Journal of Veterinary Diagnostic Investigation最新文献_第2页

Subcutaneous mast cell tumor with nodal metastasis in a serotine bat.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-12 DOI: 10.1177/10406387251324717

Gauthier Terrade, Valentine Muller, Gaël Berthévas, Hélène Huet, Edouard Reyes-Gomez

{"title":"Subcutaneous mast cell tumor with nodal metastasis in a serotine bat.","authors":"Gauthier Terrade, Valentine Muller, Gaël Berthévas, Hélène Huet, Edouard Reyes-Gomez","doi":"10.1177/10406387251324717","DOIUrl":"10.1177/10406387251324717","url":null,"abstract":"A subcutaneous tumor was identified in the lateral neck of a wild adult serotine bat (Eptesicus serotinus). Histologic examination revealed a non-encapsulated, poorly demarcated, densely cellular and infiltrative round-cell neoplasm, with cytonuclear atypia and a high mitotic count. Neoplastic cells had numerous Giemsa-positive metachromatic intracytoplasmic granules leading to a diagnosis of subcutaneous mast cell tumor (MCT). Immunohistochemical analysis demonstrated positive staining for KIT with a diffuse cytoplasmic pattern, confirming the mastocytic origin. The tumor had histologic features suggestive of malignant behavior, which was confirmed by the metastatic mast cells in the regional lymph node adjacent to the tumor. MCT has not been reported previously in a chiropteran species, to our knowledge. Based on this case, MCTs in this species have malignant potential, and their diagnosis may require additional stains and immunohistochemistry (IHC). Our results support further investigation regarding the utility and prognostic value of KIT IHC in bats in this context.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251324717"},"PeriodicalIF":1.2,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143615799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of porcine circoviruses 2 and 3 in wild boar in Serbia.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-11 DOI: 10.1177/10406387251325534

Sofija Šolaja, Dimitrije Glišić, Vesna Milićević

{"title":"Prevalence of porcine circoviruses 2 and 3 in wild boar in Serbia.","authors":"Sofija Šolaja, Dimitrije Glišić, Vesna Milićević","doi":"10.1177/10406387251325534","DOIUrl":"10.1177/10406387251325534","url":null,"abstract":"Porcine circovirus 2 (PCV2) and porcine circovirus 3 (PCV3) are prevalent pathogens that are economically important to the global swine industry. We investigated the prevalence of PCV2 and PCV3 in eastern Serbia by analyzing 166 wild boar samples with a real-time PCR assay. The cumulative prevalence of PCV2 and PCV3 was 72.7%, with true prevalence estimates of 59.2% for PCV2, 49.0% for PCV3, and 31.0% for coinfection. We found higher prevalence of PCV2 and PCV3 in the Južnobanatski district compared to previous reports, likely due to differences in detection methods and the inclusion of liver samples. Backyard farms, with free-ranging animals and natural breeding, dominate eastern Serbia, facilitating frequent interactions between wild and domestic pigs. The overlap between backyard and commercial farming practices, along with high wild boar densities and cross-border movements, likely facilitate viral transmission. These conditions, coupled with inadequate biosecurity measures, such as swill feeding and improper disposal of offal, amplify the risk for viral dissemination, with significant implications for spillover into domestic pig populations. Enhanced biosecurity and monitoring strategies are crucial to mitigate the risk of pathogen transmission between wild and domestic pig populations.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251325534"},"PeriodicalIF":1.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904918/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pasteurella canis-associated bronchopneumonia in a 17-week-old Rottweiler and literature review.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-11 DOI: 10.1177/10406387251325161

Frank V Pellegrini, Lorelei L Clarke

引用次数: 0

Congenital neoplasms in cattle: A literature review and multi-institutional case series.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-11 DOI: 10.1177/10406387251324512

Emiliano Sosa, Federico Giannitti, Melissa Macías-Rioseco, Luis A Colque Caro, Caroline da Silva Silveira, Juan A García, María V Scioli, Eleonora Morrell, Dadin P Moore, Francesca Chianini, Germán J Cantón

