Journal of Veterinary Diagnostic Investigation最新文献_第3页

Anogenital papillomatosis associated with a novel papillomavirus in a grey-headed flying fox. 灰头狐的肛门生殖器乳头状瘤病与一种新型乳头状瘤病毒有关。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-05-24 DOI: 10.1177/10406387251341935

John S Munday, Susan E Brown, Emily E Kay, Ludovica D Valenza

{"title":"Anogenital papillomatosis associated with a novel papillomavirus in a grey-headed flying fox.","authors":"John S Munday, Susan E Brown, Emily E Kay, Ludovica D Valenza","doi":"10.1177/10406387251341935","DOIUrl":"10.1177/10406387251341935","url":null,"abstract":"A 0.5-cm nodular thickening of the vagina was observed in a flying fox (Pteropus poliocephalus) that had been rescued after becoming entangled in fruit netting. Over the following 6 mo, the thickening progressed to diffuse multinodular mucosal thickening of both the vagina and anus. The proliferative lesions were removed surgically. Histologically, the thickened mucosa was arranged in numerous small exophytic papillomas. Cells within the basilar layers were crowded and basophilic. Rarely, enlarged cells that contained increased quantities of pale, smudged eosinophilic cytoplasm [consistent with papillomavirus (PV)-induced cellular changes] were visible. PCR amplified a PV DNA sequence from a sample of affected vaginal mucosa; the sequence was ~90% similar to a PV DNA sequence previously detected as a subclinical infection in an African species of fruit bat. The lesion had not recurred within 6 mo of surgical excision. This is the second report of PV-associated disease in bats; to our knowledge, PV infection has not been reported previously in association with anogenital lesions in bats. Additionally, to our knowledge, a PV has not been identified previously in an Australian bat species.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"833-836"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient blocking ELISA for bovine alphaherpesvirus 1 using a gB epitope-specific monoclonal antibody. 使用gB表位特异性单克隆抗体高效阻断牛α疱疹病毒1的ELISA试剂盒。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-06-18 DOI: 10.1177/10406387251346909

Shuang-Yan Xiao, Feng-Dong Zhu, Dan-Jing Wang, Yan-Long Hu, Hui-Qin Jia, Huan-Chun Chen, Zheng-Fei Liu

{"title":"Efficient blocking ELISA for bovine alphaherpesvirus 1 using a gB epitope-specific monoclonal antibody.","authors":"Shuang-Yan Xiao, Feng-Dong Zhu, Dan-Jing Wang, Yan-Long Hu, Hui-Qin Jia, Huan-Chun Chen, Zheng-Fei Liu","doi":"10.1177/10406387251346909","DOIUrl":"10.1177/10406387251346909","url":null,"abstract":"Infectious bovine rhinotracheitis (IBR) is an infectious respiratory disease in cattle that is caused by bovine alphaherpesvirus 1 (BoAHV1). We immunized BALB/c mice with inactivated and purified BoAHV1 to prepare hybridoma cells. After the successful establishment of a positive hybridoma cell line, co-immunoprecipitation coupled with mass spectrometry unveiled the predominant targeting of glycoprotein B (gB) by the hybridoma cells. Through bioinformatics analysis and Western blot techniques, we identified the epitope of the monoclonal antibody (mAb) against gB to amino acids 1-170. Subsequently, the 1H3 mAb was leveraged for the development of a gB blocking ELISA (gB-bELISA), utilizing inactivated BoAHV1 virions as the coating antigen. The optimized protocol involved diluting samples 2-fold with 1% fish gelatin, followed by incubation periods of 120 min for samples, 30 min for HRP-conjugated 1H3 mAb, and 15 min for the TMB substrate. We validated our assay using 268 bovine serum samples with clear backgrounds and established the cutoff value of 43.8% through ROC analysis. Additionally, we tested 256 clinical bovine serum samples using both our gB-bELISA and a virus neutralization test, achieving a concordance rate of 95.3%. Based on testing 495 randomly selected sera from 18 counties for BoAHV1 antibodies with our gB-bELISA, the seroprevalence of IBR in the Central China region was 22.0% (95% CI: 18.4, 25.7). Our gB-bELISA could be a valuable tool for the clinical detection of IBR, supporting disease control and eradication efforts.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"786-799"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An investigation of the role of wild rats in transmitting Leptospira spp. to stray cats and dogs in Malaysia. 马来西亚野鼠传播钩端螺旋体给流浪猫和狗的调查。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-07-16 DOI: 10.1177/10406387251355254

