Journal of Veterinary Diagnostic Investigation最新文献_第4页

Corrigendum to: Oxytetracycline-resistant Paenibacillus larvae identified in commercial beekeeping operations in Saskatchewan using pooled honey sampling. 勘误表：在萨斯喀彻温省的商业养蜂业中使用混合蜂蜜取样鉴定出具有土霉素抗性的芽孢杆菌幼虫。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-10 DOI: 10.1177/10406387241308319

引用次数: 0

Suspected fenugreek (Trigonella foenum-graecum L.) toxicosis in a herd of Saskatchewan beef cattle. 萨斯喀彻温省一群肉牛疑似葫芦巴（葫芦巴）中毒。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-09 DOI: 10.1177/10406387241307974

Vanessa E Cowan, Roman V Koziy, Laurie Zemlak, John R Campbell

{"title":"Suspected fenugreek (Trigonella foenum-graecum L.) toxicosis in a herd of Saskatchewan beef cattle.","authors":"Vanessa E Cowan, Roman V Koziy, Laurie Zemlak, John R Campbell","doi":"10.1177/10406387241307974","DOIUrl":"10.1177/10406387241307974","url":null,"abstract":"An apparent outbreak of fenugreek forage toxicosis occurred in a beef cattle herd near Moose Jaw, Saskatchewan in February-May 2022. The herd had consumed fenugreek hay from late fall to early winter. Clinical signs included various degrees of weakness, ataxia, knuckling, walking on hocks, and recumbency. All adult cattle in the herd eventually died or were euthanized. Feed analysis did not reveal nutritional deficiencies or mycotoxin contamination. Liver mineral and vitamin status of affected animals did not indicate any consistent abnormality. The last live cow in the herd was presented to a veterinary teaching hospital for evaluation and subsequent postmortem examination. Major postmortem findings included emaciation, and sciatic nerve and spinal cord axonal degeneration. Histologic examination of the sciatic nerve showed Wallerian-like axonal degeneration, increased Schwann cell nuclei, and endoneurial fibrosis. Histologic examination of the spinal cord showed infrequent myelin sheath dilation and digestion chambers within white matter. These results are consistent with other reports of natural and experimental outbreaks of fenugreek poisoning in livestock. To our knowledge, fenugreek toxicosis has not been reported previously in Canada. We conclude that caution should be taken when feeding fenugreek hay to cattle.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"329-333"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11719421/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute outbreak of polioencephalomalacia in adult beef cows on pasture associated with feeding broiler litter.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-02-02 DOI: 10.1177/10406387251315724

Savannah R Charnas, Timothy Walsh, Brandi Tharp, Scott A Fritz

{"title":"Acute outbreak of polioencephalomalacia in adult beef cows on pasture associated with feeding broiler litter.","authors":"Savannah R Charnas, Timothy Walsh, Brandi Tharp, Scott A Fritz","doi":"10.1177/10406387251315724","DOIUrl":"10.1177/10406387251315724","url":null,"abstract":"Polioencephalomalacia (PEM) is a neurologic disorder of ruminants that can affect cattle as a result of high levels of sulfur in the diet. Sulfur can be ingested by an animal through feed or water, and the mineral status of the animal can affect how much sulfur is ultimately absorbed. Broiler (poultry) litter is sometimes used in cattle diets as an economical protein source, although it runs the risk of supplying too much sulfur to the bovine diet. Here we report a case in which 15 cows had neurologic signs before death. Initial testing ruled out other causes of acute death, and histologic findings confirmed a diagnosis of PEM. The sulfur concentration was ~0.70% of the total mixed ration (TMR), which is above the maximum tolerable dietary concentration of sulfur. The broiler litter contributed nearly all of the sulfur to this ration and was the cause of the PEM experienced in this herd. When feeding by- and co-products as part of a TMR, it is important for producers to analyze the feed to mitigate risk and ensure good health.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"385-388"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11789040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using HALO digital image analysis for automated detection of bovine viral diarrhea virus antigen in ear-notch specimens. 利用HALO数字图像分析技术自动检测牛病毒性腹泻病毒抗原耳缺口标本。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1177/10406387241307643

