Journal of Veterinary Diagnostic Investigation最新文献_第4页

Targeted quantification of per- and polyfluoroalkyl substances (PFASs) in livestock serum by liquid chromatography-high-resolution mass spectrometry. 利用液相色谱-高分辨质谱法对家畜血清中的全氟和多氟烷基物质 (PFAS) 进行定向定量。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-08-17 DOI: 10.1177/10406387241268224

Xin Xu, Lisa A Murphy

{"title":"Targeted quantification of per- and polyfluoroalkyl substances (PFASs) in livestock serum by liquid chromatography-high-resolution mass spectrometry.","authors":"Xin Xu, Lisa A Murphy","doi":"10.1177/10406387241268224","DOIUrl":"10.1177/10406387241268224","url":null,"abstract":"Per- and polyfluoroalkyl substances (PFASs) have attracted increasing attention due to their persistence in the environment and potential adverse effects on human and animal health. The detection and quantification of PFASs in livestock could substantially contribute to monitoring their presence within the food chain. We developed a targeted quantification method for 34 PFASs in livestock serum by liquid chromatography-high-resolution mass spectrometry (LC-HRMS). We used protein precipitation for serum sample extraction and accurate mass measurement of targeted PFAS compounds for quantification. We validated the method with various analytical parameters, achieving accuracy of 70-120% and precision of <20%. The method also demonstrated good analytical sensitivity, with a limit of detection of <0.051 ng/mL and a limit of quantification of <0.175 ng/mL. When applying the developed method to actual serum samples from a variety of livestock, we successfully identified and quantified various PFASs in different livestock species. Our method has the potential to be a valuable tool for veterinary laboratory analysis of PFAS contamination in livestock.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"902-906"},"PeriodicalIF":1.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic osteoarthritis caused by Propionibacterium australiense infection in a captive sand gazelle. 圈养沙羚感染澳大利亚丙酸杆菌引起的慢性骨关节炎。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-08-05 DOI: 10.1177/10406387241263329

Emily M King, James M Wilson, Eric T Hostnik, Priya Bapodra, Randall E Junge, Andrew J Niehaus, Sushmitha S Durgam, Megan E Schreeg

{"title":"Chronic osteoarthritis caused by Propionibacterium australiense infection in a captive sand gazelle.","authors":"Emily M King, James M Wilson, Eric T Hostnik, Priya Bapodra, Randall E Junge, Andrew J Niehaus, Sushmitha S Durgam, Megan E Schreeg","doi":"10.1177/10406387241263329","DOIUrl":"10.1177/10406387241263329","url":null,"abstract":"Osteoarthritis is a common cause of morbidity and mortality in geriatric gazelles. Propionibacterium australiense has been reported as a cause of systemic granulomas in cattle, but there are no descriptions of this bacteria infecting other species nor causing osteoarthritis, to our knowledge. An 8-y-old, castrated male, sand gazelle (Gazella leptoceros leptoceros) was managed for chronic, intermittent, progressive osteoarthritis of the right tarsus. Serial biopsies revealed pyogranulomatous dermatitis with intralesional bacteria. Serial diagnostic imaging identified osseous and soft tissue proliferation with draining tracts. Treatments over 1 y included broad-spectrum antibiotics, anti-inflammatories, joint debridement, and infusion with platelet-rich plasma and stem cells. Despite therapy, lameness persisted, azotemia developed, and subsequently, the animal was euthanized. On postmortem examination, the periarticular tissue of the right tarsus was markedly expanded by pyogranulomas and fibrosis. Histologically, the synovium, joint capsule, and overlying soft tissues were markedly expanded by pyogranulomas and numerous gram-positive and acid-fast-negative filamentous bacteria surrounded by Splendore-Hoeppli material. Within the joint, there was regionally extensive cartilage ulceration, osteonecrosis, osteolysis, and pannus formation. PCR assay of affected formalin-fixed, paraffin-embedded tissue amplified segments of 16S rRNA and β subunit of bacterial RNA polymerase (rpoB) genes with 99.7% and 95.6% identity to P. australiense. This bacterium should be considered a differential for chronic pyogranulomatous osteoarthritis in gazelles.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"816-822"},"PeriodicalIF":1.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Putative diagnosis of neuromuscular and vascular hamartoma: 2 cases in dogs and review of the veterinary literature. 神经肌肉和血管火腿肠瘤的推定诊断：2例狗病例及兽医文献综述。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-08-05 DOI: 10.1177/10406387241266862

Daniel Felipe Barrantes Murillo, Tatiane Terumi Negrão Watanabe, Emily J Brinker, Bradley P Book, Laura Dvorak, Linda Huang

