Journal of Veterinary Diagnostic Investigation最新文献_第3页

Comparative analysis of 3 qPCR primer-probe sets for the detection of equid alphaherpesvirus 1. 3种qPCR引物-探针检测马α疱疹病毒1型的比较分析。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-10-07 DOI: 10.1177/10406387251379857

Yoshinori Kambayashi, Hiroshi Bannai, Manabu Nemoto, Nanako Kawanishi, Hidekazu Niwa, Koji Tsujimura

{"title":"Comparative analysis of 3 qPCR primer-probe sets for the detection of equid alphaherpesvirus 1.","authors":"Yoshinori Kambayashi, Hiroshi Bannai, Manabu Nemoto, Nanako Kawanishi, Hidekazu Niwa, Koji Tsujimura","doi":"10.1177/10406387251379857","DOIUrl":"10.1177/10406387251379857","url":null,"abstract":"With the revision of the World Organisation for Animal Health (WOAH) Terrestrial Manual on equine rhinopneumonitis in 2024, 3 recommended qPCR primer-probe sets were added for the detection of equid alphaherpesvirus 1 (EqAHV1; formerly equine herpesvirus 1 [EHV1]; family Orthoherpesviridae, taxon species Varicellovirus equidalpha1), also known as equine abortion virus. We compared the sensitivity and specificity of the 3 qPCR primer-probe sets to determine the most reliable set. Sets gB1H and gB1P, which target the glycoprotein B (gB) gene of EqAHV1, detected all 10 copies and even lower copy numbers. In contrast, set gC1 (ISO 17025-accredited method used at the WOAH reference laboratory), which targets the glycoprotein C (gC) gene, failed to detect ≤10 copies of EqAHV1. Our results showed the lower sensitivity of gC1, which was not improved by modification of primer and probe concentrations. gB1P detected not only EqAHV1 but also equid alphaherpesvirus 4 (EqAHV4; Orthoherpesviridae, Varicellovirus equidalpha4), likely owing to an erroneous amplification of the homologous EqAHV4 gB gene, indicating that gB1P is not suitable for the detection of EqAHV1 with high specificity. We then compared gB1H with gB1D, a set recommended in the previous version of the Manual, using 120 nasal swabs collected from febrile horses. gB1H had slightly higher sensitivity than gB1D. gB1H proved to be the most reliable primer-probe set for detecting EqAHV1, with high sensitivity and specificity. Nevertheless, individual laboratories are encouraged to validate these methods under their own conditions before implementation.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251379857"},"PeriodicalIF":1.1,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12504209/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145238875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selected microRNAs as biomarkers in sarcoid-affected horses under immunotherapy with a mistletoe extract. 槲寄生提取物免疫治疗下瘤样病变马的选定microrna作为生物标志物。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-10-03 DOI: 10.1177/10406387251362820

Anke Beermann, Eman Hamza, Sonja Reinhard, Christoph Koch, Thomas Oberhänsli, Lucia Unger

{"title":"Selected microRNAs as biomarkers in sarcoid-affected horses under immunotherapy with a mistletoe extract.","authors":"Anke Beermann, Eman Hamza, Sonja Reinhard, Christoph Koch, Thomas Oberhänsli, Lucia Unger","doi":"10.1177/10406387251362820","DOIUrl":"10.1177/10406387251362820","url":null,"abstract":"We investigated microRNAs (miRNAs) as potential prognostic biomarkers for equine sarcoid (ES) disease. In a breed-, age-, and sex-matched case-controlled study involving 45 ES-affected and 15 control horses, we assessed the diagnostic, prognostic, and theragnostic value of 3 miRNAs (eca-miR-127, eca-miR-379, eca-miR-432) in horses treated with European mistletoe (Viscum album) extract versus placebo. Whole-blood miRNA concentrations were measured using reverse-transcription quantitative real-time PCR (RT-qPCR) at 3 different times. We found that eca-miR-432 expression was lower in ES-affected (median = -1.93; 95% CI: -2.03 to -.86) compared to control (median = -1.71; 95% CI: -1.92 to -1.6) horses (p = 0.03, r = 0.3; 95% CI: 0.024-0.57) with a median difference of -1.93 versus -1.71, respectively. The ROC curve analysis indicated an area under the curve of 0.71 (95% CI: 0.51-0.84; p = 0.005) with a sensitivity of 74% (95% CI: 61-88%) and a specificity of 73% (95% CI: 39-94%) to diagnose ES. However, none of the miRNAs evaluated had prognostic potential or significant changes in expression following treatment. Additionally, miRNA expression was not influenced by breed, sex, or season. Although whole-blood eca-miR-432 had moderate diagnostic potential for ES, identifying prognostic miRNA biomarkers for ES remains a challenge.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251362820"},"PeriodicalIF":1.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12494582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ectopic splenic tissue in 46 dogs, 2000-2024. 脾组织异位46只犬，2000-2024。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-10-03 DOI: 10.1177/10406387251379922

