Journal of Veterinary Diagnostic Investigation最新文献

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Congenital vascular malformations in the intestine of 2 neonatal goat kids. 山羊新生儿肠道先天性血管畸形2例。
IF 1.1 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2026-05-01 Epub Date: 2026-01-26 DOI: 10.1177/10406387251385980
Agustín Rebollada-Merino, Javier Asín, Eileen Henderson, Francisco A Uzal
{"title":"Congenital vascular malformations in the intestine of 2 neonatal goat kids.","authors":"Agustín Rebollada-Merino, Javier Asín, Eileen Henderson, Francisco A Uzal","doi":"10.1177/10406387251385980","DOIUrl":"10.1177/10406387251385980","url":null,"abstract":"<p><p>Disorders of the intestinal vasculature in animals include arteriovenous malformations, telangiectasia (ectasia, angiodysplasia), hamartomas, and neoplasia (hemangiomas, hemangiosarcomas). Two, 2-d-old, female Nigerian dwarf goat kids born to different dams on the same farm had severe and progressive abdominal distension and were euthanized. The autopsies revealed segmental circumferential dark-red areas that caused focal constriction of the mid-jejunum. The constriction led to obstruction of the intestinal lumen and proximal dilation of the jejunum and duodenum in both cases. <i>Clostridium perfringens</i> type D enterotoxemia was also diagnosed in one case. Histologic examination in both cases revealed a focal, moderately well-demarcated proliferation of medium- to small-caliber vascular channels with luminal erythrocytes, lined by morphologically normal endothelial cells, that expanded the tunica muscularis and serosa of the jejunum and compressed the mucosa and the lumen. The congenital nature of these lesions favors a diagnosis of vascular hamartomas; however, the histologic findings were indistinguishable from those of intestinal hemangiomas reported in young humans, horses, a pig, and a dog. The age of the affected goats, the location of the lesions in the same segment of the jejunum, and the presence of only one buck on the farm were suggestive of a genetic origin for these lesions. It is speculated that intestinal obstruction was the predisposing factor for <i>C. perfringens</i> type D enterotoxemia in one of the goats.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"487-490"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12846903/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146052704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seronegative disseminated neosporosis with fatal outcome in an adult dog following prednisone therapy. 经强的松治疗的成年犬血清阴性播散性新孢子病致死性结局。
IF 1.1 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2026-05-01 Epub Date: 2026-01-29 DOI: 10.1177/10406387261417402
Constanza L Rodriguez, Kayla Fowler, Kevin Lahmers, Michelle Todd, Rell L Parker, Francisco A Uzal, Javier Asin, Francisco R Carvallo-Chaigneau
{"title":"Seronegative disseminated neosporosis with fatal outcome in an adult dog following prednisone therapy.","authors":"Constanza L Rodriguez, Kayla Fowler, Kevin Lahmers, Michelle Todd, Rell L Parker, Francisco A Uzal, Javier Asin, Francisco R Carvallo-Chaigneau","doi":"10.1177/10406387261417402","DOIUrl":"10.1177/10406387261417402","url":null,"abstract":"<p><p>A 4-y-old, male English Mastiff with a history of inflammatory neuromuscular disease developed progressive tetraparesis, ataxia, and severe temporal muscle atrophy, culminating in non-ambulatory status and euthanasia. The autopsy revealed diffuse muscle atrophy and pale pulmonary nodules. Histologically, polyphasic myositis was evident, with apicomplexan organisms within skeletal muscle myofibers. Encephalomyelitis, myocarditis, and hepatitis with protozoal cysts and tachyzoites also were observed. Immunohistochemistry of brain tissue was strongly positive for <i>Neospora caninum</i> and <i>Toxoplasma gondii</i>, but PCR testing confirmed <i>N. caninum</i> and excluded <i>T. gondii</i>, establishing a diagnosis of disseminated neosporosis. Despite extensive tissue involvement, <i>N. caninum</i> antibody titers were below the diagnostic cutoff. Our case highlights that non-positive serologic results do not exclude neosporosis in clinically compatible cases, particularly in dogs undergoing corticoid therapy, and underscores the diagnostic value of histopathology with confirmatory molecular testing for definitive diagnosis.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"555-558"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12858385/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146086142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Congenital nerve sheath tumor in a dog. 狗的先天性神经鞘肿瘤。
