Journal of Veterinary Diagnostic Investigation最新文献_第3页

Whole-genome sequencing of Salmonella serovars isolated from diarrheic and non-diarrheic foals.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-02-10 DOI: 10.1177/10406387251316314

Roberta M Basso, Fabrício M Cerri, Fábio S Possebon, Pollyana R C Braga, Monique R T Casas, José P de Oliveira-Filho, João P de Araújo Júnior, Márcio G Ribeiro, Luis G Arroyo, Alexandre S Borges

{"title":"Whole-genome sequencing of Salmonella serovars isolated from diarrheic and non-diarrheic foals.","authors":"Roberta M Basso, Fabrício M Cerri, Fábio S Possebon, Pollyana R C Braga, Monique R T Casas, José P de Oliveira-Filho, João P de Araújo Júnior, Márcio G Ribeiro, Luis G Arroyo, Alexandre S Borges","doi":"10.1177/10406387251316314","DOIUrl":"10.1177/10406387251316314","url":null,"abstract":"Salmonella spp. are important pathogens of foals, causing clinical enterocolitis and sepsis. We characterized the resistance, virulence, and stress response genes in Salmonella isolates from foals with or without diarrhea. Salmonella isolates (n = 23) recovered from fecal samples of 16 diarrheic and 7 non-diarrheic <1-y-old foals were subjected to whole-genome sequencing. The most common serovars detected in diarrheic foals were S. enterica subsp. enterica serovars Infantis and Minnesota. Multidrug resistance was observed in 9 of 23 isolates, with 8 of the 9 from diarrheic foals. All of the isolates contained at least 2 resistance genes, with most of the genes related to the multidrug efflux pump complex. Among the 9 isolates shown to be resistant to β-lactam antimicrobials, at least one antimicrobial resistance gene (ARG) related to the inactivation of these antimicrobials was observed (blaTEM-1, blaCMY-2, blaCTX-M-8). Among the 7 isolates resistant to the quinolone class, 3 contained ARGs (qnrB19, qnrB6). The occurrence of multidrug-resistant isolates, particularly among foals with diarrhea, and the zoonotic potential of Salmonella species, highlight the importance of implementing biosecurity measures on the studied farms.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"363-366"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessing chronic stress in cats: measuring hair cortisol using an ELISA. 评估猫的慢性应激：用ELISA法测量毛发皮质醇。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2024-12-26 DOI: 10.1177/10406387241309391

Tennille K Lamon, Jonathan Lidbury, Phillip Guadiano, Eduardo Colombo, Christine Budke

{"title":"Assessing chronic stress in cats: measuring hair cortisol using an ELISA.","authors":"Tennille K Lamon, Jonathan Lidbury, Phillip Guadiano, Eduardo Colombo, Christine Budke","doi":"10.1177/10406387241309391","DOIUrl":"10.1177/10406387241309391","url":null,"abstract":"Evaluating stress in shelter and institutionally owned cats is important to help guide improvements in their welfare. Welfare assessments often focus on behavior metrics and physiologic measurements, such as systemic cortisol levels. The gold standard for measuring acute stress is serum cortisol; measuring cortisol in feces and urine gives reliable time-integrated assessments of acute stress. Monitoring chronic stress requires using a matrix that accumulates cortisol over time, such as hair or nails. Hair was collected from 29 cats representing 2 populations: cats from a local shelter and cats owned by a university. Cortisol was extracted from the hair using a method established for extracting cortisol from bovine hair. We measured hair cortisol concentrations with a commercial ELISA that is marketed for human saliva. The mean cortisol concentration was 140 pg/mg for the shelter cats and 98 pg/mg for the university-owned cats. We found no significant difference in hair cortisol concentrations between the 2 groups (p = 0.793). The intra- and inter-assay CVs for the ELISA were 9.3% and 8.4%, respectively. Observed:expected ratios for spiking recovery and dilutional parallelism were 87.7 ± 25.8% and 99.7 ± 37.5%, respectively. Measurement of cortisol in hair samples may provide a noninvasive method to monitor chronic stress and acclimation in cats that live in confinement for prolonged periods.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"217-222"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of 3 methods of quantification of phenobarbital in canine plasma: high-performance liquid chromatography, a point-of-care immunoassay, and the FDA-approved immunoassay analyzer.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-27 DOI: 10.1177/10406387241312891