{"title":"Putative diagnosis of neuromuscular and vascular hamartoma: 2 cases in dogs and review of the veterinary literature.","authors":"Daniel Felipe Barrantes Murillo, Tatiane Terumi Negrão Watanabe, Emily J Brinker, Bradley P Book, Laura Dvorak, Linda Huang","doi":"10.1177/10406387241266862","DOIUrl":"10.1177/10406387241266862","url":null,"abstract":"Neuromuscular and vascular hamartoma (NMVH) is an infrequent gastrointestinal lesion described in human and veterinary medical literature. The histologic features of this entity are haphazardly arranged fascicles of smooth muscle, nerve fibers, scattered ganglion cells, and hemangiomatous blood vessels. Here we describe 2 putative cases of NMVH in a 1.7-y-old, intact female Anatolian mixed-breed dog and a 4-mo-old intact male Akita dog. Both animals had gastrointestinal clinical signs, including hematochezia, and on exploratory laparotomy, intussusception was confirmed. Histologic examination confirmed NMVH within the cecal wall in both cases using a panel of immunohistochemical (IHC) markers for vascular structures (CD31), smooth muscle (alpha-smooth muscle actin [α-SMA]), and nerves (glial fibrillary acidic protein [GFAP] and S100). The complete surgical excision of the lesion in both animals was considered curative without persistent clinical signs 14 mo and 12 mo, respectively, after surgery.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"879-885"},"PeriodicalIF":1.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipofuscin accumulates in ganglionic neurons in chronic equine dysautonomia. 脂褐素在慢性马自主神经功能障碍的神经节神经元中积聚。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-08-07 DOI: 10.1177/10406387241265715

Lydia Tan Yi Shean, Elspeth M Milne, Darren J Shaw, Scott Maxwell, Jorge Del-Pozo

{"title":"Lipofuscin accumulates in ganglionic neurons in chronic equine dysautonomia.","authors":"Lydia Tan Yi Shean, Elspeth M Milne, Darren J Shaw, Scott Maxwell, Jorge Del-Pozo","doi":"10.1177/10406387241265715","DOIUrl":"10.1177/10406387241265715","url":null,"abstract":"Lipofuscin is a complex mixture of highly oxidized, cross-linked macromolecules that accumulates in neurons with age and some neurodegenerative diseases. Equine dysautonomia (ED) is a polyneuropathy that mainly affects autonomic and enteric nervous systems, resulting in alimentary tract dysfunction. Our main aim was to determine whether neuronal lipofuscin increased with increasing duration of ED. We investigated the prevalence of lipofuscin in cranial cervical ganglia of horses with acute (AED), subacute (SED), and chronic ED (CED), young controls (of similar age to ED cases), and aged controls (n = 8 per group). We used Schmorl stain for histologic detection of lipofuscin and assessed its accumulation in neurons using image analysis software. The percentage of neurons positive for lipofuscin increased with age in individual groups and all groups combined (p < 0.001). There were fewer positive neurons in AED and SED compared to aged controls (p < 0.001) and more in CED than AED cases (p = 0.042) and young controls (p = 0.012). We found a strong positive correlation between percentage positive neurons and percentage positive area of the neuron containing lipofuscin for combined groups (p < 0.001). Although neuronal lipofuscin increased in cranial cervical ganglion in CED cases, it remains to be determined whether this is a cause or consequence of neuronal degeneration.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"864-869"},"PeriodicalIF":1.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141902184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular detection of Chlamydia abortus in endometrial biopsies of mares from western Canada. 加拿大西部母马子宫内膜活检中流产衣原体的分子检测。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-08-17 DOI: 10.1177/10406387241267864

R Madison Ricard, Bruce Wobeser

引用次数: 0

Rosette-forming multicentric B-cell lymphoma in a giant panda. 大熊猫身上的玫瑰花状多中心 B 细胞淋巴瘤。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-09-06 DOI: 10.1177/10406387241267887

Anne C N Tse, Christopher J Brackman, Carlton P M Yuen, Christopher M Perkins, Paolo Martelli, May P Y Tse

引用次数: 0

Synovial myxoma with cyst formation in the hip joint of a central bearded dragon. 中央胡须龙髋关节滑膜肌瘤伴囊肿形成。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-09-12 DOI: 10.1177/10406387241277230

Melina Rasper-Hössinger, Patricia Edith Kunze, David Schmid, Eva Dervas

{"title":"Synovial myxoma with cyst formation in the hip joint of a central bearded dragon.","authors":"Melina Rasper-Hössinger, Patricia Edith Kunze, David Schmid, Eva Dervas","doi":"10.1177/10406387241277230","DOIUrl":"10.1177/10406387241277230","url":null,"abstract":"Degenerative bone lesions are rarely described in reptiles and belong mainly to the broad spectrum of metabolic bone diseases. Here we describe a 7-y-old female central bearded dragon (Pogona vitticeps) with a complex unilateral neoplastic lesion in the hip joint. The animal was presented because of severe progressive swelling of the left hindlimb, apathy, and weight loss. The swelling was soft and surrounded the left femur. Full-body radiographs were performed in 2 orthogonal projections. The main radiologic findings were severe soft tissue swelling centered on the proximal third of the left femur and an absent left femoral head. The caretaker elected euthanasia, and a postmortem examination was performed, followed by subsequent histologic examination. The swelling consisted of variably sized myxomatous proliferations and cysts that invaded the femoral bone. Furthermore, several long bones had lesions consistent with metabolic bone and degenerative joint diseases. Synovial myxomas are rare lesions of the joints that have, to our knowledge, not been described previously in reptiles.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"891-895"},"PeriodicalIF":1.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11523163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142290177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotyping of infectious bronchitis virus in Canada. 加拿大传染性支气管炎病毒基因分型。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-08-06 DOI: 10.1177/10406387241265955