IF 1.1 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2026-05-01 Epub Date: 2026-01-22 DOI: 10.1177/10406387251415422
Jesse Cole, Paula A Schaffer, Natalie M Kirk
{"title":"Congenital nerve sheath tumor in a dog.","authors":"Jesse Cole, Paula A Schaffer, Natalie M Kirk","doi":"10.1177/10406387251415422","DOIUrl":"10.1177/10406387251415422","url":null,"abstract":"<p><p>A 5-wk-old, 10.6-kg, intact female Leonberger dog was presented for evaluation of a mass on the left ventrolateral thorax that had been present since birth. A biopsy of the mass revealed an invasive, unencapsulated spindle-cell population arranged in bundles and concentric whorls (pseudo-onion bulb formations) with multifocal melanocytic differentiation. Neoplastic cells in pseudo-onion bulbs immunolabeled strongly for glial acidic fibrillary protein and PGP9.5 and moderately for S100 and Sox10. The supporting matrix had strong immunolabeling for laminin. Cells had multifocal immunolabeling for NeuN, melan A, and PNL2. Collectively, these histopathologic characteristics support a diagnosis of congenital nerve sheath tumor, which is rarely described in dogs.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"500-504"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12830347/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection of Treponema species associated with bovine digital dermatitis in cattle using novel real-time PCR assays. 新型实时荧光定量PCR检测牛数字性皮炎相关密螺旋体。
IF 1.1 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2026-05-01 Epub Date: 2026-03-04 DOI: 10.1177/10406387261424230
Laura Tassera, Isabelle Brodard, Jörg Jores, Peter Kuhnert
{"title":"Detection of <i>Treponema</i> species associated with bovine digital dermatitis in cattle using novel real-time PCR assays.","authors":"Laura Tassera, Isabelle Brodard, Jörg Jores, Peter Kuhnert","doi":"10.1177/10406387261424230","DOIUrl":"10.1177/10406387261424230","url":null,"abstract":"<p><p>Three <i>Treponema</i> sp.-<i>T. medium</i>, <i>T. pedis</i>, and <i>T. phagedenis-</i>are associated with bovine digital dermatitis (BDD). This painful infectious disease has been reported worldwide, causing economic and animal welfare problems. Although detection by real-time PCR (rtPCR) is the method of choice, published assays differ in specificity and sensitivity. We multiplexed target regions of 2 published rtPCR assays and slightly optimized another one to compare their performance. We then used the 2 novel TaqMan rtPCR assays to determine the prevalence of the 3 <i>Treponema</i> sp. in a random sample of hind feet from 137 animals collected at slaughterhouses. An overall prevalence at the animal level of 31.4% was found for <i>T. phagedenis</i> and 10.2% for <i>T. medium</i>, with the latter always being detected together with <i>T. phagedenis</i>. We did not detect <i>T. pedis</i> in our sample set. Typical lesions of BDD were present in 16.2% of animals. Of the animals with BDD lesions, 52.6% were positive for any of the 3 <i>Treponema</i> sp. A significantly lower positive rate for <i>Treponema</i> sp. was found in animals without lesions, underscoring the role of these organisms in the development of BDD. Our study offers insight into the prevalence of BDD-associated <i>Treponema</i> sp. in cattle as well as a tool for routine diagnosis and future control of the disease.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"451-457"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12962984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147355645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Congenital oropharyngeal teratoma in a neonatal goat and brief review of extragonadal teratomas in animals. 新生山羊的先天性口咽畸胎瘤和动物角外畸胎瘤的简要回顾。
IF 1.1 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2026-05-01 Epub Date: 2026-01-29 DOI: 10.1177/10406387251410495
Sang-Joon Lee, Pedro Alejandro Triana Garcia, Eunju April Choi
