Journal of Veterinary Diagnostic Investigation最新文献_第5页

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-11-01 Epub Date: 2026-07-11 DOI: 10.1177/10406387261455822

Yolanda Gómez Vargas, Jahir O Vargas Domínguez, Jorge G Noriega Márquez, Nathalia M Correa-Valencia, Diego Ortiz Ortega

{"title":"Age-related Mycobacterium avium subsp. paratuberculosis seroprevalence in Colombian cattle across 6 ecologic life zones.","authors":"Yolanda Gómez Vargas, Jahir O Vargas Domínguez, Jorge G Noriega Márquez, Nathalia M Correa-Valencia, Diego Ortiz Ortega","doi":"10.1177/10406387261455822","DOIUrl":"10.1177/10406387261455822","url":null,"abstract":"Mycobacterium avium subsp. paratuberculosis (MAP) prevalence estimates vary widely across Colombian regions, with no prior longitudinal assessment of Colombian dairy systems across ecologic life zones. We assessed the temporal dynamics of MAP infection in Colombian cattle across 6 distinct ecologic life zones and explored age-related differences in MAP detection using combined serologic and molecular approaches. We longitudinally sampled 129 cattle from 6 municipalities in distinct Holdridge life zones. The animals were stratified by age group and sampled 3 times at 2-mo intervals during 2023 (Aug-Dec). Serum and fecal samples were tested by indirect ELISA and IS900 real-time PCR (rtPCR), respectively. The apparent ELISA prevalence remained stable between 37.2% and 38% across sampling rounds (adjusted true prevalence = 29.9%). Seropositivity increased consistently with age, from 11.9% in animals <1-mo-old to 49% in animals ≥3-y-old. All of the fecal samples tested negative by rtPCR. No consistent seroprevalence clustering was observed across ecologic life zones on descriptive assessment, although management and host factors were not evaluated. We found longitudinal evidence of stable, age-related MAP seroprevalence across ecologic zones in Colombia, highlighting subclinical persistence and diagnostic limitations.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1345-1349"},"PeriodicalIF":1.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13356030/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148422139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Meningoencephalitis and systemic infection by Acanthamoeba sp. in cattle in Brazil. 巴西牛棘阿米巴属脑膜脑炎和全身感染。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-11-01 Epub Date: 2026-07-14 DOI: 10.1177/10406387261462864

Larissa L Souza, Bruno S Rezende, Rayane C Pupin, Herbert P K Cleveland, Carlos N Ramos, Ricardo A A Lemos, Francisco A Uzal, Danilo C Gomes

{"title":"Meningoencephalitis and systemic infection by Acanthamoeba sp. in cattle in Brazil.","authors":"Larissa L Souza, Bruno S Rezende, Rayane C Pupin, Herbert P K Cleveland, Carlos N Ramos, Ricardo A A Lemos, Francisco A Uzal, Danilo C Gomes","doi":"10.1177/10406387261462864","DOIUrl":"10.1177/10406387261462864","url":null,"abstract":"Free-living amebas such as Acanthamoeba spp., Balamuthia mandrillaris, and Naegleria fowleri can cause infections in humans and animals. Here, we describe 2 cases of meningoencephalitis and an outbreak of multisystemic disease caused by Acanthamoeba sp. in cattle from Central-Western Brazil. Within 3 d of onset, 2 adult cattle from 2 different herds developed acute neurologic signs, including incoordination progressing to flaccid paralysis, opisthotonos, recumbency, and spontaneous death (case 1), and blindness, behavioral alterations, and flaccid paralysis leading to euthanasia (case 2). In a separate herd, 2 of 11 calves died within 5 d of developing lethargy and incoordination and were autopsied (cases 3, 4). On gross examination, the brains of all 4 cases and the cervicothoracic spinal cord of case 3 had red-brown areas of malacia and cavitation. Case 3 also had myriad firm, white, spherical nodules in the kidneys; case 4 had numerous firm, white lung nodules. Histologically, all animals had multifocal necrosis with granulomatous inflammation and numerous amebic trophozoites and cysts in the CNS. Lesions also included pyogranulomatous pneumonia, nephritis, myocarditis, and splenitis. Immunohistochemistry and PCR, followed by sequencing, confirmed Acanthamoeba spp. infection in all 4 cases. To our knowledge, Acanthamoeba sp.-associated encephalitis and multisystemic disease have not been reported previously in cattle.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"1361-1365"},"PeriodicalIF":1.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13372793/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148447785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systemic fibrinogen Aα-chain amyloidosis in 3 Japanese raccoon dogs. 3只日本貉系统性纤维蛋白原a α链淀粉样变性的研究。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-10-27 DOI: 10.1177/10406387261493975

