Journal of Veterinary Diagnostic Investigation最新文献_第2页

Human hepatoma HepG2 cells are susceptible to infection by domestic cat hepadnavirus. 人肝癌HepG2细胞易受家猫肝病毒感染。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-12 DOI: 10.1177/10406387251339765

Sabrina Wahyu Wardhani, Tanit Kasantikul, Chutchai Piewbang, Somporn Techangamsuwan

{"title":"Human hepatoma HepG2 cells are susceptible to infection by domestic cat hepadnavirus.","authors":"Sabrina Wahyu Wardhani, Tanit Kasantikul, Chutchai Piewbang, Somporn Techangamsuwan","doi":"10.1177/10406387251339765","DOIUrl":"10.1177/10406387251339765","url":null,"abstract":"Domestic cat hepadnavirus (DCHBV; Hepadnaviridae, Orthohepadnavirus felisdomestici) is a hepadnavirus identified in domestic cats that is associated with liver conditions that resemble hepatitis B virus (HBV) infections in humans. Despite its significance for feline health, research on DCHBV has been hindered due to the absence of suitable in vitro models, particularly the lack of feline hepatocyte cell lines. We found that cells of the human-derived hepatoma cell line, HepG2, were susceptible to DCHBV infection. DCHBV DNA and RNA were detected from the infected HepG2 cells at 48 h postinfection; DCHBV core protein and viral particles were detected within the infected cells. No significant enhancement of viral infection was observed by the addition of dimethyl sulfoxide or dexamethasone to the culture medium. However, higher levels of viral uptake were noted at 4 d postinfection when using a concentrated viral inoculum. Although HepG2 cells supported initial DCHBV infection, viral load declined over time during serial infection experiments. The DCHBV infection of HepG2 cells is limited and declines over successive passages, potentially due to insufficient cellular factors for sustained viral replication. Our findings establish a foundation for further investigations of DCHBV biology and highlight the necessity for identifying alternative cell lines to improve long-term infection models.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"609-619"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12069310/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pasteurella canis-associated bronchopneumonia in a 17-week-old Rottweiler and literature review. 17周龄罗威纳犬犬巴斯德氏菌相关性支气管肺炎及文献综述

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-11 DOI: 10.1177/10406387251325161

Frank V Pellegrini, Lorelei L Clarke

引用次数: 0

Congenital neoplasms in cattle: a literature review and multi-institutional case series. 牛先天性肿瘤：文献综述和多机构病例系列。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-11 DOI: 10.1177/10406387251324512

Emiliano Sosa, Federico Giannitti, Melissa Macías-Rioseco, Luis A Colque Caro, Caroline da Silva Silveira, Juan A García, María V Scioli, Eleonora Morrell, Dadin P Moore, Francesca Chianini, Germán J Cantón

引用次数: 0

T- and B-cell lymphomas in 2 captive green tree pythons. 2条圈养绿树蟒的T细胞和b细胞淋巴瘤。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-13 DOI: 10.1177/10406387251337369

Sara Pagliarani, Luke Haydock, Leonardo Susta, Pauline Delnatte, Cédric B Larouche, Mauricio Seguel

{"title":"T- and B-cell lymphomas in 2 captive green tree pythons.","authors":"Sara Pagliarani, Luke Haydock, Leonardo Susta, Pauline Delnatte, Cédric B Larouche, Mauricio Seguel","doi":"10.1177/10406387251337369","DOIUrl":"10.1177/10406387251337369","url":null,"abstract":"Two captive 19-y-old green tree pythons (Morelia viridis), a male and a female, were diagnosed with lymphoma. At autopsy, the female was in poor body condition and had an extensive subcutaneous soft tissue mass along the ribs. The mass was composed of numerous neoplastic round cells that were also present in the liver and spleen and within blood vessels. Neoplastic cells had marked, diffuse membranous-to-cytoplasmic immunolabeling for CD3, consistent with disseminated leukemic T-cell lymphoma. The male had a history of chronic spinal deformities and was in poor body condition. All examined organs contained variable numbers of neoplastic round cells with moderate-to-marked nuclear-to-cytoplasmic immunolabeling for PAX5, consistent with B-cell lymphoma. Here we describe 2 distinct immunophenotypes of lymphomas, including a B-cell neoplasm that has not been reported previously in snakes, to our knowledge. Our 2 cases highlight the potential value of CD3 and PAX5 immunohistochemical markers in ophidians and expand the spectrum of neoplastic diseases documented in reptiles. Understanding the clinical significance of lymphoma in snakes, including its potential impact on prognosis and challenges in management, is critical to refining therapeutic approaches in captive reptile care.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"647-651"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Renal oncocytoma in a chimpanzee. 黑猩猩肾脏嗜瘤细胞瘤。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-01 DOI: 10.1177/10406387251332203

