Journal of Veterinary Diagnostic Investigation最新文献_第4页

Characterization of amyloid deposits in canine mammary neoplasms. 犬乳腺肿瘤中淀粉样蛋白沉积的特征。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-01 DOI: 10.1177/10406387251328323

Yuka Kato, Natsumi Kobayashi, Susumu Iwaide, Shumpei Hisamoto, Satoshi Koyama, Yoshiyuki Itoh, Miki Hisada, Noboru Machida, Kohji Nomura, Tomoaki Murakami

{"title":"Characterization of amyloid deposits in canine mammary neoplasms.","authors":"Yuka Kato, Natsumi Kobayashi, Susumu Iwaide, Shumpei Hisamoto, Satoshi Koyama, Yoshiyuki Itoh, Miki Hisada, Noboru Machida, Kohji Nomura, Tomoaki Murakami","doi":"10.1177/10406387251328323","DOIUrl":"10.1177/10406387251328323","url":null,"abstract":"Mammary tumor-associated amyloidosis in dogs has been reported in several studies, but its incidence and predisposing conditions have not been investigated. We examined 252 mammary masses from 183 dogs to determine the prevalence of amyloid deposition and elucidate etiologic factors. Histologically, amyloid deposition was observed in 88 of 252 (35%) mammary masses. Amyloid deposition patterns were categorized into 3 types: stromal deposition (ST), deposition in corpora amylacea (CA), and precipitation within luminal secretions (PR). Of the 88 amyloid deposition cases, 49 were intra-tumoral, especially for ST-type amyloid deposits, which were all intra-tumoral. Immunohistochemistry and mass spectrometry revealed that ST-type amyloid deposits were positive for either serum amyloid A or α-S1-casein, depending on the sample; all CA- and PR-type amyloid deposits were positive for α-S1-casein. Statistically, intra-tumoral ST-type amyloid deposition was significantly more frequent in histologic grade II (p = 0.009) or III (p < 0.001) mammary tumors. In contrast, amyloid deposition in extra-tumoral tissues is significantly associated with aging (p = 0.002). Our findings suggest that the disruption of proteostasis in the canine mammary gland associated with tumorigenesis and aging can readily induce amyloid formation.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"574-583"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11966624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143763646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cytology and histology of a high-grade oligodendroglioma with embryonal features in a dog. 犬胚胎特征的高级别少突胶质细胞瘤的细胞学和组织学研究。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-04-16 DOI: 10.1177/10406387251331553

Flavio H Alonso, Aria Phan, Kelly O'Connell, Demitria M Vasilatis

引用次数: 0

Comparison of the determination of cortisol in canine saliva by chemiluminescence immunoassay and liquid chromatography-tandem mass spectrometry. 化学发光免疫分析法与液相色谱-串联质谱法测定犬唾液中皮质醇的比较。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-07-01 Epub Date: 2025-05-14 DOI: 10.1177/10406387251327413