Momoko Yoshikura, Natsumi Kobayashi, Mitsuhiro Ikeda, Yoshiyuki Itoh, Daisuke Nakagawa, Hirotaka Kondo, Tomoaki Murakami

引用次数: 0

Catastrophic distal sesamoidean ligament rupture in 72 racehorses in California. 加州72匹赛马的远端海马韧带灾难性断裂。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-10-27 DOI: 10.1177/10406387261493577

Carlos O Schild, Hernando D Acevedo, Jeanette I Espil, Jeff A Blea, Javier Asin, Eileen E Henderson, Akinyi Nyaoke, Laura A Kennedy, Susan M Stover, Francisco A Uzal

{"title":"Catastrophic distal sesamoidean ligament rupture in 72 racehorses in California.","authors":"Carlos O Schild, Hernando D Acevedo, Jeanette I Espil, Jeff A Blea, Javier Asin, Eileen E Henderson, Akinyi Nyaoke, Laura A Kennedy, Susan M Stover, Francisco A Uzal","doi":"10.1177/10406387261493577","DOIUrl":"https://doi.org/10.1177/10406387261493577","url":null,"abstract":"Rupture of major distal sesamoidean ligaments (DSLs) in racehorses results in suspensory apparatus failure. Our objective was to describe postmortem findings and exercise histories for Thoroughbred (TB) and Quarter Horse racehorses that had DSL rupture as the sole cause of catastrophic musculoskeletal and suspensory apparatus failure. We described horse signalment, activity at the time of injury, and postmortem findings and compared the exercise histories with those of event-matched control horses. In 72 racehorses, predominantly ≥4-y-old, catastrophic rupture of forelimb DSLs (41 left, 29 right, 2 bilateral) occurred while racing (61), training (9), or during non-exercise activity (2). DSL rupture occurred in isolation in 27 horses; the remaining 45 horses also had disruption of other ligaments and tendons secondary to associated fetlock or pastern disarticulation. Rupture of the DSLs involved the straight (69), oblique (68 medial, 69 lateral), cruciate (49), and short (49) DSLs. Complete rupture of the straight, oblique, cruciate, and short DSLs occurred more frequently (p < 0.05) in the proximal third of the pastern region (49 horses) than complete rupture of the straight and oblique DSLs in the distal third of the pastern region (23 horses). Two horses had chronic desmopathy in the proximal third of the oblique DSL. Proximal ruptures occurred more frequently in the left forelimb (p = 0.005). TB horses with DSL rupture had longer careers, more time in layup, and less high-speed activity in the year before injury than unaffected control horses. Complete rupture of the DSLs had a very low prevalence (1.4%) among catastrophic musculoskeletal injuries.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261493577"},"PeriodicalIF":1.4,"publicationDate":"2026-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149269408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Presumed myofibroblastic hepatic sarcoma associated with a retained surgical swab in a 3.5-year-old dog. 在一只3.5岁的狗身上发现残留手术拭子，推测为肝肌纤维母细胞肉瘤。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-10-26 DOI: 10.1177/10406387261493977

Dávid G Horváth, Patrik Tráj, Zsolt Virág, Márton Márialigeti, Fanni Vrabély, Csaba Jakab, Gyula Balka