引用次数: 0

Yersinia pseudotuberculosis and Y. enterocolitica abortions in sheep and goats in California: a series of cases diagnosed at CAHFS laboratories, 2002-2023.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-11 DOI: 10.1177/10406387251324883

Seung-Hee Cho, Aslı Mete, Isaiah Cueva, Melissa Macías-Rioseco, Heather Fritz, Nicolas Streitenberger, Omar Gonzales-Viera

{"title":"Yersinia pseudotuberculosis and Y. enterocolitica abortions in sheep and goats in California: a series of cases diagnosed at CAHFS laboratories, 2002-2023.","authors":"Seung-Hee Cho, Aslı Mete, Isaiah Cueva, Melissa Macías-Rioseco, Heather Fritz, Nicolas Streitenberger, Omar Gonzales-Viera","doi":"10.1177/10406387251324883","DOIUrl":"10.1177/10406387251324883","url":null,"abstract":"Abortion in small ruminants poses a significant economic threat and can have zoonotic risk. Although the association between yersiniosis and reproductive complications is known, systematic studies and case series on abortion in sheep and goats are scarce. Here we describe epidemiologic and pathologic findings in 34 cases of Yersinia pseudotuberculosis- and Y. enterocolitica-associated abortions in sheep and goats, contributing to the understanding of these zoonotic diseases in California. We conducted a 22-y retrospective study to examine microbiologic and pathologic findings in abortion submissions, as well as the geographic and seasonal distribution of the analyzed cases. Yersiniosis-induced abortion was diagnosed in 22 goats and 12 sheep, with all abortions occurring in the last third of gestation. Samples from lung, liver, placenta, and abomasal contents were submitted for aerobic culture; the highest recovery of Yersinia spp. was from abomasal contents. Microscopically, there was severe necrotizing and suppurative inflammation in the lung, liver, spleen, kidney, and, when present, the placenta, with intralesional bacterial colonies. All cases were received from northern and central California in the winter and spring. Our study provides epidemiologic and pathologic features of Yersinia spp.-induced abortions in small ruminants and provides critical data to pave the way for future research, surveillance, and preventive strategies.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251324883"},"PeriodicalIF":1.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bilateral intraocular choristoma in a 2-day-old foal.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-11 DOI: 10.1177/10406387251324997

Jasmine Morgan, Gillian Curtis Shaw, Jaime Weisman, Thomas Cecere, Francisco R Carvallo-Chaigneau

引用次数: 0

Fatal tracheal collapse and ossification in an osteopenic captive Indian blue peafowl in California.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-02 DOI: 10.1177/10406387251323870

Carlos Daniel Gornatti-Churria, Javier Asin, Aníbal G Armién, Veronica Nguyen, Simone T Stoute

引用次数: 0

A novel herpesvirus in blue penguins putatively associated with myocardial degeneration and necrosis. 一种新的疱疹病毒在蓝企鹅中被认为与心肌变性和坏死有关。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2024-12-26 DOI: 10.1177/10406387241309859

Tracie A Seimon, Batya R Nightingale, Martha A Delaney, Robert Moore, Charles E Alex, James F X Wellehan, Denise McAloose

{"title":"A novel herpesvirus in blue penguins putatively associated with myocardial degeneration and necrosis.","authors":"Tracie A Seimon, Batya R Nightingale, Martha A Delaney, Robert Moore, Charles E Alex, James F X Wellehan, Denise McAloose","doi":"10.1177/10406387241309859","DOIUrl":"10.1177/10406387241309859","url":null,"abstract":"We identified a novel herpesvirus in 2 deceased captive blue penguins (Eudyptula minor). Moderate-to-severe myocardiocyte atrophy and necrosis, and eosinophilic intranuclear inclusion bodies (INIBs), were seen in myocardiocytes in one bird; reticuloendothelial (RE) cell INIBs and multifocal RE cell necrosis were seen in both birds. The histologic findings were suggestive of viral infection. A herpesvirus PCR assay was positive in myocardial tissue from the bird with myocardial degeneration and in splenic tissue from both birds. Sequencing and phylogenetic analysis showed that the virus, accessioned as spheniscid alphaherpesvirus 2 (SpAHV2), groups within the Alphaherpesvirinae subfamily and forms a unique branch point in a subclade containing members of the Mardivirus, Simplexvirus, and Varicellovirus genera. Herpesvirus screening of tissues from 8 additional blue penguin postmortem examination cases (7 spleen, 1 liver) and combined conjunctival-choanal-cloacal swab samples from 13 live penguins revealed 5 additional dead and 7 live penguins that were positive for SpAHV2. The presence of SpAHV2 in healthy live animals and lack of significant herpesvirus-associated lesions as the cause of death in 6 of 7 SpAHV2-positive dead penguins suggests that this virus may be an endemic in blue penguins, and that recrudescence may cause disease and death.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"334-339"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and analysis of enzootic nasal tumor virus 2 in China. 中国流行性鼻肿瘤病毒 2 的检测与分析。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-06 DOI: 10.1177/10406387241310204

