Journal of Veterinary Diagnostic Investigation最新文献_第3页

Comprehensive precision analysis of the MIZAR 2.0 hemorheometer in EDTA-anticoagulated canine whole blood samples. MIZAR 2.0血压计在edta抗凝犬全血样本中的综合精度分析。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-11-01 Epub Date: 2026-07-29 DOI: 10.1177/10406387261467756

Francisco O Conrado, Cynthia Leveille-Webster, Noa Berlin, Kady Marino, Jérémie Korchia

{"title":"Comprehensive precision analysis of the MIZAR 2.0 hemorheometer in EDTA-anticoagulated canine whole blood samples.","authors":"Francisco O Conrado, Cynthia Leveille-Webster, Noa Berlin, Kady Marino, Jérémie Korchia","doi":"10.1177/10406387261467756","DOIUrl":"10.1177/10406387261467756","url":null,"abstract":"Hemorheology, the study of blood flow properties, utilizes the deformability, elasticity, and aggregability of RBCs to assess health and disease. Although promising in human medicine for diagnosing and monitoring various conditions, its clinical utility in dogs remains unexplored. Given that assay validation is paramount for establishing RIs and interpreting hemorheologic data, we characterized the precision of 13 hemorheologic RBC measurands in dogs using a novel hemorheometer (MIZAR; Alcor Scientific). We investigated the influence of precision type (within- and between-run), temperature (room temperature and 4°C), number of repeats (n 8-22), and wavelength of reading (620 and 800 nm) on measurement CVs. We found 3 distinct measurand categories: consistently low CVs, exemplified by optical transmission flow (OTF); consistently high CVs, exemplified by distribution index (DI); and condition-dependent CVs, exemplified by aggregation index (AI). For measurands with consistently high CVs, n had a greater impact on imprecision than any other factor. All other measurands had expected patterns of imprecision, with within-run measurements having the lowest CVs. Interestingly, measurand values did not significantly impact CVs in healthy dogs. For most measurands, CVs varied among samples handled similarly, which could be related to inter-individual variation; this observation may not necessarily affect the clinical accuracy of the hemorheometer in assessing clinically relevant changes in hemorheologic measurands during disease states.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1151-1161"},"PeriodicalIF":1.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13423933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Old nodes, new tricks: optimized methods for chronic wasting disease prion detection in preserved retropharyngeal lymph nodes. 旧节点，新技巧：保存咽后淋巴结慢性消耗性疾病朊病毒检测的优化方法。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-11-01 Epub Date: 2026-05-21 DOI: 10.1177/10406387261445902

Avery Munster, Jennifer Høy-Petersen, Madison A Davis, Sarah A Tomke, Kevin D Niedringhaus, Roderick B Gagne, Michelle Gibison

{"title":"Old nodes, new tricks: optimized methods for chronic wasting disease prion detection in preserved retropharyngeal lymph nodes.","authors":"Avery Munster, Jennifer Høy-Petersen, Madison A Davis, Sarah A Tomke, Kevin D Niedringhaus, Roderick B Gagne, Michelle Gibison","doi":"10.1177/10406387261445902","DOIUrl":"10.1177/10406387261445902","url":null,"abstract":"Chronic wasting disease (CWD) is a highly transmissible, global prion disease of captive and wild cervids. The approved tests for the detection of the infectious CWD prion (PrPCWD) are ELISA and immunohistochemistry (IHC), but both have limitations and can yield discrepant results. ELISA requires a fresh or frozen medial retropharyngeal lymph node (RPLN); IHC requires the RPLN to be formalin-fixed and paraffin-embedded, thus requiring 2 different storage protocols. Real-time quaking-induced conversion (rtQuIC) is a sensitive amplification technique used commonly in research to detect prions. Here, we optimized a rtQuIC assay for CWD prion detection in formalin-fixed (FF) and formalin-fixed paraffin-embedded tissues (FFPE) from white-tailed deer in Pennsylvania with various prion protein (PRNP) genotypes. Our proposed protocol differs from other publications because it does not require hazardous solvents such as xylene. We found that FF RPLN samples can be tested by rtQuIC with up to 96% sensitivity and 100% specificity, and FFPE RPLN samples can be tested by rtQuIC with up to 98% sensitivity and 99% specificity for PrPCWD detection. Our results are comparable to testing fresh or frozen tissue, and we did not find an effect of prion protein genotype on assay performance. This application of rtQuIC has the potential to improve surveillance and disease management in regions with limited access to cold storage and allow testing of RPLNs with discrepant PrPCWD results when tissue of sufficient quality is unavailable.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1212-1221"},"PeriodicalIF":1.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13194330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147982320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of an in situ hybridization test for the diagnosis of paratuberculosis (Mycobacterium avium subsp. paratuberculosis infection) in goats. 原位杂交试验对副结核（鸟分枝杆菌亚种）诊断的评价。山羊的副结核感染。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-11-01 Epub Date: 2026-05-14 DOI: 10.1177/10406387261447257