C Robert Stilz, Ricardo E Mendes, Claudio S L Barros, Daniel R Rissi

{"title":"Ectopic splenic tissue in 46 dogs, 2000-2024.","authors":"C Robert Stilz, Ricardo E Mendes, Claudio S L Barros, Daniel R Rissi","doi":"10.1177/10406387251379922","DOIUrl":"10.1177/10406387251379922","url":null,"abstract":"Ectopic splenic tissue (accessory spleen or splenosis) occurs as dark-red-to-brown or purple nodules outside the spleen. Accessory spleens are congenital lesions histologically identical to a normal spleen. Splenosis results from implantation of splenic tissue following splenic rupture and lacks features of normal spleen. However, these distinctions have been largely applied to human cases, and the terms are often used interchangeably in domestic animals. Here we describe ectopic splenic tissue in 46 canine surgical biopsy specimens examined at the Athens Veterinary Diagnostic Laboratory, 2000-2024. The omentum (39 cases) and mesentery (5) were the most commonly affected sites. Original diagnoses were accessory spleen (28 cases), splenosis (14), accessory spleen or splenosis (2), and ectopic splenic tissue and normal splenic tissue (1 each). Updated diagnoses, modified after histologic assessment for a fibrous capsule, smooth muscle trabeculae, and white and red pulp, were accessory spleen (37 cases) and splenosis (9). Concurrent splenic lesions were reported in 12 cases in which accessory spleens were diagnosed and only 2 splenosis cases, confirming that the histologic diagnosis of accessory spleen and splenosis is not always correlated with the clinical history and gross findings (no splenic lesions vs. splenic lesions with rupture). For that reason, ectopic splenic tissue may be a more inclusive and better term for these lesions. Hemangiosarcoma was diagnosed in the spleen in 4 of the 12 cases with splenic masses, which underscores the importance of the differentiation between ectopic splenic tissue and hemangiosarcoma.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251379922"},"PeriodicalIF":1.1,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12494584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145213090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High mortality in a commercial turkey flock associated with coinfection by Pasteurella multocida and Mycoplasmoides (Mycoplasma) gallisepticum. 多杀性巴氏杆菌和鸡败支原体共同感染导致商品火鸡群高死亡率。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-25 DOI: 10.1177/10406387251378687

Carlos Daniel Gornatti-Churria, Carmen Jerry, Shayne Ramsubeik, Mark Bland, Francisco A Uzal, Tiffany Santoro, Simone T Stoute

{"title":"High mortality in a commercial turkey flock associated with coinfection by Pasteurella multocida and Mycoplasmoides (Mycoplasma) gallisepticum.","authors":"Carlos Daniel Gornatti-Churria, Carmen Jerry, Shayne Ramsubeik, Mark Bland, Francisco A Uzal, Tiffany Santoro, Simone T Stoute","doi":"10.1177/10406387251378687","DOIUrl":"10.1177/10406387251378687","url":null,"abstract":"Six 11-wk-old, commercial, Broad-Breasted White, meat turkeys were submitted to the Turlock branch of the California Animal Health & Food Safety (CAHFS) laboratory for autopsy and diagnostic work-up. Clinical signs in the turkeys of the affected flock included depression, ruffled feathers, swollen periorbital areas, rales, and sneezing. A mortality of 50% (5,000 of 10,000) was reported at the time of case submission. Flock morbidity was 100% by 12 wk of age, and mortality eventually exceeded 90%. Fibrinous pleuropneumonia, airsacculitis, increased luminal mucoid exudate in the nasal cavities and tracheas, mottled and enlarged spleens, and hepatomegaly were the most remarkable gross findings. Microscopically, fibrinoheterophilic pneumonia and epicarditis with intralesional bacterial colonies, and necrotizing hepatitis and splenitis, were noted. Mycoplasmoides (Mycoplasma) gallisepticum (MG) was detected in tracheal and sinus pools by quantitative real-time PCR. Multilocus sequence analysis of the mgc2 gene and IGSR segment of MG differentiated our strain from MG vaccine strains, but were similar to MG isolates detected previously in other commercial turkey operations in California. Pasteurella multocida was isolated from air sacs, lungs, tracheas, hearts, and livers, and classified as profile HhaI 0001, strain X-73, by restriction enzyme analysis DNA fingerprinting. Coinfection with P. multocida and MG in a susceptible flock resulted in rapid elevation of mortality and significant economic losses in this commercial meat turkey operation.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251378687"},"PeriodicalIF":1.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pineal parenchymal tumor in a domestic rabbit. 家兔松果体实质肿瘤。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-25 DOI: 10.1177/10406387251371014