Maike Schroers, Yuri Zablotski, Andrea Meyer-Lindenberg

{"title":"Comparison of the determination of cortisol in canine saliva by chemiluminescence immunoassay and liquid chromatography-tandem mass spectrometry.","authors":"Maike Schroers, Yuri Zablotski, Andrea Meyer-Lindenberg","doi":"10.1177/10406387251327413","DOIUrl":"10.1177/10406387251327413","url":null,"abstract":"We determined saliva cortisol concentrations (SCCs) in samples from 36 dogs using a chemiluminescence immunoassay (CLIA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) before and after a 10-min veterinary visit. The median cortisol concentration of the 77 samples analyzed with CLIA was 1.34 nmol/L, and the concentration of samples analyzed with LC-MS/MS was 1.30 nmol/L. With an intercept (a) of -0.06 (95% CI: -0.16 to 0.02) and a slope (b) of 0.97 (95% CI: 0.90-0.97) the Passing-Bablok regression revealed that there were no significant systematic and proportional differences between the 2 methods. In the Bland-Altman analysis, the bias was 0.08 nmol/L (95% CI: -0.38 to 0.54 nmol/L) between the 2 methods. Comparing the hormone levels in the saliva of 36 dogs before and after a veterinary visit, the CLIA method had a mean difference of 0.03 nmol/L (95% CI: -1.72 to 4.75 nmol/L; p = 0.35) and the LC-MS/MS method a mean difference of 0.12 nmol/L (95% CI 95%: -2.43 to 4.40 nmol/L; p = 0.62). Comparison of cortisol changes before (p = 0.86) and after (p = 0.79) the veterinary visit revealed no differences in using either the CLIA or LC-MS/MS methods. There was also no difference in the assessment of hormone increase or decrease between CLIA (p = 0.72) and LC-MS/MS (p = 0.17). Overall, the measurement of SCC in dogs by either CLIA or LC-MS/MS provided comparable results.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"539-544"},"PeriodicalIF":1.2,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081392/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of sampling and laboratory parameters contributing to successful isolation of senecavirus A1 from pigs. 对成功分离猪塞尼卡病毒A1的采样和实验室参数进行评估。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-06-23 DOI: 10.1177/10406387251327604

BoMyoung Kim, Munashe Chigerwe, Beate M Crossley

{"title":"Evaluation of sampling and laboratory parameters contributing to successful isolation of senecavirus A1 from pigs.","authors":"BoMyoung Kim, Munashe Chigerwe, Beate M Crossley","doi":"10.1177/10406387251327604","DOIUrl":"10.1177/10406387251327604","url":null,"abstract":"Senecavirus A1 (SVA; Picornaviridae, Senecavirus valles) infection causes an emerging infectious disease of swine that often leads to cutaneous vesicles on the snout and coronary bands and in the oral cavity of affected pigs, and causes epidemic transient neonatal loss in piglets. Virus isolation (VI) of SVA is required for viability testing, reference stock production, and monitoring virus evolution. We evaluated sampling and testing parameters leading to the successful propagation of SVA with a cross-sectional design based on 1,102 samples submitted to a reference laboratory. Logistic regression was used to evaluate the association between 16 variables and the outcome. When April was considered the reference month, the odds of SVA isolation failure were 17 and 100 times more likely during July and August, respectively. When foot samples were considered the referent, the odds of VI were 1.7 times more likely in unspecified samples. Compared to pooled samples, the odds of successful SVA isolation in individual samples were 8 and 45 times more likely in July and August, respectively. Laboratory parameters were not significantly associated with SVA isolation. The logistic model was moderately accurate (AUC = 0.68; 95% CI: 0.65-0.72%), suggesting that the model should be used cautiously for practical purposes. High environmental temperatures might negatively affect virus recovery. Using individual samples might improve SVA recovery when high ambient temperatures are recorded, as in the months of July and August.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251327604"},"PeriodicalIF":1.2,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12187694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient blocking ELISA for bovine alphaherpesvirus 1 using a gB epitope-specific monoclonal antibody. 使用gB表位特异性单克隆抗体高效阻断牛α疱疹病毒1的ELISA试剂盒。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-06-18 DOI: 10.1177/10406387251346909

Shuang-Yan Xiao, Feng-Dong Zhu, Dan-Jing Wang, Yan-Long Hu, Hui-Qin Jia, Huan-Chun Chen, Zheng-Fei Liu