Agustín Rebollada-Merino, Samantha L Shallop, Mia A Durham, Elena Alina Demeter

{"title":"Assessment of an in situ hybridization test for the diagnosis of paratuberculosis (Mycobacterium avium subsp. paratuberculosis infection) in goats.","authors":"Agustín Rebollada-Merino, Samantha L Shallop, Mia A Durham, Elena Alina Demeter","doi":"10.1177/10406387261447257","DOIUrl":"10.1177/10406387261447257","url":null,"abstract":"Paratuberculosis (PTB) is a chronic infection caused by Mycobacterium avium subsp. paratuberculosis (MAP). The gold standard test for detection of MAP is culture, which requires 8-16 wk. We reviewed archived intestinal cases of goat PTB and performed RNA scope-based chromogenic pan-mycobacterial in situ hybridization (ISH) on formalin-fixed paraffin-embedded (FFPE) tissue in 20 cases and 5 negative controls. Cases included 11 multibacillary and 9 paucibacillary infections, 2 of which had postmortem autolysis. At the time of diagnosis, we compared the results with ancillary testing (MAP culture and/or PCR testing of fresh tissues), Ziehl-Neelsen (ZN) staining, and pan-Mycobacterium PCR testing of FFPE tissues. MAP had been confirmed in 9 of 10 cultured cases. MAP had been detected by PCR in fresh tissue in 7 of 7 cases. We detected Mycobacterium spp. by PCR in FFPE tissue in 18 of 20 cases; the 2 negative cases had been confirmed at the time of diagnosis. We detected hybridization signal in 19 of 20 cases, including 8 of 9 paucibacillary cases, in 6 cases of which we did not detect bacilli by ZN; the case lacking hybridization signal had severe autolysis. We did not see hybridization signal in the negative controls. We found that ISH and PCR tests of FFPE tissues are more sensitive than ZN, can reduce turnaround time, and contribute to the diagnosis of PTB, especially when fresh tissue is unavailable for culture and PCR. Incorporation of ISH into an integrated diagnostic framework could aid in the confirmation of MAP infection.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1232-1237"},"PeriodicalIF":1.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13179308/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Noninflammatory hypotrichosis in white-tailed deer and raccoons in the eastern United States. 美国东部白尾鹿和浣熊的非炎症性毛少症。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-11-01 Epub Date: 2026-06-01 DOI: 10.1177/10406387261450605

C Robert Stilz, Taylor C Chan, Alainah Bhutta, Kevin D Niedringhaus, Caitlin E Burrell, Victoria A Andreasen, Lauren Maxwell, Elizabeth Signore, Adrianna Mowrer, Mark G Ruder, Nicole M Nemeth

{"title":"Noninflammatory hypotrichosis in white-tailed deer and raccoons in the eastern United States.","authors":"C Robert Stilz, Taylor C Chan, Alainah Bhutta, Kevin D Niedringhaus, Caitlin E Burrell, Victoria A Andreasen, Lauren Maxwell, Elizabeth Signore, Adrianna Mowrer, Mark G Ruder, Nicole M Nemeth","doi":"10.1177/10406387261450605","DOIUrl":"10.1177/10406387261450605","url":null,"abstract":"Noninflammatory hypotrichosis includes a spectrum of disorders with 2 phenotypes: follicular or ectodermal dysplasia. Here, we describe 6 follicular dysplasia cases in 2 wild adult male raccoons (Procyon lotor) and 4 wild yearling or adult white-tailed deer (Odocoileus virginianus), and 1 case of ectodermal dysplasia in a captive-reared stillborn male white-tailed deer, all from the eastern United States. All cases had regional or body-wide hairlessness. Skin hyperpigmentation was observed in those with follicular dysplasia. The stillborn fawn also had multiple developmental defects, including craniofacial dysplasia and unilateral anophthalmia. Histologic features of affected skin were heterogeneous. Follicles were typically normal in number and distribution, but their stage of development varied (primitive, anagen, telogen, kenogen, or a combination thereof). Hair shafts were either formed, poorly differentiated, or absent. Sebaceous glands were variably hypoplastic, normal, or hyperplastic. We did not definitively determine an underlying etiology; however, a genetic cause is suspected.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1255-1261"},"PeriodicalIF":1.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13226295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148138457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systemic salmonellosis in 3 pine siskins from Louisiana during the 2020-2021 songbird mortality event. 在2020-2021年鸣禽死亡事件期间，路易斯安那州3个松雀体内的系统性沙门氏菌病。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-11-01 Epub Date: 2026-08-12 DOI: 10.1177/10406387261470796