Zafirah Muhd, Kuan Hua Khor, Abdul Rahman Alashraf, Seng Fong Lau, Rozanaliza Radzi, Siti Khairani Bejo, Rohaidah Hashim

{"title":"An investigation of the role of wild rats in transmitting Leptospira spp. to stray cats and dogs in Malaysia.","authors":"Zafirah Muhd, Kuan Hua Khor, Abdul Rahman Alashraf, Seng Fong Lau, Rozanaliza Radzi, Siti Khairani Bejo, Rohaidah Hashim","doi":"10.1177/10406387251355254","DOIUrl":"10.1177/10406387251355254","url":null,"abstract":"Stray cats and dogs have been reported to shed Leptospira spp., and wild rats are speculated to be involved. We aimed to elucidate the role of wild rats in transmitting Leptospira to stray cats and dogs in Malaysia. We tested sera from 124 wild rats with the microscopic agglutination test (MAT): 88 of 122 (72%) sera were positive (titer ≥1:100), with the predominant serovars Icterohaemorrhagiae, Bataviae, Ballum, Javanica, Lai, and Pomona. With a Leptospira-specific PCR assay, we detected pathogenic Leptospira spp. in 33 of 124 (27%) kidney samples and 13 of 79 (16%) urine samples. Isolates obtained by culture of rat kidney and urine were identified to the species level with MAT using hyperimmune sera and the PCR assay. From 29 isolates, 2 pathogenic species were identified: L. interrogans serovar Bataviae and L. borgpetersenii serovar Javanica. Phylogenetic analysis using partial 16S rDNA sequences of the Leptospira spp. from the wild rats indicated that the species were similar to isolates from stray cats and dogs in previous studies. We confirmed that wild rats carried pathogenic Leptospira spp. and were a potential source of leptospiral infection of stray cats and dogs in Malaysia.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"800-805"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and validation of ELISAs for interferon-gamma determination in bison. 野牛血清干扰素测定elisa的优化与验证。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-06-03 DOI: 10.1177/10406387251344567

Josephine Chileshe, Todd Shury, Jeffrey M Chen

{"title":"Optimization and validation of ELISAs for interferon-gamma determination in bison.","authors":"Josephine Chileshe, Todd Shury, Jeffrey M Chen","doi":"10.1177/10406387251344567","DOIUrl":"10.1177/10406387251344567","url":null,"abstract":"Bovine tuberculosis, caused by Mycobacterium bovis, is endemic in the Wood Buffalo National Park, Canada, home to free-ranging and threatened wood bison. This disease poses a threat to the conservation of this culturally and ecologically important animal species, as well as potentially impacting the health of humans and other animal species via zoonosis and spillover, respectively. The ability to detect infection early will minimize and prevent the potential risk of M. bovis transmission. Interferon-gamma (IFNγ) assays are a reliable detection method for M. bovis in cattle and other wildlife species and may have diagnostic value in bison as well. We aimed to optimize and partially validate 2 commercial IFNγ ELISAs to detect endogenous bison IFNγ in mitogen-stimulated whole blood. Parameters evaluated included antibody identification, sample matrix effect, dilution linearity, assay reproducibility, and limit of quantification. The optimized assays demonstrated linear responses to recombinant bovine and endogenous bison IFNγ (range: 1-125 pg/mL; R2 = 0.99), with good recovery and fair reproducibility, and a low limit of quantification of 1 pg/mL. Mabtech bovine Flex and Pro kits have the same antibodies but in 2 different assay formats; an in-house assay platform (Flex kit) and precoated plates (Pro kit) are considered suitable for measuring bison IFNγ, offering flexibility depending on available resources.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"764-770"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12133778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lack of serologic evidence of orthoflavivirus infection in dogs with meningoencephalitis of unknown origin and steroid-responsive meningitis-arteritis in the Netherlands. 荷兰不明原因脑膜脑炎和类固醇反应性脑膜炎动脉炎犬中正黄病毒感染的血清学证据缺乏。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-05-21 DOI: 10.1177/10406387251340619

Koen M Santifort, Kiki Streng, Niklas Bergknut, Iris Van Soens, Marta Plonek, Wim H M van der Poel