{"title":"Presumed myofibroblastic hepatic sarcoma associated with a retained surgical swab in a 3.5-year-old dog.","authors":"Dávid G Horváth, Patrik Tráj, Zsolt Virág, Márton Márialigeti, Fanni Vrabély, Csaba Jakab, Gyula Balka","doi":"10.1177/10406387261493977","DOIUrl":"https://doi.org/10.1177/10406387261493977","url":null,"abstract":"A 3.5-y-old spayed female Labrador Retriever was presented with inappetence, weakness, vomiting, and a tense abdomen. Via abdominal ultrasonography, radiography, and diagnostic laparotomy, we identified a hepatic mass. Thereafter, we performed a lobectomy and sent the resected specimen for histologic examination. The dog was euthanized given the lack of clinical improvement and the formation of an intrathoracic soft-tissue mass identified with radiography. Histologically, the thoracic mass was an infiltrative, highly cellular mesenchymal neoplasm. Its spindle cells were arranged in interwoven fascicles and bundles with marked anisocytosis, anisokaryosis, and nuclear atypia, and a high mitotic count. The tumor tissue surrounded large necrotic areas with numerous cotton fibers originating from a retained surgical swab, which had elicited a marked granulomatous inflammatory response. The neoplastic cells uniformly expressed strong cytoplasmic vimentin immunolabeling. Approximately 50% of the neoplastic cells had strong cytoplasmic \"tram-track\" α-SMA and moderate cytoplasmic calponin immunolabeling. Tumor cells did not have desmin, S100, CD31, factor VIII-related antigen (von Willebrand factor), HepPar1, or IBA1 immunolabeling. The microscopic and immunohistochemical features were consistent with a soft-tissue sarcoma, most likely a myofibroblastic sarcoma. Hepatic myofibroblastic sarcoma related to retained surgical swab has not been reported previously in dogs, to our knowledge, nor has the use of HepPar1 immunohistochemistry to investigate the possible hepatocellular origin of the tumor.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261493977"},"PeriodicalIF":1.4,"publicationDate":"2026-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149269517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypervirulent Listeria isolated from a wild rabbit in Canada. 从加拿大一只野兔中分离出高毒力李斯特菌。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-10-24 DOI: 10.1177/10406387261493575

Audrey F Widjaja, Claire M Jardine, Brian Stevens, Chantal Theijn, Durda Slavic, Veronica Guariglia-Oropeza

{"title":"Hypervirulent Listeria isolated from a wild rabbit in Canada.","authors":"Audrey F Widjaja, Claire M Jardine, Brian Stevens, Chantal Theijn, Durda Slavic, Veronica Guariglia-Oropeza","doi":"10.1177/10406387261493575","DOIUrl":"https://doi.org/10.1177/10406387261493575","url":null,"abstract":"Listeria monocytogenes is the causative agent of listeriosis, a major foodborne disease with a 30% mortality rate in humans. Although L. monocytogenes can infect animals, whether wildlife can serve as a reservoir remains understudied, especially in Canada. Here, we characterized an L. monocytogenes strain (W0463-24) extracted from a wild cottontail rabbit (Sylvilagus floridanus) submitted to the Canadian Wildlife Health Cooperative at the University of Guelph. The rabbit had severe swelling of the head, multiple scabs around the muzzle and eyes, and was diagnosed with disseminated lymphoma, multifocal necrotizing hepatitis with gram-positive bacteria, and nematodosis. Liver and spleen were PCR-negative for Francisella tularensis, and liver was 4+ for L. monocytogenes on bacterial culture. Whole-genome analysis characterized isolate W0463-24 as sequence type 1 (ST1), clonal complex 1 (CC1), and lineage I. Multiple virulence and stress response genes were identified in silico, including the Listeria pathogenicity island 3 (LIPI-3). Invasion of human colon adenocarcinoma (Caco-2) cells in vitro was significantly greater with W0463-24 compared with wild-type strain 10403S. We found that this ST1, CC1, lineage I wildlife isolate has high pathogenic potential, emphasizing wildlife both as potential reservoirs and as populations vulnerable to L. monocytogenes infection.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261493575"},"PeriodicalIF":1.4,"publicationDate":"2026-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13695606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149269381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Postmortem findings in racehorses from the U.S. Mid-Atlantic region submitted to a Pennsylvania veterinary diagnostic laboratory. 美国中大西洋地区赛马的尸检结果提交给宾夕法尼亚州兽医诊断实验室。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-10-23 DOI: 10.1177/10406387261485572

Joanne E Haughan, Mary Kate Grubbs, Cadence Kelly, Lauren Pittman, Darko Stefanovski, Mary A Robinson, Julie B Engiles