Katti R Crakes, Charles G Eberhart, John A Flanders, John G Trupkiewicz

{"title":"Pineal parenchymal tumor in a domestic rabbit.","authors":"Katti R Crakes, Charles G Eberhart, John A Flanders, John G Trupkiewicz","doi":"10.1177/10406387251371014","DOIUrl":"10.1177/10406387251371014","url":null,"abstract":"An 11-y-old male lionhead rabbit (Oryctolagus cuniculus) was presented with progressive hindlimb weakness and right-sided neurologic deficits, and was subsequently euthanized due to poor prognosis. Autopsy revealed a 1.6 × 1.1 × 1.0-cm, well-circumscribed, extra-axial mass compressing the occipital lobe and affecting both telencephalic hemispheres. Histologic and immunohistochemical analyses demonstrating positivity for synaptophysin and neuron-specific enolase, along with a Ki67 proliferative index of ~20%, were highly suggestive of a high-grade pineal parenchymal tumor (PPT). The tumor was densely cellular with marked atypia and frequent binucleation, and lacked pineocytomatous rosettes-features most consistent with a pineal parenchymal tumor of intermediate differentiation in humans. No evidence of metastasis was observed. Pineal tumors are exceptionally rare in domestic animals, with limited documentation in species such as dogs, horses, goats, cattle, and birds. To our knowledge, PPT has not been reported previously in a rabbit, underscoring the diagnostic challenges associated with intracranial neoplasms in this species.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251371014"},"PeriodicalIF":1.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A retrospective study of 171 cases of equine meningoencephalomyelitis in the United States, 1996-2023. 1996-2023年美国171例马脑膜脊髓炎的回顾性研究

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-23 DOI: 10.1177/10406387251362241

Kerstyn Countrymann, Rebecca Ruby, Andrew D Miller

{"title":"A retrospective study of 171 cases of equine meningoencephalomyelitis in the United States, 1996-2023.","authors":"Kerstyn Countrymann, Rebecca Ruby, Andrew D Miller","doi":"10.1177/10406387251362241","DOIUrl":"10.1177/10406387251362241","url":null,"abstract":"Equine meningoencephalomyelitis is an important cause of morbidity and mortality and is associated with a wide variety of infectious etiologies. Because of the lack of large retrospective studies, the prevalence and incidence of these diseases are unknown. Here we describe 171 cases of meningoencephalomyelitis in horses submitted to the Section of Anatomic Pathology at the New York State Animal Health Diagnostic Center (Cornell University, Ithaca, NY, USA) from 1996-2023. Neuroinflammatory disease was identified in 5.4% of submitted horses with a wide breed, age, and sex distribution. A parasitic cause was identified in 32 (19%) cases, with protozoa in 18 (11%) cases and metazoa in 14 (8%) cases. A viral cause was identified in 31 (18%) cases, corresponding to infection by equid alphaherpesvirus 1 (EqAHV1; 12 of 31, 39%), eastern equine encephalitis virus (10 of 31; 32%), West Nile virus (5 of 31; 16%), and rabies virus (4 of 31; 13%), followed by 14 bacterial (8%) cases and 7 fungal (4%) cases. Of the remaining 87 of 171 (51%) cases, 20 (23%) had some histologic features, although not conclusive, of protozoal disease, and 8 (9%) of EqAHV1 infection. However, 59 (68%) cases did not have any neuropathologic changes that would support a definitive diagnosis. Although we found the expected causes of equine meningoencephalomyelitis in our study, the large number of cases with unknown etiologic diagnoses highlights the challenges of definitively proving causes of neuroinflammation in the horse and supports the need for improved ante- and postmortem testing.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251362241"},"PeriodicalIF":1.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12460270/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perirenal hemorrhage associated with feline infectious peritonitis: a novel presentation of a classic disease. 与猫感染性腹膜炎相关的肾周出血：一种经典疾病的新表现。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-05-29 DOI: 10.1177/10406387251341239