{"title":"Congenital oropharyngeal teratoma in a neonatal goat and brief review of extragonadal teratomas in animals.","authors":"Sang-Joon Lee, Pedro Alejandro Triana Garcia, Eunju April Choi","doi":"10.1177/10406387251410495","DOIUrl":"10.1177/10406387251410495","url":null,"abstract":"<p><p>Teratomas originate from pluripotent germ cells and differentiate into the 3 germ cell layers: endoderm, mesoderm, and ectoderm. Hence, these tumors arise most often in the gonads. Extragonadal teratomas are rare in veterinary medicine. Congenital oropharyngeal teratoma, also known as epignathus, is a neoplasm that has been reported in humans and a few veterinary species. We describe the clinical, gross, cytologic, and histopathologic features of an oropharyngeal teratoma in a neonatal Boer × Nigerian Dwarf goat that died within 4 h of birth, and briefly review extragonadal teratomas in veterinary species.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"540-544"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12858381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146086179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pulmonary pneumocystosis in a captive kinkajou with molecular evidence of a novel Pneumocystis lineage. 圈养蜜熊肺部肺囊虫病与一种新型肺囊虫谱系的分子证据。
IF 1.1 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2026-05-01 Epub Date: 2026-01-22 DOI: 10.1177/10406387251413289
Pablo Díaz-Santana, Alejandro Suárez-Bonnet, Javier Déniz-Marrero, Francisco J Salguero, Bernat Martí-Garcia, Vincente Friaza, Enrique J Calderón, Sai Fingerhood
{"title":"Pulmonary pneumocystosis in a captive kinkajou with molecular evidence of a novel <i>Pneumocystis</i> lineage.","authors":"Pablo Díaz-Santana, Alejandro Suárez-Bonnet, Javier Déniz-Marrero, Francisco J Salguero, Bernat Martí-Garcia, Vincente Friaza, Enrique J Calderón, Sai Fingerhood","doi":"10.1177/10406387251413289","DOIUrl":"10.1177/10406387251413289","url":null,"abstract":"<p><p>An 11-mo-old, intact male captive kinkajou (<i>Potos flavus</i>) was submitted for postmortem investigation because of emaciation and hindlimb overgrooming. Histologically, alveolar airspaces were filled with fungal structures that were morphologically and histochemically consistent with <i>Pneumocystis</i> spp. PCR of pulmonary tissue was negative for canine distemper virus and positive for <i>Pneumocystis</i> spp. Molecular testing yielded amplification of the <i>Pneumocystis</i> spp. mitochondrial large-subunit rRNA (mtLSU rRNA, 510 bp) and the small-subunit rRNA (mtSSU rRNA, 565 bp). Phylogenetic analysis suggested a potentially novel <i>Pneumocystis</i> lineage associated with <i>P. flavus</i>. Additional nuclear loci are required to confirm its taxonomic status. Gastric and colonic histologic findings included concurrent candidiasis and colonic nematodosis. An underlying immunosuppressive disease was suspected. Further investigation is required to clarify the role of kinkajous in the ecology of fungal pathogens and the causes of immunosuppression in this species, particularly in the context of human-wildlife interactions. Enhanced surveillance and interdisciplinary collaboration are essential to evaluate potential zoonotic risks and inform conservation and public health strategies.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"550-554"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12830342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metastatic potential of ovine pulmonary adenocarcinoma: a comprehensive assessment of the draining pulmonary lymph nodes. 羊肺腺癌的转移潜能:对引流肺淋巴结的综合评估。
IF 1.1 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2026-05-01 Epub Date: 2026-01-23 DOI: 10.1177/10406387251409774
Dragoș Hodor, Andrada Negoescu, Romelia Pop, Roxana Popa, Cristina Borfalău, Alina-Diana Hașaș, Cristina Daniela Cazan, Jennifer Savage, Chris Cousens, Kobi Aharoni, Monika Hilbe, Giuliana Rosato, Iris Dumitru, Monika Olech, Vlad I Bocăneț, Cornel Cătoi, Marian Taulescu, Corina Toma
{"title":"Metastatic potential of ovine pulmonary adenocarcinoma: a comprehensive assessment of the draining pulmonary lymph nodes.","authors":"Dragoș Hodor, Andrada Negoescu, Romelia Pop, Roxana Popa, Cristina Borfalău, Alina-Diana Hașaș, Cristina Daniela Cazan, Jennifer Savage, Chris Cousens, Kobi Aharoni, Monika Hilbe, Giuliana Rosato, Iris Dumitru, Monika Olech, Vlad I Bocăneț, Cornel Cătoi, Marian Taulescu, Corina Toma","doi":"10.1177/10406387251409774","DOIUrl":"10.1177/10406387251409774","url":null,"abstract":"<p><p>Ovine pulmonary adenocarcinoma (OPA), caused by Jaagsiekte sheep retrovirus (JSRV; family <i>Retroviridae</i>, taxon species <i>Betaretrovirus ovijaa</i>), is a viral oncogenic lung disease in sheep. Its metastatic potential remains under-evaluated. We investigated macrometastases (MACs), micrometastases (MICs), and isolated tumor cells (ITCs) in regional draining lymph nodes (DLNs) using histopathology and immunohistochemistry (IHC). Samples from 41 lung tumors and their regional DLNs were obtained from slaughtered Țurcană sheep. Histologically, all cases were diagnosed as OPAs. The