{"title":"Efficient blocking ELISA for bovine alphaherpesvirus 1 using a gB epitope-specific monoclonal antibody.","authors":"Shuang-Yan Xiao, Feng-Dong Zhu, Dan-Jing Wang, Yan-Long Hu, Hui-Qin Jia, Huan-Chun Chen, Zheng-Fei Liu","doi":"10.1177/10406387251346909","DOIUrl":"10.1177/10406387251346909","url":null,"abstract":"Infectious bovine rhinotracheitis (IBR) is an infectious respiratory disease in cattle that is caused by bovine alphaherpesvirus 1 (BoAHV1). We immunized BALB/c mice with inactivated and purified BoAHV1 to prepare hybridoma cells. After the successful establishment of a positive hybridoma cell line, co-immunoprecipitation coupled with mass spectrometry unveiled the predominant targeting of glycoprotein B (gB) by the hybridoma cells. Through bioinformatics analysis and Western blot techniques, we identified the epitope of the monoclonal antibody (mAb) against gB to amino acids 1-170. Subsequently, the 1H3 mAb was leveraged for the development of a gB blocking ELISA (gB-bELISA), utilizing inactivated BoAHV1 virions as the coating antigen. The optimized protocol involved diluting samples 2-fold with 1% fish gelatin, followed by incubation periods of 120 min for samples, 30 min for HRP-conjugated 1H3 mAb, and 15 min for the TMB substrate. We validated our assay using 268 bovine serum samples with clear backgrounds and established the cutoff value of 43.8% through ROC analysis. Additionally, we tested 256 clinical bovine serum samples using both our gB-bELISA and a virus neutralization test, achieving a concordance rate of 95.3%. Based on testing 495 randomly selected sera from 18 counties for BoAHV1 antibodies with our gB-bELISA, the seroprevalence of IBR in the Central China region was 22.0% (95% CI: 18.4, 25.7). Our gB-bELISA could be a valuable tool for the clinical detection of IBR, supporting disease control and eradication efforts.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251346909"},"PeriodicalIF":1.2,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A retrospective study of benign epithelial salivary gland neoplasia in 11 dogs and 5 cats. 11只狗和5只猫涎腺良性上皮瘤的回顾性研究。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-06-12 DOI: 10.1177/10406387251329526

Daniel Felipe Barrantes Murillo, Emily J Brinker, Daniel R Rissi, Brian F Porter, Tatiane Terumi Negrão Watanabe

{"title":"A retrospective study of benign epithelial salivary gland neoplasia in 11 dogs and 5 cats.","authors":"Daniel Felipe Barrantes Murillo, Emily J Brinker, Daniel R Rissi, Brian F Porter, Tatiane Terumi Negrão Watanabe","doi":"10.1177/10406387251329526","DOIUrl":"10.1177/10406387251329526","url":null,"abstract":"Benign salivary gland neoplasms are infrequent in dogs and cats. The available literature is scarce, relying on scattered case series and case reports. The histologic subclassification of these neoplasms in humans is correlated with tumor outcome and is used as a template for the veterinary species. To better describe the clinical and histologic features of these benign epithelial tumors in dogs and cats, we performed a retrospective multi-institutional study and retrieved 16 benign epithelial salivary neoplasms from 11 dogs and 5 cats. In dogs, the sex distribution was 7 castrated males, 3 spayed females, and 1 intact female. The average age at diagnosis was 10.4 y (range: 4-15 y; ±3.0 SD). The diagnoses were canalicular adenoma (5 cases), pleomorphic adenoma (3 cases), cystadenoma (2 cases), and basal cell adenoma (1 case). In cats, the sex distribution was 4 spayed females and 1 castrated male. The average age at diagnosis was 11 y (range: 7-18 y; ±4.6 SD). Diagnoses were canalicular adenoma (4 cases) and cystadenoma (1 case). The diagnostic pathologist should be familiar with the current veterinary and human nomenclature of these neoplasms to make an accurate diagnosis.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251329526"},"PeriodicalIF":1.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing detection of porcine reproductive and respiratory syndrome virus RNA in processing fluids stored at room temperature using PrimeStore molecular transport medium. 利用PrimeStore分子运输介质提高对猪繁殖与呼吸综合征病毒RNA的检测。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-06-12 DOI: 10.1177/10406387251348464

