Journal of Veterinary Diagnostic Investigation最新文献_第4页

Evaluation of sampling and laboratory parameters contributing to successful isolation of senecavirus A1 from pigs. 对成功分离猪塞尼卡病毒A1的采样和实验室参数进行评估。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-06-23 DOI: 10.1177/10406387251327604

BoMyoung Kim, Munashe Chigerwe, Beate M Crossley

{"title":"Evaluation of sampling and laboratory parameters contributing to successful isolation of senecavirus A1 from pigs.","authors":"BoMyoung Kim, Munashe Chigerwe, Beate M Crossley","doi":"10.1177/10406387251327604","DOIUrl":"10.1177/10406387251327604","url":null,"abstract":"Senecavirus A1 (SVA; Picornaviridae, Senecavirus valles) infection causes an emerging infectious disease of swine that often leads to cutaneous vesicles on the snout and coronary bands and in the oral cavity of affected pigs, and causes epidemic transient neonatal loss in piglets. Virus isolation (VI) of SVA is required for viability testing, reference stock production, and monitoring virus evolution. We evaluated sampling and testing parameters leading to the successful propagation of SVA with a cross-sectional design based on 1,102 samples submitted to a reference laboratory. Logistic regression was used to evaluate the association between 16 variables and the outcome. When April was considered the reference month, the odds of SVA isolation failure were 17 and 100 times more likely during July and August, respectively. When foot samples were considered the referent, the odds of VI were 1.7 times more likely in unspecified samples. Compared to pooled samples, the odds of successful SVA isolation in individual samples were 8 and 45 times more likely in July and August, respectively. Laboratory parameters were not significantly associated with SVA isolation. The logistic model was moderately accurate (AUC = 0.68; 95% CI: 0.65-0.72%), suggesting that the model should be used cautiously for practical purposes. High environmental temperatures might negatively affect virus recovery. Using individual samples might improve SVA recovery when high ambient temperatures are recorded, as in the months of July and August.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"759-763"},"PeriodicalIF":1.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic approach to swinepox virus infection in a German 2-site swine production unit. 德国2点养猪场猪痘病毒感染的诊断方法

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-08-30 DOI: 10.1177/10406387251366960

Susanne Richter, Friedrich Schmoll, Daniel Polzer, Christoph Leth, Sandra Revilla-Fernández, Lukas Schwarz, Angelika Auer, Tatjana Sattler

{"title":"Diagnostic approach to swinepox virus infection in a German 2-site swine production unit.","authors":"Susanne Richter, Friedrich Schmoll, Daniel Polzer, Christoph Leth, Sandra Revilla-Fernández, Lukas Schwarz, Angelika Auer, Tatjana Sattler","doi":"10.1177/10406387251366960","DOIUrl":"https://doi.org/10.1177/10406387251366960","url":null,"abstract":"In 2008, nearly 50% of weaned piglets at a German 2-site production unit in Saxony-Anhalt had skin lesions 1-2 wk after relocation into the nursery. First clinical signs were maculae, followed by papules, pustules, and finally crusts, distributed over the dorsal and lateral body flank. Tentative clinical diagnosis was an infection with swinepox virus (SWPV; family Poxviridae, taxon species Suipoxvirus swinepox). Electron microscopy confirmed within one hour that the causal agent was a brick-shaped poxvirus, and routine PCR validated the poxvirus detection; PCR for Orthopoxvirus was negative. Phylogenetic analysis of the thymidine kinase genes from different poxviruses and from our SWPV isolates, 3 isolates from Germany, and 1 isolate from Austria, provided a good picture of evolutionary relationships of poxvirus genera, which was also consistent with phylogenetic analysis of poxviruses based on other genes. The German and Austrian isolates from domestic pigs were 99.8-100% identical to previously isolated German SWPV from wild boar and domestic pigs. All isolates belonged to the North American/European lineage. In a second step, SWPV assembly in naturally infected domestic pigs was analyzed by ultrathin sectioning. The virus assembly resembled that of other poxviruses and completed gaps in the SWPV morphogenesis model described in prior publications. Because there is no specific therapy, recommended interventions were improvements in biosecurity measures, especially hygiene management and disinfection procedures at the farm and within the transporters between the farrowing unit and the nursery. No further infections with SWPV were seen 5-6 wk after commencement of the hygiene interventions.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251366960"},"PeriodicalIF":1.1,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12398465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Giant cell hepatitis with Platynosomum illiciens infection in a Japanese domestic cat. 日本家猫巨细胞肝炎伴白颈虫感染。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-08-29 DOI: 10.1177/10406387251365607