{"title":"Lack of serologic evidence of orthoflavivirus infection in dogs with meningoencephalitis of unknown origin and steroid-responsive meningitis-arteritis in the Netherlands.","authors":"Koen M Santifort, Kiki Streng, Niklas Bergknut, Iris Van Soens, Marta Plonek, Wim H M van der Poel","doi":"10.1177/10406387251340619","DOIUrl":"10.1177/10406387251340619","url":null,"abstract":"The pathogenesis of meningoencephalomyelitis of unknown origin (MUO) and steroid-responsive meningitis-arteritis (SRMA) in dogs remains enigmatic. Numerous studies have attempted and failed to identify (viral) pathogens in samples from MUO- or SRMA-diagnosed dogs. Orthoflavivirus-associated meningoencephalitis or meningoencephalomyelitis has been diagnosed in dogs in several European countries. We investigated serologic evidence for orthoflavivirus infection in dogs with clinical diagnoses of MUO or SRMA in the Netherlands. Twelve dogs with a clinical diagnosis of MUO based on signalment, neurologic examination, MRI studies, CSF analysis, and response to treatment were included in the study (age range: 1-11 y; 4 females, 8 males; weight range: 8-44 kg). Serum samples from all 12 dogs tested negative in a commercial competitive ELISA and virus neutralization tests for West Nile virus, Usutu virus, and tick-borne encephalitis virus. We did not find serologic evidence of orthoflavivirus infection in dogs with MUO or SRMA in the Netherlands.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"817-821"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A retrospective study of benign epithelial salivary gland neoplasia in 11 dogs and 5 cats. 11只狗和5只猫涎腺良性上皮瘤的回顾性研究。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-06-12 DOI: 10.1177/10406387251329526

Daniel Felipe Barrantes Murillo, Emily J Brinker, Daniel R Rissi, Brian F Porter, Tatiane Terumi Negrão Watanabe

{"title":"A retrospective study of benign epithelial salivary gland neoplasia in 11 dogs and 5 cats.","authors":"Daniel Felipe Barrantes Murillo, Emily J Brinker, Daniel R Rissi, Brian F Porter, Tatiane Terumi Negrão Watanabe","doi":"10.1177/10406387251329526","DOIUrl":"10.1177/10406387251329526","url":null,"abstract":"Benign salivary gland neoplasms are infrequent in dogs and cats. The available literature is scarce, relying on scattered case series and case reports. The histologic subclassification of these neoplasms in humans is correlated with tumor outcome and is used as a template for the veterinary species. To better describe the clinical and histologic features of these benign epithelial tumors in dogs and cats, we performed a retrospective multi-institutional study and retrieved 16 benign epithelial salivary neoplasms from 11 dogs and 5 cats. In dogs, the sex distribution was 7 castrated males, 3 spayed females, and 1 intact female. The average age at diagnosis was 10.4 y (range: 4-15 y; ±3.0 SD). The diagnoses were canalicular adenoma (5 cases), pleomorphic adenoma (3 cases), cystadenoma (2 cases), and basal cell adenoma (1 case). In cats, the sex distribution was 4 spayed females and 1 castrated male. The average age at diagnosis was 11 y (range: 7-18 y; ±4.6 SD). Diagnoses were canalicular adenoma (4 cases) and cystadenoma (1 case). The diagnostic pathologist should be familiar with the current veterinary and human nomenclature of these neoplasms to make an accurate diagnosis.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"806-812"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCR-RFLP assays to detect recessive lethal alleles in Landrace and Duroc pigs in Vietnam. PCR-RFLP检测越南长白猪和杜洛克猪隐性致死等位基因。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-08-07 DOI: 10.1177/10406387251318022

Anh Phu Nam Bui, Nghiep Mai Nguyen, Quang Le

引用次数: 0

Diseases of the nervous system of equids in Brazil: a review. 巴西马科动物神经系统疾病综述。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-05-30 DOI: 10.1177/10406387251325881

Kesia S Carvalho, Claudio S L de Barros, Fabio S Mendonça, Mizael Machado, Franklin Riet-Correa

{"title":"Diseases of the nervous system of equids in Brazil: a review.","authors":"Kesia S Carvalho, Claudio S L de Barros, Fabio S Mendonça, Mizael Machado, Franklin Riet-Correa","doi":"10.1177/10406387251325881","DOIUrl":"10.1177/10406387251325881","url":null,"abstract":"In Brazil, CNS diseases in equids were little known until the 1980s. Since then, several diagnostic laboratories have been operating in different universities, initially in the South and Southeast regions and, later, in the Central-West, Northeast and North regions. However, the knowledge accumulated from the diagnoses of nervous system diseases of equids made at these institutions over the years has not been reviewed comprehensively, and many papers are published in Portuguese. Here, we review 18 diseases that occur in the nervous system of equids in Brazil, including some critical infectious diseases (rabies, equine encephalomyelitides, equine herpesviral myeloencephalopathy, protozoan myeloencephalitis), and diseases caused by toxic plants and mycotoxins. Our review provides reference data to assist pathologists and clinicians in diagnosing neurologic diseases in equids. We also hope it will serve as a reference for professionals in this field abroad, allowing them to compare data in the epidemiology and pathology of the nervous system diseases of equids in different regions of the world.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"729-752"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxalate nephropathy in cattle associated with the consumption of Megathyrsus maximus in Argentina. 草酸盐肾病在牛与阿根廷大鳄消费有关。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-05-19 DOI: 10.1177/10406387251336265