Hiba Ali, Emi Sasaki, Mark A Mitchell, Maria S Mitchell, Kristin Vyhnal, Daniel Paulsen, Mariano Carossino, Jeongha Lee

{"title":"Systemic salmonellosis in 3 pine siskins from Louisiana during the 2020-2021 songbird mortality event.","authors":"Hiba Ali, Emi Sasaki, Mark A Mitchell, Maria S Mitchell, Kristin Vyhnal, Daniel Paulsen, Mariano Carossino, Jeongha Lee","doi":"10.1177/10406387261470796","DOIUrl":"10.1177/10406387261470796","url":null,"abstract":"During the 2020-2021 irruption year, pine siskins (Spinus pinus) experienced widespread mortality across North America, attributed to Salmonella enterica subsp. enterica serovar Typhimurium. In March 2021, 3 pine siskins were admitted to the Louisiana State University Wildlife Hospital (Baton Rouge, LA, USA) because of lethargy, respiratory distress, diarrhea, and inability to fly. All birds died on or shortly after presentation. Postmortem examination revealed necrotizing and granulomatous esophagitis (3), hepatitis (3), splenitis (2), enteritis (1), cloacitis (1), and stomatitis/rhinitis (1) with intralesional gram-negative bacilli. Immunohistochemistry revealed abundant extracellular and intracellular Salmonella sp. lipopolysaccharide antigen in affected organs. Salmonella Typhimurium was isolated from livers (3), cloacal swabs (2), and a spleen (1). One case had concurrent trichomonosis with erosive pharyngitis and necrotizing esophagitis. These cases are a subset of the larger 2020-2021 salmonellosis outbreak in passerines and are among the few reported from Louisiana.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1330-1335"},"PeriodicalIF":1.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13473217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148713207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycobacterium shimoidei causing granulomatous inflammation in a captive bird species, the white-cheeked turaco. 引起圈养鸟类肉芽肿性炎症的shimoide分枝杆菌，白颊turaco。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-11-01 Epub Date: 2026-07-30 DOI: 10.1177/10406387261471130

Sara Pagliarani, Liz Orkhulik, Kate Hartzler, Chiara Palmieri, Olga Andrievskaia, Mirjana Savic, Mauricio Seguel

{"title":"Mycobacterium shimoidei causing granulomatous inflammation in a captive bird species, the white-cheeked turaco.","authors":"Sara Pagliarani, Liz Orkhulik, Kate Hartzler, Chiara Palmieri, Olga Andrievskaia, Mirjana Savic, Mauricio Seguel","doi":"10.1177/10406387261471130","DOIUrl":"10.1177/10406387261471130","url":null,"abstract":"Mycobacteriosis in avian species is generally caused by Mycobacterium avium subsp. avium and M. genavense, although sporadic reports also exist of other nontuberculous mycobacterium species in birds. Here, we describe disseminated mycobacteriosis caused by M. shimoidei (a rare and infrequently reported pathogen in humans and animals) in a bird species. A captive, adult white-cheeked turaco (Tauraco leucotis) was presented because of persistent dyspnea unresponsive to treatment. The animal was treated unsuccessfully with antimicrobials and later euthanized on welfare grounds. At autopsy, granulomas were identified in the lungs and spleen. Histologically, the coalescing granulomas with central necrosis contained numerous chains of slender, slightly curved, 2-µm acid-fast bacilli, suggesting mycobacterial infection. Bacterial isolation confirmed Mycobacterium spp., which was identified as M. shimoidei by partial sequencing of the 16S rRNA and rpoB genes. Our report expands the known host range of M. shimoidei and highlights the importance of molecular testing methods in identifying atypical nontuberculous mycobacteria (NTM) in zoologic collections. Our case also underscores the need for enhanced epidemiologic surveillance of NTM infections in avian species, given their potential implications for both animal and public health.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1262-1266"},"PeriodicalIF":1.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13423932/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exserohilum rostratum granulomatous rhinitis in a Holstein cow and review of the literature of diseases caused in domestic ruminants by this dematiaceous fungus and its historical synonyms. 荷斯坦牛棘芽孢杆菌肉芽肿性鼻炎及由该真菌及其历史同义词引起的家畜反刍动物疾病的文献综述。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-11-01 Epub Date: 2026-09-22 DOI: 10.1177/10406387261485968