Davor Ojkic, Leonardo Susta, Emily Martin

引用次数: 0

Dermal melanoma with plasmacytoid differentiation in a dog. 一只狗患有浆细胞分化的皮肤黑色素瘤。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-08-20 DOI: 10.1177/10406387241273907

Ricardo E Mendes, Ronald H Tolbert, Catherine E Thorn, Daniel R Rissi

{"title":"Dermal melanoma with plasmacytoid differentiation in a dog.","authors":"Ricardo E Mendes, Ronald H Tolbert, Catherine E Thorn, Daniel R Rissi","doi":"10.1177/10406387241273907","DOIUrl":"10.1177/10406387241273907","url":null,"abstract":"A 10-y-old spayed female Staffordshire Terrier dog was evaluated because of a cutaneous left ear base mass. Cytology revealed sheets of cells with anisocytosis and anisokaryosis, round-to-oval or plasmacytoid cytoplasm, and round, central, or eccentric nuclei; binucleate cells were present. Cytologic findings were consistent with a round cell tumor (plasmacytoma or agranular mast cell tumor), amelanotic melanoma, or anaplastic carcinoma. Histologically, neoplastic cells were polygonal to elongate, with round-to-oval nuclei and prominent nucleoli and arranged in sheets and nests on a fibrovascular stroma. Neoplastic cells with plasmacytoid morphology (round, glassy, eosinophilic cytoplasm with eccentric nuclei) were present in ~30% of the neoplasm. There were 18 mitoses in 2.37 mm2 (10 FN22/40× fields). Neoplastic cells had cytoplasmic immunolabeling for melan A and PNL2 and no immunolabeling for AE3/1 and MUM1, consistent with a dermal melanoma with plasmacytoid differentiation. The patient was re-evaluated ~1 mo after the first biopsy because of local recurrence of the original mass and new masses on the interscapular area and right elbow; these neoplasms were histologically identical to the original submission, plus scattered neoplastic cells in the new masses contained brown cytoplasmic pigment. The dog was euthanized because of swelling and hemorrhage of the tumors and right pelvic limb lameness. Our findings were consistent with a dermal melanoma with plasmacytoid features that were similar to human plasmacytoid melanoma, a rare variant of human melanoma that is diagnostically challenging as it may mimic a plasmacytoma.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"941-944"},"PeriodicalIF":1.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529057/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142008987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MRI changes observed in a case of atypical scrapie in a 7-year-old Herdwick ewe. 在一例 7 岁牧羊母羊非典型瘙痒症病例中观察到的核磁共振成像变化。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-11-01 Epub Date: 2024-09-06 DOI: 10.1177/10406387241267849

Sander Prins, Kim Hamer, Ana Cloquell, John Spiropoulos, Neil Sargison, Piet Vellema

{"title":"MRI changes observed in a case of atypical scrapie in a 7-year-old Herdwick ewe.","authors":"Sander Prins, Kim Hamer, Ana Cloquell, John Spiropoulos, Neil Sargison, Piet Vellema","doi":"10.1177/10406387241267849","DOIUrl":"10.1177/10406387241267849","url":null,"abstract":"Atypical scrapie is a transmissible spongiform encephalopathy that is rarely diagnosed in living animals. In March 2022, a 7-y-old Herdwick ewe was referred to the Scottish Centre for Production Animal Health and Food Safety because of circling behavior and ill thrift. The ewe had a low body condition score, was obtunded, with a wide-based stance of the pelvic limbs, and was circling to the left. Hematologic, biochemical, and CSF analyses were unremarkable, but postmortem magnetic resonance imaging (MRI) findings were consistent with diffuse, bilateral, and symmetrical atrophy of the forebrain and ventriculomegaly. The clinical signs, the involvement of an individual older ewe, and the MRI results led to the clinical diagnosis of scrapie. Immunohistochemistry on the fixed brain, performed by the U.K. Animal and Plant Health Agency, revealed deposits of PrPSc, which is a specific disease marker of transmissible spongiform encephalopathies, mainly in the cerebellum and at lower concentrations in the cerebrum and obex, consistent with the diagnosis of atypical scrapie. MRI findings in a sheep with atypical scrapie have not been described previously, to our knowledge. Scrapie should be included in the list of clinical differential diagnoses when veterinarians are presented with sheep with progressive neurologic signs of several weeks' duration.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"842-846"},"PeriodicalIF":1.2,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11512460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0