Agustín Avellaneda-Cáceres, Stephen T Lee, Álvaro Ruiz, Gabriela V Sandoval, Luis A Colque-Caro, Daniel Cook, Laura S Aguirre, Francisco A Uzal, Juan F Micheloud

{"title":"Oxalate nephropathy in cattle associated with the consumption of Megathyrsus maximus in Argentina.","authors":"Agustín Avellaneda-Cáceres, Stephen T Lee, Álvaro Ruiz, Gabriela V Sandoval, Luis A Colque-Caro, Daniel Cook, Laura S Aguirre, Francisco A Uzal, Juan F Micheloud","doi":"10.1177/10406387251336265","DOIUrl":"10.1177/10406387251336265","url":null,"abstract":"Over a 2-wk period, 20 cows that were grazing a Megathyrsus maximus (Guinea grass) pasture died after developing depression, respiratory difficulty, and recumbency. Affected animals had increased serum urea, phosphorus, and creatinine concentrations, and below-normal calcium concentrations. Autopsy revealed moderate ascites; mildly enlarged, pale, mottled kidneys; and perirenal edema. Microscopically, there was hyperplasia of mesangial cells in the renal glomeruli, renal tubular epithelial necrosis, and hyaline casts and refractive crystals in cortical and medullary renal tubular lumens. Samples of M. maximus from the affected pasture contained elevated concentrations of soluble oxalate (3.71%). Our findings suggest that oxalate produced by M. maximus caused acute tubular injury. Our case highlights the critical need for monitoring oxalate levels in pastures and managing grazing practices, particularly under drought conditions, to prevent similar outbreaks in the future.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"813-816"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144094155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing detection of porcine reproductive and respiratory syndrome virus RNA in processing fluids stored at room temperature using PrimeStore molecular transport medium. 利用PrimeStore分子运输介质提高对猪繁殖与呼吸综合征病毒RNA的检测。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-06-12 DOI: 10.1177/10406387251348464

Onyekachukwu Osemeke, Jerry Torrison, Christopher Helm, Michael Zeller, Rabsa Naseer, Claire Le Fevre, Grant Allison, James Mark Hammer, Giovani Trevisan, Phillip Gauger, Daniel Linhares

{"title":"Enhancing detection of porcine reproductive and respiratory syndrome virus RNA in processing fluids stored at room temperature using PrimeStore molecular transport medium.","authors":"Onyekachukwu Osemeke, Jerry Torrison, Christopher Helm, Michael Zeller, Rabsa Naseer, Claire Le Fevre, Grant Allison, James Mark Hammer, Giovani Trevisan, Phillip Gauger, Daniel Linhares","doi":"10.1177/10406387251348464","DOIUrl":"10.1177/10406387251348464","url":null,"abstract":"Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen affecting the U.S. swine industry, resulting in substantial costs associated with disease prevention, elimination, and control. Processing fluids (PFs) are an increasingly popular sample type for PRRSV surveillance due to their cost-effectiveness and high herd-level sensitivity. However, like other aggregate sample types, PF samples are non-pristine, and the RNA within them is susceptible to degradation, increasing the risk of false-negative and/or inconsistent results in reverse-transcription real-time PCR (RT-rtPCR) testing. We evaluated the efficacy of the nucleic acid-preserving reagent PrimeStore molecular transport medium (PS-MTM) in improving PRRSV RT-rtPCR detection rates, Ct values, and whole-genome sequencing (WGS) outcomes in PF samples. We collected PF samples from 2 PRRSV-positive sow herds and divided them into untreated controls and 2 PS-MTM-treated groups. PS-MTM-treated PFs had significantly lower Ct values compared to untreated samples, indicating improved RNA preservation and detectability. Although sequencing outcomes varied between study groups, PS-MTM-treated samples had statistically higher unique read counts. However, differences in other measured sequencing outcomes between the study groups were not statistically significant. PS-MTM has potential in PF sampling protocols to enhance PRRSV surveillance accuracy by reducing false-negative RT-rtPCR results.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"822-827"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0