Onyekachukwu Osemeke, Jerry Torrison, Christopher Helm, Michael Zeller, Rabsa Naseer, Claire Le Fevre, Grant Allison, James Mark Hammer, Giovani Trevisan, Phillip Gauger, Daniel Linhares

{"title":"Enhancing detection of porcine reproductive and respiratory syndrome virus RNA in processing fluids stored at room temperature using PrimeStore molecular transport medium.","authors":"Onyekachukwu Osemeke, Jerry Torrison, Christopher Helm, Michael Zeller, Rabsa Naseer, Claire Le Fevre, Grant Allison, James Mark Hammer, Giovani Trevisan, Phillip Gauger, Daniel Linhares","doi":"10.1177/10406387251348464","DOIUrl":"10.1177/10406387251348464","url":null,"abstract":"Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen affecting the U.S. swine industry, resulting in substantial costs associated with disease prevention, elimination, and control. Processing fluids (PFs) are an increasingly popular sample type for PRRSV surveillance due to their cost-effectiveness and high herd-level sensitivity. However, like other aggregate sample types, PF samples are non-pristine, and the RNA within them is susceptible to degradation, increasing the risk of false-negative and/or inconsistent results in reverse-transcription real-time PCR (RT-rtPCR) testing. We evaluated the efficacy of the nucleic acid-preserving reagent PrimeStore molecular transport medium (PS-MTM) in improving PRRSV RT-rtPCR detection rates, Ct values, and whole-genome sequencing (WGS) outcomes in PF samples. We collected PF samples from 2 PRRSV-positive sow herds and divided them into untreated controls and 2 PS-MTM-treated groups. PS-MTM-treated PFs had significantly lower Ct values compared to untreated samples, indicating improved RNA preservation and detectability. Although sequencing outcomes varied between study groups, PS-MTM-treated samples had statistically higher unique read counts. However, differences in other measured sequencing outcomes between the study groups were not statistically significant. PS-MTM has potential in PF sampling protocols to enhance PRRSV surveillance accuracy by reducing false-negative RT-rtPCR results.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251348464"},"PeriodicalIF":1.2,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12162531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization and validation of ELISAs for interferon-gamma determination in bison. 野牛血清干扰素测定elisa的优化与验证。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-06-03 DOI: 10.1177/10406387251344567

Josephine Chileshe, Todd Shury, Jeffrey M Chen

{"title":"Optimization and validation of ELISAs for interferon-gamma determination in bison.","authors":"Josephine Chileshe, Todd Shury, Jeffrey M Chen","doi":"10.1177/10406387251344567","DOIUrl":"10.1177/10406387251344567","url":null,"abstract":"Bovine tuberculosis, caused by Mycobacterium bovis, is endemic in the Wood Buffalo National Park, Canada, home to free-ranging and threatened wood bison. This disease poses a threat to the conservation of this culturally and ecologically important animal species, as well as potentially impacting the health of humans and other animal species via zoonosis and spillover, respectively. The ability to detect infection early will minimize and prevent the potential risk of M. bovis transmission. Interferon-gamma (IFNγ) assays are a reliable detection method for M. bovis in cattle and other wildlife species and may have diagnostic value in bison as well. We aimed to optimize and partially validate 2 commercial IFNγ ELISAs to detect endogenous bison IFNγ in mitogen-stimulated whole blood. Parameters evaluated included antibody identification, sample matrix effect, dilution linearity, assay reproducibility, and limit of quantification. The optimized assays demonstrated linear responses to recombinant bovine and endogenous bison IFNγ (range: 1-125 pg/mL; R2 = 0.99), with good recovery and fair reproducibility, and a low limit of quantification of 1 pg/mL. Mabtech bovine Flex and Pro kits have the same antibodies but in 2 different assay formats; an in-house assay platform (Flex kit) and precoated plates (Pro kit) are considered suitable for measuring bison IFNγ, offering flexibility depending on available resources.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251344567"},"PeriodicalIF":1.2,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12133778/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diseases of the nervous system of equids in Brazil: a review. 巴西马科动物神经系统疾病综述。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-05-30 DOI: 10.1177/10406387251325881