{"title":"Postmortem findings in racehorses from the U.S. Mid-Atlantic region submitted to a Pennsylvania veterinary diagnostic laboratory.","authors":"Joanne E Haughan, Mary Kate Grubbs, Cadence Kelly, Lauren Pittman, Darko Stefanovski, Mary A Robinson, Julie B Engiles","doi":"10.1177/10406387261485572","DOIUrl":"https://doi.org/10.1177/10406387261485572","url":null,"abstract":"We performed a retrospective analysis of postmortem reports for racehorses submitted to the Pennsylvania Animal Diagnostic Laboratory System between July 2017 and July 2025. We included 394 (366 Thoroughbred [TB], 28 Standardbred [SB]) racehorses, 2-11-y-old (median = 4-y-old), from the Mid-Atlantic region. Annually, 34-58 TBs and 1-9 SBs were submitted. TB racehorse submissions were highest in July and October; SB racehorse submissions were highest in June and July. Approximately two-thirds of submitted racehorses died or were euthanized within 1 h of racing or training. Catastrophic musculoskeletal (MSK) injury was the highest reported cause of fatality for both TB (272 of 366; 74%) and SB (15 of 28; 54%) cohorts. In TBs, 245 of 272 (90%) of catastrophic MSK injuries were appendicular skeletal fractures, most involving the forelimb fetlock joints. Fractures of the proximal sesamoid bones predominated, occurring alone or as components of polyostotic fetlock fractures. In SBs, 15 of 15 (100%) catastrophic MSK injuries were appendicular skeletal fractures, most commonly involving the proximal phalanx of hindlimb fetlock joints. Undetermined cause of death was the second highest reported cause of fatalities in both TBs (31 of 366; 8%) and SBs (5 of 28; 18%). Fatalities were rarely the result of non-catastrophic MSK disease or diseases involving other organ systems. Our results are similar to those of published reports from other regions and may help inform research initiatives focused on early disease detection or prevention strategies to reduce the risk of racehorse fatalities and improve equine welfare.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261485572"},"PeriodicalIF":1.4,"publicationDate":"2026-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13694455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149269458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infectious necrotic hepatitis caused by Clostridium novyi type B in a goat with listeriosis. 李斯特菌病山羊新梭菌B型引起的感染性坏死性肝炎。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-10-14 DOI: 10.1177/10406387261493971

Claudia A Márquez-Flández, Javiera B Becker-Cárdenas, Eliser A Mansilla, Francisco A Uzal, Mauricio A Navarro

{"title":"Infectious necrotic hepatitis caused by Clostridium novyi type B in a goat with listeriosis.","authors":"Claudia A Márquez-Flández, Javiera B Becker-Cárdenas, Eliser A Mansilla, Francisco A Uzal, Mauricio A Navarro","doi":"10.1177/10406387261493971","DOIUrl":"10.1177/10406387261493971","url":null,"abstract":"A 6-y-old postpartum Nubian crossbreed goat was submitted for postmortem examination following a clinical course of ataxia, lateral head tilt, and recumbency. The liver had a poorly defined, pale-yellow focally extensive lesion and focal thickening of bile ducts. Histologically, an area of extensive coagulative necrosis was surrounded by many viable and degranulated neutrophils and gram-positive bacilli, which were identified as Clostridium novyi by immunohistochemistry. Numerous neutrophilic infiltrates were observed in the brainstem. PCR on DNA extracted from hepatic tissue was positive for the fliC, tcna, and tcnb genes of C. novyi type B. Additionally, PCR testing of brainstem tissue was positive for the hlyA gene of Listeria monocytogenes. To our knowledge, infectious necrotic hepatitis (INH), confirmed by molecular testing and with concurrent listeriosis, has not been reported previously in a goat. Clostridial hepatitis in animals is typically triggered by anaerobic conditions within the liver parenchyma, which facilitate the germination of dormant spores and the subsequent release of potent toxins. Although flukes were not observed, the presumptive inciting hypoxic event leading to parenchymal necrosis in this case was Fasciola hepatica larval migration. Because the clinical and pathology features of INH caused by C. novyi type B and bacillary hemoglobinuria caused by C. haemolyticum are nearly identical, molecular differentiation is essential to establish an accurate etiologic diagnosis.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261493971"},"PeriodicalIF":1.4,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13669245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caprine astrovirus detected in conjunction with necrotizing enterocolitis in goat kids in Indiana. 在印第安纳州山羊儿童中检测到与坏死性小肠结肠炎相关的山羊星状病毒。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-10-05 DOI: 10.1177/10406387261488858