Madeleine I Gauthier, Carolyn Legge, Dayna A Goldsmith, Maria Bravo Araya, Jennifer L Davies

{"title":"Perirenal hemorrhage associated with feline infectious peritonitis: a novel presentation of a classic disease.","authors":"Madeleine I Gauthier, Carolyn Legge, Dayna A Goldsmith, Maria Bravo Araya, Jennifer L Davies","doi":"10.1177/10406387251341239","DOIUrl":"10.1177/10406387251341239","url":null,"abstract":"Feline infectious peritonitis (FIP), caused by a mutated biotype of feline coronavirus (FCoV; Coronaviridae, Alphacoronavirus), is a significant disease of felids. We investigated perirenal hemorrhage, an unreported lesion in FIP, through a retrospective analysis of 51 immunohistochemistry-confirmed FIP cases submitted to the Diagnostic Services Unit (DSU; University of Calgary, Calgary, Alberta, Canada) between 2010 June 30 and 2024 June 30. Five cats had perirenal hemorrhage in the right retroperitoneal space; 4 had concurrent subcapsular renal hemorrhage; and 1 had sublumbar muscle hemorrhage and hemoabdomen. One case had additional hemorrhages in the brain and cervical spinal cord. Concurrent gross lesions typical of FIP included pyogranulomatous inflammation in various organs and protein-rich cavitary effusions. Histologic lesions typical of FIP (vasculitis and pyogranulomatous inflammation) were present in the kidneys and retroperitoneal fat of 4 cases, and in 3 cases, FCoV antigen was demonstrated in the regions of hemorrhage. The exact mechanism of this hemorrhage is unknown, but we speculate that vasculitis caused by FIP is the cause. Despite the relatively low prevalence of perirenal hemorrhage in this cohort, this lesion represents a unique, previously unreported manifestation of FIP that clinicians and pathologists should be aware of and consider in the differential diagnosis for fluid accumulation or space-occupying lesions in the retroperitoneum of cats.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"828-832"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anogenital papillomatosis associated with a novel papillomavirus in a grey-headed flying fox. 灰头狐的肛门生殖器乳头状瘤病与一种新型乳头状瘤病毒有关。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-05-24 DOI: 10.1177/10406387251341935

John S Munday, Susan E Brown, Emily E Kay, Ludovica D Valenza

{"title":"Anogenital papillomatosis associated with a novel papillomavirus in a grey-headed flying fox.","authors":"John S Munday, Susan E Brown, Emily E Kay, Ludovica D Valenza","doi":"10.1177/10406387251341935","DOIUrl":"10.1177/10406387251341935","url":null,"abstract":"A 0.5-cm nodular thickening of the vagina was observed in a flying fox (Pteropus poliocephalus) that had been rescued after becoming entangled in fruit netting. Over the following 6 mo, the thickening progressed to diffuse multinodular mucosal thickening of both the vagina and anus. The proliferative lesions were removed surgically. Histologically, the thickened mucosa was arranged in numerous small exophytic papillomas. Cells within the basilar layers were crowded and basophilic. Rarely, enlarged cells that contained increased quantities of pale, smudged eosinophilic cytoplasm [consistent with papillomavirus (PV)-induced cellular changes] were visible. PCR amplified a PV DNA sequence from a sample of affected vaginal mucosa; the sequence was ~90% similar to a PV DNA sequence previously detected as a subclinical infection in an African species of fruit bat. The lesion had not recurred within 6 mo of surgical excision. This is the second report of PV-associated disease in bats; to our knowledge, PV infection has not been reported previously in association with anogenital lesions in bats. Additionally, to our knowledge, a PV has not been identified previously in an Australian bat species.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"833-836"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12103461/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient blocking ELISA for bovine alphaherpesvirus 1 using a gB epitope-specific monoclonal antibody. 使用gB表位特异性单克隆抗体高效阻断牛α疱疹病毒1的ELISA试剂盒。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-06-18 DOI: 10.1177/10406387251346909