Natsumi Kobayashi, Aya Masuda, Jun Matsumoto, Natsumi Asai, Nobuhiko Hida, Tomoaki Murakami

引用次数: 0

Comparison of human point-of-care glucometer (Guide Me) and lactometer (Lactate Plus) to an automated chemistry analyzer for measurement of blood concentrations of lactate and glucose in juvenile commercial pigs. 将人用即时血糖仪（Guide Me）和乳酸计（Lactate Plus）与自动化学分析仪进行比较，以测量幼年商品猪血液中乳酸和葡萄糖的浓度。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-08-29 DOI: 10.1177/10406387251366277

Chiara E Hampton, Stephanie A Kleine, Lucille R Vanecek, Christopher K Smith, Gregory A Shanks, Cary Springer, Bente Flatland, Luca Giori

{"title":"Comparison of human point-of-care glucometer (Guide Me) and lactometer (Lactate Plus) to an automated chemistry analyzer for measurement of blood concentrations of lactate and glucose in juvenile commercial pigs.","authors":"Chiara E Hampton, Stephanie A Kleine, Lucille R Vanecek, Christopher K Smith, Gregory A Shanks, Cary Springer, Bente Flatland, Luca Giori","doi":"10.1177/10406387251366277","DOIUrl":"https://doi.org/10.1177/10406387251366277","url":null,"abstract":"Porcine glucose and lactate concentrations measured with a point-of-care glucometer (POC-Glu; Guide Me) and a POC lactometer (POC-Lac; Lactate Plus) were compared to those from a comparative method (CM; COBAS-501c). Fresh whole blood samples (n = 175 glucose; n = 272 lactate) from 10 healthy juvenile commercial-cross pigs (5 barrows, 5 gilts; 79-91-d-old) were collected over 12 d under various conditions. Comparisons were made with the Passing-Bablok regression and Bland-Altman method with multiple measurements per subject. The allowable total error (TEa) was set at 20% for glucose and 40% for lactate. Correlation with CM was high for POC-Glu (r = 0.886; 95% CI [0.849, 0.914]) and very high for POC-Lac (r = 0.935; 95% CI [0.918, 0.949]) with constant and proportional biases (intercept = -0.52, 95% CI [-0.98, -0.13] mmol/L, slope = 0.06, 95% CI [0.06, 0.07] mmol/L for glucose; intercept = 0.075, 95% CI [0.050, 0.104] mmol/L, slope = 0.797, 95% CI [0.775, 0.819] mmol/L for lactate). Acceptance limits based on combined inherent imprecision (CIP) were ±5.2% for glucose and ±8.1% for lactate. The observed total errors (TEobs) were 5.42% for POC-Glu and 29.9% for POC-Lac at the decision threshold of 3.0 mmol/L, both within the set TEa. Both POC-Glu and POC-Lac are practical and reliable tools for use in juvenile pigs, with satisfactory agreement to the reference method, although interpretation of POC-Lac results requires greater caution due to a significant matrix effect, underscoring the need for method- and matrix-specific RIs, particularly for lactate.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251366277"},"PeriodicalIF":1.1,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12397091/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eurytrema sp. as a cause of chronic interstitial pancreatitis in cattle in southeastern Brazil. 巴西东南部牛慢性间质性胰腺炎的起因。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-08-25 DOI: 10.1177/10406387251345888

Guilherme S Lemos, Rodrigo L A Palhano, Hudson A Santos, João P Matiello, Bárbara A Alves, Gabriela Anteveli, Tiago F Moreira, Rodrigo M Meneses, Antônio U Carvalho, Elias J Facury Filho

{"title":"Eurytrema sp. as a cause of chronic interstitial pancreatitis in cattle in southeastern Brazil.","authors":"Guilherme S Lemos, Rodrigo L A Palhano, Hudson A Santos, João P Matiello, Bárbara A Alves, Gabriela Anteveli, Tiago F Moreira, Rodrigo M Meneses, Antônio U Carvalho, Elias J Facury Filho","doi":"10.1177/10406387251345888","DOIUrl":"https://doi.org/10.1177/10406387251345888","url":null,"abstract":"We report here 6 cases of bovine eurytrematosis on 2 farms (dairy and beef cattle) in southeastern Brazil. The cattle had different primary lesions that explained their clinical conditions; however, upon autopsy, common to all animals were abnormalities in the pancreas and the presence of Eurytrema spp. All parasitized pancreases were swollen, firm, and tan to yellow-pink. Upon dissection, numerous parasites were observed, the pancreatic ducts were thickened and dilated, and the parenchyma was fibrotic. Histopathologic findings were multifocal-to-diffuse chronic interstitial pancreatitis. Despite clinical signs, such as low body condition score, compatible with eurytrematosis, it was challenging to determine whether these signs were caused primarily by the pancreatic lesion or by the primary disease affecting the cows. Bovine eurytrematosis may be associated with other underlying conditions, making its diagnosis difficult and potentially impacting the prognosis of the affected animals. Our findings underscore the harmful nature of Eurytrema coelomaticum as a parasite and emphasize that it may be more than an incidental finding at autopsy.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251345888"},"PeriodicalIF":1.1,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12378455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A divergent anatomic distribution of bovine lymphoma: time for an update. 牛淋巴瘤的发散性解剖分布：是时候更新了。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-08-25 DOI: 10.1177/10406387251366329