Grant N Burcham, Torsten Seuberlich, Ganwu Li, Angela S Chan, Kara L D Dawson

{"title":"Caprine astrovirus detected in conjunction with necrotizing enterocolitis in goat kids in Indiana.","authors":"Grant N Burcham, Torsten Seuberlich, Ganwu Li, Angela S Chan, Kara L D Dawson","doi":"10.1177/10406387261488858","DOIUrl":"10.1177/10406387261488858","url":null,"abstract":"An outbreak of enteric disease occurred in a herd of Nigerian Dwarf goats in Indiana, USA, in March 2024. Kids 2-5-wk-old had severe diarrhea that did not respond to treatment. Two kids that underwent postmortem examination had gross and histologic evidence of fibrinonecrotizing typhlocolitis. Ancillary testing for known causes of enterocolitis in goats did not yield any potential causes. High-throughput sequencing (HTS) was performed to identify possible novel viral pathogens. Total nucleic acids were extracted from fresh intestine for HTS analysis, and a subset of astrovirus reads was obtained and assembled. This result was confirmed by HTS of total RNA extracted from formalin-fixed paraffin-embedded material in a separate laboratory, enabling the assembly of a full-genome astrovirus sequence of 6,285 nucleotides. This novel astrovirus strain was designated caprine astrovirus (CapAstV; family Astroviridae, genus Mamastrovirus) Indiana/2024. No other viruses were identified via either method. In situ hybridization for CapAstV Indiana/2024 RNA was performed to link the pathogen and lesion. Positive signal was detected within the enterocyte cytoplasm in intestinal sections, as well as presumed macrophages within the lamina propria. We confirmed that CapAstV Indiana/2024 infected goat enterocytes and occurred in conjunction with severe necrotizing enteric disease.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261488858"},"PeriodicalIF":1.4,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13646206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149110957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of highly pathogenic avian influenza A(H5N1) virus, clade 2.3.4.4b, genotype B3.13 in placentas from aborted bovine fetuses. 牛流产胎胎盘中检测高致病性禽流感A（H5N1）病毒，进化枝2.3.4.4b，基因型B3.13。

IF 1.4 3区农林科学

Journal of Veterinary Diagnostic Investigation Pub Date : 2026-09-30 DOI: 10.1177/10406387261483207

Melissa Macías-Rioseco, Sharif S Aly, Javier Asin, Todd Cornish, Jennine Ochoa, Deniece R Williams, Raúl A Resendíz-Pozos, Robert B Moeller, Wendi Jackson, Wagdy R ElAshmawy, Beate Crossley, Francisco A Uzal

{"title":"Detection of highly pathogenic avian influenza A(H5N1) virus, clade 2.3.4.4b, genotype B3.13 in placentas from aborted bovine fetuses.","authors":"Melissa Macías-Rioseco, Sharif S Aly, Javier Asin, Todd Cornish, Jennine Ochoa, Deniece R Williams, Raúl A Resendíz-Pozos, Robert B Moeller, Wendi Jackson, Wagdy R ElAshmawy, Beate Crossley, Francisco A Uzal","doi":"10.1177/10406387261483207","DOIUrl":"https://doi.org/10.1177/10406387261483207","url":null,"abstract":"Infection by highly pathogenic avian influenza (HPAI) A(H5N1) virus (family Orthomyxoviridae, taxon species Alphainfluenzavirus influenzae), clade 2.3.4.4b, genotype B3.13, was first diagnosed in dairy cattle in the United States in March 2024. Here, we present a cluster of bovine abortions in whose placentas HPAI A(H5N1) was detected. Only placentas were submitted in all 5 cases received. Grossly, 3 placentas had multifocal edema; no significant gross abnormalities were observed in the other 2 placentas. Microscopically, one placenta had random areas of mineralization and another placenta had mild neutrophilic placentitis. Reverse-transcription quantitative real-time PCR for influenza A virus and avian influenza A virus subtype H5N1 were positive in all 5 placentas. RNAscope in-situ hybridization for matrix protein gene was positive in one placenta. Routine ancillary tests for bovine abortion diagnostic work-up did not detect any other abortifacient pathogens. We speculate that abortion was caused by the HPAI A(H5N1) virus.","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":" ","pages":"10406387261483207"},"PeriodicalIF":1.4,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13631111/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149111307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0