Shuang-Yan Xiao, Feng-Dong Zhu, Dan-Jing Wang, Yan-Long Hu, Hui-Qin Jia, Huan-Chun Chen, Zheng-Fei Liu

{"title":"Efficient blocking ELISA for bovine alphaherpesvirus 1 using a gB epitope-specific monoclonal antibody.","authors":"Shuang-Yan Xiao, Feng-Dong Zhu, Dan-Jing Wang, Yan-Long Hu, Hui-Qin Jia, Huan-Chun Chen, Zheng-Fei Liu","doi":"10.1177/10406387251346909","DOIUrl":"10.1177/10406387251346909","url":null,"abstract":"Infectious bovine rhinotracheitis (IBR) is an infectious respiratory disease in cattle that is caused by bovine alphaherpesvirus 1 (BoAHV1). We immunized BALB/c mice with inactivated and purified BoAHV1 to prepare hybridoma cells. After the successful establishment of a positive hybridoma cell line, co-immunoprecipitation coupled with mass spectrometry unveiled the predominant targeting of glycoprotein B (gB) by the hybridoma cells. Through bioinformatics analysis and Western blot techniques, we identified the epitope of the monoclonal antibody (mAb) against gB to amino acids 1-170. Subsequently, the 1H3 mAb was leveraged for the development of a gB blocking ELISA (gB-bELISA), utilizing inactivated BoAHV1 virions as the coating antigen. The optimized protocol involved diluting samples 2-fold with 1% fish gelatin, followed by incubation periods of 120 min for samples, 30 min for HRP-conjugated 1H3 mAb, and 15 min for the TMB substrate. We validated our assay using 268 bovine serum samples with clear backgrounds and established the cutoff value of 43.8% through ROC analysis. Additionally, we tested 256 clinical bovine serum samples using both our gB-bELISA and a virus neutralization test, achieving a concordance rate of 95.3%. Based on testing 495 randomly selected sera from 18 counties for BoAHV1 antibodies with our gB-bELISA, the seroprevalence of IBR in the Central China region was 22.0% (95% CI: 18.4, 25.7). Our gB-bELISA could be a valuable tool for the clinical detection of IBR, supporting disease control and eradication efforts.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"786-799"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An investigation of the role of wild rats in transmitting Leptospira spp. to stray cats and dogs in Malaysia. 马来西亚野鼠传播钩端螺旋体给流浪猫和狗的调查。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-07-16 DOI: 10.1177/10406387251355254

Zafirah Muhd, Kuan Hua Khor, Abdul Rahman Alashraf, Seng Fong Lau, Rozanaliza Radzi, Siti Khairani Bejo, Rohaidah Hashim

{"title":"An investigation of the role of wild rats in transmitting Leptospira spp. to stray cats and dogs in Malaysia.","authors":"Zafirah Muhd, Kuan Hua Khor, Abdul Rahman Alashraf, Seng Fong Lau, Rozanaliza Radzi, Siti Khairani Bejo, Rohaidah Hashim","doi":"10.1177/10406387251355254","DOIUrl":"10.1177/10406387251355254","url":null,"abstract":"Stray cats and dogs have been reported to shed Leptospira spp., and wild rats are speculated to be involved. We aimed to elucidate the role of wild rats in transmitting Leptospira to stray cats and dogs in Malaysia. We tested sera from 124 wild rats with the microscopic agglutination test (MAT): 88 of 122 (72%) sera were positive (titer ≥1:100), with the predominant serovars Icterohaemorrhagiae, Bataviae, Ballum, Javanica, Lai, and Pomona. With a Leptospira-specific PCR assay, we detected pathogenic Leptospira spp. in 33 of 124 (27%) kidney samples and 13 of 79 (16%) urine samples. Isolates obtained by culture of rat kidney and urine were identified to the species level with MAT using hyperimmune sera and the PCR assay. From 29 isolates, 2 pathogenic species were identified: L. interrogans serovar Bataviae and L. borgpetersenii serovar Javanica. Phylogenetic analysis using partial 16S rDNA sequences of the Leptospira spp. from the wild rats indicated that the species were similar to isolates from stray cats and dogs in previous studies. We confirmed that wild rats carried pathogenic Leptospira spp. and were a potential source of leptospiral infection of stray cats and dogs in Malaysia.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"800-805"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267210/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144642877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0