classical or mixed OPA was observed in 37 of 41 (90%) cases; the remaining tumors were the atypical form. In 10 cases, myxoid growths were also detected. For IHC, anti-multicytokeratin, thyroid transcription factor 1, and JSRV antibodies were used to detect metastatic cells within DLNs. Neoplastic cells were identified in 16 of 41 (39%) DLNs, including 2 MAC, 7 MIC, and 7 ITC cases. Lung tumors >7 cm were significantly associated with lymph node metastasis (<i>p</i> < 0.05). A random forest model incorporating tumor volume, necrosis, mitotic count, and Ki67 index achieved the best performance (AUC = 0.70; accuracy = 62.5%; F1 = 0.57) for metastasis prediction. A benign epithelial inclusion was found within a DLN in one case, which has not been reported previously, to our knowledge. We found that OPA has a higher metastatic potential than previously recognized, particularly in larger tumors. Multivariate analysis, including additional tumor markers, likely would improve metastasis prediction. Our findings advance our understanding of OPA progression and its relevance as a comparative model for human lung adenocarcinoma.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"419-430"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12830353/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146030279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of enzootic nasal tumor virus 1-associated enzootic nasal adenocarcinoma in sheep and development of a tumor-derived organoid model. 绵羊地方性鼻肿瘤病毒1相关地方性鼻腺癌的特征及肿瘤衍生类器官模型的建立
IF 1.1 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2026-05-01 Epub Date: 2026-01-29 DOI: 10.1177/10406387251410477
Viju V Pillai, Shyamkumar Ts, Sunil K Mor
{"title":"Characterization of enzootic nasal tumor virus 1-associated enzootic nasal adenocarcinoma in sheep and development of a tumor-derived organoid model.","authors":"Viju V Pillai, Shyamkumar Ts, Sunil K Mor","doi":"10.1177/10406387251410477","DOIUrl":"10.1177/10406387251410477","url":null,"abstract":"<p><p>Enzootic nasal adenocarcinoma (ENA) is a contagious neoplasm of the ethmoid turbinate mucosa in sheep, caused by enzootic nasal tumor virus 1 (ENTV1; family <i>Retroviridae</i>, unclassified <i>Betaretrovirus</i>). We report an outbreak of ENTV1-associated ENA in a sheep flock in South Dakota, USA. Affected animals had dyspnea, unilateral nasal discharge, and progressive weight loss. Postmortem examination revealed unilateral nasal masses that were diagnosed histologically as invasive nasal adenocarcinoma. PCR amplification followed by Sanger sequencing of the <i>gag</i> and <i>env</i> gene regions confirmed the presence of ENTV1. Our proviral genome assembly via next-generation sequencing is only the second ENTV1 sequence submitted to GenBank from the United States. Our isolate clustered within the ENTV1 clade and was closely related to the reported U.S. and Canadian isolates, indicating a shared evolutionary lineage. To further investigate tumor biology, we established 3-dimensional organoids derived from the nasal adenocarcinoma, which maintained the histologic features of the primary tumor and tested positive for ENTV1. These organoids also had an invasive phenotype, demonstrating their potential utility as a novel in vitro model for studying ENA pathogenesis and evaluating therapeutic interventions.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"431-436"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12858389/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146086134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation of an unusual morbidity event in lactating dairy cows in the Texas Panhandle and eastern New Mexico. 在德克萨斯州狭长地带和新墨西哥州东部的泌乳奶牛的不寻常发病率事件的调查。
IF 1.1 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2026-05-01 Epub Date: 2026-02-17 DOI: 10.1177/10406387251413567
Alexis C Thompson, Brandon J Dominguez, Terry S Hensley, Carlos A Rodriguez, Amy K Swinford, Katie E Kleinhenz, Erin E Edwards, Gabriel Gomez, Kiril M Dimitrov