Kesia S Carvalho, Claudio S L de Barros, Fabio S Mendonça, Mizael Machado, Franklin Riet-Correa

{"title":"Diseases of the nervous system of equids in Brazil: a review.","authors":"Kesia S Carvalho, Claudio S L de Barros, Fabio S Mendonça, Mizael Machado, Franklin Riet-Correa","doi":"10.1177/10406387251325881","DOIUrl":"10.1177/10406387251325881","url":null,"abstract":"In Brazil, CNS diseases in equids were little known until the 1980s. Since then, several diagnostic laboratories have been operating in different universities, initially in the South and Southeast regions and, later, in the Central-West, Northeast and North regions. However, the knowledge accumulated from the diagnoses of nervous system diseases of equids made at these institutions over the years has not been reviewed comprehensively, and many papers are published in Portuguese. Here, we review 18 diseases that occur in the nervous system of equids in Brazil, including some critical infectious diseases (rabies, equine encephalomyelitides, equine herpesviral myeloencephalopathy, protozoan myeloencephalitis), and diseases caused by toxic plants and mycotoxins. Our review provides reference data to assist pathologists and clinicians in diagnosing neurologic diseases in equids. We also hope it will serve as a reference for professionals in this field abroad, allowing them to compare data in the epidemiology and pathology of the nervous system diseases of equids in different regions of the world.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251325881"},"PeriodicalIF":1.2,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12125018/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perirenal hemorrhage associated with feline infectious peritonitis: a novel presentation of a classic disease. 与猫感染性腹膜炎相关的肾周出血：一种经典疾病的新表现。

IF 1.2 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-05-29 DOI: 10.1177/10406387251341239

Madeleine I Gauthier, Carolyn Legge, Dayna A Goldsmith, Maria Bravo Araya, Jennifer L Davies

{"title":"Perirenal hemorrhage associated with feline infectious peritonitis: a novel presentation of a classic disease.","authors":"Madeleine I Gauthier, Carolyn Legge, Dayna A Goldsmith, Maria Bravo Araya, Jennifer L Davies","doi":"10.1177/10406387251341239","DOIUrl":"10.1177/10406387251341239","url":null,"abstract":"Feline infectious peritonitis (FIP), caused by a mutated biotype of feline coronavirus (FCoV; Coronaviridae, Alphacoronavirus), is a significant disease of felids. We investigated perirenal hemorrhage, an unreported lesion in FIP, through a retrospective analysis of 51 immunohistochemistry-confirmed FIP cases submitted to the Diagnostic Services Unit (DSU; University of Calgary, Calgary, Alberta, Canada) between 2010 June 30 and 2024 June 30. Five cats had perirenal hemorrhage in the right retroperitoneal space; 4 had concurrent subcapsular renal hemorrhage; and 1 had sublumbar muscle hemorrhage and hemoabdomen. One case had additional hemorrhages in the brain and cervical spinal cord. Concurrent gross lesions typical of FIP included pyogranulomatous inflammation in various organs and protein-rich cavitary effusions. Histologic lesions typical of FIP (vasculitis and pyogranulomatous inflammation) were present in the kidneys and retroperitoneal fat of 4 cases, and in 3 cases, FCoV antigen was demonstrated in the regions of hemorrhage. The exact mechanism of this hemorrhage is unknown, but we speculate that vasculitis caused by FIP is the cause. Despite the relatively low prevalence of perirenal hemorrhage in this cohort, this lesion represents a unique, previously unreported manifestation of FIP that clinicians and pathologists should be aware of and consider in the differential diagnosis for fluid accumulation or space-occupying lesions in the retroperitoneum of cats.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387251341239"},"PeriodicalIF":1.2,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0