Elliott S Chiu, William Vernau, Anibal G Armién, Verena K Affolter

引用次数: 0

Spontaneous branchioblastoma in a koi carp. 锦鲤自发性成鳃细胞瘤的研究。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-28 DOI: 10.1177/10406387251324885

Benjamin Balducchi, Eileen Henderson

引用次数: 0

A review of bovine anaplasmosis (Anaplasma marginale) with emphasis on epidemiology and diagnostic testing. 牛无形体病（边缘无形体）的综述，重点是流行病学和诊断测试。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-28 DOI: 10.1177/10406387251324180

Rosalie A Ierardi

{"title":"A review of bovine anaplasmosis (Anaplasma marginale) with emphasis on epidemiology and diagnostic testing.","authors":"Rosalie A Ierardi","doi":"10.1177/10406387251324180","DOIUrl":"10.1177/10406387251324180","url":null,"abstract":"Bovine anaplasmosis, caused by the rickettsia Anaplasma marginale, is an economically important tick-borne disease of cattle that is found worldwide. Its clinical effects of severe anemia, decreased growth, weight loss, and death negatively impact cattle welfare and create a significant economic burden for cattle producers. Despite availability of highly sensitive and specific assays for anti-A. marginale antibodies (competitive ELISA) and A. marginale genetic material (real-time PCR), the interpretation of test results for the diagnosis of clinical anaplasmosis in cattle remains challenging. Treatment and control usually consist of administration of oral and/or injectable tetracyclines; however, this approach is unlikely to be sustainable in the face of increasing scrutiny of antimicrobial usage in livestock. Statistically robust prospective studies are needed to characterize the prevalence, distribution, and transmission of bovine anaplasmosis under field conditions, as the epidemiology of this disease remains incompletely understood. Apart from minimizing exposure of naïve cattle to carriers (e.g., testing new introductions and interpreting in the context of herd-level seropositivity, changing needles between cattle), veterinarians and producers have few tools for prevention of bovine anaplasmosis based on data-driven risk assessment. A vaccine that is consistently safe and effective has proved elusive, but ongoing research into A. marginale vaccine candidates offers hope for a more effective means of protecting cattle from this costly disease.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"517-538"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11955989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of a commercial ELISA, an in-house indirect ELISA, and a dot-ELISA developed for the serodetection of Toxoplasma gondii antibodies in farm animals. 用于农场动物弓形虫抗体血清检测的商业ELISA、内部间接ELISA和点ELISA的比较。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-27 DOI: 10.1177/10406387251331637