{"title":"Investigation of an unusual morbidity event in lactating dairy cows in the Texas Panhandle and eastern New Mexico.","authors":"Alexis C Thompson, Brandon J Dominguez, Terry S Hensley, Carlos A Rodriguez, Amy K Swinford, Katie E Kleinhenz, Erin E Edwards, Gabriel Gomez, Kiril M Dimitrov","doi":"10.1177/10406387251413567","DOIUrl":"10.1177/10406387251413567","url":null,"abstract":"<p><p>In March and April 2024, an unusual morbidity event (UME) in the Texas Panhandle and eastern New Mexico was reported to the Texas A&M Veterinary Medical Diagnostic Laboratory. In total, 73 of 158 (46.2%) grade A dairy farms reported decreased milk production, reduced feed intake, and/or abnormal milk consistency. To identify potential etiologies, 22 affected operations submitted samples from 250 clinically affected cows and 9 operations submitted samples from 69 unaffected cows. Initial diagnostic efforts found no consistent causative factors between operations. Nine peridomestic birds and 10 domestic cats from affected premises tested positive for the highly pathogenic avian influenza virus (HPAIV) H5N1 subtype. On March 25, 2024, the United States Department of Agriculture confirmed HPAIV in unpasteurized milk from dairy cows in the region. Feces, serum, and whole blood from affected cows tested negative for H5N1 HPAIV. Milk from affected cows had 6.0 (95% CI [3.4, 10.8]) odds of being positive compared with unaffected cows. Our initial investigation ruled out many common pathogens allowing for the exploration of novel etiologies and facilitated testing of samples from affected premises through the National Animal Health Laboratory Network. When investigating an UME, clinical signs and histories from affected individuals should dictate sample and test selection; however, it is imperative that samples from unaffected individuals be submitted for comparison when determining a potential etiology.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"513-518"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12916330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146213785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Discovery of a novel bandavirus using metagenomic sequencing in a retrospective analysis of an unresolved 2020 mortality event involving wild black vultures in the northeastern United States. 在对涉及美国东北部野生黑秃鹫的未解决的2020年死亡事件的回顾性分析中,使用宏基因组测序发现了一种新型班达病毒。
IF 1.1 3区 农林科学
Journal of Veterinary Diagnostic Investigation Pub Date : 2026-05-01 Epub Date: 2026-02-13 DOI: 10.1177/10406387261418632
Lusajo Mwakibete, Axel O G Hoarau, Vida Ahyong, Eric Waltari, Susan J Bender, Sherrill Davison, Kevin D Niedringhaus, Michelle L Gibison, Roderick B Gagne, Erica A Miller, Lisa A Murphy, Amy L Kistler, Cristina M Tato
{"title":"Discovery of a novel bandavirus using metagenomic sequencing in a retrospective analysis of an unresolved 2020 mortality event involving wild black vultures in the northeastern United States.","authors":"Lusajo Mwakibete, Axel O G Hoarau, Vida Ahyong, Eric Waltari, Susan J Bender, Sherrill Davison, Kevin D Niedringhaus, Michelle L Gibison, Roderick B Gagne, Erica A Miller, Lisa A Murphy, Amy L Kistler, Cristina M Tato","doi":"10.1177/10406387261418632","DOIUrl":"10.1177/10406387261418632","url":null,"abstract":"<p><p>Investigations of wildlife diseases and mortality events can sometimes lead to inconclusive results because of limitations in testing combined with an ever-increasing number of emerging viruses. The use of tools such as unbiased metagenomic next-generation sequencing (mNGS) can facilitate the identification of causative agents when conventional investigation methods fail. We performed a retrospective mNGS analysis on RNA isolated from postmortem samples collected during a mortality event in free-ranging, wild black vultures (<i>Coragyps atratus</i>) that occurred in eastern Pennsylvania and western New Jersey in 2020. We describe the discovery and identification of a novel species of bandavirus (family <i>Phenuiviridae</i>) in case specimens from this die-off, as well as some of the associated pathology findings. The <i>Bandavirus</i> genus comprises tickborne viral species that have been reported across 5 continents. These viruses have been implicated in outbreaks in a variety of mammalian hosts, including humans, and in avian species, making them important potential sources of zoonotic spillover events. Genomic and phylogenetic analyses of the bandavirus that we detected indicate that its closest relative is Hunter Island virus, a bandavirus previously implicated in albatross mortality events off the coast of Tasmania, Australia. Follow-up PCR testing of samples from 16 additional vultures from the same cohort indicate that this new bandavirus was the likely cause of death.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"399-410"},"PeriodicalIF":1.1,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12904811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146180789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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