similar proportions in beef (22 of 30; 73%) and dairy (11 of 12; 92%) herds. At the animal level, BoPyV1 was detected in 80 of 249 (32%) animals; this frequency was significantly higher in dairy (51 of 119; 43%) than beef (29 of 130; 22%) cattle, and in cows (36 of 81; 44%) than heifers (32 of 121; 26%). BoPyV1 strains circulating in Uruguay have a high degree (98.7-100%) of sequence identity at the major capsid protein VP1, which is slightly lower (96.2-99.7%) than for strains from other countries. We conclude that shedding of BoPyV1 in the urine of dairy and beef cattle is prevalent and geographically widespread in Uruguay.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251321421"},"PeriodicalIF":1.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0