Susanne Je-Han Lin, Drew R Magstadt, Rachel J Derscheid, Eric R Burrough

{"title":"Using HALO digital image analysis for automated detection of bovine viral diarrhea virus antigen in ear-notch specimens.","authors":"Susanne Je-Han Lin, Drew R Magstadt, Rachel J Derscheid, Eric R Burrough","doi":"10.1177/10406387241307643","DOIUrl":"10.1177/10406387241307643","url":null,"abstract":"Detecting calves that are persistently infected with bovine viral diarrhea virus (BVDV) is essential to disease prevention. Immunohistochemistry (IHC) performed on formalin-fixed, paraffin-embedded ear-notch samples is commonly used for surveillance detection of BVDV antigens. However, due to the low percentage of positive samples in most submissions, the current workflow often entails considerable time reviewing negative results. Herein we aimed to utilize digital pathology and whole-slide imaging, coupled with advanced image analysis software, to enhance the efficiency of positive IHC detection in surveillance. Despite some challenges encountered during the implementation phase, the benefits of the reduced potential for human error and significant time savings for technicians and pathologists are evident. The screening of 518 slides, containing 2,884 ear notches, reached 97.4% sensitivity and 89.4% specificity compared to the gold standard of direct human assessment. The time taken for the personnel to operate the software and organize results was significantly shorter than the time needed for technicians and pathologists to manually examine the slides. Future refinements in software integration, staining protocols, and QC measures promise to further optimize this approach.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"354-357"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707765/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disseminated nontuberculous mycobacteriosis due to Mycobacterium avium subsp. hominissuis infection in a reindeer from a zoo.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-28 DOI: 10.1177/10406387241307644

John M Winter, Jennifer N Langan, Jennifer A Landolfi, Tyler Thacker, Katie W Delk

{"title":"Disseminated nontuberculous mycobacteriosis due to Mycobacterium avium subsp. hominissuis infection in a reindeer from a zoo.","authors":"John M Winter, Jennifer N Langan, Jennifer A Landolfi, Tyler Thacker, Katie W Delk","doi":"10.1177/10406387241307644","DOIUrl":"10.1177/10406387241307644","url":null,"abstract":"Bacteria in the Mycobacterium tuberculosis complex and nontuberculous mycobacteria may affect a variety of animal species under human care and pose public health risks as zoonotic pathogens. A case of sudden onset of lethargy and increased respiratory effort in a 5-y-old, intact female reindeer (Rangifer tarandus) under managed care had progressed to severe dyspnea despite aggressive treatment. The animal was euthanized due to poor prognosis. Postmortem findings included: disseminated miliary nodules in the lungs, pleura, small intestine, liver, and spleen; enlarged mesenteric lymph nodes; dilated mesenteric and serosal lymphatic vessels; and renal infarcts. Histologically, granulomatous lymphadenitis and lymphangitis with intrahistiocytic acid-fast bacilli were observed. Mycobacterium sp. DNA was detected in lung via real-time PCR. Mycobacterial culture and sequencing identified Mycobacterium avium subsp. hominissuis (MAH) within pulmonary lesions. Infection with MAH has been reported in humans and many animal species; this nontuberculous mycobacterial infection may be an emerging concern in animals under managed care. To our knowledge, MAH infection has not been reported previously in reindeer.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"324-328"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11775928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Frequency and antimicrobial susceptibility of Staphylococcus aureus isolated from clinical bovine mastitis cases in British Columbia, Canada. 加拿大不列颠哥伦比亚省临床牛乳腺炎病例分离的金黄色葡萄球菌的频率和抗菌药物敏感性。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2024-12-26 DOI: 10.1177/10406387241306096