Kamoltip Thungrat, Ryan Gibson, Tom Jukier, Alex Jenkins, Crisanta Cruz-Espindola

{"title":"Comparison of 3 methods of quantification of phenobarbital in canine plasma: high-performance liquid chromatography, a point-of-care immunoassay, and the FDA-approved immunoassay analyzer.","authors":"Kamoltip Thungrat, Ryan Gibson, Tom Jukier, Alex Jenkins, Crisanta Cruz-Espindola","doi":"10.1177/10406387241312891","DOIUrl":"10.1177/10406387241312891","url":null,"abstract":"Phenobarbital is a common antiseizure medication that has a relatively narrow therapeutic window. Therapeutic drug monitoring (TDM) is a helpful tool to guide dose adjustments for phenobarbital and avoid its toxicity. We investigated the agreement among 3 methods of quantifying phenobarbital in canine plasma: high-performance liquid chromatography (HPLC), point-of-care (POC) testing, and the FDA-approved immunoassay analyzer. We randomly selected 45 plasma samples obtained by the TDM service (College of Veterinary Medicine, Auburn University, Auburn, AL, USA). Passing-Bablok regression and Lin concordance correlation coefficients (CCCs) were used to determine the agreement of the results obtained for the 3 methods; Bland-Altman plots were used for bias analysis using the results from the HPLC method as a reference. The FDA-approved immunoassay analyzer and POC immunoassay method results agreed with the HPLC. The results from the FDA-approved immunoassay analyzer were better correlated than those from the POC method, with Lin CCCs of 0.96 (95% CI: 0.93-0.98) and 0.94 (95% CI: 0.90-0.97), respectively. The average biases of the FDA-approved and the POC immunoassay analyzers were 0.80 and -0.64 µg/mL, respectively. Based on the CIs of Lin CCCs, the commercial POC phenobarbital test is a good screening tool and agrees with the HPLC method. However, the FDA-approved immunoassay analyzer method allows for more accurate quantification of phenobarbital concentrations, which is required for appropriate dose adjustment of phenobarbital.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"272-277"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypertrophic osteopathy in 4 white-tailed deer, with a literature review.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-02-05 DOI: 10.1177/10406387251318413

Alisia A W Weyna, Erin Luley, Justin D Brown, Kevin D Niedringhaus, Samantha E J Gibbs, Nicole M Nemeth

{"title":"Hypertrophic osteopathy in 4 white-tailed deer, with a literature review.","authors":"Alisia A W Weyna, Erin Luley, Justin D Brown, Kevin D Niedringhaus, Samantha E J Gibbs, Nicole M Nemeth","doi":"10.1177/10406387251318413","DOIUrl":"10.1177/10406387251318413","url":null,"abstract":"Hypertrophic osteopathy (HO) is a condition in which periosteal bone forms along long bone diaphyses and metaphyses. Lesions generally affect all 4 limbs, and often involve the distal portions. The pathogenesis is incompletely understood, and many, but not all, cases are associated with concurrent neoplasia or space-occupying masses. Among veterinary species, most cases are reported in dogs, cats, and horses, with sporadic cases in other domestic and non-domestic species. In cervids, this condition is often associated with fungal granulomas, typically in the lung. We report HO, with findings consistent to other veterinary species, in a farmed white-tailed deer (WTD) with bacterial pneumonia and in 3 free-ranging WTD, one of which had fungal pneumonia. Recognition of HO in WTD and potentially associated conditions can lead to improved sample collection in the field. Such information can improve characterization of the disease, complement our understanding of comparative pathology of this condition, and raise awareness among individuals or institutions working with cervids.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"340-344"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11803587/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Canine cystic astrocytomas: 7 cases. 犬囊性星形细胞瘤：7 例。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-27 DOI: 10.1177/10406387241312898

Vicente A Avila Reyes, Andrew D Miller, Alexander de Lahunta, Brian A Summers, Daniel R Rissi

