Journal of Veterinary Diagnostic Investigation最新文献_第3页

Yersinia pseudotuberculosis and Y. enterocolitica abortions in sheep and goats in California: a series of cases diagnosed at CAHFS laboratories, 2002-2023. 加利福尼亚绵羊和山羊的假结核耶尔森菌和小肠结肠炎耶尔森菌流产：2002-2023年CAHFS实验室诊断的一系列病例

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-11 DOI: 10.1177/10406387251324883

Seung-Hee Cho, Aslı Mete, Isaiah Cueva, Melissa Macías-Rioseco, Heather Fritz, Nicolas Streitenberger, Omar Gonzales-Viera

{"title":"Yersinia pseudotuberculosis and Y. enterocolitica abortions in sheep and goats in California: a series of cases diagnosed at CAHFS laboratories, 2002-2023.","authors":"Seung-Hee Cho, Aslı Mete, Isaiah Cueva, Melissa Macías-Rioseco, Heather Fritz, Nicolas Streitenberger, Omar Gonzales-Viera","doi":"10.1177/10406387251324883","DOIUrl":"10.1177/10406387251324883","url":null,"abstract":"Abortion in small ruminants poses a significant economic threat and can have zoonotic risk. Although the association between yersiniosis and reproductive complications is known, systematic studies and case series on abortion in sheep and goats are scarce. Here we describe epidemiologic and pathologic findings in 34 cases of Yersinia pseudotuberculosis- and Y. enterocolitica-associated abortions in sheep and goats, contributing to the understanding of these zoonotic diseases in California. We conducted a 22-y retrospective study to examine microbiologic and pathologic findings in abortion submissions, as well as the geographic and seasonal distribution of the analyzed cases. Yersiniosis-induced abortion was diagnosed in 22 goats and 12 sheep, with all abortions occurring in the last third of gestation. Samples from lung, liver, placenta, and abomasal contents were submitted for aerobic culture; the highest recovery of Yersinia spp. was from abomasal contents. Microscopically, there was severe necrotizing and suppurative inflammation in the lung, liver, spleen, kidney, and, when present, the placenta, with intralesional bacterial colonies. All cases were received from northern and central California in the winter and spring. Our study provides epidemiologic and pathologic features of Yersinia spp.-induced abortions in small ruminants and provides critical data to pave the way for future research, surveillance, and preventive strategies.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"545-551"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143605373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro evaluation of porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 sequences in samples containing PRRSV modified-live vaccine and wild-type strains. 猪繁殖与呼吸综合征病毒（PRRSV）修饰活疫苗和野生型毒株ORF5序列的体外评价

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-15 DOI: 10.1177/10406387251340342

Joaquín Alvarez-Norambuena, Albert Rovira, Cesar A Corzo, Mariana Kikuti

{"title":"In vitro evaluation of porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 sequences in samples containing PRRSV modified-live vaccine and wild-type strains.","authors":"Joaquín Alvarez-Norambuena, Albert Rovira, Cesar A Corzo, Mariana Kikuti","doi":"10.1177/10406387251340342","DOIUrl":"10.1177/10406387251340342","url":null,"abstract":"Porcine reproductive and respiratory syndrome (PRRS) causes significant economic losses and is a major challenge to the swine industry. The PRRS virus (PRRSV) has high rates of mutation and evolution. We examined the influence on ORF5 Sanger sequencing outcomes of various concentrations of a wild-type (WT) PRRSV (lineage 1A RFLP 1-7-4) and a modified-live vaccine (MLV) virus (lineage 5 RFLP 2-5-2) in the same sample. Vaccine-like sequences were detected more frequently than the WT virus when the MLV virus was present at equal or higher concentrations than the WT virus. This result suggests that ORF5 Sanger sequencing may preferentially detect the dominant virus in samples containing more than one virus, potentially masking WT viral infections in vaccinated herds. Although our findings highlight a limitation in identifying co-circulation of strains, Sanger sequencing is still widely used as an accessible tool to characterize PRRSVs. Advanced sequencing techniques, such as NGS or CLAMP-based approaches, would complement Sanger sequencing results and allow improved detection of co-circulating variants by minimizing consensus sequence bias and selectively blocking vaccine-like sequences.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"716-721"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reference intervals for trace minerals and heavy metals in livers of cattle in wilderness areas in the Netherlands. 荷兰荒野地区牛肝脏中微量矿物质和重金属的参考区间。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-24 DOI: 10.1177/10406387251325864