Melissa Leon, Joseph Rubin, Stephen Raverty, Kazal Ghosh

{"title":"Frequency and antimicrobial susceptibility of Staphylococcus aureus isolated from clinical bovine mastitis cases in British Columbia, Canada.","authors":"Melissa Leon, Joseph Rubin, Stephen Raverty, Kazal Ghosh","doi":"10.1177/10406387241306096","DOIUrl":"10.1177/10406387241306096","url":null,"abstract":"Staphylococcus aureus is one of the most important bacteria responsible for clinical bovine mastitis globally, leading to significant economic losses in the dairy industry. Antimicrobials used to treat and prevent mastitis can lead to antimicrobial resistance (AMR) in S. aureus. We retrospectively evaluated AMR of S. aureus isolates from clinical bovine mastitis cases submitted to the Animal Health Centre in British Columbia from 2013 to May 2024. S. aureus was isolated from 15.0% of submitted bovine milk samples. Antimicrobial susceptibility testing was done on 611 of 1,347 S. aureus isolates. No methicillin-resistant S. aureus isolates were detected based on cefoxitin susceptibility test results, which is encouraging. The highest frequencies of resistance were found for penicillin (46.6%) and ampicillin (42.1%). The lowest frequencies of resistance were to ceftiofur and sulfamethoxazole-trimethoprim (0.2% each) and cephalothin (0.3%). AMR trends over our study period were generally stable, except for penicillin and ampicillin; penicillin resistance increased from 15.2% to 71.1%, and ampicillin resistance increased from 18.2% to 70.1%. The information in our study could help guide clinicians when choosing antimicrobial treatments to treat mastitis caused by S. aureus, particularly in the province of British Columbia. Because S. aureus has a broad host range and is of importance to both human and veterinary medicine, continued monitoring to detect the emergence of resistance is warranted.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"371-374"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11672364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142895782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ovarian neuroglial choristoma in a dog.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-02-02 DOI: 10.1177/10406387251316831

Na-Young Lee, Jeong-Seop Oh, Dae-Yong Kim, Tae-Kyun Im, Dae Young Kim

引用次数: 0

Detection of bovine respiratory disease complex-related pathogens in nasopharynx-associated lymphoid tissue.

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-02-17 DOI: 10.1177/10406387251318415

Asmaa A Hegazy, Mutsumi Nakai, Naoyuki Fuke, Amaal E Hussein, Hiroaki Kondo, Takuya Hirai

{"title":"Detection of bovine respiratory disease complex-related pathogens in nasopharynx-associated lymphoid tissue.","authors":"Asmaa A Hegazy, Mutsumi Nakai, Naoyuki Fuke, Amaal E Hussein, Hiroaki Kondo, Takuya Hirai","doi":"10.1177/10406387251318415","DOIUrl":"10.1177/10406387251318415","url":null,"abstract":"Bovine respiratory disease complex (BRDC) is a multi-etiologic disease that significantly impacts the cattle industry. Nasopharynx-associated lymphoid tissue (NALT) is the nasal mucosal immune system that protects against various pathogens; however, reports on its pathology are limited. We compared the pathologic impact of BRDC-related viruses on the NALT and lungs of 10 pneumonic and 3 negative control Japanese black (JB) calves. Three bovine viral diarrhea virus (BVDV) persistently infected Holstein calves were examined as positive control; all 3 calves had BVDV in situ hybridization (ISH)-positive signals in NALT follicular macrophages and lymphocytes, suggesting viral replication in these cells. NALT and lungs of pneumonic JB calves had weak-positive BVDV results in PCR and negative results in ISH, indicating late-stage transient BVDV infection. The finding of BVDV in unvaccinated pneumonic JB calves confirmed the involvement of a BVDV field strain. PCR detection of bovine coronavirus (BCoV) and bovine respiratory syncytial virus (BRSV) in NALT along with ISH BCoV-positive signals in NALT epithelial cells confirms infection by those viruses. Pneumonic JB calves had nasopharyngitis and pneumonia, with the same bacteria present in nasopharyngeal swabs and lungs, indicating that, in these animals, the bacteria proliferating in the nasopharynx probably migrated to the lungs via inhalation. BVDV transient infection in the NALT may induce local immunosuppression; BCoV and BRSV infections are thought to damage epithelial cells, facilitating bacterial infection of the NALT and lungs. Our results confirm that bovine NALT is a location for viral replication and may be associated with BRDC development in cattle.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"284-297"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11833804/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streptococcus hillyeri isolated from septic pleuritis in a horse. 从马脓毒性胸膜炎中分离出希利氏链球菌。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1177/10406387241306724