{"title":"Canine cystic astrocytomas: 7 cases.","authors":"Vicente A Avila Reyes, Andrew D Miller, Alexander de Lahunta, Brian A Summers, Daniel R Rissi","doi":"10.1177/10406387241312898","DOIUrl":"10.1177/10406387241312898","url":null,"abstract":"Tumoral macrocysts (grossly observable cysts) are common in human pilocytic astrocytomas but are rarely reported in canine astrocytomas. Here we describe 7 canine astrocytomas with macrocysts. The median age of affected patients was 9.6 y, and 2 dogs were brachycephalic. Clinical signs reported in 6 cases included head tilt (4 cases), falling (4 cases), and ataxia (3 cases). Gross changes consisted of well (5 cases) or poorly demarcated (2 cases), tan-to-pale-yellow masses with distinct, 2-10-mm fluid-filled cysts that expanded the cerebellum (4 cases) or telencephalon (3 cases). Histology and immunohistochemistry revealed low-grade astrocytoma in 6 cases and high-grade astrocytoma in 1 case. Neoplastic cells were elongate, formed bundles embedded in an eosinophilic fibrillary stroma, and had oval-to-elongate nuclei. Gemistocytic differentiation was common (4 cases). Cystic areas were lined by neoplastic astrocytes and were either apparently empty or contained scant, eosinophilic, proteinaceous material and a few neoplastic astrocytes, foamy macrophages, and occasionally other leukocytes. The mitotic activity was absent or low across cases. Cytoplasmic immunolabeling for glial fibrillary acidic protein was detected in 6 cases. Our findings indicate that canine cystic astrocytomas are rare (our cases span several decades) and are located mainly, but not exclusively, in the cerebellum.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"398-403"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773493/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meningioma in the fourth ventricle in 2 cats. 2 只猫的第四脑室脑膜瘤。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-27 DOI: 10.1177/10406387241312895

Igor R Santos, Milena C Paz, Marcele B Bandinelli, Rochana R Fett, Daniel R Rissi, Renato S Sousa, Shannon Swist, Saulo P Pavarini

{"title":"Meningioma in the fourth ventricle in 2 cats.","authors":"Igor R Santos, Milena C Paz, Marcele B Bandinelli, Rochana R Fett, Daniel R Rissi, Renato S Sousa, Shannon Swist, Saulo P Pavarini","doi":"10.1177/10406387241312895","DOIUrl":"10.1177/10406387241312895","url":null,"abstract":"Feline meningiomas typically arise on the surface of the brain but can occur in the third ventricle. Meningiomas in the fourth ventricle have not been reported in cats, to our knowledge. Here, we describe the clinical and pathologic findings of meningioma in the fourth ventricle in 2 cats. Both affected patients (1 castrated male, 1 spayed female) were 15-y-old. Case 2 had neurologic signs, including absent menace response, head tilt, and ataxia. Grossly, both tumors were well-demarcated and firm, expanded the fourth ventricle, and compressed the adjacent neuroparenchyma. Cytology of the ventricular mass in case 1 revealed a spindle-cell neoplasm. Based on histology, the neoplasms were classified as WHO grade 1 fibrous meningioma (case 1) and transitional meningioma (case 2). Immunohistochemistry revealed diffuse, robust cytoplasmic E-cadherin and vimentin immunolabeling in both cases and multifocal, weak cytoplasmic pan-cytokeratin AE1/AE3 immunolabeling in case 2. Our findings indicate that meningiomas should be included as a differential diagnosis for tumors of the fourth ventricle in cats.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"389-392"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773495/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aortic body tumor with intracardiac metastasis in a dog. 犬主动脉体肿瘤合并心内转移一例。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1177/10406387241304438

Matthew I Crawford-Jennings, Lezith D Chavez, Emma R Loessberg, Francisco R Carvallo-Chaigneau

引用次数: 0

Intoxication of sheep by Astragalus arequipensis in northwestern Argentina.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-27 DOI: 10.1177/10406387241311815

Raul E Marin, Dale Gardner, Daniel Cook, Anibal G Armien, Renée H Fortunato, Franklin Riet-Correa, Francisco A Uzal