Deon van der Merwe, Yannique Jacobs, Saskia van der Drift, Ant Koopmans

{"title":"Reference intervals for trace minerals and heavy metals in livers of cattle in wilderness areas in the Netherlands.","authors":"Deon van der Merwe, Yannique Jacobs, Saskia van der Drift, Ant Koopmans","doi":"10.1177/10406387251325864","DOIUrl":"10.1177/10406387251325864","url":null,"abstract":"We examined the liver mineral concentrations of semi-wild cattle breeds (Highland, Galloway, Rode Geus) grazing in natural or near-natural flood-prone areas in the Netherlands. These breeds are employed as part of trophic rewilding initiatives aimed at maintaining biodiverse ecosystems through natural grazing patterns. Our primary goal was to derive RIs for liver mineral concentrations for these cattle populations. We collected and analyzed liver samples from 96 cattle from 14 sites in the Netherlands and used inductively coupled plasma mass spectrometry to measure concentrations of trace elements and heavy metals, including arsenic, cadmium, chromium, cobalt, copper, iron, lead, manganese, molybdenum, nickel, selenium, vanadium, and zinc. The statistical analysis included Pearson product moment correlations and k-means clustering to evaluate patterns within the data. The highest significant correlations were between liver cadmium concentrations and animal age, and between liver copper and selenium concentrations. Breed-related differences were found for liver cadmium and molybdenum concentrations, but we could not isolate breed-differences in forage selection from environmental variables such as soil composition. Liver copper levels in many animals were below thresholds typically used to indicate copper deficiency in farmed cattle.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"552-558"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adenofibroma of the uterine tube in a pig. 猪输卵管腺纤维瘤。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-28 DOI: 10.1177/10406387251324670

Diana A Bochynska, Corina G Toma, Sara Salgado, Ann P Donachey, Dana M Sheets-Nichols, Robert A Foster

引用次数: 0

Acute fatal Leptospira kirschneri infection in suckling lambs and calves in Uruguay. 乌拉圭哺乳羔羊和小牛急性致死性克氏钩端螺旋体感染。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-12 DOI: 10.1177/10406387251341234

Mizael Machado, Camila Ciuffo, Federico Giannitti, Caroline da Silva Silveira, Yisell Perdomo, Cintia R R Queiroz-Machado, Rafael Carriquiry, Leticia Zarantonelli, Alejo Menchaca

{"title":"Acute fatal Leptospira kirschneri infection in suckling lambs and calves in Uruguay.","authors":"Mizael Machado, Camila Ciuffo, Federico Giannitti, Caroline da Silva Silveira, Yisell Perdomo, Cintia R R Queiroz-Machado, Rafael Carriquiry, Leticia Zarantonelli, Alejo Menchaca","doi":"10.1177/10406387251341234","DOIUrl":"10.1177/10406387251341234","url":null,"abstract":"Leptospira kirschneri is an emerging human and animal pathogen. Here we describe 2 unrelated outbreaks of acute fatal leptospirosis caused by L. kirschneri in suckling lambs and calves diagnosed following a flooding event in the spring of 2023 in northern Uruguay. In outbreak 1, the sheep flock was raised in a low-lying area previously used for rice cultivation; 20 lambs died after developing apathy, jaundice, and hemoglobinuria. In outbreak 2, 2 calves were found dead. At autopsy, 4 lambs and 2 calves had marked jaundice, hemoglobinuria, dark-red kidneys, and hepatomegaly. Microscopically, we found marked hemoglobinuric nephrosis, lymphohistiocytic tubulointerstitial nephritis, hepatocellular dissociation, and random hepatocellular necrosis with periportal lymphohistiocytic hepatitis and canalicular cholestasis. Positive immunostaining for Leptospira sp. was found in the liver (lamb C), both the liver and kidney (calf B), and the liver (calf A). Leptospira kirschneri was PCR-confirmed using kidney and liver samples from the autopsied animals. Acute leptospirosis caused by L. kirschneri has not been reported previously in sheep and cattle, to our knowledge. Our findings highlight that L. kirschneri infection can cause acute leptospirosis in unvaccinated lambs and calves.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"679-683"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of fibrinogen Aα-chain as an amyloid precursor protein in a case of canine coronary artery amyloidosis. 纤维蛋白原a α-链在犬冠状动脉淀粉样变性中作为淀粉样前体蛋白的鉴定。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-23 DOI: 10.1177/10406387251331933

Susumu Iwaide, Garrett L Wachoski-Dark, Tomoaki Murakami, Niki Sedghi Masoud, Yoshiyuki Itoh, Miki Hisada, Kumiko Kimura, Tomoyuki Shibahara, Makoto Haritani, Jeff L Caswell