Pengfei Li, Xiaoan Cao, Jinyan Wu, Xiaobo Liu, Shouhui Mao, Ligang Yuan, Youjun Shang

{"title":"Detection and analysis of enzootic nasal tumor virus 2 in China.","authors":"Pengfei Li, Xiaoan Cao, Jinyan Wu, Xiaobo Liu, Shouhui Mao, Ligang Yuan, Youjun Shang","doi":"10.1177/10406387241310204","DOIUrl":"10.1177/10406387241310204","url":null,"abstract":"Enzootic nasal tumor virus 2 (ENTV2), the etiologic agent of enzootic nasal adenocarcinoma (ENA) in goats, is highly prevalent in China and causes significant economic losses to the goat industry. Here we describe the occurrence of ENA on a Dazu black goat farm in Chongqing City. At autopsy, nasal cavity masses were observed within the nose of an affected goat; histologically, the tumor was a nasal adenocarcinoma. The qPCR results demonstrated unequivocally that ENTV2 was the primary pathogen responsible for the tumor in this goat. We also collected nasal swab samples from all 180 goats on the farm; 9 goats tested positive for ENTV2. We generated the sequence of the full-length genome of ENTV2 (named ENTV2CQ, GenBank OR024676) with 7,469 nucleotides from nasal tumors from our case. ENTV2CQ shared the highest nucleotide identity with a previously sequenced isolate, ENTV2FJ (GenBank MK559457.1). ENTV2CQ and ENTV2FJ are located in the same major phylogenetic branch, mainly related to isolates from China from 2015 to 2022, and their phylogeny may be clustered geographically.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"244-251"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11701905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a qPCR assay to identify and differentiate insect-associated strains of the Serratia marcescens complex.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-27 DOI: 10.1177/10406387241313448

Nicholas P Doidge, Joanne L Allen, Rhys Bushell, Michael Lynch, Glenn F Browning, Marc S Marenda

{"title":"Development of a qPCR assay to identify and differentiate insect-associated strains of the Serratia marcescens complex.","authors":"Nicholas P Doidge, Joanne L Allen, Rhys Bushell, Michael Lynch, Glenn F Browning, Marc S Marenda","doi":"10.1177/10406387241313448","DOIUrl":"10.1177/10406387241313448","url":null,"abstract":"The Serratia marcescens complex contains important opportunistic pathogens of humans and vertebrate animals, as well as insects and other invertebrates. To date, the methods used for the identification of species within the genus Serratia, including PCR assays, have poor discriminatory power and may require further molecular typing or genomic sequence analysis to determine clinical relevance. We developed a duplex TaqMan probe-based quantitative real-time PCR (qPCR) assay targeting the chiP gene, which is involved in chitin degradation and transport, and the ureD gene, which is involved in urease production. This allowed us to simultaneously identify all members of the S. marcescens complex (chiP positive) and differentiate those most likely to act as insect pathogens (chiP and ureD positive). We applied our assay to identify potentially entomopathogenic members of the S. marcescens complex in the context of a conservation program for the critically endangered insect Dryococelus australis and found it to be a useful aid for rapid and accurate detection of infection with S. marcescens complex strains in insects and determination of their clinical relevance. By targeting 2 genes likely to be virulence factors, this assay may also be of use for research investigating the pathogenesis of entomopathogenic Serratia spp.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"234-243"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773504/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0