Miguel Carrillo Parraguez, Carolina Matto, María de Lourdes Adrien, Serafín Ceriani, Yisell Perdomo, Tiffany L Rivers, Lalitha Gade, Jana M Ritter, Federico Giannitti

{"title":"Exserohilum rostratum granulomatous rhinitis in a Holstein cow and review of the literature of diseases caused in domestic ruminants by this dematiaceous fungus and its historical synonyms.","authors":"Miguel Carrillo Parraguez, Carolina Matto, María de Lourdes Adrien, Serafín Ceriani, Yisell Perdomo, Tiffany L Rivers, Lalitha Gade, Jana M Ritter, Federico Giannitti","doi":"10.1177/10406387261485968","DOIUrl":"10.1177/10406387261485968","url":null,"abstract":"Here, we describe a case of Exserohilum rostratum rhinitis in a cow and review the literature on diseases caused by Exserohilum spp. and dematiaceous fungi historically classified morphologically as Helminthosporium, Drechslera, or Bipolaris in domestic ruminants. In March 2024, an adult Holstein cow from a dairy herd in Uruguay developed bilateral nodules in the nasal mucosa, resulting in dyspnea, snoring, nasal discharge, and weight loss. Histologically, severe chronic eosinophilic granulomatous rhinitis was found, with giant cells and intra- and extracellular fungi. Exserohilum rostratum was identified by PCR and sequencing of the extended 28S rDNA region. Our literature search retrieved ~40 bovine cases in the United States, Australia, South Africa, Namibia, and Uruguay, 1 caprine case in Brazil, and no ovine cases. Clinical signs included chronic dyspnea, stertor, mucopurulent nasal discharge, epistaxis, and weight loss, generally affecting adult beef cattle. The lesions usually affected the nasal mucosa and submucosa, rarely involved the skin or regional lymph nodes, and occasionally were restricted to the skin. Bilateral, raised, 0.1-5-cm granulomatous nasal nodules resulted in restriction of airflow. The hallmark microscopic lesion is chronic eosinophilic granulomatous inflammation with giant cells and intralesional fungal structures, including ~5-30-µm, round-to-ovoid chlamydospores and short ~2-6-µm-wide septate hyphae, which may have brown-pigmented walls. Definitive fungal identification requires molecular testing, which is rarely pursued.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1121-1130"},"PeriodicalIF":1.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13597963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149017214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracerebral papillary ependymoma in a cow. 牛脑内乳头状室管膜瘤。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-11-01 Epub Date: 2026-05-14 DOI: 10.1177/10406387261444330