{"title":"Intoxication of sheep by Astragalus arequipensis in northwestern Argentina.","authors":"Raul E Marin, Dale Gardner, Daniel Cook, Anibal G Armien, Renée H Fortunato, Franklin Riet-Correa, Francisco A Uzal","doi":"10.1177/10406387241311815","DOIUrl":"10.1177/10406387241311815","url":null,"abstract":"Spontaneous intoxication by Astragalus arequipensis was diagnosed in a flock of 300 sheep in Jujuy province, northwestern Argentina, that grazed an area heavily invaded by this plant. The main clinical signs were intention tremors, ataxia, and progressive loss of condition. Autopsy of 2 affected animals revealed loss of body condition. The main microscopic changes were fine cytoplasmic vacuolation of cells in the cerebrum, cerebellum, thyroid and adrenal glands, kidney, liver, pancreas, urinary bladder, and lymph nodes, and swollen axons in the cerebellum. Ultrastructurally, the cytoplasmic vacuoles consisted of dilated secondary lysosomes. Composite leaf and stem samples of A. arequipensis analyzed by high-performance liquid chromatography contained 0.05% swainsonine. The diagnosis of intoxication by A. arequipensis was made based on the clinical history and signs; gross, microscopic, and ultrastructural changes; and detection of swainsonine in the plant.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"375-379"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11773494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143047209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro expression of genes encoding HIF1α, VEGFA, PGE2 synthases, and PGE2 receptors in feline oral squamous cell carcinoma.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-02-10 DOI: 10.1177/10406387251315677

Walaa Hamed Shaker Nasry, Juan Carlos Rodriguez-Lecompte, Chelsea K Martin

{"title":"In vitro expression of genes encoding HIF1α, VEGFA, PGE2 synthases, and PGE2 receptors in feline oral squamous cell carcinoma.","authors":"Walaa Hamed Shaker Nasry, Juan Carlos Rodriguez-Lecompte, Chelsea K Martin","doi":"10.1177/10406387251315677","DOIUrl":"10.1177/10406387251315677","url":null,"abstract":"Feline oral squamous cell carcinoma (FOSCC) is an aggressive tumor with poor outcomes. Mechanisms of prostaglandin E2 (PGE2)-related inflammation and angiogenesis interact in human OSCC; however, this relationship has not been reported in FOSCC, to our knowledge. We aimed to characterize expression of genes encoding PGE2 synthases (PTGES1-3), PGE2 receptors (EP1-4), hypoxia inducible factor 1α (HIF1A), and vascular and endothelial growth factor A (VEGFA) in FOSCC cell lines (SCCF1-3) in vitro using reverse-transcription quantitative real-time PCR (RT-qPCR). Expression of PTGES1, PTGES3, EP4, and VEGFA were serum-inducible in SCCF2 cells; VEGFA was also inducible in SCCF1 cells (p ≤ 0.05). Compared to other serum-treated cells, SCCF3 cells had the lowest VEGFA expression despite the highest HIF1A (p ≤ 0.05) expression. PGE2 (5 µg/mL and 35 µg/mL) was added to SCCF2 cells for 4 different times (30, 60, 120, 240 min). Both doses of PGE2 stimulated expression of HIF1A and CD147 at 240 min (p ≤ 0.05). PGE2 treatment stimulated cyclooxygenase 2 (COX2) expression at 30 min, followed by suppression at 60 and 120 min and a sharp reduction in EP4 expression at 60 min (p ≤ 0.05). Treatment of SCCF2 with PGE2 and EP4 antagonist L-161,982 increased COX2 expression, and L-161,982 (alone and in combination with PGE2) stimulated EP4 expression (p ≤ 0.05). Genes for PGE2 synthase enzymes, PGE2 receptors, HIF1α and VEGFA were expressed in FOSCC cells in vitro. SCCF2 cells responded to exogenous PGE2 and EP4 antagonism, suggesting that EP4 activity in FOSCC deserves more study.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"223-233"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11811947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to: Oxytetracycline-resistant Paenibacillus larvae identified in commercial beekeeping operations in Saskatchewan using pooled honey sampling. 勘误表：在萨斯喀彻温省的商业养蜂业中使用混合蜂蜜取样鉴定出具有土霉素抗性的芽孢杆菌幼虫。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-10 DOI: 10.1177/10406387241308319

引用次数: 0