Ervin Albert, Imre Biksi, Levente Laczkó, László Miló, Karolina Cseri, Renáta Bőkényné Tóth, Dalma Papp, Dóra Halmay, Emese Bódai, Zoltán Bakos

{"title":"Streptococcus hillyeri isolated from septic pleuritis in a horse.","authors":"Ervin Albert, Imre Biksi, Levente Laczkó, László Miló, Karolina Cseri, Renáta Bőkényné Tóth, Dalma Papp, Dóra Halmay, Emese Bódai, Zoltán Bakos","doi":"10.1177/10406387241306724","DOIUrl":"10.1177/10406387241306724","url":null,"abstract":"Here we report the isolation of Streptococcus hillyeri from a thoracic sample from a horse. A 17-y-old Hungarian Sport Horse mare was referred to the equine clinic of the University of Veterinary Medicine Budapest, Hungary, with suspected pleuritis. Upon arrival, the horse was febrile and had tachycardia, severe inspiratory dyspnea, and tachypnea. Thoracic ultrasonography revealed severe bilateral pleural effusion, and a large area of lung consolidation. After sampling of both hemithoraces, 66 L of turbid exudate were drained. Based on these findings, a tentative diagnosis of septic pleuritis was made, and the horse was immediately started on a course of broad-spectrum antibiotics, a NSAID, an anticoagulant, and intravenous fluids. Despite intensive care, the clinical parameters deteriorated, and the horse was euthanized 6 d later. Cytology confirmed septic pleuritis, with short chains or groups of coccoid bacteria. Anaerobic culture yielded gram-positive cocci from both hemithoraces in almost pure culture, which we identified as S. hillyeri by 16S rDNA and whole-genome analysis. Additionally, we identified 4 previously unassigned Streptococcus sp. sequences as S. hillyeri. Of these, 3 were obtained from aborted equine fetuses and a fourth from a donkey mastitis case, supporting the pathogenic nature of S. hillyeri in these host species.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"393-397"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peliosis hepatis and hepatic fibrosis in a dog infected with multiple Bartonella species. 犬感染多种巴尔通体并发肝纤维化。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-03-01 Epub Date: 2025-01-07 DOI: 10.1177/10406387241310868

Cynthia Robveille, Cody Atkinson, Jerry Cowart, Ricardo G Maggi, Neelesh Narurkar, Edward B Breitschwerdt

{"title":"Peliosis hepatis and hepatic fibrosis in a dog infected with multiple Bartonella species.","authors":"Cynthia Robveille, Cody Atkinson, Jerry Cowart, Ricardo G Maggi, Neelesh Narurkar, Edward B Breitschwerdt","doi":"10.1177/10406387241310868","DOIUrl":"10.1177/10406387241310868","url":null,"abstract":"A 13-y-old, spayed female dog had regenerative anemia, lymphopenia, hypoalbuminemia, and elevated hepatic biochemical parameters. Liver biopsy revealed hepatic peliosis (hepatic sinusoidal angiectasis), frequently associated with perisinusoidal fibrosis. The dog was seroreactive to Bartonella antigens by indirect fluorescent antibody assays, and quantitative PCR from blood identified Bartonella vinsonii subsp. berkhoffii genotype II. The dog was euthanized 9 mo later because of acute decompensation. Autopsy revealed icteric adipose tissues, end-stage liver, and abdominal effusion. Microscopically, there was marked mixed-cell chronic hepatitis with hepatocellular loss, nodular hepatocellular regeneration, and capillary proliferation. Retrospective molecular testing documented B. koehlerae and B. rochalimae DNA in the dog's blood at 2 or more times during liver disease progression. B. koehlerae DNA was also amplified and sequenced from the autopsy sample of liver. Our case emphasizes that Bartonella infection may be associated with hepatic peliosis and end-stage liver in dogs and expands the spectrum of Bartonella species that potentially play a role in canine hepatic diseases.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"358-362"},"PeriodicalIF":1.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142950680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0