Ricardo E Mendes, Fernanda F Perosa, Marcia R S Ilha, Érica Boldori, Raphael V A Tonin, Erin A Graham, Manoela M Piva, Anderson H Gris, Daniel R Rissi

引用次数: 0

Estimation of minimum centrifugation time of microhematocrit tubes to obtain accurate results of packed cell volume and total solids in donkeys, dogs, sheep, and cows. 估计微红细胞压积管的最小离心时间，以获得驴，狗，羊和牛的填充细胞体积和总固体的准确结果。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-08-25 DOI: 10.1177/10406387251362461

Dreyton Oliva, Hélène Lardé, Juliette Bouillon, Ronan Whiston, Andrea Peda, Patricia Dowling, Ronan J J Chapuis

{"title":"Estimation of minimum centrifugation time of microhematocrit tubes to obtain accurate results of packed cell volume and total solids in donkeys, dogs, sheep, and cows.","authors":"Dreyton Oliva, Hélène Lardé, Juliette Bouillon, Ronan Whiston, Andrea Peda, Patricia Dowling, Ronan J J Chapuis","doi":"10.1177/10406387251362461","DOIUrl":"https://doi.org/10.1177/10406387251362461","url":null,"abstract":"To date, the minimum centrifugation times of microhematocrit tubes of blood to generate accurate PCV and total solids (TS) results have not been validated in veterinary medicine. We collected blood samples from 44 donkeys, 43 dogs, 61 sheep, and 40 cattle. We centrifuged microhematocrit tubes for 1, 2, 3, 5, and 15 min in donkeys and dogs, and 1.5, 3, 5, 10, and 15 min in ruminants. We evaluated the agreements between PCV and TS values at each time of centrifugation with the reference values at 15 min using intra-class coefficients of correlation and linear regressions. Finally, we considered the symmetrical distribution of differences between results obtained at each time of centrifugation and the reference values. We found that microhematocrit tubes centrifuged for a minimum of 3 min in donkeys and dogs, 10 min in sheep, and 5 min in cattle gave PCV and TS results in agreement with the values obtained after 15 min of centrifugation. The centrifugation time for cattle was shorter than currently advised. However, because PCV values of all cattle and most donkeys enrolled were within RIs and because no polycythemic animals were included, validation of these times may be warranted.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251362461"},"PeriodicalIF":1.1,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12378263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postmortem diagnosis of venous air embolism in a Wielkopolski horse. 威科波夫斯基马静脉空气栓塞的死后诊断。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-08-21 DOI: 10.1177/10406387251362462

Anna M Majcher, Antonia Troillet, Kerstin Gerlach, Paul R Tönnies, Walter Brehm, Reiner Ulrich

{"title":"Postmortem diagnosis of venous air embolism in a Wielkopolski horse.","authors":"Anna M Majcher, Antonia Troillet, Kerstin Gerlach, Paul R Tönnies, Walter Brehm, Reiner Ulrich","doi":"10.1177/10406387251362462","DOIUrl":"https://doi.org/10.1177/10406387251362462","url":null,"abstract":"Nine days after a surgical intervention due to an injury of the left hind hoof capsule, a 9-y-old, 538-kg female Wielkopolski horse was found recumbent in its stall with an unplugged permanent intravenous catheter positioned in the left jugular vein. Despite immediate resuscitation attempts, the animal died within minutes. Suspecting venous air embolism, radiographic imaging and detailed postmortem examinations were performed. However, visualization of the heart by radiography was hindered by the animal's body mass and postmortem changes. The autopsy followed a modified Richter method, originally developed for diagnosing air embolism in humans. The horse was positioned in left lateral recumbency to allow in situ access to the atria and venous sinuses. Gas bubbles observed in the right atrium and ventricle confirmed venous air embolism as the cause of death. Venous air embolism is a rarely documented cause of death in veterinary pathology. We present here an autopsy protocol for diagnosis of venous air embolism in large animals, which is primarily a macroscopic diagnosis.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251362462"},"PeriodicalIF":1.1,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12373652/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Relationships of heart scores and postmortem cardiac measurements in congestive heart disease in feedlot cattle. 放牧牛充血性心脏病的心脏评分与死后心脏测量的关系。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-08-16 DOI: 10.1177/10406387251357233