{"title":"Identification of fibrinogen Aα-chain as an amyloid precursor protein in a case of canine coronary artery amyloidosis.","authors":"Susumu Iwaide, Garrett L Wachoski-Dark, Tomoaki Murakami, Niki Sedghi Masoud, Yoshiyuki Itoh, Miki Hisada, Kumiko Kimura, Tomoyuki Shibahara, Makoto Haritani, Jeff L Caswell","doi":"10.1177/10406387251331933","DOIUrl":"10.1177/10406387251331933","url":null,"abstract":"Spontaneous amyloid deposition occurs in the coronary arteries in older dogs, but the pathogenesis, including the precursor protein, is not known. We conducted proteome analysis using liquid chromatography-tandem mass spectrometry to identify the amyloid precursor protein in a 13-y-old male mixed-breed dog with amyloid deposition in intramural coronary arteries, and identified fibrinogen Aα-chain. This form of amyloidosis is characterized by the deposition of amyloid derived from fibrinogen Aα-chains, which has been reported previously only in humans and Japanese squirrels. Within amyloid deposits in the intramural coronary arteries of our canine case, the αC connector region was identified in abundance, whereas in humans and Japanese squirrels, the amyloid arises from the αC domain. Thus, even if amyloid is formed by the same protein, the amyloid-forming peptide and the anatomic site of deposition in our case differed from that in humans and Japanese squirrels.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"689-693"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12018354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bilateral intraocular choristoma in a 2-day-old foal. 2日龄马驹双侧眼内脉络膜瘤。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-11 DOI: 10.1177/10406387251324997

Jasmine Morgan, Gillian Curtis Shaw, Jaime Weisman, Thomas Cecere, Francisco R Carvallo-Chaigneau

引用次数: 0

Canine polyostotic B-cell lymphoma: a case with clinical, immunohistochemical, and flow cytometric characterization, and review of the literature. 犬多骨增生b细胞淋巴瘤：一例临床、免疫组织化学和流式细胞术特征，并复习文献。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-03-21 DOI: 10.1177/10406387251329020

Matthew Kornya, Connor Bryant, Brandon Lillie, Sebastien Sanz, Kristiina Ruotsalo, Dorothee Bienzle

{"title":"Canine polyostotic B-cell lymphoma: a case with clinical, immunohistochemical, and flow cytometric characterization, and review of the literature.","authors":"Matthew Kornya, Connor Bryant, Brandon Lillie, Sebastien Sanz, Kristiina Ruotsalo, Dorothee Bienzle","doi":"10.1177/10406387251329020","DOIUrl":"10.1177/10406387251329020","url":null,"abstract":"An 8-mo-old Mastiff-cross dog with bone pain and lytic-proliferative lesions in the radius, ulna, femur, vertebral spinous processes, and ribs, was diagnosed with lymphoma. The dog also had anemia and thrombocytopenia, and atypical circulating lymphocytes were identified as B cells by flow cytometry. Due to the multicentric, rapidly progressive disease, the dog was euthanized. Postmortem examination confirmed extensive bone replacement by lymphoma, and infiltration of lymph nodes, spleen, and liver. Histomorphology and immunohistochemistry showed a diffuse large B-cell lymphoma that was immunopositive for PAX5 and CD20, and immunonegative for CD3. Lymphoma of bone is rare in dogs and humans, and is most frequently reported in pediatric individuals. Including our case, 7 of 14 reported cases occurred in dogs <2-y-old, and all but 1 had polyostotic disease. Long bones, ribs, and vertebrae were affected most often, and the distal metaphyseal region was targeted in long bones. Visceral and nodal tissue infiltration was common, and all tumors had a diffuse architecture. Most dogs with polyostotic lymphoma were euthanized at the time of diagnosis, and survival was <6 wk in dogs that were treated with chemotherapy or surgery.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"697-704"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11948266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiple spinal nerve sheath tumors in a dog resembling schwannomatosis in humans. 狗的多发性脊髓神经鞘肿瘤类似于人类的神经鞘瘤病。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-07 DOI: 10.1177/10406387251329049

Jun Lai Tse, Agustín Rebollada-Merino, Martí Pumarola, Ana Liza Ortiz, Cristina Toni

引用次数: 0

Concurrent pancreatic exocrine hyperplasia and adenomas in a Eurasian otter with pleuropneumonia and urolithiasis. 并发胰腺外分泌增生和腺瘤的欧亚水獭胸膜肺炎和尿石症。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-13 DOI: 10.1177/10406387251338327

Jeong-Eun Yeom, Min-Gyeong Seo, Kook-Young Han, Sang-Hyun Kim, Eun-Joo Lee, Kyu-Shik Jeong, Il-Hwa Hong

引用次数: 0