Nagwa I Toaleb, Raafat M Shaapan, Dina Aboelsoued

{"title":"Comparison of a commercial ELISA, an in-house indirect ELISA, and a dot-ELISA developed for the serodetection of Toxoplasma gondii antibodies in farm animals.","authors":"Nagwa I Toaleb, Raafat M Shaapan, Dina Aboelsoued","doi":"10.1177/10406387251331637","DOIUrl":"10.1177/10406387251331637","url":null,"abstract":"Toxoplasma gondii is a widespread intracellular protozoan that can infect humans and animals. We isolated T. gondii strains from sheep, goats, cattle, buffaloes, and camels to develop and evaluate a modified in-house dot-ELISA for the detection of Toxoplasma antibodies in farm animals, and compared the results with a commercial ELISA (IDvet; gold standard). Animal tissue samples (n = 430) were examined microscopically, and infected tissues were bioassayed in mice as a viability test. Egyptian Toxoplasma strains were isolated from sheep, cattle, and camels and identified via PCR using the B1 gene (GenBank OR837022.1, OR837021.1, OR837020.1 from sheep, cattle, and camels, respectively). A T. gondii tachyzoite antigen from a sheep strain had the highest potential for the detection of specific T. gondii antibodies. We characterized this antigen using SDS-PAGE and separated it into 10 polypeptides of 96-12 kDa. Our modified in-house dot-ELISA detected T. gondii seropositivity in 172 of 430 (40%) farm animals with a sensitivity of 96.6% and specificity of 100%. The results of our dot-ELISA were confirmed in comparison with those of our indirect ELISA and the commercial ELISA. In a western blot, a predominant immunogenic reactive antigen band of 65 kDa was detected in T. gondii-positive sera of sheep, cattle, buffaloes, and camels; no cross-reaction occurred with antibodies to other parasitic infections or samples from healthy controls. Our modified in-house dot-ELISA is a rapid and simple test that showed promise for the detection of Toxoplasma antibodies in farm animals.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"597-608"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12037543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation of Clostridium botulinum type C from a wound in a pig. 猪伤口中C型肉毒杆菌的分离。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-10 DOI: 10.1177/10406387251330885

Benedetta Cordioli, Manuel Garbuio, Eliana Schiavon, Alessia Rizzardi, Angela Guolo, Serena Genovese, Ilenia Drigo, Luca Bano

引用次数: 0

Psittacine beak and feather disease in 2 free-living great green macaws: a case report and literature review. 2只自由生活大绿金刚鹦鹉鹦鹉喙羽病1例报告并文献复习。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-16 DOI: 10.1177/10406387251333410

Roberto W I Olivares, Laura G Bass, Andrés Sáenz-Bräutigam, Janet Sandí-Carmiol, Ana M Villada-Rosales, Gaby Dolz, Antony Solórzano-Morales, María J Zúniga-Moya, Roxana Granados-Solano, Brittany McHale, Diego S Zúñiga-Cortés, Francisco A Uzal

{"title":"Psittacine beak and feather disease in 2 free-living great green macaws: a case report and literature review.","authors":"Roberto W I Olivares, Laura G Bass, Andrés Sáenz-Bräutigam, Janet Sandí-Carmiol, Ana M Villada-Rosales, Gaby Dolz, Antony Solórzano-Morales, María J Zúniga-Moya, Roxana Granados-Solano, Brittany McHale, Diego S Zúñiga-Cortés, Francisco A Uzal","doi":"10.1177/10406387251333410","DOIUrl":"10.1177/10406387251333410","url":null,"abstract":"Psittacine beak and feather disease (PBFD) is caused by the psittacine beak and feather disease virus (PBFDV; Circoviridae, Circovirus parrot). This disease affects mainly young captive birds, inducing lesions mainly in the skin adnexa and lymphoid organs. Here we report PBFD in 2 free-living great green macaws (Ara ambiguus). The birds were depressed and had lost feathers and body condition. The diagnosis was confirmed by gross lesions and PCR testing in one bird, and by gross and microscopic lesions, PCR testing, viral sequencing, and in situ hybridization in the other bird. Gross lesions in both birds included mild beak discoloration and feather loss. Microscopic lesions in the bird whose tissues were examined histologically included bronchopneumonia and severe lymphoid depletion with intracytoplasmic and intranuclear botryoid inclusion bodies in the cloacal bursa. Sequences of the viral DNA obtained from paraffin-embedded cloacal bursa tissue had 100% nucleotide and 100% amino acid identity with several strains of PBFDV isolated from captive birds in multiple countries. To our knowledge, PBFD has not been reported previously in free-living great green macaws.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"666-673"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0