Stefano Bagatella, Ronja Kauer, Margherita Polidori, Anna Oevermann

{"title":"Intracerebral papillary ependymoma in a cow.","authors":"Stefano Bagatella, Ronja Kauer, Margherita Polidori, Anna Oevermann","doi":"10.1177/10406387261444330","DOIUrl":"10.1177/10406387261444330","url":null,"abstract":"Intracranial neoplasms are rare in cattle. Bovine ependymomas have been reported only sporadically, with limited morphologic descriptions and, to our knowledge, without immunohistochemical characterization. Here, we describe the gross, histologic, and immunophenotypic features of a papillary ependymoma in the brain of a 7.5-y-old cow that was a bovine spongiform encephalopathy suspect. Grossly, a large, intraparenchymal, well-demarcated mass occupied the left cerebral hemisphere, extending from the frontal to the occipital lobe and communicating with the left lateral ventricle. Histologically, the neoplasm was composed of elongated-to-columnar cells arranged predominantly in perivascular pseudorosettes with prominent papillary architecture. Immunohistochemically, >95% of neoplastic cells had strong cytoplasmic glial fibrillary acidic protein immunolabeling, which was accentuated in perivascular processes; ~20% of neoplastic cells were also immunolabeled for vimentin. Neoplastic cells lacked immunolabeling for pan-cytokeratin, S100, oligodendrocyte transcription factor 2, NeuN, synaptophysin, and E-cadherin. The combined morphologic and immunophenotypic findings supported a diagnosis of papillary ependymoma and excluded morphologic mimics.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1375-1379"},"PeriodicalIF":1.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13179287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147942221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clostridium perfringens gas gangrene in a canine splenic liposarcoma. 犬脾脂肪肉瘤的产气荚膜梭菌气性坏疽。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-11-01 Epub Date: 2026-07-29 DOI: 10.1177/10406387261465363

Michael R Hardcastle, Yasmine Messiaen, Francisco A Uzal

{"title":"Clostridium perfringens gas gangrene in a canine splenic liposarcoma.","authors":"Michael R Hardcastle, Yasmine Messiaen, Francisco A Uzal","doi":"10.1177/10406387261465363","DOIUrl":"10.1177/10406387261465363","url":null,"abstract":"Histotoxic clostridial infections occur when clostridial spores germinate in anaerobic tissue environments and release a variety of extracellular toxins, causing necrosis, secondary inflammation, and often death. These infections are seen in humans and animals, especially ruminants and horses, but are rare in dogs. An 11-y-old, intact male Border Collie-cross dog was inappetent and lethargic after abdominal blunt-force trauma. Clinical examination and imaging revealed a splenic mass containing gas and an abdominal effusion, identified cytologically as a neutrophilic exudate. At splenectomy, an area on the anti-hilar surface of the mass was abscessed, with inspissated material on the mass surface and in the omentum. Histopathology identified a splenic liposarcoma with areas of necrosis, suppuration, emphysema, and colonization by large gram-positive bacilli. These were identified as Clostridium perfringens by immunohistochemistry. The bacteria are presumed to have translocated from the bowel and germinated in a region of tumor necrosis secondary to traumatic ischemia. To our knowledge, gas gangrene has not been reported previously within a visceral tumor in a dog.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1357-1360"},"PeriodicalIF":1.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13423934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148620702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Escherichia marmotae, a novel pathogen causing urinary tract infection in a dog. 一种引起犬尿路感染的新型病原体——狨猴埃希氏菌的鉴定。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-11-01 Epub Date: 2026-07-08 DOI: 10.1177/10406387261461260

Wendy Cuevas-Espelid, Sonsiray Álvarez-Narváez, Susan Sánchez

{"title":"Characterization of Escherichia marmotae, a novel pathogen causing urinary tract infection in a dog.","authors":"Wendy Cuevas-Espelid, Sonsiray Álvarez-Narváez, Susan Sánchez","doi":"10.1177/10406387261461260","DOIUrl":"10.1177/10406387261461260","url":null,"abstract":"Escherichia is one of the most studied bacterial genera and includes several clinically significant pathogenic species. Unique strategies have evolved in members of this genus to allow their survival within different niches. Although initially the Escherichia cryptic clades (CCs) I-VIII were identified as environmental bacteria, they have been found as commensals and opportunistic pathogens in animals and humans. Notably, members of the Escherichia CCs are commonly mistaken for E. coli when using standard microbiologic techniques for species identification. We used whole-genome sequencing to correctly identify E. marmotae-a member of the Escherichia CCs-that was initially misidentified as E. coli as the causative agent of a urinary tract infection (UTI) in a female dog. The isolate had phenotypic and genotypic resistance to cefalexin-an antibiotic used commonly for the treatment of uncomplicated UTIs-and carried a set of virulence factors also present in uropathogenic E. coli, which would explain the capacity of the organism to colonize the urinary tract. As a relatively new species that is phenotypically very similar to E. coli, it is quite possible that E. marmotae infections may be underreported or misidentified. We hope that our work alerts veterinarians to consider E. marmotae as a possible cause of UTIs in dogs, especially because this could affect treatment decisions. Additionally, our findings broaden the current understanding of the E. marmotae ecology and host range.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1202-1211"},"PeriodicalIF":1.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13346104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148405035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0