Katie Long, Brad J White, Eduarda Mazzardo Bortoluzzi, Luis Felipe Feitoza, Laura Carpenter, Paige Schmidt, Maddie Mancke, Rachel Champagne, Makenna Jensen, Randall Raymond, Justin Buchanan, Robert Larson, Brian Lubbers

{"title":"Relationships of heart scores and postmortem cardiac measurements in congestive heart disease in feedlot cattle.","authors":"Katie Long, Brad J White, Eduarda Mazzardo Bortoluzzi, Luis Felipe Feitoza, Laura Carpenter, Paige Schmidt, Maddie Mancke, Rachel Champagne, Makenna Jensen, Randall Raymond, Justin Buchanan, Robert Larson, Brian Lubbers","doi":"10.1177/10406387251357233","DOIUrl":"10.1177/10406387251357233","url":null,"abstract":"Congestive heart failure (CHF) in feedyard cattle is of increasing concern among producers and can be difficult to diagnose definitively postmortem. In a cross-sectional observational study, we evaluated gross pathology findings, various heart measurements, and subjective heart scores (1-5 scale: 1 = normal, 5 = severely remodeled) to identify heart disease postmortem. In postmortem examinations of 346 feedyard deaths, we classified 106 (30.6%) cases as cardiac enlargement or misshapen ventricle (CEMV) when there was an abnormal heart shape or dilated ventricle(s), and no signs of infectious heart disease. CHF was defined as a CEMV case with chronic passive congestion of the liver (i.e., nutmeg liver) and ≥2 of the following lesions: serous or serosanguineous pleural, pericardial, or peritoneal effusion. Eleven of the 346 autopsied cattle were classified as having CHF. Descriptive statistics and multivariate models were used to identify statistical associations between objective heart measurements or subjective heart scores and the prevalence of CEMV or CHF. CEMV cases had significantly increased heart widths, thinner left ventricular free walls, and expanded right ventricular lumen areas (p <0.05). The CHF model did not converge because we had too few cases to be able to evaluate associations between CHF and variables of interest.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251357233"},"PeriodicalIF":1.1,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and validation of a real-time PCR assay for Cochlosoma anatis in turkeys. 火鸡褐藻病实时PCR检测方法的建立与验证。

IF 1.1 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-08-16 DOI: 10.1177/10406387251364260

Rebecca P Wilkes, Angie Chan, Nelly Elshafie, Kabel Robbins

{"title":"Development and validation of a real-time PCR assay for Cochlosoma anatis in turkeys.","authors":"Rebecca P Wilkes, Angie Chan, Nelly Elshafie, Kabel Robbins","doi":"10.1177/10406387251364260","DOIUrl":"10.1177/10406387251364260","url":null,"abstract":"Cochlosoma anatis is a flagellated protozoan parasite in the Trichomonadidae family that causes an enteric disease of turkeys and ducks known as cochlosomosis. Following the withdrawal of effective antiprotozoal medications from commercial poultry production, this organism has become of increased concern for the turkey industry. Historically, this organism has been detected by light microscopic evaluation of enteric mucosal scrapings. However, live trophozoites must be present in the sample for this method of detection because the parasite is differentiated from other protozoa by its characteristic movement. There is currently no real-time PCR (rtPCR) assay published for this organism; thus, we designed and validated a rtPCR assay. Ten-fold serial dilutions of gBlocks DNA representing the targeted region of the organism were evaluated to determine the limit of detection of the organism, which was ~50 copies per reaction. The assay was specific for C. anatis, with no cross-detection of 3 other poultry flagellates: Histomonas meleagridis, Tetratrichomonas gallinarum, and Trichomonas gallinae. Sensitivity and specificity compared to microscopic detection of live organisms were 100%. Coinfections of C. anatis with other pathogens have been reported. Thus, this assay can be added to syndromic panels for detection of enteric pathogens of turkeys.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251364260"},"PeriodicalIF":1.1,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12357830/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144859347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0